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At least 919 records · Page 51

PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high‐throughput sequencing

The reconstructed in vitro translation system known as the PURE system has been used in a variety of cell‐free experiments such as the expression of native and de novo proteins as well as various display methods to select for functional polypeptides. We developed a refined PURE‐based display method for the preparation of stable messenger RNA (mRNA) and complementary DNA (cDNA)‐peptide conjugates and validated its utility for in vitro selection. Our conjugate formation efficiency exceeded 40%, followed by gel purification to allow minimum carry‐over of components from the translation system to the downstream assay enabling clean and efficient random peptide sequence screening. We chose the commercially available anti‐FLAG M2 antibody as a target molecule for validation. Starting from approximately 1.7 × 10(exp 12) random sequences, a round‐by‐round high‐throughput sequencing showed clear enrichment of the FLAG epitope DYKDDD as well as revealing consensus FLAG epitope motif DYK(D/L/N)(L/Y/D/N/F)D. Enrichment of core FLAG motifs lacking one of the four key residues (DYKxxD) indicates that Tyr(Y) and Lys (K) appear as the two key residues essential for binding. Furthermore, the comparison between mRNA display and cDNA display method resulted in overall similar performance with slightly higher enrichment for mRNA display. We also show that gel purification steps in the refined PURE‐based display method improve conjugate formation efficiency and enhance the enrichment rate of FLAG epitope motifs in later rounds of selection especially for mRNA display. Overall, the generalized procedure and consistent performance of two different display methods achieved by the commercially available PURE system will be useful for future studies to explore the sequence and functional space of diverse polypeptides.

cDNA display, FLAG epitope, mRNA display, peptide ↗

Assuring Intelligent Systems: Contingency Management for UAS

Unmanned aircraft systems (UAS) collaborate with humans to operate in diverse, safety-critical applications. However, assurance technologies need to be integrated into the design process in order to guarantee safe behavior, thereby enabling UAS operations in the National Airspace System (NAS). In this paper, formal methods are integrated with learning-enabled systems representations. The generation and representation of knowledge are captured via monadic second-order logic rules in the cognitive architecture Soar. These rules are translated into timed automata, and a proof of correctness for the translation is provided so that safety and liveness properties can be checked in the formal verification environment Uppaal. This approach is agnostic to the learning mechanism used to generate the learned rules (e.g., chunking, etc.). An example of a fault-tolerant, learning-enabled UAS deciding which of four contingency procedures to execute under a lost link scenario while overflying an urban area is used to illustrate the approach.

Intelligent Systems↗

The Acoustic Air-Jet Generator

This paper describes the development history, operation, and working principle of an acoustic air-jet generator, which can be used to produce high intensity, high frequency acoustic waves. Flow imaging experiments of the system are shown, and applications of the system for various fluid flows are presented. While previous articles on this system have been written in English by the author, this article provides the only documented explanation and implementation of the focusing schlieren system used for flow imaging of the system. This paper has been cited in early works on focusing schlieren as one of the first implementations of such a system, but has not been widely cited in the bulk of the literature on the focusing schlieren method (aside from a more recent M.S. thesis), perhaps due to the difficulty of accessing the paper or that no translation was available. The purpose of this translation is to provide an English version of the article for researchers interested in this work of Dr. Hartmann, and in particular the description of his focusing schlieren system and its application.

