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At least 919 records · Page 51

Photoluminescence recovery by in-situ exposure of plasma-damaged n-GaN to atomic hydrogen at room temperature

The effect of in-situ exposure of n-GaN damaged by Cl{sub 2} plasma to atomic hydrogen (H radicals) at room temperature was investigated. We found that the PL intensities of the band-edge emission, which had been drastically reduced by plasma-beam irradiation at a Cl ion dose of 5 Multiplication-Sign 10{sup 16} cm{sup -2}, recovered to values close to those of as-grown samples after H radical exposure at a dose of 3.8 Multiplication-Sign 10{sup 17} cm{sup -2}. XPS revealed the appearance of a peak at a binding energy of 18.3 eV, which is tentatively assigned to Ga-H, and confirmed the removal of Cl after H radical exposure.

36 MATERIALS SCIENCE↗

Use of molecular beacons for the rapid analysis of DNA damage induced by exposure to an atmospheric pressure plasma jet

A rapid method for evaluating the damage caused to DNA molecules upon exposure to plasma is demonstrated. Here, we propose the use of a molecular beacon for rapid detection of DNA strand breaks induced by atmospheric pressure plasma jet (APPJ) irradiation. Scission of the molecular beacon by APPJ irradiation leads to separation of the fluorophore-quencher pair, resulting in an increase in fluorescence that directly correlates with the DNA strand breaks. The results show that the increase in fluorescence intensity is proportional to the exposure time and the rate of fluorescence increase is proportional to the discharge power. This simple and rapid method allows the estimation of DNA damage induced by exposure to a non-thermal plasma.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Survival Rates of Out-Migrating Yearling Chinook Salmon in the Lower Columbia River and Plume after Exposure to Gas-Supersaturated Water

Abstract In 2011, unusually high flows caused total dissolved gas (TDG) levels in the Columbia River, USA, to escalate well above the 120% regulatory limit that was imposed to prevent harmful impacts to aquatic organisms. After observing gas bubble trauma (GBT) in dead yearling Chinook Salmon Oncorhynchus tshawytscha (smolts) held in tanks, we compared estimated survival rates of acoustic-tagged in-river-migrating (IR) and transported (TR) smolts that were released below Bonneville Dam prior to and during the period of elevated TDG (>120%). The log odds of estimated daily survival in the lower river and plume was significantly lower for IR smolts that were released during elevated TDG (maximum possible exposure = 134%) than for IR smolts released when TDG was less than 120%. The TR smolts that were released 10–13 km below Bonneville Dam during elevated TDG had lower maximum possible exposure levels (126% TDG), and the log odds of estimated daily survival in the lower river and plume did not differ from that of TR smolts released when TDG was less than 120%. Direct mortality due to GBT is probably reduced in natural settings relative to laboratory experiments because smolts can move to deeper water, where pressure keeps gasses in solution, and can migrate downstream of the spillway, where TDG levels decrease as the river returns to equilibrium with the atmosphere. However, initially nonlethal GBT may reduce survival rates by increasing smolt susceptibility to predation and infection. Although our findings are limited by the observational nature of the study, our analysis is the first direct assessment of gas supersaturation's potential influence on survival of free-ranging smolts in the river and coastal ocean below a large dam. Experiments using simultaneous releases of control and gas-exposed groups are warranted and should consider the possibility that the chronic effects of TDG exposure on survival are important and persist into the early marine period. Received October 5, 2015; accepted August 18, 2016 Published online October 24, 2016

Brosnan, Ian G. (ORCID:0000000325094325)↗

Systematic reviews and meta-analyses of human and animal evidence of prenatal diethylhexyl phthalate exposure and changes in male anogenital distance

