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At least 955 records · Page 53
All sourcesNASA NTRSDOE OSTI
NASA NTRS1999

Far Ultraviolet Imaging from the Image Spacecraft: System Design - 1

Direct imaging of the magnetosphere by the IMAGE spacecraft A,ill be supplemented by observation of the global aurora, the footprint of magnetospheric regions. To assure the simultaneity of these observations and tile measurement of the magnetospheric back-round neutral gas density, the IMAGE satellite instrument complement includes three Far Ultraviolet (FUV) instruments. In tile wavelength region 120-1 90 nm. a downward-viewing aurora imager is only minimally contaminated sunlight, scattered from clouds and ground, and radiance of the aurora observed in a nadir viewing geometry can be observed in the presence of the high-latitude day-low. Tile Wideband Imaging Camera (WIC) will provide broadband ultraviolet images of the aurora for maximum spatial and temporal resolution by imaging the LBH N2 bands of the aurora. The Spectrographic Imager (SI), a monochromatic imager, will image different types of aurora, filtered by wavelength. By measuring the Doppler-shifted Lyman-alpha, the proton-induced component of the aurora will be imaged separately. Finally, the GEO instrument will observe the distribution of the geocoronal emission, which is a measure of the neutral background density source of the charge exchange in the magnetosphere. The FUV instrument complement looks radially outward from the rotating IMAGE satellite and, therefore, it spends only a short time observing tile aurora and the Earth during, each spin. Detailed descriptions of the WIC, Si, GEO, and their individual performance validations are discussed in companion papers. This paper summarizes the system requirements and system design approach taken to satisfy the science requirements. One primary requirement is to maximize photon collection efficiency and use efficiently tile short time available foe exposures. The FUV auroral imagers WIC and SI both have wide fields of view and take data continuously as the auroral region proceeds through the field of view. To minimize data volume, multiple images are taken and electronically co-added by suitably shifting each image to compensate for the spacecraft rotation. In order to minimize resolution loss, the images have to be distortion-corrected in real time foe both WIC and Si prior to co-adding. The distortion correction is accomplished using high-speed look Lip tables that are pre-generated by least square fitting to polynomial functions by the on-orbit processor. The instruments were calibrated individually while on stationary platforms, mostly in vacuum chambers as described in the companion papers. Extensive -round-based testing was performed with visible and near UV simulators mounted oil a rotating platform to estimate their on-orbit performance. The predicted instrument system performance is summarized and some of the preliminary data formats are shown.

Mende, S. B.↗
DOE OSTI2023

Predicting future well performance for environmental remediation design using deep learning

Here in this study, we developed a deep learning (DL) framework with a multi-channel three-dimensional convolutional neural network (MC3D-CNN) to predict well performance and thereby assist future environmental remediation design. Such prediction of extraction well performance at designated locations is critical for configuring pump-and-treat (P&T) well network design and operation, setting reasonable target closure dates for overall remedying, and estimating remedy costs. The framework is developed with operational and monitoring data routinely collected during P&T remedy operations, including well extraction and injection rates as well as in situ contaminant concentrations. Traditionally, the collected data were rarely used for purposes other than assessing past well performance and the accuracy of the conceptual site model. However, recent advances in data-driven computational approaches enable better use of the large datasets to inform future well performance, enhance site characterization, and improve remediation planning. In this study, we established a DL framework to integrate transient three-dimensional contaminant plumes and multiple aquifer properties (e.g., hydraulic conductivity and hydrostratigraphic maps) to identify characteristic patterns controlling and representing extraction well mass recovery, aiming at providing future mass recovery estimates for existing wells and candidate wells at any proposed locations. We evaluated our framework by using a realistic synthetic dataset generated from a well-calibrated flow and transport model used in the 200 West Area of the U.S. Department of Energy’s Hanford Site in southeastern Washington state. The multi-channel feature in our framework allows integration of various types and temporal densities of training datasets for DL model development. Overall, we found that the trained DL model achieved an accuracy of over 90% in ranking extraction well performance in validation datasets, and over 80% in predicting high-performance-ranking well locations. This data-informed approach provides a flexible tool to support adaptive site management, streamline decision-making, and potentially reduce remediation time and costs. Our DL framework can be used as a filtering tool to improve the current P&T network optimization design by reducing the number of candidate well locations.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1995

