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NASA NTRS2019

Design, Prototyping, and Testing of a Novel Flowpath with an Array of Six 3D Matrix Vitvo Bioreactors for the NASA Bioculture System

The NASA Bioculture System is an advanced cell culture closed-loop system containing highly automated flowpaths designed to conduct long term biology experiments on ISS with earth remote controllable medium flow, temperature, gas composition, medium exchange, cell sampling and fixation. This technology was already demonstrated with successful cardiomyocyte and osteocyte cultures experiments onboard the ISS and is now supporting NASA PI science. The Bioculture System, however, can only support 10 cassettes with disposable flowpaths, each containing a single hollow fiber bioreactor with a culture capacity of about 2ml. This constraint not only severely limits the number of investigators that can conduct experiments in space, but also subjects the experiments to limitations in the number of replicates and conditions that can be studied. To address these limitations, we sought a novel design solution to maximize the number of separate bioreactor cultures and volume that can be conducted simultaneously. To this end we designed, prototyped, and are now testing a six-Vitvo 3D Matrix 2ml bioreactor insert that replaces the conventional Bioculture System hollow fiber bioreactor. This design will allow the Bioculture System to support up to 60 different bioreactors and samples at once. Specifically, the novel gas-tight containment housing insert contains six COTS Rigenerand VITVO bioreactors stacked on each side of a heat sink powered by the existing heating element and pair of temperature sensors. Medium will be distributed into each bioreactor's cell-free chamber via its built-in Luer connector, then across the 3D matrix to the cell chamber, dissipating laminar flow and limiting fluid shear stresses that might mechanostimulate cell cultures. Gas (5% CO2 in air) will be supplied directly to the bioreactor gas-tight housing for exchange via the bioreactor flat-surface gas-permeable membranes, eliminating the need for the existing Bioculture System cassette oxygenator. If successfully implemented on ISS, this new multi-bioreactor insert for the Bioculture System has the potential to make real-time cell science experimentation in space more efficient and accessible to more investigators.

Ma, Kristin R.↗

NASA NTRS2002

Mission design overview for the Mars Exploration Rover Mission

In the summer of 2003, NASA will launch the next wave of robotic explorers towards the planet Mars. Specifically designed to 'follow the water', two identical rovers wil be delivered to the surface of Mars to remotely conduct geologic and atmospheric investigations.

Mars Exploration Rover mission design↗

NASA NTRS1972

Development of a satellite microwave radiometer to sense the surface temperature of the world oceans

A proposed S-band radiometer for determining the ocean surface temperature with an absolute accuracy of + or - 1 Kelvin and a resolution of + or - .1 Kelvin was placed under the Advanced Applications Flight Experiment for further development into Nimbus readiness state. The results of assessing the following are described: effects due to the state of the sea surface, effects caused by the intervening atmosphere, and effects associated with imperfections in the instrument itself. An extensive sea truth program is also described for correlation of aircraft test flight measurements or of satellite remote measurement to in-situ data. An improved radiometer design is a modified Dicke-switch type with temperature stabilized, microwave integrated circuit, front-end and with a pulsed injection-noise nulling system. The radiometer has a multimode rectangular horn antenna with very low ohmic losses and a beam efficiency of 98% or better.

Hidy, G. M.↗

NASA NTRS1972

Short pulse radar measurements of layered ice and snow

Using a radar system with high range resolution, it was shown that measurements of snow and ice thickness are feasible with an accuracy of better than 3 inches. In addition, the water equivalent of the snowpack can be estimated, given a single surface measurement of the snow depth. Discontinuities in density were also detected and their location determined by inspection of the radar returns. The remote measurement of layer thickness is described. The system was designed for eventual incorporation into a light aircraft.

Vickers, R. S.↗

NASA NTRS1972

The census cities project: A status report for 1971

Work done by the Census Cities Project during 1971 is outlined. The project is designed to detect urban changes in land resource utilization from remote high altitude aircraft and earth orbiting satellite sensors, aid metropolitan regional planners, and provide urban land use maps for various areas. Land use maps are given of several areas observed during 1971.

Wray, J. R.↗

NASA NTRS1972

Central Atlantic regional ecological test site: A prototype regional environment information system

The author has identified the following significant results. Prior to the actual testing of data from ERTS-1, the principal scientific results to date are the establishment of a model or design for a regional land use information system based on remote sensor data and a firm establishment of the RB-57 type high altitude aircraft data base as an important tool in land use mapping. Preliminary user evaluations of the resulting land use data have been favorable, and one of the states, Maryland, has adopted the USGS-GAP land use system as a first step towards mapping their entire state. The State is funding an extension (to the west) of the part of Maryland not covered by CARETS (Central Atlantic Regional Ecological Test Site), using the GAP land use classification and mapping procedures.

