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89 records · Page 5

Stimulated single fiber electromyography in the mouse: techniques and normative data

As the number of new transgenic mouse models of human neuromuscular disease continues to increase, the development of sophisticated electrophysiologic techniques for assessing the peripheral nervous system in these models has become important. Neuromuscular junction (NMJ) dysfunction, in particular, is often not detectable by morphologic or other techniques. To enable sensitive testing of murine NMJ function, we developed and tested a method for stimulated single fiber electromyography (S-SFEMG) in the gastrocnemius muscles of anesthetized mice. Jitter was assessed by measuring the mean consecutive latency difference (MCD) of single fiber responses to sciatic nerve stimulation at 2 HZ. Mean MCD values in normothermic mice were in the range of 6-8 micros for different strains, with no MCD values exceeding 25 micros. Reduced core temperature (to 29 degrees--30 degrees C) resulted in increased jitter, whereas intubation and mechanical ventilation of mice did not alter these values. Intraperitoneal and intravenous injection of vecuronium, however, resulted in progressively increased jitter followed by blocking in continuously monitored fibers. These observations validate the utility of S-SFEMG in mice as an index of NMJ function under a variety of physiologic conditions, and suggest that a high safety factor for neuromuscular transmission exists at mouse NMJs. Copyright 2001 John Wiley & Sons, Inc.

Non-NASA Center↗

Physics of Hard Spheres Experiment: Significant and Quantitative Findings Made

Direct examination of atomic interactions is difficult. One powerful approach to visualizing atomic interactions is to study near-index-matched colloidal dispersions of microscopic plastic spheres, which can be probed by visible light. Such spheres interact through hydrodynamic and Brownian forces, but they feel no direct force before an infinite repulsion at contact. Through the microgravity flight of the Physics of Hard Spheres Experiment (PHaSE), researchers have sought a more complete understanding of the entropically driven disorder-order transition in hard-sphere colloidal dispersions. The experiment was conceived by Professors Paul M. Chaikin and William B. Russel of Princeton University. Microgravity was required because, on Earth, index-matched colloidal dispersions often cannot be density matched, resulting in significant settling over the crystallization period. This settling makes them a poor model of the equilibrium atomic system, where the effect of gravity is truly negligible. For this purpose, a customized light-scattering instrument was designed, built, and flown by the NASA Glenn Research Center at Lewis Field on the space shuttle (shuttle missions STS 83 and STS 94). This instrument performed both static and dynamic light scattering, with sample oscillation for determining rheological properties. Scattered light from a 532- nm laser was recorded either by a 10-bit charge-coupled discharge (CCD) camera from a concentric screen covering angles of 0 to 60 or by sensitive avalanche photodiode detectors, which convert the photons into binary data from which two correlators compute autocorrelation functions. The sample cell was driven by a direct-current servomotor to allow sinusoidal oscillation for the measurement of rheological properties. Significant microgravity research findings include the observation of beautiful dendritic crystals, the crystallization of a "glassy phase" sample in microgravity that did not crystallize for over 1 year in 1g (Earth's gravity), and the emergence of face-centered-cubic (FCC) crystals late in the coarsening process (as small crystallites lost particles to the slow ripening of large crystallites). Significant quantitative findings from the microgravity experiments have been developed describing complex interactions among crystallites during the growth process, as concentration fields overlap in the surrounding disordered phase. Time-resolved Bragg scattering under microgravity captures one effect of these interactions quite conclusively for the sample at a volume fraction of 0.528. From the earliest time until the sample is almost fully crystalline, the size and overall crystallinity grow monotonically, but the number of crystallites per unit volume (number density) falls. Apparently nucleation is slower than the loss of crystallites because of the transfer of particles from small to large crystals. Thus, coarsening occurs simultaneously with growth, rather than following the completion of nucleation and growth as is generally assumed. In the same sample, an interesting signature appears in the apparent number density of crystallites and the volume fraction within the crystallites shortly before full crystallinity is reached. A brief upturn in both indicates the creation of more domains of the size of the average crystallite simultaneous with the compression of the crystallites. Only the emergence of dendritic arms offers a reasonable explanation. The arms would be "seen" by the light scattering as separate domains whose smaller radii of curvature would compress the interior phase. In fiscal year 1999, numerous papers, a doctoral dissertation, and the PHaSE final report were produced. Although this flight project has been completed, plans are in place for a follow-on colloid experiment by Chaikin and Russel that employs a light microscope within Glenn's Fluids and Combustion Facility on the International Space Station. PHaSE is providing us with a deeper understanding of the nure of phase transitions. The knowledge derived has added to the understanding of condensed matter. In addition, the burgeoning study of the dynamics of colloidal self-assembly may lead to the development of a range of photonic materials that control the desirable properties of light. Thus, applications of ordered colloidal structures include not only ultrastructure ceramics, but also photonic crystals and photothermal nanosecond light-switching devices. Industries dealing with semiconductors, electro-optics, ceramics, and composites stand to benefit from such advancements.