Julius Hartmann↗

Habitable Working and Living Spaces in the Deep Space Science Vessel

The Deep Space Science Vessel (DSSV) is a conceptual design of a very large spacecraft intended as a mobile microgravity science platform. It represents an effort to conceptualize the systems and internal architectures needed to support a forty-eight-person crew for multi-year missions throughout the inner solar system and potentially beyond. The general arrangement of the DSSV is a modular spacecraft. Considering only the pressurized, habitable elements, the DSSV includes two large pressurized modules (Hab and Lab) docked together along with eight smaller node modules completing the habitable configuration. The Hab and Lab modules are docked side by side, with the longitudinal axes parallel to each other and a single docking port connecting them. A node module is docked to each dome on the Hab and Lab modules, such that the two small node modules on each end of the large Hab and Lab domes are docked both to each other and to the Hab and Lab. This creates a “racetrack” translation path on the interior. The other four node modules are docked at the center of the Hab and Lab modules, two on each side, creating another “racetrack” translation path perpendicular to the first one. The DSSV has a generally vertical orientation. Within the Hab and Lab modules the decks are perpendicular to the longitudinal axis. The node modules are oriented with their longitudinal axes perpendicular to those of the Hab and Lab modules, so the nodes have a horizontal internal orientation. Decks are numbered one through nine, with decks two and eight representing the “top” and “bottom” domes of the Hab and Lab modules. The Hab Module decks encompass the ship’s galley, crew quarters, waste, hygiene, portions of crew exercise, and group recreation. The Lab Module contains food production facilities, the life science lab, the ship’s infirmary, the physical science lab, and the maintenance and fabrication workshop. The Node Modules serve a number of different functions. The two Exercise Nodes are docked to deck five of both the Hab and Lab modules and contain most of the aerobic and resistive exercise devices. Also docked to deck five but on the opposite side of the Hab and Lab modules are the Observation Deck and Space Café. These two node modules provide social gathering space for small numbers of crew. The Mission Operations Node is on deck nine directly above the Galley and contains spacecraft monitoring and commanding capabilities. Docked to it is the EVA Operations Node, which contains suit maintenance and storage. (The airlock is a separate, external element docked to the EVA Operations Node.) The Subsystems Node is on deck one beneath the maintenance and fabrication workshop. Docked to it is the Stowage Node. This node does not house DSSV primary stowage, but is a staging point where stowage brought in from logistics modules can be sorted, unpacked, or repacked as needed prior to distribution to the appropriate sections of the spacecraft.

Deep Space Habitat↗

FPP: A Modeling Language for F Prime

We present F Prime Prime (FPP), a new open-source modeling language for F Prime. F Prime is an open-source flight software framework developed at JPL and deployed, among other places, on the Mars helicopter Ingenuity. FPP provides a convenient way to model the architectural elements of an F Prime application, e.g., components, ports, and their connections. It has a succinct and readable syntax, a well- defined semantics, and robust error checking and reporting. The FPP tool suite, written in Scala, analyzes FPP models, reports errors, and translates correct FPP models to a combination of XML and C++. Existing F Prime tools translate the XML to a partial implementation in C++, to be completed by the developers. The model elements have clean interfaces and are highly reusable. An accompanying visualization tool constructs diagrams of components and connections that FSW developers can use to understand and communicate their designs, for ex- ample at reviews. We discuss the design and implementation of FPP and the integration of FPP into F Prime. We also discuss our experience using FPP to construct F Prime models. Finally, we discuss our plans for future work, including improved code generation, improved visualization, and more advanced analysis capabilities.

Starch, Michael D.↗

Fabrication of Gecko Mobility Aids for Deep Space Exploration

Spacecraft large enough for crew to move around inside them have traditionally used handrails and foot restraints to enable crew mobility. The mass of this hardware can become significant in large spacecraft such as ISS or a Mars Transit Habitat. The Common Habitat study also determined that handrails and foot restraints in a multi-gravity habitat are trip hazards when the habitat is in a gravity environment. ISS crew have noted risks of breaking ankles and wrists when using handrails for translation and have noted places where not enough handrails are present. Robotic gecko-derived grippers developed by JPL to retrieve satellites can be adapted to crew-worn pads that can adhere to surfaces to enable crew translation in microgravity. This project will fabricate prototypes of crew clothing augmented with gecko pads.

Restraints and Mobility Aids↗

Next Steps Following Quality Management System Implementation

While Quality Management Systems (QMS) have become increasingly common in industry over the past twenty-five to thirty years, government has been slow to adopt. There are several reasons, including the fact that government is not as tightly focused on specific groups of products as is private industry, and therefore must adapt from a product realization focus to a mission realization focus. This article will present an approach for translating the production-oriented Quality Management Systems Standards, such as those included in ISO 9001, Quality management systems-Requirements and translating them to a mission realization approach. Once a mission approach is developed and understood, it will provide the needed greater clarity and a framework for an agency to implement a QMS. When the QMS is established and implemented, agencies can then take the next steps toward continual improvement. The graphic in Table 1 illustrates the ISO 9001 provisions and the corresponding mission-related concepts and definitions are included in Table 2.