Background: In July 2017, the National Academies released a report applying systematic review (SR) methods in the evaluation of low dose endocrine effects including an assessment of male reproductive toxicity following in utero phthalate exposure. Objective: We evaluated human and animal evidence of the effect of in utero exposure to diethylhexyl phthalate (DEHP) on anogenital distance (AGD) in males. Methods: PubMed, Embase, and Toxline were searched for observational studies in humans and experimental studies in non-human mammals on August 15 2016. Two trained staff independently screened search results, assessed risk of bias for individual studies, and extracted data sequentially. Confidence in the human and animal bodies of evidence was assessed and hazard conclusions reached by integrating evidence streams. Results: The search yielded 24 relevant studies. Meta-analysis of five human observational prospective cohort studies showed consistent evidence that increased maternal urinary concentrations of DEHP metabolites were associated with decreased AGD in male offspring (-4.07 [CI, -6.49 to -1.66] percent decrease per log10 increase in DEHP metabolites). Meta-analysis and meta-regression of 19 experimental animal studies similarly found consistent evidence of decreased AGD with DEHP treatment, with a dose-response gradient, with heterogeneity explained by species and strain. Conclusions: There is a moderate level of evidence from human studies and a high level of evidence from animal studies that in utero exposure to DEHP decreases AGD. Based on the available human and animal evidence, and consideration of mechanistic data, DEHP is presumed to be a reproductive hazard to humans.

Dorman, David C.↗

UTILIZATION OF THE WAVEFRONT SENSOR AND SHORT-EXPOSURE IMAGES FOR SIMULTANEOUS ESTIMATION OF QUASI-STATIC ABERRATION AND EXOPLANET INTENSITY

Heretofore, the literature on exoplanet detection with coronagraphic telescope systems has paid little attention to the information content of short exposures and methods of utilizing the measurements of adaptive optics wavefront sensors. This paper provides a framework for the incorporation of the wavefront sensor measurements in the context of observing modes in which the science camera takes millisecond exposures. In this formulation, the wavefront sensor measurements provide a means to jointly estimate the static speckle and the planetary signal. The ability to estimate planetary intensities in as little as a few seconds has the potential to greatly improve the efficiency of exoplanet search surveys. For simplicity, the mathematical development assumes a simple optical system with an idealized Lyot coronagraph. Unlike currently used methods, in which increasing the observation time beyond a certain threshold is useless, this method produces estimates whose error covariances decrease more quickly than inversely proportional to the observation time. This is due to the fact that the estimates of the quasi-static aberrations are informed by a new random (but approximately known) wavefront every millisecond. The method can be extended to include angular (due to diurnal field rotation) and spectral diversity. Numerical experiments are performed with wavefront data from the AEOS Adaptive Optics System sensing at 850 nm. These experiments assume a science camera wavelength {lambda} of 1.1 {mu}, that the measured wavefronts are exact, and a Gaussian approximation of shot-noise. The effects of detector read-out noise and other issues are left to future investigations. A number of static aberrations are introduced, including one with a spatial frequency exactly corresponding the planet location, which was at a distance of Almost-Equal-To 3{lambda}/D from the star. Using only 4 s of simulated observation time, a planetary intensity, of Almost-Equal-To 1 photon ms{sup -1}, and a stellar intensity of Almost-Equal-To 10{sup 5} photons ms{sup -1} (contrast ratio 10{sup 5}), the short-exposure estimation method recovers the amplitudes' static aberrations with 1% accuracy, and the planet brightness with 20% accuracy.

79 ASTRONOMY AND ASTROPHYSICS↗

SPATIAL AND TEMPORAL DISTRIBUTIONS OF TRANSIENT HORIZONTAL MAGNETIC FIELDS WITH DEEP EXPOSURE

We obtained a long-exposure vector magnetogram of the quiet Sun photosphere at the disk center with a wide field of view of 51'' x 82''. The observation was performed at Fe I 525.0 nm with the shutterless mode of the Narrow Band Filter Imager of the Solar Optical Telescope (SOT) on board the Hinode satellite. We summed the linear polarization (LP) maps taken with a time cadence of 60 s for 2 hr to obtain a map with as long of an exposure as possible. The polarization sensitivity would be more than 4.6 (21.2 in exposure time) times the standard observation with the SOT Spectropolarimeter. The LP map shows a cellular structure with a typical scale of 5''-10''. We find that the enhanced LP signals essentially consist of the isolated sporadic transient horizontal magnetic fields (THMFs) with a lifetime of 1-10 minutes and are not contributed by long-duration weak horizontal magnetic fields. The cellular structure coincides in position with the negative divergence of the horizontal flow field, i.e., mesogranular boundaries with downflows. Azimuth distribution appears to be random for the scale size of the mesogranules. Some pixels have two separate appearances of THMFs, and the measured time intervals are consistent with the random appearance. THMFs tend to appear at the mesogranular boundaries, but appear randomly in time. We discuss the origin of THMFs based on these observations.

