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NASA NTRS1993

Ultrahigh resolution photographic films for X-ray/EUV/FUV astronomy

The quest for ultrahigh resolution full-disk images of the sun at soft X-ray/EUV/FUV wavelengths has increased the demand for photographic films with broad spectral sensitivity, high spatial resolution, and wide dynamic range. These requirements were made more stringent by the recent development of multilayer telescopes and coronagraphs capable of operating at normal incidence at soft X-ray/EUV wavelengths. Photographic films are the only detectors now available with the information storage capacity and dynamic range such as is required for recording images of the solar disk and corona simultaneously with sub arc second spatial resolution. During the Stanford/MSFC/LLNL Rocket X-Ray Spectroheliograph and Multi-Spectral Solar Telescope Array (MSSTA) programs, we utilized photographic films to obtain high resolution full-disk images of the sun at selected soft X-ray/EUV/FUV wavelengths. In order to calibrate our instrumentation for quantitative analysis of our solar data and to select the best emulsions and processing conditions for the MSSTA reflight, we recently tested several photographic films. These studies were carried out at the NIST SURF II synchrotron and the Stanford Synchrotron Radiation Laboratory. In this paper, we provide the results of those investigations.

Hoover, Richard B.↗
NASA NTRS2004

Fluorescent Approaches to High Throughput Crystallography

X-ray crystallography remains the primary method for determining the structure of macromolecules. The first requirement is to have crystals, and obtaining them is often the rate-limiting step. The numbers of crystallization trials that are set up for any one protein for structural genomics, and the rate at which they are being set up, now overwhelm the ability for strictly human analysis of the results. Automated analysis methods are now being implemented with varying degrees of success, but these typically cannot reliably extract intermediate results. By covalently modifying a subpopulation, less than or = 1%, of a macromolecule solution with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of a macromolecules purification. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination the crystals will show up as bright objects against a dark background. As crystalline packing is more dense than amorphous precipitate, the fluorescence intensity can be used as a guide in distinguishing different types of precipitated phases, even in the absence of obvious crystalline features, widening the available potential lead conditions in the absence of clear "bits." Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Also, brightly fluorescent crystals are readily found against less fluorescent precipitated phases, which under white light illumination may serve to obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries and by having the protein or protein structures all that show up. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons. This presentation will focus on the methodology for fluorescent labeling, the crystallization results, and the effects of the trace labeling on the crystal quality.

Minamitani, Elizabeth Forsythe↗
NASA NTRS2004

Fluorescent Approaches to High Throughput Crystallography

X-ray crystallography remains the primary method for determining the structure of macromolecules. The first requirement is to have crystals, and obtaining them is often the rate-limiting step. The numbers of crystallization trials that are set up for any one protein for structural genomics, and the rate at which they are being set up, now overwhelm the ability for strictly human analysis of the results. Automated analysis methods are now being implemented with varying degrees of success, but these typically can not reliably extract intermediate results. By covalently modifying a subpopulation, less than or = 1%, of a macromolecule solution with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of purification. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination the crystals show up as bright objects against a dark background. As crystalline packing is more dense than amorphous precipitate, the fluorescence intensity can be used as a guide in distinguishing different types of precipitated phases, even in the absence of obvious crystalline features, widening the available potential lead conditions in the absence of clear "hits." Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Also, brightly fluorescent crystals are readily found against less fluorescent precipitated phases, which under white light illumination may serve to obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries and by having the protein or protein structures all that show up. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons. This presentation will focus on the methodology for fluorescent labeling, the crystallization results, and the effects of the trace labeling on the crystal quality.

Pusey, Marc L.↗
NASA NTRS2005

Fluorescent Approaches to High Throughput Crystallography

X-ray crystallography remains the primary method for determining the structure of macromolecules. The first requirement is to have crystals, and obtaining them is often the rate-limiting step. The numbers of crystallization trials that are set up for any one protein for structural genomics, and the rate at which they are being set up, now overwhelm the ability for strictly human analysis of the results. Automated analysis methods are now being implemented with varying degrees of success, but these typically cannot reliably extract intermediate results. By covalently modifying a subpopulation, 51%, of a macromolecule solution with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of purification. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination the crystals show up as bright objects against a dark background. As crystalline packing is more dense than amorphous precipitate, the fluorescence intensity can be used as a guide in distinguishing different types of precipitated phases, even in the absence of obvious crystalline features, widening the available potential lead conditions in the absence of clear hits. Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Also, brightly fluorescent crystals are readily found against less fluorescent precipitated phases, which under white light illumination may serve to obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries and by having the protein or protein structures all that show up. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons. This presentation will focus on the methodology for fluorescent labeling, the crystallization results, and the effects of the trace labeling on the crystal quality.

