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At least 919 records · Page 51

Calculations of hydrogen diffusivity in Zr-based alloys: Influence of alloying elements and effect of stress

This report summarizes the progress on modeling hydrogen diffusivity in Zr-based alloys. The presence of hydrogen (H) can detrimentally affect the mechanical properties of many metals and alloys. To mitigate these detrimental effects requires fundamental understanding of the thermodynamics and kinetics governing H pickup and hydride formation. In this work, we focus on H diffusion in Zr-based alloys by studying the effects of alloying elements and stress, factors that have been shown to strongly affect H pickup and hydride formation in nuclear fuel claddings. A recently developed accelerated kinetic Monte Carlo method is used for the study. It is found that for the alloys considered here, H diffusivity depends weakly on composition, with negligible effect at high temperatures in the range of 600-1200 K. Therefore, the small variation in compositions of these alloys is likely not a major cause of the very different H pickup rates. In contrast, stress strongly affects H diffusivity. This effect needs to be considered for studying hydride formation and delayed hydride cracking.

36 MATERIALS SCIENCE↗

High‐throughput profiling and analysis of plant responses over time to abiotic stress

Abstract Sorghum ( Sorghum bicolor (L.) Moench) is a rapidly growing, high‐biomass crop prized for abiotic stress tolerance. However, measuring genotype‐by‐environment (G x E) interactions remains a progress bottleneck. We subjected a panel of 30 genetically diverse sorghum genotypes to a spectrum of nitrogen deprivation and measured responses using high‐throughput phenotyping technology followed by ionomic profiling. Responses were quantified using shape (16 measurable outputs), color (hue and intensity), and ionome (18 elements). We measured the speed at which specific genotypes respond to environmental conditions, in terms of both biomass and color changes, and identified individual genotypes that perform most favorably. With this analysis, we present a novel approach to quantifying color‐based stress indicators over time. Additionally, ionomic profiling was conducted as an independent, low‐cost, and high‐throughput option for characterizing G x E, identifying the elements most affected by either genotype or treatment and suggesting signaling that occurs in response to the environment. This entire dataset and associated scripts are made available through an open‐access, user‐friendly, web‐based interface. In summary, this work provides analysis tools for visualizing and quantifying plant abiotic stress responses over time. These methods can be deployed as a time‐efficient method of dissecting the genetic mechanisms used by sorghum to respond to the environment to accelerate crop improvement.

59 BASIC BIOLOGICAL SCIENCES↗

Evaluation of Applied Stress on Atmospheric Corrosion and Pitting Characteristics in 304L Stainless Steel

For this study, the effects of applied stress, ranging from tensile to compressive, on the atmospheric pitting corrosion behavior of 304L stainless steel (SS304L) were analyzed through accelerated atmospheric laboratory exposures and microelectrochemical cell analysis. After exposing the lateral surface of a SS304L four-point bend specimen to artificial seawater at 50°C and 35% relative humidity for 50 d, pitting characteristics were determined using optical profilometry and scanning electron microscopy. The SS304L microstructure was analyzed using electron backscatter diffraction. Additionally, localized electrochemical measurements were performed on a similar, unexposed, SS304L four-point bend bar to determine the effects of applied stress on corrosion susceptibility. Under the applied loads and the environment tested, the observed pitting characteristics showed no correlation with the applied stress (from 250 MPa to -250 MPa). Pitting depth, surface area, roundness, and distribution were found to be independent of location on the sample or applied stress. The lack of correlation between pitting statistics and applied stress was more likely due to the aggressive exposure environment, with a sea salt loading of 4 g/m 2 chloride. The pitting characteristics observed were instead governed by the available cathode current and salt distribution, which are a function of sea salt loading, as well as pre-existing underlying microstructure. In microelectrochemical cell experiments performed in Cl - environments comparable to the atmospheric exposure and in environments containing orders of magnitude lower Cl - concentrations, effects of the applied stress on corrosion susceptibility were only apparent in open-circuit potential in low Cl - concentration solutions. Cl - concentration governed the current density and transpassive dissolution potential.

