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NASA NTRS2006

A Year in the Life of International Space Station

The past twelve months (October 2005 to September 2006) have been among the busiest in the life of the International Space Station (ISS), both in terms of on-orbit operations as well as future planning, for both ISS systems and research. The Expedition 12 and 13 crews completed their missions successfully, carrying out research for Russia, the United States, Europe and Japan, and bringing continuous ISS occupancy to nearly six years. The European Space Agency's (ESA) first Long Duration Mission on ISS is underway, involving significant international research. The Expedition 14 crew completed its training and is embarking on its own 6-month mission with a full slate of international research. Future crews are in training for their respective assembly and research missions. Shuttle flights resumed after a 10-month hiatus, delivering new research facilities and resuming assembly of ISS. ESA's Columbus research module was delivered to the Kennedy Space Center, joining Japan's Kibo research module already there. Following preflight testing, the two modules will launch in 2007 and 2008, respectively, joining Destiny as ISS's research infrastructure. A revised ISS configuration and assembly sequence were endorsed by all the Partners, with a reduced number of Shuttle flights, but for the first time including plans for post-Shuttle ISS operations after 2010. The new plan will pose significant challenges to the ISS research community. As Europe and Japan build their on-orbit research infrastructure, and long-term plans become firmer, the next 12 months should prove to be equally challenging and exciting.

Uri, John J.↗

NASA NTRS2019

Characterization of Sensory and Shelter Enrichment in the Rodent Research Habitat

The ISS provides a platform for conducting Rodent Research (RR) in microgravity and 9 missions have been successfully conducted. The results from these experiments have begun to provide new insights into the effects of spaceflight on mammalian physiological systems. After RR-1-4, the Flight IACUC required inclusion of additional cage enrichment into the Rodent Habitats (RH) to "enhance animal well-being by providing animals with sensory and motor stimulation, through structures and resources that facilitate the expression of species typical behaviors". A Hut, in the form of a rigid, mesh igloo-like shelter was implemented beginning with RR-5. The potential influence of the Hut in the novel cage environment of RH on various spaceflight-sensitive physiological systems has not been fully explored. To understand the effects of the Hut, mice (female C57Bl/6J, 15wks) were housed in Vivarium cage (n=5), RH with Hut (n=5), No Hut (n=5), Nestlet (n=10), and Cocoon (n=10) for 7 weeks. There were no differences in weekly body mass or food consumption. Tail blood draw indicated no differences in plasma corticosterone levels, immune cell types, or IgA levels. 24hrs prior to euthanasia, Open Field (OF) and Novel Object (NO) tests were performed. There were no differences across groups, all mice engaged in thigmotaxis (arena wall proximity) in the OF over 50% of the recorded time, and thigmotaxis declined when a NO was introduced. Additional behavioral analysis from daily videos are in progress to quantify activity levels. Post-euthanasia, there were no differences in soleus muscle or adrenal gland mass. Analysis of distal femur cancellous revealed some differences in microarchitecture. These results show that introduction of the Hut may diminish differences observed between spaceflight and ground controls, warranting improved validation of Hut effects in space, and also underscore the value of thorough preflight, ground based testing.

