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All sourcesNASA NTRSDOE OSTI
DOE OSTI2017

Age and Method of Release Affect Migratory Performance of Hatchery Steelhead

Abstract Hatchery programs that are designed to aid recovery of natural populations of anadromous salmonids, including steelhead Oncorhynchus mykiss, require locally derived, natural-origin broodstock. In such programs, achieving smoltification size thresholds may require extending hatchery rearing beyond age 1. We compared out-migration survival and travel rates of 142,990 PIT-tagged steelhead smolts released at age 1 (S1 rearing strategy) or age 2 (S2 rearing strategy) over five release years at Winthrop National Fish Hatchery (WNFH, Okanogan County, Washington). An S2 rearing cycle produced larger smolts with more uniform size distributions, resulting in higher survival during the first portion of their out-migration than for S1 smolts in three of the five release years. The S2 smolts migrated more rapidly to the ocean than S1 smolts in all years except 2011 and arrived in the Columbia River estuary 5.4 d earlier on average than the S1 smolts. The S1 steelhead that did not leave during the volitional release were subsequently forced from the hatchery to measure their survival. Nonvolitional S1 migrants were smaller and had survival rates that were 2.3–66.3 times lower than those of S1 steelhead that left WNFH on their own. The same was true for S2 steelhead, but the magnitude of the survival difference between volitional migrants and fish forced from the raceways was less variable and ranged from 2.5- to 4.6-fold. We conclude that S2 rearing can be a successful strategy for producing smolts from local natural-origin broodstock, with out-migration survival and travel times that are equivalent to or better than those of S1 smolts produced from nonlocal broodstock. Received July 25, 2016; accepted March 31, 2017 Published online May 31, 2017

Tatara, Christopher P.↗
DOE OSTI2019

Toward adaptive decision support for assessing infrastructure system resilience using hidden performance measures

The understanding of resilience is an emerging topic within the study of risks affecting distributed transportation infrastructure systems. Although recent studies have explored the quantification of system resilience, there has been limited research aimed at understanding the role of multiple performance measures, geographies, and modeling uncertainties within the assessment of resilience and associated decision-making. Under real-world conditions, there is an increased burden on analysts for translating observed system data (from human and electronic sensor observations) into the estimation of system performance that may not be directly observable. This paper addresses these issues using a scenario-based risk modeling approach to understand: 1) resilience of complex systems, often in cases of hidden (not readily observable) measures of performance, 2) resilience sensitivity to modeling uncertainties in event and system characteristics, and 3) resilience sensitivity to the measurement of performance across multiple dimensions. The methods in this paper integrate uncertainty-driven risk and probabilistic modeling within a multi-state Markov approach. This study contributes to the state-of-the-art by developing methodologies for assessing community perceptions of infrastructure system resilience using observable factors and inferring possibly hidden performance measures for facilitating adaptive decision-support. The methods are demonstrated with hypothetical spatiotemporal data for the Washington, District of Columbia and Baltimore, Maryland urban regions in the United States against evacuation-causing and shelter-in-place disruptive scenarios across multiple system performance dimensions. The analysis results may be useful for risk managers, supply chain analysts, emergency decision-makers, and the general public.

Infrastructure System Resilience, Risk Management,↗
DOE OSTI2015

A Synthesis of Findings from an Integrated Hatchery Program after Three Generations of Spawning in the Natural Environment

Abstract The Cle Elum Supplementation and Research Facility in the Yakima River basin, Washington, is an integrated spring Chinook Salmon Oncorhynchus tshawytscha hatchery program designed to test whether artificial propagation can increase natural production and harvest opportunities while keeping ecological and genetic impacts within acceptable limits. Only natural-origin (naturally spawned) fish are used for hatchery broodstock. Spawning, incubation, and early rearing occur at a central facility; presmolts are transferred for final rearing, acclimation, and volitional release at sites adjacent to natural spawning areas, where returning adults can spawn with natural-origin fish. The first wild broodstock were collected in 1997, and age-4 adults have returned to the Yakima River since 2001. An unsupplemented population in the adjacent Naches River watershed provides a reference for evaluating environmental influences. The program has been comprehensively monitored from its inception. A synthesis of findings, many already published, is as follows: supplementation increased the harvest, redd counts, and spatial distribution of spawners; natural-origin returns were maintained; straying to nontarget systems was negligible; natural-origin females had slightly higher breeding success (production of surviving fry) in an artificial spawning channel, while the behavior and breeding success of natural- and hatchery-origin males were similar; hatchery-origin fish showed differences in morphometric and life history traits; high rates of hatchery age-2 (minijack) production were reported, but the observed proportions of out-migrating juvenile and adult (ages 4 and 5) returning males were comparable for hatchery- and natural-origin fish; hatchery smolts did not affect the levels of pathogens in natural smolts; and the ecological interactions attributed to the program were within adopted guidelines. Continued study is required to assess the long-term impacts on natural production and productivity.

