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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 1,009 records · Page 56
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration

Efficient genome engineering is critical to understand and use microbial functions. Despite recent development of tools such as CRISPR-Cas gene editing, efficient integration of exogenous DNA with well-characterized functions remains limited to model bacteria. Here, we describe serine recombinase–assisted genome engineering, or SAGE, an easy-to-use, highly efficient, and extensible technology that enables selection marker–free, site-specific genome integration of up to 10 DNA constructs, often with efficiency on par with or superior to replicating plasmids. SAGE uses no replicating plasmids and thus lacks the host range limitations of other genome engineering technologies. We demonstrate the value of SAGE by characterizing genome integration efficiency in five bacteria that span multiple taxonomy groups and biotechnology applications and by identifying more than 95 heterologous promoters in each host with consistent transcription across environmental and genetic contexts. We anticipate that SAGE will rapidly expand the number of industrial and environmental bacteria compatible with high-throughput genetics and synthetic biology.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Joint iterative reconstruction and 3D rigid alignment for X-ray tomography

X-ray tomography is widely used for three-dimensional structure determination in many areas of science, from the millimeter to the nanometer scale. The resolution and quality of the 3D reconstruction is limited by the availability of alignment parameters that correct for the mechanical shifts of the sample or sample stage for the images that constitute a scan. In this paper we describe an algorithm for marker-free, fully automated and accurately aligned and reconstructed X-ray tomography data. Our approach solves the tomographic reconstruction jointly with projection data alignment based on a rigid-body deformation model. We demonstrate the robustness of our method on both synthetic phantom and experimental data and show that our method is highly efficient in recovering relatively large alignment errors without prior knowledge of a low resolution approximation of the 3D structure or a reasonable estimate of alignment parameters.

36 MATERIALS SCIENCE↗
DOE OSTI2022

Iterative wavefront optimization of ultrafast laser beams carrying orbital angular momentum

Structured intense laser beams offer degrees of freedom that are highly attractive for high-field science applications. However, the performance of high-power laser beams in these applications is often hindered by deviations from the desired spatiotemporal profile. This study reports the wavefront optimization of ultrafast Laguerre-Gaussian beams through the synergy of adaptive optics and genetic algorithm-guided feedback. The results indicate that the intensity fluctuations along the perimeter of the target ring-shaped profile can be reduced up to ~15%. Furthermore, the radius of the ring beam profile can be tailored to a certain extent by establishing threshold fitting criteria. The versatility of this approach is experimentally demonstrated in conjunction with different focusing geometries.

47 OTHER INSTRUMENTATION↗
DOE OSTI2015

A model of lipid-free Apolipoprotein A-I revealed by iterative molecular dynamics simulation

Apolipoprotein A-I (apo A-I), the major protein component of high-density lipoprotein, has been proven inversely correlated to cardiovascular risk in past decades. The lipid-free state of apo A-I is the initial stage which binds to lipids forming high-density lipoprotein. Molecular models of lipid-free apo A-I have been reported by methods like X-ray crystallography and chemical cross-linking/mass spectrometry (CCL/MS). Through structural analysis we found that those current models had limited consistency with other experimental results, such as those from hydrogen exchange with mass spectrometry. Through molecular dynamics simulations, we also found those models could not reach a stable equilibrium state. Therefore, by integrating various experimental results, we proposed a new structural model for lipidfree apo A-I, which contains a bundled four-helix N-terminal domain (1–192) that forms a variable hydrophobic groove and a mobile short hairpin C-terminal domain (193–243). This model exhibits an equilibrium state through molecular dynamics simulation and is consistent with most of the experimental results known from CCL/MS on lysine pairs, fluorescence resonance energy transfer and hydrogen exchange. This solution-state lipid-free apo A-I model may elucidate the possible conformational transitions of apo A-I binding with lipids in high-density lipoprotein formation.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2014

High Flux FRC Facility for the Stability, Confinement and ITER Divertor Studies

The TCS (Translation, Confinement, & Sustainment) program was begun on 7 August, 1996 to renew basic studies of the Field Reversed Configuration (FRC). The program made use of the old LSX (Large s Experiment) device, which was constructed at STI during the period from 1986 to 1990, but only operated for one year due to a DOE decision at the time to focus exclusively on the tokamak configuration. LSX was transferred to the University of Washington in 1992 and modified (LSX/mod) to perform Tokamak Refueling by Accelerated Plasmoids (TRAP) experiments. The TRAP program was funded from 7 August, 1992 until 6 August, 1996, but was utilized for an additional year while TCS was being constructed. During the first TCS funding period TCS was completed and initial experiments were begun. A large multi-megawatt RF power supply was built by Los Alamos National Laboratory (LANL) for use with a Rotating Magnetic Field (RMF) system, and LANL has been a continuing participant in our experimental program. A smaller prototype facility, called the Star Thrust Experiment (STX) was also built and operated in this period, partly with NASA funding, before TCS came on-line. A final report for this construction period was submitted in September 2000. A first renewal period (2.5 years) provided operating funds for the period between July 7, 2000 and January 6, 2003. A great deal of progress was made in understanding the use of RMF to both form and sustain FRCs during this period. The principal result of the experimental program was the formation of quasi steady-state (as long as RMF power was available) FRCs with densities in the 1-3x10 19 m -3 range. However, the plasma temperature (T e or T i ) was limited to sub-25 eV, except transiently during start-up, by the rapid accumulation of impurities. This is not surprising since TCS was only designed to demonstrate RMF flux build-up and was not provided with either fueling capabilities or modern vacuum conditioning technology. (The unplanned for long time steady-state operation was due entirely to recycling.) TCS employed a multi-section quartz vacuum vessel with greased “O”-ring seals. A final report for this second funding period was submitted in May of 2003.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2014

IDC Reengineering Iteration I2 Architectural Prototype Reports

To fulfill the inception phase deliverable “Demonstration of architectural prototype“ the SNL IDC Reengineering project team is providing seven reports describing system prototyping work completed between October 2012 and October 2014as part of the SNL US NDC Modernization project.

96 KNOWLEDGE MANAGEMENT AND PRESERVATION↗
DOE OSTI2014

Non-iterative Voltage Stability

This report demonstrates promising capabilities and performance characteristics of the proposed method using several power systems models. The new method will help to develop a new generation of highly efficient tools suitable for real-time parallel implementation. The ultimate benefit obtained will be early detection of system instability and prevention of system blackouts in real time.

24 POWER TRANSMISSION AND DISTRIBUTION↗