3DGRAPE/AL User's Manual

This document is a users' manual for a new three-dimensional structured multiple-block volume g generator called 3DGRAPE/AL. It is a significantly improved version of the previously-released a widely-distributed programs 3DGRAPE and 3DMAGGS. It generates volume grids by iteratively solving the Poisson Equations in three-dimensions. The right-hand-side terms are designed so that user-specific; grid cell heights and user-specified grid cell skewness near boundary surfaces result automatically, with little user intervention. The code is written in Fortran-77, and can be installed with or without a simple graphical user interface which allows the user to watch as the grid is generated. An introduction describing the improvements over the antecedent 3DGRAPE code is presented first. Then follows a chapter on the basic grid generator program itself, and comments on installing it. The input is then described in detail. After that is a description of the Graphical User Interface. Five example cases are shown next, with plots of the results. Following that is a chapter on two input filters which allow use of input data generated elsewhere. Last is a treatment of the theory embodied in the code.

Sorenson, Reese L.↗
NASA NTRS1997

A Novel, Poly-Etalon, Fabry-Perot for Planetary Research

In an effort to develop a mechanically robust, high throughput and solid state spectrometer several liquid crystal Fabry-Perot etalons were constructed. The etalons were tested for spectral response, radiation resistance and optical transmission. The first year of this project was spent developing and understanding the properties of the liquid crystal etalons; in the second year an intensified all-sky imaging system was developed around a pair of LC etalons. The imaging system, developed jointly with SRI International represents a unique brassboard to demonstrate the use of LC etalons as tunable filters. The first set of etalons constructed in year one of this project were tested for spectral response and throughput while etalon surrogates were exposed to proton radiation simulating the exposure of an object in Low Earth Orbit (LEO). The 2" diameter etalons had a measure finesse of approximately 10 and were tunable over five orders. Liquid crystals exposed to proton irradiation showed no signs of damage. In year two two larger diameter (3") etalons were constructed with gaps of 3 and 5 microns. This pair of etalons is for use in a high resolution, all-sky spectral imager. The WATUMI imager system follows the heritage of all sky, narrow band, intensified imagers however it includes two LC Fabry-Perot etalons to provide tunability and the ability to switch wavelengths rapidly, an import consideration in auroral airglow imaging. This work also resulted in two publications and one poster presentation. The instrument will be uniquely capable, with superior throughput and speed, to measure optical airglow of multiple emission lines in harsh conditions.

Kerr, Robert B.↗
NASA NTRS2007

NASA Tech Briefs, January 2007

Topics covered include: Flexible Skins Containing Integrated Sensors and Circuitry; Artificial Hair Cells for Sensing Flows; Video Guidance Sensor and Time-of-Flight Rangefinder; Optical Beam-Shear Sensors; Multiple-Agent Air/Ground Autonomous Exploration Systems; A 640 512-Pixel Portable Long-Wavelength Infrared Camera; An Array of Optical Receivers for Deep-Space Communications; Microstrip Antenna Arrays on Multilayer LCP Substrates; Applications for Subvocal Speech; Multiloop Rapid-Rise/Rapid Fall High-Voltage Power Supply; The PICWidget; Fusing Symbolic and Numerical Diagnostic Computations; Probabilistic Reasoning for Robustness in Automated Planning; Short-Term Forecasting of Radiation Belt and Ring Current; JMS Proxy and C/C++ Client SDK; XML Flight/Ground Data Dictionary Management; Cross-Compiler for Modeling Space-Flight Systems; Composite Elastic Skins for Shape-Changing Structures; Glass/Ceramic Composites for Sealing Solid Oxide Fuel Cells; Aligning Optical Fibers by Means of Actuated MEMS Wedges; Manufacturing Large Membrane Mirrors at Low Cost; Double-Vacuum-Bag Process for Making Resin- Matrix Composites; Surface Bacterial-Spore Assay Using Tb3+/DPA Luminescence; Simplified Microarray Technique for Identifying mRNA in Rare Samples; High-Resolution, Wide-Field-of-View Scanning Telescope; Multispectral Imager With Improved Filter Wheel and Optics; Integral Radiator and Storage Tank; Compensation for Phase Anisotropy of a Metal Reflector; Optical Characterization of Molecular Contaminant Films; Integrated Hardware and Software for No-Loss Computing; Decision-Tree Formulation With Order-1 Lateral Execution; GIS Methodology for Planning Planetary-Rover Operations; Optimal Calibration of the Spitzer Space Telescope; Automated Detection of Events of Scientific Interest; Representation-Independent Iteration of Sparse Data Arrays; Mission Operations of the Mars Exploration Rovers; and More About Software for No-Loss Computing.

