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NASA NTRS2011

C/NOFS Satellite Electric Field and Plasma Density Observations of Plasma Instabilities Below the Equatorial F-Peak -- Evidence for Approximately 500 km-Scale Spread-F "Precursor" Waves Driven by Zonal Shear Flow and km-Scale, Narrow-Banded Irregularities

As solar activity has increased, the ionosphere F-peak has been elevated on numerous occasions above the C/NOFS satellite perigee of 400km. In particular, during the month of April, 2011, the satellite consistently journeyed below the F-peak whenever the orbit was in the region of the South Atlantic anomaly after sunset. During these passes, data from the electric field and plasma density probes on the satellite have revealed two types of instabilities which had not previously been observed in the C/NOFS data set (to our knowledge): The first is evidence for 400-500km-scale bottomside "undulations" that appear in the density and electric field data. In one case, these large scale waves are associated with a strong shear in the zonal E x B flow, as evidenced by variations in the meridional (outward) electric fields observed above and below the F-peak. These undulations are devoid of smaller scale structures in the early evening, yet appear at later local times along the same orbit associated with fully-developed spread-F with smaller scale structures. This suggests that they may be precursor waves for spread-F, driven by a collisional shear instability, following ideas advanced previously by researchers using data from the Jicamarca radar. A second new result (for C/NOFS) is the appearance of km-scale irregularities that are a common feature in the electric field and plasma density data that also appear when the satellite is below the F -peak at night. The vector electric field instrument on C/NOFS clearly shows that the electric field component of these waves is strongest in the zonal direction. These waves are strongly correlated with simultaneous observations of plasma density oscillations and appear both with, and without, evidence of larger-scale spread-F depletions. These km-scale, quasi-coherent waves strongly resemble the bottomside, sinusoidal irregularities reported in the Atmosphere Explorer satellite data set by Valladares et al. [JGR, 88, 8025, 1983]. We interpret these new observations in terms of fundamental plasma instabilities associated with the unstable, nighttime equatorial ionosphere.

Pfaff, R.↗

NASA NTRS2012

Electric Field and Plasma Density Observations of Irregularities and Plasma Instabilities in the Low Latitude Ionosphere Gathered by the C/NOFS Satellite

The Vector Electric Field Investigation (VEFI) on the C/NOFS equatorial satellite provides a unique data set which includes detailed measurements of irregularities associated with the equatorial ionosphere and in particular with spread-F depletions. We present vector AC electric field observations gathered on C/NOFS that address a variety of key questions regarding how plasma irregularities, from meter to kilometer scales, are created and evolve. The talk focuses on occasions where the ionosphere F-peak has been elevated above the C/NOFS satellite perigee of 400 km as solar activity has increased. In particular, during the equinox periods of 2011, the satellite consistently journeyed below the F-peak whenever the orbit was in the region of the South Atlantic anomaly after sunset. During these passes, data from the electric field and plasma density probes on the satellite have revealed two types of instabilities which had not previously been observed in the C/NOFS data set: The first is evidence for 400-500km-scale bottomside "undulations" that appear in the density and electric field data. In one case, these large scale waves are associated with a strong shear in the zonal E x B flow, as evidenced by variations in the meridional (outward) electric fields observed above and below the F-peak. These undulations are devoid of smaller scale structures in the early evening, yet appear at later local times along the same orbit associated with fully-developed spread-F with smaller scale structures. This suggests that they may be precursor waves for spread-F, driven by a collisional shear instability, following ideas advanced previously by researchers using data from the Jicamarca radar. A second result is the appearance of km-scale irregularities that are a common feature in the electric field and plasma density data that also appear when the satellite is near or below the F-peak at night. The vector electric field instrument on C/NOFS clearly shows that the electric field component of these waves is strongest in the zonal direction. These waves are strongly correlated with simultaneous observations of plasma density oscillations and appear both with, and without, evidence of larger-scale spread-F depletions. These km-scale, quasi-coherent waves strongly resemble the bottomside, sinusoidal irregularities reported in the Atmosphere Explorer satellite data set by Valladares et al. and are believed to cause scintillations of VHF radiowaves. We interpret these new observations in terms of fundamental plasma instabilities associated with the unstable, nighttime equatorial ionosphere.

