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The transfer of commodities, software, or technlogies to foreign persons is subject to U.S. export control laws and regulations. These export controls are applicable, regardless of whether the transfer occurs in the U.S. or outside of the U.S.
Export Control Reform has been in motion for six years. In 2014, the ITAR regulations surrendered export control of nuclear weapons technology to the control of DOE. Yet no Federal Register notice by DOE detailed where those new regulations could be found. This article explains the disposition that occurred.
The goal of this work is to assess the potential for US export of hydrogen. This covers export market potential, technical production potential and the ability to leverage existing liquefied natural gas (LNG) infrastructure.
A paired-watershed approach was used to compare the quality and fluxes of dissolved organic matter (DOM) during stormflow and baseflow in two lowland tropical rainforest streams located in northeastern Costa Rica. The Arboleda stream received regional groundwater (RGW) flow, whereas the Taconazo stream did not. DOM quality was assessed with absorbance and fluorescence and stable carbon isotope (δ 13 C-DOC) values. RGW DOM lacked detectable fluorescence and had specific ultraviolet absorption (SUVA 254 ) and absorbance slope ratio (S R ) values consistent with low aromaticity and low molecular weight material, respectively. We attributed these properties to microbial degradation and sorption of humic DOM to mineral surfaces during transport through bedrock. SUVA 254 values were lower and SR values were higher in the Arboleda stream during baseflow compared to the Taconazo stream, presumably due to dilution by RGW. However, no significant difference in SUVA 254 or SR occurred between the streams during stormflow. SUVA254 was negatively correlated to δ13C-DOC (r 2 = 0.61, P < 0.001), demonstrating a strong linkage between stream DOM characteristics and the relative amounts of RGW flow and local watershed runoff containing soil and throughfall C sources. Mean DOC export from the Taconazo stream during the study period was 2.62 ± 0.39 g C m -2 year -1 -2 year -1 , one of the highest exports reported and demonstrating a substantial impact of old RGW from outside the watershed boundary can have on surface water carbon cycling.
Gene expression is regulated at multiple steps, such as transcription, splicing, export, degradation and translation. Considering diverse roles of SR proteins, we determined whether the tumor-related splicing factor SRSF3 regulates the expression of the tumor-suppressor protein, PDCD4, at multiple steps. As we have reported previously, knockdown of SRSF3 increased the PDCD4 protein level in SW480 colon cancer cells. More interestingly, here we showed that the alternative splicing and the nuclear export of minor isoforms of pdcd4 mRNA were repressed by SRSF3, but the translation step was unaffected. In contrast, only the translation step of the major isoform of pdcd4 mRNA was repressed by SRSF3. Therefore, overexpression of SRSF3 might be relevant to the repression of all isoforms of PDCD4 protein levels in most types of cancer cell. We propose that SRSF3 could act as a coordinator of the expression of PDCD4 protein via two mechanisms on two alternatively spliced mRNA isoforms.
Here, hexavalent chromium (Cr(VI)) is generated in serpentine soils and exported to surface and groundwaters at levels above health-based drinking water standards. Although Cr(VI) concentrations are elevated in serpentine soil pore water, few studies have reported field evidence documenting Cr(VI) production rates and fluxes that govern Cr(VI) transport from soil to water sources. We report Cr speciation (i) in four serpentine soil depth profiles derived from the California Coast Range serpentinite belt and (ii) in local surface waters. Within soils, we detected Cr(VI) in the same horizons where Cr(III)-minerals are colocated with biogenic Mn(III/IV)-oxides, suggesting Cr(VI) generation through oxidation by Mn-oxides. Water-extractable Cr(VI) concentrations increase with depth constituting a 7.8 to 12 kg/km 2 reservoir of Cr(VI) in soil. Here, Cr(VI) is produced at a rate of 0.3 to 4.8 kg Cr(VI)/km 2 /yr and subsequently flushed from soil during water infiltration, exporting 0.01 to 3.9 kg Cr(VI)/km 2 /yr at concentrations ranging from 25 to 172 μg/L. Although soil-derived Cr(VI) is leached from soil at concentrations exceeding 10 μg/L, due to reduction and dilution during transport to streams, Cr(VI) levels measured in local surface waters largely remain below California’s drinking water limit.