Source record↗
NASA NTRS2011

Attitude Estimation in Fractionated Spacecraft Cluster Systems

An attitude estimation was examined in fractioned free-flying spacecraft. Instead of a single, monolithic spacecraft, a fractionated free-flying spacecraft uses multiple spacecraft modules. These modules are connected only through wireless communication links and, potentially, wireless power links. The key advantage of this concept is the ability to respond to uncertainty. For example, if a single spacecraft module in the cluster fails, a new one can be launched at a lower cost and risk than would be incurred with onorbit servicing or replacement of the monolithic spacecraft. In order to create such a system, however, it is essential to know what the navigation capabilities of the fractionated system are as a function of the capabilities of the individual modules, and to have an algorithm that can perform estimation of the attitudes and relative positions of the modules with fractionated sensing capabilities. Looking specifically at fractionated attitude estimation with startrackers and optical relative attitude sensors, a set of mathematical tools has been developed that specify the set of sensors necessary to ensure that the attitude of the entire cluster ( cluster attitude ) can be observed. Also developed was a navigation filter that can estimate the cluster attitude if these conditions are satisfied. Each module in the cluster may have either a startracker, a relative attitude sensor, or both. An extended Kalman filter can be used to estimate the attitude of all modules. A range of estimation performances can be achieved depending on the sensors used and the topology of the sensing network.

Hadaegh, Fred Y.↗
NASA NTRS2009

Satellite-Scale Snow Water Equivalent Assimilation into a High-Resolution Land Surface Model

An ensemble Kalman filter (EnKF) is used in a suite of synthetic experiments to assimilate coarse-scale (25 km) snow water equivalent (SWE) observations (typical of satellite retrievals) into fine-scale (1 km) model simulations. Coarse-scale observations are assimilated directly using an observation operator for mapping between the coarse and fine scales or, alternatively, after disaggregation (re-gridding) to the fine-scale model resolution prior to data assimilation. In either case observations are assimilated either simultaneously or independently for each location. Results indicate that assimilating disaggregated fine-scale observations independently (method 1D-F1) is less efficient than assimilating a collection of neighboring disaggregated observations (method 3D-Fm). Direct assimilation of coarse-scale observations is superior to a priori disaggregation. Independent assimilation of individual coarse-scale observations (method 3D-C1) can bring the overall mean analyzed field close to the truth, but does not necessarily improve estimates of the fine-scale structure. There is a clear benefit to simultaneously assimilating multiple coarse-scale observations (method 3D-Cm) even as the entire domain is observed, indicating that underlying spatial error correlations can be exploited to improve SWE estimates. Method 3D-Cm avoids artificial transitions at the coarse observation pixel boundaries and can reduce the RMSE by 60% when compared to the open loop in this study.

De Lannoy, Gabrielle J.M.↗
DOE OSTI2022

Search and identification of transient and variable radio sources using MeerKAT observations: a case study on the MAXI J1820+070 field

ABSTRACT Many transient and variable sources detected at multiple wavelengths are also observed to vary at radio frequencies. However, these samples are typically biased towards sources that are initially detected in wide-field optical, X-ray, or gamma-ray surveys. Many sources that are insufficiently bright at higher frequencies are therefore missed, leading to potential gaps in our knowledge of these sources and missing populations that are not detectable in optical, X-rays, or gamma-rays. Taking advantage of new state-of-the-art radio facilities that provide high-quality wide-field images with fast survey speeds, we can now conduct unbiased surveys for transient and variable sources at radio frequencies. In this paper, we present an unbiased survey using observations obtained by MeerKAT, a mid-frequency (∼GHz) radio array in South Africa’s Karoo Desert. The observations used were obtained as part of a weekly monitoring campaign for X-ray binaries (XRBs) and we focus on the field of MAXI J1820+070. We develop methods to efficiently filter transient and variable candidates that can be directly applied to other data sets. In addition to MAXI J1820+070, we identify four likely active galactic nuclei, one source that could be a Galactic source (pulsar or quiescent XRB) or an AGN, and one variable pulsar. No transient sources, defined as being undetected in deep images, were identified leading to a transient surface density of <3.7 × 10−2 deg−2 at a sensitivity of 1 mJy on time-scales of 1 week at 1.4 GHz.