Doherty, Michael P.↗

Gamma-ray observations of Ophiuchus with EGRET: The diffuse emission and point sources

Observations of the Ophiuchus region made with the Energetic Gamma-Ray Experiment Telescope (EGRET) on the Compton Gamma Ray Observatory (CGRO) during the first 2 1/2 years of operation show the diffuse emission from the interstellar gas in Ophiuchus as well as variable emission from two point sources. The gamma-ray emission is modeled in terms of cosmic-ray interactions with atomic and molecular hydrogen in Ophiuchus and with low-energy photons along the line of sight. The model also includes the flux from the two point sources and an isotropic diffuse contribution. The cosmic-ray density is assumed to be uniform. The derived ratio of molecular hydrogen column density to integrated CO intensity is (1.1 +/- 0.2) x 10(exp 20) H-mols/sq cm (K km/s)(exp -1). At the sensitivity and resolution of the gamma-ray data, no variation of this ratio over the modeled region is discernible, nor are any regions of enhanced cosmic-ray density apparent. The model was fitted to seven narrow energy bands to obtain the energy depedence of the gamma-ray production function and the spectra of the point sources. The derived production function is in good agreement with theoretical calculations and the local cosmic-ray electron and proton spectra. The positions of the point sources were determined from maximum likelihood analysis of the gamma-ray emission observed in excess of the diffuse model. We identify one point source with the quasar PKS 1622-253, which has an average flux, E greater than 100 MeV, of (2.5 +/- 0.5) x 10(exp -7) photons/sq cm/s and photon spectral index -1.9 +/- 0.3. The other source, denoted GRO J1631-27, has not yet been identified at other wavelengths. Its average flux, E greater than 100 MeV, is (1.1 +/- 0.4) x 10(exp -7) photons/sq cm/s; however, its spectral index is poorly determined. The spectral index and intensity of the isotropic contribution to the model agree well with the extragalactic diffuse emission derived from the SAS 2 data.

Hunter, S. D.↗

Experiences performing conceptual design optimization of transport aircraft

Optimum Preliminary Design of Transports (OPDOT) is a computer program developed at NASA Langley Research Center for evaluating the impact of new technologies upon transport aircraft. For example, it provides the capability to look at configurations which have been resized to take advantage of active controls and provide and indication of economic sensitivity to its use. Although this tool returns a conceptual design configuration as its output, it does not have the accuracy, in absolute terms, to yield satisfactory point designs for immediate use by aircraft manufacturers. However, the relative accuracy of comparing OPDOT-generated configurations while varying technological assumptions has been demonstrated to be highly reliable. Hence, OPDOT is a useful tool for ascertaining the synergistic benefits of active controls, composite structures, improved engine efficiencies and other advanced technological developments. The approach used by OPDOT is a direct numerical optimization of an economic performance index. A set of independent design variables is iterated, given a set of design constants and data. The design variables include wing geometry, tail geometry, fuselage size, and engine size. This iteration continues until the optimum performance index is found which satisfies all the constraint functions. The analyst interacts with OPDOT by varying the input parameters to either the constraint functions or the design constants. Note that the optimization of aircraft geometry parameters is equivalent to finding the ideal aircraft size, but with more degrees of freedom than classical design procedures will allow.

