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74 records · Page 5

Lunar Dust Effects on Spacesuit Systems: Insights from the Apollo Spacesuits

Systems and components of selected Apollo A7L/A7LB flight-article spacesuits that were worn on the lunar surface have been studied to determine the degree to which they suffered contamination, abrasion and wear or loss of function due to effects from lunar soil particles. Filter materials from the lithium hydroxide (LiOH) canisters from the Apollo Command Module were also studied to determine the amount and type of any lunar dust particles they may have captured from the spacecraft atmosphere. The specific spacesuit study materials include the outermost soft fabric layers on Apollo 12 and 17 integrated thermal micrometeorite garment assemblies and outermost fabrics on Apollo 17 extravehicular pressure gloves. In addition, the degree of surface wear in the sealed wrist rotation bearing from Apollo 16 extravehicular and intravehicular pressure gloves was evaluated and compared. Scanning electron microscope examination of the Apollo 12 T-164 woven TeflonO fabric confirms the presence of lunar soil particles and the ability of these particles to cause separation and fraying of the Teflon fibers. Optical imaging, chemical analysis and particle sampling applied to the outer fabric of the Apollo 17 spacesuit has identified Ti as a potentially useful chemical marker for comparing the amount of lunar soil retained on different areas of the spacesuit outer fabric. High-yield particle sampling from the Apollo 17 fabric surfaces using adhesive tape found 80% of particles on the fabric are lunar soil particles averaging 10.5 m in diameter, with the rest being intrinsic fabric materials or environmental contaminants. Analysis of the mineralogical composition of the lunar particles found that on a grain-count basis the particle population is dominated by plagioclase feldspar and various types of glassy particles derived mostly from soil agglutinates, with a subordinate amount of pyroxene. On a grain size basis, however, the pyroxene grains are generally a factor of 2 larger than glass and plagioclase, so conversion of the data to a modal (volume %) basis results in pyroxene becoming the modally dominant particle type with glass and plagioclase significantly less abundant. When comparisons are made to the modal composition of lunar soil at the Apollo 17 landing site, the results suggest that pyroxene particles have overall better retention on the spacesuit outer fabric compared to plagioclase and especially glass. Scanning electron microscopy revealed no measureable difference in the amount of wear and abrasion in the wrist rotation bearing of an Apollo 16 pressure glove worn only in the spacecraft and one worn only for extravehicular activity on the lunar surface. The results suggest either that the bearing prevented entry of lunar dust, or that dust was not sufficiently abrasive to damage the bearing, or both.

Christoffersen, Roy↗

Space shuttle flight (STS-45) of L8 myoblast cells results in the isolation of a nonfusing cell line variant

Myoblast cell cultures have been widely employed in conventional (1g) studies of biological processes because characteristics of intact muscle can be readily observed in these cultured cells. We decided to investigate the effects of spaceflight on muscle by utilizing a well characterized myoblast cell line (L8 rat myoblasts) as cultured in the recently designed Space Tissue Loss Flight Module "A" (STL-A). The STL-A is a "state of the art," compact, fully contained, automated cell culture apparatus which replaces a single mid-deck locker on the Space Shuttle. The L8 cells were successfully flown in the STL-A on the Space Shuttle STS-45 mission. Upon return to earth, reculturing of these spaceflown L8 cells (L8SF) resulted in their unexpected failure to fuse and differentiate into myotubes. This inability of the L8SF cells to fuse was found to be a permanent phenotypic alteration. Scanning electron microscopic examination of L8SF cells growing at 1g on fibronectin-coated polypropylene fibers exhibited a strikingly different morphology as compared to control cells. In addition to their failure to fuse into myotubes, L8SF cells also piled up on top of each other. When assayed in fusion-promoting soft agar, L8SF cells gave rise to substantially more and larger colonies than did either preflight (L8AT) or ground control (L8GC) cells. All data to this point indicate that flying L8 rat myoblasts on the Space Shuttle for a duration of 7-10 d at subconfluent densities results in several permanent phenotypic alterations in these cells.

STS-45 Shuttle Project↗