Rowlinson, A. (ORCID:0000000211957022)↗
NASA NTRS2005

Application of a Constant Gain Extended Kalman Filter for In-Flight Estimation of Aircraft Engine Performance Parameters

An approach based on the Constant Gain Extended Kalman Filter (CGEKF) technique is investigated for the in-flight estimation of non-measurable performance parameters of aircraft engines. Performance parameters, such as thrust and stall margins, provide crucial information for operating an aircraft engine in a safe and efficient manner, but they cannot be directly measured during flight. A technique to accurately estimate these parameters is, therefore, essential for further enhancement of engine operation. In this paper, a CGEKF is developed by combining an on-board engine model and a single Kalman gain matrix. In order to make the on-board engine model adaptive to the real engine s performance variations due to degradation or anomalies, the CGEKF is designed with the ability to adjust its performance through the adjustment of artificial parameters called tuning parameters. With this design approach, the CGEKF can maintain accurate estimation performance when it is applied to aircraft engines at offnominal conditions. The performance of the CGEKF is evaluated in a simulation environment using numerous component degradation and fault scenarios at multiple operating conditions.

Kobayashi, Takahisa↗
NASA NTRS2008

Simulation of Ground Winds Time Series for the NASA Crew Launch Vehicle (CLV)

Simulation of wind time series based on power spectrum density (PSD) and spectral coherence models for ground wind turbulence is described. The wind models, originally developed for the Shuttle program, are based on wind measurements at the NASA 150-m meteorological tower at Cape Canaveral, FL. The current application is for the design and/or protection of the CLV from wind effects during on-pad exposure during periods from as long as days prior to launch, to seconds or minutes just prior to launch and seconds after launch. The evaluation of vehicle response to wind will influence the design and operation of constraint systems for support of the on-pad vehicle. Longitudinal and lateral wind component time series are simulated at critical vehicle locations. The PSD model for wind turbulence is a function of mean wind speed, elevation and temporal frequency. Integration of the PSD equation over a selected frequency range yields the variance of the time series to be simulated. The square root of the PSD defines a low-pass filter that is applied to adjust the components of the Fast Fourier Transform (FFT) of Gaussian white noise. The first simulated time series near the top of the launch vehicle is the inverse transform of the adjusted FFT. Simulation of the wind component time series at the nearest adjacent location (and all other succeeding next nearest locations) is based on a model for the coherence between winds at two locations as a function of frequency and separation distance, where the adjacent locations are separated vertically and/or horizontally. The coherence function is used to calculate a coherence weighted FFT of the wind at the next nearest location, given the FFT of the simulated time series at the previous location and the essentially incoherent FFT of the wind at the selected location derived a priori from the PSD model. The simulated time series at each adjacent location is the inverse Fourier transform of the coherence weighted FFT. For a selected design case, the equations, the process and the simulated time series at multiple vehicle stations are presented.

Adelfang, Stanley I.↗
NASA NTRS2008

The NASA NEESPI Data Portal: Products, Information, and Services

Studies have indicated that land cover and use changes in Northern Eurasia influence global climate system. However, the procedures are not fully understood and it is challenging to understand the interactions between the land changes in this region and the global climate. Having integrated data collections form multiple disciplines are important for studies of climate and environmental changes. Remote sensed and model data are particularly important die to sparse in situ measurements in many Eurasia regions especially in Siberia. The NASA GES DISC (Goddard Earth Sciences Data and Information Services Center) NEESPI data portal has generated infrastructure to provide satellite remote sensing and numerical model data for atmospheric, land surface, and cryosphere. Data searching, subsetting, and downloading functions are available. ONe useful tool is the Web-based online data analysis and visualization system, Giovanni (Goddard Interactive Online Visualization ANd aNalysis Infrastructure), which allows scientists to assess easily the state and dynamics of terrestrial ecosystems in Northern Eurasia and their interactions with global climate system. Recently, we have created a metadata database prototype to expand the NASA NEESPI data portal for providing a venue for NEESPI scientists fo find the desired data easily and leveraging data sharing within NEESPI projects. The database provides product level information. The desired data can be found through navigation and free text search and narrowed down by filtering with a number of constraints. In addition, we have developed a Web Map Service (WMS) prototype to allow access data and images from difference data resources.

