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At least 901 records · Page 50

Comparison of DNA fragmentation and color thresholding for objective quantitation of apoptotic cells

Apoptosis is a process of cell death characterized by distinctive morphological changes and fragmentation of cellular DNA. Using video imaging and color thresholding techniques, we objectively quantitated the number of cultured CD4+ T-lymphoblastoid cells (HUT78 cells, RH9 subclone) displaying morphological signs of apoptosis before and after exposure to gamma-irradiation. The numbers of apoptotic cells measured by objective video imaging techniques were compared to numbers of apoptotic cells measured in the same samples by sensitive apoptotic assays that quantitate DNA fragmentation. DNA fragmentation assays gave consistently higher values compared with the video imaging assays that measured morphological changes associated with apoptosis. These results suggest that substantial DNA fragmentation can precede or occur in the absence of the morphological changes which are associated with apoptosis in gamma-irradiated RH9 cells.

Non-NASA Center↗

Near-Earth Object Astrometric Interferometry

Using astrometric interferometry on near-Earth objects (NEOs) poses many interesting and difficult challenges. Poor reflectance properties and potentially no significant active emissions lead to NEOs having intrinsically low visual magnitudes. Using worst case estimates for signal reflection properties leads to NEOs having visual magnitudes of 27 and higher. Today the most sensitive interferometers in operation have limiting magnitudes of 20 or less. The main reason for this limit is due to the atmosphere, where turbulence affects the light coming from the target, limiting the sensitivity of the interferometer. In this analysis, the interferometer designs assume no atmosphere, meaning they would be placed at a location somewhere in space. Interferometer configurations and operational uncertainties are looked at in order to parameterize the requirements necessary to achieve measurements of low visual magnitude NEOs. This analysis provides a preliminary estimate of what will be required in order to take high resolution measurements of these objects using interferometry techniques.

Werner, Martin R.↗

Resistance of Titanium Aluminide to Domestic Object Damage Assessed

A team consisting of GE Aircraft Engines, Precision Cast Parts, Oremet, and Chromalloy were awarded a NASA-sponsored Aerospace Industry Technology Program (AITP) to develop a design and manufacturing capability that will lead to the engine test demonstration and eventual implementation of a ?-Ti-47Al-2Nb-2Cr (at. %) titanium aluminide (TiAl) low-pressure turbine blade into commercial service. One of the main technical risks of implementing TiAl low-pressure turbine blades is the poor impact resistance of TiAl in comparison to the currently used nickel-based superalloy. The impact resistance of TiAl is being investigated at the NASA Lewis Research Center as part of the Aerospace Industry Technology Program and the Advanced High Temperature Engine Materials Program (HITEMP). The overall objective of this work is to determine the influence of impact damage on the high cycle fatigue life of TiAl-simulated low-pressure turbine blades. To this end, impact specimens were cast to size in a dog-bone configuration and given a typical processing sequence followed by an exposure to 650 degrees Celsius for 20 hours to simulate embrittlement at service conditions. Then, the specimens were impacted at 260 degrees Celsius under a 69-MPa load. Steel projectiles with diameters 1.6 and 3.2 mm were used to impact the specimens at 90 degrees Celsius to the leading edge. Two different impact energies (0.74 and 1.5 joules) were used to simulate fairly severe domestic object damage on a low-pressure turbine blade.

Lerch, Bradley A.↗

Evidence for Asteroidal Origin of the Tunguska Object

The progress in the understanding of the Tunguska object is reviewed in the light of evidence presented in numerous recent investigations, which appeared following the publication of my 1983 paper on the object's proposed asteroidal nature.

Tunguska tunguska fireball great balls of fire↗

Search for Near-Earth Object with Ground-Based Electro-Optical Deep Space Surveillance (GEODSS) Assets

This is a brief review of recent plans and current status of the adaptation of an Air Force wide field GEODSS telescope for the discovery and follow-up of Near-Earth Objects (NEOs). The proposed search program will focus on the discovery of natural objects and will proceed on a non-interference basis, i.e. avoiding any impact on GEODSS' dedicated purpose of tracking and monitoring Earth orbiting spacecraft. In September, 1992, JPL representatives had an initial meeting with GEODSS personnel and saw their facilities in Maui where use of GEODSS' telescopes were discussed in terms of using these existing sensors for the detection of NEOs. A result of these meetings was a test run at the GEODSS, Socorro, N. Mexico location to evaluate the tracking and astrometric accuracy of an upcoming close approach of NEA, (4179) Toutatis...