Shirazi-Fard, Yasaman↗

NASA NTRS2016

On-orbit RNA Purification and qRT-PCR Capabilities of the WetLab-2 System

NASA Ames Research Center’s WetLab-2 system brings new capabilities to the ISS for researchers. The system can lyse cells and extract RNA on-orbit from different sample types ranging from microbial cultures to animal tissues. Our purification method has the advantage of using non-toxic chemicals and does not require alcohols or other organics. The purified RNA can then either be stabilized for return to Earth or can be used to conduct on-orbit quantitative Reverse Transcriptase PCR (qRT-PCR) analysis without the need for sample return. qRT-PCR reactions are performed by dispensing the RNA into reaction tubes that contain all lyophilized reagents needed to perform the analysis. The system uses a Cepheid® SmartCycler that allows for multiplexing of assays, this can be used to normalize for RNA concentration and integrity and to study multiple genes of interest in each tube. There are a total of 16 independent PCR modules each capable of detecting up to four fluorescent channels. The WetLab- 2 system can downlink data from the ISS to the ground after a completed run and uplink new thermal cycling programs. The ability to purify and stabilize RNA on-orbit can eliminate the confounding effects of reentry stresses and shock acting on live cells and organisms or the concern of RNA degradation of some samples. It also has the benefit of minimizing the needed downmass. Conducting qRT-PCR and generating results on-orbit is also an important step towards utilizing the ISS as a National Laboratory facility. Specifically, the ability to get on-orbit data will provide investigators with the opportunity to adjust experimental parameters in real time without the need for sample return and re-flight. On orbit gene expression analysis can also provide benchmarking prior to sample return. The system can also be used for analysis of air, surface, water, and clinical samples to monitor environmental pathogens and crew health. The validation flight of the WetLab-2 system using E. coli bacteria and mouse liver is scheduled to launch on SpaceX-8 this spring. Pending operations, the preliminary results from the validation flight will be presented. To support the needs of future researchers, we are adapting our system to purify RNA from two additional sample types: fibrous tissue such as muscle and mammalian adherent cells grown on alginate beads. Progress of this work will also be presented. The WetLab-2 Project is supported by the Research Integration Office in the ISS Program.

Parra, Macarena↗

NASA NTRS2012

International Space Station Research Benefits for Humanity

The ISS partnership has seen a substantial increase in research accomplished, crew efforts devoted to research, and results of ongoing research and technology development. The ISS laboratory is providing a unique environment for research and international collaboration that benefits humankind. Benefits come from the engineering development, the international partnership, and from the research results. Benefits can be of three different types: scientific discovery, applications to life on Earth, and applications to future exploration. Working across all ISS partners, we identified key themes where the activities on the ISS improve the lives of people on Earth -- not only within the partner nations, but also in other nations of the world. Three major themes of benefits to life on earth emerged from our review: benefits to human health, education, and Earth observation and disaster response. Other themes are growing as use of the ISS continues. Benefits to human health range from advancements in surgical technology, improved telemedicine, and new treatments for disease. Earth observations from the ISS provide a wide range of observations that include: marine vessel tracking, disaster monitoring and climate change. The ISS participates in a number of educational activities aimed to inspire students of all ages to learn about science, technology, engineering and mathematics. To date over 63 countries have directly participated in some aspect of ISS research or education. In summarizing these benefits and accomplishments, ISS partners are also identifying ways to further extend the benefits to people in developing countries for the benefits of humankind.

Thumm, Tracy↗

NASA NTRS2014

Spaceflight Microbiology: Benefits for Long Duration Spaceflight and Our Understanding of Microorganisms on Earth

Spaceflight microbiology is composed of both operational and experimental components that complement each other in our understanding of microbial interactions and their responses in the microgravity of spaceflight. Operationally, efforts to mitigate microbiological risk to the crew and the spacecraft have historically focused on minimizing the number of detectable organisms, relying heavily on preventative measures, including appropriate vehicle design, crew quarantine prior to flight, and stringent microbial monitoring. Preflight monitoring targets have included the astronauts, spaceflight foods, potable water systems, the vehicle air and surfaces, and the cargo carried aboard the spacecraft. This approach has been very successful for earlier missions; however, the construction and long-term habitation of the International Space Station (ISS) has created the need for additional inflight monitoring of the environment and potable water systems using hardware designed for both in-flight microbial enumeration and sample collection and return to Earth. In addition to operational activities, the ISS is providing a research platform to advance our understanding of microbiomes in the built environment. Adding to the research possibilities of this system are multiple reports of unique changes in microbial gene expression and phenotypic responses, including virulence and biofilm formation, in response to spaceflight culture. The tremendous potential of the ISS research platform led the National Research Council to recommend that NASA utilize the ISS as a microbial observatory. Collectively, the findings from operational and research activities on the ISS are expected to both enable future space exploration and translate to basic and applied research on Earth.

