Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “NEUTRALIZATION”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 973 records · Page 54
All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

GAL08, an Uncultivated Group of Acidobacteria, Is a Dominant Bacterial Clade in a Neutral Hot Spring

GAL08 are bacteria belonging to an uncultivated phylogenetic cluster within the phylum Acidobacteria . We detected a natural population of the GAL08 clade in sediment from a pH-neutral hot spring located in British Columbia, Canada. To shed light on the abundance and genomic potential of this clade, we collected and analyzed hot spring sediment samples over a temperature range of 24.2–79.8°C. Illumina sequencing of 16S rRNA gene amplicons and qPCR using a primer set developed specifically to detect the GAL08 16S rRNA gene revealed that absolute and relative abundances of GAL08 peaked at 65°C along three temperature gradients. Analysis of sediment collected over multiple years and locations revealed that the GAL08 group was consistently a dominant clade, comprising up to 29.2% of the microbial community based on relative read abundance and up to 4.7 × 10 5 16S rRNA gene copy numbers per gram of sediment based on qPCR. Using a medium quality threshold, 25 single amplified genomes (SAGs) representing these bacteria were generated from samples taken at 65 and 77°C, and seven metagenome-assembled genomes (MAGs) were reconstructed from samples collected at 45–77°C. Based on average nucleotide identity (ANI), these SAGs and MAGs represented three separate species, with an estimated average genome size of 3.17 Mb and GC content of 62.8%. Phylogenetic trees constructed from 16S rRNA gene sequences and a set of 56 concatenated phylogenetic marker genes both placed the three GAL08 bacteria as a distinct subgroup of the phylum Acidobacteria , representing a candidate order ( Ca. Frugalibacteriales) within the class Blastocatellia. Metabolic reconstructions from genome data predicted a heterotrophic metabolism, with potential capability for aerobic respiration, as well as incomplete denitrification and fermentation. In laboratory cultivation efforts, GAL08 counts based on qPCR declined rapidly under atmospheric levels of oxygen but increased slightly at 1% (v/v) O 2 , suggesting a microaerophilic lifestyle.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Structures of HIV-1 Neutralizing Antibody 10E8 Delineate the Mechanistic Basis of Its Multi-Peak Behavior on Size-Exclusion Chromatography

Antibody 10E8 is capable of effectively neutralizing HIV through its recognition of the membrane-proximal external region (MPER), and a suitably optimized version of 10E8 might have utility in HIV therapy and prophylaxis. However, 10E8 displays a three-peak profile on size-exclusion chromatography (SEC), complicating its manufacture. Here we show cis-trans conformational isomerization of the Tyr-Pro-Pro (YPP) motif in the heavy chain 3rd complementarity-determining region (CDR H3) of antibody 10E8 to be the mechanistic basis of its multipeak behavior. We observed 10E8 to undergo slow conformational isomerization and delineate a mechanistic explanation for effective comodifiers that were able to resolve its SEC heterogeneity and to allow an evaluation of the critical quality attribute of aggregation. We determined crystal structures of single and double alanine mutants of a key di-proline motif and of a light chain variant, revealing alternative conformations of the CDR H3. We also replicated both multi-peak and delayed SEC behavior with MPER-antibodies 4E10 and VRC42, by introducing a Tyr-Pro (YP) motif into their CDR H3s. Our results show how a conformationally dynamic CDR H3 can provide the requisite structural plasticity needed for a highly hydrophobic paratope to recognize its membrane-proximal epitope.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2023

Cradle-to-Grave Lifecycle Analysis of Greenhouse Gas Emissions of Light-Duty Passenger Vehicles in China: Towards a Carbon-Neutral Future