The ATP-binding cassette (ABC) transporter of mitochondria (Atm1) mediates iron homeostasis in eukaryotes, while the prokaryotic homolog from Novosphingobium aromaticivorans (NaAtm1) can export glutathione derivatives and confer protection against heavy-metal toxicity. To establish the structural framework underlying the NaAtm1 transport mechanism, we determined eight structures by X-ray crystallography and single-particle cryo-electron microscopy in distinct conformational states, stabilized by individual disulfide crosslinks and nucleotides. As NaAtm1 progresses through the transport cycle, conformational changes in transmembrane helix 6 (TM6) alter the glutathione-binding site and the associated substrate-binding cavity. Significantly, kinking of TM6 in the post-ATP hydrolysis state stabilized by MgADPVO4 eliminates this cavity, precluding uptake of glutathione derivatives. The presence of this cavity during the transition from the inward-facing to outward-facing conformational states, and its absence in the reverse direction, thereby provide an elegant and conceptually simple mechanism for enforcing the export directionality of transport by NaAtm1. One of the disulfide crosslinked NaAtm1 variants characterized in this work retains significant glutathione transport activity, suggesting that ATP hydrolysis and substrate transport by Atm1 may involve a limited set of conformational states with minimal separation of the nucleotide-binding domains in the inward-facing conformation.
Sucrose translocation from photosynthetic leaves to distant parts of a plant, such as seeds and roots, is a critical aspect of plant growth and development and a major determinant of crop yield. To identify genes contributing to this process in maize (Zea mays), we isolated four allelic mutants, carbohydrate partitioning defective7, 48, 49 (cpd7, cpd48, cpd49) and a UniformMu insertion (mu1049954), all of which exhibited reduced growth and fertility and hyperaccumulation of starch and soluble sugars in mature leaves. Consistent with carbohydrate accumulation, cpd7 mutants exhibited reduced sucrose export from mature leaves. Cpd7 encodes a Golgi-resident glucuronosyltransferase belonging to the Glycosyltransferase14 (GT14) family, which is involved in decoration of type II arabinogalactan proteins. No previously described GT14 mutants exhibit reduced sucrose transport or carbohydrate partitioning defects. Additionally, we show that mature leaves of cpd7 mutants have reduced cellulose content and an altered cell wall composition. Further, cpd7 mutants exhibit ectopic phloem lignification likely as a compensatory mechanism for reduced cell wall integrity. Collectively, our data suggest that Cpd7 functions to facilitate cell wall development in the phloem, which is required for efficient sucrose export from mature maize leaves.
The crystal structures of the individual domains of the Mex67–Mtr2 complex from C. thermophilum have been determined and their arrangement in solution has been studied by SAXS. Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67–Mtr2 that facilitate mRNA nuclear export.
nexport is a lightweight python 3 package which enables neural network developers to export the weights and biases of trained networks to useful file types. It provides I/O support for applications which require specific data types for instantiating neural networks in various inference environments. It was initially developed to support exporting to the data structure used by 'inference-engine', a Fortran inference package.
Meltwater from Greenland is an important freshwater source for the North Atlantic Ocean, released into the ocean at the head of fjords in the form of runoff, submarine melt, and icebergs. The meltwater release gives rise to complex in-fjord transformations that result in its dilution through mixing with other water masses. The transformed waters, which contain the meltwater, are exported from the fjords as a new water mass Glacially Modified Water (GMW). Here we use summer hydrographic data collected from 2013 to 2019 in Upernavik, a major glacial fjord in northwest Greenland, to describe the water masses that flow into the fjord from the shelf and the exported GMWs. Using an optimum multi-parameter technique across multiple years we then show that GMW is composed of 57.8% ± 8.1% Atlantic Water (AW), 41.0% ± 8.3% Polar Water (PW), 1.0% ± 0.1% subglacial discharge, and 0.2% ± 0.2% submarine meltwater. We show that the GMW fractional composition cannot be described by buoyant plume theory alone since it includes lateral mixing within the upper layers of the fjord not accounted for by buoyant plume dynamics. Consistent with its composition, we find that changes in GMW properties reflect changes in the AW and PW source waters. Using the obtained dilution ratios, this study suggests that the exchange across the fjord mouth during summer is on the order of 50 mSv (1 Sv ≡ 10 6 m 3 s –1 ) (compared to a freshwater input of 0.5 mSv). This study provides a first-order parameterization for the exchange at the mouth of glacial fjords for large-scale ocean models.