Fast, David E.↗
DOE OSTI2019

Progress Toward a Compact Fusion Reactor Using the Sheared-Flow-Stabilized Z-Pinch

The sheared-flow-stabilized (SFS) Z-pinch is a promising confinement concept for the development of a compact fusion reactor. The Z-pinch has been theoretically and experimentally shown to be stable to magnetohydrodynamic modes when sufficient radial shear of the axial flow is present. At the University of Washington, the Fusion Z-pinch Experiment (FuZE) research project examines scaling the SFS Z-pinch toward fusion conditions. The FuZE device produces long-duration, 50-cm-long pinches with measured ion and electron temperatures over 1 keV and number densities greater than 10 17 cm – 3 . Plasma properties are measured with a diagnostic suite that includes magnetic field probes, heterodyne quadrature interferometry, digital holographic interferometry, ion-Doppler spectroscopy, and fast framing photography. Neutrons are produced in the FuZE device when deuterium is injected along with the normal hydrogen or helium fueling species. Neutron generation is diagnosed using plastic scintillator detectors. The neutron production is sustained for 5 to 8 μs, thousands of times longer than the static Z-pinch instability growth time. We report measured neutron production is consistent with calculated theoretical values for thermonuclear yield at the observed plasma temperatures and scales with the square of the deuterium concentration. A preliminary reactor concept is designed to incorporate flowing liquid metal walls, which would serve as an electrode, a heat transfer fluid, a radiological shield, and a breeding blanket. Using a liquid metal wall could address several unresolved material and technology issues in existing fusion reactor designs.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2025

The ST40 Collaboration: A Groundbreaking, First-of-Its-Kind, Public-Private Partnership in Fusion

With the continued expansion of the private fusion sector, many governments are increasing support for public-private partnerships (PPPs) and positioning these partnerships as central to their plans to deliver commercial fusion. This paper discusses a groundbreaking first-of-its-kind PPP between the United Kingdom–based private company, Tokamak Energy Ltd. and the U.S. public sector researchers from the Princeton Plasma Physics Laboratory, Oak Ridge National Laboratory, and the University of Washington that focused on advancing spherical tokamak physics on the ST40, a high-field spherical tokamak owned and operated by Tokamak Energy. The collaboration was both scientifically productive and supportive of Tokamak Energy in achieving a key company milestone. Key factors that made this a productive and impactful collaboration are outlined from the perspectives of both the public and private participants. These principles can be used to inform future PPPs in fusion, and have already paved the way for new public-private initiatives.

Public-private partnership↗
DOE OSTI2016

Stock-Specific Size and Migration of Juvenile Coho Salmon in British Columbia and Southeast Alaska Waters

Abstract The variation at 17 microsatellites was analyzed for 5,270 juvenile Coho Salmon Oncorhynchus kisutch obtained from coastal British Columbia and Gulf of Alaska surveys during 1998–2012. A 270-population baseline was used to determine the individual identifications of the fish sampled, with individuals being identified to 22 stocks of origin. Columbia River and Washington juveniles were consistently larger than those from British Columbia and Alaska. During June, the larger individuals within a stock were observed in more northerly locations. There was a relationship between the timing of northward migration and juvenile body size, with larger individuals migrating earlier than smaller individuals from the same stocks. Stock composition was more diverse in the northern sampling regions than in those in southern British Columbia. There was only a modest change in stock composition between fall and winter samples in both the Strait of Georgia and west coast of Vancouver Island sampling regions, indicating that juvenile migration had largely been completed by the fall. There was a wide divergence among stocks in juvenile size and dispersion among sampling locations. Received December 9, 2015; accepted February 9, 2016

