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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 91 records · Page 5
All sourcesNASA NTRSDOE OSTI
DOE OSTI2014

A microfluidic flow-cell for the study of the ultrafast dynamics of biological systems

The study of biochemical dynamics by ultrafast spectroscopic methods is often restricted by the limited amount of liquid sample available, while the high repetition rate of light sources can induce photodamage. In order to overcome these limitations, we designed a high flux, sub-ml, capillary flow-cell. While the 0.1 mm thin window of the 0.5 mm cross-section capillary ensures an optimal temporal resolution and a steady beam deviation, the cell-pump generates flows up to ~0.35 ml/s that are suitable to pump laser repetition rates up to ~14 kHz, assuming a focal spot-diameter of 100 μm. In addition, a decantation chamber efficiently removes bubbles and allows, via septum, for the addition of chemicals while preserving the closed atmosphere. The minimal useable amount of sample is ~250 μl.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2015

Note: Small anaerobic chamber for optical spectroscopy

The study of oxygen-sensitive biological samples requires an effective control of the atmosphere in which they are housed. In this aim however, no commercial anaerobic chamber is adequate to solely enclose the sample and small enough to fit in a compact spectroscopic system with which analysis can be performed. Furthermore, spectroscopic analysis requires the probe beam to pass through the whole chamber, introducing a requirement for adequate windows. In response to these challenges, we present a 1 l anaerobic chamber that is suitable for broad-band spectroscopic analysis. This chamber has the advantage of (1) providing access, via a septum, to the sample and (2) allows the sample position to be adjusted while keeping the chamber fixed and hermetic during the experiment.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2017

Laser-induced modification of structure and shape of cartilage in otolaryngology and orthopaedics

We present the results of basic research in laser modification of tissues in otolaryngology (correcting the shape of nasal septum and larynx cartilages), cosmetology (correcting ear and nose shape), orthopaedics and spinal surgery (treatment of diseases of spine disc and joints). The physical processes and mechanisms of laser-induced relaxation of stresses and regeneration of tissues are considered. New results of studies in this fast-developing field of laser surgery are presented, in particular, the results of laser correction of costal cartilage shape in the process of making implants for the treatment of larynx stenosis and controlled regeneration of the hyaline articular cartilage. (laser medicine)

62 RADIOLOGY AND NUCLEAR MEDICINE↗
DOE OSTI2021

Dynamic Transcriptomic and Phosphoproteomic Analysis During Cell Wall Stress in Aspergillus nidulans

The fungal cell-wall integrity signaling (CWIS) pathway regulates cellular response to environmental stress to enable wall repair and resumption of normal growth. This complex, interconnected, pathway has been only partially characterized in filamentous fungi. To better understand the dynamic cellular response to wall perturbation, a β-glucan synthase inhibitor (micafungin) was added to a growing A. nidulans shake-flask culture. From this flask, transcriptomic and phosphoproteomic data were acquired over 10 and 120 min, respectively. To differentiate statistically-significant dynamic behavior from noise, a multivariate adaptive regression splines (MARS) model was applied to both data sets. Over 1800 genes were dynamically expressed and over 700 phosphorylation sites had changing phosphorylation levels upon micafungin exposure. Twelve kinases had altered phosphorylation and phenotypic profiling of all non-essential kinase deletion mutants revealed putative connections between PrkA, Hk-8–4, and Stk19 and the CWIS pathway. Our collective data implicate actin regulation, endocytosis, and septum formation as critical cellular processes responding to activation of the CWIS pathway, and connections between CWIS and calcium, HOG, and SIN signaling pathways.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2015

Spectroscopy of Li Λ 9 by electroproduction

Background: In the absence of accurate data on the free two-body hyperon-nucleon interaction, the spectra of hypernuclei can provide information on the details of the effective hyperon-nucleon interaction. Purpose: To obtain a high-resolution spectrum for the 9 Be(e,e'K + ) 9 Λ Li reaction. Method: Electroproduction of the hypernucleus 9 Λ Li has been studied for the first time with sub-MeV energy resolution in Hall A at Jefferson Lab on a 9 Be target. In order to increase the counting rate and to provide unambiguous kaon identification, two superconducting septum magnets and a Ring Imaging CHerenkov detector (RICH) were added to the Hall A standard equipment. Results: The cross section to low-lying states of 9 Λ Li is concentrated within 3 MeV of the ground state and can be fitted with four peaks. The positions of the doublets agree with theory while a disagreement could exist with respect to the relative strengths of the peaks in the doublets. A Λ separation energy, B Λ , of 8.36±0.08 (stat.) ±0.08 (syst.) MeV was measured, in agreement with an earlier experiment.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2019