Ott, C. Mark↗

NASA NTRS2013

Development Status of the WetLab-2 Project: New Tools for On-orbit Real-time Quantitative Gene Expression.

The primary objective of NASA Ames Research Centers WetLab-2 Project is to place on the ISS a research platform to facilitate gene expression analysis via quantitative real-time PCR (qRT-PCR) of biological specimens grown or cultured on orbit. The WetLab-2 equipment will be capable of processing multiple sample types ranging from microbial cultures to animal tissues dissected on-orbit. In addition to the logistical benefits of in-situ sample processing and analysis, conducting qRT-PCR on-orbit eliminates the confounding effects on gene expression of reentry stresses and shock acting on live cells and organisms. The system can also validate terrestrial analyses of samples returned from ISS by providing quantitative on-orbit gene expression benchmarking prior to sample return. The ability to get on orbit data will provide investigators with the opportunity to adjust experimental parameters for subsequent trials based on the real-time data analysis without need for sample return and re-flight. Finally, WetLab-2 can be used for analysis of air, surface, water, and clinical samples to monitor environmental contaminants and crew health. The verification flight of the instrument is scheduled to launch on SpaceX-5 in Aug. 2014.Progress to date: The WetLab-2 project completed a thorough study of commercially available qRT-PCR systems and performed a downselect based on both scientific and engineering requirements. The selected instrument, the Cepheid SmartCycler, has advantages including modular design (16 independent PCR modules), low power consumption, and rapid ramp times. The SmartCycler has multiplex capabilities, assaying up to four genes of interest in each of the 16 modules. The WetLab-2 team is currently working with Cepheid to modify the unit for housing within an EXPRESS rack locker on the ISS. This will enable the downlink of data to the ground and provide uplink capabilities for programming, commanding, monitoring, and instrument maintenance. The project is currently designing a module that will lyse the cells and extract RNA of sufficient quality for use in qRT-PCR reactions while using a housekeeping gene to normalize RNA concentration and integrity. Current testing focuses on two promising commercial products and chemistries that allow for RNA extraction with minimal complexity and crew time.

quantitative PCR↗

NASA NTRS1999

JSC, NASA Lead Center: Overview of Human Space Life Sciences Programs Office (HSLSPO)

An overview of the Human Space Life Sciences Programs Office (HSLSPO) presents the following topics in viewgraph form: Agency structure, objectives of the HSLSPO lead center implementation plan, HSLSPO relationship to Johnson Space Center (JSC) as lead center, HSLSPO programs and projects, biomedical research and countermeasures, HSLSPO relationship to the International Space Station (ISS), and BR&C ISS flight research content.

Stegemoeller, Charles↗

NASA NTRS2002

Space Ops 2002: Bringing Space Operations into the 21st Century. Track 3: Operations, Mission Planning and Control. 2nd Generation Reusable Launch Vehicle-Concepts for Flight Operations

With the successful implementation of the International Space Station (ISS), the National Aeronautics and Space Administration (NASA) enters a new era of opportunity for scientific research. The ISS provides a working laboratory in space, with tremendous capabilities for scientific research. Utilization of these capabilities requires a launch system capable of routinely transporting crew and logistics to/from the ISS, as well as supporting ISS assembly and maintenance tasks. The Space Shuttle serves as NASA's launch system for performing these functions. The Space Shuttle also serves as NASA's launch system for supporting other science and servicing missions that require a human presence in space. The Space Shuttle provides proof that reusable launch vehicles are technically and physically implementable. However, a couple of problems faced by NASA are the prohibitive cost of operating and maintaining the Space Shuttle and its relative inability to support high launch rates. The 2nd Generation Reusable Launch Vehicle (2nd Gen RLV) is NASA's solution to this problem. The 2nd Gen RLV will provide a robust launch system with increased safety, improved reliability and performance, and less cost. The improved performance and reduced costs of the 2nd Gen RLV will free up resources currently spent on launch services. These resource savings can then be applied to scientific research, which in turn can be supported by the higher launch rate capability of the 2nd Gen RLV. The result is a win - win situation for science and NASA. While meeting NASA's needs, the 2nd Gen RLV also provides the United States aerospace industry with a commercially viable launch capability. One of the keys to achieving the goals of the 2nd Gen RLV is to develop and implement new technologies and processes in the area of flight operations. NASA's experience in operating the Space Shuttle and the ISS has brought to light several areas where automation can be used to augment or eliminate functions performed by crew and ground controllers. This experience has also identified the need for new approaches to staffing and training for both crew and ground controllers. This paper provides a brief overview of the mission capabilities provided by the 2nd Gen RLV, a description of NASA's approach to developing the 2nd Gen RLV, a discussion of operations concepts, and a list of challenges to implementing those concepts.