Beacham, Terry D.↗
DOE OSTI2013

DOUBLE STARS IN THE USNO CCD ASTROGRAPHIC CATALOG

The newly completed Fourth USNO CCD Astrographic Catalog (UCAC4) has proven to be a rich source of double star astrometry and photometry. Following initial comparisons of UCAC4 results against those obtained by speckle interferometry, the UCAC4 catalog was matched against known double stars in the Washington Double Star Catalog in order to provide additional differential astrometry and photometry for these pairs. Matches to 58,131 pairs yielded 61,895 astrometric and 68,935 photometric measurements. Finally, a search for possible new common proper motion (CPM) pairs was made using new UCAC4 proper motion data; this resulted in 4755 new potential CPM doubles (and an additional 27,718 astrometric and photometric measures from UCAC and other sources)

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2013

The solar neighborhood. XXX. Fomalhaut C

LP 876-10 is a nearby active M4 dwarf in Aquarius at a distance of 7.6 pc. The star is a new addition to the 10 pc census, with a parallax measured via the REsearch Consortium On Nearby Stars (RECONS) astrometric survey on the Small and Moderate Aperture Research Telescope System's 0.9 m telescope. We demonstrate that the astrometry, radial velocity, and photometric data for LP 876-10 are consistent with the star being a third bound stellar component to the Fomalhaut multiple system, despite the star lying nearly 6° away from Fomalhaut A in the sky. The three-dimensional separation of LP 876-10 from Fomalhaut is only 0.77 ± 0.01 pc, and 0.987 ± 0.006 pc from TW PsA (Fomalhaut B), well within the estimated tidal radius of the Fomalhaut system (1.9 pc). LP 876-10 shares the motion of Fomalhaut within ∼1 km s{sup –1}, and we estimate an interloper probability of ∼10{sup –5}. Neither our echelle spectroscopy nor astrometry are able to confirm the close companion to LP 876-10 reported in the Washington Double Star Catalog (WSI 138). We argue that the Castor Moving Group to which the Fomalhaut system purportedly belongs, is likely to be a dynamical stream, and hence membership to the group does not provide useful age constraints for group members. LP 876-10 (Fomalhaut C) has now risen from obscurity to become a rare example of a field M dwarf with well-constrained age (440 ± 40 Myr) and metallicity. Besides harboring a debris disk system and candidate planet, Fomalhaut now has two of the widest known stellar companions.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2014

Binary stars observed with adaptive optics at the starfire optical range

In reviewing observations taken of binary stars used as calibration objects for non-astronomical purposes with adaptive optics on the 3.5 m Starfire Optical Range telescope over the past 2 years, one-fifth of them were found to be off-orbit. In order to understand such a high number of discrepant position angles and separations, all previous observations in the Washington Double Star Catalog for these rogue binaries were obtained from the Naval Observatory. Adding our observations to these yields new orbits for all, resolving the discrepancies. We have detected both components of γ Gem for the first time, and we have shown that 7 Cam is an optical pair, not physically bound.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2022

From legacy contamination to watershed systems science: a review of scientific insights and technologies developed through DOE-supported research in water and energy security

Abstract Water resources, including groundwater and prominent rivers worldwide, are under duress because of excessive contaminant and nutrient loads. To help mitigate this problem, the United States Department of Energy (DOE) has supported research since the late 1980s to improve our fundamental knowledge of processes that could be used to help clean up challenging subsurface problems. Problems of interest have included subsurface radioactive waste, heavy metals, and metalloids (e.g. uranium, mercury, arsenic). Research efforts have provided insights into detailed groundwater biogeochemical process coupling and the resulting geochemical exports of metals and nutrients to surrounding environments. Recently, an increased focus has been placed on constraining the exchanges and fates of carbon and nitrogen within and across bedrock to canopy compartments of a watershed and in river–floodplain settings, because of their important role in driving biogeochemical interactions with contaminants and the potential of increased fluxes under changing precipitation regimes, including extreme events. While reviewing the extensive research that has been conducted at DOE’s representative sites and testbeds (such as the Oyster Site in Virginia, Savannah River Site in South Carolina, Oak Ridge Reservation in Tennessee, Hanford in Washington, Nevada National Security Site in Nevada, Riverton in Wyoming, and Rifle and East River in Colorado), this review paper explores the nature and distribution of contaminants in the surface and shallow subsurface (i.e. the critical zone) and their interactions with carbon and nitrogen dynamics. We also describe state-of-the-art, scale-aware characterization approaches and models developed to predict contaminant fate and transport. The models take advantage of DOE leadership-class high-performance computers and are beginning to incorporate artificial intelligence approaches to tackle the extreme diversity of hydro-biogeochemical processes and measurements. Recognizing that the insights and capability developments are potentially transferable to many other sites, we also explore the scientific implications of these advances and recommend future research directions.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Cropland abandonment between 1986 and 2018 across the United States: spatiotemporal patterns and current land uses

