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At least 991 records · Page 55

The effect of substrate temperature on the critical velocity in microparticle impact bonding

A number of nascent coating and micromanufacturing processes involve particles sprayed at a high velocity, which impact a substrate, deform, and adhere. Successful bonding between the particles and the substrate requires impact velocities higher than the so-called critical adhesion velocity. This critical velocity is influenced by the temperature of the substrate, a variable that we isolate in this work by conducting single-particle impacts on a variable-temperature substrate for three systems (Al–Al, Sn–Sn, and Ti–Ti) with particles individually selected within a narrow size distribution. Our results quantitatively connect the increase in substrate temperature to a significant lowering of the critical velocity, which we attribute to the lower dynamic strength of the thermally softened substrate. The data are generally consistent with expectations for bonding being controlled by a hydrodynamic process of jetting upon impact.

36 MATERIALS SCIENCE↗

Structural basis for divergent and convergent evolution of catalytic machineries in plant aromatic amino acid decarboxylase proteins

Radiation of the plant pyridoxal 5'-phosphate (PLP)-dependent aromatic L -amino acid decarboxylase (AAAD) family has yielded an array of paralogous enzymes exhibiting divergent substrate preferences and catalytic mechanisms. Plant AAADs catalyze either the decarboxylation or decarboxylation-dependent oxidative deamination of aromatic L -amino acids to produce aromatic monoamines or aromatic acetaldehydes, respectively. These compounds serve as key precursors for the biosynthesis of several important classes of plant natural products, including indole alkaloids, benzylisoquinoline alkaloids, hydroxycinnamic acid amides, phenylacetaldehyde-derived floral volatiles, and tyrosol derivatives. In this paper, we present the crystal structures of four functionally distinct plant AAAD paralogs. Through structural and functional analyses, we identify variable structural features of the substrate-binding pocket that underlie the divergent evolution of substrate selectivity toward indole, phenyl, or hydroxyphenyl amino acids in plant AAADs. Moreover, we describe two mechanistic classes of independently arising mutations in AAAD paralogs leading to the convergent evolution of the derived aldehyde synthase activity. Applying knowledge learned from this study, we successfully engineered a shortened benzylisoquinoline alkaloid pathway to produce (S)-norcoclaurine in yeast. This work highlights the pliability of the AAAD fold that allows change of substrate selectivity and access to alternative catalytic mechanisms with only a few mutations.

59 BASIC BIOLOGICAL SCIENCES↗

Mixed Element Microparticle Characterization by Electron Probe Microanalysis

Abstract Microparticle compositional characterization for nuclear forensics has traditionally been achieved by “gold-standard” destructive analytical techniques such as large geometry-secondary ion mass spectrometry and fission track-thermal ionization mass spectrometry, but long processing times and total sample consumption eliminate the option of subsequent analysis by other methods. Electron probe microanalysis has long been widely employed for rapid, nondestructive, micrometer-scale compositional measurements and imaging in fields such as material science and geology and may therefore need to be reevaluated as an alternative tool for microparticle analysis for the nuclear forensics community. This study presents the use of electron probe microanalysis for imaging and quantitative characterization of homogeneous microparticles utilizing a calibration curve based on high-precision quadrupole-inductively coupled plasma-mass spectrometry analyses. Samples of opportunity synthesized at Savannah River National Laboratory, nickel-doped cerium oxide microparticles, were selected as analogs for plutonium-doped uranium oxide microparticles. Positive detection and accurate quantification of variable amounts of nickel dopant down to trace levels (10s of parts per million) suggest applicability of this technique to other mixed element systems (e.g., actinides). Quantitative single-particle characterization via electron probe microanalyzer may thus provide a high fidelity, nondestructive complement to techniques currently in use.