Quality Management System↗

Countermeasures for Mitigation of Sensorimotor Decrements Following Head-Down Bed Rest

BACKGROUND Astronauts experience postflight disturbances in postural and locomotor control due to sensorimotor adaptations during spaceflight. These alterations may have adverse consequences if a rapid egress is required after landing. Although exercise is partially effective for mitigating cardiovascular and muscular deconditioning, additional countermeasures are needed to further preserve sensorimotor function for exploration missions. We have identified proprioceptive training and electrical muscle stimulation (EMS) as promising in-flight countermeasures. Since prolonged head down bed rest (HDBR) is a spaceflight analog for body unloading and causes postural and locomotor control decrements that parallel those observed after spaceflight, it can be used to facilitate the development of these countermeasures. METHODS This study will determine the effects of proprioceptive training and EMS on functional task performance and sensorimotor function following 60 days of 6° HDBR. Subjects will be randomly assigned to one of four groups: 1)an EMS arm, 2) a proprioceptive training arm, 3) an exercise plus proprioceptive training arm, and 4) a control arm. The EMS countermeasure will include daily bilateral stimulation of selected bilateral lower extremity muscles (30 minutes per session). Proprioceptive training will be performed three days per week (20 minutes per session) consisting of body-loaded postural tasks in the horizontal position on an air bearing sled. Exercise training will mimic current protocols used on the International Space Station, but treadmill aerobic exercise will be replaced with additional cycling aerobic exercise. Primary outcome measures will include pre and post HDBR functional tests that are representative of high priority exploration mission tasks and require high demand for dynamic control of postural stability. Additional measures will be used to identify the key physiological factors contributing to countermeasure benefits. Given the constrained samples size, a Bayesian modelling approach will be used to quantify the probability that there is an effect of a given magnitude. HARDWARE AND PROTOCOL DEVELOPMENT Proprioceptive countermeasure design enhancements continued as part of the Mars Campaign Office (MCO) Crew Health and Performance (CHP) Crew Health Countermeasures (CHC). The primary goals of this work were to assemble a portable version of the countermeasure system that can be used at the :envihab facility, expand the software feedback system’s capabilities, and develop an actuator loading system that mimics what could be used on the International Space Station. In addition, one major piece of exercise hardware used in previous HDBR studies, the Horizontal Squat Device, was reassembled and restored to full functionality. Human-in-the-loop pilot testing is ongoing, prior to shipping both devices to the :envihab HDBR facility. Evaluations are also underway for the best EMS device technologies and specific methods. Finally, assessment techniques are being translated for administration in the horizontal position (e.g., leg dexterity and foot sole skin sensitivity). Our current goals are to refine the countermeasure training and assessment techniques and integrate protocols across multiple modalities. RELEVANCE The deliverable from this project will be proof-of-concept sensorimotor countermeasure designs for functional task performance with full assessment of efficacy in a spaceflight analog. If one or more countermeasures are effective, they will be translated for validation with the suite of operationally implemented in-flight countermeasures. ACKNOWLEDGEMENT This work is supported by NASA’s Human Research Program Human Health Countermeasures Element and by the Canadian Space Agency (L. Bent).

T R Macaulay↗

Conversion of Environment Files from ORDEM 3 & MEM 3 to STENVI

To perform Micrometeoroid and Orbital Debris (MMOD) analyses, NASA describes the orbital debris particle environment using the ORDEM 3 tool and the micrometeoroid environment using the MEM 3 tool. Both output files that detail the flux of particles as a function of several parameters. European MMOD analyses use different tools to perform similar functions, including the Meteoroid And Space debris Terrestrial Environment Reference (MASTER) environment model, which outputs flux data in the Standard Environment Interface (STENVI) format. The NASA Johnson Space Center Hypervelocity Impact Technology Group was asked to create a converter to translate flux data from the output file formats used by ORDEM 3.x and MEM 3 into the STENVI file format. Validation was performed to ensure that the converter was reproducing the input data correctly. The Bumper 3 MMOD risk assessment tool has a capability for reading ORDEM 3.x, MEM 3, and STENVI environment file formats and was used to compare MMOD risk results generated using the converter’s output STENVI files to the risk results generated using the input ORDEM 3.x and MEM 3 igloo files. Specific point comparisons validated that the converter translated the environment data correctly, producing the intended output. However, realistic risk assessments showed that the bin methodology employed by Bumper for determining particle flux as a function of particle size when reading files in STENVI format produces much higher MMOD risk than the point-interpolation methodology used in Bumper risk assessments using ORDEM and MEM. Modifications to how Bumper 3 reads STENVI files during analyses are considered to improve Bumper’s ability to perform useful analyses with environment files in this format.