This dataset is associated with the publication “Disinfection byproducts formed during drinking water treatment reveal an export control point for dissolved organic matter in a subalpine headwater stream” published in Water Research X (Leonard et al. 2022; https://doi.org/10.1016/j.wroa.2022.100144). The associated study analyzed temporal trends from the Town of Crested Butte water treatment facility and synoptic sampling at Coal Creek in Crested Butte, Colorado, US. This work demonstrates how drinking water quality archives combined with synoptic sampling and targeted analyses can be used to identify and understand export control points for dissolved organic matter. This dataset is comprised of one main data folder containing (1) file-level metadata; (2) data dictionary; (3) metadata and international geo-sample number (IGSN) mapping file; (4) dissolved organic carbon (DOC), ultraviolet absorbance at 254 nanometers (UV254), total nitrogen (TN), and specific ultraviolet absorbance (SUVA) data; (5) disinfection bioproduct formation potential (DBP-FP) data; (6) readme; (7) methods codes; (8) water collection protocol; (9) folder of high resolution characterization of organic matter via 12 Tesla Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) through the Environmental Molecular Sciences Laboratory (EMSL; https://www.pnnl.gov/environmental-molecular-sciences-laboratory); and (10) folder of excitation emissions matrix (EEM) spectra. The FTICR folder contains a file of DOC (measured as non-purgeable organic carbon; NPOC) used for FTICR sample preparation. The FTICR folder also contains three subfolders: one subfolder containing the raw .xml data files, one containing the processed data, and the other containing instructions for using Formularity (https://omics.pnl.gov/software/formularity) and an R script to process the data based on the user's specific needs. All files are .csv, .pdf, .dat, .R, .ref, or .xml
The scope of the project is to investigate efflux pump systems in engineered host microorganisms, such as E. coli, and develop a pump engineered to export a target compound. To initiate the project in coordination with other TOTAL driven projects, the first target compound to be studied was 1-hexene. However, we were investigating other chemicals as Styrene. The main goal of the project was to generate a set of optimized efflux pump systems for microorganisms (E. coli and Streptomyces or other host) engineered to contain biosynthetic pathways to export large titers of target compounds that are toxic (or accumulate and push back biosynthesis) to the host cell. An optimized microbial host will utilize specific and efficient cell wall located pumps to extrude harmful target compounds and enable greater production of these compounds.
The purpose of this study was to compare environmental implications of exporting United States (U.S.) coal resources to Asian markets with respect to alternative global sources of steam coal. This study seeks to evaluate and understand potential environmental consequences of exporting PRB coal compared to global alternative sources of coal. This study was informed by a 10-person industry based Technical Steering Committee to improve the quality of the analysis. The key question addressed by the study: Is there a definitive difference between the life cycle GHG profiles between sourcing coal from the U.S. (PRB), Australia, or Indonesia for Japan, South Korea, or Taiwan? Given the uncertainty in the model parameter values, there is not a definitive difference between the life cycle GHG profiles between sourcing coal from the U.S. (PRB), Australia, or Indonesia for Japan, South Korea, or Taiwan.
This study seeks to evaluate and understand potential environmental consequences of exporting Powder River Basin (PRB) coal compared to global alternative sources of coal. Some of the questions that arise in regards to environmental impacts of PRB exports to Asia include the following. (1) Which stages of the life cycle (e.g., mining, transport, power plant combustion) contribute the most to environmental impacts? (2) How do environmental impacts at each stage differ between the PRB and competing countries? (3) Do environmental impacts differ substantially based on the importing country? (4) Is there a definitive difference between the life cycle greenhouse gas (GHG) profiles between sourcing coal from the U.S. (PRB), Australia, or Indonesia for Japan, South Korea, or Taiwan?
