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At least 91 records · Page 5

A method for achieving complete microbial genomes and improving bins from metagenomics data

Metagenomics facilitates the study of the genetic information from uncultured microbes and complex microbial communities. Assembling complete microbial genomes ( i.e ., circular with no misassemblies) from metagenomics data is difficult because most samples have high organismal complexity and strain diversity. Less than 100 circularized bacterial and archaeal genomes have been assembled from metagenomics data despite the thousands of datasets that are available. Circularized genomes are important for (1) building a reference collection as scaffolds for future assemblies, (2) providing complete gene content of a genome, (3) confirming little or no contamination of a genome, (4) studying the genomic context and synteny of genes, and (5) linking protein coding genes to ribosomal RNA genes to aid metabolic inference in 16S rRNA gene sequencing studies. We developed a method to achieve circularized genomes using iterative assembly, binning, and read mapping. In addition, this method exposes potential misassemblies from k-mer based assemblies. We chose species of the Candidate Phyla Radiation (CPR) to focus our initial efforts because they have small genomes and are only known to have one ribosomal RNA operon. We present 34 circular CPR genomes, one circular Margulisbacteria genome, and two circular megaphage genomes from 19 public and published datasets. We demonstrate findings that would likely be difficult without circularizing genomes, including that ribosomal genes are likely not operonic in the majority of CPR, and that some CPR harbor diverged forms of RNase P RNA. Code and a tutorial for this method is available at https://github.com/lmlui/Jorg .

Lui, Lauren↗

First M87 Event Horizon Telescope Results. IX. Detection of Near-horizon Circular Polarization

Event Horizon Telescope (EHT) observations have revealed a bright ring of emission around the supermassive black hole at the center of the M87 galaxy. EHT images in linear polarization have further identified a coherent spiral pattern around the black hole, produced from ordered magnetic fields threading the emitting plasma. Here we present the first analysis of circular polarization using EHT data, acquired in 2017, which can potentially provide additional insights into the magnetic fields and plasma composition near the black hole. Interferometric closure quantities provide convincing evidence for the presence of circularly polarized emission on event-horizon scales. We produce images of the circular polarization using both traditional and newly developed methods. All methods find a moderate level of resolved circular polarization across the image ( $\langle$| v |$\rangle$ < 3.7%), consistent with the low image-integrated circular polarization fraction measured by the Atacama Large Millimeter/submillimeter Array (| v int |< 1%). Despite this broad agreement, the methods show substantial variation in the morphology of the circularly polarized emission, indicating that our conclusions are strongly dependent on the imaging assumptions because of the limited baseline coverage, uncertain telescope gain calibration, and weakly polarized signal. We include this upper limit in an updated comparison to general relativistic magnetohydrodynamic simulation models. This analysis reinforces the previously reported preference for magnetically arrested accretion flow models. We find that most simulations naturally produce a low level of circular polarization consistent with our upper limit and that Faraday conversion is likely the dominant production mechanism for circular polarization at 230 GHz in M87*.

79 ASTRONOMY AND ASTROPHYSICS↗

Orbit Determination for Mars Global Surveyor During Mapping

The Mars Global Surveyor (MGS) spacecraft reached a low-altitude circular orbit on February 4, 1999, after the termination of the second phase of aerobraking. The MGS spacecraft carries the Mars Orbiter Laser Altimeter (MOLA) whose primary goal is to derive a global, geodetically referenced 0.2 deg x 0.2 deg topographic grid of Mars with a vertical accuracy of better than 30 meters. During the interim science orbits in the' Hiatus mission phase (October - November 1997), and the Science Phasing Orbits (March - April, 1998, and June - July 1998) 208 passes of altimeter data were collected by the MOLA instrument. On March 1, 1999 the first ten orbits of MOLA altimeter data from the near-circular orbit were successfully returned from MGS by the Deep Space Network (DSN). Data will be collected from MOLA throughout the Mapping phase of the MCS mission, or for at least one Mars year (687 days). Whereas the interim orbits of Hiatus and SPO were highly eccentric, and altimeter data were only collected near periapsis when the spacecraft was below 785 km, the Mapping orbit of MGS is near circular, and altimeter data will be collected continuously at a rate of 10 Hz. The proper analysis of the altimeter data requires that the orbit of the MGS spacecraft be known to an accuracy comparable to that of the quality of the altimeter data. The altimeter has an ultimate precision of 30 cm on mostly flat surfaces, so ideally the orbits of the MGS spacecraft should be known to this level. This is a stringent requirement, and more realistic goals of orbit error for MGS are ten to thirty meters. In this paper we will discuss the force and measurement modelling required to achieve this objective. Issues in force modelling include the proper modelling of the gravity field of Mars, and the modelling of non-conservatives forces, including the development of a 'macro-model', in a similar fashion to TOPEX/POSEIDON and TDRSS. During Cruise and Aerobraking, the high gain antenna (HGA) was stowed on the +X face of the spacecraft. On March 29, 1999 the HGA will be deployed on a meter long boom which will remain Earth-pointing while the instrument panel (including the MOLA instrument) remains pointed at nadir. The tracking data must be corrected for the regular motion of the high gain antenna with respect to the center of mass, and the success of the MGS determination during Mapping will depend on correctly accounting for this offset in the measurement model.

