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Space Biotechnology and Commercial Applications University of Florida

The Space Biotechnology and Commercial Applications grant was funded by NASA's Kennedy Space Center in FY 2002 to provide dedicated biotechnology and agricultural research focused on the regeneration of space flight environments with direct parallels in Earth-based applications for solving problems in the environment, advances in agricultural science, and other human support issues amenable to targeted biotechnology solutions. This grant had three project areas, each with multiple tasks. They are: 1) Space Agriculture and Biotechnology Research and Education, 2) Integrated Smart Nanosensors for Space Biotechnology Applications, and 3) Commercial Applications. The Space Agriculture and Biotechnology Research and Education (SABRE) Center emphasized the fundamental biology of organisms involved in space flight applications, including those involved in advanced life support environments because of their critical role in the long-term exploration of space. The SABRE Center supports research at the University of Florida and at the Space Life Sciences Laboratory (SLSL) at the Kennedy Space Center. The Integrated Smart Nanosensors for Space Biotechnology Applications component focused on developing and applying sensor technologies to space environments and agricultural systems. The research activities in nanosensors were coordinated with the SABRE portions of this grant and with the research sponsored by the NASA Environmental Systems Commercial Space Technology Center located in the Department of Environmental Engineering Sciences. Initial sensor efforts have focused on air and water quality monitoring essential to humans for living and working permanently in space, an important goal identified in NASA's strategic plan. The closed environment of a spacecraft or planetary base accentuates cause and effect relationships and environmental impacts. The limited available air and water resources emphasize the need for reuse, recycling, and system monitoring. It is essential to collect real-time information from these systems to ensure crew safety. This new class of nanosensors will be critical to monitoring the space flight environment in future NASA space systems. The Commercial Applications component of this program pursued industry partnerships to develop products for terrestrial use of NASA sponsored technologies, and in turn to stimulate growth in the biotechnology industry. For technologies demonstrating near term commercial potential, the objective is to include industry partners on or about the time of proof of concept that will not only co-invest in the technology but also take the resultant technology to the commercial market.

Phillips, Winfred↗

A large molecular cluster with high proton release capacity

We present a single molecular polyoxometalate cluster (K-41[(P2W12Nb6O62)(6){Mn-3(OH)(3)(H2O)(6)}(4){Mn3Na(H2O)(16)}]center dot 26H(2)O) with controllable release of a large number of protons (similar to 40 per molecule) in its aqueous solution upon addition of a base. The deprotonation/protonation process is reversible with the clusters remaining intact. This molecule can also absorb up to 11 protons per cluster when an acid, HCl, was added to its original aqueous solution. To the best of our knowledge, such large proton absorption/release capacity along with excellent stability is unprecedented.

Zhang, Dongdi↗

Iron isotope fractionation between solid and liquid metal in the Fe-P±Ni system: Experimental constraints and implications for meteorites

Iron meteorites record a range of Fe isotope compositions that hold valuable information regarding the evolution of their parent bodies. Interpreting this isotopic variability, however, requires experimental constraints on the equilibrium isotope fractionation between phases. It is thought that the cores of many iron meteorite parent bodies experienced fractional crystallization, during which crystallization of solid iron-nickel occurs from an increasingly non-metal-rich liquid alloy. Phosphorus is one component of this alloy, and this study provides the first constraints on Fe-isotope fractionation between solid and liquid alloys in the Fe-Ni-P system. Experiments comprising Fe and P show a clear enrichment in the light isotopes of Fe in the liquid phase, which increases with the amount of phosphorus. Nickel-bearing samples are offset from the trend defined by Ni-free experiments, which is accounted for by the change in the solid alloy phase from a body-centered cubic to face-centered cubic structure upon the addition of Ni. The increasing light isotope enrichment of the liquid with increasing P content suggests interstitial solution of P, which is known to lengthen Fe-Fe bonds in Fe-P liquids (Waseda and Shiraishi 1977). Results suggest a negligible effect of P on Fe isotope fractionation during planetesimal core crystallization. Iron isotopes may, however, prove useful for identifying the petrogenesis of schreibersite in pallasites and iron meteorites.

