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At least 919 records · Page 51

Genetic dissection of natural variation in oilseed traits of camelina by whole‐genome resequencing and QTL mapping

Abstract Camelina [ Camelina sativa (L.) Crantz] is an oilseed crop in the Brassicaceae family that is currently being developed as a source of bioenergy and healthy fatty acids. To facilitate modern breeding efforts through marker‐assisted selection and biotechnology, we evaluated genetic variation among a worldwide collection of 222 camelina accessions. We performed whole‐genome resequencing to obtain single nucleotide polymorphism (SNP) markers and to analyze genomic diversity. We also conducted phenotypic field evaluations in two consecutive seasons for variations in key agronomic traits related to oilseed production such as seed size, oil content (OC), fatty acid composition, and flowering time. We determined the population structure of the camelina accessions using 161,301 SNPs. Further, we identified quantitative trait loci (QTL) and candidate genes controlling the above field‐evaluated traits by genome‐wide association studies (GWAS) complemented with linkage mapping using a recombinant inbred line (RIL) population. Characterization of the natural variation at the genome and phenotypic levels provides valuable resources to camelina genetic studies and crop improvement. The QTL and candidate genes should assist in breeding of advanced camelina varieties that can be integrated into the cropping systems for the production of high yield of oils of desired fatty acid composition.

59 BASIC BIOLOGICAL SCIENCES↗

A view of the pan‐genome of domesticated Cowpea ( Vigna unguiculata [L.] Walp.)

Abstract Cowpea, Vigna unguiculata L . Walp., is a diploid warm‐season legume of critical importance as both food and fodder in sub‐Saharan Africa. This species is also grown in Northern Africa, Europe, Latin America, North America, and East to Southeast Asia. To capture the genomic diversity of domesticates of this important legume, de novo genome assemblies were produced for representatives of six subpopulations of cultivated cowpea identified previously from genotyping of several hundred diverse accessions. In the most complete assembly (IT97K‐499‐35), 26,026 core and 4963 noncore genes were identified, with 35,436 pan genes when considering all seven accessions. GO terms associated with response to stress and defense response were highly enriched among the noncore genes, while core genes were enriched in terms related to transcription factor activity, and transport and metabolic processes. Over 5 million single nucleotide polymorphisms (SNPs) relative to each assembly and over 40 structural variants >1 Mb in size were identified by comparing genomes. Vu10 was the chromosome with the highest frequency of SNPs, and Vu04 had the most structural variants. Noncore genes harbor a larger proportion of potentially disruptive variants than core genes, including missense, stop gain, and frameshift mutations; this suggests that noncore genes substantially contribute to diversity within domesticated cowpea.

59 BASIC BIOLOGICAL SCIENCES↗

Encoding beyond cosmological horizons in de Sitter JT gravity

Black hole event horizons and cosmological event horizons share many properties, making it natural to ask whether our recent advances in understanding black holes generalize to cosmology. To this end, we discuss a paradox that occurs if observers can access what lies beyond their cosmological horizon in the same way that they can access what lies beyond a black hole horizon. In particular, distinct observers with distinct horizons may encode the same portion of spacetime, violating the no-cloning theorem of quantum mechanics. This paradox is due precisely to the observer-dependence of the cosmological horizon — the sharpest difference from a black hole horizon — although we will argue that the gravity path integral avoids the paradox in controlled examples.

2D gravity↗

Polarized fragmenting jet functions in inclusive and exclusive jet production

Abstract In this work, we present a complete theoretical framework for analyzing the distribution of polarized hadrons within jets, with and without measuring the transverse momentum relative to the standard jet axis. Using soft-collinear effective theory (SCET), we derive the factorization and provide the theoretical calculation of both semi-inclusive and exclusive fragmenting jet functions (FJFs) under longitudinal and transverse polarization. With the polarized FJFs, one gains access to a variety of new observables that can be used for extracting both collinear and transverse momentum dependent parton distribution functions (PDFs) and fragmentation functions (FFs). As examples, we provide numerical results for the spin asymmetry$$ {A}_{TU,T}^{\cos \left({\phi}_S-{\hat{\phi}}_{S_h}\right)} $$ A TU , T cos ϕ S − ϕ ̂ S h from polarized semi-inclusive hadron-in-jet production in polarizedppcollisions at RHIC kinematics, where a transversely polarized quark would lead to the transverse spin of the final-state hadron inside the jet and is thus sensitive to the transversity fragmentation functions. Similarly, another spin asymmetry,$$ {A}_{TU,L}^{\cos \left({\phi}_q-{\phi}_S\right)} $$ A TU , L cos ϕ q − ϕ S from polarized exclusive hadron-in-jet production in polarizedepcollisions at EIC kinematics would allow us to access the helicity fragmentation functions. These observables demonstrate promising potential in investigating transverse momentum dependent PDFs and FFs and are worthwhile for further measurements.