Pusey, Marc L.↗
NASA NTRS2005

Fluorescent Approaches to High Throughput Crystallography

X-ray crystallography remains the primary method for determining the structure of macromolecules. The first requirement is to have crystals, and obtaining them is often the rate-limiting step. The numbers of crystallization trials that are set up for any one protein for structural genomics, and the rate at which they are being set up, now overwhelm the ability for strictly human analysis of the results. Automated analysis methods are now being implemented with varying degrees of success, but these typically cannot reliably extract intermediate results. By covalently modifying a subpopulation, less than or = 1 %, of a macromolecule solution with a fluorescent probe, the labeled material will add to a growing crystal as a microheterogeneous growth unit. Labeling procedures can be readily incorporated into the final stages of purification. The covalently attached probe will concentrate in the crystal relative to the solution, and under fluorescent illumination the crystals show up as bright objects against a dark background. As crystalline packing is more dense than amorphous precipitate, the fluorescence intensity can be used as a guide in distinguishing different types of precipitated phases, even in the absence of obvious crystalline features, widening the available potential lead conditions in the absence of clear "hits." Non-protein structures, such as salt crystals, will not incorporate the probe and will not show up under fluorescent illumination. Also, brightly fluorescent crystals are readily found against less fluorescent precipitated phases, which under white light illumination may serve to obscure the crystals. Automated image analysis to find crystals should be greatly facilitated, without having to first define crystallization drop boundaries and by having the protein or protein structures all that show up. The trace fluorescently labeled crystals will also emit with sufficient intensity to aid in the automation of crystal alignment using relatively low cost optics, further increasing throughput at synchrotrons. Preliminary experiments show that the presence of the fluorescent probe does not affect the nucleation process or the quality of the X-ray data obtained.

Pusey, Marc L.↗
NASA NTRS2017

A New Method of Absorption-Phase Nanotomography for 3D Observation of Mineral-Organic-Water Textiles and its Application to Pristine Carbonaceous Chondrites

Pristine carbonaceous chondrites contain fine-grained matrix, which is composed largely of amorphous silicates, sub-micron silicate and sulfide crystals, and organic materials. They are regarded as primitive dust in the early Solar System that have suffered minimal alteration in their parent bodies. The matrix generally has different lithologies; some of them are unaltered but some are more or less aqueously altered. Their textures have been examined in 2D usually by FE-SEM/EDS, TEM/EDS, nano-SIMS and micro-XRD. Observation of their complex fine textures, such as spatial relation between different lithologies in 3D, is important for understanding aggregation and alteration processes. Synchrotron radiation (SR)-based X-ray tomography reveals 3D structures nondestructively with high spatial resolution of approximately greater than 100 nm. We have developed a new technique using absorption contrasts called "dual-energy tomography" (DET) to obtain 3D distribution of minerals at SPring-8, SR facility in Japan, and applied successfully to Itokawa particles. Phase and absorption contrast images can be simultaneously obtained in 3D by using "scanning-imaging x-ray microscopy" (SIXM) at SPring-8, which can discriminate between void, water and organic materials. We applied this technique combined with FIB micro-sampling to carbonaceous chondrites to search for primitive liquid water. In this study, we combined the DET and SIXM to obtain three dimensional submicron-scale association between minerals, organic materials and water and applied this to pristine carbonaceous chondrites.

Tsuchiyama, A.↗
NASA NTRS2001

An XMM-Newton Study of 9SGR and the Lagoon Nebula

We report preliminary results of an XMM-Newton observation of the 04 V star 9 Sgr (= HD 164794). 9 Sgr is one of a few single OB stars that display a non-thermal radio emission attributed to synchrotron emission by relativistic electrons. Inverse Compton scattering of photospheric UV photons by these relativistic electrons is a priori expected to generate a non-thermal power-law tail in the X-ray spectrum. Our EPIC and RGS spectra of 9 Sgr suggest a more complex situation than expected from this 'simple' theoretical picture. Furthermore, soft-band EPIC images of the region around 9 Sgr reveal a number of point sources inside the Lagoon Nebula (M8). Most of these sources have optical counterparts inside the very young open cluster NGC 6530 and several X-ray sources are associated with low and intermediate mass pre-main sequence stars. Finally, we also detect (probably) diffuse X-ray emission from the Hourglass Region that might reveal a hot bubble blown by the stellar wind of Herschel 36, the ionizing star of the HG region.