High-resolution hypernuclear spectroscopy at Jefferson Lab, Hall A

The experiment E94-107 in Hall A at Jefferson Lab started a systematic study of high resolution hypernuclear spectroscopy in the 0 p -shell region of nuclei such as the hypernuclei produced in electroproduction on 9 Be, 12 C and 16 O targets. In order to increase counting rates and provide unambiguous kaon identification two superconducting septum magnets and a ring-imaging Cherenkov detector were added to the Hall A standard equipment. The high-quality beam, the good spectrometers and the new experimental devices allowed us to obtain very good results. Here, measurable strength with sub-MeV energy resolution was recorded for the core-excited states of $^{12}_{Λ}$B. A high-quality$^{16}_{Λ}N hypernuclear spectrum was likewise obtained. A first measurement of the Λ binding energy for Lambda 16N, calibrated against the elementary reaction on hydrogen, was obtained with high precision, 13.76 ± 0.16 MeV. Similarly, the first$^{9}_{Λ}Li hypernuclear spectrum shows general agreement with theory (distorted-wave impulse approximation with the SLA and BS3 electroproduction models and shell-model wave functions). Some disagreement exists with respect to the relative strength of the states making up the first multiplet. A Λ separation energy of 8.36 MeV was obtained, in agreement with previous results. It has been observed that the electroproduction of hypernuclei can provide information complementary to that obtained with hadronic probes and the γ-ray spectroscopy technique.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2019

Novel T9 loop conformation of filamenting temperature-sensitive mutant Z from Mycobacterium tuberculosis

As of 2017, tuberculosis had infected 1.7 billion people (23% of the population of the world) and caused ten million deaths. Mycobacterium tuberculosis (Mtb) is quickly evolving, and new strains are classified as multidrug resistant. Thus, the identification of novel druggable targets is essential to combat the proliferation of these drug-resistant strains. Filamenting temperature-sensitive mutant Z (FtsZ) is a key protein involved in cytokinesis, an important process for Mtb proliferation and viability. FtsZ is required for bacterial cell division because it polymerizes into a structure called the Z-ring, which recruits accessory division proteins to the septum. Here, the crystal structure of the MtbFtsZ protein has been determined to 3.46 Å resolution and is described as a dimer of trimers, with an inter-subunit interface between protomers AB and DE . In this work, a novel conformation of MtbFtsZ is revealed involving the T9 loop and the nucleotide-binding pocket of protomers BC and EF .

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2018

Cell cycle‐dependent regulation of FtsZ in Escherichia coli in slow growth conditions

Summary FtsZ is the key regulator of bacterial cell division. It initiates division by forming a dynamic ring‐like structure, the Z‐ring, at the mid‐cell. What triggers the formation of the Z‐ring during the cell cycle is poorly understood. In Escherichia coli , the common view is that FtsZ concentration is constant throughout its doubling time and therefore regulation of assembly is controlled by some yet‐to‐be‐identified protein‐protein interactions. Using a newly developed functional, fluorescent FtsZ reporter, we performed a quantitative analysis of the FtsZ concentration throughout the cell cycle under slow growth conditions. In contrast to the common expectation, we show that FtsZ concentrations vary in a cell cycle‐dependent manner, and that upregulation of FtsZ synthesis correlates with the formation of the Z‐ring. The first half of the cell cycle shows an approximately fourfold upregulation of FtsZ synthesis, followed by its rapid degradation by ClpXP protease in the last 10% of the cell cycle. The initiation of rapid degradation coincides with the dissociation of FtsZ from the septum. Altogether, our data suggest that the Z‐ring formation in slow growth conditions in E. coli is partially controlled by a regulatory sequence wherein upregulation of an essential cell cycle factor is followed by its degradation.