Arbuckle, P. D.↗

The hard X-ray emission spectra from accretion columns in intermediate polars

We consider the hard (greater than 2 keV) X-ray emission from accretion columns in an intermediate polar system, GK Per, using a simple settling solution. The rate of photon emission per logarithmic energy interval can be fitted with a power law, E(exp -gamma), with gamma approximately 2.0, in agreement with observations. This index is only weakly dependent on the mass accretion rate, dot-M, for dot-M in the range of a few times 10(exp 16-18) g/s. The peak energy of the photon spectra (after photoelectric absorption) is expected to be E(sub p) approximately (5 keV) gamma(exp -1/3) (N(sub H)/10(exp 23)/sq cm)(exp 1/3) where N(sub H) is the hydrogen column density along the line of sight. The observed spectra of GK Per and possibly of V1223 Sgr suggest N(sub H) approximately 10(exp 23)/sq cm. This large N(sub H) may be due to partially ionized preshock column material. Alternatively, we also consider absorption by the cool outer parts of an accretion disk. In this case the photoelectric absorption depth in the disk is a sensitive function of inclination. For GK Per the required inclination is approximately 83 deg. For mass accretion rates larger than a critical rate of approximately 10(exp 18) g/s, X-ray emission from the column accretion is significantly affected by radiation drag. Although the mass accretion rate increases dramatically during outbursts, the observed hard (greater than 2 keV) X-ray luminosity will not rise proportionately. The slope and peak energy of the outburst spectra are only weakly affected. We conclude that the observed X-ray spectra can be explained by this simple analytic solution and that the production of hard X-rays from the accretion shock at the magnetic poles in the intermediate polars is in general agreement with the observations. However, since the X-ray emission and absorption depend on the mass accretion rate in a complicated manner, observed hard X-ray luminosities (greater than 2 keV) are not a good indicator of the mass accretion rate in the X-ray emitting regions.

Yi, Insu↗

Feasibility of Coupling Between a Single-Mode Elliptical-Core Fiber and a Single Mode Rib Waveguide Over Temperature

To determine the feasibility of coupling the output of an optical fiber to a rib waveguide in a temperature environment ranging from 20 C to 300 C, a theoretical calculation of the coupling efficiency between the two was investigated. This is a significant problem which needs to be addressed to determine whether an integrated optic device can function in a harsh temperature environment. Because the behavior of the integrated-optic device is polarization sensitive, a polarization-preserving optic fiber, via its elliptical core, was used to couple light with a known polarization into the device. To couple light energy efficiently from an optical fiber into a channel waveguide, the design of both components should provide for well-matched electric field profiles. The rib waveguide analyzed was the light input channel of an integrated-optic pressure sensor. Due to the complex geometry of the rib waveguide, there is no analytical solution to the wave equation for the guided modes. Approximation or numerical techniques must be utilized to determine the propagation constants and field patterns of the guide. In this study, three solution methods were used to determine the field profiles of both the fiber and guide: the effective-index method (EIM), Marcatili's approximation, and a Fourier method. These methods were utilized independently to calculate the electric field profile of a rib channel waveguide and elliptical fiber at two temperatures, 20 C and 300 C. These temperatures were chosen to represent a nominal and a high temperature that the device would experience. Using the electric field profile calculated from each method, the theoretical coupling efficiency between the single-mode optical fiber and rib waveguide was calculated using the overlap integral and results of the techniques compared. Initially, perfect alignment was assumed and the coupling efficiency calculated. Then, the coupling efficiency calculation was repeated for a range of transverse offsets at both temperatures. Results of the calculation indicate a high coupling efficiency can be achieved when the two components were properly aligned. The coupling efficiency was more sensitive to alignment offsets in the y direction than the x, due to the elliptical modal profile of both components. Changes in the coupling efficiency over temperature were found to be minimal.

Tuma, Margaret L.↗

Impact of the Ozone Monitoring Instrument Row Anomaly on the Long-Term Record of Aerosol Products