STENVI↗

Manual Crew Override of Vehicle Landings Following G-Transitions

BACKGROUND Manual control during exploration spaceflight consists of both planned automated supervisory control and unplanned crew override. This crew override capability is critical to enable overall mission success during landing contingencies. However, the introduction of manual override capabilities must be implemented to enable crews to mitigate risks introduced by human error. Adaptive changes in the sensorimotor system can manifest during g-transitions as spatial disorientation. While training and landing aids enable successful landing through disorientation, these adaptive changes may increase cognitive demand that needs to be accounted for in the manual control strategy. It is important to characterize these effects as soon as possible following the G-transition to develop appropriate countermeasures. METHODS In this study, we will examine operational piloting tasks following International Space Station (ISS) missions in a simulated lunar landing using a six-degree-of-freedom (6DOF) motion base. The motion-based simulation will be implemented in our laboratory at the Johnson Space Center (JSC) and will be available within hours following the return from commercial crew landings. The primary goals of this study are (1) to understand the impact of spaceflight on crew ability to perform manual crew override tasks, (2) to examine how adaptive changes in vestibular and cognitive function relate to changes in manual crew override proficiency, and (3) compare performance during late “just-in-time” on-board training with early post-flight crew performance. The impact of spaceflight on piloting capability will be assessed from pre- versus post-flight changes in crewmembers assigned to either short duration (< 30 day) or long duration (~6- month) missions to the International Space Station (ISS). Individual differences in post-flight vestibular and cognitive changes include motion sickness reports, measures of tilt motion perception accuracy and precision, and dual-task tracking. During the 6DOF lunar simulation, the crew will manually takeover attitude and rate-of-descent to the nominal or re-designated landing aim point during the approach phase. The outcome measures for the lunar crew override tasks will be the percent time maintaining actual vehicle states, e.g., attitude and rate-of-descent, within recommended guidance during the landing approach, number and maximum deviation outside limits, and root mean square error (RMSE). Given that “just-in-time” (JIT) training is an operational expectation for the Human Landing System (HLS) program, all participants will perform late inflight JIT training for each manual crew override task in which they will participate. Crew proficiency will be captured inflight during JIT training that will be implemented on a laptop with hand controllers to allow the crewmember to practice the landing task procedures like the approach implemented for JIT training with Shuttle landing and ISS telerobotic tasks. COUNTERMEASURE UPDATES The lunar lander simulation has been implemented onto a two-laptop display system where subjects select from alternative landing points, based upon avoiding hazards that the on-board system identifies during the landing. The subject makes inputs using a rotational hand controller (i.e., joystick) and a translational hand controller, which are processed by simulated vehicle dynamics to update the vehicle attitude and rate of descent. Subjects will utilize a combination of flight, situation, and status displays to monitor the state of the simulated vehicle. The simulation exports tilt and translation movements to a 6DOF motion base synced to visual movements and provides representative vestibular cues to the subject while performing the task inside an enclosed cabin featuring a simulated lunar out-the-window view and a heads-down lander cockpit view. RELEVANCE This project will deliver an operational demonstration of crew override capability following spaceflight and identify potential deficits that may require remediation. Comparison of individual vestibular and cognitive changes with crew override performance will help better characterize the manual control risks associated with sensorimotor alterations. The inclusion of “just-in-time” on-board training will ensure we are characterizing changes in override proficiency with this expected countermeasure in place. ACKNOWLEDGEMENTS: The authors acknowledge contributions from Draper, HLS Joint Test Panel, Crew HLS Interfaces for Piloting Working Group, and Dynamic Skills Trainer (DST) Lab toward the development of the lunar landing simulation. This project is funded by the Human Health Countermeasures Element.

