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At least 955 records · Page 53

Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in Saccharomyces cerevisiae

Glycolysis is central to energy metabolism in most organisms and is highly regulated to enable optimal growth. In the yeast Saccharomyces cerevisiae, feedback mechanisms that control flux through glycolysis span transcriptional control to metabolite levels in the cell. Using a cellobiose consumption pathway, we decoupled glucose sensing from carbon utilization, revealing new modular layers of control that induce ATP consumption to drive rapid carbon fermentation. Alterations of the beta subunit of phosphofructokinase-1 (PFK2), H + -plasma membrane ATPase (PMA1), and glucose sensors (SNF3 and RGT2) revealed the importance of coupling extracellular glucose sensing to manage ATP levels in the cell. Controlling the upper bound of cellular ATP levels may be a general mechanism used to regulate energy levels in cells, via a regulatory network that can be uncoupled from ATP concentrations under perceived starvation conditions.

59 BASIC BIOLOGICAL SCIENCES↗

Spectroscopic detection of biological NO with a quantum cascade laser

Two configurations of a continuous wave quantum cascade distributed feedback laser-based gas sensor for the detection of NO at a parts per billion (ppb) concentration level, typical of biomedical applications, have been investigated. The laser was operated at liquid nitrogen temperature near lambda = 5.2 microns. In the first configuration, a 100 m optical path length multi-pass cell was employed to enhance the NO absorption. In the second configuration, a technique based on cavity-enhanced spectroscopy (CES) was utilized, with an effective path length of 670 m. Both sensors enabled simultaneous analysis of NO and CO2 concentrations in exhaled air. The minimum detectable NO concentration was found to be 3 ppb with a multi-pass cell and 16 ppb when using CES. The two techniques are compared, and potential future developments are discussed.

NASA Discipline Environmental Health↗

Soil Temperature and Moisture within the Kougarok Fire Complex, Kougarok Road Mile Marker 86, Seward Peninsula, Alaska, 2019-2023

Daily averages of soil temperature and moisture measured once every hour at different heights located at Intensive Monitoring Stations within the Kougarok Fire Complex, Kougarok Road Mile Marker 86 site. Data were retrieved annually from 2019-2023. Package contains 21 *.CSV data files plus a file level metadata *.CSV, data dictionary *.CSV, data file inventory *.CSV, and sensor location site map *.JPG. Data files have header rows, NaN fields indicate invalid or missing data, and negative vertical offsets are above ground.The Kougarok tundra fire complex (KFC) is located north of Nome and the Kigluaik Mountains, near Quartz Creek and the Kougarok River. The site is accessed by foot from the end of the Nome-Taylor Highway (mile marker 86; also called the Kougarok or Beam Road). The KFC burned in six major fires in the decades since 1950 (Alaska Interagency Coordination Center, unpublished data). Lightning ignited five of these fires (1971, 1997, 2015, and 2019) and one was human caused (2002). The mosaic of overlapping fire scars allows for the study of repeat fires in the tundra which, until recently, was not a common phenomenon outside the boreal forest in Alaska. Our reference unburned tundra fire site is south of the KFC located at mile marker 80 of the Nome-Taylor Highway.The two most recent fires are the Mingvk Lake (2015; 21,698 acres burned from 7/27/2015 to 9/28/2015) and Garfield Creek (2019; 422 acres burned from 7/31/2019 to 8/20/19). The Mingvk Lake fire scar includes areas that burned 1-4x (1971, 1997, 2002), while the entirety of the Garfield Creek fire scar has burned 2x previously (1971, 2002).Previous research at the KFC focused on permafrost (Liljedahl et al. 2007; Narita et al. 2015; Iwahana et al. 2016; Tsuyuzaki, Iwahana, and Saito 2017) and vegetation (Narita et al. 2015; Hollingsworth et al. 2021) response to fire. The central Seward Peninsula is characterized by continuous permafrost with a thickness of 15 to 30 m and a mean active layer thickness of 56 cm (Hinzman et al. 2003). Sloping hills with mixed shrub–tussock tundra and tussock tundra vegetation in the uplands are characteristic of the region. Three micrometeorological towers near the Kougarok field site recorded a mean annual temperature of −2.4°C, mean January temperature of −23.1°C, mean July temperature of +11°C, and mean summer rainfall (June–August) of 94 mm from 2000 to 2006 (Liljedahl et al. 2007).The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Compact instantaneous phase-shifting Sagnac interferometer for nanoscale tilt measurement

There is a large inter disciplinary need for high precision angular sensors in state-of-the-art technologies. This particularly applies to optical metrology in the field of Extreme Ultraviolet (EUV) or X-ray where the optics are commonly characterized in terms of slope errors at a nanoradian scale level. Here, in this paper, we present the in-lab development of an angular sensor based on a single reflection compact Sagnac interferometer using instantaneous phase shifting. Our automated prototype and first results show less than 15 nrad RMS repeatability, at least 200 nrad sensitivity and 280 nrad reproducibility over 100 μrad angular range.

