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NASA NTRS1997

Comparison of Snow Mass Estimates from a Prototype Passive Microwave Snow Algorithm, a Revised Algorithm and a Snow Depth Climatology

While it is recognized that no single snow algorithm is capable of producing accurate global estimates of snow depth, for research purposes it is useful to test an algorithm's performance in different climatic areas in order to see how it responds to a variety of snow conditions. This study is one of the first to develop separate passive microwave snow algorithms for North America and Eurasia by including parameters that consider the effects of variations in forest cover and crystal size on microwave brightness temperature. A new algorithm (GSFC 1996) is compared to a prototype algorithm (Chang et al., 1987) and to a snow depth climatology (SDC), which for this study is considered to be a standard reference or baseline. It is shown that the GSFC 1996 algorithm compares much more favorably to the SDC than does the Chang et al. (1987) algorithm. For example, in North America in February there is a 15% difference between the GSFC 198-96 Algorithm and the SDC, but with the Chang et al. (1987) algorithm the difference is greater than 50%. In Eurasia, also in February, there is only a 1.3% difference between the GSFC 1996 algorithm and the SDC, whereas with the Chang et al. (1987) algorithm the difference is about 20%. As expected, differences tend to be less when the snow cover extent is greater, particularly for Eurasia. The GSFC 1996 algorithm performs better in North America in each month than dose the Chang et al. (1987) algorithm. This is also the case in Eurasia, except in April and May when the Chang et al.(1987) algorithms is in closer accord to the SDC than is GSFC 1996 algorithm.

Foster, J. L.↗
NASA NTRS2000

Advanced Methods for Acoustic and Thrust Benefits for Aircraft Engine Nozzles

The Fluid Mechanics and Acoustics Laboratory (FM&AL) was established At Hampton University in June of 1996. In addition, the FM&AL jointly conducted research with the Central AeroHydrodynamics Institute (TsAGI, Moscow) in Russia under a 2.5 year Civilian Research and Development Foundation (CRDF). The goals of the FM&AL programs are two fold: 1) to improve the working efficiency of the FM&AL team in generating new innovative ideas and in conducting research in the field of fluid dynamics and acoustics, basically for improvement of supersonic and subsonic aircraft engines, and 2) to attract promising minority students to this research and training and, in cooperation with other HU departments, to teach them basic knowledge in Aerodynamics, Gas Dynamics, and Theoretical and Experimental Methods in Aeroacoustics and Computational Fluid Dynamics (CFD). The research at the FM&AL supports reduction schemes associated with the emission of engine pollutants for commercial aircraft and concepts for reduction of observables for military aircraft. These research endeavors relate to the goals of the NASA Strategic Enterprise in Aeronautics concerning the development of environmentally acceptable aircraft. It is in this precise area, where the US aircraft industry, academia, and Government are in great need of trained professionals and which is a high priority goal of the Minority University Research and Education (MUREP) Program, that the HU FM&AL can make its most important contribution. This project already benefits NASA and HU because: First, the innovation, testing, and further development of new techniques for advanced propulsion systems are necessary for the successful attainment of the NASA Long Term Goals in Aeronautics and Space Transportation Technology (ASTT) including Global Civil Aviation, Revolutionary Technology Leaps, Access to Space, R&D Services, and the economic competitiveness of the US Aircraft Industry in the 2 1 st century. Secondly, the joint theoretical and experimental research and training by the GRC-HU Teams aids: using advanced methods and experience in Aerospace Engineering for domestic industries and training of HU students for interesting innovative work in the numerical simulation field as well as engineering and experimental research. HU students use and modify existing numerical codes for the solution of actual applied problems of the NASA Langley Research Center (LaRC)