Physics↗

Simple holography in general spacetimes

The simple or “outermost” wedge in AdS is the portion of the entanglement wedge that can be reconstructed with sub-exponential effort from CFT data. Here we furnish a definition in arbitrary spacetimes: given an input wedge a analogous to a CFT boundary region, the simple wedge z(a) is the largest wedge accessible by a “zigzag,” a certain sequence of antinormal lightsheets. We show that z(a) is a throat, and that it is contained in every other throat. This implies that z(a) is unique; that it is contained in the generalized entanglement wedge; and that it reduces to the AdS prescription as a special case. The zigzag explicitly constructs a preferred Cauchy slice that renders the simple wedge accessible from a; thus it adds a novel structure even in AdS. So far, no spacelike construction is known to reproduce these results, even in time-symmetric settings. This may have implications for the modeling of holographic encoding by tensor networks.

AdS-CFT Correspondence↗

Settling of two-way momentum and energy coupled particles subject to Boussinesq and non-Boussinesq heating

This work establishes a procedure to accurately compute heat transfer between an Eulerian fluid and Lagrangian point-particles. Recent work has focused on accurately computing momentum transfer between fluid and particles. The coupling term for momentum involves the undisturbed fluid velocity at the particle location which is not directly accessible in the simulation. Analogously, in the context of thermal coupling, the undisturbed fluid temperature at the particle location is not directly accessible in simulations and must be estimated. In this paper, we develop a scheme to accurately estimate the undisturbed fluid temperature of a point-particle exchanging thermal energy with a surrounding fluid. Furthermore, the temperature disturbance is correlated with the enhanced temperature curvature in the vicinity of the particle and is formally valid in the low heating, low convection limit. We conduct extensive verification of the correction procedure for a settling particle subject to radiation. This setup allows the simultaneous testing of thermal and momentum corrections. By considering equations of drag and Nusselt number extended to finite Péclet and Boussinesq numbers, we establish a large range over which the correction procedure can be applied.

42 ENGINEERING↗

Control of deviatoric stress in the diamond anvil cell through thermal expansion mismatch stress in thin films

Elastic and plastic properties of materials and phase transitions at extreme conditions vary with both hydrostatic pressure and deviatoric stress. To generate and measure controlled deviatoric stress at pressures beyond those accessible with large volume differential and rotational presses and optical access for spectroscopy, experiments tested the combination of diamond anvil cell and thin film technology. Thin films of polycrystalline Cr-doped Al 2 O 3 ruby were prepared using pulsed laser deposition on single-crystal substrates of either Al 2 O 3 sapphire or yttria-stabilized cubic zirconia for contrasting initial film stress, and loaded in diamond anvil cells for confining stress. The piezospectroscopic response of the ruby films demonstrates consistently higher deviatoric stress in the film on zirconia relative to the film on the control sapphire, and an increase in deviatoric stress with applied load. Complementary synchrotron X-ray diffraction of the zirconia substrate confirmed that no pressure-induced phase transitions impacted the stress state of the ruby film, but differences in compressibility of film and substrate result in changes in film stress analogous to thermal expansion mismatch. Furthermore, this technique may be applied to evaluate elastic and plastic response of thin films of a variety of materials under extreme stress.

36 MATERIALS SCIENCE↗

Pseudomonas atagosis sp. nov., and Pseudomonas akappagea sp. nov., New Soil Bacteria Isolated from Samples on the Volcanic Island Izu Oshima, Tokyo

