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At least 937 records · Page 52

Intramolecular activation of a Ca(2+)-dependent protein kinase is disrupted by insertions in the tether that connects the calmodulin-like domain to the kinase

Ca(2+)-dependent protein kinases (CDPK) have a calmodulin-like domain (CaM-LD) tethered to the C-terminal end of the kinase. Activation is proposed to involve intramolecular binding of the CaM-LD to a junction sequence that connects the CaM-LD to the kinase domain. Consistent with this model, a truncated CDPK (DeltaNC) in which the CaM-LD has been deleted can be activated in a bimolecular interaction with an isolated CaM-LD or calmodulin, similar to the activation of a calmodulin-dependent protein kinase (CaMK) by calmodulin. Here we provide genetic evidence that this bimolecular activation requires a nine-residue binding segment from F436 to I444 (numbers correspond to CPK-1 accession number L14771). Two mutations at either end of this core segment (F436/A and VI444/AA) severely disrupted bimolecular activation, whereas flanking mutations had only minor effects. Intramolecular activation of a full-length kinase was also disrupted by a VI444/AA mutation, but surprisingly not by a F436/A mutation (at the N-terminal end of the binding site). Interestingly, intramolecular but not bimolecular activation was disrupted by insertion mutations placed immediately downstream of I444. To show that mutant enzymes were not misfolded, latent kinase activity was stimulated through binding of an antijunction antibody. Results here support a model of intramolecular activation in which the tether (A445 to G455) that connects the CaM-LD to the kinase provides an important structural constraint and is not just a simple flexible connection.

NASA Discipline Plant Biology↗

Space Mirror Alignment System

An optical alignment mirror mechanism (AMM) has been developed with angular positioning accuracy of +/-0.2 arcsec. This requires the mirror s linear positioning actuators to have positioning resolutions of +/-112 nm to enable the mirror to meet the angular tip/tilt accuracy requirement. Demonstrated capabilities are 0.1 arc-sec angular mirror positioning accuracy, which translates into linear positioning resolutions at the actuator of 50 nm. The mechanism consists of a structure with sets of cross-directional flexures that enable the mirror s tip and tilt motion, a mirror with its kinematic mount, and two linear actuators. An actuator comprises a brushless DC motor, a linear ball screw, and a piezoelectric brake that holds the mirror s position while the unit is unpowered. An interferometric linear position sensor senses the actuator s position. The AMMs were developed for an Astrometric Beam Combiner (ABC) optical bench, which is part of an interferometer development. Custom electronics were also developed to accommodate the presence of multiple AMMs within the ABC and provide a compact, all-in-one solution to power and control the AMMs.

Jau, Bruno M.↗

On the structure of the Io Torus

Since the plasma flow near Io is reduced, neutrals originating in charge exchange are not energetic enough to leave the Jovian system and are therefore distributed over an extensive region, as indicated by the sodium cloud. New ions subsequently created in the distributed neutral atomic cloud as a result of charge exchange or electron impact ionization are picked up by the corotating magnetic field, and the radial current driven by the pickup process cannot close in the Io torus, so that it must instead be connected to the planetary ionosphere by field-aligned currents. These field-aligned currents will flow away from the equator at the outer edge of the neutral cloud, and towards it at the inner edge. It is found that the Jovian ionospheric photoelectrons also cannot supply the current flowing away from the equator, so that torus ions accelerated by a parallel electric field could be involved. The parallel potential drop is large enough to push the torus into the Jovian atmosphere, explaining both the sharp, discontinuous change of flux tube content and ion temperature at L equals 5.6, as well as the generation of Auroral-type hiss at that point.

Goertz, C. K.↗

Identification of geostructures of continental crust particularly as they relate to mineral resource evaluation

The author has identified the following significant results. Lakes in the Arctic Coastal Plain of Alaska are dominantly elongate, with their long axes parallel and trending about N 9 W. On ERTS-1 imagery, an additional strong east-trending regional lineation, not previously recognized on aerial photographs or in field study, was noted. In addition, the alignment of many small lakes forms a large and a small ellipse superimposed on the regional lineation. Fischer and Lathram find that the trend of this lineation is parallel to the trend of deflections in contours of the magnetic and gravity fields in the area, and parallel to westerly deflections in the northwest ends of northwest-trending folds mapped to the south. These data suggest that heretofore unsuspected structures may be concealed beneath Quaternary mantling sediments in the area of the image. The strata in these folds would be younger than those tapped by the oil wells of the Umiat field to the south, and favorable sandstone facies may occur in the area. The significance of these observations to oil exploration in northern Alaska needs to be evaluated further.