Knowing where and when croplands have been abandoned or otherwise removed from cultivation is fundamental to evaluating future uses of these areas, e.g. as sites for ecological restoration, recultivation, bioenergy production, or other uses. However, large uncertainties remain about the location and time of cropland abandonment and how this process and the availability of associated lands vary spatially and temporally across the United States. Here, we present a nationwide, 30 m resolution map of croplands abandoned throughout the period of 1986–2018 for the conterminous United States (CONUS). We mapped the location and time of abandonment from annual cropland layers we created in Google Earth Engine from 30 m resolution Landsat imagery using an automated classification method and training data from the U.S. Department of Agriculture Cropland Data Layer. Our abandonment map has overall accuracies of 0.91 and 0.65 for the location and time of abandonment, respectively. From 1986 to 2018, 12.3 (±2.87) million hectares (Mha) of croplands were abandoned across CONUS, with areas of greatest change over the Ogallala Aquifer, the southern Mississippi Alluvial Plain, the Atlantic Coast, North Dakota, northern Montana, and eastern Washington state. The average annual nationwide abandoned area across our study period was 0.51 Mha per year. Annual abandonment peaked between 1997 and 1999 at a rate of 0.63 Mha year –1 , followed by a continuous decrease to 0.41 Mha year –1 in 2009–2011. Among the abandoned croplands, 53% (6.5 Mha) changed to grassland and pasture, 18.6% (2.28 Mha) to shrubland and forest, 8.4% (1.03 Mha) to wetlands, and 4.6% (0.56 Mha) to non-vegetated lands. Of the areas that we mapped as abandoned, 19.6% (2.41 Mha) were enrolled in the Conservation Reserve Program as of 2020. Our new map highlights the long-term dynamic nature of agricultural land use and its relation to various competitive pressures and land use policies in the United States.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Mixing and dilution controls on marine CO 2 removal using alkalinity enhancement

Marine CO 2 removal (CDR) using enhanced-alkalinity seawater discharge was simulated in the estuarine waters of the Salish Sea, Washington, US. The high-alkalinity seawater would be generated using bipolar membrane electrodialysis technology to remove acid and the alkaline stream returned to the sea. Response of the receiving waters was evaluated using a shoreline resolving hydrodynamic model with biogeochemistry, and carbonate chemistry. Two sites, and two deployment scales, each with enhanced TA of 2997 mmol m -3 and a pH of 9 were simulated. The effects on air-sea CO 2 flux and pH in the near-field as well as over the larger estuary wide domain were assessed. The large-scale deployment (addition of 164 Mmoles TA yr -1 ) in a small embayment (Sequim Bay, 12.5 km 2 ) resulted in removal of 2066 T of CO 2 (45% of total simulated) at rate of 3756 mmol m -2 yr -1 , higher than the 63 mmol m -2 yr -1 required globally to remove 1.0 GT CO 2 yr -1 . It also reduced acidity in the bay, ΔpH ≈ +0.1 pH units, an amount comparable to the historic impacts of anthropogenic acidification in the Salish Sea. The mixing and dilution of added TA with distance from the source results in reduced CDR rates such that comparable amount 2176 T CO 2 yr -1 was removed over >1000 fold larger area of the rest of the model domain. There is the potential for more removal occurring beyond the region modeled. The CDR from reduction of outgassing between October and May accounts for as much as 90% of total CDR simulated. Of the total, only 375 T CO 2 yr -1 (8%) was from the open shelf portion of the model domain. With shallow depths limiting vertical mixing, nearshore estuarine waters may provide a more rapid removal of CO 2 using alkalinity enhancement relative to deeper oceanic sites.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Long-term patterns of post-fire harvest diverge among ownerships in the Pacific West, U.S.A.

