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At least 91 records · Page 5

Reduced Artifact Approach for Determining Diffusion Coefficients in Time-Resolved Microscopy

Ultrafast microscopy methods traditionally assume a Gaussian profile to extract excited state diffusivities from transport measurements. Although this fitting method recovers accurate diffusion coefficients when the point spread function is well-represented by a Gaussian, even minor spatial aberrations introduced by the imaging system cause significant errors in the determined value. To provide a more accurate measure of excited state transport in nano- and microscale materials systems, in this work an alternative analysis protocol is proposed that numerically convolves the Green’s function solution to the diffusion equation with the experimentally measured point spread function. In contrast to the Gaussian fitting approach, the numerical convolution is shown to be robust against artifacts caused by nonideal point spread functions. Furthermore, the numerical convolution approach is highly effective at resolving anisotropic diffusion in modeled data.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Information-rich localization microscopy through machine learning

Recent years have witnessed the development of single-molecule localization microscopy as a generic tool for sampling diverse biologically relevant information at the super-resolution level. While current approaches often rely on the target-specific alteration of the point spread function to encode the multidimensional contents of single fluorophores, the details of the point spread function in an unmodified microscope already contain rich information. Here we introduce a data-driven approach in which artificial neural networks are trained to make a direct link between an experimental point spread function image and its underlying, multidimensional parameters, and compare results with alternative approaches based on maximum likelihood estimation. To demonstrate this concept in real systems, we decipher in fixed cells both the colors and the axial positions of single molecules in regular localization microscopy data.

42 ENGINEERING↗

Optimum constrained image restoration filters

The filter was developed in Hilbert space by minimizing the radius of gyration of the overall or composite system point-spread function subject to constraints on the radius of gyration of the restoration filter point-spread function, the total noise power in the restored image, and the shape of the composite system frequency spectrum. An iterative technique is introduced which alters the shape of the optimum composite system point-spread function, producing a suboptimal restoration filter which suppresses undesirable secondary oscillations. Finally this technique is applied to multispectral scanner data obtained from the Earth Resources Technology Satellite to provide resolution enhancement. An experimental approach to the problems involving estimation of the effective scanner aperture and matching the ERTS data to available restoration functions is presented.

Riemer, T. E.↗

Scintillator detector characterization for laser-driven proton beam imaging

The spatial resolution and imaging characteristics of plastic scintillators are characterized using laser-driven proton beams. Laser-driven proton beams typically have broad energy spectra and are accompanied by relativistic electrons and high-energy photons, both potentially contributing to background noise. Different types and thicknesses of Eljen Technology scintillators are compared to determine their intrinsic point spread function. Point-projection imaging of a mesh is used to compare the imaging resolution of the scintillator to the usual imaging detector, radiochromic film, and is found to be reasonably comparable and sufficient for many experimental applications.

47 OTHER INSTRUMENTATION↗

Report of subpanel on image sharpness

Factors effecting the sharpness of images acquired by multispectral scanners, synthetic aperture radar, thermal infrared sensors, and multispectral linear arrays are defined. Factors associated with the sensor include the atmospheric blur/scatter function, the point spread function of the sensor, the sensor aperture, the filter characteristics, and the sampling rate and quatization effects. Several research tasks are proposed which include investigating the use of optical prefilters which include investigating the use of optical prefilters and the relationships of the instantaneous field of view (aperture width) on the performance of geocorrelation systems.