Gryc, G.↗

Space station architecture, module, berthing hub, shell assembly, berthing mechanism and utility connection channel

The geometric form of a space station is presented that includes a description of a plurality of modules and berthing hubs, joined by interconnections which are sideways connectable. The modules and hubs are fastened together in a triangular configuration in three dimensions. The interconnections include a pair of opposed, axially aligned, flanged ports and a clamp latch formed from a plurality of sections hinged along their length and extending circumferentially around the flanged ports. A hermetic seal is formed between the ports. A utilities connection channel extends between the ports. The channel has a shell with utilities connectors movable between an extended position to mating connectors in the modules and a withdrawn position. Assembly sequence and common module shell structure is detailed.

Cohen, M. M.↗

The Generation of Smooth High Speed Solar Wind from Plume-Interplume Mixing

Plumes and rays are magnetic field aligned density striations in coronal holes with different values of plasma beta. The overall plasma beta is very small in the low corona but exceeds unity beyond 15-20 solar radius. High speed solar wind reported beyond 0.3 AU is relatively smooth and uniform and known to originate from the much filamented coronal hole. Thus the obvious question is how to generate a smooth solar wind from seemingly filamentary structure. Hence one has to find a mechanism to substantiate this apparent observed (Ulysses) phenomenon. To do this we model plumes as jets (or wakes) of plasma emitted from the solar surface. The shear between a jet and its ambient is known to become unstable to the MHD Kelvin-Helmholtz ("KH") instability if the Alfven Mach number of the jet is greater than one and the uniform external magnetic field is small. Starting with a simple configuration we consider a jet of half thickness R, having uniform density and uniform internal magnetic field. The external medium has also a uniform density and uniform magnetic field. The jet is perturbed at the boundary with a linear amplitude and fixed frequency. We simulate the coronal jet using the 3D ZEUS code. The first results indicate the slab jet is unstable to the MHD KH instability at 5-10 solar radius for some angle of wave propagation. The propagating instability may smooth the filamented flow. It may also produce the entrained Alfvenic fluctuations observed by Ulysses in the high speed wind. We are at present determining the parameters which induce large growth rate. This may clarify the mystery behind the emergence of fast smooth solar wind from very filamentary structures in coronal holes. Also, using the dispersion relation already available for such a flow we obtain some general description of the instability criteria for the KH instability at a jet interface.

Parhi, Shyamsundar↗

A Candle in the Wind: A Radio Filament in the Core of the A3562 Galaxy Cluster

Using a MeerKAT observation of the galaxy cluster A3562 (a member of the Shapley Supercluster), we have discovered a narrow, long and straight, very faint radio filament, which branches out at a straight angle from the tail of a radio galaxy located in projection near the core of the cluster. The radio filament spans 200 kpc and aligns with a sloshing cold front seen in the X-rays, staying inside the front in projection. The radio spectral index along the filament appears uniform (within large uncertainties) atα' −1.5. We propose that the radio galaxy is located outside the cold front, but dips its tail under the front. The tangential wind that blows there may stretch the radio plasma from the radio galaxy into a filamentary structure. Some reacceleration is needed in this scenario to maintain the radio spectrum uniform. Alternatively, the cosmic ray electrons from that spot in the tail can spread along the cluster magnetic field lines, straightened by that same tangential flow, via anomalously fast diffusion. Our radio filament can provide constraints on this process. We also uncover a compact radio source at the Brightest Cluster Galaxy (BCG) that is 2–3 orders of magnitude

S Giacintucci↗

The distortion of the magnetotail equilibrium structure by a net cross-tail magnetic field

Explicit solutions are presented for quiet, quasi-static magnetotail configurations in the presence of a net cross-tail magnetic field component in addition to field components associated with tail flaring. The solutions are derived using the general three-dimensional equilibrium theory of Birn (1987). It is found that the net By(N) field varies only weakly along field lines and across the tail. The presence of the net By(N) in the neutral sheet implies that a part of the cross-tail current becomes field-aligned, flowing from the southern to the northern hemisphere for By(N) larger than zero and from north to south for By(n) less than zero. The deformations of magnetic flux surfaces suggest that there may be a class of field lines extending from the earth into the magnetosheath region through the low-latitude flanks of the tail. A topological connection is found of the low-latitude region near the magnetopause with the plasma sheet boundary layer, separating closed plasma sheet and open lobe field lines.