58 GEOSCIENCES↗

Foliar Spraying of Solanum tuberosum L. with CaCl2 and Ca(NO3)2: Interactions with Nutrients Accumulation in Tubers

Calcium is essential for plants, yet as its mobility is limited, the understanding of the rate of Ca2+ accumulation and deposition in tissues of tubers, as well as the interactions with other critical nutrients prompted this study. To assess the interactions and differential accumulation of micro and macronutrients in the tissues of tubers, Solanum tuberosum L. varieties Agria and Rossi were cultivated and, after the beginning of tuberization, four foliar sprayings (at 8–10 day intervals) with CaCl2 (3 and 6 kg ha−1) or Ca(NO3)2 (2 and 4 kg ha−1) solutions were performed. It was found that both fertilizers increased Ca accumulation in tubers (mostly in the parenchyma tissues located in the center of the equatorial region). The functioning of the photosynthetic apparatus was not affected until the 3rd application but was somewhat affected when approaching the end of the crop cycle (after the 4th application), although the lower dose of CaCl2 seemed to improve the photochemical use of energy, particularly when compared with the greater dose of Ca(NO3)2. Still, none of these impacts modified tuber height and diameter. Following the increased accumulation of Ca, in the tubers of both varieties, the mean contents of P, K, Na, Fe, and Zn revealed different accumulation patterns. Moreover, accumulation of K, Fe, Mn, and Zn prevailed in the epidermis, displaying a contrasting pattern relative to Ca. Therefore, Ca accumulation revealed a heterogeneous trend in the different regions analyzed, and Ca enrichment of tubers altered the accumulation of other nutrients.

Coelho, Ana Rita F. (ORCID:0000000339447240)↗

Lorentz Symmetry Group, Retardation, Intergalactic Mass Depletion and Mechanisms Leading to Galactic Rotation Curves

The general theory of relativity (GR) is symmetric under smooth coordinate transformations, also known as diffeomorphisms. The general coordinate transformation group has a linear subgroup denoted as the Lorentz group of symmetry, which is also maintained in the weak field approximation to GR. The dominant operator in the weak field equation of GR is thus the d’Alembert (wave) operator, which has a retarded potential solution. Galaxies are huge physical systems with dimensions of many tens of thousands of light years. Thus, any change at the galactic center will be noticed at the rim only tens of thousands of years later. Those retardation effects are neglected in the present day galactic modelling used to calculate rotational velocities of matter in the rims of the galaxy and surrounding gas. The significant differences between the predictions of Newtonian instantaneous action at a distance and observed velocities are usually explained by either assuming dark matter or by modifying the laws of gravity (MOND). In this paper, we will show that, by taking general relativity seriously without neglecting retardation effects, one can explain the radial velocities of galactic matter in the M33 galaxy without postulating dark matter. It should be stressed that the current approach does not require that velocities v are high; in fact, the vast majority of galactic bodies (stars, gas) are substantially subluminal—in other words, the ratio of $\frac{v}{c} \ll 1$. Typical velocities in galaxies are 100 km/s, which makes this ratio 0.001 or smaller. However, one should consider the fact that every gravitational system, even if it is made of subluminal bodies, has a retardation distance, beyond which the retardation effect cannot be neglected. Every natural system, such as stars and galaxies and even galactic clusters, exchanges mass with its environment, for example, the sun loses mass through solar wind and galaxies accrete gas from the intergalactic medium. This means that all natural gravitational systems have a finite retardation distance. The question is thus quantitative: how large is the retardation distance? For the M33 galaxy, the velocity curve indicates that the retardation effects cannot be neglected beyond a certain distance, which was calculated to be roughly 14,000 light years; similar analysis for other galaxies of different types has shown similar results. We demonstrate, using a detailed model, that this does not require a high velocity of gas or stars in or out of the galaxy and is perfectly consistent with the current observational knowledge of galactic and extra galactic material content and dynamics

79 ASTRONOMY AND ASTROPHYSICS↗

An efficient algorithm using matrix methods to solve wind tunnel force-balance equations

An iterative procedure applying matrix methods to accomplish an efficient algorithm for automatic computer reduction of wind-tunnel force-balance data has been developed. Balance equations are expressed in a matrix form that is convenient for storing balance sensitivities and interaction coefficient values for online or offline batch data reduction. The convergence of the iterative values to a unique solution of this system of equations is investigated, and it is shown that for balances which satisfy the criteria discussed, this type of solution does occur. Methods for making sensitivity adjustments and initial load effect considerations in wind-tunnel applications are also discussed, and the logic for determining the convergence accuracy limits for the iterative solution is given. This more efficient data reduction program is compared with the technique presently in use at the NASA Langley Research Center, and computational times on the order of one-third or less are demonstrated by use of this new program.