Since about three years after the launch the Ozone Monitoring Instrument (OMI) on the EOS-Aura satellite, the sensor's viewing capability has been affected by what is believed to be an internal obstruction that has reduced OMI's spatial coverage. It currently affects about half of the instrument's 60 viewing positions. In this work we carry out an analysis to assess the effect of the reduced spatial coverage on the monthly average values of retrieved aerosol optical depth (AOD), single scattering albedo (SSA) and the UV Aerosol Index (UVAI) using the 2005-2007 three-year period prior to the onset of the row anomaly. Regional monthly average values calculated using viewing positions 1 through 30 were compared to similarly obtained values using positions 31 through 60, with the expectation of finding close agreement between the two calculations. As expected, mean monthly values of AOD and SSA obtained with these two scattering-angle dependent subsets of OMI observations agreed over regions where carbonaceous or sulphate aerosol particles are the predominant aerosol type. However, over arid regions, where desert dust is the main aerosol type, significant differences between the two sets of calculated regional mean values of AOD were observed. As it turned out, the difference in retrieved desert dust AOD between the scattering-angle dependent observation subsets was due to the incorrect representation of desert dust scattering phase function. A sensitivity analysis using radiative transfer calculations demonstrated that the source of the observed AOD bias was the spherical shape assumption of desert dust particles. A similar analysis in terms of UVAI yielded large differences in the monthly mean values for the two sets of calculations over cloudy regions. On the contrary, in arid regions with minimum cloud presence, the resulting UVAI monthly average values for the two sets of observations were in very close agreement. The discrepancy under cloudy conditions was found to be caused by the parameterization of clouds as opaque Lambertian reflectors. When properly accounting for cloud scattering effects using Mie theory, the observed UVAI angular bias was significantly reduced. The analysis discussed here has uncovered important algorithmic deficiencies associated with the model representation of the angular dependence of scattering effects of desert dust aerosols and cloud droplets. The resulting improvements in the handling of desert dust and cloud scattering have been incorporated in an improved version of the OMAERUV algorithm.

Atmospheric Chemistry↗

Aerosol size distributions obtained by inversion of spectral optical depth measurements

Columnar aerosol size distributions have been inferred by numerically inverting particulate optical depth measurements as a function of wavelength. An inversion formula which explicitly includes the magnitude of the measurement variances is derived and applied to optical depth measurements obtained in Tucson with a solar radiometer. It is found that the individual size distributions of the aerosol particles (assumed spherical), at least for radii greater than or approximately equal to 0.1 micron, fall into one of three distinctly different categories. Approximately 50% of all distributions examined thus far can best be represented as a composite of a Junge distribution plus a distribution of relatively monodispersed larger particles centered at a radius of about 0.5 micron. Scarcely 20% of the distributions yielded Junge size distributions, while 30% yielded relatively monodispersed distributions of the log-normal or gamma distribution types. A representative selection of each of these types will be presented and discussed. The sensitivity of spectral attenuation measurements to the radii limits and refractive index assumed in the numerical inversion will also be addressed.

King, M. D.↗

Radiation, Smoke and Clouds Observed in the Southeastern Atlantic With the Research Scanning Polarimeter During ORACLES

The ObseRvations of Aerosols above Clouds and their interactions (ORACLES) project is making a series of field deployments to the southeastern Atlantic with NASA ER-2 and P3 aircraft to acquire both detailed remote sensing observations and in situ measurements of the aerosols and clouds in that region. This area is home to one of the largest low-level cloud decks on Earth that is seasonally affected by vast plumes of smoke from biomass burning, which in effect provides a natural experiment testing the radiative and microphysical interactions between the smoke and the clouds. The downward solar radiation at the surface, or cloud top, is always reduced by the presence of smoke. However, whether the amount of sunlight reflected back out to space is increased, or decreased by the presence of smoke is sensitively dependent on the brightness of the clouds and the fraction of light that the smoke absorbs each time light hits a smoke particle. In this study we use data from the Research Scanning Polarimeter, an along track scanning instrument, that provides measurements of the Stokes parameters I, Q and U at 410, 470, 555, 670, 865, 960, 1590, 1880 and 2260 nm at 150 viewing angles over a range of +/- 60° from nadir for each contiguous sub-aircraft pixel (~ 300 m in size). A retrieval algorithm is applied to the data acquired with a table look up technique, similar to that of the operational POLDER algorithm, to provide a first guess of the complex refractive index, optical depth and size distribution of the smoke particles together with cloud droplet size and optical depth. A subsequent iterative fitting procedure, where the fact that the doubling/adding method allows the construction of the Green's function for the radiative transfer equation, is used to obtain an efficient and statistically optimal estimate of the aerosol and cloud retrieval parameters. These retrieval parameters are evaluated against in situ observations, when available, and the optical depth and intensive lidar variables that are measured by the High Spectral Resolution Lidar 2. Finally, the aerosol and cloud retrievals are used to evaluate the variations in top of the atmosphere, surface/cloud top shortwave radiative forcing and atmospheric absorption that are caused by variations in the smoke and clouds.