Pfaff, Robert F.↗

NASA NTRS1995

Cytoskeletal filament assembly and the control of cell spreading and function by extracellular matrix

This study was undertaken to analyze how cell binding to extracellular matrix produces changes in cell shape. We focused on the initial process of cell spreading that follows cell attachment to matrix and, thus, cell 'shape' changes are defined here in terms of alterations in projected cell areas, as determined by computerized image analysis. Cell spreading kinetics and changes in microtubule and actin microfilament mass were simultaneously quantitated in hepatocytes plated on different extracellular matrix substrata. The initial rate of cell spreading was highly dependent on the matrix coating density and decreased from 740 microns 2/h to 50 microns 2/h as the coating density was lowered from 1000 to 1 ng/cm2. At approximately 4 to 6 hours after plating, this initial rapid spreading rate slowed and became independent of the matrix density regardless of whether laminin, fibronectin, type I collagen or type IV collagen was used for cell attachment. Analysis of F-actin mass revealed that cell adhesion to extracellular matrix resulted in a 20-fold increase in polymerized actin within 30 minutes after plating, before any significant change in cell shape was observed. This was followed by a phase of actin microfilament disassembly which correlated with the most rapid phase of cell extension and ended at about 6 hours; F-actin mass remained relatively constant during the slow matrix-independent spreading phase. Microtubule mass increased more slowly in spreading cells, peaking at 4 hours, the time at which the transition between rapid and slow spreading rates was observed. However, inhibition of this early rise in microtubule mass using either nocodazole or cycloheximide did not prevent this transition. Use of cytochalasin D revealed that microfilament integrity was absolutely required for hepatocyte spreading whereas interference with microtubule assembly (using nocodazole or taxol) or protein synthesis (using cycloheximide) only partially suppressed cell extension. In contrast, cell spreading could be completely inhibited by combining suboptimal doses of cytochalasin D and nocodazole, suggesting that intact microtubules can stabilize cell form when the microfilament lattice is partially compromised. The physiological relevance of the cytoskeleton and cell shape in hepatocyte physiology was highlighted by the finding that a short exposure (6 hour) of cells to nocodazole resulted in production of smaller cells 42 hours later that exhibited enhanced production of a liver-specific product (albumin). These data demonstrate that spreading and flattening of the entire cell body is not driven directly by net polymerization of either microfilaments or microtubules.(ABSTRACT TRUNCATED AT 400 WORDS).

NASA Discipline Cell Biology↗

NASA NTRS1984

Dilution Jets in Accelerated Cross Flows

Results of flow visualization experiments and measurements of the temperature field produced by a single jet and a row of dilution jets issued into a reverse flow combustor are presented. The flow in such combustors is typified by transverse and longitudinal acceleration during the passage through its bending section. The flow visualization experiments were designed to examine the separate effects of longitudinal and transverse acceleration on the jet trajectory and spreading rate. A model describing a dense single jet in a lighter accelerating cross flow is developed. The model is based on integral conservation equations, including the pressure terms appropriate to accelerating flows. It uses a modified entrainment correlation obtained from previous experiments of a jet in a cross stream. The flow visualization results are compared with the model calculations in terms of trajectories and spreading rates. Each experiment is typified by a set of three parameters: momentum ratio, density ratio, and the densimetric Froude number. When injection velocities are large or densities are small, the Froude number becomes very large and hence, unimportant. Therefore, the Froude number is generally significant in describing liquid experiments but is unimportant for the gas experiments in the combustor. Agreement between test and calculated results is encouraging but tends to become poorer with increasing momentum ratio. The temperature measurements are presented primarily in the form of consecutive normalized temperature profiles. Some interpolated isothermal contours are also shown. The single jet trajectories are consistently found to be swept towards the inner wall of the bend, whether injection is from the outer or the inner wall. This behavior is explained by a drifting effect which consists of a transverse velocity component across the combustor due to the developing nature of the flow along it. Plots of lateral temperature distributions of the jet indicate that under longitudinal acceleration conditions the thermal spreading of the jet may be completely suppressed. Comparison between combustor experimental results and model calculations shows poor agreement due to the drifting effect which is not taken into consideration in the model calculations. The row of jets experiments are characterized by two additional parameters: spacing ratio and confinement parameter. The results are shown in the form of consecutive normalized temperature profiles. The confinement parameter appears to become increasingly important with decreasing spacing ratio, in particular when its effect is enhanced by the drifting phenomenon and associated pressure field. A tightly spaced row of jets injected from the inner wall, prior to the bend, is surprisingly kept attached to the inner wall in spite of the strong turning. A similar attachment for a jet injected from the outer wall is not observed.

