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At least 955 records · Page 53

Cyanobacteria and Chloroflexota cooperate to structure light-responsive biofilms

Microbial mats are stratified communities often dominated by unicellular and filamentous phototrophs within an exopolymer matrix. It is challenging to quantify the dynamic responses of community members in situ as they experience steep gradients and rapid fluctuations of light. To address this, we developed a binary consortium using two representative isolates from hot spring mats: the unicellular oxygenic phototrophic cyanobacterium Synechococcus OS-B′ (Syn OS-B′) and the filamentous anoxygenic phototroph Chloroflexus MS-CIW-1 (Chfl MS-1). We quantified the motility of individual cells and entire colonies and demonstrated that Chfl MS-1 formed bundles of filaments that moved in all directions with no directional bias to light. Syn OS-B′ was slightly less motile but exhibited positive phototaxis. This binary consortium displayed cooperative behavior by moving further than either species alone and formed ordered arrays where both species aligned with the light source. No cooperative motility was observed when a nonmotile pilB mutant of Syn OS-B′ was used instead of Syn OS-B′. The binary consortium also produced more adherent biofilm than individual species, consistent with the close interspecies association revealed by electron microscopy. We propose that cyanobacteria and Chloroflexota cooperate in forming natural microbial mats by colonizing new niches and building robust biofilms.

Functional and Systems Biology↗

Statistical energy analysis of complex structures, phase 2

A method for estimating the structural vibration properties of complex systems in high frequency environments was investigated. The structure analyzed was the Materials Experiment Assembly, (MEA), which is a portion of the OST-2A payload for the space transportation system. Statistical energy analysis (SEA) techniques were used to model the structure and predict the structural element response to acoustic excitation. A comparison of the intial response predictions and measured acoustic test data is presented. The conclusions indicate that: the SEA predicted the response of primary structure to acoustic excitation over a wide range of frequencies; and the contribution of mechanically induced random vibration to the total MEA is not significant.

Trudell, R. W.↗

Thermally induced response of flexible structures - A method for analysis

The phenomenon of a thermally induced spacecraft instability became apparent during the flight of the OGO-IV spacecraft in the late 1960s. The paper presents a generalized approach to the problem of obtaining a computationally practical simulation model for a thermally driven structure. The formulation is such that it can be utilized in a stand-along manner or be meshed into a general multibody simulation model such as that reported by Bodley et al. (1978). As presented, the formulation is restricted to the dynamic range for which linearized radiation is a valid assumption.

Frisch, H. P.↗

Vortex pairing in a circular jet under controlled excitation. I - General jet response. II - Coherent structure dynamics

Hot-wire and flow visualization studies were performed in three air jets subjected to pure-tone excitation. The instability, vortex roll-up, and transition to the controlled excitation were investigated. The conditions most favorable to vortex parting were determined as a function of the excitation Strouhal number, the Reynolds number, and the initial shear-layer state; it was shown that the rolled-up vortex rings undergo pairing under 'the shear layer mode', and the 'jet-column mode' when the Strouhal numbers based on the initial shear-layer momentum thickness are 0.012 and 0.85, respectively. Coherent ring-like vortical structures could be educed to the end of the potential core; however, the paired vortex becomes weaker with increasing downstream distances. The transverse transport of 'u' momentum by the coherent structures was much larger during the pairing process than in regions where a single vortex is studied.

Zaman, K. B. M. Q.↗

Composite mechanics for engine structures

Recent research activities and accomplishments at Lewis Research Center on composite mechanics for engine structures are summarized. The activities focused mainly on developing procedures for the computational simulation of composite intrinsic and structural behavior. The computational simulation encompasses all aspects of composite mechanics, advanced three-dimensional finite-element methods, damage tolerance, composite structural and dynamic response, and structural tailoring and optimization.

Chamis, Christos C.↗

Composite mechanics for engine structures

Recent research activities and accomplishments at Lewis Research Center on composite mechanics for engine structures are summarized. The activities focused mainly on developing procedures for the computational simulation of composite intrinsic and structural behavior. The computational simulation encompasses all aspects of composite mechanics, advanced three-dimensional finite-element methods, damage tolerance, composite structural and dynamic response, and structural tailoring and optimization.

Chamis, Christos C.↗

The effects of interply damping layers on the dynamic response of composite structures

Integrated damping mechanics for composite laminates with constrained interlaminar layers of polymer damping materials are developed. Discrete layer damping mechanics are presented for composite materials with damping layers, in connection with a semi-analytical method for predicting the modal damping in simply-supported specialty composite plates. Several application cases are used to demonstrate the advantages of the method. Damping predictions for graphite-epoxy composite plates of various laminations demonstrate the potential for higher damping than geometrically equivalent aluminum plates. The effects of aspect ratio, damping layer thickness, and fiber volume ratio on static and dynamic characteristics of the composite plate are also investigated.

Saravanos, D. A.↗

The effects of interply damping layers on the dynamic response of composite structures

Integrated damping mechanics for composite laminates with interlaminar constrained layers of damping polymer materials are developed. Discrete layer damping mechanics for composite laminates with damping layers, in connection with a semi-analytical method for predicting the modal damping in simply-supported specialty composite plates are presented. Application cases demonstrate the advantages of the method. Damping predictions for graphite/epoxy composite plates of various laminations demonstrate the potential for higher damping than geometrically equivalent aluminum plates. The effects of aspect ratio, damping layer thickness, and fiber volume ratio on static and dynamic characteristics of the composite plate are also investigated.

Saravanos, D. A.↗

Aeroelastic stability and response of rotating structures

A summary of the work performed during the progress period is presented. Analysis methods for predicting loads and instabilities of wind turbines were developed. Three new areas of research to aid the Advanced Turboprop Project (ATP) were initiated and developed. These three areas of research are aeroelastic analysis methods for cascades including blade and disk flexibility; stall flutter analysis; and computational aeroelasticity.

Keith, Theo G., Jr.↗

Natural Frequency Testing and Model Correlation of Rocket Engine Structures in Liquid Hydrogen - Phase I, Cantilever Beam

Many structures in the launch vehicle industry operate in liquid hydrogen (LH2), from the hydrogen fuel tanks through the ducts and valves and into the pump sides of the turbopumps. Calculating the structural dynamic response of these structures is critical for successful qualification of this hardware, but accurate knowledge of the natural frequencies is based entirely on numerical or analytical predictions of frequency reduction due to the added-fluid-mass effect because testing in LH2 has always been considered too difficult and dangerous. This fluid effect is predicted to be approximately 4-5% using analytical formulations for simple cantilever beams. As part of a comprehensive test/analysis program to more accurately assess pump inducers operating in LH2, a series of frequency tests in LH2 were performed at NASA/Marshall Space Flight Center's unique cryogenic test facility. These frequency tests are coupled with modal tests in air and water to provide critical information not only on the mass effect of LH2, but also the cryogenic temperature effect on Young's Modulus for which the data is not extensive. The authors are unaware of any other reported natural frequency testing in this media. In addition to the inducer, a simple cantilever beam was also tested in the tank to provide a more easily modeled geometry as well as one that has an analytical solution for the mass effect. This data will prove critical for accurate structural dynamic analysis of these structures, which operate in a highly-dynamic environment.

Brown, Andrew M.↗