Riche, Alexis T. (ORCID:0009000121978126)↗

An integrated integral projection model ( IPM 2 ) to disentangle size‐structured harvest and natural mortality

Abstract Body size is one of the most important traits governing individual‐level demographic rates and modulating population‐level processes. Multiple size‐dependent demographic rates can simultaneously change population structure, so distinguishing their individual contributions to overall population dynamics remains a challenge. Disentangling size‐dependent harvest rates from other demographic rates is critical for assessing the impact of removal on populations of invasive species. Inference about invasive populations can be difficult, however, as observations are often collected opportunistically as part of removal programs, rather than experimentally designed. Yet accurate inference is essential for understanding the feasibility of population suppression and optimising management decisions. We develop an integrated integral projection model (IPM 2 ) that leverages the strengths of the integrated population model and integral projection model to enable inference about complex, size‐structured demographic rates from imperfect observations. We apply the IPM 2 in the context of invasive European green crab ( Carcinus maenas ), a species for which individual body size strongly regulates both the observation‐generating process and latent, population dynamics. The IPM 2 facilitates the distinct estimation of green crab size‐structured harvest and natural mortality rates, parameters for which no explicit data is collected and that are unidentifiable in component datasets of the integrated population model. The model represents how the green crab population changes over time, providing the first estimates of size‐structured abundance of this high‐priority species. By forecasting the stable size distribution and equilibrium population size under varying removal efforts, we demonstrate that extremely high levels of removal effort can reduce the equilibrium green crab population size. Yet these high mortality rates also shift the stable size distribution and increase the equilibrium abundance of smaller crabs, since size‐selective removal alters intraspecific interactions. The ecological outcome of this shift in size structure will be variable, as green crab size modulates only some of its interactions with other species. These results highlight the value of the IPM 2 framework for inferring complex population dynamics with information needs that outpace information in individual observational datasets, providing a path forward for accurate assessment of conservation programs.

Keller, Abigail G. [Department of Environment Scie↗

DNA Viral Diversity, Abundance, and Functional Potential Vary across Grassland Soils with a Range of Historical Moisture Regimes

Soil viruses are abundant, but the influence of the environment and climate on soil viruses remains poorly understood. Here, we addressed this gap by comparing the diversity, abundance, lifestyle, and metabolic potential of DNA viruses in three grassland soils with historical differences in average annual precipitation, low in eastern Washington (WA), high in Iowa (IA), and intermediate in Kansas (KS). Bioinformatics analyses were applied to identify a total of 2,631 viral contigs, including 14 complete viral genomes from three deep metagenomes (1 terabase [Tb] each) that were sequenced from bulk soil DNA. An additional three replicate metagenomes (~0.5 Tb each) were obtained from each location for statistical comparisons. Identified viruses were primarily bacteriophages targeting dominant bacterial taxa. Both viral and host diversity were higher in soil with lower precipitation. Viral abundance was also significantly higher in the arid WA location than in IA and KS. More lysogenic markers and fewer clustered regularly interspaced short palindromic repeats (CRISPR) spacer hits were found in WA, reflecting more lysogeny in historically drier soil. More putative auxiliary metabolic genes (AMGs) were also detected in WA than in the historically wetter locations. The AMGs occurring in 18 pathways could potentially contribute to carbon metabolism and energy acquisition in their hosts. Structural equation modeling (SEM) suggested that historical precipitation influenced viral life cycle and selection of AMGs. The observed and predicted relationships between soil viruses and various biotic and abiotic variables have value for predicting viral responses to environmental change.

59 BASIC BIOLOGICAL SCIENCES↗

Optimal limited state variable feedback controllers for linear systems.

Optimized static and dynamic linear system compensator designs with a priori fixed dimensions are discussed. Expressions are derived to determine the parameters of these designs, including the fixed-gain output feedback, the time-varying output feedback, and time-invariant compensation. A relevant deterministic off-line optimization problem is formulated, and the properties of a solution to the problem are analyzed. A unified design philosophy for limited state-variable control problems of essentially deterministic systems is developed. The philosophy incorporates design constraints, a careful selection of performance criteria, and interpretation of the results.

