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Metabolome and RNA-seq Analysis of Responses to Nitrogen Deprivation and Resupply in Tea Plant (Camellia sinensis) Roots

Nitrogen (N) is an important contributor in regulating plant growth and development as well as secondary metabolites synthesis, so as to promote the formation of tea quality and flavor. Theanine, polyphenols, and caffeine are important secondary metabolites in tea plant. In this study, the responses of Camellia sinensis roots to N deprivation and resupply were investigated by metabolome and RNA-seq analysis. N deficiency induced content increase for most amino acids (AAs) and reduction for the remaining AAs, polyphenols, and caffeine. After N recovery, the decreased AAs and polyphenols showed a varying degree of recovery in content, but caffeine did not. Meanwhile, theanine increased in content, but its related synthetic genes were down-regulated, probably due to coordination of the whole N starvation regulatory network. Flavonoids-related pathways were relatively active following N stress according to KEGG enrichment analysis. Gene co-expression analysis revealed TCS2 , AMT1;1 , TAT2 , TS , and GOGAT as key genes, and TFs like MYB, bHLH, and NAC were also actively involved in N stress responses in C. sinensis roots. These findings facilitate the understanding of the molecular mechanism of N regulation in tea roots and provide genetic reference for improving N use efficiency in tea plant.

Xu, Wenluan↗

Inter-Oligomer Interaction Influence on Photoluminescence in Cis-Polyacetylene Semiconductor Materials

Semiconducting conjugated polymers (CPs) are pivotal in advancing organic electronics, offering tunable properties for solar cells and field-effect transistors. Here, we carry out first-principle calculations to study individual cis-polyacetylene (cis-PA) oligomers and their ensembles. The ground electronic structures are obtained using density functional theory (DFT), and excited state dynamics are explored by computing nonadiabatic couplings (NACs) between electronic and nuclear degrees of freedom. We compute the nonradiative relaxation of charge carriers and photoluminescence (PL) using the Redfield theory. Our findings show that electrons relax faster than holes. The ensemble of oligomers shows faster relaxation compared to the single oligomer. The calculated PL spectra show features from both interband and intraband transitions. The ensemble shows broader line widths, redshift of transition energies, and lower intensities compared to the single oligomer. This comparative study suggests that the dispersion forces and orbital hybridizations between chains are the leading contributors to the variation in PL. It provides insights into the fundamental behaviors of CPs and the molecular-level understanding for the design of more efficient optoelectronic devices.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Lightweight helmet-mounted eye movement measurement system

The helmet-mounted eye movement measuring system, weighs 1,530 grams; the weight of the present aviators' helmet in standard form with the visor is 1,545 grams. The optical head is standard NAC Eye-Mark. This optical head was mounted on a magnesium yoke which in turn was attached to a slide cam mounted on the flight helmet. The slide cam allows one to adjust the eye-to-optics system distance quite easily and to secure it so that the system will remain in calibration. The design of the yoke and slide cam is such that the subject can, in an emergency, move the optical head forward and upward to the stowed and locked position atop the helmet. This feature was necessary for flight safety. The television camera that is used in the system is a solid state General Electric TN-2000 with a charged induced device imager used as the vidicon.

Barnes, J. A.↗

Ion microprobe zircon geochronology of the Uivak Gneisses: Implications for the evolution of early terrestrial crust in the North Atlantic Craton

Ion microprobe U-Pb results for zircons from three Uivak I gneisses and one specimen of Uivak II gneiss, from the Saglek-Hebron area of Northern Labrador are reported. These results are compared with interpretations based on published conventional U-Pb zircon results and with conclusions about crustal evolution in the NAC derived from Rb-Sr, Sm-Nd and Pb-Pb isotopic studies.