Lipshitz, Abraham↗

NASA NTRS1974

Heat transfer and film cooling following injection through inclined circular tubes

Film cooling effectiveness and heat transfer are measured downstream of injection through discrete holes into a turbulent mainstream boundary layer. Air is injected through both a single hole and a row of holes spaced at three-diameter intervals and inclined at an angle of 35 deg to the main flow. There is little difference between the heat transfer coefficient with blowing and without blowing at low blowing rates (mass flux ratios). In fact, at low blowing rates, injection is found to decrease somewhat the heat transfer coefficient from that measured without blowing. As the mass flux ratio increases past unity, the heat transfer coefficient increases, especially with injection through a row of holes. The peak heat transfer is usually found at the edge of the spreading jets (i.e., between two holes). At a blowing rate near two, the lateral average of the heat transfer is as much as 27 percent higher than the heat transfer with no blowing. The increase in heat transfer is attributed to the interaction between the jets and the free stream, causing high levels of turbulence.

Eriksen, V. L.↗

NASA NTRS1959

Flight Studies of Problems Pertinent to High-Speed Operation of Jet Transports

A flight investigation was made to assess the potential operational problems of jet transports in the transonic cruise range. In this study a large multiengine jet airplane having geometric characteristics fairly representative of the jet transport was used; however, in order to ensure general applicability of the results, the aerodynamic characteristics of the test airplane were varied to simulate a variety of jet- transport airplanes. Some of the specific areas investigated include: (1) an overall evaluation of longitudinal stability and control characteristics at transonic speeds, with an assessment of pitch-up characteristics, (2) the effect of buffeting on airplane operational speeds and maneuvering, (3) the desirable lateral-directional damping characteristics, (4) the desirable lateral-control characteristics, (5) an assessment of over-speed and speed-spread requirements, including the upset maneuver, and (6) an assessment of techniques and airplane characteristics for rapid descent and slow-down. The results presented include pilots' evaluation of the various problem areas and specific recommendations for possible improvement of jet-transport operations in the cruising speed range.

Butchart, Stanley P.↗

NASA NTRS2019

A CME-Producing Solar Eruption from the Interior of an Emerging Bipolar Active Region

In a negative-polarity coronal hole, magnetic flux emergence, seen by the Solar Dynamics Observatory's {SDO) Helioseismic Magnetic lmager (HMI), begins at approximately 19:00 UT on March 3, 2016. The emerged magnetic field produced sunspots, which NOAA numbered 12514 two days later. The emerging magnetic field is largely bipolar with the opposite-polarity fluxes spreading apart overall, but there is simultaneously some convergence and cancellation of opposite-polarity flux at the polarity inversion line (PIL) inside the emerging bipole. In the first fifteen hours after emergence onset, three obvious eruptions occur, observed in the coronal EUV images from SDO's Atmospheric Imaging Assembly (AIA). The first two erupt from separate segments of the external PIL between the emerging positve-polarity flux and the extant surrounding negative-polarity flux, with the exploding magnetic field being prepared and triggered by flux cancellation at the external PIL. The emerging bipole shows obvious overall left-handed shear and/or twist in its magnetic field. The focus of th is poster is the third and largest eruption, which comes from inside the emerging bipole and blows it open to produce a CME observed by SOHO/LASCO. That eruption is preceded by flux cancellation at the emerging bipole's interior PIL, cancellation that plausibly builds a sheared and twisted flux rope above the interior PIL and finally triggers the blow-out eruption of the flux rope via photospheric-convection•driven slow tether-cutting reconnection of the legs of the sheared core field, low above the interior PIL, as proposed by van Ballegooijen & Martens (1989) and Moore & Roumeliotis (1992). The production of this eruption is a (perhaps rare) counterexample to solar eruptions that result from external collisional shearing between opposite polarities from two distinct emerging and/or emerged bipoles (Chintzoglou et al. 2019).

