Current problems in the cultivation of Hydrogenomonas bacteria under controlled environments in the turbidostat
Environment effects on growth of Hydrogenomonas eutropha in dense culture
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Environment effects on growth of Hydrogenomonas eutropha in dense culture
Bone mineral changes in prolonged bed rest subjects determined by gamma ray absorption technique
The primary objective of this research is to obtain fundamental data concerning the growth and metabolism of the unicellular green algae. These organisms are most likely to provide biological oxygen and a food source for space crews. Biochemical conversions, chemical composition, and cell growth and division are discussed. Chlorella sorokiniana is emphasized.
An anesthetic gas flow pop-off valve canister is described that is airtight and permits the patient to breath freely. Once its release mechanism is activated, the exhaust gases are collected at a hose adapter and passed through activated coal for adsorption. A survey of laminar air flow clean rooms is presented and the installation of laminar cross flow air systems in operating rooms is recommended. Laminar flow ventilation experiments determine drying period evaporation rates for chicken intestines, sponges, and sections of pig stomach.
In an attempt to understand the responses of CHLORELLA to various quantities and qualities of light in space flight life support system, studies were designed to give the maximum rates of growth as well as maximum yields at different densities of algae under different light intensities, and under light of different wave lengths. The results of studies on the effects of light on algal growth revealed that the effect was not only positive, as had been assumed in the case of photosynthesis, but that light had a negative action also. Light at the blue end of the spectrum was clearly inhibitory to cell division and vegetative reproduction. Carbon dioxide also limited growth by inhibition of cell divisions in CHLORELLA as well as in the colorless yeast SACCHAROMYCES.
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Theoretical development and computer implementation of three guidance algorithms are presented. From a small set of input parameters the algorithms generate the ground track, altitude profile, and speed profile required to implement an experimental 4-D guidance system. Given a sequence of waypoints that define a nominal flight path, the first algorithm generates a realistic, flyable ground track consisting of a sequence of straight line segments and circular arcs. Each circular turn is constrained by the minimum turning radius of the aircraft. The ground track and the specified waypoint altitudes are used as inputs to the second algorithm which generates the altitude profile. The altitude profile consists of piecewise constant flight path angle segments, each segment lying within specified upper and lower bounds. The third algorithm generates a feasible speed profile subject to constraints on the rate of change in speed, permissible speed ranges, and effects of wind. Flight path parameters are then combined into a chronological sequence to form the 4-D guidance vectors. These vectors can be used to drive the autopilot/autothrottle of the aircraft so that a 4-D flight path could be tracked completely automatically; or these vectors may be used to drive the flight director and other cockpit displays, thereby enabling the pilot to track a 4-D flight path manually.
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The Ribbon-to-Ribbon (RTR) process for growth of silicon ribbon is described. This process involves the fabrication of a microcrystalline ribbon of silicon and subsequent grain size enhancement through a laser recrystallization process. The microribbon is obtained from a Thermal Expansion Shear Separation (TESS) process which allows a CVD layer of silicon to be separated from a temporary molybdenum substrate. Efforts to achieve increased solar cell efficiencies and higher area production rates have been problematical. Furnaces, which are necessary for thermal stress control, have been shown to contribute contamination to substrates resulting in degraded efficiencies. Recent results with a new furnace design indicate efficiencies in excess of 9% will be routine. Limitations to area throughput arise due to fundamental linear velocity limitations and width limitations necessary to prevent the occurrence of thermal buckling. Calculations are reported which show the influence of thermal profile on buckling tendencies, and a proposed electron beam technique is considered which promises high throughput with minimal buckling.
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Emphasis was given to environmental factors that alter the flux of carbohydrate from the shoot to the root system to support the absorption of nitrogen and the subsequent interaction between nitrogen uptake and whole plant growth. Only the nitrate form of nitrogen was utilized in order to establish base line responses for evaluation of future studies utilizing both ammonium and nitrate.
A research project in the food production group of the Closed Ecological Life Support System (CELSS) program sought to define optimum conditions for photosynthetic productivity of a higher plant food crop. The effects of radiation and various atmospheric compositions were studied.
Utilizing high-frequency data from a highly instrumented rotor assembly, seeded bearing defect signatures are characterized using both conventional linear approaches, such as power spectral density analysis, and recently developed nonlinear techniques such as bicoherence analysis. Traditional low-frequency (less than 20 kHz) analysis and high-frequency envelope analysis of both accelerometer and acoustic emission data are used to recover characteristic bearing distress information buried deeply in acquired data. The successful coupling of newly developed nonlinear signal analysis with recovered wideband envelope data from accelerometers and acoustic emission sensors is the innovative focus of this research.
