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NASA NTRS2010

Proceedings of the 40th Aerospace Mechanisms Symposium

The Aerospace Mechanisms Symposium (AMS) provides a unique forum for those active in the design, production and use of aerospace mechanisms. A major focus is the reporting of problems and solutions associated with the development and flight certification of new mechanisms. Organized by the Mechanisms Education Association, responsibility for hosting the AMS is shared by the National Aeronautics and Space Administration and Lockheed Martin Space Systems Company (LMSSC). Now in its 40th symposium, the AMS continues to be well attended, attracting participants from both the U.S. and abroad. The 40th AMS, hosted by the Kennedy Space Center (KSC) in Cocoa Beach, Florida, was held May 12, 13 and 14, 2010. During these three days, 38 papers were presented. Topics included gimbals and positioning mechanisms, CubeSats, actuators, Mars rovers, and Space Station mechanisms. Hardware displays during the supplier exhibit gave attendees an opportunity to meet with developers of current and future mechanism components. The use of trade names of manufacturers in this publication does not constitute an official endorsement of such products or manufacturers, either expressed or implied, by the National Aeronautics and Space Administration

Littlefield, Alan C.↗
NASA NTRSDate not supplied

Circulating miRNA Signature Predicts and Rescues Spaceflight Associated Health Risks

We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents and humans in response to simulated and real spaceflight and regulates vascular damage by simulated deep space radiation. In previous studies, we identified miRNAs that are predicted to regulate rodent responses to spaceflight in low-Earth orbit. Here we have validated the expression of key spaceflight-associated miRNAs in rodents reacting to simulated spaceflight conditions (exposure to ionizing radiation combined with simulated microgravity) and in astronaut samples from the NASA Twin Study. We demonstrate that this circulating miRNA siganture is conserved between these different species and will serve as an ideal biomarker to monitor increase health risk due to exposure to the space environment. Lastly, to demonstrate their physiological relevance, we have utilized antagomirs to target a subset of miRNAs from the spaceflight associated signature to successfully rescue simulated deep space radiation-mediated damage in human 3D vascular constructs. These results provide strong evidence to further develop a miRNA based countermeasure in astronauts.

Afshin Beheshti↗
NASA NTRS2020

Circulating Mirna Spaceflight Signature Reveals Targets to Mitigate Associated Health Risks

We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents and humans in response to simulated, short-duration, and long-duration spaceflight and regulates vascular damage caused by simulated deep space radiation. In previous studies, we had identified miRNAs that are predicted to regulate rodent responses to spaceflight in low-Earth orbit. Here we have confirmed the expression of these proposed spaceflight associated miRNAs in rodents reacting to simulated spaceflight conditions (exposure to ionizing radiation combined with simulated microgravity) and in astronaut samples from the NASA Twins Study via direct quantification of miRNAs, miRNA sequencing, and inferring miRNA target levels based on single-cell RNA (scRNA-seq) and single-cell chromatin (scATAC-seq) sequencing data. To demonstrate the physiological relevance of key spaceflight associated miRNAs, we utilized antagomirs to inhibit their expression and successfully rescue simulated deep space radiation-mediated damage in human 3D vascular constructs.

Sherina Malkani↗
NASA NTRSDate not supplied

Large Impact Features on Icy Galilean Satellites: Implications for Ice Shell Thickness and Habitability.

Nowhere in the solar system are impact morphologies observed in greater variety than on the icy Galilean satellites. This is very likely a consequence of the structural and thermal state of the crust at the time of impact, and perhaps impact velocity. We have conducted a multi-disciplinary investigation to study how these features formed. The particular combination of geophysical factors and impactor characteristics that is shared by these satellites is likely responsible for these features. We have derived Digital Terrain Models for a number of these features, which have been used as a tool for producing facies maps. Impact features on Ganymede and Callisto are relatable to impact features on Europa, with implications for its ice shell history. We have identified two broad classes of these impact features: (a) Crater forms, which variously include pits, central domes, and crater rims; and (b) Palimpsests (including pene-palimpsests), which all have smooth enclosed central plains, an extensive outer undulating plains unit, concentric arcuate ridges(in the case of pene-palimpsests), and no recognizable rim. The very large, measured volume of the undulating plains unit of the pene-palimpsest Buto, Ganymede, substantially exceeds the amount of target material that could have been both melted and excavated by the impact event itself. Therefore, we conclude that the undulating plains material was derived from a fluid or “slushy” layer in the near-surface target at the time of impact. The recognition of undulating plains as the dominant unit in Memphis and Nidaba, two other palimpsests on Ganymede that we have mapped(which display highly variable crater counts, with Buto being the least cratered),leads us to a working hypothesis that these plains only form where and when the target has substantial near surface fluid or “slush.” By extension, all other impact features on Ganymede and Callisto, such as those with central pits and central domes, formed in targets with no pre-existing, or at most inconsequential, amounts of fluid or “slush.” Supporting this conclusion, modeling by members of our group indicates that the present topography of impact features with central pits and domes can be largely explained by the behavior of ice alone, with no or little contribution from fluids. Our study supports explanations for the formation and final appearance of palimpsests (and pene-palimpsests) that are entirely applicable to this feature class, and sharply distinct from explanations for other impact features (crater forms) on Ganymede and Callisto.