Adams, M. L.↗

NASA NTRS1989

Clotho Tessera, Venus: A fragment of Fortuna Tessera

Clotho Tessera, adjacent to southeast Lakshmi Planum, may provide additional evidence for lateral crustal motions, and a model for the origin of small tessera fragments. Clotho Tessera and Lakshmi Planum are so noticeably different, and in such close proximity, it is difficult to derive a reasonable model of their formation in situ. Squeezing of material out from beneath Lakshmi has been suggested as an origin for Moira Tessera, which is also adjacent to Lakshmi and 1400 km west of Clotho. However, a logical model of juxtaposition of the two different terrains, originally from points once distant, can be made for Clotho and Lakshmi (and perhaps other small tesserae as well). It is suggested that Clotho Tessera was once part of Fortuna Tessera, but was cut off by a transcurrent fault zone (the DLZ) striking perpendicular to the Sigrun rift and carried westward where it collided with Lakshmi Planum (forming Danu Montes). A gravity anomaly along the southern border of Lakshmi, in the area of Danu Montes, was interpreted as indicating subduction there, providing additional supporting evidence for the collision hypothesis. Diffusion of the DLZ with proximity to Sigrun Fossae may be due to either higher ductility near the postulated Sigrun rift, or to burial by flows away from the rift nearer to Valkyrie Fossae. Other possible examples of migrating tesserae occur elsewhere: small pieces of Ananke Tessera can be fit back together as though they had rifted apart, and the spreading apart of Ananke and Virilis Tesserae has been suggested because of their symmetric locations about the axis of an inferred spreading zone. Other tessera fragments appear to have been isolated by rifting, with little, if any, significant lateral motion (e.g., Meni and Tellus Tesserae, and Thethus and Fortuna Tesserae). The migrating terrain model for Clotho Tessera supports Sukhanov's interpretation of tesseral fragments as rafts of lighter crustal material.

Kozak, Richard C.↗

NASA NTRS2005

Discrete element simulations of gravitational volcanic deformation: Deformation structures and geometries - 1

We have carried out two-dimensional particle dynamics simulations of granular piles subject to frictional Coulomb failure criteria to gain a first-order understanding of different modes of gravitational deformation within volcanoes. Under uniform basal and internal strength conditions, granular piles grow self-similarly, developing distinctive stratigraphies, morphologies, and structures. Piles constructed upon cohesive substrates exhibit particle avalanching, forming outward dipping strata and angle of repose slopes. Systematic decreases in basal strength lead to progressively deeper and steeper internal detachment faults and slip along a basal decollement; landslide forms grade from shallow slumps to deep-seated landslide and, finally, to axial subsidence and outward flank displacements, or volcanic spreading. Surface slopes decrease and develop concave up morphologies with decreasing decollement strength; depositional layers tilt progressively inward. Spatial variations in basal strength cause lateral transitions in pile structure, stratigraphy, and morphology. This approximation of volcanoes as Coulomb granular piles reproduces the richness of deformational structures and surface morphologies in many volcanic settings. The gentle slopes of Hawaiian volcanoes and Olympus Mons on Mars suggest weak basal decollements that enable volcanic spreading. High-angle normal faults, favored above weak decollements, are interpreted in both settings and may explain catastrophic sector collapse in Hawaii and broad aureole deposits surrounding Olympus Mons. In contrast, steeper slopes and shallow detachment faults predominate in the Canary Islands, thought to lack a weak decollement, favoring smaller, more frequent slope failures than predicted for Hawaii. The numerical results provide a useful predictive tool for interpreting dynamic behavior and associated geologic hazards of active volcanoes.