79 ASTRONOMY AND ASTROPHYSICS↗

Citizen and machine learning-aided high-resolution mapping of urban heat exposure and stress

Abstract Through conversion of land cover to more built-up, impervious surfaces, cities create hotter environments than their surroundings for urban residents, with large differences expected between different parts of the city. Existing measurements of ambient air temperature and heat stress, however, are often insufficient to capture the intra-urban variability in heat exposure. This study provides a replicable method for modeling air temperature, humidity, and moist heat stress over the urban area of Chapel Hill while engaging citizens to collect high-temporal and spatially-resolved air temperature and humidity measurements. We use low-cost, consumer-grade sensors combined with satellite remote sensing data and machine learning to map urban air temperature and relative humidity over various land-cover classes to understand intra-urban spatial variability of ambient heat exposure at a relatively high resolution (10 m). Our findings show that individuals may be exposed to higher levels of air temperature and moist heat stress than weather station data suggest, and that the ambient heat exposure varies according to land cover type, with tree-covered land the coolest and built-up areas the warmest, and time of day, with higher air temperatures observed during the early afternoon. Combining our resulting dataset with sociodemographic data, policymakers and urban planners in Chapel Hill can use data output from this method to identify areas exposed to high temperature and moist heat stress as a first step to design effective mitigation measures.

54 ENVIRONMENTAL SCIENCES↗

ATM–dependent phosphorylation of CHD7 regulates morphogenesis-coupled DSB stress response in fetal radiation exposure

Following radiation exposure, unrepaired DNA double-strand breaks (DSBs) persist to some extent in a subset of cells as residual damage; they can exert adverse effects, including late-onset diseases. In search of the factor(s) that characterize(s) cells bearing such damage, we discovered ataxia-telangiectasia mutated (ATM)-dependent phosphorylation of the transcription factor chromodomain helicase DNA binding protein 7 (CHD7). CHD7 controls the morphogenesis of cell populations derived from neural crest cells during vertebrate early development. Indeed, malformations in various fetal bodies are attributable to CHD7 haploinsufficiency. Following radiation exposure, CHD7 becomes phosphorylated, ceases promoter/enhancer binding to target genes, and relocates to the DSB-repair protein complex, where it remains until the damage is repaired. Thus, ATM-dependent CHD7 phosphorylation appears to act as a functional switch. As such stress responses contribute to improved cell survival and canonical nonhomologous end joining, we conclude that CHD7 is involved in both morphogenetic and DSB-response functions. Thus, we propose that higher vertebrates have evolved intrinsic mechanisms underlying the morphogenesis-coupled DSB stress response. In fetal exposure, if the function of CHD7 becomes primarily shifted toward DNA repair, morphogenic activity is reduced, resulting in malformations.

59 BASIC BIOLOGICAL SCIENCES↗

Impact of developmental methylmercury exposure on avian embryonic development, hatchling growth, and survival

Abstract Methylmercury (MeHg) is a globally ubiquitous and persistent environmental toxicant that negatively affects animal behavior, health, and reproduction. In birds, MeHg is transferred from female to egg, contaminating embryos during sensitive stages of early embryonic development and growth. This toxic exposure in the prenatal environment not only induces mortality but also possible lasting impacts on physiology, health, and survival, even once hatched. The purpose of our study was to further elucidate the negative effects of MeHg exposure during avian embryonic development and explore how such exposure can impact offspring development, growth, and survival, both in ovo and posthatch. To assess this, we experimentally dosed fertile mallard and wood duck eggs with MeHg II chloride and reared developing embryos and ducklings to various endpoints. We found that embryos not only readily accumulated MeHg throughout incubation, but they also displayed varying dose-dependent disparities in body mass and morphometrics, with control individuals being larger during early and late embryonic stages of development (p < 0.05). Furthermore, hatched offspring exposed to MeHg exhibited increasingly slower growth rates between 7 to 10 and 10 to 15 days posthatch (p < 0.05), and were found to have lower survival probabilities both under controlled laboratory conditions (p < 0.005), and in the natural environment (p < 0.05). Our findings on the detrimental effects of MeHg on avian embryos and hatchlings emphasize the need for more proactive means of environmental protection and remediation to protect vulnerable avian populations and the ecosystems they inhabit.