36 MATERIALS SCIENCE↗

Instantaneous velocity profile measurements in a turbulent boundary layer

Instantaneous wall shear stress and streamwise velocities have been measured simultaneously in a flat-plate, turbulent boundary layer at moderate Reynolds number in an effort to provide experimental support for large eddy simulations. Data were obtained using a buried-wire, wall shear gage and a hot-wire rake positioned in the log region of the flow. Fluctuations of the instantaneous U(+) versus Y(+) profiles about a mean law of the wall are shown to be significant and complex. Peak cross-correlation values between wall shear stress and the velocities are high, and reflect the passage of a large structure inclined at a small angle to the wall. Estimates of this angle are consistent with those made by other investigators. Conditional sampling techniques were used to detect the passage of various sizes and types of flow disturbances (events), and to estimate their mean frequency of occurrence. Events characterized by large aand sudden streamwise accelerations were found to be highly coherent throughout the log region and were strongly correlated with large fluctuations in wall shear stress. Phase randomness between the near-wall quantities and the outer velocities was small. The results suggest that the flow events detected by conditional sampling applied to velocities in the log region may be related to the bursting process.

Robinson, S. K.↗

Strength anisotropy of granular material consisting of perfectly round particles

Abstract The strength anisotropy of granular materials deposited under gravity has mostly been attributed to elongated particles' tendency to align long axes along the bedding plane direction. However, recent experiments on near‐spherical glass beads, for which preferred particle alignment is inapplicable, have exhibited surprisingly strong strength anisotropy. This study tests the hypothesis that certain amount of fabric anisotropy caused by the anisotropic stress during deposition under gravity can be locked in a circular‐particle deposit. Such locked‐in fabric anisotropy can withstand isotropic consolidation and leads to significant strength anisotropy. 2D discrete element method simulations of direct shear tests on circular‐particle deposits are conducted in this study, allowing for the monitoring of both stress and fabric. Simulations on both monodispersed and polydispersed circular‐particle samples generated under downward gravitational acceleration exhibit clear anisotropy in shear strength, thereby proving the hypothesis. When using contact normal‐based and void‐based fabric tensors to quantify fabric anisotropy in the material, we find that the intensity of anisotropy is discernible but low prior to shearing and is dependent on the consolidation process and the dispersity of the sample. The fact that samples with very low anisotropy intensity measurements still exhibit fairly strong strength anisotropy suggests that current typical contact normal‐based and void‐based second‐order fabric tensor formulations may not be very effective in reflecting the anisotropic peak shear strength of granular materials. Copyright © 2017 John Wiley & Sons, Ltd.

Wang, Rui↗

l-asparaginase induces intrinsic mitochondrial-mediated apoptosis in human gastric adenocarcinoma cells and impedes tumor progression

Highlights: • We first confirmed that l-asparagine is essential for the growth and proliferation of AGS cells. • We further clarified that l-asparagine deprivation by l-asparaginase promotes anti-proliferation and apoptosis of AGS cells. • We demonstrated that action of l-asparaginase alters mitochondrial membrane permeability and activates intrinsic apoptosis. • We also showed that l-asparaginase impedes tumor progression. l-asparagine essentially regulates growth and proliferation of cancer cells. l-asparaginase is an anti-cancer enzyme that deprives the cancer cells of l-asparagine. The purpose of this study was to explore the mechanism of a novel l-asparaginase from Pseudomonas fluorescens on l-asparagine deprivation mediated anti-proliferation, apoptosis in human gastric adenocarcinoma cells and to evaluate inhibition of angiogenesis. We observed that, the presence of extracellular l-asparagine was essential for the growth of AGS cells. l-asparagine deprivation by l-asparaginase induced metabolic stress, cytotoxicity and apoptosis by G0 phase cell-cycle arrest, modulated the mitochondrial membrane integrity, accelerated caspase-3 activation and instigated DNA damage. The RT-PCR analysis of pro-apoptosis genes: bak1, bax, bbc3, bik, pmaip1, bnip3l, apaf1, casp3, casp7 and casp9 were significantly higher (P xiap, bid, mcl1, and death receptor genes tnf and tradd were significantly down-regulated (P l-asparaginase alters mitochondrial membrane permeability and auxiliary activates intrinsic apoptosis. Therefore, this mechanistic approach might be considered as a targeted enzymotherapy against gastric adenocarcinoma.