Jeffrey M Moore↗
NASA NTRS1973

System design of the Pioneer Venus spacecraft. Volume 12: International cooperation

A spectrum of plans has been prepared to illustrate the range of practical sharing possibilities available so as to assist Ames Research Center (ARC) and European Space Research Organization (ESRO) in selection of a program meeting mutual goals. Five plans are described showing increased participation by ESRO WITH ascending plan number. Each of these has sharing properties fulfilling particular requirements such as available ESRO budget level, extent of ESRO program responsibility, matching particular ESRO capability, and cost saving to ARC through sharing. All plans apply to orbiter sharing only. A sharing plan based on the model Plan 4 may offer the most attractive division of Pioneer Venus between ARC and ESRO. This plan allows ESRO to bear primary responsibility for the orbiter and to avoid an extensive financial burden. Savings to ARC are commensurate with ARC loss of program control. Duplication of effort is avoided by using orbiter subsystems that are common to the probe bus and orbiter.

Kelly, R. S.↗
NASA NTRS2010

Impact of Damping Uncertainty on SEA Model Response Variance

Statistical Energy Analysis (SEA) is commonly used to predict high-frequency vibroacoustic levels. This statistical approach provides the mean response over an ensemble of random subsystems that share the same gross system properties such as density, size, and damping. Recently, techniques have been developed to predict the ensemble variance as well as the mean response. However these techniques do not account for uncertainties in the system properties. In the present paper uncertainty in the damping loss factor is propagated through SEA to obtain more realistic prediction bounds that account for both ensemble and damping variance. The analysis is performed on a floor-equipped cylindrical test article that resembles an aircraft fuselage. Realistic bounds on the damping loss factor are determined from measurements acquired on the sidewall of the test article. The analysis demonstrates that uncertainties in damping have the potential to significantly impact the mean and variance of the predicted response.

Schiller, Noah↗
NASA NTRS2000

Implementing Journaling in a Linux Shared Disk File System

In computer systems today, speed and responsiveness is often determined by network and storage subsystem performance. Faster, more scalable networking interfaces like Fibre Channel and Gigabit Ethernet provide the scaffolding from which higher performance computer systems implementations may be constructed, but new thinking is required about how machines interact with network-enabled storage devices. In this paper we describe how we implemented journaling in the Global File System (GFS), a shared-disk, cluster file system for Linux. Our previous three papers on GFS at the Mass Storage Symposium discussed our first three GFS implementations, their performance, and the lessons learned. Our fourth paper describes, appropriately enough, the evolution of GFS version 3 to version 4, which supports journaling and recovery from client failures. In addition, GFS scalability tests extending to 8 machines accessing 8 4-disk enclosures were conducted: these tests showed good scaling. We describe the GFS cluster infrastructure, which is necessary for proper recovery from machine and disk failures in a collection of machines sharing disks using GFS. Finally, we discuss the suitability of Linux for handling the big data requirements of supercomputing centers.

Preslan, Kenneth W.↗
NASA NTRSDate not supplied

Aircraft Classification Using Radar from Small Unmanned Aerial Systems for Scalable Traffic Management Emergency Response Operations

This work investigates two machine learning techniques: Support Vector Machine (SVM) and Autoencoders (AE)with SVM layer for classification of radar trajectories as General Aviation (GA), fixed-wing small Unmanned Aerial System (sUAS), or not-an-aircraft using radar data recorded from sUAS. Onboard identification of intruder aircraft type is useful for planning avoidance maneuvers and is necessary to provide autonomous systems to meet or exceed the avoidance capability of a human pilot. Aircraft classification can identify intruder aircraft that are not part of the team and may be violating a Temporary Flight Restriction. Aircraft classification is needed in monitoring an airspace where multiple aircraft are teaming on a shared task. Scalable Traffic Management for Emergency Response Operations (STEReO) is a NASA project aimed at improving disaster response by enabling large scale aircraft operations through the teaming of manned aircraft with sUAS to maximize emergency response resources. To this end, this work uses trajectories and radar derived features to classify aircraft from a multirotor sUAS. The AE + SVM generated the strongest classification overall accuracy of 93.5% using the first 4 seconds of radar track data for tracks that activated the avoidance system. Subsampling the available track data increased the available training data with the maximum aircraft recall of 0.94 achieved using the SVM with 1 second track data.

