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At least 919 records · Page 51

Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts

Abstract Efficient conversion of pentose sugars remains a significant barrier to the replacement of petroleum-derived chemicals with plant biomass-derived bioproducts. While the oleaginous yeast Rhodosporidium toruloides (also known as Rhodotorula toruloides ) has a relatively robust native metabolism of pentose sugars compared to other wild yeasts, faster assimilation of those sugars will be required for industrial utilization of pentoses. To increase the rate of pentose assimilation in R. toruloides, we leveraged previously reported high-throughput fitness data to identify potential regulators of pentose catabolism. Two genes were selected for further investigation, a putative transcription factor (RTO4_12978, Pnt1) and a homolog of a glucose transceptor involved in carbon catabolite repression (RTO4_11990). Overexpression of Pnt1 increased the specific growth rate approximately twofold early in cultures on xylose and increased the maximum specific growth by 18% while decreasing accumulation of arabitol and xylitol in fast-growing cultures. Improved growth dynamics on xylose translated to a 120% increase in the overall rate of xylose conversion to fatty alcohols in batch culture. Proteomic analysis confirmed that Pnt1 is a major regulator of pentose catabolism in R. toruloides. Deletion of RTO4_11990 increased the growth rate on xylose, but did not relieve carbon catabolite repression in the presence of glucose. Carbon catabolite repression signaling networks remain poorly characterized in R. toruloides and likely comprise a different set of proteins than those mainly characterized in ascomycete fungi.

59 BASIC BIOLOGICAL SCIENCES↗

Integrated photonics on thin-film lithium niobate

Lithium niobate (LN), an outstanding and versatile material, has influenced our daily life for decades—from enabling high-speed optical communications that form the backbone of the Internet to realizing radio-frequency filtering used in our cell phones. This half-century-old material is currently embracing a revolution in thin-film LN integrated photonics. The successes of manufacturing wafer-scale, high-quality thin films of LN-on-insulator (LNOI) and breakthroughs in nanofabrication techniques have made high-performance integrated nanophotonic components possible. With rapid development in the past few years, some of these thin-film LN devices, such as optical modulators and nonlinear wavelength converters, have already outperformed their legacy counterparts realized in bulk LN crystals. Furthermore, the nanophotonic integration has enabled ultra-low-loss resonators in LN, which has unlocked many novel applications such as optical frequency combs and quantum transducers. In this review, we cover—from basic principles to the state of the art—the diverse aspects of integrated thin-film LN photonics, including the materials, basic passive components, and various active devices based on electro-optics, all-optical nonlinearities, and acousto-optics. We also identify challenges that this platform is currently facing and point out future opportunities. The field of integrated LNOI photonics is advancing rapidly and poised to make critical impacts on a broad range of applications in communication, signal processing, and quantum information.

Zhu, Di (ORCID:0000000302101860)↗

Measurements of angular distance and momentum ratio distributions in three-jet and $${\text {Z}}$$ + two-jet final states in $${\text {p}}{\text {p}}$$ collisions

Collinear (small-angle) and large-angle, as well as soft and hard radiations are investigated in three-jet and Z + two-jet events collected in proton-proton collisions at the LHC. The normalized production cross sections are measured as a function of the ratio of transverse momenta of two jets and their angular separation. The measurements in the three-jet and Z + two-jet events are based on data collected at a center-of-mass energy of 8TeV, corresponding to an integrated luminosity of 19.8fb -1 . The Z + two-jet events are reconstructed in the dimuon decay channel of the Z boson. The three-jet measurement is extended to include $\sqrt {s}$=13TeV data corresponding to an integrated luminosity of 2.3fb -1 . The results are compared to predictions from event generators that include parton showers, multiple parton interactions, and hadronization. The collinear and soft regions are in general well described by parton showers, whereas the regions of large angular separation are often best described by calculations using higher-order matrix elements.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Search for K + decays into the π + e + e - e + e - final state

The first search for ultra-rare K + decays into the π + e + e - e + e - final state is reported, using a dataset collected by the NA62 experiment at CERN in 2017–2018. An upper limit of 1.4 x 10 -8 at 90% CL is obtained for the branching ratio of the K + → π + e + e - e + e - decay, predicted in the Standard Model to be (7.2 ± 0.7) x 10 -11 . Upper limits at 90% CL are obtained at the level of 10 -9 for the branching ratios of two prompt decay chains involving pair-production of hidden-sector mediators: K + → π + aa,a → e + e - and K + → π + S,S → A'A',A' → e + e - .