Vehicle electrification is considered a pathway for on-road transportation decarbonization in China. Different from the conventional gasoline vehicles whose emissions are mainly released from vehicle tailpipes, emissions of battery electric vehicles (BEVs) are from the upstream processes of electricity generation and vehicle manufacturing, thus a comprehensive lifecycle analysis and comparison of BEVs with gasoline vehicles is required to quantify the emission mitigation benefit of vehicle electrification and determine the path to a carbon-neutral future. In the study, we compare the cradle-to-grave (C2G) lifecycle greenhouse gas emissions of gasoline and electric vehicles in China and analyze the greenhouse gas emission reduction of vehicle electrification in different provinces. Results show that under the current technologies, the national average C2G GHG emissions for battery electric vehicles (BEVs) of 100 miles (i.e., 160 km) and 300 miles (i.e., 480 km) all-electric range (AER) are 231 and 279 g CO2eq/km, respectively, 22% and 5% lower than those for gasoline internal combustion engine vehicles (ICEVs). Improving vehicle fuel efficiency by hybridizing gasoline ICEVs can effectively reduce C2G emissions to 212 g CO2eq/km. At the provincial level, C2G GHG emissions of BEVs vary according to the provincial electricity mix. In eight provinces, C2G GHG emissions of BEVs with 300 miles AER (BEV300s) are higher than those of gasoline ICEVs due to the GHG-intensive coal-based electricity mix. In the future scenario, with low carbon fuels (such as high-level bioethanol blending gasoline) and electricity decarbonization, the national average C2G emissions of hybrid electric vehicles (HEVs) and BEV300s can be reduced to 55 and 73 g CO2eq/km, respectively. Further decrease of C2G GHG emissions relies on reducing vehicle-cycle emissions from material processing and vehicle component manufacturing.

33 ADVANCED PROPULSION SYSTEMS↗
DOE OSTI2022

Prospects for Heavy Neutral SUSY HIGGS Scalars in the hMSSM and Natural SUSY at LHC Upgrades

We examine production and decay of heavy neutral SUSY Higgs bosons pp→H,A→ττ¯ within the hMSSM and compare against a perhaps more plausible natural supersymmetry scenario dubbed mh125(nat) which allows for a natural explanation for mweak≃mW,Z,h∼100 GeV while maintaining mh≃125 GeV. We evaluate signal against various Standard Model backgrounds from γ,Z→ττ¯, tt¯ and vector boson pair production VV. We combine the transverse mass method for back-to-back (BtB) taus along with the ditau mass peak mττ method for acollinear taus as our signal channels. This technique ultimately gives a boost to the signal significance over the standard technique of using just the BtB signal channel. We evaluate both the 95% CL exclusion and 5σ discovery reach in the mA vs. tanβ plane for present LHC with 139 fb−1, Run 3 with 300 fb−1 and high luminosity LHC (HL-LHC) with 3000 fb−1 of integrated luminosity. For tanβ=10, the exclusion limits range up to mA∼1, 1.1 and 1.4 TeV, respectively. These may be compared to the range of mA values gleaned from a statistical analysis of the string landscape wherein mA can range up to ∼8 TeV.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2023

Detecting Heavy Neutral SUSY Higgs Bosons Decaying to Sparticles at the High-Luminosity LHC

In supersymmetry (SUSY) models with low electroweak naturalness (natSUSY), which have been suggested to be the most likely version of SUSY to emerge from the string landscape, higgsinos are expected at the few hundred GeV scale, whilst electroweak gauginos inhabit the TeV scale. For TeV-scale heavy neutral SUSY Higgs bosons H and A, as currently required by LHC searches, the dominant decay modes of H,A are gaugino plus higgsino provided these decays are kinematically open. The light higgsinos decay to soft particles, so are largely invisible, whilst the gauginos decay to W, Z or h plus missing transverse energy (ET). Thus, we examine the viability of H,A→W+ET, Z+ET and h+ET signatures at the high luminosity LHC (HL-LHC) in light of large standard model (SM) backgrounds from (mainly) tt¯, VV and Vh production (where V=W,Z). We also examine whether these signal channels can be enhanced over backgrounds by requiring the presence of an additional soft lepton from the decays of the light higgsinos. We find significant regions in the vicinity of mA∼1–2 TeV of the mA vs. tanβ plane, which can be probed at the high luminosity LHC, using these dominant signatures by HL-LHC at 5σ and at the 95% confidence level (CL).