Source record↗

Parameters Describing Earth Observing Remote Sensing Systems

The Earth science community needs to generate consistent and standard definitions for spatial, spectral, radiometric, and geometric properties describing passive electro-optical Earth observing sensors and their products. The parameters used to describe sensors and to describe their products are often confused. In some cases, parameters for a sensor and for its products are identical; in other cases, these parameters vary widely. Sensor parameters are bound by the fundamental performance of a system, while product parameters describe what is available to the end user. Products are often resampled, edge sharpened, pan-sharpened, or compressed, and can differ drastically from the intrinsic data acquired by the sensor. Because detailed sensor performance information may not be readily available to an international science community, standardization of product parameters is of primary performance. Spatial product parameters described include Modulation Transfer Function (MTF), point spread function, line spread function, edge response, stray light, edge sharpening, aliasing, ringing, and compression effects. Spectral product parameters discussed include full width half maximum, ripple, slope edge, and out-of-band rejection. Radiometric product properties discussed include relative and absolute radiometry, noise equivalent spectral radiance, noise equivalent temperature diffenence, and signal-to-noise ratio. Geometric product properties discussed include geopositional accuracy expressed as CE90, LE90, and root mean square error. Correlated properties discussed include such parameters as band-to-band registration, which is both a spectral and a spatial property. In addition, the proliferation of staring and pushbroom sensor architectures requires new parameters to describe artifacts that are different from traditional cross-track system artifacts. A better understanding of how various system parameters affect product performance is also needed to better ascertain the utility of existing datasets and products as well as to specify the performance of new sensors and products. Examples of simulations performed for the Landsat Data Continuity Mission illustrate how various parameters affect system and product performance. Specific examples include the effects of ground sample distance, MTF, and band-to-band registration on various products.

Zanoni, Vicki↗

Estimating Temperature Retrieval Accuracy Associated With Thermal Band Spatial Resolution Requirements for Center Pivot Irrigation Monitoring and Management

This study explores the use of synthetic thermal center pivot irrigation scenes to estimate temperature retrieval accuracy for thermal remote sensed data, such as data acquired from current and proposed Landsat-like thermal systems. Center pivot irrigation is a common practice in the western United States and in other parts of the world where water resources are scarce. Wide-area ET (evapotranspiration) estimates and reliable water management decisions depend on accurate temperature information retrieval from remotely sensed data. Spatial resolution, sensor noise, and the temperature step between a field and its surrounding area impose limits on the ability to retrieve temperature information. Spatial resolution is an interrelationship between GSD (ground sample distance) and a measure of image sharpness, such as edge response or edge slope. Edge response and edge slope are intuitive, and direct measures of spatial resolution are easier to visualize and estimate than the more common Modulation Transfer Function or Point Spread Function. For these reasons, recent data specifications, such as those for the LDCM (Landsat Data Continuity Mission), have used GSD and edge response to specify spatial resolution. For this study, we have defined a 400-800 m diameter center pivot irrigation area with a large 25 K temperature step associated with a 300 K well-watered field surrounded by an infinite 325 K dry area. In this context, we defined the benchmark problem as an easily modeled, highly common stressing case. By parametrically varying GSD (30-240 m) and edge slope, we determined the number of pixels and field area fraction that meet a given temperature accuracy estimate for 400-m, 600-m, and 800-m diameter field sizes. Results of this project will help assess the utility of proposed specifications for the LDCM and other future thermal remote sensing missions and for water resource management.

Ryan, Robert E.↗

LANDSAT-4 image data quality analysis

Methods were developed for estimating point spread functions from image data. Roads and bridges in dark backgrounds are being examined as well as other smoothing methods for reducing noise in the estimated point spread function. Tomographic techniques were used to estimate two dimensional point spread functions. Reformatting software changes were implemented to handle formats for LANDSAT-5 data.

Anuta, P. E.↗

Centroid tracking with area array detectors

A computer program (ALGEVAL) has been developed to simulate the position estimating behavior of a centroid estimator algorithm using data typical of optical point spread function data recorded by an area array detector. Typical results are shown of varying detector properties and optical point spread function types. The detector parameters currently available for study include read noise mean value, dark current mean value and spatial variation, charge transfer efficiency and point spread function location, saturation level, signal level and pixel size. The program is capable of calculating any order centroid using an array size from 2 x 2 to 15 x 15 pixels. The output of the program is either a performance map, histogram data or tabluar data. A number of further developments are recommended.