Abstract Post-fire harvest (PFH) is a forest management practice designed to salvage value from burned timber, mitigate safety hazards from dead trees, reduce long-term fuels, and prepare sites for replanting. Despite public controversy and extensive ecological research, little is known about how much PFH occurs on private and public lands in the U.S. Pacific West, or how practices changed with shifting forest policy and increasing area burned over the last three decades. We mapped PFH across 2.2M burned hectares in California, Oregon, and Washington between 1986-2017 and used time series intervention analysis to compare trends in area, rate (% of burned area harvested), and mean patch size between private (0.5M ha) and federal (1.6M ha) forest land and across a gradient of burn severity. Harvest rates varied by ownership (4.9% federal, 18.6% private, 8.0% overall), and practices evolved and diverged over the study period. PFH area and rate declined across all ownerships in the mid-1990s during a period of reduced fire activity. As area burned increased between the early 2000s and late 2010s, PFH area rebounded and surpassed late-1980s levels, while rates remained relatively low. On federal lands, PFH practices shifted in the early-to-mid 1990s towards lower rates (10.3% to 3.8%) and smaller patches (6.0 to 3.3 ha), following policy changes and increased litigation. PFH rates on federal lands decreased at all levels of burn severity, with the largest decreases (6.2% to 1.2%) in forests with low tree mortality (i.e. fire refugia). Conversely, private PFH rates and mean patch sizes more than doubled in forests burned at very low-to-moderate severity. Our results highlight how PFH practices have shifted with policy, socio-economic pressure, and increasing area burned over 31 years in the Pacific West. A similar area of post-fire harvest is now dispersed over larger fires, with practices diverging substantially between ownerships.

Zuspan, Aaron (ORCID:0000000315833710)↗
DOE OSTI2024

Complexities of urban impacts on long-term seasonal trends in a mid-sized arid city

The variability of surface air temperature is of great importance for both society and the environment, is impacted by global warming and local-scale changes. In the arid eastern part of Washington state of USA, substantial urbanization has transformed the Tri-cities into the state’s third-largest urban cluster. This study utilizes a combination of in situ observations and reanalysis datasets to investigate the influence of land use changes on the region’s 2-meter temperature, revealing local effects that compensate for the background global warming. Within the urban fabric, distinct microclimates have emerged due to varying land use, establishing unique relationships between greenness and temperature alterations caused by land use transitions. Notably, our findings demonstrate that the observed compensating signal in the temperature of farmland locations is primarily attributed to local vegetation increases. Through these observations, this research highlights the urban impacts on local climate, offering valuable insights into the complexities surrounding land use and its consequences on the environment.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Estimating coccidioidomycosis endemicity while accounting for imperfect detection using spatio - temporal occupancy modeling

Coccidioidomycosis, or Valley fever, is an infectious disease caused by inhaling Coccidioides fungal spores. Incidence has risen in recent years, and it is believed the endemic region for Coccidioides is expanding in response to climate change. While Valley fever case data can help us understand trends in disease risk, using case data as a proxy for Coccidioides endemicity is not ideal because case data suffers from imperfect detection, including false positives (e.g., travel-related cases reported outside of endemic area) and false negatives (e.g., misdiagnosis or underreporting). Here we proposed a Bayesian, spatio-temporal occupancy model to relate monthly, county-level presence/absence data on Valley fever cases to latent endemicity of Coccidioides, accounting for imperfect detection. We used our model to estimate endemicity in the western United States. We estimated high probability of endemicity in southern California, Arizona, and New Mexico, but also in regions without mandated reporting, including western Texas, eastern Colorado, and southeastern Washington. We also quantified spatio-temporal variability in detectability of Valley fever, given an area is endemic to Coccidioides. We estimated an inverse relationship between lagged 3- and 9-month precipitation and case detection, and a positive association with agriculture. This work can help inform public health surveillance needs and identify areas that would benefit from mandatory case reporting.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2015

Migration depth and residence time of juvenile salmonids in the forebays of hydropower dams prior to passage through turbines or juvenile bypass systems: implications for turbine-passage survival