36 MATERIALS SCIENCE↗

Smart Medical Systems with Application to Nutrition and Fitness in Space

Smart medical systems are being developed to allow medical treatments to address alterations in chemical and physiological status in real time. In a smart medical system sensor arrays assess subject status, which are interpreted by computer processors which analyze multiple inputs and recommend treatment interventions. The response of the subject to the treatment is again assessed by the sensor arrays, closing the loop. An early form of "smart medicine" has been practiced in space to assess nutrition. Nutrient levels are assessed with food frequency questionnaires, which are interpreted by flight surgeons to recommend in-flight alterations in diet. In the future, sensor arrays will directly probe body chemistry. Near infrared spectroscopy can be used to noninvasively measure several blood and tissue parameters which are important in the assessment of nutrition and fitness. In particular, this technology can be used to measure blood hematocrit and interstitial fluid pH. The noninvasive measurement of interstitial pH is discussed as a surrogate for blood lactate measurement for the development and real-time assessment of exercise protocols in space. Earth-based application of these sensors are also described.

Soller, Babs R.↗

RF Energy Harvesting Peel-and-Stick Sensors

PARC, a Xerox Company, is developing a low-cost system of peel-and-stick wireless sensors that will enable widespread building environment sensor deployment with the potential to deliver up to 30% energy savings. The system is embodied by a set of RF hubs that provide power to the automatically located sensor nodes, and relays data wirelessly to the building management system (BMS). The sensor nodes are flexible electronic labels powered by rectified RF energy transmitted by a RF hub and can contain multiple printed and conventional sensors. The system design overcomes limitations in wireless sensors related to power delivery, lifetime, and cost by eliminating batteries and photovoltaic devices. The sensor localization is performed automatically by the inclusion of a programmable multidirectional antenna array in the RF hub. Comparison of signal strengths when the RF beam is swept allows for sensor localization, further reducing installation effort and enabling automatic recommissioning of sensors that have been relocated, overcoming a significant challenge in building operations. PARC has already demonstrated wireless power and temperature data transmission up to a distance of 20m with a duty cycle less than a minute between measurements, using power levels well within the FCC regulation limits in the 902-928 MHz ISM band. The sensor’s RF energy harvesting antenna dimensions was less than 5cmx9cm, demonstrating the possibility of small form factor for the sensor nodes.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Angular and Polarization Response of Multimode Sensors with Resistive-Grid Absorbers

High sensitivity receiver systems with near ideal polarization sensitivity are highly desirable for development of millimeter and sub-millimeter radio astronomy. Multimoded bolometers provide a unique solution to achieve such sensitivity, for which hundreds of single-mode sensors would otherwise be required. The primary concern in employing such multimoded sensors for polarimetery is the control of the polarization systematics. In this paper, we examine the angular- and polarization- dependent absorption pattern of a thin resistive grid or membrane, which models an absorber used for a multimoded bolometer. The result shows that a freestanding thin resistive absorber with a surface resistivity of eta/2, where eta is the impedance of free space, attains a beam pattern with equal E- and H-plane responses, leading to zero cross polarization. For a resistive-grid absorber, the condition is met when a pair of grids is positioned orthogonal to each other and both have a resistivity of eta/2. When a reflective backshort termination is employed to improve absorption efficiency, the cross-polar level can be suppressed below −30 dB if acceptance angle of the sensor is limited to < or approx. 60deg. The small cross-polar systematics have even-parity patterns and do not contaminate the measurements of odd-parity polarization patterns, for which many of recent instruments for cosmic microwave background are designed. Underlying symmetry that suppresses these cross-polar systematics is discussed in detail. The estimates and formalism provided in this paper offer key tools in the design consideration of the instruments using the multimoded polarimeters.