Cairns, Brian↗

Open-source Numerical Modeling of Solidification Cracking Susceptibility: Application to Refractory Alloy Systems

Introduction. Alloys such as aluminum, nickel-base, and austenitic stainless steels are susceptible to solidification cracking during welding and 3D printing. Compositional optimization is one method used to effectively mitigate solidification cracking of those alloy systems. With the surge in hypersonic and in-space propulsion activities, refractory metals (Nb, Mo, Ta, W, and Re) and their alloy derivatives are increasing in importance due to their extreme high melting point and retention of high-temperature strength; however, their chemistry was most typically optimized to promote ductility during mechanical operations such as drawing and forming. Welding of such alloys has been a challenge due to a number of issues including solidification cracking, atmospheric contamination (O, C, and N), as well as a shift in ductile-to-brittle transition to higher temperature following grain growth induced by welding. Compositional optimization of refractory alloys for solidification cracking resistance in particular is desirable as their usage increases with the advent of advanced manufacturing methods such as 3D printing. This work evaluates the effect of compositional variation in refractory metal systems on the solidification cracking susceptibility with the goals of optimizing existing alloys and joining process techniques, and formulating new alloys with increased solidification cracking resistance. Experimental Procedures. A python code was developed in a Jupyter notebook environment (Michael and Sowards, 2023) to facilitate the calculation of crack susceptibility index proposed by Kou (2015). Composition is entered as a single point, or as a 1-D or 2-D array. The notebook calls pycalphad (Otis and Liu, 2017 and Bocklund et al, 2020) to calculate the evolution of fraction solid as a function of temperature (under either Scheil or equilibrium assumptions) and then evaluates steepness of the fraction solid curve near the terminal stage of solidification to predict solidification cracking resistance. Open source thermodynamic databases available at online repositories are used (van de Walle). The process is setup in an automated fashion to generate plots that show variation in solidification cracking susceptibility according to composition on 1-D line plots or 2-D contour plots. The Jupyter notebook and crack susceptibility algorithm was also integrated with a widely used commercial CALPHAD code for validation and alloy exploration. Results and Discussion. The crack susceptibility model was first validated against a series of refractory alloy compositions evaluated in past work which utilized a specialized Varestraint test built inside a vacuum chamber environment (Lessman and Gold, 1971). The alloys tested in the Varestraint apparatus included T-111 (Ta-8W-2Hf), ASTAR-811C (Ta-8W-1Re-0.7Hf-0.025C), FS-85 (Nb-27Ta-10W-1Zr), T-222 (Ta-9.6W-2.4Hf-0.01C), Ta-10W, B-66 (Nb-5Mo-5V-1Zr), and SCb-291 (Nb-10W-10Ta). The initial test of the model showed a strong correlation with empirical Varestraint data, i.e., a Spearman rank correlation between model predictions and hot cracking measurements was observed to be greater than 0.8. Following the validation, a set of refractory metal binary mixtures was investigated to evaluate sensitivity of Nb, Mo, W, and Ta to C, N, and O content. A series of plots were produced that suggest ppmw ranges of C, N, and O where solidification cracking increases significantly and reaches a maximum. Also comparative ranking of each primary refractory metal to each interstitial was produced. For example C produces greater cracking response in Mo whereas O produces greater cracking response in Ta and Nb. Such compositional values have utility in setting limits on pickup of these interstitial elements during welding and printing rather than using a one-size-fits-all approach. Furthermore, the results have use in determining additive powder recycling requirements, which is especially pertinent for refractory metal powders due to their high cost compared to conventional alloys. Another application created thousands of hypothetical alloys within the nominal specified composition range of two widely used refractory alloys C103 (Nb-10Hf-1Ti) and TZM (Mo-0.5Ti-0.1Zr). The cracking index was calculated for the alloys and results were fed into machine learning regression techniques including Multiple Linear Regression, Ridge Regression, and Lasso Regression to determine relative potency each alloying element had on computed solidification cracking index. A series of linear equations were produced that relate composition of C103 and TZM to solidification cracking index. The crack susceptibility of C103 for example is described by an equation of the form: cracking index ~ O + 0.667*C + 0.635*N + 0.00037*Ta – 0.0008*Hf (in wt.%) From that equation, it is clear that O has strong propensity to induce solidification cracking. Interestingly, Hf is shown to reduce calculated cracking response. Finally, realizing the potential of this method to discover new refractory alloy formulations across the period table that have low solidification cracking sensitivity, the code was applied to new untested alloy systems including W-Zr-C, W-Ta-C, and others. Conclusions. In summary, an open source numerical method has been developed using Python code to calculate Kou’s crack susceptibility index. The method was applied to refractory metals which are inherently difficult to study from a weldability testing standpoint since inert shielding gas is not sufficient and welding is typically done in vacuum, especially in light of findings presented here where oxygen has profound influence on solidification cracking. This work revealed the effect of compositional variations on a series of refractory metals and showed the framework defined here will be useful in 1) the development of new alloys that have improved weldability and 3D printability, 2) placing compositional limits on existing alloys, and 3) ensuring adequate controls of manufacturing processes such as 3D printing where powder reuse is critical. Keywords. pycalphad; Python; refractory metals; solidification cracking. References. B. Bocklund et. al. (2020) http://doi.org/10.5281/zenodo.3630657. S. Kou. (2015) https://doi.org/10.1016/j.actamat.2015.01.034. G.G. Lessmann and R.E. Gold. Welding Journal, issue 1, pp. 1-s – 8-s (1971). F.N. Michael and J.W. Sowards. NASA/TM-20230002218 (2023). R. Otis and Z.-K. Liu. (2017) http://doi.org/10.5334/jors.140. A. Van de Wallle et. al. (2018) https://doi.org/10.1016/j.calphad.2018.04.003.