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2020

Plant Biosystems Design for a Carbon-Neutral Bioeconomy

Our society faces multiple daunting challenges including finding sustainable solutions towards climate change mitigation; efficient production of food, biofuels, and biomaterials; maximizing land-use efficiency; and enabling a sustainable bioeconomy. Plants can provide environmentally and economically sustainable solutions to these challenges due to their inherent capabilities for photosynthetic capture of atmospheric CO 2 , allocation of carbon to various organs and partitioning into various chemical forms, including contributions to total soil carbon. In order to enhance crop productivity and optimize chemistry simultaneously in the above- and belowground plant tissues, transformative biosystems design strategies are needed. Concerted research efforts will be required for accelerating the development of plant cultivars, genotypes, or varieties that are cooptimized in the contexts of biomass-derived fuels and/or materials aboveground and enhanced carbon sequestration belowground. Here, we briefly discuss significant knowledge gaps in our process understanding and the potential of synthetic biology in enabling advancements along the fundamental to applied research arc. Ultimately, a convergence of perspectives from academic, industrial, government, and consumer sectors will be needed to realize the potential merits of plant biosystems design for a carbon neutral bioeconomy.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

3D Exact Analytical Solutions of Two-fluid Plasma, Magnetohydrodynamics, and Neutral Fluid Equations for the Creation of Ordered Structures as well as Jet-like Flows

The 3D exact analytical solutions of ideal two-fluid plasma, single-fluid plasma, and neutral fluid equations have been found using physically justifiable assumptions. Surprisingly these solutions satisfy all nonlinearities in the systems. It is pointed out that these solutions explain the fundamental mechanism behind the creation of a vast variety of ordered structures in plasmas and fluids. In the limiting case of 2D dependence of fields, the theoretical model for plasma is applied to explain the formation of spicules in the solar chromosphere. It is pointed out that the main contribution of electron (ion) baroclinic vectors is to produce vorticity in the plasma, and that magnetic field generation is coupled with the flow of both electrons and ions.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2023

BICEP/Keck. XVI. Characterizing Dust Polarization through Correlations with Neutral Hydrogen

We characterize Galactic dust filaments by correlating BICEP/Keck and Planck data with polarization templates based on neutral hydrogen (H i) observations. Dust polarization is important for both our understanding of astrophysical processes in the interstellar medium (ISM) and the search for primordial gravitational waves in the cosmic microwave background (CMB). In the diffuse ISM, H i is strongly correlated with the dust and partly organized into filaments that are aligned with the local magnetic field. We analyze the deep BICEP/Keck data at 95, 150, and 220 GHz, over the low-column-density region of sky where BICEP/Keck has set the best limits on primordial gravitational waves. We separate the H i emission into distinct velocity components and detect dust polarization correlated with the local Galactic H i but not with the H i associated with Magellanic Stream i. We present a robust, multifrequency detection of polarized dust emission correlated with the filamentary H i morphology template down to 95 GHz. For assessing its utility for foreground cleaning, we report that the Hi morphology template correlates in B modes at a ~10%–65% level over the multipole range 20 < ℓ < 200 with the BICEP/Keck maps, which contain contributions from dust, CMB, and noise components. We measure the spectral index of the filamentary dust component spectral energy distribution to be β = 1.54 ± 0.13. We find no evidence for decorrelation in this region between the filaments and the rest of the dust field or from the inclusion of dust associated with the intermediate velocity H i. Finally, we explore the morphological parameter space in the H i-based filamentary model.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