High sensitivity receiver systems↗

Evaluation of a fault tolerant system for an integrated avionics sensor configuration with TSRV flight data

The performance analysis results of a fault inferring nonlinear detection system (FINDS) using sensor flight data for the NASA ATOPS B-737 aircraft in a Microwave Landing System (MLS) environment is presented. First, a statistical analysis of the flight recorded sensor data was made in order to determine the characteristics of sensor inaccuracies. Next, modifications were made to the detection and decision functions in the FINDS algorithm in order to improve false alarm and failure detection performance under real modelling errors present in the flight data. Finally, the failure detection and false alarm performance of the FINDS algorithm were analyzed by injecting bias failures into fourteen sensor outputs over six repetitive runs of the five minute flight data. In general, the detection speed, failure level estimation, and false alarm performance showed a marked improvement over the previously reported simulation runs. In agreement with earlier results, detection speed was faster for filter measurement sensors soon as MLS than for filter input sensors such as flight control accelerometers.

Caglayan, A. K.↗

Piezoresistive stress sensors on (110) silicon wafers

Structural reliability of electronic packages has become an increasing concern for a variety of reasons including the advent of higher integrated circuit densities, power density levels, and operating temperatures. A powerful method for experimental evaluation of die stress distributions is the use of test chips incorporating integral piezoresistive sensors. In this paper, the basic equations needed for the design of stress sensors fabricated on the surface of (110) oriented silicon wafers have been presented. Several sensor rosette configurations have been explored, including the familiar three-element 0-45-90 rosette. Rosette designs have been found which minimize the necessary calibration procedures and permit more stress components to be measured. It has been established that stress sensors on the surface of (110) test chips are sensitive to four out of the six stress components at a point.

Kang, Y. L.↗

Microreactor Optimization Using Simulation And Economics (mouse)

Microreactor Optimization Using Simulation and Economics (MOUSE) is a tool that integrates both nuclear microreactor design and reactor economics to provide comprehensive evaluations and optimizations. This tool enables stakeholders to explore the interplay between technical and economic variables, guiding them towards effective and competitive microreactor solutions. For the reactor core simulations, MOUSE leverages the OpenMC Monte Carlo Particle Transport Code to perform detailed core simulations for various microreactor designs. The included OpenMC models are 2D core designs of a Liquid Metal Thermal Microreactor (LMTR), a Gas-Cooled TRISO-Fueled Microreactor (GCMR), and a Heat Pipe Microreactor. Beyond core design, MOUSE includes simplified calculations for: - Calculating the masses of heat exchangers within the system. - Mechanical power of pumps. - Estimating the area occupied by various buildings within the nuclear plant. For the economic analysis, MOUSE provides detailed bottom-up cost estimates, encompassing a wide range of costs including preconstruction costs, direct costs, indirect costs, training costs, financial costs, operation & maintenance (O&M) costs, and fuel costs. These cost estimations are developed using data from the MARVEL project and additional literature sources, enabling the calculation of total capital costs and levelized cost of energy for both first-of-a-kind and nth-of-a-kind microreactors. MOUSE also enables analysis of the cost drivers and competitiveness in the electricity market. MOUSE allows users to modify a wide array of technical and economic parameters to evaluate different scenarios and their impacts. Examples of these parameters include: Fuels, coolants, or reflector materials Enrichment levels Control drum materials and geometry Fuel pin geometry and materials Moderator pin geometry and materials Reactor core and reflector dimensions Packing factor for the TRISO particles Nuclear reactor power and reactor burnup Number of sensors Shielding thickness Reactor vessel and guard vessel dimensions Operational staff requirements Number of emergency shutdowns Levelization period Interest rate Construction duration Since MOUSE is powered by the WATTS toolkit, it supports optimization studies, parametric analyses, and uncertainty calculations/propagation. The optimization techniques enable users to identify optimal design and economic configurations. The parametric analysis tools allow users to explore the sensitivity of various parameters, while uncertainty propagation helps quantify the impact of uncertainties on overall performance and cost. User Interface and Workflow: Currently, MOUSE is a command-line-based tool. Users can input various reactor design or economic parameters, modify the designs, run simulations, and visualize results through comprehensive data visualization and reporting capabilities. The typical workflow involves setting up the reactor model, defining economic parameters, running simulations, and analyzing the results to make informed decisions. By combining advanced design calculations with detailed economic modeling, MOUSE provides a robust framework for optimizing nuclear microreactor technologies, enhancing their competitiveness, and guiding stakeholders towards innovative and cost-effective solutions.