Morgan, Morris H., III↗
NASA NTRS2000

Mixing, Noise and Thrust Benefits Using Corrugated Designs

These projects are directed toward the analysis of several concepts for nozzle and inlet performance improvement and noise reduction from jet exhausts. Currently. The FM&AL also initiates new joint research between the HU/FM&AL, the Hyper-X Program Team at the LaRC, and the Central Institute of Aviation Motors (CIAM), Moscow, Russia in the field of optimization of fuel injection and mixing in air-breathing propulsion systems. The main results of theoretical, numerical simulation and experimental tests obtained in the previous research are in the papers and patents. The goals of the 14U/FM&AL programs are twofold: 1) to improve the working efficiency of the HU/FM&AL team in generating new innovative ideas and in conducting research in the field of fluid dynamics and acoustics, basically for improvement of supersonic and subsonic aircraft engines, and 2) to attract promising minority students to this research and training and, in cooperation with other HU departments, to teach them basic knowledge in Aerodynamics, Gas Dynamics, and Theoretical and Experimental Methods in Aeroacoustics and Computational Fluid Dynamics (CFD). The research at the HU/FM&AL supports reduction schemes associated with the emission of en 'ne pollutants for commercial aircraft and concepts for reduction of 91 observables for military aircraft. These research endeavors relate to the goals of the NASA Strategic Enterprise in Aeronautics concerning the development of environmentally acceptable aircraft. It is in this precise area, where the US aircraft industry, academia, and Government are in great need of trained professionals and which is a high priority goal of the Minority University Research and Education (MLTREP) Program, that the HU/FM&AL can make its most important contribution.

Morgan, Morris H., III↗
NASA NTRS2000

Meteorological Characterization of Global Dust Sources

The global distribution of absorbing aerosols index (AI) derived from Nimbus 7 Total Ozone Mapping Spectrometer (TOMS) measurements has been used to identify the major sources of atmospheric dust particles [Prospero, et al., 2000]. In this presentation, we will show that the TOMS aerosol index can also be used to the meteorological characterization of the source areas. We found a huge "dust belt" with a strong correlation between the TOMS index and the sea-level pressure wrapping all the major dust sources. To handle the difficulty of using quantitatively the TOMS Al and its strong dependency on the aerosol layer altitude, the Al has been correlated to the planetary boundary layer depth (PBL) and the surface wind speed. The strongest correlation is obtained with the product of these two variables and at the precise location of the dust sources identified by Prospero et al. The relation between the TOMS Al and the product of the PBL height and the surface wind speed has been used to study the interannual variability of the North-Africa dust sources in relation with the North Atlantic Oscillation (NAO). Finally, we will show that even on a short time base the variability of the TOMS Al can be related to the surface wind speed and PBL height.

Ginoux, Paul↗
NASA NTRS2000

Dynamic Modeling of Off-Nominal Operation in Advanced Life Support Systems

System failures, off-nominal operation, or unexpected interruptions in processing capability can cause unanticipated instabilities in Advanced Life Support (ALS) systems, even long after they are repaired. Much current modeling assumes ALS systems are static and linear, but ALS systems are actually dynamic and nonlinear, especially when failures and off nominal operation are considered. Modeling and simulation provide a way to study the stability and time behavior of nonlinear dynamic ALS systems under changed system configurations or operational scenarios. The dynamic behavior of a nonlinear system can be fully explored only by computer simulation over the full range of inputs and initial conditions. Previous simulations of BIO-Plex in SIMULINK, a toolbox of Matlab, were extended to model the off-nominal operation and long-term dynamics of partially closed physical/chemical and bioregenerative life support systems. System nonlinearity has many interesting potential consequences. Different equilibrium points may be reached for different initial conditions. The system stability can depend on the exact system inputs and initial conditions. The system may oscillate or even in rare cases behave chaotically. Temporary internal hardware failures or external perturbations in ALS systems can lead to dynamic instability and total ALS system failure. Appropriate control techniques can restore reliable operation and minimize the effects of dynamic instabilities due to anomalies or perturbations in a life support system.