Collerson, K. D.↗

AE and DE mass spectrometer observations relevant to the shuttle glow

Recent work suggested that NO2 may be responsible for the observed continuum glow near surfaces of the space shuttle. The observations of atomic nitrogen (N) are reported at shuttle altitudes using mass spectrometers, giving special attention to the surface reactions of N relevant to the production of NO2 on spacecraft surfaces. Data from two semi-open sources mass spectrometers, the OSS instruments on the Atmosphere Explorer-C and -D satellites, and the closed source Neutral Atmospheric Composition Spectrometer (NACS) on the Dynamic Explorer-2 satellite are presented to show the similar behavior of NO in each case and the contrasting behavior of NO2. Although signals of NO and NO2 are highly dependent on surface temperature and surface composition, it appears that direct exposure of ion source surfaces to rammed gas is a necessary condition for the production of large amounts of NO2. Evidence that elevated surface temperatures can significantly reduce the production of NO2, likely by causing more rapid desorption of NO from these surfaces, is presented.

Engebretson, M. J.↗

Analysis of atomic thermospheric nitrogen density

A NASA grant provided support for a research project at Augsbury College, Minneapolis, Minnesota for the analysis of atomic nitrogen density data obtained by the Neutral Atmospheric Composition Spectrometer (NACS) on board the Dynamics Explorer-2 satellite. Initial funding was for an exploratory study of the feasibility of obtaining ambient densities of N from source densities of NO. Funding was continued under the Dynamics Explorer Guest Investigator Program when initial studies indicated probable success in obtaining such ambient densities. The major scientific focus of the later work was to be to characterize the behavior of N densities at high latitudes.

Engebretson, M. J.↗

UT/longitudinal variations of composition in DE data

Composition data obtained by the quadrupole mass spectrometer (NACS) carried aboard the DE-B satellite were examined for variations in the polar regions during magnetically quiet conditions. The corresponding predictions of the MSIS-83 model are often used for comparison. The MSIS-83 model is used to emphasize the variations of interest by suppressing all other variations. A persistent enhancement of N2 density and a depletion of He density are present in the vicinity of the magnetic poles with maximum density response in the morning hours (magnetic) on average. A universal time (UT) variation in average density levels is evident near both the geographic and magnetic poles. There are systematic morphology changes with UT and between summer and winter which are qualitatively consistent with the simple concept that thermospheric heating effects are shifted or spread in the downwind direction of the global circulation systems driven by EUV and magnetospheric sources. The magnitude of the UT variations is larger in the southern hemisphere and is larger in local winter than summer.

Hedlin, A. E.↗

The band systems of alkali vapors

A number of band edges of the molecules, Na2, K2, NaK, NaCs, LiK, LiRb, LiCs, and NaRb are arranged in edge schemes. The vibrational quanta of the base terms and the upper terms can be approximately determined. Viewpoints are produced for interpreting electron terms. The terms Na2 are interpreted as terms of a photo-electron.

Weizel, W.↗

Large Enhancements in the O/N2 Ratio in the Evening Sector of the Winter Hemisphere During Geomagnetic Storms

In this paper, we have looked for enhancements of the O/N2 ratio in data measured by the Dynamics Explorer 2 (DE 2) satellite in the middle latitudes of the winter hemisphere, based on a prediction that was made by the National Center for Atmospheric Research thermosphere/tonosphere general circulation model (NCAR-TIGCM) that such increases occur. The NCAR-TIGCM predicts that these enhancements should be seen throughout the low latitude region and in many middle latitude locations, but that the enhancements in O/N2 are particularly strong in the middle-latitude, evening-to-midnight sector of the winter hemisphere. When this prediction was used to look for these effects in DE 2 NACS (neutral atmosphere composition spectrometer) data, large enhancements in the O/N2 ratio (approx. 50 to 90%) were seen. These enhancements were observed during the main phase of a storm that occurred on November 24, 1982, and were seen in the same region of the winter hemisphere predicted by the NCAR-TIGCM. They are partially the result of the depletion of N2 and, as electron loss is dependent on dissociative recombination at F(sub 2) altitudes, they have implications for electron densities in this area. Parcel trajectories, which have been followed through the NCAR-TIGCM history file for this event, show that large O/N2 enhancements occur in this limited region in the winter hemisphere for two reasons. First, these parcels of air are decelerated by the antisunward edge of the ion convection pattern; individual parcels converge and subsidence occurs. Thus molecular-nitrogen-poor air is brought from higher to lower heights. Because neutral parcels that are found a little poleward of the equatorial edge of the eveningside convection pattern are swept inward toward the center of the auroral oval, the enhancements occur only in a very limited range of latitudes. Second, nitrogen-poor air is transported from regions close to the magnetic pole in the winter hemisphere. During geomagnetic storms, enhanced meridional winds are driven by the increased pressure-gradient force that is associated with intensified Joule heating in the auroral oval. These pressure-driven winds decrease rapidly on the dayside beyond the auroral oval where the parcels originate, limiting the region into which the parcels can be transported. Thus these two processes drive values of O/N2 in a limited region of the winter hemisphere, and reinforce only in the evening sector, causing large changes in this region.