Birn, J.↗

C/NOFS Observations of Electromagnetic Coupling Between Magnetically Conjugate MSTID Structures

This report demonstrates empirically that couplings between magnetically conjugate medium-scale traveling ionospheric disturbances (MSTIDs) are electromagnetic in nature. This is accomplished by comparing plasma density, electric, and magnetic perturbations sampled simultaneously by sensors on the Communication Navigation Outage Forecasting System (CNOFS) satellite. During the period of interest on 17 February 2010, CNOFS made three consecutive orbits while magnetically conjugate to the field of view of an all-sky imager located at El Leoncito, Argentina (31.8degS, 69.3degW). Imaged 630.0 nm airglow was characterized by alternating bands of relatively bright and dark emissions that were aligned from northeast to southwest and propagated toward the northwest, characteristic of MSTIDs in the southern hemisphere. Measurable Poynting fluxes flow along the Earths magnetic field (S) from generator to load hemispheres. While S was predominantly away from the ionosphere above El Leoncito, interhemispheric energy flows were not one-way streets. Measured Poynting flux intensities diminished with time over the three CNOFS passes, suggesting that source mechanisms of MSTIDs were absent or that initial impedance mismatches between the two hemispheres approached an equilibrium status.

Burke, W. J.↗

Star of Condor - A strontium critical velocity experiment, Peru, 1983

'Star of Condor' was a critical velocity experiment using Sr vapor produced in a radial shaped charge, which was carried to 571.11 km altitude on a Taurus-Tomahawk rocket launched from Punto Lobos, Peru, and detonated in the plane of the magnetic field lines so that all ranges of pitch angles from parallel to B to perpendicular to B were covered. Sr has a critical velocity of 3.3 km/s, and from observation, 42.5 percent of the neutral Sr gas had a velocity component perpendicular to B exceeding that value. No Sr ion emissions were detected shortly after the burst with usual TV integration times. However, about 10 min after the detonation a faint field-aligned streak was discovered with long TV integration times. The brightness is estimated as 5 R, which, combined with the streak geometry, implies an ion production of 2.4 x 10 to the 19th ions. This is only 0.0036 percent ionization of the Sr vapor. All the ions could easily have been produced by thermal ionization from the original detonation thermal distribution. The breakup of the Sr gas into small bloblike structures may have allowed the high-energy electrons to escape before an ionization cascade could be produced. For whatever reason, the Alfven mechanism proposed for space plasmas in the absence of laboratory walls did not produce an ionization cascade in the experiment.

Wescott, E. M.↗

A general association between discrete auroras and ion precipitation from the tail

Observations from the spinning polar-orbiting S3-3 satellite were used to compare the locations of discrete auroral arcs (defined to be regions containing particle distributions consistent with field-aligned potential drops of not less than 0.5 kV) with regions of isotropic ion precipitation. It was found that the regions of discrete aurora are almost exclusively confined to the region of isotropic ion precipitation at all local times studied (polar cap arcs and local times near noon were not considered). It was also found that, throughout the local time interval studied, the discrete aurora was generally associated with spatial structure and boundaries in the precipitating ions, indicating that arc generation may be associated with structure in the particle population within the tail current sheet.

Lyons, L. R.↗

Towards a Realistic Pulsar Magnetosphere

We present the magnetic and electric field structures as well as the currents ami charge densities of pulsar magnetospberes which do not obey the ideal condition, E(raised dot) B = O. Since the acceleration of particles and the production of radiation requires the presence of an electric field component parallel to the magnetic field, E(sub ll) the structure of non-Ideal pulsar magnetospheres is intimately related to the production of pulsar radiation. Therefore, knowledge of the structure of non-Ideal pulsar maglletospheres is important because their comparison (including models for t he production of radiation) with observations will delineate the physics and the parameters underlying the pulsar radiation problem. We implement a variety of prescriptions that support nonzero values for E(sub ll) and explore their effects on the structure of the resulting magnetospheres. We produce families of solutions that span the entire range between the vacuum and the (ideal) Force-Free Electrodynamic solutions. We also compute the amount of dissipation as a fraction of the Poynting flux for pulsars of different angles between the rotation and magnetic axes and conclude that tltis is at most 20-40% (depending on t he non-ideal prescription) in the aligned rotator and 10% in the perpendicular one. We present also the limiting solutions with the property J = pc and discuss their possible implicatioll on the determination of the "on/ off" states of the intermittent pulsars. Finally, we find that solutions with values of J greater than those needed to null E(sub ll) locally produce oscillations, potentially observable in the data.

