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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 919 records · Page 51
All sourcesNASA NTRSDOE OSTI
DOE OSTI2018

Rare Earth Elements Alter Redox Balance in Methylomicrobium alcaliphilum 20Z R

Background: Rare Earth Elements (REEs) control methanol utilization in both methane- and methanol-utilizing microbes. It has been established that the addition of REEs leads to the transcriptional repression of MxaFI-MeDH (a two-subunit methanol dehydrogenase, calcium-dependent) and the activation of XoxF-MeDH (a one-subunit methanol dehydrogenase, lanthanumdependent). Both enzymes are pyrroquinoline quinone-dependent alcohol dehydrogenases and show significant homology; however, they display different kinetic properties and substrate specificities. This study investigates the impact of the MxaFI to XoxF switch on the behavior of metabolic networks at a global scale. Results: In this study we investigated the steady-state growth of Methylomicrobium alcaliphilum 20Z R in media containing calcium (Ca) or lanthanum (La, a REE element). We found that cells supplemented with La show a higher growth rate compared to Ca-cultures; however, the efficiency of carbon conversion, estimated as biomass yield, is higher in cells grown with Ca. Three complementary global-omics approaches—RNA-seq transcriptomics, proteomics, and metabolomics—were applied to investigate the mechanisms of improved growth vs carbon conversion. Cells grown with La showed the transcriptional activation of the xoxF gene, a homologue of the formaldehyde-activating enzyme (fae2), a putative transporter, genes for hemin-transport proteins, and nitrate reductase. In contrast, genes for mxaFI and associated cytochrome (mxaG) expression were downregulated. Proteomic profiling suggested additional adjustments of the metabolic network at the protein level, including carbon assimilation pathways, electron transport systems, and the tricarboxylic acid (TCA) cycle. Discord between gene expression and protein abundance changes points toward the possibility of post-transcriptional control of the related systems including key enzymes of the TCA cycle and a set of electron-transport carriers. Metabolomic data followed proteomics and showed the reduction of the ribulosemonophosphate (RuMP) pathway intermediates and the increase of the TCA cycle metabolites. Conclusions: Cells exposed to REEs display higher rates of growth but have lower carbon conversion efficiency compared to cells supplemented with Ca. The most plausible explanation for these physiological changes is an increased conversion of methanol into formate by XoxF-MeDH, which further stimulates methane oxidation but limits both the supply of reducing power and flux of formaldehyde into the RuMP pathway.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Interplanetary Protons versus Interacting Protons in the 2017 September 10 Solar Eruptive Event

We analyze the relativistic proton emission from the Sun during the eruptive event on 2017 September 10, which caused a ground-level enhancement (GLE 72) registered by the worldwide network of neutron monitors. Using the neutron monitor data and interplanetary transport modeling both along and across interplanetary magnetic field (IMF) lines, we deduce parameters of the proton injection into the interplanetary medium. The inferred injection profile of the interplanetary protons is compared with the profile of the >100 MeV γ -ray emission observed by the Fermi Large Area Telescope, attributed to pion production from the interaction of >300 MeV protons at the Sun. GLE 72 started with a prompt component that arrived along the IMF lines. This was followed by a more prolonged enhancement caused by protons arriving at the Earth across the IMF lines from the southwest. The interplanetary proton event is modeled using two sources—one source at the root of the Earth-connected IMF line and another source situated near the solar western limb. The maximum phase of the second injection of interplanetary protons coincides with the maximum phase of the prolonged >100 MeV γ -ray emission that originated from a small area at the solar western limb, below the current sheet trailing the associated coronal mass ejection (CME). A possible common source of interacting protons and interplanetary protons is discussed in terms of proton acceleration at the CME bow shock versus coronal (re-)acceleration in the wake of the CME.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

Direct and semi-direct radiative forcing of biomass-burning aerosols over the southeast Atlantic (SEA) and its sensitivity to absorbing properties: a regional climate modeling study

