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Mineralogical and chemical characterization of iron-, manganese-, and copper-containing synthetic hydroxyapatites

The National Aeronautics and Space Administration's (NASA) Advanced Life Support (ALS) Program is evaluating the use of Fe-, Mn-, and Cu-containing synthetic hydroxyapatite (SHA) as a slow release fertilizer for crops that might be grown on the International Space Station or at Lunar and Martian outposts. Separate Fe-, Mn-, and Cu-containing SHA materials along with a transition-metal free SHA (pure-SHA) were synthesized using a precipitation method. Chemical and mineralogical analyses determined if and how Fe, Mn, and Cu were incorporated into the SHA structure. X-ray diffraction (XRD), Rietveld refinement, and transmission electron microscopy (TEM) confirmed that SHA materials with the apatite structure were produced. Chemical analyses indicated that the metal containing SHA materials were deficient in Ca relative to pure-SHA. The shift in the infrared PO4-mu 3 vibrations, smaller unit cell parameters, smaller particle size, and greater structural strain for Fe-, Mn-, and Cu-containing SHA compared with pure-SHA suggested that Fe, Mn, and Cu were incorporated into SHA structure. Rietveld analyses revealed that Fe, Mn, and Cu substituted into the Ca2 site of SHA. An Fe-rich phase was detected by TEM analyses and backscattered electron microscopy in the Fe-containing SHA material with the greatest Fe content. The substitution of metals into SHA suggests that metal-SHA materials are potential slow-release sources of micronutrients for plant uptake in addition to Ca and P.

NASA Center JSC↗

Modified Penning-Malmberg Trap for Storing Antiprotons

A modified Penning-Malmberg trap that could store a small cloud of antiprotons for a relatively long time (weeks) has been developed. This trap is intended for use in research on the feasibility of contemplated future matter/antimatter-annihilation systems as propulsion sources for spacecraft on long missions. This trap is also of interest in its own right as a means of storing and manipulating antiprotons for terrestrial scientific experimentation. The use of Penning-Malmberg traps to store antiprotons is not new. What is new here is the modified trap design, which utilizes state-of-the-art radiofrequency (RF) techniques, including ones that, heretofore, have been used in radio-communication applications but not in iontrap applications. A basic Penning-Malmberg trap includes an evacuated round tube that contains or is surrounded by three or more collinear tube electrodes. A steady axial magnetic field that reaches a maximum at the geometric center of the tube is applied by an external source, and DC bias voltages that give rise to an electrostatic potential that reaches a minimum at the center are applied to the electrodes. The combination of electric and magnetic fields confines the charged particles (ions or electrons) for which it was designed to a prolate spheroidal central region. However, geometric misalignments and the diffusive cooling process prevent the steady fields of a basic Penning- Malmberg trap from confining the particles indefinitely. In the modified Penning-Malmberg trap, the loss of antiprotons is reduced or eliminated by use of a "rotating-wall" RF stabilization scheme that also heats the antiproton cloud to minimize loss by matter/antimatter annihilation. The scheme involves the superposition of a quadrupole electric field that rotates about the cylindrical axis at a suitably chosen radio frequency. The modified Penning-Malmberg trap (see Figure 1) includes several collinear sets of electrodes inside a tubular vacuum chamber. Each set comprises either a single metal tube or else a tube that is segmented into four electrodes that subtend equal angles about the cylindrical axis. The output of an RF signal generator is fed through a 90 hybrid coupler and then through two baluns to generate four replicas of the signal at relative phase shifts of 0 , 90 , 180 , and 270 (see Figure 2). These signal replicas are fed through 6-dB directional couplers, then via coaxial cables to the vacuum chamber. The signal is then routed to a phase cancellation network, which filters out the drive signal with the difference representing the plasma interaction. Inside the vacuum chamber, twisted-pair wires feed the signals from the coaxial cables to the four electrodes of each segmented electrode tube.