Hagopian, Jeff↗

NASA NTRS2009

International Space Station Science Research Accomplishments During the Assembly Years: An Analysis of Results from 2000-2008

This report summarizes research accomplishments on the International Space Station (ISS) through the first 15 Expeditions. When research programs for early Expeditions were established, five administrative organizations were executing research on ISS: bioastronautics research, fundamental space biology, physical science, space product development, and space flight. The Vision for Space Exploration led to changes in NASA's administrative structures, so we have grouped experiments topically by scientific themes human research for exploration, physical and biological sciences, technology development, observing the Earth, and educating and inspiring the next generation even when these do not correspond to the administrative structure at the time at which they were completed. The research organizations at the time at which the experiments flew are preserved in the appendix of this document. These investigations on the ISS have laid the groundwork for research planning for Expeditions to come. Humans performing scientific investigations on ISS serve as a model for the goals of future Exploration missions. The success of a wide variety of investigations is an important hallmark of early research on ISS. Of the investigations summarized here, some are completed with results released, some are completed with preliminary results, and some remain ongoing.

Evans, Cynthia A.↗

NASA NTRS2018

Public Engagement of Earth Science and Art Using Astronaut Photography

For over fifty years of crewed spaceflight missions, NASA astronauts have taken remarkable photographs of the Earth that offer various perspectives for viewing dynamic Earth processes. The International Space Station (ISS) today provides a unique platform to view and capture imagery of Earth at a variety of viewing angles, seasons, and times of day. Astronaut photos comprise a variable true-color (RGB - Red Green Blue) dataset taken with multiple handheld digital (and historically, film) cameras and lens types that differ from nadir-looking satellite-based remote sensing instruments with fixed temporal and spatial resolutions. The Crew Earth Observations (CEO) Facility within the Earth Science and Remote Sensing Unit at NASA’s Johnson Space Center supports the acquisition, analysis, and curation of astronaut photography of Earth’s surface and atmosphere. CEO’s website, the Gateway to Astronaut Photography of Earth (eol.jsc.nasa.gov), provides free public access to view, search, and download over three million images taken by astronauts from Mercury, Gemini, and Apollo missions to current ISS missions. Handheld images taken by astronauts from the ISS are used for scientific research and public engagement in STEM (Science, Technology, Engineering, Mathematics) fields, particularly earth science. While segments of the public are actively interested in STEM fields, there may be groups who are underexposed to and disinterested in earth science or are unaware of astronaut photography from the ISS. A broader public audience can be introduced to earth science, STEM fields, and ISS research through artistic or abstract photos of the Earth. Wide angle pictures taken of Earth have identifiable landforms and bodies of water, however zoomed in, very high resolution photos without obvious geographic reference can attract new viewing audiences on a purely aesthetic basis. The CEO Facility actively curates a collection of exceptional astronaut photos of Earth as art used to reach a wider population through increased digital products and outreach events. This continuously-updated collection feeds development of freely accessible and high-quality downloadable materials including scientific articles, videos, computer wallpapers, and social media content. These materials are presented with science-based information that augments the artistic qualities of the imagery, and facilitate connection between art-engaged or non-STEM audiences to the ISS research platform and the need for future innovative STEM research.