Alexander, R. H.↗

NASA NTRS1972

Applications of remote sensing to stream discharge predictions

A feasibility study has been initiated on the use of remote earth observations for augmenting stream discharge prediction for the design and/or operation of major reservoir systems, pumping systems and irrigation systems. The near-term objectives are the interpolation of sparsely instrumented precipitation surveillance networks and the direct measurement of water loss by evaporation. The first steps of the study covered a survey of existing reservoir systems, stream discharge prediction methods, gage networks and the development of a self-adaptive variation of the Kentucky Watershed model, SNOPSET, that includes snowmelt. As a result of these studies, a special three channel scanner is being built for a small aircraft, which should provide snow, temperature and water vapor maps for the spatial and temporal interpolation of stream gages.

Krause, F. R.↗

NASA NTRS1974

Application of remote sensing to study nearshore circulation

The research to use remote sensing techniques for studying the continental shelf is reported. The studies reported include: (1) nearshore circulation in the vincinity of a natural tidal inlet; (2) identification of indicators of biological activity; (3) remote navigation system for tracking free drifting buoys; (4) experimental design of an estuaring tidal circulation; and (5) Skylab support work.

Zeigler, J.↗

NASA NTRS1975

Geological applications of LANDSAT-1 imagery to the Great Salt Lake area

The ERTS program has been designed as a research and development tool to demonstrate that remote sensing from orbital altitudes is a feasible and practical approach to efficient management of earth resources. From this synoptic view and repetitive coverage provided by ERTS imagery of the Great Salt Lake area, large geological and structural features, trends, and patterns have been identified and mapped. A comparative analysis of lineaments observed in September and December data was conducted, existing mineral locations were plotted, and areas considered prospective for mineralization based on apparent structure-mineralization relationships were defined. The additional information obtained using ERTS data provides an added source of information to aid in the development of more effective mineral exploration programs.

Anderson, A. T.↗

NASA NTRS1975

Soil moisture detection from Skylab

An investigation was designed for the Skylab satellite to determine the feasibility of remote sensing of the soil moisture content of the surface from various microwave sensors. Skylab data for the experiment were collected during passes 5, 10, 16, and 38 across the two test sites selected in eastern Kansas and western Texas. Pass 38 covered both test sites giving five data sets for the analysis. As Skylab data were being taken the moisture content of the soil was sampled by ground crews for each 2.5 centimeter depth from the surface to 15 centimeters at interval of about six kilometers along two different routes along the test sites. This resulted in a total of 2250 soil moisture samples corresponding to different locations and six different depths. Skylab data were collected by passive microwave radiometers at wavelengths of 2.1 and 21 centimeters by the S193 and S194 microwave sensors. An active microwave system also collected scatterometer data at a wavelength of 2.1 centimeters. The analysis of microwave data has revealed that the longer wavelength L-Band passive radiometer gives the best correlation with soil moisture content of the upper 2.5 centimeter depth of soil.

Eagleman, J. R.↗

NASA NTRS1978

Statistical sampling analysis for stratospheric measurements from satellite missions

Earth orbiting satellite experiments can be designed to measure stratospheric constituents such as ozone by utilizing remote sensing techniques. Statistical analysis techniques, mission simulation and model development have been utilized to develop a method for analyzing various mission/sensor combinations. Existing and planned NASA satellite missions such as Nimbus-4 and G, and Stratospheric Aerosol and Gas Experiment-Application Explorer Mission (SAGE-AEM) have been analyzed to determine the ability of the missions to adequately sample the global field.

Drewry, J. W.↗

NASA NTRS1979

Active/passive scanning

The paper deals with the design, construction, and applications of an active/passive multispectral scanner combining lasers with conventional passive remote sensors. An application investigation was first undertaken to identify remote sensing applications where active/passive scanners (APS) would provide improvement over current means. Calibration techniques and instrument sensitivity are evaluated to provide predictions of the APS's capability to meet user needs. A preliminary instrument design was developed from the initial conceptual scheme. A design review settled the issues of worthwhile applications, calibration approach, hardware design, and laser complement. Next, a detailed mechanical design was drafted and construction of the APS commenced. The completed APS was tested and calibrated in the laboratory, then installed in a C-47 aircraft and ground tested. Several flight tests completed the test program.