Sims, William H.↗

Static performance investigation of a skewed-throat multiaxis thrust-vectoring nozzle concept

The static performance of a jet exhaust nozzle which achieves multiaxis thrust vectoring by physically skewing the geometric throat has been characterized in the static test facility of the 16-Foot Transonic Tunnel at NASA Langley Research Center. The nozzle has an asymmetric internal geometry defined by four surfaces: a convergent-divergent upper surface with its ridge perpendicular to the nozzle centerline, a convergent-divergent lower surface with its ridge skewed relative to the nozzle centerline, an outwardly deflected sidewall, and a straight sidewall. The primary goal of the concept is to provide efficient yaw thrust vectoring by forcing the sonic plane (nozzle throat) to form at a yaw angle defined by the skewed ridge of the lower surface contour. A secondary goal is to provide multiaxis thrust vectoring by combining the skewed-throat yaw-vectoring concept with upper and lower pitch flap deflections. The geometric parameters varied in this investigation included lower surface ridge skew angle, nozzle expansion ratio (divergence angle), aspect ratio, pitch flap deflection angle, and sidewall deflection angle. Nozzle pressure ratio was varied from 2 to a high of 11.5 for some configurations. The results of the investigation indicate that efficient, substantial multiaxis thrust vectoring was achieved by the skewed-throat nozzle concept. However, certain control surface deflections destabilized the internal flow field, which resulted in substantial shifts in the position and orientation of the sonic plane and had an adverse effect on thrust-vectoring and weight flow characteristics. By increasing the expansion ratio, the location of the sonic plane was stabilized. The asymmetric design resulted in interdependent pitch and yaw thrust vectoring as well as nonzero thrust-vector angles with undeflected control surfaces. By skewing the ridges of both the upper and lower surface contours, the interdependency between pitch and yaw thrust vectoring may be eliminated and the location of the sonic plane may be further stabilized.

Wing, David J.↗

An evaluation of three-dimensional sensors for the extravehicular activity helper/retreiver

The Extravehicular Activity Retriever/Helper (EVAHR) is a robotic device currently under development at the NASA Johnson Space Center that is designed to fetch objects or to assist in retrieving an astronaut who may have become inadvertently de-tethered. The EVAHR will be required to exhibit a high degree of intelligent autonomous operation and will base much of its reasoning upon information obtained from one or more three-dimensional sensors that it will carry and control. At the highest level of visual cognition and reasoning, the EVAHR will be required to detect objects, recognize them, and estimate their spatial orientation and location. The recognition phase and estimation of spatial pose will depend on the ability of the vision system to reliably extract geometric features of the objects such as whether the surface topologies observed are planar or curved and the spatial relationships between the component surfaces. In order to achieve these tasks, accurate sensing of the operational environment and objects in the environment will therefore be critical. The qualitative and quantitative results of empirical studies of three sensors that are capable of providing three-dimensional information to the EVAHR, but using completely different hardware approaches are documented. The first of these devices is a phase shift laser with an effective operating range (ambiguity interval) of approximately 15 meters. The second sensor is a laser triangulation system designed to operate at much closer range and to provide higher resolution images. The third sensor is a dual camera stereo imaging system from which range images can also be obtained. The remainder of the report characterizes the strengths and weaknesses of each of these systems relative to quality of data extracted and how different object characteristics affect sensor operation.