Jones, Harry↗
NASA NTRS2000

Consensus Assignments for Water Vapor Lines Not Assigned on the HITRAN Database: 13,200 to 16,500/cm

There are nearly 800 water Vapor-lines in the 13,200-16,500/cm region that do not have rovibrational assignments in the HITRAN database. The positions and intensities in the database were determined by Mandin et al., but assignments could not be determined at that time. Polyansky, et al. have now assigned over 600 of the unassigned lines in the 11,200-16,500/cm region. Schwenke has also given rovibrational assignments to many of these unassigned lines throughout the visible and near-infrared. Both articles changed the assignments of some HITRAN lines. Carleer et al. extend assignments to some weaker lines measured by them on new spectra with excellent signal/noise. However, some lines measured by Mandin et al. were omitted by Carleer, et al. because of blends due to lower spectral resolution. The rovibrational assignments of Polyansky et al. completely agree with those in Schwenke's article for only about 200 lines. However, Schwenke's ab initio line list is available on his internet site (http://ccf.arc.nasa.gov/-dschwenke). A detailed comparison of the Polyansky et al.line list, the Carleer et al.line list, and Schwenke's ab initio line list shows a larger number of agreements. In many cases the disagreement is only about the vibrational and/or rotational upper level, while there is agreement on the lower state assignment and energy level, "E", which is of primary importance for atmospheric applications. We will present a line list of "consensus" assignments in the 13,200-16,500/cm region for consideration of inclusion on the HITRAN and GEISA databases. This will substantially reduce the number of unassigned lines on the databases in this spectral region.

Giver, Lawerence P.↗
NASA NTRS2001

Advanced Methods for Aircraft Engine Thrust and Noise Benefits: Nozzle-Inlet Flow Analysis

The Fluid Mechanics and Acoustics Laboratory at Hampton University (HU/FM&AL) jointly with the NASA Glenn Research Center has conducted four connected subprojects under the reporting project. Basically, the HU/FM&AL Team has been involved in joint research with the purpose of theoretical explanation of experimental facts and creation of accurate numerical simulation techniques and prediction theory for solution of current problems in propulsion systems of interest to the NAVY and NASA agencies. This work is also supported by joint research between the NASA GRC and the Institute of Mechanics at Moscow State University (IM/MSU) in Russia under a CRDF grant. The research is focused on a wide regime of problems in the propulsion field as well as in experimental testing and theoretical and numerical simulation analyses for advanced aircraft and rocket engines. The FM&AL Team uses analytical methods, numerical simulations and possible experimental tests at the Hampton University campus. The fundamental idea uniting these subprojects is to use nontraditional 3D corrugated and composite nozzle and inlet designs and additional methods for exhaust jet noise reduction without essential thrust loss and even with thrust augmentation. These subprojects are: (1) Aeroperformance and acoustics of Bluebell-shaped and Telescope-shaped designs; (2) An analysis of sharp-edged nozzle exit designs for effective fuel injection into the flow stream in air-breathing engines: triangular-round, diamond-round and other nozzles; (3) Measurement technique improvement for the HU Low Speed Wind Tunnel; a new course in the field of aerodynamics, teaching and training of HU students; experimental tests of Mobius-shaped screws: research and training; (4) Supersonic inlet shape optimization. The main outcomes during this reporting period are: (l) Publications: The AIAA Paper #00-3170 was presented at the 36th AIAA/ASME/SAE/ASEE Joint Propulsion Conference, 17-19 June, 2000, Huntsville, AL. The AIAA Paper #01-1893 has been accepted for the AIAA/NAL-NASDA-ISAS 10th International Space Planes and Hypersonic Systems and Technologies Conference, 24-27 April 2001, Kyoto, Japan. The AIAA Paper #01 -3204 has been accepted for presentation at the 37th AIAA/ASME/SAE/ASEE Joint Propulsion Conference, being held on 08-11 July, in Salt Lake City, UT; (2) A U.S. patent #6,082,635 was granted on July 4, 2000; (3) Grants and proposals: The H U/ FM&AL was awarded the NASA grant NAG-3-2495 in October 2000 and the laboratory is a primary U.S. research team in a joint project under the CRDF award granted to the NASA GRC and IM/MSU (Russia) in July 2000; (4) Theory and numerical simulations: Analytical theory, numerical simulation, comparison of theoretical with experimental results, and modification of theoretical approaches, models, grids, etc., have been conducted for several complicated 2D and 3D nozzle and inlet designs using NASA, ICASE, and IM/MSU codes based on full Euler and Navier-Stokes solvers: CFL3D, FLUENT, and GODUNOV, and others; (5) Experimental Tests: (a) A new course: "Advanced Aerodynamics and Aircraft Performance" presented in spring semester, 2001; training and experimental test research using the HU LSWT. (b) Small-scale M6bius-shaped screws were tested in different conditions and their application has shown essential benefits by comparison with traditional designs; (6) Installation in the FM&AL computer system: second software TECPLOT 8.0 for the UNIX SGI workstation and free TECPLOT 7.5 for the PC Dell computer, and 2D and 3D GRIDGEN (version 9) for the UNIX SGI as well as installation of two free NASA codes, 3D MAG and VULCAN; (7) Student Research Activity: Involvement of two undergraduate students as research assistants in the current research project.