Levine, W. S.↗

Zoom-climb altitude maximization of the F-4C and F-15 aircraft for stratospheric sampling missions

Some predictions indicate that byproducts of aerosol containers may lead to a modification of the ultraviolet-radiation shielding properties of the upper atmosphere. NASA currently monitors atmospheric properties to 70,000 feet using U-2 aircraft. Testing is needed at about 100,000 feet for adequate monitoring of possible aerosol contaminants during the next decade. To study this problem the F-4C and F-15 aircraft were analyzed to determine their maximum altitude ability in zoom-climb maneuvers. These trajectories must satisfy realistic dynamic pressure and Mach number constraints. Maximum altitudes obtained for the F4-C are above 90,000 feet, and for the F-15 above 100,000 feet. Sensitivities of the zoom-climb altitudes were found with respect to several variables including vehicle thrust, initial weight, stratospheric winds and the constraints. A final decision on aircraft selection must be based on mission modification costs and operational considerations balanced against their respective zoom altitude performance capabilities.

Hague, D. S.↗

Preliminary power train design for a state-of-the-art electric vehicle

Power train designs which can be implemented within the current state-of-the-art were identified by means of a review of existing electric vehicles and suitable off-the-shelf components. The affect of various motor/transmission combinations on vehicle range over the SAE J227a schedule D cycle was evaluated. The selected, state-of-the-art power train employs a dc series wound motor, SCR controller, variable speed transmission, regenerative braking, drum brakes and radial ply tires. Vehicle range over the SAE cycle can be extended by approximately 20% by the further development of separately excited, shunt wound DC motors and electrical controllers. Approaches which could improve overall power train efficiency, such as AC motor systems, are identified. However, future emphasis should remain on batteries, tires and lightweight structures if substantial range improvements are to be achieved.

Mighdoll, P.↗

Time transformations and Cowell's method

The precise numerical integration of Cowell's equations of satellite motion is frequently performed with an independent variable s defined by an equation of the form dt = cr to the n-th power ds, where t represents time, r the radial distance from the center of attraction, c is a constant, and n is a parameter. This has been primarily motivated by the 'uniformizing' effects of such a transformation resulting in desirable 'analytic' stepsize control for elliptical orbits. This report discusses the 'proper' choice of the parameter n defining the independent variable s for various types of orbits and perturbation models, and develops a criterion for its selection.

Velez, C. E.↗

A diagnostic model for studying daytime urban air quality trends

A single cell Eulerian photochemical air quality simulation model was developed and validated for selected days of the 1976 St. Louis Regional Air Pollution Study (RAPS) data sets; parameterizations of variables in the model and validation studies using the model are discussed. Good agreement was obtained between measured and modeled concentrations of NO, CO, and NO2 for all days simulated. The maximum concentration of O3 was also predicted well. Predicted species concentrations were relatively insensitive to small variations in CO and NOx emissions and to the concentrations of species which are entrained as the mixed layer rises.

Brewer, D. A.↗

Landsat features for agricultural applications

This paper presents relationships among Landsat MSS bands and selected transformations of them, with emphasis on the Tasseled-Cap Transformation and its Brightness, Greenness variables. It also discusses relationships between reflectance measurements made in the Landsat spectral bands and actual Landsat data. Agronomically oriented analyses of reflectance measurements of wheat throughout a growing season are presented, with a comparison of various green measures, correlation with crop development stage, and examination of the effects of moisture stress. The final example addresses the use of transformed variables in a newly developed approach to forestry change detection.

Malila, W. A.↗

Pre-design study for a modern four-bladed rotor for the Rotor System Research Aircraft (RSRA)

Various candidate rotor systems were compared in an effort to select a modern four-bladed rotor for the RSRA. The YAH-64 rotor system was chosen as the candidate rotor system for further development for the RSRA. The process used to select the rotor system, studies conducted to mate the rotor with the RSRA and provide parametric variability, and the development plan which would be used to implement these studies are presented. Drawings are included.

Hughes, C. W.↗

The Ne-21 production rate in stony meteorites estimated from Be-10 and other radionuclides

The Be-10 contents of 28 stony meteorites with known Ne-21 contents range from 0.97 to 23 dpm/kg and give an average Ne-21 production rate (P sub 21) of (0.28 + or - 0.02) x 10 to the -8th cu cm STP/g-My for shielding conditions corresponding to Ne-22/Ne-21 = 1.114 in an H-chondrite. This P sub 21 (Be) agrees with others' P sub 21 based on Na-22, Kr-81, and Mn-53, but not an Al-26. Temporal variations in the cosmic ray flux do not explain the disagreement satisfactorily; major errors in the radionuclide half-lives are not indicated. The discrepancy seems rooted in the data selection and the difficulties of making accurate corrections for shielding, chemical composition and other sources of variability.