Meado, Andrea L.↗

NASA NTRS2011

The International Space Station: A National Science Laboratory

After more than a decade of assembly missions and on the heels of the final voyage of Space Shuttle Discovery, the International Space Station (ISS) has reached assembly completion. With visiting spacecraft now docking with the ISS on a regular basis, the Station now serves as a National Laboratory to scientists back on Earth. ISS strengthens relationships among NASA, other Federal entities, higher educational institutions, and the private sector in the pursuit of national priorities for the advancement of science, technology, engineering, and mathematics. In this lecture we will explore the various areas of research onboard ISS to promote this advancement: (1) Human Research, (2) Biology & Biotechnology, (3) Physical & Material Sciences, (4) Technology, and (5) Earth & Space Science. The ISS National Laboratory will also open new paths for the exploration and economic development of space.

Giblin, Timothy W.↗

NASA NTRS2009

Humans on the International Space Station-How Research, Operations, and International Collaboration are Leading to New Understanding of Human Physiology and Performance in Microgravity

As the International Space Station (ISS) nears completion, and full international utilization is achieved, we are at a scientific crossroads. ISS is the premier location for research aimed at understanding the effects of microgravity on the human body. For applications to future human exploration, it is key for validation, quantification, and mitigation of a wide variety of spaceflight risks to health and human performance. Understanding and mitigating these risks is the focus of NASA s Human Research Program. However, NASA s approach to defining human research objectives is only one of many approaches within the ISS international partnership (including Roscosmos, the European Space Agency, the Canadian Space Agency, and the Japan Aerospace Exploration Agency). Each of these agencies selects and implements their own ISS research, with independent but related objectives for human and life sciences research. Because the science itself is also international and collaborative, investigations that are led by one ISS partner also often include cooperative scientists from around the world. The operation of the ISS generates significant additional data that is not directly linked to specific investigations. Such data comes from medical monitoring of crew members, life support and radiation monitoring, and from the systems that have been implemented to protect the health of the crew (such as exercise hardware). We provide examples of these international synergies in human research on ISS and highlight key early accomplishments that derive from these broad interfaces. Taken as a whole, the combination of diverse research objectives, operational data, international sharing of research resources on ISS, and scientific collaboration provide a robust research approach and capability that no one partner could achieve alone.

Ronbinson, Julie A.↗

NASA NTRS2016

Expanding NASA and Roscosmos Scientific Collaboration on the International Space Station

The International Space Station (ISS) is a world-class laboratory orbiting in space. NASA and Roscosmos have developed a strong relationship through the ISS Program Partnership, working together and with the other ISS Partners for more than twenty years. Since 2013, based on a framework agreement between the Program Managers, NASA and Roscosmos are building a joint program of collaborative research on ISS. This international collaboration is developed and implemented in phases. Initially, members of the ISS Program Science Forum from NASA and TsNIIMash (representing Roscosmos) identified the first set of NASA experiments that could be implemented in the "near term". The experiments represented the research categories of Technology Demonstration, Microbiology, and Education. Through these experiments, the teams from the "program" and "operations" communities learned to work together to identify collaboration opportunities, establish agreements, and jointly plan and execute the experiments. The first joint scientific activity on ISS occurred in January 2014, and implementation of these joint experiments continues through present ISS operations. NASA and TsNIIMash have proceeded to develop "medium term" collaborations, where scientists join together to improve already-proposed experiments. A major success is the joint One-Year Mission on ISS, with astronaut Scott Kelly and cosmonaut Mikhail Kornienko, who returned from ISS in March, 2016. The teams from the NASA Human Research Program and the RAS Institute for Biomedical Problems built on their considerable experience to design joint experiments, learn to work with each other's protocols and processes, and share medical and research data. New collaborations are being developed between American and Russian scientists in complex fluids, robotics, rodent research and space biology, and additional human research. Collaborations are also being developed in Earth Remote Sensing, where scientists will share data from imaging systems mounted on ISS as well as other orbiting spacecraft to improve our understanding of the Earth and its climate. NASA and Roscosmos continue to encourage international scientific cooperation and expanded use of the ISS Laboratory. "Long-term", larger collaborations will achieve scientific objectives that no single national science team or agency can achieve on its own. The joint accomplishments achieved so far have paved the way for a stronger international scientific community and improved results and benefits from ISS.