Lemoine, F. G.↗

A circular median filter approach for resolving directional ambiguities in wind fields retrieved from spaceborne scatterometer data

The retrieval algorithm for spaceborne scatterometry proposed by Schultz (1985) is extended. A circular median filter (CMF) method is presented, which operates on wind directions independently of wind speed, removing any implicit wind speed dependence. A cell weighting scheme is included in the algorithm, permitting greater weights to be assigned to more reliable data. The mathematical properties of the ambiguous solutions to the wind retrieval problem are reviewed. The CMF algorithm is tested on twelve simulated data sets. The effects of spatially correlated likelihood assignment errors on the performance of the CMF algorithm are examined. Also, consideration is given to a wind field smoothing technique that uses a CMF.

Schultz, Howard↗

Time variation in the reaction-zone structure of two-phase spray detonations.

A detailed theoretical analysis of the time-varying detonation structure in a monodisperse spray is presented. The theory identifies experimentally observed reaction-zone overpressures as deriving from blast waves formed therein by the explosive ignition of the spray droplets, and follows in time the motion, change in strength, and interactions of these blast waves with one another, and with the leading shock. The results are compared with experimental data by modeling the motion of a finite-size circular pressure transducer through the theoretical data field in an x-t space.

Pierce, T. H.↗

Laser velocimeter and total pressure measurements in circular-to-rectangular transition ducts

A comprehensive set of total pressure and three-component laser velocimetry (LV) data were obtained within two circular-to-rectangular transition ducts at low subsonic speeds. This set of reference data was acquired for use in identifying secondary flow mechanisms and for assessing the accuracy of computational procedures for calculating such flows. Data were obtained at the inlet and exit planes of an aspect ratio three duct having a length-to-diameter ratio of one (AR310) and an aspect ratio six duct having a length-to-diameter ratio of three (AR630). Each duct was unseparated throughout its transition section. It is therefore concluded that secondary flows can play an important part in the fluid dynamics of transition ducts and needs to be addressed in computational analysis. The strength of the secondary flows depends on both the aspect ratio and relative axial duct length.

Patrick, William P.↗

Previously Unrecognized Large Lunar Impact Basins Revealed by Topographic Data

The discovery of a large population of apparently buried impact craters on Mars, revealed as Quasi- Circular Depressions (QCDs) in Mars Orbiting Laser Altimeter (MOLA) data [1,2,3] and as Circular Thin Areas (CTAs) [4] in crustal thickness model data [5] leads to the obvious question: are there unrecognized impact features on the Moon and other bodies in the solar system? Early analysis of Clementine topography revealed several large impact basins not previously known [6,7], so the answer certainly is "Yes." How large a population of previously undetected impact basins, their size frequency distribution, and how much these added craters and basins will change ideas about the early cratering history and Late Heavy Bombardment on the Moon remains to be determined. Lunar Orbiter Laser Altimeter (LOLA) data [8] will be able to address these issues. As a prelude, we searched the state-of-the-art global topographic grid for the Moon, the Unified Lunar Control Net (ULCN) [9] for evidence of large impact features not previously recognized by photogeologic mapping, as summarized by Wilhelms [lo].

Frey, Herbert V.↗

Very accurate upward continuation to low heights in a test of non-Newtonian theory

Recently, gravity measurements were made on a tall, very stable television transmitting tower in order to detect a non-Newtonian gravitational force. This experiment required the upward continuation of gravity from the Earth's surface to points as high as only 600 m above ground. The upward continuation was based on a set of gravity anomalies in the vicinity of the tower whose data distribution exhibits essential circular symmetry and appropriate radial attenuation. Two methods were applied to perform the upward continuation - least-squares solution of a local harmonic expansion and least-squares collocation. Both methods yield comparable results, and have estimated accuracies on the order of 50 microGal or better (1 microGal = 10(exp -8) m/sq s). This order of accuracy is commensurate with the tower gravity measurments (which have an estimated accuracy of 20 microGal), and enabled a definitive detection of non-Newtonian gravity. As expected, such precise upward continuations require very dense data near the tower. Less expected was the requirement of data (though sparse) up to 220 km away from the tower (in the case that only an ellipsoidal reference gravity is applied).

Romaides, Anestis J.↗

(abstract) Venus Gravity Data Reduction

The Magellan spacecraft has provided high resolution gravity data to its very end, October 13, 1994, when it was consumed by the Venusian atmosphere. After aerobraking in August of 1993 to attain a near circular orbit, excellent high latitude data were acquired which previously were very weak during the elliptical orbit coverage. There are 1500 orbits during the near circular orbit, supplying redundant coverage at different geometries over many features. This allowed the relaxation of apriori constraints, so true amplitudes are being extracted from the data. In this paper we present the results of a 75(sup th) degree and order field that incorporates all the old Pioneer Venus Orbiter data as well as all the Magellan data to September 1994. The new results reflect even higher correlation with topography, higher amplitude values for the highs and lows, and global results that have essentially very little apriori constraint on the solution parameters. We also correlate our new model with the earlier ones based on 60(sup th) and 40(sup th) degree and order presentations.