Little is known about the three-dimensional depth distributions in rivers of individually marked fish that are in close proximity to hydropower facilities. Knowledge of the depth distributions of fish approaching dams can be used to understand how vulnerable fish are to injuries such as barotrauma as they pass through dams. To predict the possibility of barotrauma injury caused by pressure changes during turbine passage, it is necessary to understand fish behaviour relative to acclimation depth in dam forebays as they approach turbines. A guiding study was conducted using high-resolution three-dimensional tracking results of salmonids implanted with Juvenile Salmon Acoustic Telemetry System transmitters to investigate the depth distributions of subyearling and yearling Chinook salmon ( Oncorhynchus tshawytscha ) and juvenile steelhead ( Oncorhynchus mykiss ) passing two dams on the Snake River in Washington State. Multiple approaches were evaluated to describe the depth at which fish were acclimated, and statistical analyses were performed on large data sets extracted from ~28 000 individually tagged fish during 2012 and 2013. Our study identified patterns of depth distributions of juvenile salmonids in forebays prior to passage through turbines or juvenile bypass systems. This research indicates that the median depth at which juvenile salmonids approached turbines ranged from 2.8 to 12.2 m, with the depths varying by species/life history, year, location (which dam) and diel period (between day and night). One of the most enlightening findings was the difference in dam passage associated with the diel period. The amount of time that turbine-passed fish spent in the immediate forebay prior to entering the powerhouse was much lower during the night than during the day. This research will allow scientists to understand turbine-passage survival better and enable them to assess more accurately the effects of dam passage on juvenile salmon survival.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2023

Spectral induced polarization monitoring of induced calcite precipitation in subsurface sediments

Co-precipitation of contaminants within the crystalline structure of calcite is a promising natural attenuation or remedial technology being considered at contaminated sites. We explore the sensitivity of the spectral induced polarization (SIP) method to induced calcite precipitation in natural sediments as a path forward to non-invasively monitor these sites. We performed time-lapse column experiments using phased (I-IV) injections over 40 days on natural sediments from the Hanford Site (Washington State, USA). In the phased injections, abiotic calcite precipitation was induced and confirmed to have occurred. Previous work in glass beads and homogeneous sand was limited to high frequency detection of calcite, however in this work we observed the development of two polarization mechanisms, one at high frequency (>100 Hz) and one at low frequency (< 100 Hz). Based on the characteristic frequencies from the SIP high and low frequency regimes, characteristic length scales (L) were computed where the adsorption mode of Na+ versus Ca2+ was compared by using diffusion coefficients corresponding to Na+ versus an arithmetically averaged value for Na+ and Ca2+. Using the diffusion coefficient of Na+, the high frequency L was found to correlate well with the size of the calcite crystals. The low frequency L correlated well with the individual natural sediment grain sizes within the columns. During late experimental times (day 36 and 40), the characteristic low frequency in two of the experimental columns shifted to lower frequencies (<0.001 Hz) which may signify SIP sensitivity of the formed calcite with the sediment grains. In field applications, the development of a low frequency polarization length scale to monitor calcite precipitation is promising for field monitoring applications, however further laboratory work needs to be performed to examine the SIP sensitivity of calcite formation in the presence of to natural sediments

calcium carbonate, calcite, precipitation, spectra↗
DOE OSTI2025

The reference genome for the northeastern Pacific bull kelp, Nereocystis luetkeana

Bull kelp, Nereocystis luetkeana, is a northeastern Pacific kelp with broad distribution from Alaska to central California. Its population declines have caused severe concerns in northern California, the Salish Sea in Washington, and recently in some populations in Oregon. Despite bull kelp's accumulated ecological and physiological studies, an assembled and annotated genomic reference was still unavailable. Here, we report the complete and annotated genome of Nereocystis luetkeana, produced by the California Conservation Genomics Project (CCGP), which aims to reveal genomic diversity patterns across California by sequencing the complete genomes of approximately 150 carefully selected species. The genome was assembled into 1562 scaffolds with 449.82 Mb, 80x of coverage and 22 952 gene models. BUSCO assembly showed a completeness score of 72% for the stramenopiles gene set. The mitochondria and chloroplast genome sequences have 37 Kb and 131 Mb, respectively. The orthology analysis between 10 Phaeophycean genomes showed 1065 expanded and 286 unique orthogroups for this species. Pairwise comparisons showed 542 orthogroups present only in N. luetkeana and M. pyrifera, another large-body kelp. The enrichment analysis of these orthogroups showed important functions related to central metabolism and signaling due to ATPases enrichment in these two species. This genome assembly will provide an essential resource for the ecology, evolution, conservation, and breeding of bull kelp.

California Conservation Genomics Project—CCGP↗