Smith, D. L.↗

Conductivity of a two-dimensional guiding center plasma.

The Kubo method is used to calculate the electrical conductivity of a two-dimensional, strongly magnetized plasma. The particles interact through (logarithmic) electrostatic potentials and move with their guiding center drift velocities (Taylor-McNamara model). The thermal equilibrium dc conductivity can be evaluated analytically, but the ac conductivity involves numerical solution of a differential equation. Both conductivities fall off as the inverse first power of the magnetic field strength.

Montgomery, D.↗

Resonance-line transfer with partial redistribution. VII Angle-dependent redistribution

A method is presented for treating radiative transfer in resonance lines, allowing for the full angle and frequency dependence of redistribution in the scattering process, as seen in the laboratory frame. The case of an equivalent-two-level-atom source function is considered; the problem to be treated is then linear in the radiation field. We apply this method to the Ca II lines in the solar atmosphere, using a redistribution function which takes into account a mixture of coherence in the atom's frame, with Doppler redistribution in the laboratory frame (for atoms which have not suffered an elastic collision), and of complete redistribution in the laboratory frame (for atoms that are collisionally perturbed during the emission process). Both the angle-averaged approximation and the full angle-dependent solution were obtained, and were compared to assess, differentially, the effects of angular redistribution upon the computed line profile and its center-to-limb behavior. For the Ca II line in a homogeneous solar chromosphere the angle-dependent effects are found to be negligible, indicating that one may use angle-averaged redistribution functions when studying partial redistribution effects in line profiles.

Milkey, R. W.↗

A detailed treatment of two-dimensional, starved lubrication in the vicinity of two counter-rotating cylinders

The problem of starved lubrication in the inlet region for the case of line contact between two nondeforming, equal radii cylinders rotating in opposite directions with equal surface speeds is considered. The lubricant is assumed to be a constant density, isoviscous, Newtonian fluid. The usual approach, which involves the use of Reynolds equation everywhere, is not followed. Instead, the situation is restricted to one in which the lubricant enters the contact region in thin films which adhere to the cylinders. Using Reynolds equation only at the line of centers and a suitable thin film approximation far from that point, the intervening region is treated as a general creeping flow region. Solutions, incorporating complete free surface boundary conditions, are obtained numerically. The hitherto unobtained results due to this approach include the existence of a unique free surface location for a fixed set of operating conditions.

Medrow, R. A.↗

Unstructured-grid methods development for unsteady aerodynamic and aeroelastic analyses

The current status of unstructured grid methods developed in the Unsteady Aerodynamics Branch at NASA Langley Research Center is described. These methods are being developed for unsteady aerodynamic and aeroelastic analyses. Flow solvers that have been developed for the solution of unsteady Euler equations are highlighted. The results demonstrate two and three dimensional applications for both steady and unsteady flows. Comparisons are also made with solutions obtained using a structured grid code and with experimental data to determine the accuracy of the unstructured grid methodology. These comparisons show good agreement which thus verifies the accuracy.

Batina, John T.↗

High performance VLSI telemetry data systems

NASA's deployment of major space complexes such as Space Station Freedom (SSF) and the Earth Observing System (EOS) will demand increased functionality and performance from ground based telemetry acquisition systems well above current system capabilities. Adaptation of space telemetry data transport and processing standards such as those specified by the Consultative Committee for Space Data Systems (CCSDS) standards and those required for commercial ground distribution of telemetry data, will drive these functional and performance requirements. In addition, budget limitations will force the requirement for higher modularity, flexibility, and interchangeability at lower cost in new ground telemetry data system elements. At NASA's Goddard Space Flight Center (GSFC), the design and development of generic ground telemetry data system elements, over the last five years, has resulted in significant solutions to these problems. This solution, referred to as the functional components approach includes both hardware and software components ready for end user application. The hardware functional components consist of modern data flow architectures utilizing Application Specific Integrated Circuits (ASIC's) developed specifically to support NASA's telemetry data systems needs and designed to meet a range of data rate requirements up to 300 Mbps. Real-time operating system software components support both embedded local software intelligence, and overall system control, status, processing, and interface requirements. These components, hardware and software, form the superstructure upon which project specific elements are added to complete a telemetry ground data system installation. This paper describes the functional components approach, some specific component examples, and a project example of the evolution from VLSI component, to basic board level functional component, to integrated telemetry data system.