60 APPLIED LIFE SCIENCES↗

Towards linking lab and field lifetimes of perovskite solar cells

Metal halide perovskite solar cells (PSCs) represent a promising low-cost, thin-film photovoltaic (PV) technology, with unprecedented power conversion efficiencies (PCEs) obtained for both single-junction and tandem applications. To push PSCs toward commercialization, it is critical, albeit challenging, to understand device reliability under real-world outdoor conditions where multiple stress factors (e.g., light, heat, humidity) coexist, generating complicated degradation behaviors. It is necessary to identify accelerated indoor testing protocols - which can correlate specific stressors with observed degradation modes in fielded devices - to quickly guide PSC development. Here, we use a state-of-the-art p-i-n PSC stack (with PCE up to ~25.5%) to show that indoor accelerated stability tests can predict our 6-month outdoor aging tests. Device degradation rates under illumination and at elevated temperatures are most instructive for understanding outdoor device reliability. Further, we also find that the indium tin oxide (ITO)/self-assembled monolayer (SAM)-based hole transport layer (HTL)/perovskite interface most strongly affects our device operation stability. Improving the ion-blocking properties of the SAM HTL increases averaged device operational stability at 50 degree C-85 °C by a factor of ~2.8, reaching over 1000 h at 85 °C and to near 8200 h at 50 °C with a projected 20% degradation, which is among the best to date for high-efficiency p-i-n PSCs.

14 SOLAR ENERGY↗

Viscoelastic behavior and life-time predictions

Fiber reinforced plastics were considered for many structural applications in automotive, aerospace and other industries. A major concern was and remains the failure modes associated with the polymer matrix which serves to bind the fibers together and transfer the load through connections, from fiber to fiber and ply to ply. An accelerated characterization procedure for prediction of delayed failures was developed. This method utilizes time-temperature-stress-moisture superposition principles in conjunction with laminated plate theory. Because failures are inherently nonlinear, the testing and analytic modeling for both moduli and strength is based upon nonlinear viscoelastic concepts.

Dillard, D. A.↗

Structural Benchmark Testing for Stirling Convertor Heater Heads

The National Aeronautics and Space Administration (NASA) has identified high efficiency Stirling technology for potential use on long duration Space Science missions such as Mars rovers, deep space missions, and lunar applications. For the long life times required, a structurally significant design limit for the Stirling convertor heater head is creep deformation induced even under relatively low stress levels at high material temperatures. Conventional investigations of creep behavior adequately rely on experimental results from uniaxial creep specimens, and much creep data is available for the proposed Inconel-718 (IN-718) and MarM-247 nickel-based superalloy materials of construction. However, very little experimental creep information is available that directly applies to the atypical thin walls, the specific microstructures, and the low stress levels. In addition, the geometry and loading conditions apply multiaxial stress states on the heater head components, far from the conditions of uniaxial testing. For these reasons, experimental benchmark testing is underway to aid in accurately assessing the durability of Stirling heater heads. The investigation supplements uniaxial creep testing with pneumatic testing of heater head test articles at elevated temperatures and with stress levels ranging from one to seven times design stresses. This paper presents experimental methods, results, post-test microstructural analyses, and conclusions for both accelerated and non-accelerated tests. The Stirling projects use the results to calibrate deterministic and probabilistic analytical creep models of the heater heads to predict their life times.

