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DOE OSTI2022

The Complete Genome Sequence and Structure of the Oleaginous Rhodococcus opacus Strain PD630 Through Nanopore Technology

Rhodococcus bacterial strains are characterized by wide metabolic versatility and extraordinary resistance to environmental stresses. The high versatility and adaptability of Rhodococcus strains is partly related with large and complex genomes (up to 10.1 Mbp) including high genetic redundancy and the presence of several circular and linear (mega)plasmids, which harbour peculiar catabolic and biosynthetic genes. Within Rhodococcus genus, R. opacus strain PD630 is considered a model oleaginous strain for its ability to produce and accumulate lipids (mostly triacyglycerols, TAGs) using different carbon sources, including low-cost and renewable resources such as lignocellulose. Notably, under specific growth conditions, this strain is capable of accumulating up to 80% of its cellular dry weight in TAGs; that is a rare feature in the prokaryotic and eukaryotic kingdoms. Multi-omic approaches have been applied to obtain system-level information about metabolic and regulatory pathways involved in these biosynthetic processes. Novel molecular tools for genome editing (CRISPR/Cas9 and recombineering) have been recently developed highlighting the possible utilization of R. opacus PD630 as synthetic biology platform for lipids production.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2024

Covalent integration of polymers and porous organic frameworks

Covalent integration of polymers and porous organic frameworks (POFs), including metal-organic frameworks (MOFs), covalent organic frameworks (COFs) and hydrogen-bonded organic frameworks (HOFs), represent a promising strategy for overcoming the existing limitations of traditional porous materials. This integration allows for the combination of the advantages of polymers, i.e., flexibility, processability and chemical versatility etc., and the superiority of POFs, like the structural integrity, tunable porosity and the high surface area, creating a type of hybrid materials. These resulting polymer-POF hybrid materials exhibit enhanced mechanical strength, chemical stability and functional diversity, thus opening up new opportunities for applications across a large variety of fields, such as gas separation, catalysis, biomedical applications, environmental remediation and energy storage. In this review, an overview of synthetic routes and strategies on how to covalently integrate different polymers with various POFs is discussed, especially with a particular focus on methods like polymerization within, on and among POF structures. To investigate the unique properties and functions of these resultant hybrid materials, the characterization techniques, including nuclear magnetic resonance spectroscopy (NMR), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), transmission electron microscopy (TEM) and scanning electron microscopy (SEM), gas adsorption analysis (BET) and computational modeling and machine learning, are also presented. The ability of polymer-POFs to manipulate the pore environments at the molecular level affords these materials a wide range of applications, providing a versatile platform for future advancements in material science. Looking forward, to fully realize the potential of these hybrid materials, the authors highlight the scalability, green synthesis methods, and potential for stimuli-responsive polymer-POF materials as critical areas for future research.

Hossain, Md Amjad↗

DOE OSTI2023

Piezoelectric Energy Harvesting Gyroscopes: Comparative Modeling and Effectiveness

Given its versatility in drawing power from many sources in the natural world, piezoelectric energy harvesting (PEH) has become increasingly popular. However, its energy harvesting capacities could be enhanced further. Here, a mathematical model that accurately simulates the dynamic behavior and energy harvested can facilitate further improvements in the performance of piezoelectric devices. One of the goals of this study is to create a dependable reduced-order model of a multi-purpose gyroscope. This model will make it possible to compute the harvested voltage and electrical power in a semi-analytical manner. The harvested voltage is often modeled as an average value across the whole electrode surface in piezoelectric devices. We propose a model which provides practical insights toward optimizing the performance of the system by considering a spatially varying electric field across the electrode surface length. Our framework allows investigation of the limits of applicability of the modeling assumptions across a range of load resistances. The differential quadrature method (DQM) provides the basis for the suggested numerical solution. The model is also employed to examine energy harvesting under various resistance loads. The newly developed spatially varying model is evaluated for open- and closed-circuit conditions and is proved to be accurate for various values of load resistance that have not previously been considered. The results show that using a spatially varying model is more versatile when modeling the performance of the piezoelectric multifunctional energy harvester. The performance may be accurately captured by the model for load resistances ranging between 103 Ω and 108 Ω. At optimum load resistance and near 65 KHz, the maximum power output predicted by the spatially varying (SV) model is 1.3 mV, 1.5 mV for the open-circuit (OC) model, and 2.1 mV for the closed circuit (CE) model. At a high-load resistance, the SV and OC models all predict the maximum power output to be 1.9 mV while the CE model predicted the maximum voltage to be 3 mV.