Chesney, J.↗

Melt spinning study

Containerless processing of materials provides an excellent opportunity to study nucleation phenomena and produce unique materials, primarily through the formation of metastable phases and deep undercoolings. Deep undercoolings can be readily achieved in falling drops of molten material. Extended solute solubilities and greatly refined microstructures can also be obtained in containerless processing experiments. The Drop Tube Facility at Marshall Space Flight Center has played an important role in enhancing that area of research. Previous experiments performed in the Drop Tube with refractory metals has shown very interesting microstructural changes associated with deep undercoolings. It is apparent also that the microstructure of the deep undercooled species may be changing due to the release of the latent heat of fusion during recalescence. For scientific purposes, it is important to be able to differentiate between the microstructures of the two types of metallic species. A review of the literature shows that although significant advances have been made with respect to the engineering aspects of rapid solidification phenomena, there is still much to be learned in terms of understanding the basic phenomena. The two major ways in which rapid solidification processing provides improved structures and hence improved properties are: (1) production of refined structures such as fine dendrites and eutectics, and (2) production of new alloy compositions, microstructures, and phases through extended solid solubility, new phase reaction sequences, and the formation of metallic-glass microstructures. The objective of this work has been to determine the optimal methodology required to extract this excess energy without affecting the thermo-physical parameters of the under-cooled melt. In normal containerless processing experiments recalescence occurs as the melt returns toward the melting point in order to solidify. A new type of experiment is sought in which the resultant microstructure of the undercooled species is frozen in without going through the melting point regime and subsequent near equilibrium solidification of the remaining liquid. This experimental approach entails the design of an appropriate melt spinning system which is compatible with Drop Tube operations and processing constraints. That work is the goal of this study.

Workman, Gary L.↗

Astrometric studies in the region of Algol

The distance and masses of the components of the triple star Algol (Beta Persei) are derived from photographic and electronic data collected with the two 0.76 m objectives of the Thaw Refractor at the University of Pittsburgh's Allegheny Observatory and with the 0.61 m visual refractor at Swarthmore College's Sproul Observatory. These datasets constitute the major narrow-field astrometric collections of this star. The newly derived weighted mean trigonometric parallax of Algol is now +0.0343 +/- 0.00085 corresponding to a distance modulus of 2.32 +/- 0.054 mag. The total mass of the Algol system is estimated at 6.05 +/- 0.45 solar mass. The photocentric semimajor axis of the AB/C orbit is determined in the blue, yellow, and red bandpasses of the three independent astrometric systems. The total mass for the A and B component stars is found to be 4.63 +/- 0.35 solar mass and the mass of the C component is determined to be 1.42 +/- 0.13 solar mass. Residuals to this solution showed no meaningful correlation to the orbital motion of A/B. Thus it is probable that the photocenter is located near the center of mass of the A/B subsystem. There is no evidence of a previously suggested 32 yr orbital motion and no significant acceleration is evident in the 60 yr photographic series. It is therefore unlikely that the system has a fourth stellar mass component. It is noted that the visual band interferometric studies of the Algol system have the AB and C components interchanged.

Gatewood, George↗

Early events in the folding of an amphipathic peptide: A multinanosecond molecular dynamics study

Folding of the capped LQQLLQQLLQL peptide is investigated at the water-hexane interface by molecular dynamics simulations for 161.5 ns. Initially placed in the aqueous phase as a beta-strand, the peptide rapidly adsorbs to the interface, where it adopts an amphipathic conformation. The marginal presence of nonamphipathic structures throughout the complete trajectory indicates that the corresponding conformations are strongly disfavored at the interface. It is further suggestive that folding in an interfacial environment proceeds through a pathway of successive amphipathic intermediates. The energetic and entropic penalties involved in the conformational changes along this pathway markedly increase the folding time scales of LQQLLQQLLQL, explaining why the alpha-helix, the hypothesized lowest free energy structure for a sequence with a hydrophobic periodicity of 3.6, has not been reached yet. The formation of a type I beta-turn at the end of the simulation confirms the importance of such motifs as initiation sites allowing the peptide to coalesce towards a secondary structure. Proteins 1999;36:383-399. Copyright 1999 Wiley-Liss, Inc.