Hanna, Botros [Idaho National Laboratory (INL), Id↗

Measuring Moisture Levels in Graphite Epoxy Composite Sandwich Structures

Graphite epoxy composite (GEC) materials are used in the construction of rocket fairings, nose cones, interstage adapters, and heat shields due to their high strength and light weight. However, they absorb moisture depending on the environmental conditions they are exposed to prior to launch. Too much moisture absorption can become a problem when temperature and pressure changes experienced during launch cause the water to vaporize. The rapid state change of the water can result in structural failure of the material. In addition, heat and moisture combine to weaken GEC structures. Diffusion models that predict the total accumulated moisture content based on the environmental conditions are one accepted method of determining if the material strength has been reduced to an unacceptable level. However, there currently doesn t exist any field measurement technique to estimate the actual moisture content of a composite structure. A multi-layer diffusion model was constructed with Mathematica to predict moisture absorption and desorption from the GEC sandwich structure. This model is used in conjunction with relative humidity/temperature sensors both on the inside and outside of the material to determine the moisture levels in the structure. Because the core materials have much higher diffusivity than the face sheets, a single relative humidity measurement will accurately reflect the moisture levels in the core. When combined with an external relative humidity measurement, the model can be used to determine the moisture levels in the face sheets. Since diffusion is temperaturedependent, the temperature measurements are used to determine the diffusivity of the face sheets for the model computations.

Nurge, Mark↗

Development, construction and tests of the Mu2e electromagnetic calorimeter mechanical structures

The “muon-to-electron conversion” (Mu2e) experiment at Fermilab will search for the charged lepton flavour violating neutrino-less coherent conversion of a muon into an electron in the field of an aluminum nucleus. The observation of this process would be the unambiguous evidence of the existence of physics beyond the standard model. Mu2e detectors comprise a straw-tracker, an electromagnetic calorimeter and an external veto for cosmic rays. In particular, the calorimeter provides excellent electron identification, a fast calorimetric online trigger, and complementary information to aid pattern recognition and track reconstruction. The detector has been designed as a state-of-the-art crystal calorimeter and employs 1348 pure Cesium Iodide (CsI) crystals readout by UV-extended silicon photosensors and fast front-end and digitization electronics. A design consisting of two identical annular matrices (named “disks”) positioned at the relative distance of 70 cm downstream the aluminum target along the muon beamline satisfies the Mu2e physics requirements. The hostile Mu2e operational conditions, in terms of radiation levels (total expected ionizing dose of 12 krad and a neutron fluence of 5 × 10$^{10}$ n/cm$^{2}$ @ 1 MeV$_{eq}$ (Si)/y), magnetic field intensity (1 T) and vacuum level (10$^{-4}$ Torr) have posed tight constraints on scintillating materials, sensors, electronics and on the design of the detector mechanical structures and material choice. The support structure of each 674 crystal matrix is composed of an aluminum hollow ring and parts made of open-cell vacuum-compatible carbon fiber. The photosensors and front-end electronics for the readout of each crystal are inserted in a machined copper holder and make a unique mechanical unit. The resulting 674 mechanical units are supported by a machined plate of vacuum-compatible plastic material. The plate also integrates the cooling system made of a network of copper lines flowing a low temperature radiation-hard fluid and placed in thermal contact with the copper holders to constitute a low resistance thermal bridge. The data acquisition electronics are hosted in aluminum custom crates positioned on the external lateral surface of the disks. The crates also integrate the electronics cooling system as lines running in parallel to the front-end system. In this paper we report on the calorimeter mechanical structure design, the mechanical and thermal simulations that have determined the design technological choices, and the status of component production, quality assurance tests and plans for assembly at Fermilab.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Adaptive Self Tuning

The AST software includes numeric methods to 1) adjust STA/LTA signal detector trigger level (TL) values and 2) filter detections for a network of sensors. AST adapts TL values to the current state of the environment by leveraging cooperation within a neighborhood of sensors. The key metric that guides the dynamic tuning is consistency of each sensor with its nearest neighbors: TL values are automatically adjusted on a per station basis to be more or less sensitive to produce consistent agreement of detections in its neighborhood. The AST algorithm adapts in near real-time to changing conditions in an attempt to automatically self-tune a signal detector to identify (detect) only signals from events of interest.