Simulations are performed for the period 2000-2015 by two different regional climate models, ALADIN and RegCM, to quantify the direct and semi-direct radiative effects of biomass-burning aerosols (BBAs) in the southeast Atlantic (SEA) region. Different simulations have been performed using strongly absorbing BBAs in accordance with recent in situ observations over the SEA. For the July-August-September (JAS) season, the single scattering albedo (SSA) and total aerosol optical depth (AOD) simulated by the ALADIN and RegCM models are consistent with the MACv2 climatology and MERRA-2 and CAMS-RA reanalyses near the biomass-burning emission sources. However, the above-cloud AOD is slightly underestimated compared to satellite (MODIS and POLDER) data during the transport over the SEA. The direct radiative effect exerted at the continental and oceanic surfaces by BBAs is significant in both models and the radiative effects at the top of the atmosphere indicate a remarkable regional contrast over SEA (in all-sky conditions), with a cooling (warming) north (south) of 10°S, which is in agreement with the recent MACv2 climatology. In addition, the two models indicate that BBAs are responsible for an important shortwave radiative heating of ~0.5-1K per day over SEA during JAS with maxima between 2 and 4 km a.m.s.l. (above mean sea level). At these altitudes, BBAs increase air temperature by ~0.2-0.5 K, with the highest values being co-located with low stratocumulus clouds. Vertical changes in air temperature limit the subsidence of air mass over SEA, creating a cyclonic anomaly. The opposite effect is simulated over the continent due to the increase in lower troposphere stability. The BBA semi-direct effect on the lower troposphere circulation is found to be consistent between the two models. Changes in the cloud fraction are moderate in response to the presence of smoke, and the models differ over the Gulf of Guinea. Finally, the results indicate an important sensitivity of the direct and semi-direct effects to the absorbing properties of BBAs. Over the stratocumulus (Sc) region, DRE varies from +0.94 W m -2 (scattering BBAs) to +3.93 W m -2 (most absorbing BBAs).

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2019

Insights into AMS/PCAT transporters from biochemical and structural characterization of a double Glycine motif protease

The secretion of peptides and proteins is essential for survival and ecological adaptation of bacteria. Dual-functional ATP-binding cassette transporters export antimicrobial or quorum signaling peptides in Gram-positive bacteria. Their substrates contain a leader sequence that is excised by an N-terminal peptidase C39 domain at a double Gly motif. We characterized the protease domain (LahT150) of a transporter from a lanthipeptide biosynthetic operon in Lachnospiraceae and demonstrate that this protease can remove the leader peptide from a diverse set of peptides. The 2.0 Å resolution crystal structure of the protease domain in complex with a covalently bound leader peptide demonstrates the basis for substrate recognition across the entire class of such transporters. The structural data also provide a model for understanding the role of leader peptide recognition in the translocation cycle, and the function of degenerate, non-functional C39-like domains (CLD) in substrate recruitment in toxin exporters in Gram-negative bacteria.

Bobeica, Silvia C.↗
NASA NTRS1971

Introduction

Management of earth resources requires accurate knowledge of inventories. Developments of improved remote sensing devices and vehicles for their transport, and automatic data processing equipment and techniques are important management tools for an integrated earth resources survey in California.

Colwell, R. N.↗
NASA NTRS1973

Effect of crossflow velocity on the generation of lift fan jet noise in VTOL aircraft

Analytical studies based on a turbulent mixing noise prediction technique indicate that jet noise power levels are increased when a jet is situated in a crossflow. V/STOL model transport acoustic test data obtained in the NASA Ames 40 ft. x 80 ft. wind tunnel confirmed this jet noise power level increase due to crossflow. Increases up to 6 db at a Strouhal number of 2.5 and crossflow velocity to jet velocity ratio of 0.58 were observed. The power level increases observed in the experimental data confirm the predicted power level increases.

Stimpert, D. L.↗
NASA NTRS1974

Transport dynamics of a high-power-density matrix-type hydrogen-oxygen fuel cell

Experimental transport dynamics tests were made on a space power fuel cell of current design. Various operating transients were introduced and transport-related response data were recorded with fluidic humidity sensing instruments. Also, sampled data techniques were developed for measuring the cathode-side electrolyte concentration during transient operation.

Prokopius, P. R.↗
NASA NTRS1975

The NATA code: Theory and analysis, volume 1

A computer program for calculating quasi-one-dimensional gas flow in axisymmetric and two-dimensional nozzles and rectangular channels is presented. Flow is assumed to start from a state of thermochemical equilibrium at a high temperature in an upstream reservoir. The program provides solutions based on frozen chemistry, chemical equilibrium, and nonequilibrium flow with finite reaction rates. Electronic nonequilibrium effects can be included using a two-temperature model. An approximate laminar boundary layer calculation is given for the shear and heat flux on the nozzle wall. Boundary layer displacement effects on the inviscid flow are considered also. Chemical equilibrium and transport property calculations are provided by subroutines. The code contains precoded thermochemical, chemical kinetic, and transport cross section data for high-temperature air, CO2-N2-Ar mixtures, helium, and argon. It provides calculations of the stagnation conditions on axisymmetric or two-dimensional models, and of the conditions on the flat surface of a blunt wedge. The primary purpose of the code is to describe the flow conditions and test conditions in electric arc heated wind tunnels.