Shen, Suhung↗
NASA NTRS2012

Miniaturized Gas Correlation Radiometer for the Detection of Trace Gases in the Martian Atmosphere

We present a miniaturized and simplified version of a gas correlation radiometer (GCR) capable of simultaneously mapping multiple trace gases and identifying active regions on the Mars surface. Gas correlation radiometry (GCR) has been shown to be a sensitive and versatile method for detecting trace gases in Earth's atmosphere. Reduction of the size and mass of the GCR was achieved by implementing compact, light-weight 1 mm inner diameter hollow-core optical fibers (hollow waveguides) as the gas correlation cells. In a comparison with an Earth orbiting CO2 GCR instrument, exchanging the 10 m multipass cells with hollow waveguide gas correlation cells of equivalent path length reduces the mass from approximately 150 kg to approximately 0.5 kg, and reduces the volume from 1.9 m x 1.3 m x 0.86 m to a small bundle of fiber coils approximately 1 meter in diameter by 0.05 m in height (mass and volume reductions of greater than 99%). A unique feature of this instrument is its stackable module design, with a single module for each trace gas. Each of the modules is self-contained, and fundamentally identical; differing by the bandpass filter wavelength range and gas mixtures inside the hollow-waveguide absorption cells. The current configuration contains four stacked modules for simultaneous measurements of methane (CH4), formaldehyde (H2CO), water vapor (H2O), and deuterated water vapor (HDO) but could easily be expanded to include measurements of additional species of interest including nitrous oxide (N2O), hydrogen sulfide (H2S), methanol (CH3OH), and sulfur dioxide (SO2), as well as carbon dioxide (CO2) for a simultaneous measure of mass balance. Preliminary results indicate that a 1 ppb detection limit is possible for both formaldehyde and methane with one second of averaging. Using non-optimized components, we have demonstrated an instrument sensitivity equivalent to approximately 30 ppb for formaldehyde, and approximately 500 ppb for methane. We expect custom bandpass filters and 6 m long waveguides to significantly improve these promising results. Ongoing testing is being conducted on water vapor and deuterated water vapor.

Melroy, Hilary R.↗
DOE OSTI2021

Plasmonically assisted channels of photoemission from metals

We analyze recently measured nonlinear photoemission spectra from Ag surfaces that reveal resonances whose energies do not scale with the applied photon energy but stay pinned to multiples of bulk plasmon energy $\hbar$ω p above the Fermi level. To elucidate these unexpected and peculiar features we investigate the spectra of plasmons generated in a solid by the optically pumped electronic polarization and their effect on photoemission. By combining quadratic response formalism for calculations of photoemission yield, a nonperturbative approach to inelastic electron scattering, and first-principles calculations for the electronic structure, we demonstrate the dependence of probability amplitude for single- and multiplasmon excitations on the basic parameters characterizing the photon pulse and the system. The resulting multiexcitation spectrum evolves towards a truncated plasmonic coherent state. Analogous concept is extrapolated to interpret plasmon generation by multiphoton excited electronic polarization. Based on this we elaborate a scenario that the thus created real plasmons act as supplementary frequency-locked pump field for non-Einsteinian plasmonically assisted channels of photoemission from metals. The established paradigm enables assignment and assessment of the observed linear $\hbar$ω p and nonlinear 2$\hbar$ω p electron yields from Ag. Such effects may be exploited for selective filtering of optical energy conversion in electronic systems.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
NASA NTRS1983