Leaphart, James C.↗

Dose Coefficients for Internal Dose Assessments for Exposure to Radioactive Fallout

This paper presents values as well as the bases for calculating internal dose coefficients suitable for estimating organ doses from the exposure to radioactive fallout that could result from the detonation of a nuclear fission device. The 34 radionuclides discussed are the same as those given in a priority list of radionuclides for fallout dose assessments presented in a companion overview paper. The radionuclides discussed are those that are believed to account for a preponderance of the organ doses that might be received by intake by persons of all ages (including in utero and via breast feeding for infants) following exposure to radioactive fallout. The presented dose coefficients for ingestion account for age and include modifications for variations in solubility with distance as discussed previously in the literature, and those for inhalation similarly account for age, solubility, and particle sizes that would be relevant at various distances of exposure as discussed in a companion paper on ingestion dose methods. The proposed modifications peculiar to radioactive fallout account for systematic changes in solubility and particle sizes with distance from the site of detonation, termed here as the region of “local fallout” and the region “beyond local fallout.” Brief definitions of these regions are provided here with more detailed discussion in a companion paper on estimating deposition of fallout radionuclides. This paper provides the dose coefficients for ingestion and inhalation (for particle sizes of 1 μm, 5 μm, 10 μm, and 20 μm) for the region “local fallout.” These dose coefficients for “local fallout” are specific for particles formed in a nuclear explosion that can be large and have radionuclides, particularly the more refractory ones, distributed throughout the volume where the radionuclide has reduced solubility. The dose coefficients for the region “beyond local fallout” are assumed to be the ones published by the International Commission on Radiological Protection (ICRP) in 1995. Comparisons of the presented dose coefficients are made with values published by the ICRP.

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTAN↗

Biological Consequences and Health Risks Of Low-Level Exposure to Ionizing Radiation: Commentary on the Workshop

This paper provides an integration and discussion of the information presented at the workshop held from May 2 to 5, 2010, in Richland, WA, adjacent to the Pacific Northwest National Laboratory (PNNL). Consequently, this is commentary and not necessarily a consensus document. This workshop was in honor of Dr. Victor P. Bond in celebration of his numerous contributions to the radiation sciences. Internationally recognized experts in biophysics, experimental radiation biology, epidemiology, and risk assessment were invited to discuss all issues of low-dose risk. This included the physics of track structure and its consequences to dosimetry, primary and secondary responses at the molecular, cellular, and tissue biology levels, epidemiology, definitions of risk, and the practical and regulatory applications of these issues including their biomedical and social consequences. Of major concern was the present state of knowledge about cancer risk and other risks in humans following intentional or accidental exposures to low doses and low dose-rates of ionizing radiation (below about 100 mSv accumulated dose). This includes low dose exposures which occur during radiation therapy in tissues located outside of the irradiated volume. The interdisciplinary approach of this workshop featured discussions rather than formal presentations in ten separate consecutive sessions. Each session was led by chairpersons, listed in the opening of the workshop, which introduced topics, facts and posed relevant questions. The content of each session is given by a brief summary followed by the abstracts from the primary discussants in the session as has been presented in the previous section. This manuscript provides additional review and discussion of the sessions and tracks the topics and issues discussed as follows: • Energy deposition through particle tracks in tissues. • Energy deposition and primary effects in tissues. • Consequences of experimental advances in radiobiology • Non-targeted radiation effects. • System biological considerations. • Propagation of perturbations in the system. • Immediately operating protections. • Delayed stress response protections • Low-dose induced adaptive protections. • Integrated defenses against cancer. • Endogenous versus radiogenic cancer. • The epidemiological dilemma. • Dose-risk functions for different exposure modalities. • Implications for research. • Implications for regulation and protection. A brief summary of the discussions and results on each of these topics and issues is presented in this paper. Additional details of these discussions are provided in the workshop session summaries grouped into topics and followed by applicable abstracts/synopses submitted by the workshop participants.