Burns, A. G.↗

First Imaging Results from the Iapetus B/C Flyby of the Cassini Spacecraft

The first of two relatively close Iapetus flybys in Cassini's primary mission occured on Dec 31, 2004 18:49 UTC near apoapsis from orbit "B" to "C" at an altitude of approximately 123,400 km over the northern leading hemisphere, resulting in a minimum pixel scale of 740 m for the ISS narrow angle camera (NAC). Data revealed details of a greater than 1300-km-long ridge that had been discovered just one week earlier in optical navigation images. Individual mountains within the western part of the ridge reach heights of approximately 20 km over surrounding terrain. The data set provides constraints on the origin of the albedo dichotomy. It appears very likely that the dark material is overlying an ice crust, but no evidence for emplacement of dark material via surface flows is apparent. Instead, signs for dark-material emplacement through processes that included ballistic transportation are visible. No bright-floor ("punch-through") craters have been found on the dark hemisphere. The ridge discovery may revive the idea of an endogenic origin of the dark side.

Denk, T.↗

Thermal Design of the Cassini Narrow Angle Camera

The Narrow Angle Camera (NAC) is one of two cameras in the Imaging Science Subsystem (ISS) on the Cassini Spacecraft (S/C), the second camera is a Voyager-inherited Wide Angle Camera (WAC). Cassini is currently planned to be launched in October 1997 and will arrive at Saturn for a four year tour in June 2004. The Narrow Angle Optics are a Ritchey Chretien type Optics, has a focal length of 2000 mm, a relative aperture of f/10.5, a spectral range of 200 to 1100 nm, 24 filters, a pixel field of view of 6.0 microradian/pixel, and has a field of view of 3.5 x 3.5 degrees. The sensor is a Charged Couple Device (CCD), 1024 x 1024 pixels with a pixel size of 12 x 12 um, a full well greater than 50,000 e-, on chip processing of up to 800,000 e- pixel summation, a dark current of less than 0.1 e-/pixel/sec at operating temperature, and a charge transfer efficiency of 0.99999 at operating temperature...

Hoffman, Pamela↗

Cassini Camera Contamination Anomaly: Experiences and Lessons Learned

We discuss the contamination 'Haze' anomaly for the Cassini Narrow Angle Camera (NAC), one of two optical telescopes that comprise the Imaging Science Subsystem (ISS). Cassini is a Saturn Orbiter with a 4-year nominal mission. The incident occurred in 2001, five months after Jupiter encounter during the Cruise phase and ironically at the resumption of planned maintenance decontamination cycles. The degraded optical performance was first identified by the Instrument Operations Team with the first ISS Saturn imaging six weeks later. A distinct haze of varying size from image to image marred the images of Saturn. A photometric star calibration of the Pleiades, 4 days after the incident, showed stars with halos. Analysis showed that while the halo's intensity was only 1 - 2% of the intensity of the central peak of a star, the halo contained 30 - 70% of its integrated flux. This condition would impact science return. In a review of our experiences, we examine the contamination control plan, discuss the analysis of the limited data available and describe the one-year campaign to remove the haze from the camera. After several long conservative heating activities and interim analysis of their results, the contamination problem as measured by the camera's point spread function was essentially back to preanomaly size and at a point where there would be more risk to continue. We stress the importance of the flexibility of operations and instrument design, the need to do early infight instrument calibration and continual monitoring of instrument performance.