Kalapotharakos, Constantinos↗

Full Scale Flat Floor Testing of a 500-M2-Class Solar Sail Deployer

Solar sailing missions rely on deployable systemsfor large area to mass ratios once in space, whilestill being small enough for launcher envelopes inthe stowed configuration. Many of thesedeployable systems feature booms that areflattened and subsequently coiled onto aspool/hub. As part of a collaborative deployablespace structures research effort of NASA and DLR,a boom deployment mechanism for a future 500 m²solar sail has been developed since 2017. To achieve the respective solar sail size goal, 16.5m long booms produced by NASA were integratedinto a DLR-designed deployer mechanism. Thisconsiderable size, as well as the lightweightconstruction of the booms and respectivedeployable systems makes ground testing asignificant challenge. Some systems for gravitycompensation and boom alignment will bepresented in the paper. However, the main focus isthe functional flat floor testing of the integratedboom-deployment mechanism system, as well asits challenges. The testing performed includes full deployment aswell as stowage of the booms. Both have beenperformed multiple times. The latter is one of thekey parameters determining packaging efficiency,which in turn confirms design assumptions. Duringsystem development, small scale tests and modelshave been used in preparation of flat floor testingof the 33 m span cross of the full-scale booms anddeployment mechanism. Hence a small chapter isalso devoted to analysing the differences inbehaviour between small- and full-scaledeployment systems. More parameters that are vital to design decisionshave been determined this way, such as drivingbelt force or hub brake torque. This paper alsofocuses on development goals and needs forfuture steps to achieve higher levels of technologyreadiness, such as the balancing of driving motorforce, synchronisation of its transmission and thecountering hub brake torque

Deployment mechanism↗

Structural relations between collagen and mineral in bone as determined by high voltage electron microscopic tomography

Aspects of the ultrastructural interaction between collagen and mineral crystals in embryonic chick bone have been examined by the novel technique of high voltage electron microscopic tomography to obtain three-dimensional information concerning extracellular calcification in this tissue. Newly mineralizing osteoid along periosteal surfaces of mid-diaphyseal regions from normal chick tibiae was embedded, cut into 0.25 microns thick sections, and documented at 1.0 MV in the Albany AEI-EM7 high voltage electron microscope. The areas of the tissue studied contained electron dense mineral crystals associated with collagen fibrils, some marked by crystals disposed along their cylindrically shaped lengths. Tomographic reconstructions of one site with two mineralizing fibrils were computed from a 5 degrees tilt series of micrographs over a +/- 60 degrees range. Reconstructions showed that the mineral crystals were platelets of irregular shape. Their sizes were variable, measured here up to 80 x 30 x 8 nm in length, width, and thickness, respectively. The longest crystal dimension, corresponding to the c-axis crystallographically, was generally parallel to the collagen fibril long axis. Individual crystals were oriented parallel to one another in each fibril examined. They were also parallel in the neighboring but apparently spatially separate fibrils. Crystals were periodically (approximately 67 nm repeat distance) arranged along the fibrils and their location appeared to correspond to collagen hole and overlap zones defined by geometrical imaging techniques. The crystals appeared to be continuously distributed along a fibril, their size and number increasing in a tapered fashion from a relatively narrow tip containing smaller and infrequent crystals to wider regions having more densely packed and larger crystals. Defined for the first time by direct visual 3D imaging, these data describe the size, shape, location, orientation, and development of early crystals in normal bone collagen. The results suggest that platelet-shaped crystals are arranged in channels or grooves which are formed by collagen hole zones in register and that crystal sizes may exceed the dimensions of hole zones. Such data agree with those from mineral-matrix interaction in normally calcifying avian tendon obtained by similar high voltage tomographic means, but in addition they indicate a possible gradual and continuous deposition of crystals in collagen of bone unlike tendon and imply that individual collagen fibrils in local regions of osteoid are organized such that they all may be aligned in a coherent manner.