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Measurement of the $K^{+} → π^{+} γγ$ decay

A sample of 3984 candidates of the $K^{+} → π^{+} γγ$ decay, with an estimated background of 291 ± 14 events, was collected by the NA62 experiment at CERN during 2017–2018. In order to describe the observed di-photon mass spectrum, the next-to-leading order contribution in chiral perturbation theory was found to be necessary. The decay branching ratio in the full kinematic range is measured to be (9.61 ± 0.17) x 10 -7 . The first search for production and prompt decay of an axion-like particle with gluon coupling in the process $K^{+} → π^{+} a$, $a → γγ$ is also reported.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Optimization and understanding of ZnO nanoarray supported Cu-ZnO-Al 2 O 3 catalyst for enhanced CO 2 -methanol conversion at low temperature and pressure

Cu-ZnO-Al 2 O 3 is the most widely applied catalyst for CO 2 hydrogenation to methanol. However, it is still a challenge to produce methanol using this catalyst under low-temperature (<250 °C) and low-pressure (<10 bar) conditions with desirable yield and selectivity. In this work, by tuning the experimental processing parameters such as solvent, loading amount, and annealing temperature, highly improved ZnO nanoarray supported Cu-ZnO-Al 2 O 3 catalysts have been successfully demonstrated. Here, by using organic solvent (N,N-dimethylformamide (DMF), acetone, or isopropanol) for dip-coating loading process instead of deionized (DI) water, Cu-ZnO-Al 2 O 3 nanocatalysts was comparatively better dispersed on the nanorod array support with populated and abundant active sites, thus enhancing the methanol yield. With the control of the loading amount and annealing temperature, finely distributed Cu nanoparticles were obtained on the ZnO nanorod surfaces to enhance the interactions between Cu and ZnO nanorod surfaces. Further improvement of the catalyst performance is demonstrated by tuning the reaction space velocity. At 200 °C and 10 bar conditions, the optimized catalyst achieved a methanol yield of 6.46 mol h –1 kg –1 with 100 % selectivity. The good stability after prolonged testing of the catalysts demonstrates the potential practical implementation. The in situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) measurements under the 1 bar reveal that the CO 2 hydrogenation to methanol on the ZnO nanoarray supported Cu-ZnO-Al 2 O 3 catalyst follows the CO reaction pathway, due to the surface oxygen vacancies on ZnO nanorods which facilitate CO 2 dissociation.

77 NANOSCIENCE AND NANOTECHNOLOGY↗

Shocks in the stacked Sunyaev-Zel’dovich profiles of clusters II: Measurements from SPT-SZ + Planck Compton- y map

ABSTRACT We search for the signature of cosmological shocks in stacked gas pressure profiles of galaxy clusters using data from the South Pole Telescope (SPT). Specifically, we stack the latest Compton-y maps from the 2500 deg2 SPT-SZ survey on the locations of clusters identified in that same data set. The sample contains 516 clusters with mean mass $\langle M_{\rm 200m}\rangle = 10^{14.9} \, {\rm M}_\odot$ and redshift 〈z〉 = 0.55. We analyse in parallel a set of zoom-in hydrodynamical simulations from the three hundred project. The SPT-SZ data show two features: (i) a pressure deficit at R/R200m = 1.08 ± 0.09, measured at 3.1σ significance and not observed in the simulations, and; (ii) a sharp decrease in pressure at R/R200m = 4.58 ± 1.24 at 2.0σ significance. The pressure deficit is qualitatively consistent with a shock-induced thermal non-equilibrium between electrons and ions, and the second feature is consistent with accretion shocks seen in previous studies. We split the cluster sample by redshift and mass, and find both features exist in all cases. There are also no significant differences in features along and across the cluster major axis, whose orientation roughly points towards filamentary structure. As a consistency test, we also analyse clusters from the Planck and Atacama Cosmology Telescope Polarimeter surveys and find quantitatively similar features in the pressure profiles. Finally, we compare the accretion shock radius ($R_{\rm sh,\, acc}$) with existing measurements of the splashback radius (Rsp) for SPT-SZ and constrain the lower limit of the ratio, $R_{\rm sh,\, acc}/R_{\rm sp}\gt 2.16 \pm 0.59$.

79 ASTRONOMY AND ASTROPHYSICS↗

Data-driven background model for the CUORE experiment

Here, we present the model we developed to reconstruct the CUORE radioactive background based on the analysis of an experimental exposure of 1038.4 kg yr. The data reconstruction relies on a simultaneous Bayesian fit applied to energy spectra over a broad energy range. The high granularity of the CUORE detector, together with the large exposure and extended stable operations, allow for an in-depth exploration of both spatial and time dependence of backgrounds. We achieve high sensitivity to both bulk and surface activities of the materials of the setup, detecting levels as low as 10 nBq kg −1 and 0.1 nBq cm −2 , respectively. We compare the contamination levels we extract from the background model with prior radio-assay data, which informs future background risk mitigation strategies. The results of this background model play a crucial role in constructing the background budget for the CUPID experiment as it will exploit the same CUORE infrastructure.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Optimization of a single module of CUPID