Chester V. Dolph↗
NASA NTRS1996

Quantifying the Correlation Between Eye-Movement and Perceptual Responses to Moving Plaids

To determine the extent to which the pursuit eye-movement and perceptual systems share a common neural motion-processing pathway, we simultaneously measure the psychophysical and eye-movement responses to drifting plaids in a direction-discrimination task. Correlation between the noise in the eye movements and percepts would suggest a shared neural substrate. Three observers were asked to track a plaid (Type I 90 deg; TF = 4 Hz, SF = 0.6 c/d; direction = -2, 0, or 2 deg), and to respond whether the motion was to the right or left of vertical. The eye-movement direction is computed from the initial 300 ms of saccade-free tracking (near open-loop). On each trial, we use the eye-movement direction to predict the perceptual response. We then compute the probability of successfully predicting the observer's response. We also use SOC analysis as an alternate measure of the correlation. We compare the data from both these analyses to the predictions of two models in which the eye movements and the percept are driven by either a common noisy signal (correlated model) or by two separate noisy signals (uncorrelated model). We also allow for the fact that our measured eye movements are degraded by eye-tracker noise. This noise causes our measured correlation to be lower than the actual biological correlation. The correlations in our data are higher than those predicted by the uncorrelated model, and are similar to the predictions of the correlated model. The proportion of perceptual responses correctly predicted by the eye movements were 0.66, 0.60, 0.73 for the -2, 0, 2 deg stimuli respectively (correlated model 0.65, 0.60, 0.70; uncorrelated model 0.58, 0.51, 0.60).The SOC proportions were 0.71, 0.64, 0.72 (correlated model 0.66, 0.64, 0.69; uncorrelated model 0.5, 0.5, 0.5). These results show that the oculomotor and perceptual system share a performance-limiting noise source and provide strong evidence that a common neural mechanism (perhaps MT or MST) drives both perception and smooth eye movements.

Beutter, Brent R.↗
NASA NTRSDate not supplied

Biological Insights at the Interface of Multiple Arabidopsis Legacy Datasets

The NASA GeneLab database includes an open-access collection of datasets yielded by space biology experiments. Six Gene Lab Data Sets (GLDS’s) performed in Arabidopsis were selected for analysis (7/17/44/121/205/213), all of which included transcriptome data from spaceflight and ground control environments. Hardware, ecotype, environmental conditions, and other experimental conditions varied, allowing the observations of overarching gene expression impacts of microgravity on plant life without focusing on effects of specific experimental conditions. Using GeneLab pre-processed datasets as the basis for the study, RNA microarray data were analyzed to identify genes that showed altered expression in microgravity when compared to control samples for each individual GLDS. All differentially expressed genes were compared to locate differentially expressed genes common between spaceflight experiments. The most noteworthy result is that not one gene shared differential expression among the six GLDS’s. However, gene expression was not influenced randomly by the microgravity environment, as there were several gene ontology terms that were significantly enriched across all experiments. These included 20 significantly enriched biological processes, and although the genes which enriched each term varied, there were many cases of specific genes common to clusters of multiple GLDS’s. Gene expression such as NAC92 and ERF011 or membrane structural element FFP6 provide insight and direction toward understanding the plant response to spaceflight. Characterizing these common processes and the shared differentially expressed genes has demonstrated potential targets for further study to understand and modulate the biological response of plants in microgravity. Life on Earth has never been subjected to the absence of gravity as a selective pressure, so observing how life forms react to a microgravity environment could provide insight to our shared fundamental biological processes. It is also feasible that the genetic modification of specific genes linked to the microgravity response could improve health and yield of space crops.

Joseph Emhof↗
NASA NTRS1995

Synchronous dynamics of a coupled shaft/bearing/housing system with auxiliary support from a clearance bearing: Analysis and experiment

This study examines the response of a flexible rotor supported by load sharing between linear bearings and an auxiliary clearance bearing. The objective is to develop a better understanding of the dynamical behavior of a magnetic bearing supported rotor system interacting with auxiliary bearings during a critical operating condition. Of particular interest is the effect of coupling between the bearing/housing and shaft vibration on the rotordynamical responses. A simulation model is developed and a number of studies are performed for various parametric configurations. An experimental investigation is also conducted to compare and verify the rotordynamic behavior predicted by the simulation studies. A strategy for reducing synchronous shaft vibration through appropriate design of coupled shaft/bearing/housing vibration modes is identified.