42 ENGINEERING↗

DOE OSTI2023

Developing Enzyme Immobilization with Fibrous Membranes: Longevity and Characterization Considerations

Fibrous membranes offer broad opportunities to deploy immobilized enzymes in new reactor and application designs, including multiphase continuous flow-through reactions. Enzyme immobilization is a technology strategy that simplifies the separation of otherwise soluble catalytic proteins from liquid reaction media and imparts stabilization and performance enhancement. Flexible immobilization matrices made from fibers have versatile physical attributes, such as high surface area, light weight, and controllable porosity, which give them membrane-like characteristics, while simultaneously providing good mechanical properties for creating functional filters, sensors, scaffolds, and other interface-active biocatalytic materials. This review examines immobilization strategies for enzymes on fibrous membrane-like polymeric supports involving all three fundamental mechanisms of post-immobilization, incorporation, and coating. Post-immobilization offers an infinite selection of matrix materials, but may encounter loading and durability issues, while incorporation offers longevity but has more limited material options and may present mass transfer obstacles. Coating techniques on fibrous materials at different geometric scales are a growing trend in making membranes that integrate biocatalytic functionality with versatile physical supports. Biocatalytic performance parameters and characterization techniques for immobilized enzymes are described, including several emerging techniques of special relevance for fibrous immobilized enzymes. Diverse application examples from the literature, focusing on fibrous matrices, are summarized, and biocatalyst longevity is emphasized as a critical performance parameter that needs increased attention to advance concepts from lab scale to broader utilization. This consolidation of fabrication, performance measurement, and characterization techniques, with guiding examples highlighted, is intended to inspire future innovations in enzyme immobilization with fibrous membranes and expand their uses in novel reactors and processes.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

Module-Fluidics: Building Blocks for Spatio-Temporal Microenvironment Control

Generating the desired solute concentration signal in micro-environments is vital to many applications ranging from micromixing to analyzing cellular response to a dynamic microenvironment. We propose a new modular design to generate targeted temporally varying concentration signals in microfluidic systems while minimizing perturbations to the flow field. The modularized design, here referred to as module-fluidics, similar in principle to interlocking toy bricks, is constructed from a combination of two building blocks and allows one to achieve versatility and flexibility in dynamically controlling input concentration. The building blocks are an oscillator and an integrator, and their combination enables the creation of controlled and complex concentration signals, with different user-defined time-scales. We show two basic connection patterns, in-series and in-parallel, to test the generation, integration, sampling and superposition of temporally-varying signals. All such signals can be fully characterized by analytic functions, in analogy with electric circuits, and allow one to perform design and optimization before fabrication. Such modularization offers a versatile and promising platform that allows one to create highly customizable time-dependent concentration inputs which can be targeted to the specific application of interest.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2020

Coordination Chemistry in the Structural and Functional Exploration of Actinide-Based Metal-Organic Frameworks

The coordination chemistry between inorganic and organic species can be optimally exemplified by metal–organic frameworks (MOFs), whose structures and functionalities can be rationally designed from these highly tunable building blocks. The high porosity, stability, and versatile functionalities of MOFs have attracted wide-spread attention from energy-related research and pollution remediation to biomedical applications. A unique and underexplored subset of these materials are MOFs based on actinide nodes; these MOFs have distinguished themselves as a unique platform for investigating the versatile oxidation states, reactivity, and coordination chemistry of actinides. Herein, we will focus on the rational design and synthesis of actinide-based MOFs under the general guidelines of coordination chemistry for their structural and functional explorations. The dimensionality, topology, and structures of actinide-based MOFs can be controlled by selecting pre-designed building blocks of actinide-based nodes and organic linkers with certain desired coordination geometries and functionalities. Furthermore, these unique actinide-based MOFs have shown promise for applications in nuclear waste mitigation, pollution control, and catalysis.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2011

Complete genome sequence of Desulfobulbus propionicus type strain (1pr3 T )

Desulfobulbus propionicus Widdel 1981 is the type species of the genus Desulfobulbus, which belongs to the family Desulfobulbaceae. The species is of interest because of its great implication in the sulfur cycle in aquatic sediments, its large substrate spectrum and a broad versatility in using various fermentation pathways. The species was the first example of a pure culture known to disproportionate elemental sulfur to sulfate and sulfide. This is the first completed genome sequence of a member of the genus Desulfobulbus and the third published genome sequence from a member of the family Desulfobulbaceae. The 3,851,869 bp long genome with its 3,351 protein-coding and 57 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2013

Complete genome sequence of Granulicella mallensis type strain MP5ACTX8(T), an acidobacterium from tundra soil