Helin, Eleanor F.↗

Low-thrust orbit transfer optimization with refined Q-law and multi-objective genetic algorithm

An optimization method for low-thrust orbit transfers around a central body is developed using the Q-law and a multi-objective genetic algorithm. in the hybrid method, the Q-law generates candidate orbit transfers, and the multi-objective genetic algorithm optimizes the Q-law control parameters in order to simultaneously minimize both the consumed propellant mass and flight time of the orbit tranfer. This paper addresses the problem of finding optimal orbit transfers for low-thrust spacecraft.

low-thrust orbit transfer↗

The Location of the CO2, Fundamental in Clathrate Hydrates and its Application to Infrared Spectra of Icy Solar System Objects

CO2 is present on the surface of many Solar System objects, but not always as a segregated, pure ice. In pure CO2-ice, the fundamental absorption is located near 4.268 micron (2343.3 wavenumbers). However, on several objects, the CO2 fundamental is shifted to higher frequency. This shift may be produced by CO2 gas trapped in another material, or adsorbed onto minerals. We have seen that a mixture of H2O, CH3OH4 and CO2 forms a type II clathrate when heated to 125 K and produces a CO2 fundamental near 4.26 micron. The exact location of the feature is strongly dependent on the initial ratio of the three components. We are currently exploring various starting ratios relevant to the Solar System to determine the minimum amount of CH3OH needed to convert all of the CO2 to the clathrate, i.e. eliminate the splitting of the CO2 fundamental. We are testing the stability of the clathrate to thermal processing and UV photolysis, and documenting the changes seen in the spectra in the wavelength range from 1-5 micron. We acknowledge financial support from the Origins of Solar Systems Program, the Planetary Geology and Geophysics and the NASA Postdoctoral Program.

Sandford, S. A.↗

Objective Lightning Probability Forecasting for Kennedy Space Center and Cape Canaveral Air Force Station

Five logistic regression equations were created that predict the probability of cloud-to-ground lightning occurrence for the day in the KSC/CCAFS area for each month in the warm season. These equations integrated the results from several studies over recent years to improve thunderstorm forecasting at KSC/CCAFS. All of the equations outperform persistence, which is known to outperform NPTI, the current objective tool used in 45 WS lightning forecasting operations. The equations also performed well in other tests. As a result, the new equations will be added to the current set of tools used by the 45 WS to determine the probability of lightning for their daily planning forecast. The results from these equations are meant to be used as first-guess guidance when developing the lightning probability forecast for the day. They provide an objective base from which forecasters can use other observations, model data, consultation with other forecasters, and their own experience to create the final lightning probability for the 1100 UTC briefing.

Lambert, Winifred↗

Scientific Exploration of Near-Earth Objects via the Crew Exploration Vehicle

The concept of a crewed mission to a Near-Earth Object (NEO) has been analyzed in depth in 1989 as part of the Space Exploration Initiative. Since that time two other studies have investigated the possibility of sending similar missions to NEOs. A more recent study has been sponsored by the Advanced Programs Office within NASA's Constellation Program. This study team has representatives from across NASA and is currently examining the feasibility of sending a Crew Exploration Vehicle (CEV) to a near-Earth object (NEO). The ideal mission profile would involve a crew of 2 or 3 astronauts on a 90 to 120 day flight, which would include a 7 to 14 day stay for proximity operations at the target NEO. One of the significant advantages of this type of mission is that it strengthens and validates the foundational infrastructure for the Vision for Space Exploration (VSE) and Exploration Systems Architecture Study (ESAS) in the run up to the lunar sorties at the end of the next decade (approx.2020). Sending a human expedition to a NEO, within the context of the VSE and ESAS, demonstrates the broad utility of the Constellation Program s Orion (CEV) crew capsule and Ares (CLV) launch systems. This mission would be the first human expedition to an interplanetary body outside of the cislunar system. Also, it will help NASA regain crucial operational experience conducting human exploration missions outside of low Earth orbit, which humanity has not attempted in nearly 40 years.