Morgan, Julia K.↗

NASA NTRS2017

Summary of Propagation Cases of the Second AIAA Sonic Boom Prediction Workshop

A summary is provided for the propagation portion of the second AIAA Sonic Boom Workshop held January 8, 2017 in conjunction with the AIAA SciTech 2017 conference. Near-field pressure waveforms for two cases were supplied and ground signatures at multiple azimuthal angles as well as their corresponding loudness metrics were requested from 10 participants, representing 3 countries. Each case had some required runs, as well as some optional runs. The required cases included atmospheric profiles with measured data including winds, using Radiosonde balloon data at multiple geographically spread locations, while the optional cases included temperature and pressure profiles from the US Standard atmosphere. The humidity profiles provided for the optional cases were taken from ANSI guidance, as the authors were unaware of an accepted standard at the time the cases were released to the participants. Participants provided ground signatures along with the requested data, including some loudness metrics using their best practices, which included lossy as well as lossless schemes. All the participants' submissions, for each case, are compared and discussed. Noise or loudness measures are calculated and detailed comparisons and statistical analyses are performed and presented. It has been observed that the variation in the loudness measures and spread between participants' submissions increased as the computation proceeded from under-track locations towards the lateral cut-off. Lessons learned during this workshop are discussed and recommendations are made for potential improvements and possible subsequent workshops as we collectively attempt to refine our analysis methods.

Rallabhandi, Sriram↗

NASA NTRS2017

Summary of Propagation Cases of the Second AIAA Sonic Boom Prediction Workshop

A summary is provided for the propagation portion of the second AIAA Sonic Boom Workshop held January 8, 2017 in conjunction with the AIAA SciTech 2017 conference. Near-field pressure waveforms for two cases were supplied and ground signatures at multiple azimuthal angles as well as their corresponding loudness metrics were requested from 10 participants, representing 3 countries. Each case had some required, as well as some optional runs. The required cases included atmospheric profiles with measured data including winds, using Radiosonde balloon data at multiple geographically spread locations. The humidity profiles provided for the optional cases were taken from ANSI guidance, as the authors were unaware of an accepted standard at the time the cases were released to the participants. Participants provided ground signatures along with the requested data, including some loudness metrics using their best practices, which included lossy as well as lossless atmospheric propagation schemes. All the participants’ submissions, for each case, are compared and discussed. Noise or loudness measures are calculated and detailed comparisons and statistical analyses are performed and presented. It has been observed that the variation in the loudness measures and spread between participants’ submissions increased as the computation proceeded from under-track locations towards the lateral cut-off. Lessons learned during this workshop are discussed and recommendations are made for potential improvements and possible subsequent workshops as we collectively attempt to refine our analysis methods.

Rallabhandi, Sriram↗

NASA NTRSDate not supplied

Summary of Propagation Cases of the Third AIAA Sonic Boom Prediction Workshop

A summary is provided for the propagation portion of the Third AIAA Sonic Boom Prediction Workshop held January 5, 2020 in conjunction with the AIAA SciTech 2020 conference. Near-field pressure waveforms for two cases were supplied at multiple azimuthal angles. Ground signatures across the breadth of the sonic boom primary carpet and their corresponding loudness metrics were requested. Each case had some required, as well as some optional, runs. The required cases included atmospheric profiles with measured data including winds. Optional cases included a level-acceleration sonic boom focusing case as well as prediction under standard atmospheric conditions. Participants provided ground signatures along with several loudness metrics using their best sonic boom prediction practices. All the participants' submissions are compared and discussed for each case. Noise or loudness measures are calculated and detailed comparisons and statistical analyses are performed and presented. Compared to previous workshops, the selected cases in the current workshop have much lower boom loudness levels. As was observed in the past workshops, the variation in the loudness measures and spread between participants' submissions increased as the computation proceeded from under-track locations towards the lateral cut-off. Lessons learned during this workshop are discussed and recommendations are made for potential improvements and possible subsequent workshops as we collectively attempt to refine our analysis methods.

SBPW3↗

NASA NTRS1974

Hot spot and trench volcano separations

It is suggested that the distribution of separations between trench volcanos located along subduction zones reflects the depth of partial melting, and that the separation distribution for hot spot volcanoes near spreading centers provides a measure of the depth of mantle convection cells. It is further proposed that the lateral dimensions of mantle convection cells are also represented by the hot-spot separations (rather than by ridge-trench distances) and that a break in the distribution of hot spot separations at 3000 km is evidence for both whole mantle convection and a deep thermal plume origin of hot spots.