Gilinsky, Mikhail↗
NASA NTRS2001

Numerical Simulation of One- and Two-Phase Flows in Propulsion Systems

In this report, we present some results of problems investigated during joint research between the Hampton University Fluid Mechanics and Acoustics Laboratory (HU/FM&AL), NASA GRC, and the LaRC Hyper-X Program. This work is supported by joint research between the NASA GRC and the Institute of Mechanics at Moscow State University (IM/MSU) in Russia under a CRDF grant. The main areas of current scientific interest of the HU/FM&AL include an investigation of the proposed and patented advanced methods for aircraft engine thrust and noise benefits. These methods are based on nontraditional 3D corrugated and composite nozzle, inlet, propeller and screw designs such as a Bluebell and Telescope nozzle, Mobius-shaped screw, etc. This is the main subject of our other projects, of which one is presented at the current conference. Here we analyze additional methods for exhaust jet noise reduction without essential thrust loss and even with thrust augmentation. Such additional approaches are: (1) to add some solid, fluid, or gas mass at discrete locations to the main supersonic gas stream to minimize the negative influence of strong shock waves formed in propulsion systems. This mass addition may be accompanied by heat addition to the main stream as a result of the fuel combustion or by cooling of this stream as a result of the liquid mass evaporation and boiling; (2) Use of porous or permeable nozzles and additional shells at the nozzle exit for preliminary cooling of the hot jet exhaust and pressure compensation for off-design conditions (so-called continuous ejector with small mass flow rate); and (3) to propose and analyze new effective methods of fuel injection into the flow stream in air-breathing engines. The research is focused on a wide regime of problems in the propulsion field as well as in experimental testing and theoretical and numerical simulation analyses for advanced aircraft and rocket engines. The FM&AL Team uses analytical methods, numerical simulations, and experimental tests at the Hampton University campus, NASA, and IM/MSU. The main outcomes during this reporting period are: (1) Publications: The AIAA Paper #01-1893 has been accepted for the AIAA/NAL-NASDA-ISAS 10th International Space Planes and Hypersonic Systems and Technologies Conference, 24-27 April 2001, Kyoto, Japan. The AIAA Paper #01-3204 has been accepted for presentation at the 37th AIAA/ASME/SAE/ASEE Joint Propulsion Conference being held on 08-11 July, in Salt Lake City, UT; (2) Grants and proposals: The HU/FM&AL was awarded the NASA grant NAG3-2495 in October 2000 and the CRDF award was granted to the NASA GRC-HU FM&AL and IM/MSU (Russia) in July 2000. A solicited proposal was submitted for the NASA NRA-01GRC-02 competition and two unsolicited proposals to NASA are in preparation; (3) Theory and numerical simulations: Analytical theory, numerical simulation, comparison of theoretical with experimental results, and modification of theoretical approaches, models, grids, etc. Such investigations have been conducted for three main problems: (a) Combustion efficiency optimization in the half-duct combustor system; (b) Drag reduction effects for blunt bodies with solid needles; and (c) Solid particle injection from the butt-end against a supersonic flow. The NASA CFL3D, HU/FM&AL, and IM/MSU GODUNOV codes were used and modified for solution of these problems. The codes are based on full Euler and Navier-Stokes solvers with and without nonequilibrium oxygen-nitrogen and air-hydrogen chemical reactions in laminar and turbulent gas flow regimes; (4) Experimental tests: In the IM/MSU supersonic wind tunnel, experimental tests were conducted with different number, location, and geometric parameters of solid needles mounted at the front of the butt-end in supersonic flow. Optimal parameters were determined that provide minimal butt-end drag in the stationary flow regime. Experimental and numerical simulation results are in good agreement; (5) Student Research Activity: Involvement of one graduate and two undergraduate students as research assistants in the current project.