During the exploration of microbial natural resources, two strains of Pseudomonas, PS14 T and PS24 T , were isolated from samples taken from Izu Oshima, a volcanic island located 120 km southwest of central Tokyo. Phylogenetic analysis based on 16S rRNA gene sequences showed that PS14 T was most similar to Pseudomonas baetica a390T (99.6%) and Pseudomonas helmanticensis OHA11 T (99.5%), and that PS24T was most similar to Pseudomonas qingdaonensis JJ3 T (98.8%) and Pseudomonas lutea OK2 T (98.7%). The major fatty acids of these two strains were C 16:0 and C 17:0 cyclo, summed feature 3 (C 16:1 ω6c and/or C 16:1 ω7c), and summed feature 8 (C18:1 ω7c and/or 18:1 ω6c). The phylogenetic analyses, DNA-DNA hybridization results and phenotypic traits indicated that PS14 T and PS24 T constitute two novel species, Pseudomonas atagosis sp. nov. (type strain PS14 T = CECT 9940 T , = LMG 31496 T ) and Pseudomonas akappagea sp. nov. (type strain PS24T = CECT 9941T, = LMG 31497T), respectively. The sequence data of the draft genomes of PS14 T and PS24 T were deposited in the GenBank database under accession numbers VXCA00000000 and VXCP00000000, respectively, and the sequence data of their 16S rRNA genes were deposited in the GenBank database under accession numbers MN396717 and MN382268, respectively.

59 BASIC BIOLOGICAL SCIENCES↗

Interspecific variation in the timing and magnitude of hydraulic redistribution in a forest with distinct water sources

Trees regulate water availability among their rooting strata through a nocturnal, passive transference of water known as hydraulic redistribution (HR). This study investigates differences in HR and groundwater use among common canopy species in longleaf pine (Pinus palustris Mill., Pinaceae) woodlands and explores environmental factors influencing HR. HR was estimated by sap flux of lateral roots and main stems of three mature canopy species (P. palustris, Quercus laevis Walter., Fagaceae and Quercus margarettae Ashe., Fagaceae). We used δ18O and δD of xylem water, soil water, and groundwater to determine water source. Finally, we related HR to environmental factors (Temperature, VWC, VPD) to better understand controls of HR dynamics. Pinus palustris had higher water use than either Quercus species, and also redistributed significantly more water as a nocturnal subsidy. HR fluxes were inversely related with mean nightly temperature and independent of shallow soil moisture. Stable isotope mixing models, based on δ 18 O and δD, indicated that all species have access to groundwater, but utilized shallow soil water in differing amounts when available. In systems with strong water potential gradients among soil strata, any species with access to a groundwater source is likely capable of HR; however, the magnitude of HR varies significantly by species, even among closely related taxa.

54 ENVIRONMENTAL SCIENCES↗

Interaction between the emerging components of online shopping and in-person activities: insights from a behavioral survey

The rise of technological advancements has led to the commonplace practice of online shopping for retail, grocery, and food. However, little research has been conducted on the interplay of these components in burdened communities (BCs) that face issues of marginalization and limited access to digital resources. Here, this study aims to provide a comprehensive understanding of travel behavior changes by analyzing the interconnectedness of the emerging components of online shopping (retail, grocery, and food) and in-person activities in both BCs and non-BCs. A unique household-level database is created by linking the 2021 Puget Sound Household Travel Survey and the US Department of Transportation’s burdened community databases, and a conditional mixed process model is estimated to account for unobserved endogeneity. The findings suggest households living in BCs are less likely to order online retail goods and groceries compared to non-BC households. Additionally, the probability of making more restaurant trips decreases for households living in BCs. The study highlights the digital divide that exists in BCs and the differences in online and in-person shopping activities across socioeconomic levels. Policymakers may address these disparities to promote better access to goods and services for all. Besides, planners may need to improve the travel demand models by accounting for the emerging components of online shopping and the trip frequencies by purpose in BCs.

Digital Divide↗

Pathways to commercial building plug and process load efficiency and control

Abstract To accomplish net-zero carbon emissions in the built environment by 2050, we must equitably decarbonize commercial buildings, including reducing plug and process loads (PPLs). PPLs are plug-in or hardwired electric and gas loads that are not associated with major building end uses like lighting and HVAC. Research shows PPL energy reduction strategies and control technologies have the potential to save energy. But even when implemented, these savings have rarely been achieved and there has not been widespread uptake in U.S. commercial buildings. We investigate why these technologies and strategies have not seen widespread adoption and identify behavior and technology pathways to increase PPL reduction in U.S. commercial buildings. We examined behaviors of commercial building stakeholders through 44 interviews and cross-referenced qualitative analysis findings with in-depth technical knowledge of existing PPL control technologies and reduction strategies. PPL control implementation must be paired with management strategies, such as occupant engagement and training, to achieve optimal savings, and best practices should be disseminated across the industry. We found that increasing access to cost and energy savings data will promote uptake of PPL control technologies and allow designers to better incorporate PPLs into building design. Improving access to funding for PPL energy efficiency projects and addressing the split-incentive problem will increase adoption of PPL efficiency and control. Code bodies should continue to include PPL monitoring and reduction measures in energy codes. Key building stakeholders, including cybersecurity and information technology teams, should be involved in PPL monitoring and reduction strategy processes for successful implementation.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Understanding the Effects of Ash Content on Various Pretreatment Technologies for the Bioconversion of Corn Stover