Peterson, Matthew↗

Agricultural Burning and Air Quality over Northern India: A Synergistic Analysis using NASA’s A-train Satellite Data and Ground Measurements

In recent years, New Delhi, the capital city of India, has been ranked among the most polluted cities in the world in terms of air quality related to Particulate Matter (PM). In this paper, we show that the PM 2.5 , a major component of the measure of air quality, over New Delhi is strongly impacted by the agricultural fires in the north-western Indian states of Punjab and Haryana during the post-monsoon season (October and November). Using data from NASA’s A-train sensors (MODIS, OMI, and CALIOP) in conjunction with the ground-level PM 2.5 mass concentration measured at the US Embassy site in New Delhi (2013-2015), and back-trajectory analysis, it is shown that the smoke particles produced from numerous fires in Punjab and Haryana are advected over the national capital region under the influence of north-westerly winds, thereby greatly increasing the ground-level PM 2.5 from 50 µg/m -3 before the onset of burning in early October to as high as 300 µg/m -3 (24-hour averaged, 7-day running mean) during the peak burning period in early November. Relationships between the satellite measurements of fire counts and aerosols in the crop burning region and PM 2.5 over New Delhi are found to be strongly correlated. A linear regression analysis reveals that increments in the fire counts, aerosol optical depth, UV Aerosol Index, and aerosol absorption optical depth by 100, 1.0, 1.0, and 0.1 over the burning areas result in an increase in PM 2.5 by 20, 47, 134, and 142 µg/m -3 , respectively, over New Delhi. Back-trajectory analysis shows that about 74%, 62%, and 51% of total number of daily trajectories for altitudes 10 m, 500 m, and 1500 m, respectively, intercepted the biomass burning region before arriving at the receptor location in New Delhi; this further corroborates the transport mechanism observed in the satellite data. A 15-year long record of A-train satellite measurements shows an increasing number of agricultural fires (29% higher) and aerosol loading during the second half of the record (2009-2015) compared to that during the first half (2002-2008), which is one of the major concerns for the deteriorating air quality in the region.

54 ENVIRONMENTAL SCIENCES↗

The Rome-GSFC magnetic field experiment for Helios A and B /E 3/

The Rome-GSFC magnetic field experiment utilizes a triaxial fluxgate (saturable inductor) magnetometer. The sensor unit is mounted on the end of a boom approximately four meters from the spacecraft spin axis. The three analog outputs of the magnetometer are converted into three 9 bit digital words. The experiment utilizes an automatic inflight range switch to select the optimum dynamic range out of 4 ranges. A nonmagnetic thermally oscillating actuator is used to reorient the sensor unit by 90 deg to determine all three axes zero levels. The accuracy should be approximately plus or minus 0.1 gamma. The vector measurements are made at equal intervals in time ranging from 16 per second down to 1 per second depending on the telemetry bit rate.

Scearce, C.↗

A program for calculating load coefficient matrices utilizing the force summation method, L218 (LOADS). Volume 2: Supplemental system design and maintenance document

The LOADS computer program L218 which calculates dynamic load coefficient matrices utilizing the force summation method is described. The load equations are derived for a flight vehicle in straight and level flight and excited by gusts and/or control motions. In addition, sensor equations are calculated for use with an active control system. The load coefficient matrices are calculated for the following types of loads: (1) translational and rotational accelerations, velocities, and displacements; (2) panel aerodynamic forces; (3) net panel forces; and (4) shears, bending moments, and torsions.

Anderson, L. R.↗

Modulation of centimetric waves by long gravity waves - Progress report on field and laboratory results

Results are presented from laboratory and field experiments on the modulation of short waves by long waves. The field study employed a wave follower capable of tracking ocean waves with frequencies less than 1.0 Hz and heights less than 2.0 m. A high-response laser-optical system was used to detect upwind-downwind and cross-wind slopes of short waves. The laboratory study was conducted with wind over periodic long waves. The laboratory findings are discussed and compared with laboratory radar measurements and also short wave measurements obtained in the field. It is found that long waves significantly modulate the short wave dispersion by their orbital velocity, and that demodulation is necessary if the data collected by remote sensors are to be compared to surface penetrating devices. The modulation level is weak for wavelengths in the range 2.76-3.30 cm. Other relevant results are also presented.

Shemdin, O. H.↗

A program for calculating load coefficient matrices utilizing the force summation method, L218 (LOADS). Volume 1: Engineering and usage

The LOADS program L218, a digital computer program that calculates dynamic load coefficient matrices utilizing the force summation method, is described. The load equations are derived for a flight vehicle in straight and level flight and excited by gusts and/or control motions. In addition, sensor equations are calculated for use with an active control system. The load coefficient matrices are calculated for the following types of loads: translational and rotational accelerations, velocities, and displacements; panel aerodynamic forces; net panel forces; shears and moments. Program usage and a brief description of the analysis used are presented. A description of the design and structure of the program to aid those who will maintain and/or modify the program in the future is included.

Miller, R. D.↗