Männik, Jaana↗
DOE OSTI2015

Characterization of cardiac quiescence from retrospective cardiac computed tomography using a correlation-based phase-to-phase deviation measure

Purpose: Accurate knowledge of cardiac quiescence is crucial to the performance of many cardiac imaging modalities, including computed tomography coronary angiography (CTCA). To accurately quantify quiescence, a method for detecting the quiescent periods of the heart from retrospective cardiac computed tomography (CT) using a correlation-based, phase-to-phase deviation measure was developed. Methods: Retrospective cardiac CT data were obtained from 20 patients (11 male, 9 female, 33–74 yr) and the left main, left anterior descending, left circumflex, right coronary artery (RCA), and interventricular septum (IVS) were segmented for each phase using a semiautomated technique. Cardiac motion of individual coronary vessels as well as the IVS was calculated using phase-to-phase deviation. As an easily identifiable feature, the IVS was analyzed to assess how well it predicts vessel quiescence. Finally, the diagnostic quality of the reconstructed volumes from the quiescent phases determined using the deviation measure from the vessels in aggregate and the IVS was compared to that from quiescent phases calculated by the CT scanner. Three board-certified radiologists, fellowship-trained in cardiothoracic imaging, graded the diagnostic quality of the reconstructions using a Likert response format: 1 = excellent, 2 = good, 3 = adequate, 4 = nondiagnostic. Results: Systolic and diastolic quiescent periods were identified for each subject from the vessel motion calculated using the phase-to-phase deviation measure. The motion of the IVS was found to be similar to the aggregate vessel (AGG) motion. The diagnostic quality of the coronary vessels for the quiescent phases calculated from the aggregate vessel (P{sub AGG}) and IVS (P{sub IV} {sub S}) deviation signal using the proposed methods was comparable to the quiescent phases calculated by the CT scanner (P{sub CT}). The one exception was the RCA, which improved for P{sub AGG} for 18 of the 20 subjects when compared to P{sub CT} (P{sub CT} = 2.48; P{sub AGG} = 2.07, p = 0.001). Conclusions: A method for quantifying the motion of specific coronary vessels using a correlation-based, phase-to-phase deviation measure was developed and tested on 20 patients receiving cardiac CT exams. The IVS was found to be a suitable predictor of vessel quiescence. The diagnostic quality of the quiescent phases detected by the proposed methods was comparable to those calculated by the CT scanner. The ability to quantify coronary vessel quiescence from the motion of the IVS can be used to develop new CTCA gating techniques and quantify the resulting potential improvement in CTCA image quality.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2023

The role of CenKR in the coordination of Rhodobacter sphaeroides cell elongation and division

ABSTRACT Cell elongation and division are essential aspects of the bacterial life cycle that must be coordinated for viability and replication. The impact of misregulation of these processes is not well understood as these systems are often not amenable to traditional genetic manipulation. Recently, we reported on the CenKR two-component system (TCS) in the Gram-negative bacterium Rhodobacter sphaeroides that is genetically tractable, widely conserved in α-proteobacteria, and directly regulates the expression of components crucial for cell elongation and division, including genes encoding subunit of the Tol-Pal complex. In this work, we show that overexpression of cenK results in cell filamentation and chaining. Using cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET), we generated high-resolution two-dimensional (2D) images and three-dimensional (3D) volumes of the cell envelope and division septum of wild-type cells and a cenK overexpression strain finding that these morphological changes stem from defects in outer membrane (OM) and peptidoglycan (PG) constriction. By monitoring the localization of Pal, PG biosynthesis, and the bacterial cytoskeletal proteins MreB and FtsZ, we developed a model for how increased CenKR activity leads to changes in cell elongation and division. This model predicts that increased CenKR activity decreases the mobility of Pal, delaying OM constriction, and ultimately disrupting the midcell positioning of MreB and FtsZ and interfering with the spatial regulation of PG synthesis and remodeling. IMPORTANCE By coordinating cell elongation and division, bacteria maintain their shape, support critical envelope functions, and orchestrate division. Regulatory and assembly systems have been implicated in these processes in some well-studied Gram-negative bacteria. However, we lack information on these processes and their conservation across the bacterial phylogeny. In R. sphaeroides and other α-proteobacteria, CenKR is an essential two-component system (TCS) that regulates the expression of genes known or predicted to function in cell envelope biosynthesis, elongation, and/or division. Here, we leverage unique features of CenKR to understand how increasing its activity impacts cell elongation/division and use antibiotics to identify how modulating the activity of this TCS leads to changes in cell morphology. Our results provide new insight into how CenKR activity controls the structure and function of the bacterial envelope, the localization of cell elongation and division machinery, and cellular processes in organisms with importance in health, host-microbe interactions, and biotechnology.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2016