pycalphad↗

Satellite Observation of El Nino Effects on Amazon Forest Phenology and Productivity

Climate variability may affect the functioning of Amazon moist tropical forests, and recent modeling analyses suggest that the carbon dynamics of the region vary interannually in response to precipitation and temperature anomalies. However, due to persistent orbital and atmospheric artifacts in the satellite record, remote sensing observations have not provided quantitative evidence that climate variation affects Amazon forest phenology or productivity, We developed a method to minimize and quantify non-biological artifacts in NOAA AVHRR satellite data, providing a record of estimated forest phenological variation from 1982-1993. The seasonal Normalized Difference Vegetation Index (NDVI) amplitude (a proxy for phenology) increased throughout much of the basin during El Nino periods when rainfall was anomalously low. Wetter La Nina episodes brought consistently smaller NDVI amplitudes. Using radiative transfer and terrestrial biogeochemical models driven by these satellite data, we estimate that canopy-energy absorption and net primary production of Amazon forests varied interannually by as much as 21% and 18%, respectively. These results provide large-scale observational evidence for interannual sensitivity to El Nino of plant phenology and carbon flux in Amazon forests.

Asner, Gregory P.↗

Scattering-Type Surface-Plasmon-Resonance Biosensors

Biosensors of a proposed type would exploit scattering of light by surface plasmon resonance (SPR). Related prior biosensors exploit absorption of light by SPR. Relative to the prior SPR biosensors, the proposed SPR biosensors would offer greater sensitivity in some cases, enough sensitivity to detect bioparticles having dimensions as small as nanometers. A surface plasmon wave can be described as a light-induced collective oscillation in electron density at the interface between a metal and a dielectric. At SPR, most incident photons are either absorbed or scattered at the metal/dielectric interface and, consequently, reflected light is greatly attenuated. The resonance wavelength and angle of incidence depend upon the permittivities of the metal and dielectric. An SPR sensor of the type most widely used heretofore includes a gold film coated with a ligand a substance that binds analyte molecules. The gold film is thin enough to support evanescent-wave coupling through its thickness. The change in the effective index of refraction at the surface, and thus the change in the SPR response, increases with the number of bound analyte molecules. The device is illuminated at a fixed wavelength, and the intensity of light reflected from the gold surface opposite the ligand-coated surface is measured as a function of the angle of incidence. From these measurements, the angle of minimum reflection intensity is determined

Wang, Yu↗

Monitoring task loading with multivariate EEG measures during complex forms of human-computer interaction

Electroencephalographic (EEG) recordings were made while 16 participants performed versions of a personal-computer-based flight simulation task of low, moderate, or high difficulty. As task difficulty increased, frontal midline theta EEG activity increased and alpha band activity decreased. A participant-specific function that combined multiple EEG features to create a single load index was derived from a sample of each participant's data and then applied to new test data from that participant. Index values were computed for every 4 s of task data. Across participants, mean task load index values increased systematically with increasing task difficulty and differed significantly between the different task versions. Actual or potential applications of this research include the use of multivariate EEG-based methods to monitor task loading during naturalistic computer-based work.