Glavich, T. A.↗

Characterizing degraded visual environments with scanning lenses to improve contrast recovery

Atmospheric aerosols, such as fog and smoke, degrade optical signals via the scattering and absorption of light, making it difficult to recover information from the surrounding environment. Here, we present a technique that measures the angular distribution of light in scattering environments to help recover these degraded optical signals. This is done by passing a collimated beam through the aerosol environment and collecting the scattered and unscattered light with an f-theta scan lens. The lens transforms the angular distribution of the scattered light into the linear domain, mapping the collected light onto a detector. The measured angular scattering distribution is then used to estimate the point spread function of the environment at an arbitrary stand-off distance. This point spread function can be used to deconvolve images blurred by the aerosol environment in which the angular scattering distribution was initially measured. With this approach, we demonstrate improvements in the effective resolution of deblurred images up to 26% over a 120-cm stand-off distance. Experimentation was performed in the Sandia National Laboratories tabletop fog chamber, a platform that generates aerosol environments analogous to real-world fogs. We intend to leverage this technique in future work to develop a low-SWaP system for the in situ characterization of aerosol environments.

Aerosols↗

Magnetoacoustic tomography with magnetic induction for high-resolution bioimepedance imaging through vector source reconstruction under the static field of MRI magnet

Purpose: Magnetoacoustic tomography with magnetic induction (MAT-MI) is an imaging modality to reconstruct the electrical conductivity of biological tissue based on the acoustic measurements of Lorentz force induced tissue vibration. This study presents the feasibility of the authors' new MAT-MI system and vector source imaging algorithm to perform a complete reconstruction of the conductivity distribution of real biological tissues with ultrasound spatial resolution. Methods: In the present study, using ultrasound beamformation, imaging point spread functions are designed to reconstruct the induced vector source in the object which is used to estimate the object conductivity distribution. Both numerical studies and phantom experiments are performed to demonstrate the merits of the proposed method. Also, through the numerical simulations, the full width half maximum of the imaging point spread function is calculated to estimate of the spatial resolution. The tissue phantom experiments are performed with a MAT-MI imaging system in the static field of a 9.4 T magnetic resonance imaging magnet. Results: The image reconstruction through vector beamformation in the numerical and experimental studies gives a reliable estimate of the conductivity distribution in the object with a ∼1.5 mm spatial resolution corresponding to the imaging system frequency of 500 kHz ultrasound. In addition, the experiment results suggest that MAT-MI under high static magnetic field environment is able to reconstruct images of tissue-mimicking gel phantoms and real tissue samples with reliable conductivity contrast. Conclusions: The results demonstrate that MAT-MI is able to image the electrical conductivity properties of biological tissues with better than 2 mm spatial resolution at 500 kHz, and the imaging with MAT-MI under a high static magnetic field environment is able to provide improved imaging contrast for biological tissue conductivity reconstruction.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Image Quality on the KAO

Three flights of the Kuiper Airborne Observatory (KAO) were taken to study the cause of the point spread function (PSF) degradation. The preliminary conclusions are: (1) the KAO point spread function decreases in size with elapsed time at altitude; (2) image motion is occasionally comparable to the size of the image, but is usually a sub-arcsecond effect; and (3) star images show small scale internal structural changes on a 200 microsecond timescale.

Dunham, E. W.↗

Measurements of microlens performance

We present results of laboratory evaluations of several microlens types that have been designed and fabricated at the Lockheed Research and Development Division. The microlenses include wideband and dispersive types, in isolation and in arrays, and fabricated with binary or grayscale methods. Different lens pixel geometries are considered, including square, hexagonal, and skewed microlenses. We describe our micro-optics laboratory testbed which has been designed for the evaluation of individual lenslets or 2D arrays at selected spectral wavelengths. Measurement capabilities include focal length, point-spread functions, wavefront quality, and modulation transfer functions. Our present effort focuses on the results of point spread function measurements and their comparison with design predictions.