A C-band broadband ortho-mode transducer for radioastronomy polarimetry

Background: We describe the design, the construction and performance of a narrow band ortho-mode transducer, currently used in the 5 GHz polarimetric receiver of the Galactic Emission Mapping project. Results: The ortho-mode transducer was designed to achieve a high degree of transmission within the 400 MHz of the GEM band around the 5 GHz (4.8–5.2 GHz). It is composed of a circular-to-square waveguide transition, a septum polarizer, a thin waveguide coupler and a smooth square-to-rectangular waveguide transition with custom waveguide bends to the output ports. Conclusion: Our simulations and measurements show a very low level of cross-polarization of about -60 dB and a good impedance match for all three ports (S11; S22; S33 < -30 dB) with only 0:25 dB of insertion loss offset across the 400 MHz (4.8–5.2 GHz) of the reception bandwidth.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2026

Water isotope data for the TEMPEST study site, 2023-2026

This dataset contains water isotope (deuterium (dD) and oxygen (d18O)) data from porewater, experimental source water, and aquifer sources from the Terrestrial Ecosystem Manipulation to Probe the Effects of Storm Treatments (TEMPEST) experimental field site in Edgewater, MD. Samples were taken from 2023-2026 and included in file "TEMPEST_Water_Isotope_LANL_2023-2026.csv". Porewater samples were taken from 15cm depth at multiple locations in each experimental plot (i.e., control, freshwater, and saltwater). Water samples were stored in 2 ml amber glass vials with septum caps under refrigeration until analysis. A L2140-i Picarro cavity ringdown spectrometer connected to an A0211 Picarro vaporization module was used to run water samples. Standards (United States Geological Survey (USGS) 47 and 48) were used to check instrument accuracy during each run of samples. Post-processing codes were run to calculate isotopic values from raw data. Reported isotope data is post-processed. Raw data CSV files from the Picarro were processed using a Python script and output as excel files containing data calibrated to Vienna Standard Mean Ocean Water (VSMOW). Standardization is based on USGS47 and USGS48 standards that are measured before and after every 5-10 unknowns. Every measurement is comprised of 10 injections. Processing involved discarding the first 4 injections due to a known memory effect. Raw means of the last 6 injections are taken for each measurement and then corrected from the linear calibration relationship of the USGS standards expected vs measured values to obtain corrected compositions relative to VSMOW. Reported isotope data are corrected mean results.

Aquifer↗
DOE OSTI2018

Optimization of Diffuser (Pre-Scatterer) Configurations for Slow Extraction Loss Reduction at Electrostatic Septa

Uncontrolled beam loss at the electrostatic septum is a performance limit for several existing or planned high power hadron accelerators delivering slow-extracted spills to fixed targets. A passive diffuser, or pre-scatterer, in a suitable configuration has been shown to reduce such beamloss significantly, with the actual gain factor depending on the parameters and details of the extraction process and hardware. In this paper, the optimization of diffuser configurations is investigated for a range of beam energies and extraction conditions, and the sensitivity to the available parameters explored via simulation results. The advantages and limitations of the diffuser are discussed and conclusions drawn concerning the specific case studies of the 8 GeV Fermilab debuncher ring and 400 GeV CERN SPS.

43 PARTICLE ACCELERATORS↗
DOE OSTI2026

Current status of the electron transport line from RCS to ESR: RTE line

The electron injection system of the U.S. Electron-Ion Collider (EIC) is located outside of the RHIC tunnel. Electrons beams accelerated by the Rapid Cycling Synchrotron (RCS) must be transported to the Electron Storage Ring (ESR), which resides within the RHIC tunnel. To accomplish this, a dedicated beam transport line, referred to as RTE (RCS-to-ESR) line is being designed. The proposed conceptual design comprises three main sections; RCS extraction, a vertical bend and dispersion suppression region, and ESR injection matching. The extraction section uses pulsed kickers and septum magnets to achieve a total deflection angle of 3 degrees. To align the injection section with ESR, the beamline must provide a vertical elevation of 1.68 m, and an array of FODO cells is used to suppress the vertical dispersion. The total length of the RTE line is approximately 133 m, and this paper presents the current design status and considerations for this transport line.

Accelerator Physics↗
DOE OSTI2013

A Simple Model to Optimize Third Integer Resonance Extraction Parameters

In storage rings with slow resonant extraction one of the main goals of optimization is the extraction inefficiency, or the fraction of beam lost due to hitting the septum plane. A simple analytical model based on a perturbation theoretic approach is built to help determine optimal parameters. It also helps to evaluate the design performance in time and in a range of machine parameters.