Lawen, James L., Jr.↗
NASA NTRS1988

The dream of a nation

A challenging new era is under consideration and on its way. With it, comes new problems and questions to solve and answer. Nevertheless, positive thinking is required of all those who participate in or share part of the Space Station's responsibility. Hence, no longer is space an unknown, unreachable environment. It is simply a place for men and women to live, work, and learn.

Martinez, Alfredo↗
NASA NTRS1993

Performance analysis and kernel size study of the Lynx real-time operating system

This paper analyzes the Lynx real-time operating system (LynxOS), which has been selected as the operating system for the Space Station Freedom Data Management System (DMS). The features of LynxOS are compared to other Unix-based operating system (OS). The tools for measuring the performance of LynxOS, which include a high-speed digital timer/counter board, a device driver program, and an application program, are analyzed. The timings for interrupt response, process creation and deletion, threads, semaphores, shared memory, and signals are measured. The memory size of the DMS Embedded Data Processor (EDP) is limited. Besides, virtual memory is not suitable for real-time applications because page swap timing may not be deterministic. Therefore, the DMS software, including LynxOS, has to fit in the main memory of an EDP. To reduce the LynxOS kernel size, the following steps are taken: analyzing the factors that influence the kernel size; identifying the modules of LynxOS that may not be needed in an EDP; adjusting the system parameters of LynxOS; reconfiguring the device drivers used in the LynxOS; and analyzing the symbol table. The reductions in kernel disk size, kernel memory size and total kernel size reduction from each step mentioned above are listed and analyzed.

Liu, Yuan-Kwei↗
NASA NTRS1994

The Buffer Diagnostic Prototype: A fault isolation application using CLIPS

This paper describes problem domain characteristics and development experiences from using CLIPS 6.0 in a proof-of-concept troubleshooting application called the Buffer Diagnostic Prototype. The problem domain is a large digital communications subsystems called the real-time network (RTN), which was designed to upgrade the launch processing system used for shuttle support at KSC. The RTN enables up to 255 computers to share 50,000 data points with millisecond response times. The RTN's extensive built-in test capability but lack of any automatic fault isolation capability presents a unique opportunity for a diagnostic expert system application. The Buffer Diagnostic Prototype addresses RTN diagnosis with a multiple strategy approach. A novel technique called 'faulty causality' employs inexact qualitative models to process test results. Experimental knowledge provides a capability to recognize symptom-fault associations. The implementation utilizes rule-based and procedural programming techniques, including a goal-directed control structure and simple text-based generic user interface that may be reusable for other rapid prototyping applications. Although limited in scope, this project demonstrates a diagnostic approach that may be adapted to troubleshoot a broad range of equipment.

Porter, Ken↗
NASA NTRS2014

Hindlimb Suspension as a Model to Study Ophthalmic Complications in Microgravity Status Report: Optimization of Rat Retina Flat Mounts Staining to Study Vascular Remodeling

Preliminary data from a prior tissue‐sharing experiment has suggested that early growth response protein‐1 (Egr1), a transcription factor involved in various stress responses in the vasculature, is induced in the rat retina after 14 days of hindlimb suspension (HS) and may be evidence that mechanical stress is occurring secondary to the cephalad fluid shift. This mechanical stress could cause changes in oxygenation of the retina, and the subsequent ischemia‐ or inflammation‐driven hypoxia may lead to microvascular remodeling. This microvascular remodeling process can be studied using image analysis of retinal vessels and can be then be quantified by the VESsel GENeration Analysis (VESGEN) software, a computational tool that quantifies remodeling patterns of branching vascular trees and capillary or vasculogenic networks. Our project investigates whether rodent HS is a valid model to study the effects of simulated‐weightlessness on ocular structures and their relationship with intracranial pressure (ICP). One of the hypotheses to be tested is that HS‐induced cephalad fluid shift is accompanied by vascular engorgement that produces changes in retinal oxygenation, leading to oxidative stress, hypoxia, microvascular remodeling, and cellular degeneration. We have optimized the procedure to obtain flat mounts of rat retina, staining of the endothelial lining in vasculature and acquisition of high quality images suitable for VESGEN analysis. Briefly, eyes were fixed in 4% paraformaldehyde for 24 hours and retinas were detached and then mounted flat on microscope slides. The microvascular staining was done with endothelial cell‐specific isolectin binding, coupled to Alexa‐488 fluorophore. Image acquisition at low magnification and high resolution was performed using a new Leica SP8 confocal microscope in a tile pattern across the X,Y plane and multiple sections along the Z‐axis. This new confocal microscope has the added capability of dye separation using the Linear Unmixing method and allows us to remove the autofluorescence originating from the photoreceptor layer. In summary, we have an improved method for studying the retinal microvasculature that will provide an increase in the quality of images captured and will be applied throughout the various animal cohorts of the recentlyinitiated study that will evaluate rodent HS as a model to study ophthalmic complications in microgravity.