Lingenfelter, R. E.↗

NASA NTRS1980

Prediction of optical propagation losses through turbulent boundary/shear layers

A simplified mathematical model was developed which predicts the optical propagation losses which occur when an optical beam of given wave length passes through a turbulent boundary layer or shear layer. The optical losses are predicted in terms of line spread function (or Strehl ratio) and modulation transfer function by using experimentally determined values of layer thickness, streamwise, lateral and beamwise density fluctuation length scales, and distribution of the standard deviation of the density fluctuations through the turbulent layer. The prediction model was applied to the analysis of a number of selected cases of interest from the aerodynamic-optical interaction wind-tunnel investigation conducted in the NASA-Ames 1.83 x 1.83 meter (6 x 6 ft) wind tunnel. Direct optical measurements are compared with the results predicted by the aerodynamic analysis.

Verhoff, A.↗

NASA NTRS1989

Spread Of Charge From Ion Tracks In Integrated Circuits

Single-event upsets (SEU's) propagate to adjacent cells in integrated memory circuits. Findings of experiments in lateral transport of electrical-charge carriers from ion tracks in 256K dynamic randon-access memories (DRAM's). As dimensions of integrated circuits decrease, vulnerability to SEU's increases. Understanding gained enables design of less vulnerable circuits.

Zoutendyk, John A.↗

NASA NTRS2002

An amphioxus nodal gene (AmphiNodal) with early symmetrical expression in the organizer and mesoderm and later asymmetrical expression associated with left-right axis formation

The full-length sequence and zygotic expression of an amphioxus nodal gene are described. Expression is first detected in the early gastrula just within the dorsal lip of the blastopore in a region of hypoblast that is probably comparable with the vertebrate Spemann's organizer. In the late gastrula and early neurula, expression remains bilaterally symmetrical, limited to paraxial mesoderm and immediately overlying regions of the neural plate. Later in the neurula stage, all neural expression disappears, and mesodermal expression disappears from the right side. All along the left side of the neurula, mesodermal expression spreads into the left side of the gut endoderm. Soon thereafter, all expression is down-regulated except near the anterior and posterior ends of the animal, where transcripts are still found in the mesoderm and endoderm on the left side. At this time, expression also begins in the ectoderm on the left side of the head, in the region where the mouth later forms. These results suggest that amphioxus and vertebrate nodal genes play evolutionarily conserved roles in establishing Spemann's organizer, patterning the mesoderm rostrocaudally and setting up the asymmetrical left-right axis of the body.

Non-NASA Center↗

NASA NTRS2001

A spontaneously arising mutation in connexin32 with repeated passage of FRTL-5 cells coincides with increased growth rate and reduced thyroxine release

In this study we examine changes in the cellular properties of FRTL-5 cells as a function of passage number, with particular emphasis on gap junction expression, karyotype, morphology, growth rate and thyroxine (T(4)) release. Early passage FRTL-5 follicular cells transfer dye through gap junctions from injected cell(s) to third-order neighboring cells and beyond within their respective follicles and have immuno-detectable connexin32 (Cx32) type gap junctional plaques in their lateral contacting plasma membranes. By contrast, FRTL-5 cells established as monolayers, or as follicles from cultures passed more than 15 times, did not transfer microinjected Lucifer Yellow dye to contiguous neighboring cells and did not express any immuno-detectable rat thyroid specific connexins (Cx43, Cx32 or Cx26). Western blots confirmed that total, membrane and cytosolic Cx32 protein was present only in early pass follicular cultures. To better understand the passage-dependent loss of Cx32 expression, RT-PCR primers were made to the most unique sequences of the rat Cx32 molecule, the cytoplasmic and carboxyl-terminal regions. These primers were used to screen FRTL-5 RNA from cultures of various passage numbers. The results revealed that later passage cultures had a single base deletion in the middle of the Cx32 cytoplasmic loop region at nucleotide position 378. This base deletion was in the middle position of the codon for amino acid 116, which is normally a CAC (histidine) but read with the frame shift was a CCC (proline). The four amino acids that followed this deletion were also altered with the fourth one becoming UAA, the ochre translation stop codon. This premature stopping of translation resulted in a truncation of 60% of the protein, which included the remaining cytoplasmic loop, third and fourth transmembrane regions and the carboxyl-terminus. The later passage cultures did not produce a carboxyl-terminal RT-PCR product, indicating that the mRNA was also truncated. These regions of the Cx32 molecule contain the sequences and epitopes to which probes and antibodies are directed, and as such alterations of these regions with repeated passage explains reports by others that FRTL-5 cells do not express Cx32, and implies that cultures used for these assessments were passed more than 15 times. To determine if genetic or epigenetic abnormalities existed in FRTL-5 cells we performed chromosome spreads from various passage cultures. FRTL-5 cells have been reported to be diploid and more recently non-diploid; however, we found them to be fully tetraploid. This tetraploidy appears to be unstable in that later passes are tetraploid plus two or three extra chromosomes. There were no obvious translocations, breaks or large-scale interstitial deletions of any chromosomes in the FRTL-5 cultures tested. As FRTL-5 cells were repeatedly passed their morphology changed. Monolayer areas spread from beneath the follicles, and the follicles became flattened in appearance. These physical changes were coincident with dramatically increased growth rates. Early cultures (passed 3-12 times) divided on average every 49+/-1 h, whereas later passes (passes 20-25) divided every 28+/-3 h. To correlate these changes with a measure of thyroid function we assayed T(4) output. Early passage follicular cultures incubated for 6 h with sodium iodide, released on average 5.27+/- 0.33 ng/ml of T(4)/100 follicles. Later passes, or early passes treated with heptanol to down-regulate Cx32, released an average of 3.84+/-0.50 ng/ml of T(4)/100 follicles. There was a 27% difference in T(4) release between early follicular cultures, that were coupled by Cx32, and late or down-regulated early follicular cultures, that were uncoupled (P<0.0001). Collectively, the physical changes documented in this study were coincident with the loss of functional Cx32. This implies a relationship between the loss of intercellular communication and changes in morphogenic appearance, growth rate and reduced thyroid function and supports the previously postulated, tumor-suppressor role for Cx32. FRTL-5 cultures from low passage numbers are an excellent model of primary thyroid cells. However, many reports in the literature ascribe features to FRTL-5 cells that are mutually inconsistent. These differences may be resolved in the future by addressing the passage number and the conditional differences of the cultures being studied.