Here, the study aimed to examine the effects of adding biomass ash on the biochemical processes involved in fermentable sugar production. Corn stover was pretreated using several methods—hot water, dilute acid, alkaline, γ-valerolactone, and ionic liquid methods, each examined with ash loadings of 7.18% and 21.07%. The findings demonstrated that increased ash content adversely affected both pretreatment and enzymatic hydrolysis. Specifically, the total sugar yield was 3 to 16% lower at the higher ash content across all pretreatment methods, and up to 4.01% lower during enzymatic hydrolysis. For acidic pretreatment, the sugar yield decreased as ash content increased. In contrast, ash content had a lesser impact on alkaline pretreatment compared to acidic pretreatment. For example, using corn stover with an ash content as high as 22.65% resulted in only a 2.90% decrease in total sugar yield compared to corn stover without added ash. Further, the primary reasons for the reduced sugar yield in higher ash biomass during acidic pretreatments were likely the neutralizing effect of the ash and decreased acid access to the substrates. During enzymatic hydrolysis, ash reduced the sugar yield by limiting enzyme access to cellulose.

09 BIOMASS FUELS↗

Prioritizing vaccination based on analysis of community networks

Abstract Many countries that had early access to COVID-19 vaccines implemented vaccination strategies that prioritized health care workers and the elderly. As barriers to access eased, vaccine prioritization strategies have been relaxed. However, these strategies are still an important tool for decision makers to manage new variants, plan for future booster shots, or stage mass vaccinations. This paper explores the impact of vaccine prioritization strategies using networks that represent communities with different demographics and connectivity. The impact of vaccination is compared to non-medical intervention to reduce transmission. Several sources of uncertainty are considered, including vaccine willingness and mask effectiveness. This paper finds that while prioritization strategies can have a large impact on reducing deaths and peak hospitalization, selecting the best strategy depends on community characteristics and the desired objective. Additionally, in some cases random vaccination performs as well as more targeted prioritization strategies. Understanding these trade-offs is important when planning vaccine distribution.

Klise, Katherine↗

Performance of Julia for High Energy Physics Analyses

We argue that the Julia programming language is a compelling alternative to currently more common implementations in Python and C++ for common data analysis workflows in high energy physics. We compare the speed of implementations of different workflows in Julia with those in Python and C++. Furthermore, our studies show that the Julia implementations are competitive for tasks that are dominated by computational load rather than data access. For work that is dominated by data access, we demonstrate an application with concurrent file reading and parallel data processing.

97 MATHEMATICS AND COMPUTING↗

Endogeneity of pedestrian survival time and emergency medical service response time: Variations across disadvantaged and non-disadvantaged communities

The Vision Zero-Safe Systems Approach prioritizes fast access to Emergency Medical Services (EMS) to improve the survivability of road users in transportation crashes, especially concerning the recent increase in pedestrian-involved crashes. Pedestrian crashes resulting in immediate or early death are considerably more severe than those taking longer. The time gap between injury and fatality is known as survival time, and it heavily relies on EMS response time. The characteristics of the crash location may be associated with EMS response and survival time. A US Department of Transportation initiative identifies communities often facing challenges. Six disadvantaged community (DAC) indicators, including economy, environment, equity, health, resilience, and transportation access, enable an analysis of how survival and EMS response times vary across DACs and non-DACs. To this end, this study created a unique and comprehensive database by linking DACs data with 2017–2021 pedestrian-involved fatal crashes. This study utilizes two-stage residual inclusion models with segmentation for DACs and non-DACs accounting for the endogenous relationship between EMS response and pedestrian survival time. The results indicate that EMS response time is higher and pedestrian survival time is lower in DACs than in non-DACs. A delayed EMS response time is associated with a greater reduction in survival time in DACs compared to non-DACs. Factors, e.g., nighttime and interstate crashes, contribute to higher EMS response time, while pedestrian drugs, driver speeding, and hit-and-run behaviors are associated with a greater reduction in survival time in DACs than non-DACs. Finally, the implications of the findings are discussed in the paper.