Magee, Michael↗

Ethylene-associated phase change from juvenile to mature phenotype of daylily (Hemerocallis) in vitro

Hemerocallis plantlets maintained in vitro for extended periods of time in tightly closed culture vessels frequently show a phenotype, albeit on a miniaturized scale, typical of more mature, field-grown plants. The positive relationship of elevated ethylene in the headspace of such vessels to the phase shift from juvenile to mature form is established. Rigorous restriction in air exchange with the external environment by means of silicone grease seals hastens the phase change and improves uniformity of response. Although some plantlets may take longer to accumulate enough ethylene in sealed jars to undergo change, added ethylene and ethylene-releasing agents promote it. Ethylene adsorbants (e.g. mercuric perchlorate) block the shift of juvenile to mature form. Critical ambient ethylene level for the shift is ca 1 microliter l-1. Levels up to 1000 microliters l-1 do not hasten the response but are not toxic. The phase change is fully reversible when air exchange permits ethylene to drop below 1 microliter l-1. At least 1 microliter l-1 ethylene is required to sustain the mature phenotype. The ethylene synthesis inhibitor aminoethoxyvinylglycine (AVG) prevents the phase change, while the ethylene biosynthesis intermediate 1-aminocyclopropanecarboxylic acid (ACC) improves it. KOH, as a CO2 absorbent, does not prevent the phase change. Histology sections demonstrate subtle changes in the form of shoot tips of plantlets undergoing phase change.

NASA Discipline Plant Biology↗

Lessons Learned on Operating and Preparing Operations for a Technology Mission from the Perspective of the Earth Observing-1 Mission

The New Millennium Program's first Earth-observing mission (EO-1) is a technology validation mission. It is managed by the NASA Goddard Space Flight Center in Greenbelt, Maryland and is scheduled for launch in the summer of 2000. The purpose of this mission is to flight-validate revolutionary technologies that will contribute to the reduction of cost and increase of capabilities for future land imaging missions. In the EO-1 mission, there are five instrument, five spacecraft, and three supporting technologies to flight-validate during a year of operations. EO-1 operations and the accompanying ground system were intended to be simple in order to maintain low operational costs. For purposes of formulating operations, it was initially modeled as a small science mission. However, it quickly evolved into a more complex mission due to the difficulties in effectively integrating all of the validation plans of the individual technologies. As a consequence, more operational support was required to confidently complete the on-orbit validation of the new technologies. This paper will outline the issues and lessons learned applicable to future technology validation missions. Examples of some of these include the following: (1) operational complexity encountered in integrating all of the validation plans into a coherent operational plan, (2) initial desire to run single shift operations subsequently growing to 6 "around-the-clock" operations, (3) managing changes in the technologies that ultimately affected operations, (4) necessity for better team communications within the project to offset the effects of change on the Ground System Developers, Operations Engineers, Integration and Test Engineers, S/C Subsystem Engineers, and Scientists, and (5) the need for a more experienced Flight Operations Team to achieve the necessary operational flexibility. The discussion will conclude by providing several cost comparisons for developing operations from previous missions to EO-1 and discuss some details that might be done differently for future technology validation missions.

Mandl, Dan↗

Drop Size Distribution Measurements Supporting the NASA Global Precipitation Measurement Mission: Infrastructure and Preliminary Results