Filamentary Dust Polarization and the Morphology of Neutral Hydrogen Structures

Filamentary structures in neutral hydrogen (H$\tiny{I}$) emission are well aligned with the interstellar magnetic field, so H$\tiny{I}$ emission morphology can be used to construct templates that strongly correlate with measurements of polarized thermal dust emission. We explore how the quantification of filament morphology affects this correlation. We introduce a new implementation of the Rolling Hough Transform (RHT) using spherical harmonic convolutions, which enables efficient quantification of filamentary structure on the sphere. We use this Spherical RHT algorithm along with a Hessian-based method to construct H$\tiny{I}$-based polarization templates. We discuss improvements to each algorithm relative to similar implementations in the literature and compare their outputs. By exploring the parameter space of filament morphologies with the Spherical RHT, we find that the most informative H$\tiny{I}$ structures for modeling the magnetic field structure are the thinnest resolved filaments. For this reason, we find a ~10% enhancement in the B-mode correlation with polarized dust emission with higher-resolution H$\tiny{I}$ observations. We demonstrate that certain interstellar morphologies can produce parity-violating signatures, i.e., nonzero TB and EB, even under the assumption that filaments are locally aligned with the magnetic field. Finally, we demonstrate that B modes from interstellar dust filaments are mostly affected by the topology of the filaments with respect to one another and their relative polarized intensities, whereas E modes are mostly sensitive to the shapes of individual filaments.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

The Effects of Turbulence on Heliosheath Ions and Implications for Energetic Neutral Atoms

The distribution of ions in the heliosheath—the region between the heliospheric termination shock and the heliopause—is important for understanding remote observations of energetic neutral atoms (ENAs). The ion distributions were estimated previously based on hybrid simulations of the heating and evolution of solar wind and interstellar pickup ions across the solar wind termination shock, but these estimates only provide the distributions near the shock. In this work, we use self-consistent hybrid kinetic simulations to investigate the effects of turbulence on ion distributions in the heliosheath. The simulations are compared against Voyager observations, constraining the feasible amplitude and compressibility of turbulence. We find that the heating due to turbulent dissipation can lead to a significant increase in the temperature of thermal solar wind ions. Both turbulent velocity fluctuations and the heating of solar wind ions increase the charge-exchange source for ENAs at low energies (around 100 eV), where current ENA models underpredict observations by more than an order of magnitude. However, the effects of turbulence are likely not strong enough to fully explain these discrepancies.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

Fourteen Years of Energetic Neutral Atom Observations from IBEX

The Interstellar Boundary Explorer (IBEX) has been observing the outer heliosphere and its interactions with the very local interstellar medium (VLISM) via measurements of energetic neutral atoms (ENAs) for over 14 yr. We discovered the IBEX Ribbon—a structure completely unanticipated by any prior theory or model—that almost certainly resides beyond the heliopause in the VLISM. We also characterized the other major source of heliospheric ENAs, the globally distributed flux (GDF), produced largely in the heliosheath between the termination shock and heliopause. In this study, we make three major new contributions. First, we validate, provide, and analyze the most recent 3 yr of IBEX-Hi (0.5–6 keV FWHM) data (2020–2022) for the first time. Second, we link these observations to the prior 11 yr of observations, exploring long-term variations. Finally, we provide the first IBEX team-validated Ribbon/GDF separation scheme and separated maps. Because of the uncertainty in separating different line-of-sight integrated sources, we provide not just best guess (median) maps, but also maps with upper and lower reasonable values of Ribbon and GDF fluxes, along with bounding fluxes that add the uncertainties to the upper and lower values. This allows theories and models to be compared with a range of possible values that the IBEX team believes are consistent with data. These observations, along with the reanalysis of the prior 11 yr of IBEX-Hi data, provide new insights and even further develop our detailed understanding of the heliosphere’s interaction with the local interstellar medium unlocked by IBEX.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

Review of searches for vector-like quarks, vector-like leptons, and heavy neutral leptons in proton-proton collisions at $\sqrt{s}$ = 13 TeV at the CMS experiment