Cassini↗

A General Closed-Form Solution for the Lunar Reconnaissance Orbiter (LRO) Antenna Pointing System

The National Aeronautics and Space Administration s (NASA) Lunar Reconnaissance Orbiter (LRO) launched on June 18, 2009 from the Cape Canaveral Air Force Station aboard an Atlas V launch vehicle into a direct insertion trajectory to the Moon LRO, designed, built, and operated by the NASA Goddard Space Flight Center in Greenbelt, MD, is gathering crucial data on the lunar environment that will help astronauts prepare for long-duration lunar expeditions. During the mission s nominal life of one year its six instruments and one technology demonstrator will find safe landing site, locate potential resources, characterize the radiation environment and test new technology. To date, LRO has been operating well within the bounds of its requirements and has been collecting excellent science data images taken from the LRO Camera Narrow Angle Camera (LROC NAC) of the Apollo landing sites have appeared on cable news networks. A significant amount of information on LRO s science instruments is provided at the LRO mission webpage. LRO s Attitude Control System (ACS), in addition to controlling the orientation of the spacecraft is also responsible for pointing the High Gain Antenna (HGA). A dual-axis (or double-gimbaled) antenna, deployed on a meter-long boom, is required to point at a selected Earth ground station. Due to signal loss over the distance from the Moon to Earth, pointing precision for the antenna system is very tight. Since the HGA has to be deployed in spaceflight, its exact geometry relative to the spacecraft body is uncertain. In addition, thermal distortions and mechanical errors/tolerances must be characterized and removed to realize the greatest gain from the antenna system. These reasons necessitate the need for an in-flight calibration. Once in orbit around the moon, a series of attitude maneuvers was conducted to provide data needed to determine optimal parameters to load onboard, which would account for the environmental and mechanical errors at any antenna orientation. The nominal geometry for the HGA involves an outer gimbal axis that is exactly perpendicular to the inner gimbal axis, and a target direction that is exactly perpendicular to the outer gimbal axis. For this nominal geometry, closed-form solutions of the desired gimbal angles are simple to get for a desired target direction specified in the spacecraft body fame. If the gimbal axes and the antenna boresight are slightly misaligned, the nominal closed-form solution is not sufficiently accurate for computing the gimbal angles needed to point at a target. In this situation, either a general closed-form solution has to be developed for a mechanism with general geometries, or a correction scheme has to be applied to the nominal closed-form solutions. The latter has been adopted for Solar Dynamics Observatory (SDO) as can be seen in Reference 1, and the former has been used for LRO. The advantage of the general closed-form solution is the use of a small number of parameters for the correction of nominal solutions, especially in the regions near singularities. Singularities here refer to cases when the nominal closed-form solutions have two or more solutions. Algorithm complexity, however, is the disadvantage of the general closed-form solution.

Shah, Neerav↗

Inferred Lunar Boulder Distributions at Decimeter Scales

Block size distributions of impact deposits on the Moon are diagnostic of the impact process and environmental effects, such as target lithology and weathering. Block size distributions are also important factors in trafficability, habitability, and possibly the identification of indigenous resources. Lunar block sizes have been investigated for many years for many purposes [e.g., 1-3]. An unresolved issue is the extent to which lunar block size distributions can be extrapolated to scales smaller than limits of resolution of direct measurement. This would seem to be a straightforward statistical application, but it is complicated by two issues. First, the cumulative size frequency distribution of observable boulders rolls over due to resolution limitations at the small end. Second, statistical regression provides the best fit only around the centroid of the data [4]. Confidence and prediction limits splay away from the best fit at the endpoints resulting in inferences in the boulder density at the CPR scale that can differ by many orders of magnitude [4]. These issues were originally investigated by Cintala and McBride [2] using Surveyor data. The objective of this study was to determine whether the measured block size distributions from Lunar Reconnaissance Orbiter Camera - Narrow Angle Camera (LROC-NAC) images (m-scale resolution) can be used to infer the block size distribution at length scales comparable to Mini-RF Circular Polarization Ratio (CPR) scales, nominally taken as 10 cm. This would set the stage for assessing correlations of inferred block size distributions with CPR returns [6].