NASA Discipline Number 40-40↗

Influence of pressure driven secondary flows on the behavior of turbofan forced mixers

A finite difference procedure was developed to analyze the three dimensional subsonic turbulent flows in turbofan forced mixer nozzles. The method is based on a decomposition of the velocity field into primary and secondary flow components which are determined by solution of the equations governing primary momentum, secondary vorticity, thermal energy, and continuity. Experimentally, a strong secondary flow pattern was identified which is associated with the radial inflow and outflow characteristics of the core and fan streams and forms a very strong vortex system aligned with the radial interface between the core and fan regions. A procedure was developed to generate a similar generic secondary flow pattern in terms of two constants representing the average radial outflow or inflow in the core and fan streams as a percentage of the local streamwise velocity. This description of the initial secondary flow gave excellent agreement with experimental data. By identifying the nature of large scale secondary flow structure and associating it with characteristic mixer nozzle behavior, it is felt that the cause and effect relationship between lobe design and nozzle performance can be understood.

Anderson, B.↗

ULF waves in the low-latitude boundary layer and their relationship to magnetospheric pulsations - A multisatellite observation

Combined data from five spacecraft have been analyzed to understand the structure of the low-latitude boundary layer (LLBL), the ULF waves in the LLBL, and the relation of the LLBL waves to magnetic pulsations in the magnetosphere. Both the intensity and the pitch angle distribution of the particles clearly show the presence of the LLBL for the time interval studied. The average magnetic field is rotated slightly at or near the LLBL/magnetosphere interface, which is consistent with a field-aligned current sheet with region 1 flow direction. For ULF waves, a 5-10 min compressional perturbation is present both in the LLBL and the magnetosphere. In the boundary layer, large-amplitude transverse oscillations are present. Magnetic Pc 4-5 pulsations are present in the magnetosphere with azimuthal perturbations and position-dependent frequency.

Takahashi, Kazue↗

Synthesis and Characterization of a Chitosan Derivative for Electro-Optical Applications

Chitin is a naturally occurring polymer of alpha(1-4) poly N-acetylglucosamine found primarily in the shells of crustaceans and insects. This polymer is chemically and thermally stable and physically durable as a consequence of hydrogen bonding which causes the alignment and ordering of the polymer chains into microcrystals which aggregate into sheets with chiral nematic order. Industry has attempted to take advantage of chitin's properties and low cost (chitin is a waste product of the shellfish industry) to produce durable fibers and other products. This has been largely unsuccessful because of chitin's non reactivity and insolubility. Chitosan is the deacetylation product of chitin and retains many of the structural properties of chitin. Unlike chitin, chitosan is soluble in aqueous solution at reduced pH making it easier to be processed into fibers and films than chitin. Chitosan and its derivatives are now used in such commercial applications as wound dressings, waste water treatment, and in pharmaceuticals. In this study, we have synthesized a chitosan derivative, N-para-nitrophenyl chitosan (NPNPC), as a model material with potential applications in electro optics.

Prastofer, Thomas↗

Scattering and the Point Spread Function of the New Generation Space Telescope

Preliminary design work on the New Generation Space Telescope (NGST) is currently under way. This telescope is envisioned as a lightweight, deployable Cassegrain reflector with an aperture of 8 meters, and an effective focal length of 80 meters. It is to be folded into a small-diameter package for launch by an Atlas booster, and unfolded in orbit. The primary is to consist of an octagon with a hole at the center, and with eight segments arranged in a flower petal configuration about the octagon. The comers of the petal-shaped segments are to be trimmed so that the package will fit atop the Atlas booster. This mirror, along with its secondary will focus the light from a point source into an image which is spread from a point by diffraction effects, figure errors, and scattering of light from the surface. The distribution of light in the image of a point source is called a point spread function (PSF). The obstruction of the incident light by the secondary mirror and its support structure, the trimmed corners of the petals, and the grooves between the segments all cause the diffraction pattern characterizing an ideal point spread function to be changed, with the trimmed comers causing the rings of the Airy pattern to become broken up, and the linear grooves causing diffraction spikes running radially away from the central spot, or Airy disk. Any figure errors the mirror segments may have, or any errors in aligning the petals with the central octagon will also spread the light out from the ideal point spread function. A point spread function for a mirror the size of the NGST and having an incident wavelength of 900 nm is considered. Most of the light is confined in a circle with a diameter of 0.05 arc seconds. The ring pattern ranges in intensity from 10(exp -2) near the center to 10(exp -6) near the edge of the plotted field, and can be clearly discerned in a log plot of the intensity. The total fraction of the light scattered from this point spread function is called the total integrated scattering (TIS), and the fraction remaining is called the Strehl ratio. The angular distribution of the scattered light is called the angle resolved scattering (ARS), and it shows a strong spike centered on a scattering angle of zero, and a broad , less intense distribution at larger angles. It is this scattered light, and its effect on the point spread function which is the focus of this study.

Schreur, Julian J.↗