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NASA NTRS2008

Rapid Biochemical Analysis on the International Space Station (ISS): Preparing for Human Exploration of the Moon and Mars

The Lab-on-a-Chip Application Development - Portable Test System, known as LOCAD-PTS, was launched to the International Space Station (ISS) aboard Space Shuttle Discovery (STS-116) on December 9th,2006. Since that time, it has remained onboard ISS and has been operated by the crew on 10 separate occasions LOCAD-PTS is a handheld device for rapid biochemical analysis; it consists of a spectrophotometer, a series of interchangeable cartridges, a pipette and several clean/sterilized swabbing kits to obtain samples from ISS surfaces. Sampling, quantitative analysis and data retrieval is performed onboard, therefore reducing the need to return samples to Earth. Less than 20 minutes are required from sampling to data, significantly faster than existing culture-based methods on ISS, which require 3-5 days. Different cartridges are available for the detection of different target molecules (simply by changing the formulation within each cartridge), thereby maximizing the benefit and applications addressed by a single instrument. Initial tests on ISS have focused on the detection of the bact.erial macromolecule endotoxin, a component of bacterial cell walls. LOCAD-PTS detects endotoxin with a cartridge that contains a formulation known as Limulus Amebocyte Lysate (LAL) assay. LAL is derived from blood of the horseshoe crab, Limulus polyphemus, and detects enodotoxin with an enzyme cascade that triggers generation Of a yellow colored dye, p-nitroanaline. The more p-nitroanaline product, the more endotoxin is in the original sample. To enable quantitative analysis, the absorbance of this color is measured by LOCAD-PTS through a 395 nm filter and compared with an internal calibration curve, to provide a reading on the LED display that ranges from 0.05 Endotoxin Units (EU)/ml to 5 EU/ml. Several surface sites were analyzed within ISS between March 2007 and February 2008, including multiple locations in the US Laboratory Destiny, Node 1 Unity, AMock, and Service Module Zvezda. The goals of this initial study were to i) test the cleanliness of reagents/supplies on orbit, ii) test the crew's ability to collect and process a sample in microgravity without contamination, iii) demonstrate nominal function of the LOCAD-PTS, and iv) provide a general survey of endotoxin within the ISS. The surface sites varied greatly in terms of their frequency-of-use and material texture/composition; from relatively smooth aluminum, to fabric, to the room temperature vulcanizing (RTV) rubber of a Extravehicular Mobility Unit (EMU) spacesuit. Results showed that: i) the swabbing kits and reagents remained clean on orbit, ii) the crew could collect and process a sample without contamination, and iii) the LOCAD-PTS functioned nominally in > 99% of the 55 tests completed. We will present detailed results of the survey of endotoxin on ISS surfaces. These results and technology are important in the near-term - by providing an extra tool in the toolbox for ISS microbial monitoring. They are also important in the longer term as valuable preparation for human exploration of the Moon and Mars. One of the proposed science goals for the human exploration of Mars will be to detect and characterize any indigenous biological molecules that may exist on the Martian surface. To achieve that goal, the crew must have the technology available onboard to differentiate indigenous biology from any terrestrial biological material brought to Mars by the spacecraft and crew (termed 'forward contamination'). The LAL assay is already one of the official methods used by NASA's planetary protection program to certify cleanliness of interplanetary robotic spacecraft prior to launch; and therefore endotoxin is a good marker of forward contamination (as well as other microbial molecules detectable with LOCAD-PTS e.g. box-1, 3-glucan and lipoteichoic acid). Furthermore, the distribution and abundance of these molecules on the ISS provides a good indicator of what to expect on the Crew Exploratioehicle Orion, the lunar lander Antares, and future crewed spacecraft destined for Mars. In addition, technology such as LOCAD-PTS has been proposed to help evaluate forward contamination during lunar surface operations by the crew, as preparation for the human exploration of Mars.