CUPID is the next generation experiment which will use scintillating cryogenic calorimeters to search for the neutrinoless double β decay. This unobserved process would shed light on the nature of the neutrino, which up to our knowledge could be a Majorana or a Dirac particle, and would give us an important hint to explain the lack of antimatter in the universe. This ambitious search needs a detector with unique characteristics such as an extremely low background level and an excellent energy resolution. CUPID is now in advanced R&D state to optimize the detector design in order to completely exploit the potentialities of scintillating cryogenic calorimeters. In the following I will describe the test performed at the LNGS (Laboratori Nazionali del Gran Sasso) of a single module of the future CUPID detector. In this contribution we present the performance obtained with a novel assembly concept, proving that it matches the requirements for CUPID.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

An energy-dependent electro-thermal response model of CUORE cryogenic calorimeter

The Cryogenic Underground Observatory for Rare Events (CUORE) is the most sensitive experiment searching for neutrinoless double-beta decay (0νββ) in 130 Te. CUORE uses a cryogenic array of 988 TeO 2 calorimeters operated at ~10 mK with a total mass of 741 kg. To further increase the sensitivity, the detector response must be well understood. Here, we present a non-linear thermal model for the CUORE experiment on a detector-by-detector basis. We have examined both equilibrium and dynamic electro-thermal models of detectors by numerically fitting non-linear differential equations to the detector data of a subset of CUORE channels which are well characterized and representative of all channels. Further, we demonstrate that the hot-electron effect and electric-field dependence of resistance in NTD-Ge thermistors alone are inadequate to describe our detectors' energy-dependent pulse shapes. We introduce an empirical second-order correction factor in the exponential temperature dependence of the thermistor, which produces excellent agreement with energy-dependent pulse shape data up to 6 MeV. We also present a noise analysis using the fitted thermal parameters and show that the intrinsic thermal noise is negligible compared to the observed noise for our detectors.

47 OTHER INSTRUMENTATION↗

New Direct Limit on Neutrinoless Double Beta Decay Half-Life of 128 $\mathrm{Te}$ with CUORE

The Cryogenic Underground Observatory for Rare Events (CUORE) at Laboratori Nazionali del Gran Sasso of INFN in Italy is an experiment searching for neutrinoless double beta (0νββ) decay. Its main goal is to investigate this decay in 130 Te, but its ton-scale mass and low background make CUORE sensitive to other rare processes as well. Here, in this Letter, we present our first results on the search for 0νββ decay of 128 Te, the Te isotope with the second highest natural isotopic abundance. We find no evidence for this decay, and using a Bayesian analysis we set a lower limit on the 128 Te 0νββ decay half-life of T 1/2 > 3.6 x 10 24 yr (90% CI). This represents the most stringent limit on the half-life of this isotope, improving by over a factor of 30 the previous direct search results, and exceeding those from geochemical experiments for the first time.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

The Short-Baseline Near Detector at Fermilab: Input to the European Strategy for Particle Physics 2026 Update

SBND is a 112 ton liquid argon time projection chamber (LArTPC) neutrino detector located 110 meters from the Booster Neutrino Beam (BNB) target at Fermilab. Its main goals include searches for eV-scale sterile neutrinos as part of the Short-Baseline Neutrino (SBN) program, other searches for physics beyond the Standard Model, and precision studies of neutrino-argon interactions. In addition, SBND is providing a platform for LArTPC neutrino detector technology development and is an excellent training ground for the international group of scientists and engineers working towards the upcoming flagship Deep Underground Neutrino Experiment (DUNE). SBND began operation in July 2024, and started collecting stable neutrino beam data in December 2024 with an unprecedented rate of ~7,000 neutrino events per day. During its currently approved operation plans (2024-2027), SBND is expected to accumulate nearly 10 million neutrino interactions. The near detector dataset will be instrumental in testing the sterile neutrino hypothesis with unprecedented sensitivity in SBN and in probing signals of beyond the Standard Model physics. It will also be used to significantly advance our understanding of the physics of neutrino-argon interactions ahead of DUNE. After the planned accelerator restart at Fermilab (2029+), opportunities are being explored to operate SBND in antineutrino mode in order to address the scarcity of antineutrino-argon scattering data, or in a dedicated beam-dump mode to significantly enhance sensitivity to searches for new physics. SBND is an international effort, with approximately 40% of institutions from Europe, contributing to detector construction, commissioning, software development, and data analysis. Continued European involvement and leadership are essential during SBND's operations and analysis phase for both the success of SBND, SBN and its role leading up to DUNE.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