Krause, David L.↗

Failure Modes in Capacitors When Tested Under a Time-Varying Stress

Power-on failure has been the prevalent failure mechanism for solid tantalum capacitors in decoupling applications. A surge step stress test (SSST) has been previously applied to identify the critical stress level of a capacitor batch to give some predictability to the power-on failure mechanism [1]. But SSST can also be viewed as an electrically destructive test under a time-varying stress (voltage). It consists of rapidly charging the capacitor with incremental voltage increases, through a low resistance in series, until the capacitor under test is electrically shorted. When the reliability of capacitors is evaluated, a highly accelerated life test (HALT) is usually adopted since it is a time-efficient method of determining the failure mechanism; however, a destructive test under a time-varying stress such as SSST is even more time efficient. It usually takes days or weeks to complete a HALT test, but it only takes minutes for a time-varying stress test to produce failures. The advantage of incorporating a specific time-varying stress profile into a statistical model is significant in providing an alternative life test method for quickly revealing the failure mechanism in capacitors. In this paper, a time-varying stress that mimics a typical SSST has been incorporated into the Weibull model to characterize the failure mechanism in different types of capacitors. The SSST circuit and transient conditions for correctly surge testing capacitors are discussed. Finally, the SSST was applied for testing Ta capacitors, polymer aluminum capacitors (PA capacitors), and multi-layer ceramic (MLC) capacitors with both precious metal electrodes (PME) and base metal electrodes (BME). The test results are found to be directly associated with the dielectric layer breakdown in Ta and PA capacitors and are independent of the capacitor values, the way the capacitors were built, and the capacitors manufacturers. The test results also show that MLC capacitors exhibit surge breakdown voltages much higher than the rated voltage and that the breakdown field is inversely proportional to the dielectric layer thickness. The SSST data can also be used to comparatively evaluate the voltage robustness of capacitors for decoupling applications.

Liu, David (Donhang)↗

Effect of elevation, season and accelerated snowmelt on biogeochemical processes during isolated conifer needle litter decomposition

Increased drought and temperatures associated with climate change have implications for ecosystem stress with risk for enhanced carbon release in sensitive biomes. Litter decomposition is a key component of biogeochemical cycling in terrestrial ecosystems, but questions remain regarding the local response of decomposition processes to climate change. This is particularly complex in mountain ecosystems where the variable nature of the slope, aspect, soil type, and snowmelt dynamics play a role. Hence, the goal of this study was to determine the role of elevation, soil type, seasonal shifts in soil moisture, and snowmelt timing on litter decomposition processes. Experimental plots containing replicate deployments of harvested lodgepole and spruce needle litter alongside needle-free controls were established in open meadows at three elevations ranging from 2,800–3,500 m in Crested Butte, Colorado. Soil biogeochemistry variables including gas flux, porewater chemistry, and microbial ecology were monitored over three climatically variable years that shifted from high monsoon rains to drought. Results indicated that elevation and soil type influenced baseline soil biogeochemical indicators; however, needle mass loss and chemical composition were consistent across the 700 m elevation gradient. Rates of gas flux were analogously consistent across a 300 m elevation gradient. The additional variable of early snowmelt by 2–3 weeks had little impact on needle chemistry, microbial composition and gas flux; however, it did result in increased dissolved organic carbon in lodgepole porewater collections suggesting a potential for aqueous export. In contrast to elevation, needle presence and seasonal variability of soil moisture and temperature both played significant roles in soil carbon fluxes. Furthermore, during a pronounced period of lower moisture and higher temperatures, bacterial community diversity increased across elevation with new members supplanting more dominant taxa. Microbial ecological resilience was demonstrated with a return to pre-drought structure and abundance after snowmelt rewetting the following year. These results show similar decomposition processes across a 700 m elevation gradient and reveal the sensitivity but resilience of soil microbial ecology to low moisture conditions.