Global Precipitation Measurement Mission (GPM) retrieval algorithm validation requires datasets that characterize the 4-D structure, variability, and correlation properties of hydrometeor particle size distributions (PSD) and accumulations over satellite fields of view (5 -- 50 km). Key to this process is the combined use of disdrometer and polarimetric radar platforms. Here the disdrometer measurements serve as a reference for up-scaling dual-polarimetric radar observations of the PSD to the much larger volumetric sampling domain of the radar. The PSD observations thus derived provide a much larger data set for assessing DSD variability, and satellite-based precipitation retrieval algorithm assumptions, in all three spatial dimensions for a range of storm types and seasons. As one component of this effort, the GPM Ground Validation program recently acquired five 3rd generation 2D Video disdrometers as part of its Disdrometer and Radar Observations of Precipitation Facility (DROP), currently hosted in northern Alabama by the NASA Marshall Space Flight Center and the University of Alabama in Huntsville. These next-generation 2DVDs were operated and evaluated in different phases of data collection under the scanning domain of the UAH ARMOR C-band dual-polarimetric radar. During this period approximately 7500 minutes of PSD data were collected and processed to create gamma size distribution parameters using a truncated method of moments approach. After creating the gamma parameter datasets the DSDs were then used as input to T-matrix code for computation of polarimetric radar moments at C-band. The combined dataset was then analyzed with two basic objectives in mind: 1) the investigation of seasonal variability in the rain PSD parameters as observed by the 2DVDs; 2) the use of combined polarimetric moments and observed gamma distribution parameters in a functional form to retrieve PSD parameters in 4-D using the ARMOR radar for precipitation occurring in different seasons and for different rain system types. Preliminary results suggest that seasonal variations in the DSD parameters do occur, but are most pronounced when comparing tropical PSDs to either winter or summer convective precipitation. For example the previously documented shift to relatively smaller drop diameters in higher number concentrations for equivalent rain rate bins was observed in tropical storm rainbands occurring over Huntsville. On a more inter seasonal basis empirical fits between parameters such as D0 and ZDR do not appear to exhibit robust seasonal biases- i.e., one fit seems to work for all seasons within acceptable standard error (O[10%]) for estimates of D0. In polarimetric retrievals of the vertical variability in PSD (rain layer) for a tropical rainband we find that the Do varies with height when partitioned by specified precipitation categories (e.g., convective or stratiform, heavy and light stratiform etc.) but this variation is of order 10-20% and is smaller than the difference in D0 observed between the basic delineation of convective and stratiform precipitation types. Currently we are expanding our analysis of the vertical structure of the PSD to include several seasonally and/or dynamically-different storm system types (e.g., winter convection and stratiform events; summer mid-latitude convective etc.) sampled by ARMOR. The study will present the results of our combined analyses.

Petersen, Walter A.↗

Cell prestress. II. Contribution of microtubules

The tensegrity model hypothesizes that cytoskeleton-based microtubules (MTs) carry compression as they balance a portion of cell contractile stress. To test this hypothesis, we used traction force microscopy to measure traction at the interface of adhering human airway smooth muscle cells and a flexible polyacrylamide gel substrate. The prediction is that if MTs balance a portion of contractile stress, then, upon their disruption, the portion of stress balanced by MTs would shift to the substrate, thereby causing an increase in traction. Measurements were done first in maximally activated cells (10 microM histamine) and then again after MTs had been disrupted (1 microM colchicine). We found that after disruption of MTs, traction increased on average by approximately 13%. Because in activated cells colchicine induced neither an increase in intracellular Ca(2+) nor an increase in myosin light chain phosphorylation as shown previously, we concluded that the observed increase in traction was a result of load shift from MTs to the substrate. In addition, energy stored in the flexible substrate was calculated as work done by traction on the deformation of the substrate. This result was then utilized in an energetic analysis. We assumed that cytoskeleton-based MTs are slender elastic rods supported laterally by intermediate filaments and that MTs buckle as the cell contracts. Using the post-buckling equilibrium theory of Euler struts, we found that energy stored during buckling of MTs was quantitatively consistent with the measured increase in substrate energy after disruption of MTs. This is further evidence supporting the idea that MTs are intracellular compression-bearing elements.

Non-NASA Center↗

"Reagentless" flow injection determination of ammonia and urea using membrane separation and solid phase basification

Flow injection analysis instrumentation and methodology for the determination of ammonia and ammonium ions in an aqueous solution are described. Using in-line solid phase basification beds containing crystalline media. the speciation of ammoniacal nitrogen is shifted toward the un-ionized form. which diffuses in the gas phase across a hydrophobic microporous hollow fiber membrane into a pure-water-containing analytical stream. The two streams flow in a countercurrent configuration on opposite sides of the membrane. The neutral pH of the analytical stream promotes the formation of ammonium cations, which are detected using specific conductance. The methodology provides a lower limit of detection of 10 microgram/L and a dynamic concentration range spanning three orders of magnitude using a 315-microliters sample injection volume. Using immobilized urease to enzymatically promote the hydrolysis of urea to produce ammonia and carbon dioxide, the technique has been extended to the determination of urea.