Hasbrook, Pete↗

NASA NTRS2002

Overview of GRC's Advanced Sensor and Instrumentation Development

Glenn Research Center develops advanced diagnostic techniques to measure surface and flow properties in research facilities. We support a variety of aerospace propulsion applications: Shuttle, X-33, X-43, ISS, and research engine components: inlets, compressors, combustors, nozzles. We are developing a suite of instrumentation specifically for 3rd Generation Reusable Launch Vehicle testing.

Mercer, Carolyn↗

NASA NTRS2016

Light Microscopy Module: International Space Station Premier Automated Microscope

The Light Microscopy Module (LMM) was launched to the International Space Station (ISS) in 2009 and began hardware operations in 2010. It continues to support Physical and Biological scientific research on ISS. During 2016, if all goes as planned, three experiments will be completed: [1] Advanced Colloids Experiments with Heated base-2 (ACE-H2) and [2] Advanced Colloids Experiments with Temperature control (ACE-T1). Preliminary results, along with an overview of present and future LMM capabilities will be presented; this includes details on the planned data imaging processing and storage system, along with the confocal upgrade to the core microscope. [1] a consortium of universities from the State of Kentucky working through the Experimental Program to Stimulate Competitive Research (EPSCoR): Stuart Williams, Gerold Willing, Hemali Rathnayake, et al. and [2] from Chungnam National University, Daejeon, S. Korea: Chang-Soo Lee, et al.

Microgravity Biology Research↗

NASA NTRS2016

Using the Light Microscopy Module (LMM) on the International Space Station (ISS), The Advanced Colloids Experiment (ACE) and MacroMolecular Biophysics (MMB)

The Light Microscopy Module (LMM) was launched to the International Space Station (ISS) in 2009 and began science operations in 2010. It continues to support Physical and Biological scientific research on ISS. During 2016, if all goes as planned, three experiments will be completed: [1] Advanced Colloids Experiments with Heated base-2 (ACE-H2) and [2] Advanced Colloids Experiments with Temperature control (ACE-T1). Preliminary results, along with an overview of present and future LMM capabilities will be presented; this includes details on the planned data imaging processing and storage system, along with the confocal upgrade to the core microscope. [1] a consortium of universities from the State of Kentucky working through the Experimental Program to Stimulate Competitive Research (EPSCoR): Stuart Williams, Gerold Willing, Hemali Rathnayake, et al. and [2] from Chungnam National University, Daejeon, S. Korea: Chang-Soo Lee, et al.

Microgravity Biology Research↗

NASA NTRS2015

Light Microsopy Module, International Space Station Premier Automated Microscope

The Light Microscopy Module (LMM) was launched to the International Space Station (ISS) in 2009 and began science operations in 2010. It continues to support Physical and Biological scientific research on ISS. During 2015, if all goes as planned, five experiments will be completed: [1] Advanced Colloids Experiments with a manual sample base -3 (ACE-M-3), [2] the Advanced Colloids Experiment with a Heated Base -1 (ACE-H-1), [3] (ACE-H-2), [4] the Advanced Plant Experiment -03 (APEX-03), and [5] the Microchannel Diffusion Experiment (MDE). Preliminary results, along with an overview of present and future LMM capabilities will be presented; this includes details on the planned data imaging processing and storage system, along with the confocal upgrade to the core microscope. [1] New York University: Paul Chaikin, Andrew Hollingsworth, and Stefano Sacanna, [2] University of Pennsylvania: Arjun Yodh and Matthew Gratale, [3] a consortium of universities from the State of Kentucky working through the Experimental Program to Stimulate Competitive Research (EPSCoR): Stuart Williams, Gerold Willing, Hemali Rathnayake, et al., [4] from the University of Florida and CASIS: Anna-Lisa Paul and Rob Ferl, and [5] from the Methodist Hospital Research Institute from CASIS: Alessandro Grattoni and Giancarlo Canavese.

