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Post-Remediation Radiological Dose Assessment, Painesville Site, Painesville, Ohio

A post-remediation radiological dose assessment was conducted for the Formerly Utilized Sites Remedial Action Program (FUSRAP) Painesville Site by using the measured residual concentrations of the radionuclides of concern following the completion of remedial action. The site’s FUSRAP-related constituents of concern (COCs) are radionuclides associated with past activities conducted by the Manhattan Engineer District (MED) and Atomic Energy Commission (AEC) in support of the Nation’s early atomic energy and weapons program, and include radium-226 (Ra-226), thorium-230 (Th-230), thorium-232 (Th-232), and total uranium (Total U). Remedial actions were conducted at the site in accordance with the Final Record of Decision for the Painesville Site, Painesville, Ohio (ROD) (USACE 2006). In the ROD, the U.S. Army Corps of Engineers (USACE) determined that the U.S. Nuclear Regulatory Commission (NRC) standards for decommissioning of licensed facilities found in Title 10, Part 20.1402 of the Code of Federal Regulations (10 CFR 20.1402) and in the Ohio Administrative Code (OAC) 3701:1-38-22(B) are applicable or relevant and appropriate requirements (ARARs) for the site. On the basis of the ARAR analysis, a total effective dose equivalent (TEDE) goal of 25 millirem per year (mrem/yr) was assumed for the site with a construction worker considered as the average member of the critical group. Derived concentration guideline levels (DCGLs) for residual radioactivity of the COCs in soil were developed for the Painesville Site using the RESRAD (Version 6.22) computer code (Yu et al. 2001), which was designed to estimate radiation doses and risks from RESidual RADioactive materials. The DCGLs represent activity levels above site average background activity corresponding to the 25-mrem/yr criterion for a construction worker scenario. The DCGLs are above the background activity concentrations and represent average concentration guidelines. The DCGLs and average background activity concentrations are provided in Table 1. The Final Feasibility Study Addendum (USACE 2005) provides a complete description of the derivation of the DCGLs for the Painesville Site. A post-remediation radiological dose assessment was performed to determine the potential radiation doses to a construction worker, consistent with the approach used to develop the DCGLs for the Painesville Site given in the Final Feasibility Study Addendum (USACE 2005) and ROD (USACE 2006). A second more conservative dose assessment was performed to determine the potential radiation doses to an urban resident who is assumed to live and garden on the site. To further evaluate the long-term protectiveness of the remedial action, a third dose assessment was conducted to determine the potential radiation doses to a hypothetical subsistence farmer. The primary difference between exposure to an urban resident versus a conservative subsistence farmer would be an increase in the amount of foodstuffs (homegrown produce, milk, and beef) produced/cultivated and consumed on-site.