Abell, Paul A.↗

Solar Radiation Pressure Estimation and Analysis of a GEO Class of High Area-to-Mass Ratio Debris Objects

A population of deep space objects is thought to be high area-to-mass ratio (AMR) debris having origins from sources in the geosynchronous orbit (GEO) belt. The typical AMR values have been observed to range anywhere from 1's to 10's of m(sup 2)/kg, and hence, higher than average solar radiation pressure effects result in long-term migration of eccentricity (0.1-0.6) and inclination over time. However, the nature of the debris orientation-dependent dynamics also results time-varying solar radiation forces about the average which complicate the short-term orbit determination processing. The orbit determination results are presented for several of these debris objects, and highlight their unique and varied dynamic attributes. Estimation or the solar pressure dynamics over time scales suitable for resolving the shorter term dynamics improves the orbit estimation, and hence, the orbit predictions needed to conduct follow-up observations.

Kelecy, Tom↗

Methods and apparatus for constructing and implementing a universal extension module for processing objects in a database

Methods and apparatus for providing a multi-tier object-relational database architecture are disclosed. In one illustrative embodiment of the present invention, a multi-tier database architecture comprises an object-relational database engine as a top tier, one or more domain-specific extension modules as a bottom tier, and one or more universal extension modules as a middle tier. The individual extension modules of the bottom tier operationally connect with the one or more universal extension modules which, themselves, operationally connect with the database engine. The domain-specific extension modules preferably provide such functions as search, index, and retrieval services of images, video, audio, time series, web pages, text, XML, spatial data, etc. The domain-specific extension modules may include one or more IBM DB2 extenders, Oracle data cartridges and/or Informix datablades, although other domain-specific extension modules may be used.

Li, Chung-Sheng↗

Process for coating an object with silicon carbide

A process for coating a carbon or graphite object with silicon carbide by contacting it with silicon liquid and vapor over various lengths of contact time. In the process, a stream of silicon-containing precursor material in gaseous phase below the decomposition temperature of said gas and a co-reactant, carrier or diluent gas such as hydrogen is passed through a hole within a high emissivity, thin, insulating septum into a reaction chamber above the melting point of silicon. The thin septum has one face below the decomposition temperature of the gas and an opposite face exposed to the reaction chamber. The precursor gas is decomposed directly to silicon in the reaction chamber. A stream of any decomposition gas and any unreacted precursor gas from said reaction chamber is removed. The object within the reaction chamber is then contacted with silicon, and recovered after it has been coated with silicon carbide.

Levin, Harry↗

Statistical Estimation of Orbital Debris Populations with a Spectrum of Object Size

Orbital debris is a real concern for the safe operations of satellites. In general, the hazard of debris impact is a function of the size and spatial distributions of the debris populations. To describe and characterize the debris environment as reliably as possible, the current NASA Orbital Debris Engineering Model (ORDEM2000) is being upgraded to a new version based on new and better quality data. The data-driven ORDEM model covers a wide range of object sizes from 10 microns to greater than 1 meter. This paper reviews the statistical process for the estimation of the debris populations in the new ORDEM upgrade, and discusses the representation of large-size (greater than or equal to 1 m and greater than or equal to 10 cm) populations by SSN catalog objects and the validation of the statistical approach. Also, it presents results for the populations with sizes of greater than or equal to 3.3 cm, greater than or equal to 1 cm, greater than or equal to 100 micrometers, and greater than or equal to 10 micrometers. The orbital debris populations used in the new version of ORDEM are inferred from data based upon appropriate reference (or benchmark) populations instead of the binning of the multi-dimensional orbital-element space. This paper describes all of the major steps used in the population-inference procedure for each size-range. Detailed discussions on data analysis, parameter definition, the correlation between parameters and data, and uncertainty assessment are included.