NASA Discipline Radiation Health↗

NASA NTRS2001

The Vertical Error Characteristics of GOES-derived Winds: Description and Impact on Numerical Weather Prediction

Errors in the height assignment of some satellite-derived winds exist because the satellites sense radiation emitted from a finite layer of the atmosphere rather than a specific level. Potential problems in data assimilation may arise because the motion of a measured layer is often represented by a single-level value. In this research, cloud and water vapor motion winds that are derived from the Geostationary Operational Environmental Satellites (GOES winds) are compared to collocated rawinsonde observations (RAOBs). An important aspect of this work is that in addition to comparisons at each assigned height, the GOES winds are compared to the entire profile of the collocated RAOB data to determine the vertical error characteristics of the GOES winds. The impact of these results on numerical weather prediction is then investigated. The comparisons at individual vector height assignments indicate that the error of the GOES winds range from approx. 3 to 10 m/s and generally increase with height. However, if taken as a percentage of the total wind speed, accuracy is better at upper levels. As expected, comparisons with the entire profile of the collocated RAOBs indicate that clear-air water vapor winds represent deeper layers than do either infrared or water vapor cloud-tracked winds. This is because in cloud-free regions the signal from water vapor features may result from emittance over a thicker layer. To further investigate characteristics of the clear-air water vapor winds, they are stratified into two categories that are dependent on the depth of the layer represented by the vector. It is found that if the vertical gradient of moisture is smooth and uniform from near the height assignment upwards, the clear-air water vapor wind tends to represent a relatively deep layer. The information from the comparisons is then used in numerical model simulations of two separate events to determine the forecast impacts. Four simulations are performed for each case: 1) A control simulation that assimilates no satellite wind data, 2) assimilation of all GOES winds according to their assigned single level height, 3) assimilation of all GOES winds spread over multiple levels, and 4) assimilation of all GOES winds spread over multiple levels, but with variations in the vertical influence of clear-air water vapor winds based on the moisture profile in the model. In the first case, a strong mid-latitude cyclone is present and the use of the satellite data results in improved storm tracks during the initial approx. 36 h forecast period. This is because the satellite data improves the analysis of the environment into which the storm progresses. Statistics for mean wind vector and height differences show that, with the exception of the height field at later times in the first case, the use of GOES winds improves the simulation with time. The simulation results suggest that it is beneficial to spread the GOES wind information over multiple levels, particularly when the moisture profile is used to define the vertical influence.

Rao, P. Anil↗