54 ENVIRONMENTAL SCIENCES↗

Nevada National Security Site Environmental Report 2014

This Nevada Nuclear Security Site Environmental Report (NNSSER) was prepared to satisfy DOE Order DOE O 231.1B, “Environment, Safety and Health Reporting.” Its purpose is to (1) report compliance status with environmental standards and requirements, (2) present results of environmental monitoring of radiological and nonradiological effluents, (3) report estimated radiological doses to the public from releases of radioactive material, (4) summarize environmental incidents of noncompliance and actions taken in response to them, (5) describe the NNSA/NFO Environmental Management System and characterize its performance, and (6) highlight significant environmental programs and efforts.This NNSSER summarizes data and compliance status for calendar year 2014 at the Nevada National Security Site (NNSS) (formerly the Nevada Test Site) and its two support facilities, the North Las Vegas Facility (NLVF) and the Remote Sensing Laboratory–Nellis (RSL-Nellis). It also addresses environmental restoration (ER) projects conducted at the Tonopah Test Range (TTR) and the Nevada Test and Training Range (NTTR). Through a Memorandum of Agreement, NNSA/NFO is responsible for the oversight of these ER projects, and the Sandia Field Office of NNSA (NNSA/SFO) has oversight of all other TTR and NTTR activities. NNSA/SFO produces the TTR annual environmental report, which is available at http://www.sandia.gov/news/publications/environmental/index.html.Major Site Programs and Facilities NNSA/NFO directs the management and operation of the NNSS and six sites across the nation. The six sites include two in Nevada (NLVF and RSL-Nellis) and four in other states (RSL-Andrews in Maryland, Livermore Operations in California, Los Alamos Operations in New Mexico, and Special Technologies Laboratory in California). Los Alamos, Lawrence Livermore, and Sandia National Laboratories are the principal organizations that sponsor and implement the nuclear weapons programs at the NNSS. National Security Technologies, LLC (NSTec), is the current Management and Operating contractor accountable for the successful execution of work and ensuring that work is performed in compliance with environmental regulations. The six sites all provide support to enhance the NNSS as a location for weapons experimentation and nuclear test readiness. The three major NNSS missions include National Security/Defense, Environmental Management, and Nondefense. The major programs that support these missions are Stockpile Stewardship and Management, Nonproliferation and Counterterrorism, Nuclear Emergency Response, Strategic Partnership Projects, Environmental Restoration, Waste Management, Conservation and Renewable Energy, Other Research and Development, and Infrastructure. The major facilities that support the programs include the U1a Facility, Big Explosives Experimental Facility (BEEF), Device Assembly Facility, Dense Plasma Focus Facility, Joint Actinide Shock Physics Experimental Research Facility, Radiological/Nuclear Countermeasures Test and Evaluation Complex, Nonproliferation Test and Evaluation Complex (NPTEC), Radiological/Nuclear Weapons of Mass Destruction Incident Exercise Site (known as the T-1 Site), Area 5 Radioactive Waste Management Complex (RWMC), and the Area 3 Radioactive Waste Management Site (RWMS).

54 ENVIRONMENTAL SCIENCES↗

Nevada National Security Site Environmental Report 2016

This Nevada National Security Site Environmental Report (NNSSER) was prepared to satisfy DOE Order DOE O 231.1B, “Environment, Safety and Health Reporting.” Its purpose is to (1) report compliance status with environmental standards and requirements, (2) present results of environmental monitoring of radiological and nonradiological effluents, (3) report estimated radiological doses to the public from releases of radioactive material, (4) summarize environmental incidents of noncompliance and actions taken in response to them, (5) describe the National Nuclear Security Administration Nevada Field Office (NNSA/NFO) Environmental Management System and characterize its performance, and (6) highlight significant environmental programs and efforts. This NNSSER summarizes data and compliance status for calendar year 2016 at the Nevada National Security Site (NNSS) and its two Nevada-based support facilities, the North Las Vegas Facility (NLVF) and the Remote Sensing Laboratory–Nellis (RSL-Nellis). It also addresses environmental restoration (ER) projects conducted at the Tonopah Test Range (TTR) and the Nevada Test and Training Range (NTTR). NNSA/NFO directs the management and operation of the NNSS and six sites across the nation. In addition to the NNSA itself, the six sites include two in Nevada (NLVF and RSL-Nellis) and four in other states (RSL-Andrews in Maryland, Livermore Operations in California, Los Alamos Operations in New Mexico, and Special Technologies Laboratory in California). Los Alamos, Lawrence Livermore, and Sandia National Laboratories are the principal organizations that sponsor and implement the nuclear weapons programs at the NNSS. National Security Technologies, LLC (NSTec), is the current Management and Operating contractor accountable for the successful execution of work and ensuring that work is performed in compliance with environmental regulations. The six sites all provide support to enhance the NNSS as a location for its multiple missions. The three major NNSS missions include National Security/Defense, Environmental Management, and Nondefense. The major programs that support these missions are Stockpile Stewardship and Management, Nonproliferation and Counterterrorism, Nuclear Emergency Response, Strategic Partnership Projects, Environmental Restoration, Waste Management, Conservation and Renewable Energy, Other Research and Development, and Infrastructure. The major facilities that support the programs include the U1a Facility, Big Explosives Experimental Facility (BEEF), Device Assembly Facility, Dense Plasma Focus Facility, Joint Actinide Shock Physics Experimental Research Facility, Radiological/Nuclear Countermeasures Test and Evaluation Complex, Nonproliferation Test and Evaluation Complex (NPTEC), Radiological/Nuclear Weapons of Mass Destruction Incident Exercise Site, the Area 5 Radioactive Waste Management Complex (RWMC), and the Area 3 Radioactive Waste Management Site (RWMS).

