Cultivation of Tomato Tissues Capable of Forming Flowers and Fruits in Vitro
The final phase of this research project was designed to develop a practical method for producing a steady supply of fresh cherry tomato fruits over a period of several months, for possible use as a fresh vegetable supplement to a standard diet of astronauts on extended missions. This effort was successful. We were able to excise immature flowers from Pixie tomato plants grown in a controlled condition room, implant them on artificial media under aseptic conditions, and get them to develop into edible fruits in a little over a month. The medium (Murashige-Skoog) was purchased from Sigma, supplemented with sugar plus a synthetic analog of the plant hormone cytokinin, and adjusted to pH 5.8. A temperature of at least 25 C and visible light helped to produce ripe red fruits within 7 weeks. To ensure a steady supply of such tomatoes, we found it possible to store the explanted flower buds in MS medium at 5 C for at least 6 weeks without significant loss of ability to develop into fruits. This means that many containers could be prepared before launch and put into a refrigerator; a convenient number could then be removed periodically to guarantee a succession of harvests during the life of an extended mission. Details are found in the attached reprints. Subsequent applications for funds for flight or continued research were denied, and the project was terminated.