NASA Center JSC↗

Ion Beam Characterization of a NEXT Multi-Thruster Array Plume

Three operational, engineering model, 7-kW ion thrusters and one instrumented, dormant thruster were installed in a cluster array in a large vacuum facility at NASA Glenn Research Center. A series of engineering demonstration tests were performed to evaluate the system performance impacts of operating various multiple-thruster configurations in an array. A suite of diagnostics was installed to investigate multiple-thruster operation impact on thruster performance and life, thermal interactions, and alternative system modes and architectures. The ion beam characterization included measuring ion current density profiles and ion energy distribution with Faraday probes and retarding potential analyzers, respectively. This report focuses on the ion beam characterization during single thruster operation, multiple thruster operation, various neutralizer configurations, and thruster gimbal articulation. Comparison of beam profiles collected during single and multiple thruster operation demonstrated the utility of superimposing single engine beam profiles to predict multi-thruster beam profiles. High energy ions were detected in the region 45 off the thruster axis, independent of thruster power, number of operating thrusters, and facility background pressure, which indicated that the most probable ion energy was not effected by multiple-thruster operation. There were no significant changes to the beam profiles collected during alternate thruster-neutralizer configurations, therefore supporting the viability of alternative system configuration options. Articulation of one thruster shifted its beam profile, whereas the beam profile of a stationary thruster nearby did not change, indicating there were no beam interactions which was consistent with the behavior of a collisionless beam expansion.

Pencil, Eric J.↗

Adjusting a Full Body Model to Mitigate Inverse Kinematics Artifacts in Opensim

BACKGROUND: In support of Vibration Isolation and Stabilization (VIS) system development for Human Health Countermeasures (HHC) exercise systems in space, such as the European Enhanced Exploration Exercise Device (E4D) [1], dynamic quantities required to model the response of a proposed VIS while considering the effect of VIS motion on the forces between the human and VIS platform were obtained using motion capture data [2]. On occasion, large-amplitude oscillatory spikes were found in the subject’s linear and angular momentum derivatives, affecting analyses that depend on forces and moments derived from motion capture. The purpose of this investigation was to identify causes of these artifacts and techniques for their resolution. METHODS AND RESULTS: To obtain the required human dynamic quantities to drive the VIS simulation, motion capture data was collected containing recorded trajectories of passive retroreflective markers on body landmarks of an exercising subject. Since the full body Rajagopal model [3] was originally used to enhance gait analysis, upper body joints did not require large Ranges of Motion (ROM). We thus modified the Rajagopal model [4, 5] to allow it to be used for upper body intensive exercises like those common to the E4D. OpenSim Inverse Kinematics (IK) [6] was performed using these scaled subject models to generate the joint angles throughout the exercise while minimizing marker error. At times, the arms were observed to ‘snap’ from one configuration to another, causing spike artifacts. Following IK, a custom OpenSim plugin [7] was used to determine the required dynamic quantities including the rates of change of the linear and angular momenta of the human. Since motion capture is recorded at a larger time step than required by the VIS simulation, the human center of mass location was fit with splines and a second derivative taken to obtain the momentum derivative, allowing the VIS simulation to maintain conservation of momentum when appropriate. In a few cases during this stage, artifacts much larger than the expected noise of the second derivatives were introduced. Investigation of cases containing artifacts revealed several modifications that could be made to the OpenSim model to improve IK results. Since OpenSim models use Euler angles and rotation sequences, ‘gimbal lock’ would be encountered in the arms when raised 90 degrees to the side (e.g., T-pose, some hang clean exercise, etc.). This was resolved by reorienting the horizontal axes at the shoulder joint by 45 degrees, placing ‘gimbal lock’ outside common arm ROM, with the arm ROMs adjusted following this change. Elbow and wrist ROMs could also be adjusted to allow realistic motion while at the same time limiting the likelihood of unrealistic orientations. On occasion, the arms flipped backwards when raised above the head. This was prevented by using medial elbow markers in scaling and IK. When medial markers were not available, the acromial joint location in the unscaled model was shifted before model scaling to better align the arm with available markers. Lastly, artifacts which became apparent after taking the second derivatives of spline-fit data were found to occur in cases when the pelvis rotation limit prevented the full range of motion of an exercise. These issues were resolved by unclamping the pelvis rotation limit. Through this investigation, an understanding of conditions leading to IK artifacts was acquired allowing the automation of artifact detection. These artifact detection and mitigation techniques can be applied toward modeling of upper body motions in aerospace and other fields for improved IK results.