Theriot, Corey A.↗
NASA NTRS2017

Exploring Connectivity in Sequence Space of Functional RNA

Emergence of replicable genetic molecules was one of the marking points in the origin of life, evolution of which can be conceptualized as a walk through the space of all possible sequences. A theoretical concept of fitness landscape helps to understand evolutionary processes through assigning a value of fitness to each genotype. Then, evolution of a phenotype is viewed as a series of consecutive, single-point mutations. Natural selection biases evolution toward peaks of high fitness and away from valleys of low fitness. whereas neutral drift occurs in the sequence space without direction as mutations are introduced at random. Large networks of neutral or near-neutral mutations on a fitness landscape, especially for sufficiently long genomes, are possible or even inevitable. Their detection in experiments, however, has been elusive. Although a few near-neutral evolutionary pathways have been found, recent experimental evidence indicates landscapes consist of largely isolated islands. The generality of these results, however, is not clear, as the genome length or the fraction of functional molecules in the genotypic space might have been insufficient for the emergence of large, neutral networks. Thorough investigation on the structure of the fitness landscape is essential to understand the mechanisms of evolution of early genomes. RNA molecules are commonly assumed to play the pivotal role in the origin of genetic systems. They are widely believed to be early, if not the earliest, genetic and catalytic molecules, with abundant biochemical activities as aptamers and ribozymes, i.e. RNA molecules capable, respectively, to bind small molecules or catalyze chemical reactions. Here, we present results of our recent studies on the structure of the sequence space of RNA ligase ribozymes selected through in vitro evolution. Several hundred thousands of sequences active to a different degree were obtained by way of deep sequencing. Analysis of these sequences revealed several large clusters defined such that every sequence in a cluster can be reached from any other sequence in the same cluster through a series of single point mutations. Sequences in a single cluster appear to adopt more than one secondary structure. The mechanism of refolding within a single cluster was examined. To shed light on possible evolutionary paths in the space of ribozymes, the connectivity between clusters was investigated. The effect of length of RNA molecules on the structure of the fitness landscape and possible evolutionary paths was examined by way of comparing functional sequences of 20 and 80 nucleobases in length. It was found that sequences of different lengths shared secondary structure motifs that were presumed responsible for catalytic activity, with increasing complexity and global structural rearrangements emerging in longer molecules.

Wei, Chenyu↗
NASA NTRS1992

Synchronous dynamics of a coupled shaft/bearing/housing system with auxiliary support from a clearance bearing: Analysis and experiment

This study examines the response of a flexible rotor supported by load sharing between linear bearings and an auxiliary clearance bearing. The objective of the work is to develop a better understanding of the dynamical behavior of a magnetic bearing supported rotor system interacting with auxiliary bearings during a critical operating condition. Of particular interest is the effect of coupling between the bearing/housing and shaft vibration on the rotordynamical responses. A simulation model is developed and a number of studies are performed for various parametric configurations. An experimental investigation is also conducted to compare and verify the rotordynamic behavior predicted by the simulation studies. A strategy for reducing synchronous shaft vibration through appropriate design of coupled shaft/bearing/housing vibration modes is identified. The results are presented and discussed.

Lawen, James, Jr.↗
NASA NTRS2012

Performance Requirements for Emergency Responder Interoperable and Compatible Electronic Safety Equipment

Firefighters and other emergency response personnel are presented with an increasing array of technologies to improve their health and safety. This includes real-time bidirectional communication, navigation and positional information, data on physiological and metabolic functions, as well as data on their surrounding environment. The emerging challenge is to integrate these elements into a practical system, addressing such features as power, data transfer, and inter-element coordination and communication. In many respects, NASA has addressed these aspects in the context of Extra Vehicular Activity (EVA). The EVA environment shares many common attributes with that of emergency response scenarios. A similar situation exists in terms of the need for interoperability among the various system sub-elements. A brief overview is presented on the similarities and differences in these two applications, as well as the technical approach adopted by NASA in terms of system design philosophy.

Greenberg, Paul S.↗