Xu, Y. -l↗

Interpretation of the Near-IR Spectra of the Kuiper Belt Object

Visible and near-IR observations of the Kuiper Belt Object (136472) 2005 FY(9) have indicated the presence of unusually long (1 cm or more) optical path lengths in a layer of methane ice. Using microphysical and radiative transfer modeling, we show that even at the frigid temperatures in the outer reaches of the solar system, a slab of low porosity methane ice can indeed form by pressureless sintering of micron-sized grains, and it can qualitatively reproduce the salient features of the measured spectra. A good semiquantitative match with the near-IR spectra can be obtained with a realistic slab model, provided the spectra are scaled to a visible albedo of 0.6, at the low end of the values currently estimated from Spitzer thermal measurements. Consistent with previous modeling studies, matching spectra scaled to higher albedos requires the incorporation of strong backscattering effects. The albedo may become better constrained through an iterative application of the slab model to the analysis of the thermal measurements from Spitzer and the visible/near-IR reflectance spectra. The slab interpretation offers two falsifiable predictions (1) Absence of an opposition surge, which is commonly attributed to the fluffiness of the optical surface. This prediction is best testable with a spacecraft, as Earth-based observations at true opposition will not be possible until early next century. (2) Unlikelihood of the simultaneous occurrence of very long spectroscopic path lengths in both methane and nitrogen ice on the surface of any Kuiper Belt Object, as the more volatile nitrogen would hinder densification in methane ice.

Eluszkiewicz, Janusz↗

Tape Placement Head for Applying Thermoplastic Tape to an Object

A tape placement head for applying thermoplastic tape to an object includes a heated feeder which guides the tape/tow to a heated zone. The heated zone has a line compactor having a single row of at least one movable heated member. An area compactor is located in the heated zone downstream from the line compactor. The area compactor includes a plurality of rows of movable feet which are extendable toward the tape/tow different distances with respect to each other to conform to the shape of the object. A shim is located between the heated compactors and the tape/tow. A chilled compactor is in a chilled zone downstream from the heated zone. The chilled zone includes a line chilled compactor and an area chilled compactor. A chilled shim is mounted between the chilled compactor and the tape/tow.

Cope, Ralph D.↗

Toward an Objective Enhanced-V Detection Algorithm

The area of coldest cloud tops above thunderstorms sometimes has a distinct V or U shape. This pattern, often referred to as an "enhanced-V' signature, has been observed to occur during and preceding severe weather in previous studies. This study describes an algorithmic approach to objectively detect enhanced-V features with observations from the Geostationary Operational Environmental Satellite and Low Earth Orbit data. The methodology consists of cross correlation statistics of pixels and thresholds of enhanced-V quantitative parameters. The effectiveness of the enhanced-V detection method will be examined using Geostationary Operational Environmental Satellite, MODerate-resolution Imaging Spectroradiometer, and Advanced Very High Resolution Radiometer image data from case studies in the 2003-2006 seasons. The main goal of this study is to develop an objective enhanced-V detection algorithm for future implementation into operations with future sensors, such as GOES-R.

Brunner, Jason↗

Science Objectives of the JEM EUSO Mission on International Space Station

JEM-EUSO space observatory is planned with a very large exposure factor which will exceed the critical exposure factor required for observing the most of the sources within the propagational horizon of about one hundred Mpc. The main science objective of JEM-EUSO is the source-identifying astronomy in particle channel with extremey-high-energy particles. Quasi-linear tracking of the source objects through galactic magnetic field should become feasible at energy > 10(exp 20) eV for all-sky. The individual GZK profile in high statistics experiments should differ from source to source due to different distance unless Lorentz invariance is somehow limited. hi addition, JEM-EUSO has three exploratory test observations: (i), extremely high energy neutrinos beginning at E > 10(exp 19) eV: neutrinos as being expected to have a slowly increasing cross section in the Standard Model, and in particular, hundreds of times more in the extra-dimension models. (ii). fundamental physics at extreme Super LHC (Large Hadronic Collider) energies with the hierarchical unified energy much below the GUT scale, and (iii). global atmospheric observation, including large-scale and local plasma discharges, night-glow, meteorites, and others..

Takahashi, Yoshiyuki↗