The widespread application of electric (often called artificial) light in greenhouses, growing rooms, and plant growth chambers would presuppose that the role of phytochrome would be considered in the selection and use of such lighting systems. Unfortunately this is not usually the case. Part of the problem is that many students, and indeed an unfortunate number of senior scientists, seem to regard phytochrome as a laboratory phenomenon without much application in the real world. They simply have not grasped the concept that phytochrome is functioning through all stages of plant development, wherever plants are grown. It is certainly true, as Meijer (1971) stated, that one cannot compare experimental results obtained under very strict laboratory conditions with plant irradiation in glasshouses and in growth rooms. When Karl Norris developed the first practical portable spectroradiometer about 1962, some of the first measurements were to determine the red/far-red ratios under tree canopies. These measurements showed clearly the predominance of far-red in the understory and suggested that far-red was contributing to the elongation exhibited by many species growing in the shade, and possibly was a factor in the induction of light requirements in seeds. Subsequently we used Catalpa leaves as far-red filters to make light-insensitive lettuce seed light requiring. Much more detailed work has since been done on phytochrome effects in the natural environment, and it is encouraging to note that efforts are bring made to apply phytochrome research to horticulture.
These guidelines for lighting of plants, established by the organizing committee, provided a focus for the discussions at the workshop. The guidelines served as a basis for discussion amongst the workshop attendees and led to a number of recommendations that were recorded by the session chairpersons. The organizing committee indicated they would incorporate the recommendations and suggestions into a revised set of guidelines for additional discussion. It was understood that these guidelines will not be standards and will require upgrading and modifications as lamps and equipment become available and as new insights are obtained on plants' response to light. Revised draft guidelines are included as Tables 1 and 2 that have been developed by the organizing committee following the suggestions obtained at the workshop. The guidelines for growth chambers are in Table 1 and guideines for greenhouses are given in Table 2. These have been distributed to the participants who indicated a willingness to review proposals that were developed. It is hoped that these proposals will lead to the development of guidelines that will have general acceptance by plant scientists.
Volatile organic compound(VOC), ethylene gas, was characterized and quantified by GC/FID. 20-50 ppb levels were detected during the growth stages of radish. SPME could be a good analytical tool for the purpose. Low temperature trapping method using dry ice/diethyl ether and liquid nitrogen bath was recommended for the sampling process for GC/PID and GC/MS analysis.
Sweetpotato is one of several crops recommended by National Aeronautics and Space Administration (NASA) for bioregenerative life support studies. One of the objectives of the Tuskegee University NASA Center is to optimize growth conditions for adaptability of sweetpotatoes for closed bioregenerative systems. The role of nutrient solution management as it impacts yield has been one of the major thrusts in these studies. Nutrient solution management protocol currently used consists of a modified half Hoagland solution that is changed at 14-day intervals. Reservoirs are refilled with deionized water if the volume of the nutrient solution was reduced to 8 liters or less before the time of solution change. There is the need to recycle and replenish nutrient solution during crop growth, rather than discard at 14 day intervals as previously done, in order to reduce waste. Experiments were conducted in an environmental growth room to examine the effects of container size on the growth of several sweetpotato genotypes grown under a nutrient replenishment protocol. Plants were grown from vine cuttings of 15cm length and were planted in 0.15 x 0.15 x 1.2m growth channels using a closed nutrient film technique system. Nutrient was supplied in a modified half strength Hoagland's solution with a 1:2.4 N:K ratio. Nutrient replenishment protocol consisted of daily water replenishment to a constant volume of 30.4 liters in the small containers and 273.6 liters in the large container. Nutrients were replenished as needed when the EC of the nutrient solution fell below 1200 mhos/cm. The experimental design used was a split-plot with the main plot being container size and genotypes as the subplot. Nine sweetpotato genotypes were evaluated. Results showed no effect of nutrient solution container size on storage root yield, foliage fresh and dry mass, leaf area or vine length. However, plants grown using the large nutrient solution container accumulated more storage root dry mass than those with the small containers. Although plants grown with the smaller containers showed greater water uptake, plant nutrient uptake was lower than with the larger container. All genotypes evaluated showed variation in their responses to all parameters measured.
Sweetpotato was grown to harvest maturity within NASA Johnson Space Center's Variable Pressure Growth Chamber (VPGC) to characterize crop performance for potential use in advanced life support systems as a contributor to food production, air revitalization and resource recovery. Stem cuttings of breeding clone "TU-82-155" were grown hydroponically at a density of 17 plants m(sup -2) using a modified pressure-plate growing system (Patent No. 4860-490, Tuskegee University). Lighting was provided by HPS lamps at a photoperiod of 12h light: 12h dark. The photosynthetic photon flux was maintained at 500, 750 and 1000 micro mol m(sup -2) s(sub -1) during days 1-15, 16-28, 29-119, respectively. Canopy temperatures were maintained at 28 C: light: 22 C:dark. During the light period, relative humidity and carbon dioxide were maintained at 70% and 1200 micro liters l(sup -1), respectively. Nutrient solution was manually adjusted 2 to 4 times per week by addition of 10X concentrated modified half-strength Hoagland nutrient salts and NaOH to return the electrical conductivity and pH to 1.2 mS cm(sup -1) and 6.0, respectively. At 17 weeks (119 days) from transplanting, a total of 56.5 kilograms fresh mass of storage roots (84.1% moisture) were harvested from the 11.2 m(sup 2) chamber, resulting in a yield 5.0 kilograms m(sup -2). Harvest index, based on fresh mass, was 38.6%. Rates of net photosynthesis, dark respiration, transpiration, and ethylene production will be reported.