NASA Center ARC↗

Mitigating Motion Base Safety Issues: The NASA LaRC CMF Implementation

The NASA Langley Research Center (LaRC), Cockpit Motion Facility (CMF) motion base design has taken advantage of inherent hydraulic characteristics to implement safety features using hardware solutions only. Motion system safety has always been a concern and its implementation is addressed differently by each organization. Some approaches rely heavily on software safety features. Software which performs safety functions is subject to more scrutiny making its approval, modification, and development time consuming and expensive. The NASA LaRC's CMF motion system is used for research and, as such, requires that the software be updated or modified frequently. The CMF's customers need the ability to update the simulation software frequently without the associated cost incurred with safety critical software. This paper describes the CMF engineering team's approach to achieving motion base safety by designing and implementing all safety features in hardware, resulting in applications software (including motion cueing and actuator dynamic control) being completely independent of the safety devices. This allows the CMF safety systems to remain intact and unaffected by frequent research system modifications.

Bryant, Richard B., Jr.↗

Acoustical and Intelligibility Test of the Vocera(Copyright) B3000 Communication Badge

To communicate with each other or ground support, crew members on board the International Space Station (ISS) currently use the Audio Terminal Units (ATU), which are located in each ISS module. However, to use the ATU, crew members must stop their current activity, travel to a panel, and speak into a wall-mounted microphone, or use either a handheld microphone or a Crew Communication Headset that is connected to a panel. These actions unnecessarily may increase task times, lower productivity, create cable management issues, and thus increase crew frustration. Therefore, the Habitability and Human Factors and Human Interface Branches at the NASA Johnson Space Center (JSC) are currently investigating a commercial-off-the-shelf (COTS) wireless communication system, Vocera(C), as a near-term solution for ISS communication. The objectives of the acoustics and intelligibility testing of this system were to answer the following questions: 1. How intelligibly can a human hear the transmitted message from a Vocera(c) badge in three different noise environments (Baseline = 20 dB, US Lab Module = 58 dB, Russian Module = 70.6 dB)? 2. How accurate is the Vocera(C) badge at recognizing voice commands in three different noise environments? 3. What body location (chest, upper arm, or shoulder) is optimal for speech intelligibility and voice recognition accuracy of the Vocera(C) badge on a human in three different noise environments?

Archer, Ronald↗

High-Fidelity Analysis of Lift+Cruise VTOL Urban Air Mobility Concept Aircraft

This paper presents a high-fidelity multidisciplinary analysis for the NASA lift+cruise vertical takeoff and landing urban air mobility concept aircraft. The reported simulations couple comprehensive rotorcraft aeromechanics and high-fidelity computational fluid dynamics (CFD). Aerodynamic solutions are computed on dynamic, deforming, unstructured, overset grid systems by an unsteady CFD solver, FUN3D, developed at the NASA Langley Research Center. An integrated overset-grid assembler, Yoga, is used for communications between component grids. Two turbulence models are compared for loose-coupling simulations in hover and low-speed forward flight conditions. One model is the negative variant of the one-equation Spalart-Allmaras model (SA-neg), and the other model is the SA-neg-R model adding a simple rotation correction term to the SA-neg model. It demonstrated that the rotation correction in the SA-neg-R turbulence model significantly improves resolution of secondary vortices and wake interactions for rotorcraft simulations.

CFD↗

High-Fidelity Analysis of Lift+Cruise VTOL Urban Air Mobility Concept Aircraft

This paper presents a high-fidelity multidisciplinary analysis for the NASA Lift+Cruise vertical takeoff and landing urban air mobility concept aircraft. The reported simulations couple comprehensive rotorcraft aeromechanics and high-fidelity computational fluid dynamics (CFD). Aerodynamic solutions are computed on dynamic, deforming, unstructured, overset grid systems by an unsteady CFD solver, FUN3D, developed at the NASA Langley Research Center. An integrated overset-grid assembler, Yoga, is used for communications between component grids. Two turbulence models are compared for loose-coupling simulations in hover and low-speed forward flight conditions. One model is the negative variant of the one-equation Spalart-Allmaras model (SA-neg), and the other model is the SA-neg-R model adding a simple rotation correction term to the SA-neg model. It demonstrated that the rotation correction in the SA-neg-R turbulence model significantly improves resolution of secondary vortices and wake interactions for rotorcraft simulations.

CFD↗