Feinendegen, Ludwig E.↗

The influence of exposure to early-life adversity on agency-modulated reinforcement learning

Agency beliefs influence how humans learn from different contexts and outcomes. Research demonstrates that stressors, such as exposure to early-life adversity (ELA), are associated with both agency beliefs and learning, but how these processes interact remains unclear. The current study investigated whether exposure to ELA influences agency and interacts with reinforcement learning in adults. Replicating prior behavioral and computational work, ELA resulted in decreased learning, while increased adversity severity was associated with decreased latent agency beliefs. These findings suggest that exposure to adversity in childhood has a nuanced impact on reinforcement learning and agency beliefs in adulthood.

Neurosciences & Neurology↗

Dark Matter Search Results from the Complete Exposure of the PICO-60 C$_3$F$_8$ Bubble Chamber

Final results are reported from operation of the PICO-60 C3F8 dark matter detector, a bubble chamber filled with 52 kg of C3F8 located in the SNOLAB underground laboratory. The chamber was operated at thermodynamic thresholds as low as 1.2 keV without loss of stability. A new blind 1404-kg-day exposure at 2.45 keV threshold was acquired with approximately the same expected total background rate as the previous 1167-kg-day exposure at 3.3 keV. This increased exposure is enabled in part by a new optical tracking analysis to better identify events near detector walls, permitting a larger fiducial volume. These results set the most stringent direct-detection constraint to date on the weakly interacting massive particle (WIMP)-proton spin-dependent cross section at 3.2×10-41 cm2 for a 25 GeV WIMP, improving on previous PICO results for 3–5 GeV WIMPs by an order of magnitude.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Band structure of Bi surfaces formed on Bi 2 Se 3 upon exposure to air

Bi 2 Se 3 has been the focus of intense interest over the past decade due to its topological properties. Bi surfaces are known to form on Bi 2 Se 3 upon exposure to atmosphere, but their electronic structure has not been investigated. Here, we report band structure measurements of such Bi surfaces using angle-resolved photoemission spectroscopy. Measured spectra can be well explained by the band structure of a single bilayer of Bi on Bi 2 Se 3 , and show that Bi surfaces consistently dominate the photoemission signal for air exposure times of at least 1 hour. These results demonstrate that atmospheric effects should be taken into consideration when identifying two-dimensional transport channels, and when designing surface-sensitive measurements of Bi 2 Se 3 , ideally limiting air exposure to no more than a few minutes.

36 MATERIALS SCIENCE↗

Grapevine Plasticity in Response to an Altered Microclimate: Sauvignon Blanc Modulates Specific Metabolites in Response to Increased Berry Exposure

In this paper, the metabolic and physiological impacts of an altered microclimate on quality-associated primary and secondary metabolites in grape (Vitis vinifera) ‘Sauvignon Blanc’ berries was determined in a high-altitude vineyard. The leaf and lateral shoot removal in the bunch zones altered the microclimate by increasing the exposure of the berries. The physical parameters (berry diameter and weight), primary metabolites (sugars and organic acids), as well as bunch temperature and leaf water potential were predominantly not affected by the treatment. The increased exposure led to higher levels of specific carotenoids and volatile terpenoids in the exposed berries, with earlier berry stages reacting distinctly from the later developmental stages. Plastic/nonplastic metabolite responses could be further classified to identify metabolites that were developmentally controlled and/or responded to the treatment in a predictable fashion (assessed over two consecutive vintages). The study demonstrates that grapevine berries exhibit a degree of plasticity within their secondary metabolites and respond physiologically to the increased exposure by increasing metabolites with potential antioxidant activity. Finally, taken together, the data provide evidence that the underlying physiological responses relate to the maintenance of stress pathways by modulating antioxidant molecules in the berries.