Austin M Bollinger↗

Expanding Impact with the James Webb Space Telescope: Combining Art, Science, and Technology for Public Outreach

This archived content may contain outdated information or references that may not reflect current policy or programs. NASA’s James Webb Space Telescope revealed its first images on July 12, 2022 and has been used for groundbreaking science since. Scientific exploration transcends international borders, making a mission like Webb feasible with collaborators including the European Space Agency (ESA), and Canadian Space Agency (CSA). Communication about NASA’s discoveries about the universe, so essential for involving the public, likewise needs to be inclusive to all. Art is a tool for cutting across the boundaries of language, culture, and ability, and serves as a bridge between the technical and non-technical communities. Art makes the beauty and nuance of science and engineering accessible in ways that may not be readily perceived to those outside of those communities of practice. Equitable and inclusive outreach endeavors are challenging to implement while considering language translations, culture context, sensory methods, and technology capabilities. Formats including “Unfolding the Universe'' virtual reality platform has allowed for a wider audience to interact with the Webb images including sounds, visual aids, and talks by the scientists and engineers who worked on Webb. This VR platform has been expanded to highlight the ESA contributors to the mission and will be showcased internationally. The Webb first images were also translated into sounds as a method of inclusion for anyone with sight disabilities. We will explore some successful outreach methods and suggestions for inclusivity in future.

Outreach↗

Conversion of ORDEM 3.X & MEM 3 Into STENVI File Format

To perform Micrometeoroid and Orbital Debris (MMOD) analyses, NASA describes the orbital debris particle environment using the ORDEM 3 tool and the micrometeoroid environment using the MEM 3 tool. Both output files that detail the flux of particles as a function of several parameters. European MMOD analyses use different tools to perform similar functions, including the Meteoroid And Space debris Terrestrial Environment Reference (MASTER) environment model, which outputs flux data in the Standard Environment Interface (STENVI) format. The NASA Johnson Space Center Hypervelocity Impact Technology Group was asked to create a converter to translate flux data from the output file formats used by ORDEM 3.x and MEM 3 into the STENVI file format. Validation was performed to ensure that the converter was reproducing the input data correctly. The Bumper 3 MMOD risk assessment tool has a capability for reading ORDEM 3.x, MEM 3, and STENVI environment file formats and was used to compare MMOD risk results generated using the converter’s output STENVI files to the risk results generated using the input ORDEM 3.x and MEM 3 igloo files. Specific point comparisons validated that the converter translated the environment data correctly, producing the intended output. However, realistic risk assessments showed that the bin methodology employed by Bumper for determining particle flux as a function of particle size when reading files in STENVI format produces much higher MMOD risk than the point-interpolation methodology used in Bumper risk assessments using ORDEM and MEM. Modifications to how Bumper 3 reads STENVI files during analyses are considered to improve Bumper’s ability to perform useful analyses with environment files in this format.

STENVI↗

Equitable and Inclusive Public Outreach with the James Webb Space Telescope: Combining Art, Science, and Technology

This archived content may contain outdated information or references that may not reflect current policy or programs. NASA’s James Webb Space Telescope revealed its first images on July 12, 2022 and has been used for groundbreaking science since. Scientific exploration transcends international borders, making a mission like Webb feasible with collaborators including the European Space Agency (ESA), and Canadian Space Agency (CSA). Communication about NASA’s discoveries about the universe, so essential for involving the public, likewise needs to be inclusive to all. Art is a tool for cutting across the boundaries of language, culture, and ability, and serves as a bridge between the technical and non-technical communities. Art makes the beauty and nuance of science and engineering accessible in ways that may not be readily perceived to those outside of those communities of practice. Equitable and inclusive outreach endeavors are challenging to implement while considering language translations, culture context, sensory methods, and technology capabilities. Formats including “Unfolding the Universe'' virtual reality platform has allowed for a wider audience to interact with the Webb images including sounds, visual aids, and talks by the scientists and engineers who worked on Webb. This VR platform has been expanded to highlight the ESA contributors to the mission and will be showcased internationally. The Webb first images were also translated into sounds as a method of inclusion for anyone with sight disabilities. We will explore some successful outreach methods and suggestions for inclusivity in future.