C A Bell↗

Activation of a chloroplast type of fructose bisphosphatase from Chlamydomonas reinhardtii by light-mediated agents

A chloroplast type of fructose-1,6-bisphosphatase, a central regulatory enzyme of photosynthetic carbon metabolism, has been partially purified from Chlamydomonas reinhardtii. Unlike its counterpart from spinach chloroplasts, the algal FBPase showed a strict requirement for a dithiol reductant irrespective of Mg2+ concentration. The enzymes from the two sources resembled each other immunologically, in subunit molecular mass and response to pH. In the presence of dithiothreitol, the pH optimum for both the algal and spinach enzymes shifted from 8.5 to a more physiologic value of 8.0 as the Mg2+ concentration was increased from 1 to 16 mM. At 1 mM Mg2+, a concentration estimated to be close to physiological, the Chlamydomonas FBPase was active only in the presence of reduced thioredoxin and was most active with Chlamydomonas thioredoxin f. Under these conditions, the enzyme showed a pH optimum of 8.0. The data suggest that the Chlamydomonas enzyme resembles its spinach counterpart in most respects, but it has a stricter requirement for reduction and less strict reductant specificity. A comparison of the properties of the FBPases from Chlamydomonas and spinach will be helpful for elucidating the mechanism of the reductive activation of this enzyme.

NASA Discipline Exobiology↗

Development of Weighout Process and Evaluations for Underwater Partial Gravity EVA Simulations

For the upcoming Artemis lunar missions, astronauts will needto train in a spacesuit where partial gravity can be simulated such as NASA Neutral Buoyancy Lab (NBL). At the NBL, dive weights and foam can be added around the spacesuit to attaina satisfactory center of buoyancy (CB) and center of gravity (CG) location to simulate the lunar gravity (1/6thG) effects. If CG and CB are not co-located properly, incorrect righting moments can be introduced, and both simulation quality and EVA task performance can be impaired. Based on the findings from the initial testingusing xEMU spacesuits, it was observed that the weigh-out method (i.e., determination of the weights and foam quantities and position) needed further development to improve the simulation quality, especially for the subjects who experienced excessive instability. This paper aims to present the on-going effort to improve the weigh-out process for enabling NBL lunar EVA simulations. For this effort, a human-suit model was created to use suit CAD and 3D human body scansto estimate both CB and CG location for each suited subject. NBL weigh-out testing was performed to characterize the effects of CG and CB positioning, in which the 3D human-suit model was used to determine optimal weigh-out combinations of weights and foam. Postural, balance, and subjective feedback were gathered for each weigh-out configuration. The results indicated that, as the CB was shifted higher and the CB and CG located closer to each other, the subject tended to be more stable and their EVA performance improved. A high CB location was then prioritized across 4 additional subjects in both small and large size spacesuits. When compared to the initial xEMU test series, improved performance was observed across all subjects as the CB moved higher and aligned closer to the system CG.