Woodfill, J. R.↗

NASA NTRS1980

Infrared-temperature variability in a large agricultural field

The combined effect of water carved gullies, varying soil color, moisture state of the soil and crop, nonuniform phenology, and bare spots was measured for commercially grown barley planted on varying terrain. For all but the most rugged terrain, over 80% of the area within 4, 16, 65, and 259 ha cells was at temperatures within 3 C of the mean cell temperature. The result of using relatively small, 4 ha instantaneous field of views for remote sensing applications is that either the worst or the best of conditions is often observed. There appears to be no great advantage in utilizing a small instantaneous field of view instead of a large one for remote sensing of crop canopy temperatures. The two alternatives for design purposes are then either a very high spatial resolution, of the order of a meter or so, where the field is very accurately temperature mapped, or a low resolution, where the actual size seems to make little difference.

Millard, J. P.↗

NASA NTRS1981

Progress of the LASSO experiment

The LASSO (Later Synchronisation from Stationary Orbit) experiment, designed to demonstrate the feasibility of achieving time synchronization between remote atomic clocks with an accuracy of one nanosecond or better by using laser techniques for the first time is described. The experiment uses groundbased laser stations and the SIRIO-2 geostationary satellite to be launched towards the end of 1981. The qualification of the LASSO on-board equipment is discussed with a brief description of the electrical and optical test equipment used. The progress of the operational organization is included.

Serene, B. E. H.↗

NASA NTRS1980

Kinesthetic coupling between operator and remote manipulator

A universal force-reflecting hand controller has been developed which allows the establishment of a kinesthetic coupling between the operator and a remote manipulator. The six-degree-of-freedom controller was designed to generate forces and torques on its three positional and three rotational axes in order to permit the operator to accurately feel the forces encountered by the manipulator and be as transparent to operate as possible. The universal controller has been used in an application involving a six-degree-of-freedom mechanical arm equipped with a six-dimensional force-torque sensor at its base. In this application, the hand controller acts as a position control input device to the arm, while forces and torques sensed at the base of the mechanical hand back drive the hand controller. The positional control relation and the back driving of the controller according to inputs experienced by the force-torque sensor are established through complex mathematical transformations performed by a minicomputer. The hand controller is intended as a development tool for investigating force-reflecting master-slave manipulator control technology.

Bejczy, A. K.↗

NASA NTRS1981

V/STOL propulsion control technology

Results of a NASA sponsored study of V/STOL Propulsion Control Analysis are presented. The study involved propulsion control requirements, design concepts and procedures, and control designs for supersonic V/STOL. A variable cycle engine with a remote augmented lift system was used as a basis for establishing typical operating requirements and control concepts, and a nonlinear engine model was developed for control development as a precursor to a real-time simulation capability. A simplified aircraft model was also used to investigate transition requirements, and a long-range technology plan was developed to define subsequent program requirements for achieving a real-time piloted simulation capability.

Brown, H.↗

NASA NTRS1981

Imaging spectroscopy; Proceedings of the Seminar, Los Angeles, CA, February 10, 11, 1981

The seminar concentrated on the science applications of spectral imaging, multispectral imaging sensors, and spectral filtering. Papers are presented on spectroscopic remote sensing for geological applications, spectral mapping of Jupiter, system design of an interferometer imaging sounder and a common module imaging spectral radiometer. Other topics discussed include the performance and application of an intensified linear self-scanned array instrument, tunable optical filtering using an interferometer for selective modulation, design studies for a spectrally agile staring sensor system, and acousto-optic tunable filters for high-resolution spectral analysis.

Norris, D. D.↗

NASA NTRS1988

A consideration of the use of optical fibers to remotely couple photometers to telescopes

The possible use of optical fibers to remotely couple photometers to telescopes is considered. Such an application offers the apparent prospect of enhancing photometric stability as a consequence of the benefits of remote operation and decreased sensitivity to image details. A properly designed fiber optic coupler will probably show no significant changes in optical transmisssion due to normal variations in the fiber configuration. It may be more difficult to eliminate configuration-dependent effects on the pupil of the transmitted beam, and thus achieve photometric stability to guiding and seeing errors. In addition, there is some evidence for significant changes in the optical throughputs of fibers over the temperature range normally encountered in astronomical observatories.

Heacox, William D.↗