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Nevada National Security Site Environmental Report Summary 2016

This document is a summary of the full 2016 Nevada National Security Site Environmental Report (NNSSER) prepared by the U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/ NFO). This summary provides an abbreviated and more readable version of the full NNSSER. NNSA/NFO prepares the NNSSER to provide the public an understanding of the environmental monitoring and compliance activities that are conducted on the Nevada National Security Site (NNSS) to protect the public and the environment from radiation hazards and from potential nonradiological impacts. It is a comprehensive report of environmental activities performed at the NNSS and offsite facilities over the previous calendar year. The NNSS is currently the nation’s unique site for ongoing national security–related missions and high-risk operations. The NNSS is located about 65 miles northwest of Las Vegas. The approximately 1,360-square-mile site is one of the largest restricted access areas in the United States. It is surrounded by federal installations with strictly controlled access as well as by lands that are open to public entry. In 2016, National Security Technologies, LLC (NSTec), was the NNSS Management and Operations Contractor accountable for ensuring work was performed in compliance with environmental regulations. NNSS activities in 2016 continued to be diverse, with the primary goal to ensure that the existing U.S. stockpile of nuclear weapons remains safe and reliable. Other activities included weapons of mass destruction first responder training; the controlled release of hazardous material at the Nonproliferation Test and Evaluation Complex (NPTEC); remediation of legacy contamination sites; characterization of waste destined for the Waste Isolation Pilot Plant in Carlsbad, New Mexico, or the Idaho National Laboratory in Idaho Falls, Idaho; disposal of low-level and mixed low-level radioactive waste; and environmental research. Facilities and centers that support the National Security/Defense mission include the U1a Facility, Big Explosives Experimental Facility (BEEF), Device Assembly Facility (DAF), National Criticality Experiments Research Center (NCERC) located in the DAF, Joint Actinide Shock Physics Experimental Research (JASPER) Facility, Dense Plasma Focus (DPF) Facility located in the Los Alamos Technical Facility (LATF), and the Radiological/ Nuclear Countermeasures Test and Evaluation Complex (RNCTEC). Facilities that support the Environmental Management mission include the Area 5 Radioactive Waste Management Complex (RWMC) and the Area 3 Radioactive Waste Management Site (RWMS), which has been in cold standby since 2006.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Nevada National Security Site Environmental Report 2014--Summary

This Nevada National Security Site Environmental Report (NNSSER) was prepared to satisfy DOE Order DOE O 231.1B, “Environment, Safety and Health reporting.” Its purpose is to (1) report compliance status with environmental standards and requirements, (2) present results of environmental monitoring of radiological and nonradiological effluents, (3) report estimated radiological doses to the public from releases of radioactive material, (4) summarize environmental incidents of noncompliance and actions taken in response to them, (5) describe the National Nuclear Security Administration Nevada Field Office Environmental (NSA/NFO) Management System and characterize its performance, and (6) highlight significant environmental programs and efforts. The three major NNSS missions include National Security/Defense, Environmental Management, and Nondefense. The major programs that support these missions are Stockpile Stewardship and Management, Nonproliferation and Counterterrorism, Nuclear Emergency Response, Strategic Partnership Project, Environmental Restoration, Waste Management, Conservation and Renewable Energy, Other Research and Development, and Infrastructure. The major facilities that support the programs include the U1a Facility, Big Explosives Experimental Facility (BEEF), Device Assembly Facility, Dense Plasma Focus Facility, Joint Actinide Shock Physics Experimental Research Facility, Radiological/Nuclear Countermeasures Test and Evaluation Complex, Nonproliferation Test and Evaluation Complex (NPTEC), Radiological/Nuclear Weapons of Mass Destruction Incident Exercise Site, Area 5 Radioactive Waste Management Complex (RWMC), and the Area 3 Radioactive Waste Management Site (RWMS).