Seyed Pouyan Sabahi↗

Acridine orange staining reaction as an index of physiological activity in Escherichia coli

The assumption that the acridine orange (AO) color reaction may be used as an index of physiological activity was investigated in laboratory grown Escherichia coli. Spectrofluorometric observations of purified nucleic acids, ribosomes and the microscopic color of bacteriophage-infected cells stained with AO confirmed the theory that single-stranded nucleic acids emit orange to red fluorescence while those that are double-stranded fluoresce green in vivo. Bacteria growing actively in a rich medium could be distinguished from cells in stationary phase by the AO reaction. Cells from log phase appeared red, whereas those in stationary phase were green. However, this differentiation was not seen when the bacteria were grown in a minimal medium or when a variation of the staining method was used. Also, shifting bacteria in stationary phase to starvation conditions rapidly changed their AO staining reaction. Boiling and exposure to lethal concentrations of azide and formalin resulted in stationary-phase cells that appeared red after staining but bacteria killed with chlorine remained green. These findings indicate that the AO staining reaction may be suggestive of physiological activity under defined conditions. However, variables in staining and fixation procedures as well as uncertainties associated with mixed bacterial populations in environmental samples may produce results that are not consistent with the classical interpretation of this reaction. The importance of validating the putative physiological implications of this staining reaction is stressed.

NASA Discipline Number 04-10↗

Evaluation of Cloud Physical Properties of ECMWF Analysis and Re-Analysis (ERA-40 and ERA Interim) against CERES Tropical Deep Convective Cloud Object Observations

This study presents an approach that converts the vertical profiles of grid-averaged cloud properties from large-scale models to probability density functions (pdfs) of subgrid-cell cloud physical properties measured at satellite footprints. Cloud physical and radiative properties, rather than just cloud and precipitation occurrences, of assimilated cloud systems by the European Center for Medium-range Weather Forecasts (ECMWF) operational analysis (EOA) and ECMWF Re-Analyses (ERA-40 and ERA Interim) are validated against those obtained from Earth Observing System satellite cloud object data for January-August 1998 and March 2000 periods. These properties include ice water path (IWP), cloud-top height and temperature, cloud optical depth and solar and infrared radiative fluxes. Each cloud object, a contiguous region with similar cloud physical properties, is temporally and spatially matched with EOA and ERA-40 data. Results indicate that most pdfs of EOA and ERA-40 cloud physical and radiative properties agree with those of satellite observations of the tropical deep convective cloud-object type for the January-August 1998 period. There are, however, significant discrepancies in selected ranges of the cloud property pdfs such as the upper range of EOA cloud top height. A major discrepancy is that the dependence of the pdfs on the cloud object size for both EOA and ERA-40 is not as strong as in the observations. Modifications to the cloud parameterization in ECMWF that occurred in October 1999 eliminate the clouds near the tropopause but shift power of the pdf to lower cloud-top heights and greatly reduce the ranges of IWP and cloud optical depth pdfs. These features persist in ERA-40 due to the use of the same cloud parameterizations. The downgrade of data assimilation technique and the lack of snow water content information in ERA-40, not the coarser horizontal grid resolution, are also responsible for the disagreements with observed pdfs of cloud physical properties although the detection rates of cloud object occurrence are improved for small size categories. A possible improvement to the convective parameterization is to introduce a stronger dependence of updraft penetration heights with grid-cell dynamics. These conclusions will be rechecked using the ERA Interim data, due to recent changes in the ECMWF convective parameterization (Bechtold et al. 2004, 2008). Results from the ERA Interim will be presented at the meeting.