Gilinsky, Mikhail↗
NASA NTRS1999

Advanced Life Support System Value Metric

The NASA Advanced Life Support (ALS) Program is required to provide a performance metric to measure its progress in system development. Extensive discussions within the ALS program have led to the following approach. The Equivalent System Mass (ESM) metric has been traditionally used and provides a good summary of the weight, size, and power cost factors of space life support equipment. But ESM assumes that all the systems being traded off exactly meet a fixed performance requirement, so that the value and benefit (readiness, performance, safety, etc.) of all the different systems designs are considered to be exactly equal. This is too simplistic. Actual system design concepts are selected using many cost and benefit factors and the system specification is defined after many trade-offs. The ALS program needs a multi-parameter metric including both the ESM and a System Value Metric (SVM). The SVM would include safety, maintainability, reliability, performance, use of cross cutting technology, and commercialization potential. Another major factor in system selection is technology readiness level (TRL), a familiar metric in ALS. The overall ALS system metric that is suggested is a benefit/cost ratio, SVM/[ESM + function (TRL)], with appropriate weighting and scaling. The total value is given by SVM. Cost is represented by higher ESM and lower TRL. The paper provides a detailed description and example application of a suggested System Value Metric and an overall ALS system metric.

Jones, Harry W.↗
NASA NTRS2002

The 1996-2002 Plunge in the North-Atlantic Oscillation Index Produces Cold Spring Temperatures in Central Europe

Surface-air temperatures in winter and spring in central Europe rose over the second half of the 20th century, reported for different data-spans, and by different approaches (Ross et al., 1996; Angell, 1999; Hansen et al., 1999; Demaree et al., 2002). Analysis with a finer temporal resolution shows that late-winter and early-spring (February and March) trends are much stronger than the 3-month season averages (Otterman et al., 2002a). Responding to this need for finer than 3- month resolution, observations at meteorological stations in central Europe are analyzed here for the years 1951-2002, computing six-pentad (5-day period) averages (effectively monthly averages for January, February, and March). The daily minimum surface-air temperature, T(sub min), and the daily maximum temperature, T(sub max), rose steeply in Berlin and Poznan' in the years 1951-1995. Based on sensitivity studies, the bulk of this sharp warming is due to stronger southwesterlies over the North Atlantic, with which the temperatures in Europe are strongly correlated (Otterman et al., 1999; 2002a). However, for the most recent seven years, a pronounced downturn of the warming is observed, which we attribute to the concurrent, 1996-2002, sharp downturn of the ocean-surface southwesterlies over the North Atlantic (Otterman et al., 2002b). Such changes in the ocean winds and variations in the storm tracks are associated with changes in the North Atlantic Oscillation, NAO (Rogers, 1997). The NAO index, the difference in the surface pressure between Iceland and Azores, constitutes a measure of the zonal winds over the eastern North Atlantic, and thus is a critically important factor influencing the flow of maritime air into Europe (but the temperature of the advected airmasses depends on the meridional component, as we discuss). The recent (1996-2002) downturn in this index resulted in much colder spring temperatures in Europe, with adverse significance for the growing season.

Atlas, Robert↗
NASA NTRS2004

Selection of Optical Cavity Surface Coatings for 1micron Laser Based Missions

The particulate surface cleanliness level on several coatings for aluminum and beryllium substrates were examined for use in the optical cavities of high pulse energy Nd:YAG Q-switched, diode-pumped lasers for space flight applications. Because of the high intensity of the lasers, any contaminants in the laser beam path could damage optical coatings and limit the instrument mission objectives at the operating wavelength of 1 micron (micrometer). Our goal was to achieve an EST-STD-CC1246D Level 100 particulate distribution or better to ensure particulate redistribution during launch would not adversely affect the performance objectives. Tapelifts were performed to quantify the amount of particles using in-house developed procedures. The primary candidate coatings included chromate conversion coating aluminum (Al), uncoated Al electroless Nickel (Ni) on Al, Ni-gold (Au) on Al, anodized Al, and gold (Au)/Ni on Beryllium (Be). The results indicate that there were advantages in Ni and Au coating applications for the two major substrates, Al and Be, when considering applications that need to meet launch environments.