54 ENVIRONMENTAL SCIENCES↗

Acoustics Examination of Kappa West Firing ARMAG for Personnel Occupancy

In April of 2023, LANL conducted a series of explosive tests at the Kappa West firing mound at BEEF, NNSS, to examine noise levels within the diagnostics ARMAG next to the firing mound. The intent was to establish an explosive mass that could be detonated on the mound without generating blast noise inside the ARMAG which would be unsafe for personnel. The blast noise threshold for personnel safety during intentional firing operations is 140 dB. Based on data collected during prior experiments, it was anticipated that the noise levels inside the ARMAG would be 20-30 dB lower than noise levels outside the ARMAG. However, data from prior experiments were collected from charges that were not consistent geometries and test layouts, so it was unknown what quantity of explosive would generate the threshold noise level. A prediction was made using the BEC-O standard blast calculator from the DDESB, from which it was estimated that a single, consolidated 60-pound charge at 180 feet would generate blast noise levels of about 170 dB, with noise levels falling to a predicted 166 dB at 250 feet.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Organic Waste Resource Assessment for the Detroit Region

This study summarizes major sources of organic wastes in the Detroit region to (1) characterize target feedstock magnitudes and distribution in support of techno-economic analysis (TEA), and (2) guide the design of blended feedstock conversion experiments using hydrothermal liquefaction (HTL). Feedstocks considered in this review include municipal wastewater sludge solids (untreated) and scum; bulk municipal solid waste (MSW); the organic fraction of municipal solid waste (OF-MSW); residential food waste, non-residential food waste including institutional, industrial, and commercial (IIC) sources; confined animal manures (i.e., lactating dairy, feedlot beef, and market swine); waste fats, oils and greases (FOG); agricultural residues; forest residues. The scope of the investigation was limited to existing modeled or publicly available reporting datasets. Bulk MSW data were only collected for context and to generate estimates of OF-MSW by waste type and should not be included in total organic waste estimates. Because the TEA analysis boundary was not defined prior to conducting the resource assessment, the data are summarized within six spatial contexts (boundaries), including (1) city of Detroit (census); (2) Great Lakes Water Authority (GLWA) service area; “Tri-county” urban area (census); “Metro” Detroit-Warren-Dearborn Metropolitan Statistical Area (MSA) (census); Detroit-Warren-Ann Arbor Combined Statistical Area (CSA) (census); and the Michigan Councils of Government (COG) Region-1. All of the spatial contexts are entirely within the State of Michigan, and some overlap one another. A broader context could be developed to include data from surrounding states or Canada.

09 BIOMASS FUELS↗

Enabling Technology - Reducing Greenhouse Gas Emissions and Energy Demands in the Meat Production Industry via Scaling Advanced 3D Culture Bioreactors

The project “Enabling Technology - Reducing Greenhouse Gas Emissions and Energy Demands in the Meat Production Industry via Scaling Advanced 3D Culture Bioreactors” focuses on developing transformational and disruptive food technologies, specifically in the area of alternative protein and meat production techniques. The impetus for developing alternative protein technologies include reducing intensive greenhouse gas emissions and energy/land/water usages associated with conventional meat production (animal agriculture). Additional rationales include providing alternatives to livestock rearing as a means of reducing antimicrobial resistance (most antibiotic and antimicrobial use occurs in food-producing animals), as well as the transmission of zoonotic diseases. The goal of this ARPA-E project was to research and develop novel and effective technologies to produce cell-cultured meat (also named in vitro meat, cultivated meat). Cell-cultured meat is an alternative protein or meat technology that involves directly growing muscle and fat tissues (which comprise meat) without using the full animal. This offers benefits in terms of animal welfare (elimination of animal slaughter), while also being projected to be more resource efficient in terms of metrics such as land use and greenhouse gas emissions, especially when compared to beef and lamb. Cell cultured meat additionally offers greater control over the cultivated cells, enabling more precise control over nutrition and taste. For example, cultured cells could be tuned to produce antioxidants such as carotenoids to improve human health. However, to date there are no scalable methods of producing cell-cultured meat cheaply at a large scale.