Granulicella mallensis MP5ACTX8(T) is a novel species of the genus Granulicella in subdivision 1 of Acidobacteria. G. mallensis is of ecological interest being a member of the dominant soil bacterial community active at low temperatures and nutrient limiting conditions in Arctic alpine tundra. G. mallensis is a cold-adapted acidophile and a versatile heterotroph that hydrolyzes a suite of sugars and complex polysaccharides. Genome analysis revealed metabolic versatility with genes involved in metabolism and transport of carbohydrates. These include gene modules encoding the carbohydrate-active enzyme (CAZyme) family involved in breakdown, utilization and biosynthesis of diverse structural and storage polysaccharides including plant based carbon polymers. The genome of Granulicella mallensis MP5ACTX8(T) consists of a single replicon of 6,237,577 base pairs (bp) with 4,907 protein-coding genes and 53 RNA

Acidobacteria↗

DOE OSTI2013

Complete genome sequence of Granulicella tundricola type strain MP5ACTX9T, an Acidobacteria from tundra soil

Granulicella tundricola strain MP5ACTX9T is a novel species of the genus Granulicella in subdivision 1 Acidobacteria. G. tundricola is a predominant member of soil bacterial communities, active at low temperatures and nutrient limiting conditions in Arctic alpine tundra. The organism is a cold-adapted acidophile and a versatile heterotroph that hydro-lyzes a suite of sugars and complex polysaccharides. Genome analysis revealed metabolic versatility with genes involved in metabolism and transport of carbohydrates, including gene modules encoding for the carbohydrate-active enzyme (CAZy) families for the break-down, utilization and biosynthesis of diverse structural and storage polysaccharides such as plant based carbon polymers. The genome of G. tundricola strain MP5ACTX9T consists of 4,309,151 bp of a circular chromosome and five mega plasmids with a total genome con-tent of 5,503,984 bp. The genome comprises 4,705 protein-coding genes and 52 RNA genes.

Acidobacteria↗

NASA NTRS1972

Coordination and establishment of centralized facilities and services for the University of Alaska ERTS survey of the Alaskan environment

The author has identified the following significant results. Specifications have been prepared for the engineering design and construction of a digital color display unit which will be used for automatic processing of ERTS data. The color display unit is a disk refresh memory with computer interfaced input and a color cathode ray tube output display. The system features both analog and digital post disk data manipulation and a versatile color coding device suitable for displaying not only images, but also computer generated graphics such as diagrams, maps, and overlays. Input is from IBM compatible 9 track, 800 BPI tapes, as generated by an IBM 360 computer. ERTS digital tapes are read into the 360, where various analyses such as maximum likelihood classification are performed and the results are written on a magnetic tape which is the input to the color display unit. The greatest versatility in the data manipulation area is provided by the minicomputer built into the color display unit, which is off-line from the main 360 computer. The minicomputer is able to read any line from the refresh disk and place it in its 4K-16 bit memory. Considerable flexibility is available for post-processing enhancement of images by the investigator.

Belon, A. E.↗

NASA NTRS1974

The case for a high-speed research airplane - Results from an in-house study

Results of a study aimed at determining the feasibility of developing a single versatile high-speed research airplane (HSRA) which will meet flight research needs of both Mach 3 to 5 JP-fueled military aircraft and of hydrogen-fueled aircraft, both military and civil, for speeds up to Mach 10. The proposed HSRA design concept is an air-launched, rocket-boosted research aircraft designed to accommodate a wide variety of large-scale propulsive and structural flight research experiments. The aircraft is a 60-foot-long discrete wing-body concept with high wings and a center-line vertical tail. Heat sink shields are proposed as an effective and versatile method of achieving low-cost, low-risk thermal protection of the all-aluminum primary structure.-

Kirkham, F. S.↗

NASA NTRS1973

The United States Space Shuttle system, its international availability for science and applications

The many missions under consideration require that the Shuttle, Tug, and Spacelab be extremely versatile. This versatility is being designed into all aspects of the systems. It is believed it will be possible to have simple interfaces between payloads and the basic spacecraft. With capability in the Shuttle to fly into orbits ranging up to polar inclination, and the capability in the Tug to fly to the planets, the combination appears capable of flying all of the missions identified as probable for the 1980s. By taking man into space with his instruments in the Spacelab it is believed that a high degree of mission success can be achieved with less emphasis in instrument perfection than is demanded today.

Culbertson, P. E.↗

NASA NTRS1975

Application of LSI to signal detection: The deltic DFPCC

The development of the DELTIC DFPCC serial mode signal processor is discussed. The processor is designed to detect in the presence of background noise a signal coded into the zero crossings of the waveform. The unique features of the DELTIC DFPCC include versatility in handling a variety of signals and relative simplicity in implementation. A theoretical performance model is presented which predicts the expected value of the output signal as a function of the input signal to noise ratio. Experimental results obtained with the prototype system, which was breadboarded with LSI, MSI and SSI components, are given. The device was compared with other LSI schemes for signal processing and it was concluded that the DELTIC DFPCC is simpler and in some cases more versatile than other systems. With established LSI technology, low frequency systems applicable to sonar and similar problems are feasible.