This analysis is an update to the 2014 analysis. It calculates the life cycle greenhouse gas (GHG) emissions from liquefied natural gas (LNG) exported from the U.S. and combusted by power plants in Europe or Asia and compares them to regional coal combusted by power plants in Europe and Asia. This analysis also calculates the GHG emissions from natural gas that is extracted in Russia and delivered by pipeline to European and Asian power plants. This analysis is based on data that were originally developed to represent U.S. energy systems: foreign natural gas and coal production were modeled as represented by U.S. natural gas production and average U.S. coal production. The results show that the use of U.S. LNG exports for power production in European and Asian markets will not increase GHG emissions, on a life cycle perspective, when compared to regional coal extraction and consumption for power production.
Biogeochemical cycles within mountainous watersheds are key regulators of ecosystem carbon storage and downstream nutrient loadings, and they have shown to be particularly vulnerable to climate change impacts. Increasing temperature and persistent droughts have already dramatically changed vegetation cover across the mountainous western US, with unknown consequences for soil carbon and nutrient cycles belowground. What remains elusive is to what extent associated changes in root-soil interactions may mobilize the vast pool of organic matter (OM) that has been stabilized by associations with minerals for centuries or millennia. Although plant root-driven OM mobilization from minerals may be a central control on carbon loss and nutrient export, such mechanisms are currently missing from conceptual and numerical models. The overall objective of this Exploratory Project is to identify the biogeochemical mechanisms by which roots destabilize mineral-associated organic matter (MAOM) and the cumulative impact on carbon and nutrient fate. To accomplish this goal, we integrated well-controlled laboratory experiments with in-field measurements and a scalable modeling approach. First, we conducted model system experiments to assess the vulnerability of MAOM to exudate-mediated mechanisms. Our results show that common root exudates effectively destabilize MAOM not only through direct, ligand-driven mobilization mechanisms, but also indirect, microbially-mediated mechanisms relying on secondary metabolites and enzymes. We further found that OM bound to poorly crystalline Fe and Al (hydr)oxides is more vulnerable to exudate-induced destabilization than OM bound to more crystalline phases, particularly in response to direct, ligand-promoted mechanisms. These findings demonstrate that the stability of MAOM is not just a function of their inherent properties, but also will depend in large parts on the ability of plant roots and microbes to produce exudates capable of triggering suitable mobilization mechanisms. We further employed a well-controlled rhizobox approach, combining advanced microsensor and mass spectrometry techniques, to resolve spatiotemporal variations in the composition and availability of exudates along single growing roots of grasses. Our results show that the composition of functionally relevant exudate compounds varies at extremely short time scales, seemingly shifting from ligands such as aromatic acids around root tips to less reactive metabolites such as amino acids around mature root segments. These results suggest a prevalence of direct MAOM mobilization mechanisms around the root tip, while indirect MAOM mobilization strategies may dominate around more mature root segments. Additionally, we utilized microsensor measurements in rhizoboxes to parameterize a rhizosphere (hydro)biogeochemistry reactive transport model (eSTOMP-ROOTS). The resulting model was used to assess how root exudations affects the stability of MAOM. Model simulations show that diel pulses of root exudation are strong enough to cause oscillations in biogeochemical conditions—particularly in pH, oxygen concentrations, and microbial activity— that repeatedly disrupt MAOM. Finally, we assessed field-scale impacts of root-driven MAOM destabilization over short and long time scales. A combination of in-field incubations and field-based characterizations showed that MAOM may not only be vulnerable to seasonal variations in root activity, but also to root-driven weathering over pedogenic time scales. In sum, results from our Exploratory Award highlight the strong control plant roots exert on MAOM mobilization and, thus, on the potential for carbon and nutrient export from watersheds.
To demonstrate and validate the performance of the wide-area damping control system, the project plans to conduct closed-loop tests on the PDCI in spring/summer 2017. A test plan details the open and closed loop tests to be conducted on the PDCI using the wide-area damping control system. To ensure the appropriate level of preparedness, simulations were performed in order to predict and evaluate any possible unsafe operations before hardware experiments are attempted. This report contains the results from these simulations using the power system dynamics software PSLF (Power System Load Flow, trademark of GE). The simulations use the WECC (Western Electricity Coordinating Council) 2016 light summer and heavy summer base cases and the 2014 dual export base case. Because of the large volume of plots, the results were divided into three reports corresponding to the three base cases. This report contains results from the 2014 dual export base case.