Maule, J.↗

NASA NTRS2006

Development of a Countermeasure to Mitigate Postflight Locomotor Dysfunction

Astronauts returning from space flight experience locomotor dysfunction following their return to Earth. Our laboratory is currently developing a gait adaptability training program that is designed to facilitate recovery of locomotor function following a return to a gravitational environment. The training program exploits the ability of the sensorimotor system to generalize from exposure to multiple adaptive challenges during training so that the gait control system essentially learns to learn and therefore can reorganize more rapidly when faced with a novel adaptive challenge. Evidence for the potential efficacy of an adaptive generalization gait training program can be obtained from numerous studies in the motor learning literature which have demonstrated that systematically varying the conditions of training enhances the ability of the performer to learn and retain a novel motor task. These variable practice training approaches have been used in applied contexts to improve motor skills required in a number of different sports. The central nervous system (CNS) can produce voluntary movement in an almost infinite number of ways. For example, locomotion can be achieved with many different combinations of joint angles, muscle activation patterns and forces. The CNS can exploit these degrees of freedom to enhance motor response adaptability during periods of adaptive flux like that encountered during a change in gravitational environment. Ultimately, the functional goal of an adaptive generalization countermeasure is not necessarily to immediately return movement patterns back to normal. Rather the training regimen should facilitate the reorganization of available sensory and motor subsystems to achieve safe and effective locomotion as soon as possible after long duration space flight. Indeed, this approach has been proposed as a basic feature underlying effective neurological rehabilitation. We have previously confirmed that subjects participating in an adaptive generalization training program using a variety of visuomotor distortions and throwing as the dependent measure can learn to enhance their ability to adapt to a novel sensorimotor environment (Roller et al., 2001). Importantly, this increased adaptability was retained even one month after completion of the training period. Adaptive generalization has been observed in a variety of other tasks requiring sensorimotor transformations including manual control tasks and reaching (Bock et al., 2001, Seidler, 2003) and obstacle avoidance during walking (Lam and Dietz, 2004). Taken together, the evidence suggests that a training regimen exposing crewmembers to variation in locomotor conditions, with repeated transitions among states, may enhance their ability to learn how to reassemble appropriate locomotor patterns upon return from microgravity. We believe exposure to this type of training will extend crewmembers locomotor behavioral repertoires, facilitating the return of functional mobility after long duration space flight. In other words, our proposed training protocol will compel subjects to develop new behavioral solutions under varying sensorimotor demands. Over time subjects will learn to create appropriate locomotor solution more rapidly enabling acquisition of mobility sooner after long-duration space flight. A gait adaptability training program can be superimposed on nominal treadmill exercise activities thus ensuring that no additional crew time is required to perform this type of training regimen and that it can be implemented with current in-flight exercise systems available on the International Space Station.

Bloomberg, J. J.↗

NASA NTRS2002

Interannual Variability of Martian Global Dust Storms: Simulations with a Low-Order Model of the General Circulation

We present simulations of the interannual variability of martian global dust storms (GDSs) with a simplified low-order model (LOM) of the general circulation. The simplified model allows one to conduct computationally fast long-term simulations of the martian climate system. The LOM is constructed by Galerkin projection of a 2D (zonally averaged) general circulation model (GCM) onto a truncated set of basis functions. The resulting LOM consists of 12 coupled nonlinear ordinary differential equations describing atmospheric dynamics and dust transport within the Hadley cell. The forcing of the model is described by simplified physics based on Newtonian cooling and Rayleigh friction. The atmosphere and surface are coupled: atmospheric heating depends on the dustiness of the atmosphere, and the surface dust source depends on the strength of the atmospheric winds. Parameters of the model are tuned to fit the output of the NASA AMES GCM and the fit is generally very good. Interannual variability of GDSs is possible in the IBM, but only when stochastic forcing is added to the model. The stochastic forcing could be provided by transient weather systems or some surface process such as redistribution of the sand particles in storm generating zones on the surface. The results are sensitive to the value of the saltation threshold, which hints at a possible feedback between saltation threshold and dust storm activity. According to this hypothesis, erodable material builds up its a result of a local process, whose effect is to lower the saltation threshold until a GDS occurs. The saltation threshold adjusts its value so that dust storms are barely able to occur.

Pankine, A. A.↗

NASA NTRS2009

Sequential Events Control System (SECS) Overview

This slide presentation will cover the Sequential Events Control System (SECS), which is the Apollo spacecraft subsystem that controls the automatically sequenced functions during the mission and during any a borts that could be performed. Included in this presentation are its general architecture, its integration into and use of the spacecraft' s other systems, and details on the functions it is responsible for c ontrolling during the mission. The objectives are to describe the system's architecture, the major components in the system, and the major system functions.