Shough, D.↗

Circular multilayer zone plate for high-energy x-ray nano-imaging

A circular multilayer zone plate (MZP) was fabricated and its focusing performance was evaluated using 20-keV x-rays. MoSi{sub 2} and Si layers were alternately deposited by DC magnetron sputtering on a wire core; all the interfaces satisfied the Fresnel zone condition. The measured line spread function was converted to a point spread function by tomographic reconstruction. The results suggest that the MZP has the potential to realize the diffraction-limited resolving power, which is calculated to be 35 nm using the diffraction integral. Furthermore, scanning transmission microscopy using the MZP could resolve a 50-nm line-and-space pattern.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Interparameter trade-off quantification and reduction in isotropic-elastic full-waveform inversion: synthetic experiments and Hussar land data set application

The problem of inverting for multiple physical parameters in the subsurface using seismic full-waveform inversion (FWI) is complicated by interparameter trade-off arising from inherent ambiguities between different physical parameters. Parameter resolution is often characterized using scattering radiation patterns, but these neglect some important aspects of interparameter trade-off. More general analysis and mitigation of interparameter trade-off in isotropic-elastic FWI is possible through judiciously chosen multiparameter Hessian matrix-vector products. We show that products of multiparameter Hessian off-diagonal blocks with model perturbation vectors, referred to as interparameter contamination kernels , are central to the approach. We then apply the multiparameter Hessian to various vectors designed to provide information regarding the strengths and characteristics of interparameter contamination, both locally and within the whole volume. With numerical experiments, we observe that S-wave velocity perturbations introduce strong contaminations into density and phase-reversed contaminations into P-wave velocity, but themselves experience only limited contaminations from other parameters. Based on these findings, we introduce a novel strategy to mitigate the influence of interparameter trade-off with approximate contamination kernels. Furthermore, we recommend that the local spatial and interparameter trade-off of the inverted models be quantified using extended multiparameter point spread functions (EMPSFs) obtained with pre-conditioned conjugate-gradient algorithm. Compared to traditional point spread functions, the EMPSFs appear to provide more accurate measurements for resolution analysis, by de-blurring the estimations, scaling magnitudes and mitigating interparameter contamination. Approximate eigenvalue volumes constructed with stochastic probing approach are proposed to evaluate the resolution of the inverted models within the whole model. With a synthetic Marmousi model example and a land seismic field data set from Hussar, Alberta, Canada, we confirm that the new inversion strategy suppresses the interparameter contamination effectively and provides more reliable density estimations in isotropic-elastic FWI as compared to standard simultaneous inversion approach.

58 GEOSCIENCES↗

Spatial and spectral simulation of LANDSAT images of agricultural areas

A LANDSAT scene simulation capability was developed to study the effects of small fields and misregistration on LANDSAT-based crop proportion estimation procedures. The simulation employs a pattern of ground polygons each with a crop ID, planting date, and scale factor. Historical greenness/brightness crop development profiles generate the mean signal values for each polygon. Historical within-field covariances add texture to pixels in each polygon. The planting dates and scale factors create between-field/within-crop variation. Between field and crop variation is achieved by the above and crop profile differences. The LANDSAT point spread function is used to add correlation between nearby pixels. The next effect of the point spread function is to blur the image. Mixed pixels and misregistration are also simulated.

Pont, W. F., Jr.↗

Spatial and spectral simulation of Landsat images of agricultural areas

A Landsat scene simulation capability was developed to study the effects of small fields and misregistration on Landsat-based crop proportion estimation procedures. The simulation employs a pattern of ground polygons each with a crop ID, planting date, and scale factor. Historical greenness/brightness crop development profiles generate the mean signal values for each polygon. Historical within-field covariances add texture to pixels in each polygon. The planting dates and scale factors create between-field/within-crop variation. Between field and crop variation is achieved by the above and crop profile differences. The Landsat point spread function is used to add correlation between nearby pixels. The next effect of the point spread function is to blur the image. Mixed pixels and misregistration are also simulated. Previously announced in STAR as N82-32813

Pont, W. F., Jr.↗

A simple method for correcting spatially resolved solar intensity oscillation observations for variations in scattered light

A measurement of the intensity distribution in an image of the solar disk will be corrupted by a spatial redistribution of the light that is caused by the earth's atmosphere and the observing instrument. A simple correction method is introduced here that is applicable for solar p-mode intensity observations obtained over a period of time in which there is a significant change in the scattering component of the point spread function. The method circumvents the problems incurred with an accurate determination of the spatial point spread function and its subsequent deconvolution from the observations. The method only corrects the spherical harmonic coefficients that represent the spatial frequencies present in the image and does not correct the image itself.

Jefferies, S. M.↗