System description

The VAS, carried onboard the GOES 4 and 5 satellites, is a radiometer with 8 visible channel detectors and 6 thermal detectors that detect infrared radiation in 12 spectral bands. A filter wheel in front of the detector is used to achieve the spectral selection. The spatial resolution is 0.9 kilometer in the visible and 7 or 14 kilometers in the infrared depending on the detector used. Full Earth disk coverage is accomplished by spinning in the west to east direction at 100 rpm and by stepping a scan mirror from north to south. Additional VAS instrument characteristics are summarized. The VAS has three operating modes: the operational VISSR mode, the multispectral imaging (MSI) mode, and the dwell sounding (DS) mode. The operational VISSR mode is used by NOAA/NESS for its operational products, which include a visible picture and an 11 micrometer infrared (channel 8) picture at half hour intervals. The other modes are VAS unique. The MSI mode combines the operational VISSR capability (visible plus infrared window) with two additional spectral channels to provide half hourly full Earth disk imagery of atmospheric water vapor, temperature, and cloud distribution. The DS mode is used primarily for sounding to obtain the temperature and moisture profiles. In this mode, multiple spins on the same scan line in a given band are averaged to obtain the required signal to noise ratio for sounding.

Montgomery, H.↗
NASA NTRS1999

Investigation of Next-Generation Earth Radiation Budget Radiometry

The current effort addresses two issues important to the research conducted by the Thermal Radiation Group at Virginia Tech. The first research topic involves the development of a method which can properly model the diffraction of radiation as it enters an instrument aperture. The second topic involves the study of a potential next-generation space-borne radiometric instrument concept. Presented are multiple modeling efforts to describe the diffraction of monochromatic radiant energy passing through an aperture for use in the Monte-Carlo ray-trace environment. Described in detail is a deterministic model based upon Heisenberg's uncertainty principle and the particle theory of light. This method is applicable to either Fraunhofer or Fresnel diffraction situations, but is incapable of predicting the secondary fringes in a diffraction pattern. Also presented is a second diffraction model, based on the Huygens-Fresnel principle with a correcting obliquity factor. This model is useful for predicting Fraunhofer diffraction, and can predict the secondary fringes because it keeps track of phase. NASA is planning for the next-generation of instruments to follow CERES (Clouds and the Earth's Radiant Energy System), an instrument which measures components of the Earth's radiant energy budget in three spectral bands. A potential next-generation concept involves modification of the current CERES instrument to measure in a larger number of wavelength bands. This increased spectral partitioning would be achieved by the addition of filters and detectors to the current CERES geometry. The capacity of the CERES telescope to serve for this purpose is addressed in this thesis.

Coffey, Katherine L.↗
NASA NTRS1971

Avalanche-diode oscillator circuit with tuning at multiple frequencies

Detailed theoretical analysis of three different modes or types of high efficiency oscillation in a PIN diode are presented. For the TRAPATT mode in a PIN diode, it is shown that a traveling avalanche zone is not necessary to generate a dense trapped plasma. An economical computer program for TRAPATT oscillations in a PIN diode is described. Typical results of diode power, dc-to-RF conversion efficiency, and required circuit impedances are presented for several different current waveforms. A semianalytical solution for a second type of high efficiency mode in a PIN diode is derived assuming a rectangular current waveform. A quasi-static approximation is employed to derive a semianalytical solution for the voltage across a PIN diode in a third mode, where avalanching occurs during a major portion of a half cycle. Calculations for this mode indicate that the power increases proportionally to the magnitude of the drive current with a small decrease in efficiency relative to the ordinary TRAPATT mode. An analytical solution is also given for a PIN diode, where it is assumed that the ionization coefficient is a step function. It is shown that the step-ionization approximation permits one to draw possible patterns of avalanche region in the depletion layer as a function of time. A rule governing admissible patterns is derived and an example solution given for one admissible pattern. Preliminary experimental results on the high-efficiency oscillations are presented and discussed. Two different experimental circuits, which used channel-dropping filters to provide independent harmonic tuning, are described. Simpler circuits used to produce high-efficiency oscillations are discussed. Results of experiments using inexpensive Fairchild FD300 diodes are given.