Interbartolo, Michael↗

NASA NTRS1986

Applications and requirements for real-time simulators in ground-test facilities

This report relates simulator functions and capabilities to the operation of ground test facilities, in general. The potential benefits of having a simulator are described to aid in the selection of desired applications for a specific facility. Configuration options for integrating a simulator into the facility control system are discussed, and a logical approach to configuration selection based on desired applications is presented. The functional and data path requirements to support selected applications and configurations are defined. Finally, practical considerations for implementation (i.e., available hardware and costs) are discussed.

Arpasi, Dale J.↗

NASA NTRS1972

Skylab and its solar astronomy experiments.

The functional characteristics of the Skylab are considered together with general information concerning the spacecraft and its equipment. The Skylab solar instruments are examined, giving attention to two H-alpha telescopes, a white light coronograph, X-ray spectrographic telescopes, a UV scanning polychromator/spectroheliometer, a dual X-ray telescope, an XUV spectrograph/spectroheliograph, and instruments of X-ray/UV solar photography. The Skylab solar observing programs are discussed along with aspects of astronaut training and details regarding the control and display devices of the spacecraft.

Schneider, W. C.↗

NASA NTRS1974

Correlation effects in the theory of combined Doppler and pressure broadening. I - Classical theory

An investigation is conducted of the combined effects of radiator-perturber collisions and radiator translational motion in the context of foreign gas broadening of optical transitions in neutral radiators. Questions concerning the speed-dependent collision frequency are considered and aspects of general theory are explored, taking into account the correlation function, the ensemble average, and the kinetic equation formalism. An elementary solution is discussed along with a one-perturber approximation, inverse power law model calculations, and a comparison with the Voigt profile.

Ward, J.↗

NASA NTRS1974

Heating of the high-latitude thermosphere during magnetically quiet periods

A persistent mid- to high-latitude heating phenomenon is observed in both hemispheres in data from the Ogo 6 quadrupole mass spectrometer. The phenomenon is evidenced by an increase in N2 density (indicative of a thermospheric temperature rise) and a depletion in helium (indicating an upwelling of air). The composition changes maximize near 0900 and 2100 UT, appear to corotate with the local magnetic pole, and are larger near equinox than near the summer solstice. The variation in latitude of the peak in the winter helium density (as a function of UT) is a specific manifestation of this general heating phenomenon.

Reber, C. A.↗

NASA NTRS1973

Measurement of driver/vehicle multiloop response properties with a single disturbance input

Multiloop response properties of controllers are, in general, very difficult to obtain because an independent forcing function is needed for each describing function to be measured, and interpolation procedures may be required to obtain intermediate describing functions at common frequencies. Two examples are provided for the measurement of driver/vehicle multiloop response properties using a single disturbance input. The validity of the procedure is based on current multiloop operator adjustment rules and is made plausible by comparison with experimental data.

Mcruer, D. T.↗

NASA NTRS1978

A mean spherical model for soft potentials: The hard core revealed as a perturbation

The mean spherical approximation for fluids is extended to treat the case of dense systems interacting via soft-potentials. The extension takes the form of a generalized statement concerning the behavior of the direct correlation function c(r) and radial distribution g(r). From a detailed analysis that views the hard core portion of a potential as a perturbation on the whole, a specific model is proposed which possesses analytic solutions for both Coulomb and Yukawa potentials, in addition to certain other remarkable properties. A variational principle for the model leads to a relatively simple method for obtaining numerical solutions.

Rosenfeld, Y.↗

NASA NTRS1977

The Mg II features near 2800A and spectral classification

Relatively high-resolution (about 0.4 A) observations of the Mg II resonance lines at 2795.523 and 2802.698 A as well as their respective subordinate lines at 2797.989 and 2790.768 A are discussed for main-sequence stars, giants, and supergiants. The general behavior of the Mg II lines as a function of spectral type is described. An expression is obtained for the relationship between absolute magnitude and Mg II emission widths. The variation of the absorption widths of the Mg II features with spectral type and luminosity class is briefly summarized.