Bade, W. L.↗
NASA NTRS1976

Absorption of radiation belt particles by the inner satellites of Jupiter

The study of trapped particle absorption by the inner Jovian satellites is reviewed from the viewpoint of radiation belt physics. Both pre- and post-Pioneer work is discussed but the emphasis is on methods used to deduce radial diffusion coefficients of particle transport from particle data. The phenomenon of particle absorption as observed by experiments on Pioneers 10 and 11 is considered; absorption effects are found to depend on the satellite, and on particle energy and species. Approximate diffusion coefficients derived from the data are found to follow a steeper spatial dependence than previously expected. The assumptions and limitations of absorption analysis and diffusion coefficient estimation are pointed out.

Mogro-Campero, A.↗
NASA NTRS1977

Prototype solar heating and hot water system

Progress is reported in the development of a solar heating and hot water system which uses a pyramidal optics solar concentrator for heating, and consists of the following subsystems: collector, control, transport, and site data acquisition. Improvements made in the components and subsystems are discussed.

Source record↗
NASA NTRS1980

Simulated transonic flows for aircraft with nacelles, pylons, and winglets

A computational method which simulates transonic flow about wing-fuselage configurations has been extended to include the treatment of multiple body and non-planar wing surfaces. The finite difference relaxation scheme is characterized by a modified small disturbance flow equation and multiple embedded grid system. Wing-body combinations with as many as four nacelles/pods, four pylons, and wing-tip-mounted winglets can be analyzed. A scheme for modeling inlet spillage and engine exhaust interference effects has been included. Computed results are correlated with experimental data for three transport configurations.

Boppe, C. W.↗
NASA NTRS1981

Automated installation of large platform utilities

The capabilities of the assembler in installing nonstructural platform systems were assessed. These systems include electrical power distribution, heat transport, command and data signal transmission, and payload data transmission. The effects of including installation of utilities on the assembly process and on the design, operation, and performance of the assembler were evaluated. In addition, any special requirements on the assembler and/or the platform due to the installation of utilities were identified. The procedure followed was to define a set of candidate utility characteristics and installation requirements, define and design installation concepts, and perform an assessment of impacts on the assembler and its operation.

Vernon, R. M.↗
NASA NTRS1982

Controlled ecological life support system: Transportation analysis

This report discusses a study utilizing a systems analysis approach to determine which NASA missions would benefit from controlled ecological life support system (CELSS) technology. The study focuses on manned missions selected from NASA planning forecasts covering the next half century. Comparison of various life support scenarios for the selected missions and characteristics of projected transportation systems provided data for cost evaluations. This approach identified missions that derived benefits from a CELSS, showed the magnitude of the potential cost savings, and indicated which system or combination of systems would apply. This report outlines the analytical approach used in the evaluation, describes the missions and systems considered, and sets forth the benefits derived from CELSS when applicable.

Gustan, E.↗
NASA NTRS1983

Modeling correlation with flight data

The molecular contamination flow model (space 2 program) predicts the induced environment of the space shuttle orbiter/payload on orbit as well as the induced gas flow between the orbiter and payload surfaces. This computer program relates the input parameters which characterize the time dependent status of the orbiter/payload to the required output parameters. An application of the model to data from space transportation system flights 1, 2, and 3 shows that the model correlates well for direct flow and for return flux (STS-2/H2O). Correlation for return flux from other molecular sources/species appears to be within expectations but more analysis is required. The space 2 model seems to be an adequate predictive tool.

Ehlers, H. K. F.↗
NASA NTRS1984

Transonic analysis of canted winglets

A computational method developed to provide a transonic analysis for upper/lower surface wing-tip mounted winglets is described. Winglets with arbitrary planform, cant and toe angle, and airfoil section can be modeled. The embedded grid approach provides high flow field resolution and the required geometric flexibility. In particular, coupled Cartesian/cylindrical grid systems are used to model the complex geometry presented by canted upper/lower surface winglets. A new rotated difference scheme is introduced in order to maintain the stability of the small-disturbance formulation in the presence of large spanwise velocities. Wing and winglet viscous effects are modeled using a two-dimensional 'strip' boundary layer analysis. Correlations with wind tunnel and flight test data for three transport configurations are included.

Rosen, B. S.↗
NASA NTRS1985

An air-breathing ballistic space transporter for Europe

With increasing transport requirements, reusable space transporters again receive serious consideration in Europe as successors to the Ariane family. The paper deals with a hydrogen-ramjet-propelled, 1-1/2-stage reusable ballistic space transporter with vertical take-off and landing and using liquid hydrogen/oxygen rockets. This novel concept was developed in a theoretical study at the University of Stuttgart. The results are compared with recently published studies of several other European space transporter concepts. The data derived for the Istra - concept are: 15.4 Mg payload into low Earth-orbit, 155 Mg gross lift-off mass, 10% payload ratio, which represents a 57% propellant saving, and 44% reduction in dry mass (structure and engines) compared with comparable two-stage pure rocket concepts.

Kramer, P. A.↗