Rauw, G.↗
NASA NTRS2015

Characterization of Meteorites by Focused Ion Beam Sectioning: Recent Applications to CAIs and Primitive Meteorite Matrices

Focused ion beam (FIB) sectioning has revolutionized preparation of meteorite samples for characterization by analytical transmission electron microscopy (TEM) and other techniques. Although FIB is not "non-destructive" in the purest sense, each extracted section amounts to no more than nanograms (approximately 500 cubic microns) removed intact from locations precisely controlled by SEM imaging and analysis. Physical alteration of surrounding material by ion damage, fracture or sputter contamination effects is localized to within a few micrometers around the lift-out point. This leaves adjacent material intact for coordinate geochemical analysis by SIMS, microdrill extraction/TIMS and other techniques. After lift out, FIB sections can be quantitatively analyzed by electron microprobe prior to final thinning, synchrotron x-ray techniques, and by the full range of state-of-the-art analytical field-emission scanning transmission electron microscope (FE-STEM) techniques once thinning is complete. Multiple meteorite studies supported by FIB/FE-STEM are currently underway at NASA-JSC, including coordinated analysis of refractory phase assemblages in CAIs and fine-grained matrices in carbonaceous chondrites. FIB sectioning of CAIs has uncovered epitaxial and other overgrowth relations between corundum-hibonite-spinel consistent with hibonite preceding corundum and/or spinel in non-equilibrium condensation sequences at combinations of higher gas pressures, dust-gas enrichments or significant nebular transport. For all of these cases, the ability of FIB to allow for coordination with spatially-associated isotopic data by SIMS provides immense value for constraining the formation scenarios of the particular CAI assemblage. For carbonaceous chondrites matrix material, FIB has allowed us to obtain intact continuous sections of the immediate outer surface of Murchison (CM2) after it has been experimentally ion processed to simulate solar wind space weathering. The surface amorphization and loss of OH produced by the irradiation provides important clues regarding space weathering on primitive asteroids such as the OSIRIS-Rex target 101955 Bennu.

Christoffersen, Roy↗
NASA NTRS1991

First results from the Faint Object Camera - Observations of PKS 0521 - 36

The Faint Object Camera on the Hubble Space Telescope was used to observe the radio galaxy PKS 0521 - 36 which hosts a prominent radio jet. Images of the jet show spatial structure comparable to VLA data and significantly better than optical ground-based observations. The jet structure is resolved at FOC resolution. In addition to the radio knot, well resolved by the FOC, an extension of the jet toward the nucleus is apparent. The rest of the jet does not show much clumpiness, implying that the synchrotron electrons must be accelerated all along the jet to account for the extent in the optical region.

Macchetto, F.↗
NASA NTRS1996

Interstellar Broadening of Images in the Gravitational Lens Pks 1830-211

The remarkably strong radio gravitational lens PKS 1830-211 consists of a one arcsecond diameter Einstein ring with two bright compact (milliarcsecond) components located on opposite sides of the ring. We have obtained 22 GHz VLBA data on this source to determine the intrinsic angular sizes of the compact components. Previous VLBI observations at lower frequencies indicate that the brightness temperatures of these components are significantly lower than 10(exp 10) K (Jauncey, et al. 1991), less than is typical for compact synchrotron radio sources and less than is implied by the short timescales of flux density variations. A possible explanation is that interstellar scattering is broadening the apparent angular size of the source and thereby reducing the observed brightness temperature. Our VLBA data support this hypothesis. At 22 GHz the measured brightness temperature is at least 10(exp 11) K, and the deconvolved 2 size of the core in the southwest compact component is proportional to upsilon(sup -2) between 1.7 and 22 GHz. VLBI observations at still higher frequencies should be unaffected by interstellar scattering.

