Results for “time-lapse” — Page 5

NASA NTRS2020

Electric Six Degree of Freedom Installation Timelapse (Six Degree of Freedom Dynamic Test System)

The Six-degree-of-freedom Dynamic Test System (SDTS) is a short-range real-time motion based testing platform. Its primary use at NASA/JSC has been for development and qualification testing of docking systems, robotic assisted berthing mechanisms, and dynamic attachment systems. The SDTS is a closed-loop force feedback system that can simulate on-orbit contact dynamics between two vehicles utilizing the actual mechanical interfaces for testing. The facility is controlled by interconnected computers running real-time simulation software that takes measured contact forces, integrates the equations of motion in real time, and provide those states back into the motion table. This infrastructure can also be used for non-mating applications such as relative position sensor testing/evaluations. The SDTS facility provides a superstructure for mounting test articles, multiple test sensors, an intercom network for voice communications, and a synchronized timing system for data acquisition that includes HD video. This time-lapse video shows the buildup and testing of a modern electric actuation system created by Mikrolar, Inc. at the Johnson Space Center. This motion base will replace the current hydraulic actuator system, which is been in use since the 1970s, for all-future NASA testing. Once the system goes through customization to provide JSC’s unique closed-loop environment, the facility will help certify docking system and attach mechanisms on the ground before we send the first woman and the next man to the lunar surface

hexapod↗

NASA NTRSDate not supplied

U.S. Spacesuit Knowledge Capture – Chronicling Spacesuit Design for the Future

With less than 4 years until the United States is scheduled to land the first woman and next man on the Moon, NASA is leveraging 60 years of experience to build a spacesuit to assist in the success of this and future human space exploration missions. This experience comes from the achievements of retired and employed spacesuit experts, innovations that were conceived from existing ideas and inventions, and a plethora of archived knowledge. The U.S. Spacesuit Knowledge Capture (SKC) Program’s primary function is to capture, archive, and share current and legacy spacesuit-related knowledge with scientists, engineers, and technicians. To capture valuable spacesuit-related knowledge, the program uses various methods that have included hosting and recording classroom and online courses, workshops, and vignettes, and preserving thousands of legacy spacesuit-related files. In 2019, the SKC Program added to its role when it began coordinating the electronic recording of the new spacesuits’ buildup. This new, next-generation spacesuit is named the Exploration Extravehicular Mobility Unit (xEMU) and is a compilation of many components. As each component is tested and assembled into the suit, the SKC Program is chronicling this buildup using high-speed video production and photography that includes time-lapsed images. To complement the recording of the components, the SKC Program plans to record and photograph the design verification testing. In 2020, the SKC Program was given the initiative to research and identify the custodianship of historical spacesuit equipment that resides within the Crew and Thermal Systems Division. These archives will be added to the SKC Program’s expansive archived collection of spacesuit-related knowledge that represents over 5 decades of spacesuit legacy from the Apollo era to the pursuit of Mars and beyond. This paper describes the electronic documentation of the xEMU’s buildup and identifies the SKC Program’s 2020 accomplishments.

Cinda Chullen↗

NASA NTRSDate not supplied

U.S. Spacesuit Knowledge Capture – Chronicling Spacesuit Design for the Future

With less than 4 years until the United States is scheduled to land the first woman and next man on the Moon, NASA is leveraging 60 years of experience to build a spacesuit to assist in the success of this and future human space exploration missions. This experience comes from the achievements of retired and employed spacesuit experts, innovations that were conceived from existing ideas and inventions, and a plethora of archived knowledge. The U.S. Spacesuit Knowledge Capture (SKC) Program’s primary function is to capture, archive, and share current and legacy spacesuit-related knowledge with scientists, engineers, and technicians. To capture valuable spacesuit-related knowledge, the program uses various methods that have included hosting and recording classroom and online courses, workshops, and vignettes, and preserving thousands of legacy spacesuit-related files. In 2019, the SKC Program added to its role when it began coordinating the electronic recording of the new spacesuits’ buildup. This new, next-generation spacesuit is named the Exploration Extravehicular Mobility Unit (xEMU) and is a compilation of many components. As each component is tested and assembled into the suit, the SKC Program is chronicling this buildup using high-speed video production and photography that includes time-lapsed images. To complement the recording of the components, the SKC Program plans to record and photograph the design verification testing. In 2020, the SKC Program was given the initiative to research and identify the custodianship of historical spacesuit equipment that resides within the Crew and Thermal Systems Division. These archives will be added to the SKC Program’s expansive archived collection of spacesuit-related knowledge that represents over 5 decades of spacesuit legacy from the Apollo era to the pursuit of Mars and beyond. This paper describes the electronic documentation of the xEMU’s buildup and identifies the SKC Program’s 2020 accomplishments.

Cinda Chullen↗

NASA NTRSDate not supplied

Characterizing Antibiotic Resistance in Microgravity Environments

The gram-positive bacterium Staphylococcus aureus and the gram-negative Pseudomonas aeruginosa are two of NASA’s designated “medically important microorganisms” commonly co-isolated from spaceflight environments like the International Space Station (ISS). Prior studies demonstrate evidence of increased virulence and antibiotic resistance of each bacterium when grown as single species in space. “Characterizing Antibiotic Resistance in Microgravity Environments” (CARMEn) is an autonomous 30-day ISS payload experiment probing the phenotypic changes of S. aureus and P. aeruginosa biofilms grown together in microgravity. Phenotypic changes are probed through three parallel experiments measuring Extracellular Matrix (ECM) production, antibiotic tolerance, and RNA expression respectively. The ECM experiment uses time-lapse imaging to collect observational morphological data from biofilms stained with Congo Red and Coomassie Blue, the antibiotic tolerance experiment utilizes E-strip testing once samples return to Earth, and RNA expression is measured through RNAseq of samples treated with RNAProtect® during spaceflight.

Antibiotic resistance↗

NASA NTRSDate not supplied

Microbial Responses to Radiation at the Single-Cell Level

Exploring the biological effects of deep space radiation is crucial for developing protective protocols for humans traveling beyond Earth’s magnetosphere. Saccharomyces cerevisiae, or budding yeast, is an ideal model organism to study the effects of radiation on eukaryotes as it shares several pathways with humans, including DNA repair mechanisms. Here, we used time-lapse imaging of colony growth on agar plates to monitor the effects of radiation exposure on individual yeast cells and their progeny.

Paola J. Maldonado Martinez↗