Magellan Venus gravity orbits topography surface f↗

Magellan: Principal Venus science findings

This is a brief summary of the science findings of the Magellan mission, principally based on data from the radar system. Future plans for Magellan include acquisition of high resolution gravity data from a nearly circular orbit and atmospheric drag and occultation experiments. The Magellan science results represent the combined effort of more than 100 Magellan investigators and their students and colleagues. More extensive discussions can be found in the August and October, 1992 issues of the Journal of Geophysical Research, Planets. The Magellan mission's scientific objectives were to provide a global characterization of landforms and tectonic features; to distinguish and understand impact processes; to define and explain erosion, deposition, and chemical processes; and to model the interior density distribution. All but the last objective, which requires new global gravity data, have been accomplished, or we have acquired the data that are required to accomplish them.

Saunders, R. Stephen↗

Discharge coefficient correlations for circular-arc venturi flowmeters at critical /sonic/ flow

Experimental data are analyzed to support theoretical predictions for discharge coefficients in circular-arc venturi flow meters operating in the critical sonic flow regime at throat Reynolds numbers above 150 thousand. The data tend to verify the predicted 0.25% decrease in the discharge coefficient during transition from a laminar to turbulent boundary layer. Four different test gases and three flow measurement facilities were used in the experiments with 17 venturis with throat sizes from 0.15 to 1.37 in. and Beta ratios ranging from 0.014 to 0.25. Recommendations are given as to how the effectiveness of future studies in the field could be improved.

Arnberg, B. T.↗

Average heat-transfer characteristics of a row of circular air jets impinging on a concave surface

A study of the average heat-transfer characteristics of air jets impinging on the concave side of a right-circular semicylinder is reported. Results from existing correlating are compared with each other and with experimental heat-transfer data for a row of circular jets. Two correlations available in the literature are recommended for use in designing cooled turbine vanes and blades.

Livingood, J. N. B.↗

Potential Foldamers Based on an ortho- Terphenyl Amino Acid

We describe the synthesis and characterization of a new class of oligomers built from a terphenyl-based amino acid. Here, these oligomeric amides are of interest because the adoption of specific conformations could potentially be driven by the coordinated formation of inter-residue hydrogen bonds and aromatic interactions. Although high-resolution structural data have proven inaccessible, circular dichroism and nuclear magnetic resonance studies suggest that the new oligomers fold concomitantly with discrete self-association in chloroform. Molecular simulations find no sign of folding as a monomer, and in fact suggest that hydrogen bond patterning is anti-cooperative, inhibiting longer helices.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

First Measurement Using Elliptically Polarized Photons of the Double-Polarization Observable E for γ p → p π 0 and γ p → n π +

We report the measurement of the helicity asymmetry E for the p π 0 and n π + final states using, for the first time, an elliptically polarized photon beam in combination with a longitudinally polarized target at the Crystal Ball experiment at MAMI. The results agree very well with data that were taken with a circularly polarized photon beam, showing that it is possible to simultaneously measure polarization observables that require linearly (e.g., G ) and circularly polarized photons (e.g., E ) and a longitudinally polarized target. The new data cover a photon energy range 270–1400 MeV for the p π 0 final state (230–842 MeV for the n π + final state) and the full range of pion polar angles, θ , providing the most precise measurement of the observable E . A moment analysis gives a clear observation of the p η cusp in the p π 0 final state. Published by the American Physical Society 2024

Physics↗

Development of termination and utilization concepts for flat conductor cables. Volume 3: Cost study comparison, flat versus round conductor cable

A cost study comparing flat conductor cable (FCC) with small-gage wire (SGW) and conventional round conductor cable (RCC) is presented. This study was based on a vehicle wiring system consisting of 110,000 ft of conventional RCC equally divided between AWG sizes 20,22, and 24 using MIL-W-81044-type wire and MIL-C-26500 circular connectors. Basic cost data were developed on a similar-sized commercial jet airplane wiring system on a previous company R&D program in which advanced wiring techniques were carried through equivalent installations on an airplane mockup; and on data developed on typical average bundles during this program. Various cost elements included were engineering labor, operations (manufacturing) labor, material costs, and cost impact on payload. Engineering labor includes design, wiring system integration, wiring diagrams and cable assembly drawings, wire installations, and other related supporting functions such as the electronic data processing for the wiring. Operations labor includes mockup, tooling and production planning, fabrication, assembly, installation, and quality control cost impact on payload is the conversion of wiring system weight variations through use of different wiring concepts to program payload benefits in terms of dollars.

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