59 BASIC BIOLOGICAL SCIENCES↗

The Natural and Accelerated Evolution of EVA Adhesion Through Intermediate Exposures

Ethylene vinyl acetate (EVA) encapsulants comprise the majority of the encapsulants currently in use; much work has been done to understand and model the adhesive characteristics of EVA-encapsulated modules, but limited work has provided reliable insight into adhesion during the intermediate stages of exposure, limiting the ability to validate model predictions in this range. We provide the adhesion energy measurements for EVA adhesion after nearly six years of field aging and 10 000 h of accelerated aging. Both field and accelerated aging reveal a distinct plateau that emerges during the intermediate exposure periods (after one year in the field and after 1000 h in a chamber). At 10 000 h, adhesion within accelerated aged minimodules falls to a level generally seen after long-term field exposures (>15 years). Previous modeling predicted that adhesion would steadily decrease over the lifetime of a module, but these current results uncover an intermediate plateauing trend that is important to accurately modeling the evolution of adhesion and predicting adhesive failure. Based on these findings, three key model refinements concerning the rate of UV-radical formation and subsequent β-scission, the rate and acceleration of hydrolytic depolymerization, and the profile of the plasticity contribution over time are implemented and discussed.

14 SOLAR ENERGY↗

Road exposure and the detectability of birds in field surveys

Road ecology, the study of the impacts of roads and their traffic on wildlife, including birds, is a rapidly growing field, with research showing effects on local avian population densities up to several kilometres from a road. However, in most studies, the effects of roads on the detectability of birds by surveyors are not accounted for. This could be a significant source of error in estimates of the impacts of roads on birds and could also affect other studies of bird populations. Using road density, traffic volume and bird count data from across Great Britain, we assess the relationships between roads and detectability of a range of bird species. Of 51 species analysed, the detectability of 36 was significantly associated with road exposure, in most cases inversely. Across the range of road exposure recorded for each species, the mean positive change in detectability was 52% and the mean negative change was 36%, with the strongest negative associations found in smaller‐bodied species and those for which aural cues are more important in detection. These associations between road exposure and detectability could be caused by a reduction in surveyors’ abilities to hear birds or by changes in birds’ behaviour, making them harder or easier to detect. We suggest that future studies of the impacts of roads on populations of birds or other taxa, and other studies using survey data from road‐exposed areas, should account for the potential impacts of roads on detectability.

Cooke, Sophia C.↗

WE-G-204-02: Utility of a Channelized Hotelling Model Observer Over a Large Range of Angiographic Exposure Levels

Purpose: Mathematical model observers provide a figure of merit that simultaneously considers a test object and the contrast, noise, and spatial resolution properties of an imaging system. The purpose of this work was to investigate the utility of a channelized Hotelling model observer (CHO) to assess system performance over a large range of angiographic exposure conditions. Methods: A 4 mm diameter disk shaped, iodine contrast test object was placed on a 20 cm thick Lucite phantom and 1204 image frames were acquired using fixed x-ray beam quality and for several detector target dose (DTD) values in the range 6 to 240 nGy. The CHO was implemented in the spatial domain utilizing 96 Gabor functions as channels. Detectability index (DI) estimates were calculated using the “resubstitution” and “holdout” methods to train the CHO. Also, DI values calculated using discrete subsets of the data were used to estimate a minimally biased DI as might be expected from an infinitely large dataset. The relationship between DI, independently measured CNR, and changes in results expected assuming a quantum limited detector were assessed over the DTD range. Results: CNR measurements demonstrated that the angiography system is not quantum limited due to relatively increasing contamination from electronic noise that reduces CNR for low DTD. Direct comparison of DI versus CNR indicates that the CHO relatively overestimates DI for low DTD and/or underestimates DI values for high DTD. The relative magnitude of the apparent bias error in the DI values was ∼20% over the 40x DTD range investigated. Conclusion: For the angiography system investigated, the CHO can provide a minimally biased figure of merit if implemented over a restricted exposure range. However, bias leads to overestimates of DI for low exposures. This work emphasizes the need to verify CHO model performance during real-world application.

60 APPLIED LIFE SCIENCES↗