Microscopy↗

NASA NTRS2016

Modifying a Commercial Centrifuge to Reduce Electromagnetic Interference and Evaluating Functionality of Ultrasound Equipment

The Project Management and Engineering Branch (SF4) supports the Human Health and Performance Directorate (HH&P) and is responsible for developing and supporting human systems hardware for the International Space Station (ISS). When a principal investigator's (PI) medical research project on the ISS is accepted, SF4 develops the necessary hardware and software to transport to the ISS. The two projects I primarily worked on were the centrifuge and ultrasound projects. Centrifuge: One concern with spacecraft such as the ISS is electromagnetic interference (EMI) from onboard equipment, typically from radio waves (frequencies of ~3 kHz to ~300 GHz), which can negatively affect nearby circuitry. Standard commercial centrifuges produce EMI above safety limits, so my task was to help reduce EMI production from this equipment. Two centrifuges were tested: one unmodified as a control and one modified. To reduce EMI below safety limits, one centrifuge was modified to become a Faraday shield, in which significant electrical contact was made between all regions of the centrifuge housing. This included removing non-conductive paint, applying conductive fabric to the lid and foam sealer, adding a 10,000 μF decoupling capacitor across the power supply, and adding copper adhesive-mount gaskets to the housing interior. EMI testing of both centrifuges was performed in the EMI/EMC Control Test and Measurement Facility. EMI for both centrifuges was below safety limits for frequencies between 10 MHz and 15 GHz (pass); however, between 14 kHz and 10 MHz, EMI for the unmodified centrifuge exceeded safety limits (fail) as expected. Alternatively, for the modified centrifuge with the Faraday shield, EMI was below the safely limit of 55 dBμV/m for electromagnetic frequencies between 14 kHz and 10 MHz. This result indicates our modifications were successful. The successful EMI test allowed us to communicate with the vendor what modifications they needed to make to their commercial unit to meet our specifications and to understand what needs to be done in lab to the new centrifuge. Our modifications will provide a standard for readying centrifuges for future missions. Once the new modified centrifuge arrives by the vendor, it will need to undergo EMI testing again for validation. The centrifuge is also in the process of compatibility testing with a custom stowage drawer, which is an ongoing project in SF4. Both of these items will be payloads on future missions to the ISS for various research purposes. Ultrasound: ISS currently has an onboard ultrasound (Ultrasound 2 system) for research and medical purposes. Every piece of medical flight hardware has an equivalent ground-unit so instrumentation can be routinely evaluated and transported to the ISS if necessary. The ground-unit ultrasound equipment must be evaluated every six months using a task performance sheet (TPS). A TPS is a document, written by the appropriate scientists and engineers, which describes how to run equipment and is written in such a way that astronauts with unspecialized training can follow the tasks. I was responsible for performing six TPSs on a combination of three ultrasounds and two video power converters (VPCs). Performing a TPS involves checking out and computationally documenting each piece of equipment removed from storage locations, setting up hardware and software, performing tasks to verify functionality, returning equipment, and logging items back into the computerized system. My work revealed all ground-unit ultrasounds were functioning properly. Because of proper function, a discrepancy report (DR) did not have to be opened. The TPS was then passed along to the Quality Engineering (QE) for review and ultimately given to Quality Assurance (QA). Other projects: In addition to my main projects, I participated in other tasks including troubleshooting an EEG headband, volunteering for an ultrasound training research study, and conformal coating printed circuit boards. My internship at SF4 has helped me understand how space systems hardware development for the ISS fits into NASA's mission and vision.

Greening, Gage J.↗