Hedgeland, Randy J.↗
NASA NTRS2002

Retinoid quantification by HPLC/MS(n)

Retinoic acid (RA) mediates most of the biological effects of vitamin A that are essential for vertebrate survival. It acts through binding to receptors that belong to the nuclear receptor transcription factor superfamily (Mangelsdorf et al. 1994). It is also a highly potent vertebrate teratogen. To determine the function and effects of endogenous and exogenous RA, it is important to have a highly specific, sensitive, accurate, and precise analytical procedure. Current analyses of RA and other retinoids are labor intensive, of poor sensitivity, have limited specificity, or require compatibility with RA reporter cell lines (Chen et al. 1995. BIOCHEM: Pharmacol. 50: 1257-1264; Creech Kraft et al. 1994. BIOCHEM: J. 301: 111-119; Lanvers et al. 1996. J. Chromatogr. B Biomed. Appl. 685: 233-240; Maden et al. 1998. DEVELOPMENT: 125: 4133-4144; Wagner et al. 1992. DEVELOPMENT: 116: 55-66). This paper describes an HPLC/mass spectrometry/mass spectrometry product ion scan (HPLC/MS(n)) procedure for the analysis of retinoids that employs atmospheric pressure chemical ionization MS. The retinoids are separated by normal-phase column chromatography with a linear hexane-isopropanol-dioxane gradient. Each retinoid is detected by a unique series of MS(n) functions set at optimal collision-induced dissociation energy (30% to 32%) for all MS(n) steps. The scan events are divided into three segments, based on HPLC elution order, to maximize the mass spectrometer duty cycle. The all-trans, 9-cis, and 13-cis RA isomers are separated, if desired, by an isocratic hexane-dioxane-isopropanol mobile phase. This paper describes an HPLC/MS(n) procedure possessing high sensitivity and specificity for retinoids.

NASA Discipline Developmental Biology↗
NASA NTRS1999

Neutron yields from 155 MeV/nucleon carbon and helium stopping in aluminum

Neutron fluences have been measured from 155 MeV/nucleon 4He and 12C ions stopping in an Al target at laboratory angles between 10 and 160 deg. The resultant spectra were integrated over angle and energy above 10 MeV to produce total neutron yields. Comparison of the two systems shows that approximately two times as many neutrons are produced from 155 MeV/nucleon 4He stopping in Al and 155 MeV/nucleon 12C stopping in Al. Using an energy-dependent geometric cross-section formula to calculate the expected number of primary nuclear interactions shows that the 12C + Al system has, within uncertainties, the same number of neutrons per interaction (0.99 +/- 0.03) as does the 4He + Al system (1.02 +/- 0.04), despite the fact that 12C has three times as many neutrons as does 4He. Energy and angular distributions for both systems are also reported. No major differences can be seen between the two systems in those distributions, except for the overall magnitude. Where possible, the 4He + Al spectra are compared with previously measured spectra from 160 and 177.5 MeV/nucleon 4He interactions in a variety of stopping targets. The reported spectra are consistent with previously measured spectra. The data were acquired to provide data applicable to problems dealing with the determination of the radiation risk to humans engaged in long-term missions in space; however, the data are also of interest for issues related to the determination of the radiation environment in high-altitude flight, with shielding at high-energy heavy-ion accelerators and with doses delivered outside tumor sites treated with high-energy hadronic beams.