Jones, D. L.↗
NASA NTRS2014

Binary Orbits as the Driver of Gamma-Ray Emission and Mass Ejection in Classical Novae

Classical novae are the most common astrophysical thermonuclear explosions, occurring on the surfaces of white dwarf stars accreting gas from companions in binary star systems. Novae typically expel about 10 (sup -4) solar masses of material at velocities exceeding 1,000 kilometers per second.However, the mechanism of mass ejection in novae is poorly understood, and could be dominated by the impulsive flash of thermonuclear energy, prolonged optically thick winds or binary interaction with the nova envelope. Classical novae are now routinely detected at giga-electronvolt gamma-ray wavelengths, suggesting that relativistic particles are accelerated by strong shocks in the ejecta. Here we report high-resolution radio imaging of the gamma-ray-emitting nova V959 Mon. We find that its ejecta were shaped by the motion of the binary system: some gas was expelled rapidly along the poles as a wind from the white dwarf, while denser material drifted out along the equatorial plane, propelled by orbital motion..At the interface between the equatorial and polar regions, we observe synchrotron emission indicative of shocks and relativistic particle acceleration, thereby pinpointing the location of gamma-ray production. Binary shaping of the nova ejecta and associated internal shocks are expected to be widespread among novae, explaining why many novae are gamma-ray emitters.

binary orbits as the driver of gamma-ray↗
NASA NTRS1992

Near-infrared images of the nuclear region of NGC 5128

High-resolution near-infrared images of NGC 5128 (Centaurus A) are presented, which probe the optically obscured nuclear region. The images show a central source at K (2.23 microns) and 3.26 microns. This central object is not prominent in the shorter wavelength images at J and H (1.23 and 1.65 micron, respectively), probably due to high extinction. The color images (H - K and J - H) provide evidence for a steep gradient in extinction across the array field. The images indicate three components: a red, variable, compact nucleus; the diffuse elliptical galaxy starlight; and a blue extension to the NE. The nucleus has decreased in flux density by a factor of 2.5 over less than about 5 yr at 3.26 microns. However, current spectral constraints on the nuclear source at near-infrared wavelengths cannot definitively determine the relative contributions from the known ratio synchrotron source (AGN) and from various possible dust-enshrouded thermal sources. The blue spot, approximately 7.6 +/- 0.1 arcsec NE of the nucleus appears to be a hole in the extinction, possibly enhanced by scattered light from the active galactic nucleus.

Turner, Paula C.↗
NASA NTRS1990

Infrared and optical imagery of the Crab Nebula

The paper presents 1.644 micron forbidden-line Fe II and 1.570 micron continuum images of the Crab Nebula which are compared with optical H-alpha, forbidden-line O III, and 5100 A continuum data. While the same prominent filaments are generally apparent in all lines, no substantial variations within the nebula in the strength and morphology of forbidden-line Fe II relative to the other lines are found. The infrared to optical continuum ratio of the synchrotron nebula shows 15 - 20 percent variations which are dominated by features which appear related to the position of the pulsar. These are interpreted as variations of 0.15 in the spectral index and it is suggested that they are related to the radiative decay of energetic particles and the paths over which these particles travel from the pulsar throughout the nebula.

Hester, J. Jeff↗
NASA NTRS1997

Deep WFPC2 and Ground-Based Imaging of a Complete Sample of 3C Quasars and Galaxies