59 BASIC BIOLOGICAL SCIENCES↗

Two-dimensional concentrated-stress low-frequency piezoelectric vibration energy harvesters

Vibration-based energy harvesters using piezoelectric materials have long made use of the cantilever beam structure. Surmounting the deficiencies in one-dimensional cantilever-based energy harvesters has been a major focus in the literature. In this work, we demonstrate a strategy of using two-dimensional beam shapes to harvest energy from low frequency excitations. A characteristic Zigzag-shaped beam is created to compare against the two proposed two-dimensional beam shapes, all of which occupy a 25.4 × 25.4 mm{sup 2} area. In addition to maintaining the low-resonance bending frequency, the proposed beam shapes are designed with the goal of realizing a concentrated stress structure, whereby stress in the beam is concentrated in a single area where a piezoelectric layer may be placed, rather than being distributed throughout the beam. It is shown analytically, numerically, and experimentally that one of the proposed harvesters is able to provide significant increase in power production, when the base acceleration is set equal to 0.1 g, with only a minimal change in the resonant frequency compared to the current state-of-the-art Zigzag shape. This is accomplished by eliminating torsional effects, producing a more pure bending motion that is necessary for high electromechanical coupling. In addition, the proposed harvesters have a large effective beam tip whereby large tip mass may be placed while retaining a low-profile, resulting in a low volume harvester and subsequently large power density.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Irradiation-Accelerated Corrosion of Reactor Core Materials. Final Report

This project aims to understand how radiation accelerates corrosion of reactor core materials. The combination of high temperature, chemically aggressive coolants, a high radiation flux and mechanical stress poses a major challenge for the life extension of current light water reactors, as well as the success of most all GenIV concepts. Of these four drivers, the combination of radiation and corrosion places the most severe demands on materials, for which an understanding of the fundamental science is simply absent. Only a few experiments have been conducted to understand how corrosion occurs under irradiation, yet the limited data indicates that the effect is large; irradiation causes order of magnitude increases in corrosion rates. Without a firm understanding of the mechanisms by which radiation and corrosion interact in film formation, growth, breakdown and repair, the extension of the current LWR fleet beyond 60 years and the success of advanced nuclear energy systems are questionable. The proposed work will address the process of irradiation-accelerated corrosion that is important to all current and advanced reactor designs, but remains very poorly understood. An improved understanding of the role of irradiation in the corrosion process will provide the community with the tools to develop predictive models for in-reactor corrosion, and to address specific, important forms of corrosion such as irradiation assisted stress corrosion cracking.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Accelerated Aging Experiments for Capacitor Health Monitoring and Prognostics

This paper discusses experimental setups for health monitoring and prognostics of electrolytic capacitors under nominal operation and accelerated aging conditions. Electrolytic capacitors have higher failure rates than other components in electronic systems like power drives, power converters etc. Our current work focuses on developing first-principles-based degradation models for electrolytic capacitors under varying electrical and thermal stress conditions. Prognostics and health management for electronic systems aims to predict the onset of faults, study causes for system degradation, and accurately compute remaining useful life. Accelerated life test methods are often used in prognostics research as a way to model multiple causes and assess the effects of the degradation process through time. It also allows for the identification and study of different failure mechanisms and their relationships under different operating conditions. Experiments are designed for aging of the capacitors such that the degradation pattern induced by the aging can be monitored and analyzed. Experimental setups and data collection methods are presented to demonstrate this approach.

Prognostics of Electronics↗

Understanding thermal and mechanical effects on lithium plating in lithium-ion batteries

Lithium plating is one of the main concerns for cell durability, as cyclable lithium and cell capacity are decreased during the process. Thermal and mechanical loads can influence cell behavior, including lithium plating driving forces, as suggested by the Arrhenius equation and the Bruggeman relation, respectively. Hence, a 1D and a 3D model are employed to study the thermal and mechanical effects on lithium plating in a lithium-ion battery with a high areal capacity (4 mAh/cm 2 ). The 1D model assumes uniform mechanical and temperature profile, while the 3D model is capable of capturing the nonuniformity of stress and temperature in the cell. Further, the effects of mechanical deformation and different temperatures on the process of lithium plating are investigated in this study. With the help of the 1D charging simulations, it is observed that lower temperatures increase the risk of lithium plating and the presence of mechanical deformations can accelerate its initiation. Furthermore, 3D charging simulations indicated that folds and boundaries of jellyrolls are more susceptible to lithium plating due to the higher stress concentration and lower temperature, respectively.