60 APPLIED LIFE SCIENCES↗

Hybrid machine learning/physics-based approach for predicting oxide glass-forming ability

Predicting the liquid compositions that will vitrify at experimentally accessible quench rates remains one of the grand challenges in the field of condensed matter physics. This glass-forming ability can be quantified as the critical quench rate needed to suppress crystallization. Knowledge of this critical quench rate also informs which glass composition could be used for new applications. There have been several physical and empirical models presented in the literature to predict the critical quench rate/glass forming ability. These models range from those theoretically derived to those quantified only through experimental characterization. In this work, we instead propose a new method to calculate the critical quench rate using the recently developed toy landscape model combined with machine learning. The toy landscape model accesses the underlying physics that control the vitrification behavior by directly simulating the liquid thermodynamics and kinetics. In conclusion, the results are discussed in terms of industrial impact, physical insights, and how the glass science community can develop improved predictions of glass-forming ability.

Crystallization↗

A review of machine learning in building load prediction

The surge of machine learning in recent years has been empowering engineer modeling in various fields. The decreasing hardware cost, increasing data accessibility, and advances of building automation system (BAS) allow the collection and storage of a significant amount of building operation data. The two facts provide great opportunities of applying machine learning to building energy systems modeling and analysis. There are a great number of research papers on this topic but there lacks a comprehensive and general review to summarize the current development, limitations, gaps and future trend. In this review paper series, machine learning techniques in building energy system modeling and analysis are reviewed under the organization and logic of the machine learning definition by Tom M. Mitchell: a computer program is said to learn from experience E with respect to some class of tasks T and performance measure P if its performance at tasks in T, as measured by P, improves with experience E. This paper is the first part of the review paper series, which focuses on building load prediction. First, the applications of building load prediction model (task T) are reviewed. Then, the modeling algorithms improving machine learning performance and accuracy (performance P) are reviewed. At the same time, the literature on the data perspective for modeling (experience E), including data engineering from sensors level to data level, pre-processing, feature extraction and selection, is reviewed. Finally, what is well-studied and what is lacking but with great potential are concluded; the gaps between present and future utilization of machine learning techniques are identified; the future trend and development are also predicted. The target readers of this paper are not only researchers from the building side who can get exposed to cutting edge machine learning tools, but also those from machine learning side who can understand the potential and challenge to apply machine learning in buildings.

Liang, Zhang↗

What you get is not always what you see—pitfalls in solar array assessment using overhead imagery

Effective integration planning for small, distributed solar photovoltaic (PV) arrays into electric power grids requires access to high quality data: the location and power capacity of individual solar PV arrays. Unfortunately, national databases of small-scale solar PV do not exist; those that do are limited in their spatial resolution, typically aggregated up to state or national levels. While several promising approaches for solar PV detection have been published, strategies for evaluating the performance of these models are often highly heterogeneous from study to study. The resulting comparison of these methods for practical applications for energy assessments becomes challenging and may imply that the reported performance evaluations overly optimistic. The heterogeneity comes in many forms, each of which we explore in this work: the degree of diversity of the locations and sensors (e.g. different satellites, aerial photography) from which the training and validation data originate, the validation of ground truth (manual annotation of imagery vs known solar PV locations), the level of spatial aggregation (e.g. array-level vs regional estimates), and inconsistencies in the training and validation datasets (e.g. different datasets are used for each study and those data are not always made accessible). For each, we discuss emerging practices from the literature to address them or suggest directions of future research. As part of our investigation, we evaluate solar PV identification performance in two large regions: the entire state of Connecticut and the city of San Diego, CA. In Connecticut, we also use 33,114 known parcel-level solar PV installations from Berkeley Lab’s Tracking the Sun dataset to evaluate parcel-level performance and evaluate capacity estimates using 169 municipalities. We also make our code (which we call SolarMapper), pre-trained models, training data, and predictions publicly available and provide a web portal for interactively inspecting each prediction that was made. Here our findings suggest that traditional performance evaluation of the automated identification of solar PV from satellite imagery may be optimistic due to common limitations in the validation process. The takeaways from this work are intended to inform and catalyze the large-scale practical application of automated solar PV assessment techniques by energy researchers and professionals.

14 SOLAR ENERGY↗