NASA Discipline Space Human Factors↗

Cutting out the Middleman: Calibrating and Validating a Dynamic Vegetation Model (ED2-PROSPECT5) Using Remotely Sensed Surface Reflectance

Canopy radiative transfer is the primary mechanism by which models relate vegetation composition and state to the surface energy balance, which is important to light- and temperature-sensitive plant processes as well as understanding land–atmosphere feedbacks. In addition, certain parameters (e.g., specific leaf area, SLA) that have an outsized influence on vegetation model behavior can be constrained by observations of shortwave reflectance, thus reducing model predictive uncertainty. Importantly, calibrating against radiative transfer outputs allows models to directly use remote sensing reflectance products without relying on highly derived products (such as MODIS leaf area index) whose assumptions may be incompatible with the target vegetation model and whose uncertainties are usually not well quantified. Here, we created the EDR model by coupling the two-stream representation of canopy radiative transfer in the Ecosystem Demography model version 2 (ED2) with a leaf radiative transfer model (PROSPECT-5) and a simple soil reflectance model to predict full-range, high-spectral-resolution surface reflectance that is dependent on the underlying ED2 model state. We then calibrated this model against estimates of hemispherical reflectance (corrected for directional effects) from the NASA Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) and survey data from 54 temperate forest plots in the northeastern United States. The calibration significantly reduced uncertainty in model parameters related to leaf biochemistry and morphology and canopy structure for five plant functional types. Using a single common set of parameters across all sites, the calibrated model was able to accurately reproduce surface reflectance for sites with highly varied forest composition and structure. However, the calibrated model's predictions of leaf area index (LAI) were less robust, capturing only 46 % of the variability in the observations. Comparing the ED2 radiative transfer model with another two-stream soil–leaf–canopy radiative transfer model commonly used in remote sensing studies (PRO4SAIL) illustrated structural errors in the ED2 representation of direct radiation backscatter that resulted in systematic underestimation of reflectance. In addition, we also highlight that, to directly compare with a two-stream radiative transfer model like EDR, we had to perform an additional processing step to convert the directional reflectance estimates of AVIRIS to hemispherical reflectance (also known as “albedo”). In future work, we recommend that vegetation models add the capability to predict directional reflectance, to allow them to more directly assimilate a wide range of airborne and satellite reflectance products. We ultimately conclude that despite these challenges, using dynamic vegetation models to predict surface reflectance is a promising avenue for model calibration and validation using remote sensing data.

Alexey N Shiklomanov↗

Galvanic Vestibular Reduction Modifies Perception of Coriolis Cross-Coupling and Delays Motion Sickness Onset

INTRODUCTION: Alterations in vestibular sensory processing following G-transitions lead to head movement sensitivity and motion sickness upon return to Earth’s gravity. The purpose of this study was to evaluate whether a non-pharmaceutical tool using galvanic vestibular reduction (GVR) could suppress disorienting illusions and mitigate motion sickness. A similar approach using anodal (inhibitory) currents delivered to both ears has been shown to result in a selective reversible ablation of irregular vestibular afferents [1]. METHODS: Using a repeated measures counter-balanced design, motion sickness and perception were obtained in 26 subjects during Coriolis cross-coupling stimuli on a rotating chair across three GVR treatment interventions: throughout stimulus testing (prevention), following symptom onset (rescue), and placebo control. The GVR peak current was maintained at 2.5 mA across subjects and across prevention / rescue sessions. Subjects performed up to 10 sets of pitch head movements during constant rotation. For each set, head movement was cued every 10 seconds, alternating between pitch forward (chin resting to chest) and pitch backward (head upright) for a total of 7 forward and backward movements. During each head movement, subjects were asked to use a joystick to record the magnitude of their perceived rotation along three axes. During the 2-minute pause between sets, motion sickness symptom scoring was obtained using the Pensacola Diagnostic Index and subject discomfort (0-20) ratings. Performance on a sensorimotor and cognitive test battery was measured during a fourth session to map changes in GVR level with functional performance. RESULTS: Fourteen of the 26 subjects were not susceptible to the motion stressor (i.e., did not reach an endpoint in the control condition). While the time to endpoint, or number of head movements, did not significantly vary across the three GVR conditions in the remaining subjects, the symptom levels were significantly lower through the third set of head movements when GVR was on throughout the testing. Initiating GVR following symptom onset did not appear to alter the symptom progression nor time to motion sickness endpoint. Based on the joystick measures, GVR significantly modified the perceived roll and pitch sensation during head movements, reducing the amplitude of tilt in most subjects. It is important to note that comparable levels of GVR did not impair performance on a functional test battery including mobility, balance and cognitive tasks. DISCUSSION: Our findings suggest GVR may be useful in reducing disorienting roll and pitch illusions and delaying the onset of motion sickness. Further enhancements will be required to individualize the stimulation amplitude and optimize the waveform delivery. Adapting this non-pharmaceutical countermeasure approach to allow self-administered titration of current amplitude during recovery would enable transfer to post-flight treatment of motion sickness. [1] Minor L. B. and Goldberg J. M. (1991) J Neurosci 11, 1636-48. 109, 889-894.