The LHC has provided an unprecedented amount of proton-proton collision data, bringing forth exciting opportunities to address fundamental open questions in particle physics. These questions can potentially be answered by performing searches for very rare processes predicted by models that attempt to extend the standard model of particle physics. The data collected by the CMS experiment in 2015-2018 at a center-of-mass energy of 13 TeV can be used to test the standard model with high precision and potentially uncover evidence for new particles or interactions. An interesting possibility is the existence of new fermions with masses ranging from the MeV to the TeV scale. Such new particles appear in many possible extensions of the standard model and are well motivated theoretically. New fermions may explain the appearance of three generations of leptons and quarks, the mass hierarchy across the generations, and the nonzero neutrino masses. In this report, the results of searches targeting vector-like quarks, vector-like leptons, and heavy neutral leptons at the CMS experiment are summarized. The complementarity of current searches for each type of new fermion is discussed, and combinations of several searches for vector-like quarks are presented. The discovery potential for some of these searches at the High-Luminosity LHC is also discussed.

FOS: Physical sciences↗
DOE OSTI2025

First Search for Neutral Current Coherent Single-Photon Production in MicroBooNE

This article presents the first search for neutrino-induced neutral current coherent single-photon production (NC coherent 1$\gamma$). The search makes use of data from the MicroBooNE 85-tonne active volume liquid argon time projection chamber detector, situated in the Fermilab Booster Neutrino Beam (BNB), with an average neutrino energy of $\langle E_{\nu}\rangle \sim 0.8$ GeV. A targeted selection of candidate neutrino interactions with a single photon-like electromagnetic shower in the final state and no visible vertex activity was developed to search for the NC coherent 1$\gamma$ process, along with two auxiliary selections used to constrain the dominant background from NC$\pi^0$ production. With an integrated exposure of $6.87 \times 10^{20}$ protons on target delivered by the BNB, we set the world's first limit for this rare process, corresponding to an upper limit on the flux-averaged cross section of $\sigma<1.49 \times 10^{-41}\text{cm}^2$ at 90% C.L.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2020

An ultralong CDRH2 in HCV neutralizing antibody demonstrates structural plasticity of antibodies against E2 glycoprotein

A vaccine protective against diverse HCV variants is needed to control the HCV epidemic. Structures of E2 complexes with front layer-specific broadly neutralizing antibodies (bNAbs) isolated from HCV-infected individuals, revealed a disulfide bond-containing CDRH3 that adopts straight (individuals who clear infection) or bent (individuals with chronic infection) conformation. To investigate whether a straight versus bent disulfide bond-containing CDRH3 is specific to particular HCV-infected individuals, we solved a crystal structure of the HCV E2 ectodomain in complex with AR3X, a bNAb with an unusually long CDRH2 that was isolated from the chronically-infected individual from whom the bent CDRH3 bNAbs were derived. The structure revealed that AR3X utilizes both its ultralong CDRH2 and a disulfide motif-containing straight CDRH3 to recognize the E2 front layer. These results demonstrate that both the straight and bent CDRH3 classes of HCV bNAb can be elicited in a single individual, revealing a structural plasticity of VH1-69-derived bNAbs.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Deep electric field predictions by drift-reduced Braginskii theory with plasma-neutral interactions based upon experimental images of boundary turbulence

We present 2-dimensional turbulent electric field calculations via physics-informed deep learning consistent with (i) drift-reduced Braginskii theory under the framework of an axisymmetric fusion plasma with purely toroidal field and (ii) experimental estimates of the fluctuating electron density and temperature obtained from analysis of gas puff imaging of a discharge on the Alcator C-Mod tokamak. The inclusion of effects from the locally puffed atomic helium on particle and energy sources within the reduced plasma turbulence model are found to strengthen correlations between the electric field and electron pressure. The neutrals are also directly associated with an observed broadening in the distribution of turbulent field amplitudes and increased E×B shearing rates.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