Baloga, S. M.↗

A Notional Example of Understanding Human Exploration Traverses on the Lunar Surface

Mr. Gruener received an M.S. in physical science, with an emphasis in planetary geology, from the University of Houston-Clear Lake in 1994. He then began working with NASA JSC.s Solar System Exploration Division on the development of prototype planetary science instruments, the development of a mineral-based substrate for nutrient delivery to plant growth systems in bio-regenerative life support systems, and in support of the Mars Exploration Rover missions in rock and mineral identification. In 2004, Mr. Gruener again participated in a renewed effort to plan and design missions to the Moon, Mars, and beyond. He participated in many exploration planning activities, including NASA.s Exploration Systems Architecture Study (ESAS), Global Exploration Strategy Workshop, Lunar Architecture Team 1 and 2, Constellation Lunar Architecture Team, the Global Point of Departure Lunar Exploration Team, and the NASA Advisory Council (NAC) Workshop on Science Associated with the Lunar Exploration Architecture. Mr. Gruener has also been an active member of the science team supporting NASA.s Desert Research and Technology Studies (RATS).

Gruener, John↗

MSL Lessons Learned and Knowledge Capture

The Mars Program has recently been informed of the Planetary Protection Subcommittee (PPS) recommendation, which was endorsed by the NAC, concerning Mars Science Lab (MSL) lessons learned and knowledge capture. The Mars Program has not had an opportunity to consider any decisions specific to the PPS recommendation. Some of the activities recommended by the PPS would involve members of the MSL flight team who are focused on cruise, entry descent & landing, and early surface operations; those activities would have to wait. Members of the MSL planetary protection team at JPL are still available to support MSL lessons learned and knowledge capture; some of the specifically recommended activities have already begun. The Mars Program shares the PPS/NAC concerns about loss of potential information & expertise in planetary protection practice.

planetary protection↗

Technology Needs for the Next Generation of NASA Science Missions

In-Space propulsion technologies relevant to Mars presentation is for the 14.03 Emerging Technologies for Mars Exploration panel. The talk will address propulsion technology needs for future Mars science missions, and will address electric propulsion, Earth entry vehicles, light weight propellant tanks, and the Mars ascent vehicle. The second panel presentation is Technology Needs for the Next Generation of NASA Science Missions. This talk is for 14.02 Technology Needs for the Next Generation of NASA Science Missions panel. The talk will summarize the technology needs identified in the NAC's Planetary Science Decadal Survey, and will set the stage for the talks for the 4 other panelist.

Anderson, David J.↗

Co-registration of Laser Altimeter Tracks with Digital Terrain Models and Applications in Planetary Science

We have derived algorithms and techniques to precisely co-register laser altimeter profiles with gridded Digital Terrain Models (DTMs), typically derived from stereo images. The algorithm consists of an initial grid search followed by a least-squares matching and yields the translation parameters at sub-pixel level needed to align the DTM and the laser profiles in 3D space. This software tool was primarily developed and tested for co-registration of laser profiles from the Lunar Orbiter Laser Altimeter (LOLA) with DTMs derived from the Lunar Reconnaissance Orbiter (LRO) Narrow Angle Camera (NAC) stereo images. Data sets can be co-registered with positional accuracy between 0.13 m and several meters depending on the pixel resolution and amount of laser shots, where rough surfaces typically result in more accurate co-registrations. Residual heights of the data sets are as small as 0.18 m. The software can be used to identify instrument misalignment, orbit errors, pointing jitter, or problems associated with reference frames being used. Also, assessments of DTM effective resolutions can be obtained. From the correct position between the two data sets, comparisons of surface morphology and roughness can be made at laser footprint- or DTM pixel-level. The precise co-registration allows us to carry out joint analysis of the data sets and ultimately to derive merged high-quality data products. Examples of matching other planetary data sets, like LOLA with LRO Wide Angle Camera (WAC) DTMs or Mars Orbiter Laser Altimeter (MOLA) with stereo models from the High Resolution Stereo Camera (HRSC) as well as Mercury Laser Altimeter (MLA) with Mercury Dual Imaging System (MDIS) are shown to demonstrate the broad science applications of the software tool.

Laser↗