NASA Discipline Radiation Health↗
NASA NTRS2000

Dynamic markers of altered gait rhythm in amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a disorder marked by loss of motoneurons. We hypothesized that subjects with ALS would have an altered gait rhythm, with an increase in both the magnitude of the stride-to-stride fluctuations and perturbations in the fluctuation dynamics. To test for this locomotor instability, we quantitatively compared the gait rhythm of subjects with ALS with that of normal controls and with that of subjects with Parkinson's disease (PD) and Huntington's disease (HD), pathologies of the basal ganglia. Subjects walked for 5 min at their usual pace wearing an ankle-worn recorder that enabled determination of the duration of each stride and of stride-to-stride fluctuations. We found that the gait of patients with ALS is less steady and more temporally disorganized compared with that of healthy controls. In addition, advanced ALS, HD, and PD were associated with certain common, as well as apparently distinct, features of altered stride dynamics. Thus stride-to-stride control of gait rhythm is apparently compromised with ALS. Moreover, a matrix of markers based on gait dynamics may be useful in characterizing certain pathologies of motor control and, possibly, in quantitatively monitoring disease progression and evaluating therapeutic interventions.

NASA Program Biomedical Research and Countermeasur↗
NASA NTRS2000

Resistance of extraocular motoneuron terminals to effects of amyotrophic lateral sclerosis sera

In sporadic ALS (s-ALS), axon terminals contain increased intracellular calcium. Passively transferred sera from patients with s-ALS increase intracellular calcium in spinal motoneuron terminals in vivo and enhance spontaneous transmitter release, a calcium-dependent process. In this study, passive transfer of s-ALS sera increased spontaneous release from spinal but not extraocular motoneuron terminals, suggesting that the resistance to physiologic abnormalities induced by s-ALS sera in mice parallels the resistance of extraocular motoneurons to dysfunction and degeneration in ALS.

NASA Program Biomedical Research and Countermeasur↗
NASA NTRS1996

Hyaluronic acid is increased in the skin and urine in patients with amyotrophic lateral sclerosis

We performed morphological studies of skin and measured glycosaminoglycans in the urine from patients with sporadic amyotrophic lateral sclerosis (ALS) and control subjects. The wide spaces separating collagen bundles reacted strongly with alcian blue stain in ALS patients and stained more markedly as ALS progressed. Staining with alcian blue was virtually eliminated by Streptomyces hyaluronidase. The urinary excretion of hyaluronic acid (HA) (mg/day) was significantly increased (P < 0.01) in ALS patients compared with that of control subjects, and there was a significant positive correlation between the excreted amount of HA and the duration of illness in advanced ALS patients with a duration of more than 2 years from clinical onset (r = 0.72, P < 0.02). We suggest that sporadic ALS includes a metabolic disorder of HA in which an accumulation of HA in the skin is linked to an increased urinary excretion of HA.

NASA Discipline Musculoskeletal↗
NASA NTRS1992

Glutamate and aspartate are decreased in the skin in amyotrophic lateral sclerosis

We measured the levels of amino acids in biopsied skin from eight patients with amyotrophic lateral sclerosis (ALS) and seven controls. The most conspicuous changes in ALS patients were as follows. First, the contents of the acidic amino acids glutamate and aspartate were significantly decreased in ALS, and were negatively and significantly associated with the duration of illness. Second, the levels of the collagen-associated amino acids hydroxyproline, proline, glycine, alanine, and hydroxylysine were significantly decreased in ALS, and correlated inversely with the duration of illness. These results suggest that there are abnormalities of acidic amino acids and collagen-associated amino acids in the skin of patients with ALS. These changes may underlie the pathogenesis of ALS.

NASA Discipline Musculoskeletal↗
NASA NTRS1992

Amyotrophic lateral sclerosis: increased solubility of skin collagen

We studied the solubility of skin collagen from six patients with amyotrophic lateral sclerosis (ALS) and six controls. The amount of collagen extracted with neutral salt solution was significantly greater in patients with ALS than in controls. In addition, there was a statistically significant increase in the proportion of collagen extracted from ALS patients with increased duration of illness. The collagen solubilized by pepsin and cyanogen bromide treatments was significantly higher in ALS patients than in controls, and its proportion was positively and significantly associated with duration of illness in ALS patients. These results indicate that the metabolism of skin collagen may be affected in the disease process of ALS, causing an increase in immature soluble collagen in the tissue, which is the opposite to that which occurs in the normal aging process.

NASA Discipline Musculoskeletal↗