Nagaslaev, Vladimir↗
DOE OSTI2015

A measurement of the proton’s spin structure function g2 at low Q2

JLab E08-027, a measurement of g2p and the longitudinal-transverse (LT) spin polarizability, successfully collected data from March to May, 2012. Nucleon spin structure study has been an active research area, which has attracted a very large effort from both experimentalists and theorists. The spin structure study for the last 2 decades has provided us with many exciting and often surprising results. Recently, new precision results in the low-to-intermediate momentum transfer Q2 region from JLab have provided extensive information on the nucleon structure in the confinement region and the transition region between asymptotic free to confinement. In particular, the extensive comparisons of experimental results with Chiral Perturbation Theory (the effective theory of QCD at low energy) calculations show general good agreements, but strong disagreement in the case of the neutron LT spin polarizability. This experiment completed the measurements of gp2 and the LT spin polarizability on the proton in the low-to-intermediate Q2 region. The experiment used a polarized proton (NH3) target for the first time in Hall A. Scattered electrons were detected by a pair of Hall A high resolution spectrometer (HRS) with a pair of septum magnets. To avoid too much depolarization of the target, beam current was limited to 50-100 nA during the experiment. Since the existing beam current monitors (BCMs), beam position monitors (BPMs) and calibration methods did not work at such a low current range, new BPM and BCM receivers were designed and used for current condition. A pair of super-harps and a tungsten calorimeter were installed to calibrate the BPMs and BCMs. To compensate for the effect of the 2.5/5T transverse magnet field, two chicane dipole magnets were installed. A pair of slow rasters were installed for the first time in Hall A, combining with a pair of fast raster. The standard Hall A DAQ system and the improved high resolution DAQ system were used to record the detector information and the helicity dependent beam information, respectively. In order to achieve the required accuracy of the beam position and angle and reconstruct them event by event at the target location, the data of the BPMs and harps were carefully analyzed. The final uncertainty of BCM after the calibration using the tungsten calorimeter is below 1%, which is important for the asymmetry extraction. Before the acceptance and the dilution factor are available, the models from fitting to the world data were used instead to extract the unpolarized cross section and the dilution factor. The longitudinal and transverse physics asymmetries were extracted and compared with the model, with the study of the radiative correction. The preliminary g1 and g2 results were then extracted using the measured asymmetry and the model.

Zhu, Pengjia↗
DOE OSTI2018

Modification of the Booster Extraction Straight Section

In the process of conceptual design of the NSLS-II booster we developed an extraction layout based on a single strong pulsed septum turning the beam outside the booster vacuum chamber. This layout may not be ideal since pulsed power supply is not reproducible and its shot-to-shot voltage fluctuations will lead to an increase in the effective emittance of the extracted beam. Alternative solution1 is to divide the extraction in two magnets – a weak pulsed magnet and a strong DC magnet. This concept has been implemented in the current extraction layout.

42 ENGINEERING↗
DOE OSTI2016

Loss Control and Monitoring Systems for NSLS-Ii

The tunnel shielding design for the NSLS-II will provide adequate protection for beam losses up to 13nC/min or 100% of the maximum top-off injection beam current to be lost in the two periods of the ring around the injection point, while the remainder of the ring is shielded for lower losses of 1.1nC/min or ≤10% of the maximum top-off injected beam current. Additional local shielding of potential loss points in the injection region (e.g. injection septum) will be used to increase the level of loss allowed at these location during 1 Hz injection (i.e. nonTop-off injection). This shielding design will require a system to insure that beam losses don’t exceed these levels for a period of time that could potentially cause levels of radiation outside the shield walls to exceed administrative levels. This Loss Control and Monitoring (LCM) system will provide a level of loss control, loss location measurements and provide alarm indication of the level of beam power losses along the beam path from the source (e-gun, linac) thru the injection system and the storage ring. This system will consist of collimators and scrapers that will provide limits to the beam miss-steering and control the loss points of the charge, and monitors that will measure the average beam current losses along the beam path and alarm when this beam power loss exceeds the level set by the shielding specifications or administrative levels. This will require some new ideas in beam loss detection capability and collimation. The system is not intended to trip interlocks (dumping beam or terminating injection) but only to alarm that higher than normal beam power losses are observed and report where it is occurring. If the alarms conditions are not corrected within a time limit, top-off injection could be slowed down, thereby reducing the beam loss and the radiation it may be producing.

36 MATERIALS SCIENCE↗