25 ENERGY STORAGE↗

An experimental search for near-wall boundary conditions for large eddy simulation

Instantaneous wall shear stress and streamwise velocities have been measured simultaneously in a flat plate, turbulent boundary layer at moderate Reynolds number in an effort to provide experimental support for large eddy simulations. Data were obtained by using a buried-wire wall shear gage and a hot-wire rake positioned in the log region of the flow. All data processing was accomplished with digital data analysis techniques on a minicomputer. Fluctuations of the instantaneous U plus versus Y plus profiles about a mean law of the wall are shown to be significant and complex. Peak cross-correlation values between wall shear stress and the velocities are high and reflect the passage of a large structure inclined at a small angle to the wall. Estimates of this angle are consistent with those made by other investigators. Conditional sampling techniques were used to detect the passage of various sizes and types of flow disturbances (events) and to estimate their mean frequency of occurrence. Events characterized by large and sudden streamwise accelerations were found to be highly coherent throughout the log region and were strongly correlated with large fluctuations in wall shear-stress. Phase randomness between the near-wall quantities and the outer velocities was small. The results suggest that the flow events detected by conditional sampling applied to velocities in the log region may be related to the bursting process.

Robinson, S. K.↗

What is needed for BISCO to work in a dipole insert for 20 tesla hybrid accelerator magnets

Developing HTS dipole inserts producing fields larger than 5 T within 15 T Nb3Sn outserts is necessary to generate 20 T or higher fields for future high energy colliders. Dipole inserts based on the cos-theta coil geometry with various stress management concepts and Bi2212 super-conducting strand and cable are being developed at Fermilab both within and beyond the U.S. national effort. On paper, the potential reach for the maximum magnetic field in existing or planned Nb3Sn outserts is close to 20 T, thanks to the progress realized in Bi2212 wires’ critical current density. To achieve the Bi2212 potential in accelerator magnets, however, a number of technological challenges still have to be faced. These for instance include the need to design billets that are adequate for Rutherford cabling; developing insulation processes and materials that prevent leaks, which reduce transport current and increase the risk of shorts; control and limit Bi2212 coils’ stresses and strains; reconsider the Split Melt Process (SMP) to lower costs and simplify the processing. This paper reviews Bi2212 conductor properties and coil technolo-gies, and proposes new ideas to face the challenges that Bi2212 still presents as an accelerator magnet conductor.

43 PARTICLE ACCELERATORS↗

Effects of mechanical stress and vitreous samples in retinal pigment epithelial cells

In rhegmatogenous retinal detachment (RRD), scattered RPE cells from the basement membrane into the vitreous cavity undergo an epithelial mesenchymal transition (EMT) and form the intraocular fibrous membrane in response to vitreous fluid. We investigated whether exposure to vitreous samples was associated with EMT-associated signals and mesenchymal characters. Human vitreous samples were collected from patients with RRD, epiretinal membrane (ERM), or macular hole (MH). We evaluated the effects of vitreous on ARPE-19 cells in suspension cultures using poly 2-hydroxyethyl methacrylate-coated dishes and three-dimensional (3D) Matrigel cultures. We found that exposure to vitreous samples did not induce morphological changes or accelerate wound closure in monolayers. Several samples showed increased phosphorylation of Smad2 and nuclear translocation of nuclear factor-κB. Mechanical stress triggered an elevation of phosphorylation levels in Smad2. In addition, exposure to vitreous fluid increased the phosphorylation of p38 mitogen-activated protein kinase in cell suspension cultures after mechanical stress. Moreover, ARPE-19 cells showed a stellate invasive phenotype in 3D Matrigel cultures with vitreous samples. In this study, we demonstrated that mechanical stress and vitreous were associated with EMT-associated signals and invasive phenotypes in 3D cultures but not in monolayers. These results have important implications for the role of vitreous humor in the induction of EMT and intraocular fibrosis.

60 APPLIED LIFE SCIENCES↗