Outreach↗

A Green Propulsion Dual Mode (GPDM) In-Space Technology Demonstration on a 6U CubeSat

Over the past decade there has been an increase in the launch and operations of small spacecraft. Small spacecraft (defined as having a total vehicle mass of < 180 kg) provide an opportunity for low-cost, high impact technology demonstrations on a small scale. They also create opportunities for government, industry, and academic to engage, transfer knowledge, and extend partnerships with non-traditional partners. NASA’s Marshall Space Flight Center (MSFC) is leading a collaborative effort to demonstrate a dual-mode (chemical and electrospray) propulsion system using a common propellant system as a payload on a 6U CubeSat. The novel feature in the propulsion system is the interfacing of propulsion technologies requiring dramatically different operating conditions and support hardware. GPDM will use a low toxicity or “green” propellant known as Advanced Spacecraft Energetic Non-Toxic (ASCENT) to demonstrate using a chemical thruster for translation burns and electrospray propulsion for both attitude control and translational burns on the spacecraft. Specific mission activities could include demonstration of collision avoidance during frequent altitude adjustment maneuvers to validate thruster performance and managing extended duration thruster burns of at least 24 hours with limited vehicle contact times. This paper will summarize the ground testing, spacecraft development, mission objectives, and mission planning activities to achieve some of GPDM’s technical objectives.

Nehemiah Joel Williams↗

Equitable and Inclusive Public Outreach with the James Webb Space Telescope: Combining Art, Science, and Technology

This archived content may contain outdated information or references that may not reflect current policy or programs. NASA’s James Webb Space Telescope revealed its first images on July 12, 2022 and has been used for groundbreaking science since. Scientific exploration transcends international borders, making a mission like Webb feasible with collaborators including the European Space Agency (ESA), and Canadian Space Agency (CSA). Communication about NASA’s discoveries about the universe, so essential for involving the public, likewise needs to be inclusive to all. Art is a tool for cutting across the boundaries of language, culture, and ability, and serves as a bridge between the technical and non-technical communities. Art makes the beauty and nuance of science and engineering accessible in ways that may not be readily perceived to those outside of those communities of practice. Equitable and inclusive outreach endeavors are challenging to implement while considering language translations, culture context, sensory methods, and technology capabilities. Formats including “Unfolding the Universe'' virtual reality platform has allowed for a wider audience to interact with the Webb images including sounds, visual aids, and talks by the scientists and engineers who worked on Webb. This VR platform has been expanded to highlight the ESA contributors to the mission and will be showcased internationally. The Webb first images were also translated into sounds as a method of inclusion for anyone with sight disabilities. We will explore some successful outreach methods and suggestions for inclusivity in future.

Outreach↗

EVA Planning: Using Neutral Buoyancy Laboratory (NBL) Training to Predict in-Flight Energy Expenditure