Gassaway, J. D.↗

NASA NTRS1976

Television cameras employing solid-state imaging sensors for manned spacecraft applications

The Space Shuttle television system incorporates a versatile complement of TV equipment which allows the positioning, quantity, and performance capabilities of the multiple TV cameras to vary as dictated by the particular mission. However, current TV camera technology employing glass enclosed, vacuum processed image sensors results in a bulky device that restricts its location and versatility. Problems and potential solutions that can be achieved through the employment of solid-state image sensors for both monochrome and color applications are discussed.

Embrey, B. C., Jr.↗

NASA NTRS1980

Biomedical technology transfer. Applications of NASA science and technology

Ongoing projects described address: (1) intracranial pressure monitoring; (2) versatile portable speech prosthesis; (3) cardiovascular magnetic measurements; (4) improved EMG biotelemetry for pediatrics; (5) ultrasonic kidney stone disintegration; (6) pediatric roentgen densitometry; (7) X-ray spatial frequency multiplexing; (8) mechanical impedance determination of bone strength; (9) visual-to-tactile mobility aid for the blind; (10) Purkinje image eyetracker and stabilized photocoalqulator; (11) neurological applications of NASA-SRI eyetracker; (12) ICU synthesized speech alarm; (13) NANOPHOR: microelectrophoresis instrument; (14) WRISTCOM: tactile communication system for the deaf-blind; (15) medical applications of NASA liquid-circulating garments; and (16) hip prosthesis with biotelemetry. Potential transfer projects include a person-portable versatile speech prosthesis, a critical care transport sytem, a clinical information system for cardiology, a programmable biofeedback orthosis for scoliosis a pediatric long-bone reconstruction, and spinal immobilization apparatus.

Harrison, D. C.↗

NASA NTRS1982

Imaging spectrometer technologies for advanced Earth remote sensing

A major requirement of multispectral imaging systems for advanced Earth remote sensing is the provision for greater spectral resolution and more versatile spectral band selection. The imaging spectrometer instrument concept provides this versatility by the combination of pushbroom imaging and spectrally dispersing optics using area array detectors in the focal plane. The shuttle imaging spectrometer concept achieves 10- and 20-meter ground instantaneous fields of view with 20-nanometer spectral resolution from Earth Orbit. Onboard processing allows the selection of spectral bands during flight; this, in turn, permits the sensor parameters to be tailored to the experiment objectives. Advances in optical design, infrared detector arrays, and focal plane cooling indicate the feasibility of the instrument concept and support the practicability of a validation flight experiment for the shuttle in the late 1980s.

Wellman, J. B.↗

NASA NTRS1983

MINIVER upgrade for the AVID system. Volume 1: LANMIN user's manual

The successful design of thermal protection systems for vehicles operating in atmosphere and near space environments requires accurate analyses of heating rate and temperature histories encountered along a trajectory. For preliminary design calculations, however, the requirement for accuracy must be tempered by the need for speed and versatility in computational tools used to determine thermal environments and structural thermal response. The MINIVER program has been found to provide the proper balance between versatility, speed and accuracy for an aerothermal prediction tool. The advancement in computer aided design concepts at Langley Research Center (LaRC) in the past few years has made it desirable to incorporate the MINIVER program into the LaRC Advanced Vehicle Integrated Design, AVID, system. In order to effectively incorporate MINIVER into the AVID system, several changes to MINIVER were made. The thermal conduction options in MINIVER were removed and a new Explicit Interactive Thermal Structures (EXITS) code was developed. Many upgrades to the MINIVER code were made and a new Langley version of MINIVER called LANMIN was created. The theoretical methods and subroutine functions used in LANMIN are described.

Engel, C. D.↗

NASA NTRS1983

MINIVER upgrade for the AVID system. Volume 3: EXITS user's and input guide

The successful design of thermal protection systems for vehicles operating in atmosphere and near-space environments requires accurate analyses of heating rate and temperature histories encountered along a trajectory. For preliminary design calculations, however, the requirement for accuracy must be tempered by the need for speed and versatility in computational tools used to determine thermal environments and structural thermal response. The MINIVER program was found to provide the proper balance between versatility, speed and accuracy for an aerothermal prediction tool. The advancement in computer aided design concepts at Langley Research Center (LaRC) in the past few years has made it desirable to incorporate the MINIVER program into the LaRC Advanced Vehicle Integrated Design, AVID, system. In order to effectively incorporate MINIVER into the AVID system, several changes to MINIVER were made. The thermal conduction options in MINIVER were removed and a new Explicit Interactive Thermal Structures (EXITS) code was developed. Many upgrades to the MINIVER code were made and a new Langley version of MINIVER called LANMIN was created.

Pond, J. E.↗