We present the results of an HST and ground-based imaging study of a complete 3C sample of zeta approx. equal to 1 sources, comprising 5 quasars and 5 radio galaxies. We have observed all of the sample in essentially line-free bands at rest-frame 0.33 micrometers with WFPC2 and in rest-frame 1 micrometer images from the ground; we have also observed most of the sample in narrow-band filters centered on [O II]. We resolve continuum structure around all of our quasars in the high-resolution WFPC2 images, and in four of the five ground-based K' images. All of the quasars have some optical continuum structure that is aligned with the radio axis. In at least 3 of these cases, some of this optical structure is directly coincident with a portion of the radio structure, including optical counterparts to radio jets in 3C212 and 3C245 and an optical counterpart to a radio lobe in 3C2. These are most likely due to optical synchrotron radiation, and the radio and optical spectral indices in the northern lobe of 3C2 are consistent with this interpretation. The fact that we see a beamed optical synchotron component in the quasars but not in the radio galaxies complicates both the magnitude and the alignment comparisons. Nonetheless, the total optical and K' flux densities of the quasar hosts are consistent with those of the radio galaxies within the observed dispersion in our sample. The distributions of K' flux densities of both radio galaxies and quasar hosts exhibit similar mean and dispersion to that found for other radio galaxies at this redshift, and the average host galaxy luminosity is equivalent to, or a little fainter than, L*. The formal determination of the alignment in the optical and infrared in the two subsamples yields no significant difference between the radio galaxy and quasar subsamples, and the quasars 3C 196 and 3C 336 have aligned continuum and emission-line structure that is probably not due to beamed optical synchrotron emission. Very blue and/or edge-brightened structures are present in some objects within the probable quasar opening angle; these are possibly the result of illumination effects from the active nucleus, i.e., scattered quasar light or photoionization. In 3C 212, we see an optical object that lies 3 min. beyond the radio lobe, but which looks morphologically quite similar to the radio lobe itself. This object is bright in the infrared and has a steep spectral gradient along its length. A striking, semi-circular arc seen associated with 3C 280 may possibly be a tidal tail from a companion, enhanced in brightness by scattering or photoionization. In the near-infrared, most of the radio galaxies have elliptical morphologies with profiles that are well-fit by de Vaucouleurs r(exp 1/4)-laws and colors that are consistent with an old stellar population. All components around the quasars have optical-infrared colors that are redder than or similar to the colors of their respective nuclei; this is more consistent with a stellar origin for the emission than with a dominant scattering contribution. From the correspondence between the total magnitudes in the galaxies and quasars and the detection of aligned components in the quasars, we conclude that this study provides general support for the unification of FR II radio galaxies and quasars. Some of the objects in the sample (e.g, 3C 212) have properties that may be difficult to explain with our current understanding of the nature of FR II radio sources and the alignment effect.

Ridgway, Susan E.↗
NASA NTRS1988

The dual nature of the supernova remnant G351.2 + 0.1

The Galactic SNR G351.2 + 0.1 has been imaged at frequencies of 1.4, 5, and 15 GHz. The images reveal a source composed of two distinct components: a broken elongated shell measuring 4 x 6 arcmin and a central core about 15 arcsec in diameter. The two components are spectrally distinct. The shell has a nonthermal spectrum (alpha = -0.4) typical for Galactic SNRs, while the central core has an inverted spectrum (alpha = +0.27). The central core may be an analog of the Crab Nebula (a synchrotron nebula powered by a pulsar), but if so it is not typical of such objects.

Becker, R. H.↗
NASA NTRS1991

0.4-arc-second images of 3C 273

Images in V and I bands of the QSO 3C 273 (1226 + 023) were obtained using the High-Resolution Camera of the Canada-France-Hawaii telescope. Attention is given to the structure and colors of the jet and details of the host galaxy which have not been observed in previous studies. The systematic change of color along the jet suggests an aging process in a synchrotron spectrum, while the irregularity of the knots and wiggles suggests that the jet structure is primarily governed by passage through a clumpy medium near the host galaxy.

Hutchings, J. B.↗
NASA NTRS1983

Rapid X-ray variability from the Seyfert 1 Galaxy NGC 4051

Strong variable X-ray emission from the nearby low luminosity Seyfert 1 galaxy NGC 4051 was discovered during observations with the imaging proportional counter of the Einstein Observatory. During one 2304 second observation, the X-ray flux more than doubled in an approximately linear fashion, and a 70% increase for 150 seconds was seen during another 968 second observation. Evidence is presented which demonstrates that the X-ray spectrum of NGC 4051 is unusually soft compared to Seyfert 1 galaxies or QSOs. The emission mechanism is probably not synchrotron or synchrotron self-Compton, but the emission can be plausibly explained by various black hole accretion models.

Marshall, F. E.↗
NASA NTRS1983

Rapid X-ray variability from the Seyfert 1 galaxy NGC 4051

Strong variable X-ray emission from the nearby low luminosity Seyfert 1 galaxy NGC 4051 was discovered during observations with the imaging proportional counter of the Einstein Observatory. During one 2304 second observation, the X-ray flux more than doubled in an approximately linear fashion, and a 70 percent increase for 150 seconds was seen during another 968 second observation. Evidence is presented which demonstrates that the X-ray spectrum of NGC 4051 is unusually soft compared to Seyfert 1 galaxies or QSOs. The emission mechanism is probably not synchrotron or synchrotron self-Compton, but the emission can be plausibly explained by various black hole accretion models. Previously announced in STAR as N83-23265

Marshall, F. E.↗