Metabolic rate (“met rate”) is the amount of energy expended over a period of time and is influenced by many factors including body composition, level of physical activity, resting metabolic rate, sex, age, and food intake. Met rate is measured during Extravehicular Activity (EVA) training at the Neutral Buoyancy Laboratory (NBL) and during in-flight EVAs through indirect calorimetry, calculating energy expenditure from respiratory measurements of O 2 consumption and/or CO 2 production. During Extravehicular Activity (EVA) planning, metabolic cost is important to consider and is used to inform EVA duration based on spacesuit consumables associated with life support systems. Currently, NBL and previous ISS EVA met rate data for specified crewmembers are utilized to predict in-flight EVA metabolic costs based on a proposed EVA timeline. Timeline data collected during training is used to relate met rates to specific EVA activities, which are in turn assigned to more generalized EVA task categories, categorizing by both task type and restraint type. EVA task categories include EVA Setup/Cleanup, Worksite Setup/Cleanup, Cable Routing, Bolts, Fluid Connectors, Electrical Connectors, R&R Work, Miscellaneous Work, Incapacitated Crew Rescue (being rescued or performing), Assisted Crew Rescue (being assisted or performing), and Translation. Restraint types consist of Free-Float, Body Restraint Tether (BRT), Articulating Portable Foot Restraint (APFR), and Space Station Remote Manipulator System (SSRMS). From a crewmember’s historical data, individualized 10th, 50th and 90th percentile met rate estimates are generated for each task category and used to estimate the proposed EVA timeline metabolic cost. In-flight metabolic data (“As-Executed”) from recent ISS US EVAs 85-88 (totaling eight EVA crewmember met rates) was compared with their predicted metabolic cost (“As-Planned”) to evaluate the accuracy of the current met rate estimation method. Across the four EVAs, As-Executed Cumulative EVA Total Metabolic Cost (M = 5893.78 BTU, SD = 816.60) was not significantly different compared to As-Planned Cumulative EVA Total Metabolic Cost (M = 6106.60 BTU, SD = 826.62; t(7) = 0.751 , p = .477). Though not a significant difference, generally, As-Planned total estimates were slightly higher than As-Executed total metabolic cost. Relative Error for Cumulative EVA Total Metabolic Cost ranged from -33% to 14.7%, depending on the crewmember and EVA. When comparing As-Planned to A-Executed EVA task categories for Bolts, Electrical Connectors, EVA Cleanup, EVA Setup, Miscellaneous Work, Translation, Worksite Cleanup, and Worksite Setup during these EVAs, no significant differences were observed, however, there was a significant difference in As-Planned (M = 783.71 BTU, SD = 408.88) compared to As-Executed (M = 608.87 BTU, SD = 425.29) metabolic cost for the task category of Repair-and-Replace (R&R) Work (t(17) = 3.21 , p = .005). Looking closer within the R&R Work task category, As-Executed R&R Work with Free-Float restraint type (M = 706.17 BTU, SD = 352.18) was significantly less than As-Planned R&R Work with Free-Float restraint type (M= 887.96 BTU, SD = 381.42; t(12) = 2.59, p < .024). As-Executed R&R Work with SSRMS Restraint type (M = 355.89 BTU, SD = 534.65) was not significantly different from As-Planned values (M = 512.69 BTU, SD = 383.34; t(4) = 1.88, p = 0.132). These findings suggest that the energy expended performing R&R Work (Free-Float) is lower in flight than predicted. Accurate predictions of the metabolic cost of EVA are essential for planning and executing successful ISS EVAs. Overall, the current met rate prediction method is similar to actual in-flight values, slightly erring on the side of overestimation. Future work includes analysis of more historical in-flight EVA data to increase the power of the analysis, evaluating the NBL-ISS met rate conversion factor between NBL and ISS tasks, as well as exploring methods of substitution when crewmembers are missing prior task category data.

Lauren Cox↗

Improvements to Stitching Controls for Manufacturing Advanced Stitched Composites

Stitched composites have been extensively studied and have been shown to have benefits over unstitched composites for stiffened structures, including improved damage tolerance, reduced weight, and fewer fasteners. These benefits make stitched composites attractive from a performance perspective, however, the nature of stitching the preforms prior to infusion includes additional manufacturing steps and equipment compared to conventional resin infusion. Stitching of composite material structures at NASA Langley Research Center (LaRC) is performed at the Integrated Structural Assembly of Advanced Composites (ISAAC) facility using control algorithms developed by the lead author. Two deficiencies have been identified in the current control algorithms, and mitigations for these deficiencies were developed within the Hi-Rate Composite Aircraft Manufacturing (HiCAM) project and are presented herein. First, decoupling stitch translation and rotation takes more time for stitching since two robot operation control steps are executed for each stitch. Therefore, a method was developed to determine when to decouple the translation and rotation for stitching, which reduces stitch time by decoupling the operation control steps only when necessary to execute the stitch properly. Second, a correction to the sideslip stitching approach that eliminates a significant portion of the pre- and post-stitching activities was developed. The developed method adjusts the sideslip stitch angle based on the rotations of previous stitches to ensure that the width of the stitching seam remains constant along its length. Constant seam width eliminates observed overlap of the insertion and catcher thread portions for highly curved paths, which maintains the desired uniformity of through-the-thickness architecture along the seam and eliminates the local degradation of properties where the insertion needle is too close to, or crosses, the catcher needle portion of the seam.

stitching↗