59 BASIC BIOLOGICAL SCIENCES↗

Utilizing HYSPLIT for Emergency Response Modeling at SRS

The threat of a disaster happening at the Savannah River Site (SRS) is low, but not zero. When an emergency arises, emergency responders utilize a variety of tools to detect, track, and mitigate the disaster. HYSPLIT is a community atmospheric dispersion model that is highly configurable and has been used operationally to simulate particle, chemical, and radionuclide releases. We have developed a preliminary user interface (UI) that configures HYSPLIT to run for a variety of Emergency Action Level (EAL) scenarios at SRS. Using a hypothetical EAL scenario of an accidental release of Pu-238 in H-area and K-area onsite, an analysis was done to show how a preliminary UI framework allows HYSPLIT to be run on a terminal without the need form an active network connection. The study was successful in multiple aspects. For the preliminary UI, users were able to input certain characteristics of the hypothetical EAL such as hours of emission (how long was Pu-238 being released), curies of release, release start time (when does the model run start), and type of radionuclide directly into the terminal. For HYSPLIT, the model was successful in being able to take the UI input, calculate the results from the inputs and output multiple figures of both total volumetric airborne concentrations and surface deposition. We used the Pu-238 Derived Intervention Level (DIL) deposition thresholds for produce, dairy, and beef for visualization of the surface deposition, showing how a release would impact ingestion pathways.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗

Precursor Analysis Report: JBS Foods Ransomware Attack 2021

The JBS Foods 2021 Ransomware Attack Precursor Analysis Report leverages publicly available information about the JBS ransomware cyber attack and catalogs anomalous observables for each technique employed in the attack. This analysis is based upon the methodology of the Cybersecurity for the Operational Technology Environment (CyOTE) program. In late May 2021, one of the world’s largest meat producers, JBS Foods, announced they had fallen victim to a worldwide ransomware attack, later found to be REvil ransomware. In the United States alone, JBS Foods accounts for nearly 25% of beef and roughly 20% of pork production. Adversaries initially launched a Distributed Denial of Service (DDoS) attack on the company’s Information Technology (IT) networks in Australia, but the attack impacted operations in Brazil, Canada, and the United States, as well. The attack caused plant operations in all four countries to shut down for at least one day. All nine of the U.S. meatpacking plants temporarily shut down because of the attack. The adversaries initially demanded a $\$$22 million ransom for the company’s data, but later negotiated the ransom down to $\$$11 million even after JBS Foods restored most of their systems. JBS Foods eventually paid the $\$$11 Million for reassurance from the adversaries that none of their customers’ data would be compromised in the future. Despite its short duration, the attack still caused large stocks of meat to spoil. The incident also underscored how adversaries can simultaneously compromise and move laterally through global subsidiaries of an organization. Researchers and analysts identified 22 unique techniques (in a sequence of 21 steps) utilized during the attack with a total of 361 observables using MITRE ATT&CK® for Industrial Control Systems. The CyOTE program assesses observables accompanying techniques used prior to the triggering event to identify opportunities to detect malicious activity. If observables accompanying the attack techniques are perceived and investigated prior to the triggering event, earlier comprehension of malicious activity can take place. Sixteen of the identified techniques used during the JBS Foods cyber attack were precursors to the triggering event. Analysis identified 308 observables associated with these precursor techniques, 163 of which were assessed to have an increased likelihood of being perceived in the 75 days preceding the triggering event. The response and comprehension time could have been reduced if the observables had been identified earlier. The information gathered in this report contributes to a library of observables tied to a repository of artifacts, data sources, and technique detection references for practitioners and developers to support the comprehension of indicators of attack. Asset owners and operators can use these products if they experience similar observables or to prepare for comparable scenarios.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

A Physical Model for Understanding the Activation of MoS 2 Basal-plane Sulfur Atoms for the Hydrogen Evolution Reaction