Kondo, Y.↗

NASA NTRS1979

Feasibility study for a numerical aerodynamic simulation facility. Volume 2: Hardware specifications/descriptions

An FMP (Flow Model Processor) was designed for use in the Numerical Aerodynamic Simulation Facility (NASF). The NASF was developed to simulate fluid flow over three-dimensional bodies in wind tunnel environments and in free space. The facility is applicable to studying aerodynamic and aircraft body designs. The following general topics are discussed in this volume: (1) FMP functional computer specifications; (2) FMP instruction specification; (3) standard product system components; (4) loosely coupled network (LCN) specifications/description; and (5) three appendices: performance of trunk allocation contention elimination (trace) method, LCN channel protocol and proposed LCN unified second level protocol.

Green, F. M.↗

NASA NTRS1979

An out-of-plane CO survey of the first galactic quadrant

An out-of-plane survey of 2.6-mm CO emission from the first quadrant of the galactic disk carried out at Columbia University with a 1.2-meter telescope is reported. Longitude-velocity diagrams for the galactic plane and for latitude b + or - 0.5 deg are given. The half-thickness of the CO plane, its displacement, and the emissivity of CO, all as functions of galactic radius, are shown. New results are generally consistent with those reported before.

Cohen, R. S.↗

NASA NTRS1982

OH fluorescence in comets - Fluorescence efficiency of the ultraviolet bands

The present investigation is concerned with the calculation of the fluorescence efficiency of OH over a wide range of heliocentric radial velocities. Three vibrational states in each of the two lowest electronic states are included in these calculations, producing a total of nine vibrational bands in the ultraviolet. The attempt is made to reproduce observed, ultraviolet, cometary spectra of OH by utilizing the Swings fluorescence mechanism. The inclusion of four rotational states within each of the three vibrational states and a detailed solar atlas make it possible to match observations very well, although there remain some discrepancies. This ability to reproduce observations provides confidence that the more general results obtained for the fluorescence efficiency as a function of heliocentric radial velocity can be used in interpreting both previous and future observations of OH in comets.

Schleicher, D. G.↗

NASA NTRS1983

A fundamental comparison of canard and conventional configurations

This paper examines the relative efficiency of canard, tandem, and aft-tailed aircraft configurations through analysis of an elementary lifting system. Stability and trim requirements are imposed upon the system and its C sub L(max), drag, and structural weight are studied as its geometry varies over a wide range of possible configurations. During the course of the analysis, two general solutions emerge for minimum induced drag as a function of the geometry and the division of lift between the surfaces of such a system - one for fixed span and the other for fixed weight. The conclusion is that the pre-eminence of the conventional aft-tailed configuration has a sound fundamental basis, but that there may be some room for improvement.

Mcgeer, T.↗

NASA NTRS1984

A performability solution method for degradable nonrepairable systems

The present performability model-solving algorithm identifies performance with 'reward', representing the state behavior of a system S by a finite-state stochastic process and determining reward by means of reward rates that are associated with the states of the base model. A general method is obtained for determining the probability distribution function of the performance (reward) variable, and therefore the performability, of the corresponding system. This is done for bounded utilization periods, and the result is an integral expression which is either analytically or numerically solvable.

Furchtgott, D. G.↗

NASA NTRS1984

Asymmetric spline surfaces - Characteristics and applications

Asymmetric spline surfaces appear useful for the design of high-quality general optical systems (systems without symmetries). A spline influence function defined as the actual surface resulting from a simple perturbation in the spline definition array shows that a subarea is independent of others four or more points away. Optimization methods presented in this paper are used to vary a reflective spline surface near the focal plane of a decentered Schmidt-Cassegrain to reduce rms spot radii by a factor of 3 across the field.

Stacy, J. E.↗

NASA NTRS1985

Test and evaluation plan for the Centralized Storm Information System

The installation of the Centralized Storm Information System (CSIS) at the NOAA operational complex in Kansas City, Missouri is described. This complex includes the National Severe Storms Forecast center and a Satellite Field Service Station which is denoted in this research plan as NSSFC. CSIS computers will act in concert to merge analyze the many data sets needed to forecast severe convective storms. Specific aspects of CSIS are evaluated against the CSIS objectives. The functions to be evaluated characterize the attributes of a generalized interactive computer system. A major development in the CSIS program will allow communication between CSIS and the NSSFC Eclipse computer.

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