Moniot, R. K.↗

Locations and time histories of five 1979 gamma-ray bursts

The locations and time histories of five gamma-ray bursts that occurred between 1979 March 7 and March 31 were studied. The error box for GB 790325 has a typical dimension of about 1.5 arcmin. The other localizations, while not precise enough for thorough optical examination, contribute to distribution studies and allow radio and X-ray observations, catalog searches, and other archival work. A search through selected catalogs did reveal one object, the star FY Aql (cataloged as a Mira-type variable but probably a dwarf nova) inside one of the gamma-ray burst boxes. Given the parameters of this particular search, the probability of at least one chance association is 0.03. Both recent and archival optical examinations of some of the error boxes were carried out, and no indications of any actual physical associations were seen. One event, GB 790331, had an interesting spectral behavior, in that the leading edges of the two main peaks within the burst had harder spectra than the remainder of the event.

Laros, J. G.↗

A midlatitude interhemispheric model of the O(+) (2P) airglow emission at 7320 A

The results are reported of the first interhemispheric model of the 7320 A airglow covering mid- and low latitudes, at all longitudes. A comprehensive model of the ionosphere and thermosphere is used to compute volume emission rates as a function of altitude, latitude, longitude, and local time. Selected results are shown here to illustrate the computational capability. In particular, the diurnal and seasonal variability and interhemispheric coupling of conjugate photoelectrons are discussed. The model is particularly well suited for airglow studies, and provides a valuable tool for the comparison, interpretation, and planning of spectroscopic observations made from orbiting platforms for all significant thermospheric emissions.

Torr, Marsha R.↗

Airborne CO2 coherent lidar measurements of cloud backscatter and opacity over the ocean surface

The use of an airborne CO2 lidar to obtain cloud backscatter and extinction data at a thermal infrared wavelength is described. The extinction in this spectral region is proportional to the cloud liquid water content. The use of coherent detection results in high sensitivity and narrow field of view, the latter property greatly reducing multiple-scattering effects. Backscatter measurements in absolute units are obtained through a hard target calibration methodology. For clouds of low to moderate optical thickness at the lidar wavelength, both geometric thickness and optical thickness can be measured. The sea surface reflectance signal is used to obtain estimates of the cloud optical thickness. The utility of this technique results from studies that indicate that the spatial scale of variability of the sea surface reflectance is generally large compared with that of cloud optical thickness. Selected results are presented from data taken during flights over the Pacific Ocean.

Menzies, Robert T.↗

Aero-Structural Interaction, Analysis, and Shape Sensitivity

A multidisciplinary sensitivity analysis technique that has been shown to be independent of step-size selection is examined further. The accuracy of this step-size independent technique, which uses complex variables for determining sensitivity derivatives, has been previously established. The primary focus of this work is to validate the aero-structural analysis procedure currently being used. This validation consists of comparing computed and experimental data obtained for an Aeroelastic Research Wing (ARW-2). Since the aero-structural analysis procedure has the complex variable modifications already included into the software, sensitivity derivatives can automatically be computed. Other than for design purposes, sensitivity derivatives can be used for predicting the solution at nearby conditions. The use of sensitivity derivatives for predicting the aero-structural characteristics of this configuration is demonstrated.

Newman, James C., III↗

Fabrication of Extremely Short Length Fiber Bragg Gratings for Sensor Applications

A new technique and a physical model for writing extremely short length Bragg gratings in optical fibers have been developed. The model describes the effects of diffraction on the spatial spectra and therefore, the wavelength spectra of the Bragg gratings. Using an interferometric technique and a variable aperture, short gratings of various lengths and center wavelengths were written in optical fibers. By selecting the related parameters, the Bragg gratings with typical length of several hundred microns and bandwidth of several nanometers can be obtained. These short gratings can be apodized with selected diffraction patterns and hence their broadband spectra have a well-defined bell shape. They are suitable for use as miniaturized distributed strain sensors, which have broad applications to aerospace research and industry as well.

Wu, Meng-Chou↗