Parker, D.↗
NASA NTRS2007

Improved Underwater Excitation-Emission Matrix Fluorometer

A compact, high-resolution, two-dimensional excitation-emission matrix fluorometer (EEMF) has been designed and built specifically for use in identifying and measuring the concentrations of organic compounds, including polluting hydrocarbons, in natural underwater settings. Heretofore, most EEMFs have been designed and built for installation in laboratories, where they are used to analyze the contents of samples collected in the field and brought to the laboratories. Because the present EEMF can be operated in the field, it is better suited to measurement of spatially and temporally varying concentrations of substances of interest. In excitation-emission matrix (EEM) fluorometry, fluorescence is excited by irradiating a sample at one or more wavelengths, and the fluorescent emission from the sample is measured at multiple wavelengths. When excitation is provided at only one wavelength, the technique is termed one-dimensional (1D) EEM fluorometry because the resulting matrix of fluorescence emission data (the EEM) contains only one row or column. When excitation is provided at multiple wavelengths, the technique is termed two-dimensional (2D) EEM fluorometry because the resulting EEM contains multiple rows and columns. EEM fluorometry - especially the 2D variety - is well established as a means of simultaneously detecting numerous dissolved and particulate compounds in water. Each compound or pool of compounds has a unique spectral fluorescence signature, and each EEM is rich in information content, in that it can contain multiple fluorescence signatures. By use of deconvolution and/or other mixture-analyses techniques, it is often possible to isolate the spectral signature of compounds of interest, even when their fluorescence spectra overlap. What distinguishes the present 2D EEMF over prior laboratory-type 2D EEMFs are several improvements in packaging (including a sealed housing) and other aspects of design that render it suitable for use in natural underwater settings. In addition, the design of the present 2D EEMF incorporates improvements over the one prior commercial underwater 2D EEMF, developed in 1994 by the same company that developed the present one. Notable advanced features of the present EEMF include the following: 1) High sensitivity and spectral resolution are achieved by use of an off-the-shelf grating spectrometer equipped with a sensor in the form of a commercial astronomical- grade 256 532-pixel charge-coupled-device (CCD) array. 2) All of the power supply, timing, control, and readout circuits for the illumination source and the CCD, ancillary environmental monitoring sensors, and circuitry for controlling a shutter or filter motor are custom-designed and mounted compactly on three circuit boards below a fourth circuit board that holds the CCD (see figure). 3) The compactness of the grating spectrometer, CCD, and circuit assembly makes it possible to fit the entire instrument into a compact package that is intended to be maneuverable underwater by one person. 4) In mass production, the cost of the complete instrument would be relatively low - estimated at approximately $30,000 at 2005 prices.

Moore, Casey↗
DOE OSTI2023

Genomic variation across Chinook salmon populations reveals effects of a duplication on migration alleles and supports fine scale structure

Abstract The distribution of ecotypic variation in natural populations is influenced by neutral and adaptive evolutionary forces that are challenging to disentangle. This study provides a high‐resolution portrait of genomic variation in Chinook salmon ( Oncorhynchus tshawytscha ) with emphasis on a region of major effect for ecotypic variation in migration timing. With a filtered data set of ~13 million single nucleotide polymorphisms (SNPs) from low‐coverage whole genome resequencing of 53 populations (3566 barcoded individuals), we contrasted patterns of genomic structure within and among major lineages and examined the extent of a selective sweep at a major effect region underlying migration timing (GREB1L/ROCK1). Neutral variation provided support for fine‐scale structure of populations, while allele frequency variation in GREB1L/ROCK1 was highly correlated with mean return timing for early and late migrating populations within each of the lineages ( r 2 = .58–.95; p < .001). However, the extent of selection within the genomic region controlling migration timing was much narrower in one lineage (interior stream‐type) compared to the other two major lineages, which corresponded to the breadth of phenotypic variation in migration timing observed among lineages. Evidence of a duplicated block within GREB1L/ROCK1 may be responsible for reduced recombination in this portion of the genome and contributes to phenotypic variation within and across lineages. Lastly, SNP positions across GREB1L/ROCK1 were assessed for their utility in discriminating migration timing among lineages, and we recommend multiple markers nearest the duplication to provide highest accuracy in conservation applications such as those that aim to protect early migrating Chinook salmon. These results highlight the need to investigate variation throughout the genome and the effects of structural variants on ecologically relevant phenotypic variation in natural species.

Horn, Rebekah L.↗