G N Pradhan↗

Diurnal and Seasonal Variations in Chlorophyll Fluorescence Associated with Photosynthesis at Leaf and Canopy Scales

There is a critical need for sensitive remote sensing approaches to monitor the parameters governing photosynthesis, at the temporal scales relevant to their natural dynamics. The photochemical reflectance index (PRI) and chlorophyll fluorescence (F) offer a strong potential for monitoring photosynthesis at local, regional, and global scales, however the relationships between photosynthesis and solar induced F (SIF) on diurnal and seasonal scales are not fully understood. This study examines how the fine spatial and temporal scale SIF observations relate to leaf level chlorophyll fluorescence metrics (i.e., PSII yield, YII and electron transport rate, ETR), canopy gross primary productivity (GPP), and PRI. The results contribute to enhancing the understanding of how SIF can be used to monitor canopy photosynthesis. This effort captured the seasonal and diurnal variation in GPP, reflectance, F, and SIF in the O2A (SIFA) and O2B (SIFB) atmospheric bands for corn (Zea mays L.) at a study site in Greenbelt, MD. Positive linear relationships of SIF to canopy GPP and to leaf ETR were documented, corroborating published reports. Our findings demonstrate that canopy SIF metrics are able to capture the dynamics in photosynthesis at both leaf and canopy levels, and show that the relationship between GPP and SIF metrics differs depending on the light conditions (i.e., above or below saturation level for photosynthesis). The sum of SIFA and SIFB (SIFA+B), as well as the SIFA+B yield, captured the dynamics in GPP and light use efficiency, suggesting the importance of including SIFB in monitoring photosynthetic function. Further efforts are required to determine if these findings will scale successfully to airborne and satellite levels, and to document the effects of data uncertainties on the scaling.

Campbell, Petya K.↗

Using Whispering-Gallery-Mode Resonators for Refractometry

A method of determining the refractive and absorptive properties of optically transparent materials involves a combination of theoretical and experimental analysis of electromagnetic responses of whispering-gallery-mode (WGM) resonator disks made of those materials. The method was conceived especially for use in studying transparent photorefractive materials, for which purpose this method affords unprecedented levels of sensitivity and accuracy. The method is expected to be particularly useful for measuring temporally varying refractive and absorptive properties of photorefractive materials at infrared wavelengths. Still more particularly, the method is expected to be useful for measuring drifts in these properties that are so slow that, heretofore, the properties were assumed to be constant. The basic idea of the method is to attempt to infer values of the photorefractive properties of a material by seeking to match (1) theoretical predictions of the spectral responses (or selected features thereof) of a WGM of known dimensions made of the material with (2) the actual spectral responses (or selected features thereof). Spectral features that are useful for this purpose include resonance frequencies, free spectral ranges (differences between resonance frequencies of adjacently numbered modes), and resonance quality factors (Q values). The method has been demonstrated in several experiments, one of which was performed on a WGM resonator made from a disk of LiNbO3 doped with 5 percent of MgO. The free spectral range of the resonator was approximately equal to 3.42 GHz at wavelengths in the vicinity of 780 nm, the smallest full width at half maximum of a mode was approximately equal to 50 MHz, and the thickness of the resonator in the area of mode localization was 30 microns. In the experiment, laser power of 9 mW was coupled into the resonator with an efficiency of 75 percent, and the laser was scanned over a frequency band 9 GHz wide at a nominal wavelength of approximately equal to 780 nm. Resonance frequencies were measured as functions of time during several hours of exposure to the laser light. The results of these measurements, plotted in the figure, show a pronounced collective frequency drift of the resonator modes. The size of the drift has been estimated to correspond to a change of 8.5 x 10(exp -5) in the effective ordinary index of refraction of the resonator material.

Matsko, Andrey↗