Survival of epiphytic bacteria from seed stored on the Long Duration Exposure Facility (LDEF)
Microbial contamination in American spacecraft has previously been documented, however, potential risks to plants and humans in future space based controlled ecological life support systems (CELSS) have yet to be addressed directly. The current study was designed to determine the survival of microorganisms exposed to the relatively harsh conditions found in low Earth orbit (LEO). Total mean dosage for flight and ground control seeds were 210.2 and 0.9 rads, respectively. Bacteria were isolated by plating samples of seedwashings onto dilute tryptic soy agar. Pure isolates of morphologically distinct bacteria were obtained by standard microbiological procedures. Bacteria were grouped according to colony type and preliminary identification was completed using a fatty acid analysis system. Bacillus spp. were the primary microorganisms that survived on seed during the experiment. Results support the hypothesis that terrestrial microorganisms can survive long periods of time in relatively harsh LEO environments.