Xu, Kuan-Man↗

Langley's views on NEMS

The views of the Langley Research Center regarding the NASA Equipment Management System (EMS) are discussed. One of Langley's greatest concerns is with the reconciliation between NEMS and the General Ledger. Langley's accounting system tracks cost data to the penny level. NEMS deals in whole dollar amounts. Therefore, Langley has no way of reconciling the two. The only approach that is acceptable to Langley, unless requirements for reconciliation are changed, is for the NEMS files and the reports involved in the process be at the penny level. All other NEMS reports can remain whole dollars. Also to reconcile, Langley needs data to show the difference between the previous cost and the new cost for the month. On an input record, the adjustment amount is added to the cost and recorded as total amount. The adjusted cost is not captured. In order to establish a control between the prior months and the current month, a new field needs to be added to capture the adjusted cost (debits And credits). Langley has not reconciled the Equipment account with the General Ledger since February 1984. Problems with NEMS regular production runs cause concern. Production at Langley is run on the second and/or third shift. If a run(s) terminates and/or abends in a particular module, Langley must wait until the next day to resolve NEMS problems after consultation with Headquarters personnel. For a successful installation, Langley must have a good data base to convert to NEMS and users and the data processing staff must work together.

George, J. W.↗

Development of Weigh-out Process and Evaluations for Underwater Partial Gravity Simulations

For the upcoming Artemis lunar missions, astronauts will need to train in a spacesuit where partial gravity can be simulated such as the NASA Neutral Buoyancy Lab (NBL). At the NBL, dive weights and foam can be added around the spacesuit to attain a satisfactory center of buoyancy (CB) and center of gravity (CG) location to simulate the lunar gravity (1/6thG) effects. If CG and CB are not co-located properly, incorrect righting moments can be introduced, and both simulation quality and EVA task performance can be impaired. Based on the findings from the initial testing using xEMU spacesuits, it was observed that the weigh-out method (i.e., determination of the weights and foam quantities and position) needed further development to improve the simulation quality, especially for the subjects who experienced excessive instability. This paper aims to present the on-going effort to improve the weigh-out process for enabling NBL lunar EVA simulations. For this effort, a human-suit model was created to use suit CAD and 3D human body scans to estimate both CB and CG location for each suited subject. NBL weigh-out testing was performed to characterize the effects of CG and CB positioning, in which the 3D human-suit model was used to determine optimal weigh-out combinations of weights and foam. Postural, balance, and subjective feedback were gathered for each weigh-out configuration. The results indicated that, as the CB was shifted higher and the CB and CG located closer to each other, the subject tended to be more stable and their EVA performance improved. A high CB location was then prioritized across 4 additional subjects in both small and large size spacesuits. When compared to the initial xEMU test series, improved performance was observed across all subjects as the CB moved higher and aligned closer to the system CG.

Pouyan Sabahi↗

Development of Weigh-out Process and Evaluations for Underwater Partial Gravity Simulations

For the upcoming Artemis lunar missions, astronauts will need to train in a spacesuit where partial gravity can be simulated such as the NASA Neutral Buoyancy Lab (NBL). At the NBL, dive weights and foam can be added around the spacesuit to attain a satisfactory center of buoyancy (CB) and center of gravity (CG) location to simulate the lunar gravity (1/6thG) effects. If CG and CB are not co-located properly, incorrect righting moments can be introduced, and both simulation quality and EVA task performance can be impaired. Based on the findings from the initial testing using xEMU spacesuits, it was observed that the weigh-out method (i.e., determination of the weights and foam quantities and position) needed further development to improve the simulation quality, especially for the subjects who experienced excessive instability. This paper aims to present the on-going effort to improve the weigh-out process for enabling NBL lunar EVA simulations. For this effort, a human-suit model was created to use suit CAD and 3D human body scans to estimate both CB and CG location for each suited subject. NBL weigh-out testing was performed to characterize the effects of CG and CB positioning, in which the 3D human-suit model was used to determine optimal weigh-out combinations of weights and foam. Postural, balance, and subjective feedback were gathered for each weigh-out configuration. The results indicated that, as the CB was shifted higher and the CB and CG located closer to each other, the subject tended to be more stable and their EVA performance improved. A high CB location was then prioritized across 4 additional subjects in both small and large size spacesuits. When compared to the initial xEMU test series, improved performance was observed across all subjects as the CB moved higher and aligned closer to the system CG.

Pouyan Sabahi↗