All the DFT calculations are done with the Vienna Ab Initio Simulation Package (VASP) using the projector augmented wave method. The Bayesian error estimation exchange-correlation functionals (BEEF) with van der Waals interactions are employed. A plane-wave cutoff energy of 400 eV is used together with PAW-PBE potentials where semi core p states are treated as valence. All the calculations allow for spin-polarization. The structures are relaxed until the force is converged to < 0.01 eV/Å. The lattice parameter of MoS 2 unit cell, optimized with this functional, is 3.19 Å. A (4×4) supercell is used to model all the transition metal doped MoS 2 systems studied here, including those with S vacancies. For calculations in the initial dopant structure exploration, the Brillouin zone is sampled with a 3x3x1 Monkhorst-Pack k-point mesh. A 6×6×1 Monkhorst-Pack k-point mesh is used for the H binding energy and density of states calculations. In all calculations, the vacuum layer is set as 15 Å to eliminate periodic interaction perpendicular to the basal plane.

77 NANOSCIENCE AND NANOTECHNOLOGY↗

Epidemiologic and economic considerations regarding persistently infected cattle during vaccinate-to-live strategies for control of foot-and-mouth disease in FMD-free regions

Development of a foot-and-mouth disease (FMD) carrier state following FMD virus (FMDV) infection is a well-established phenomenon in cattle. However, the proportion of cattle likely to become carriers and the duration of the carrier state at a herd or population-level are incompletely understood. The objective of this study was to examine the epidemiologic and economic impacts of vaccination-to-live strategy in a disease-free region or country. We developed and simulated scenarios of FMD spread and control in the US livestock population, which included depopulation for a limited period, followed by a vaccinate-to-live strategy with strong biosecurity and movement restrictions. Six scenarios of FMD spread and control were simulated in the InterSpread Plus (ISP) modeling tool. Data on the number of infected and depopulated cattle (by operation types) from ISP model runs were used to estimate the monthly number of infected but not depopulated (potential carrier) cattle after the infection. Using available literature data on the FMD carrier state, we estimated the monthly proportion of carrier cattle (from infected but not depopulated cattle) over time following infection. Among the simulated scenarios, the median (25th, 75th percentile) number of infected cattle ranged from 43,217 (42,819, 55,274) head to 148,907 (75,819, 205,350) head, and the epidemic duration ranged from 20 (11, 30) to 76 (38, 136) days. In general, larger outbreaks occurred when depopulation was carried out through longer periods, and the onset of the vaccination was late (p > 0.05). The estimated proportion of surviving cattle, which were infected and not depopulated and had the potential to become persistently infected ranged from 14 to 35% of total infected cattle. Production losses in beef and dairy sectors were higher when outbreaks started in multiple states simultaneously, but production losses were small compared to trade losses and consumer avoidance losses. These results can be used to inform the consideration of a vaccinate-to-live strategy for FMD outbreaks and the development of appropriate post-outbreak management strategies. Furthermore, this output will enable a more detailed examination of the epidemiologic and economic implications of allowing convalescent cattle to survive and remain in production chains after FMD outbreaks in FMD-free regions.

60 APPLIED LIFE SCIENCES↗

Aircraft-based inversions quantify the importance of wetlands and livestock for Upper Midwest methane emissions

Abstract. We apply airborne measurements across three seasons (summer, winter and spring 2017–2018) in a multi-inversion framework to quantify methane emissions from the US Corn Belt and Upper Midwest, a key agricultural and wetland source region. Combing our seasonal results with prior fall values we find that wetlands are the largest regional methane source (32 %, 20 [16–23] Gg/d), while livestock (enteric/manure; 25 %, 15 [14–17] Gg/d) are the largest anthropogenic source. Natural gas/petroleum, waste/landfills, and coal mines collectively make up the remainder. Optimized fluxes improve model agreement with independent datasets within and beyond the study timeframe. Inversions reveal coherent and seasonally dependent spatial errors in the WetCHARTs ensemble mean wetland emissions, with an underestimate for the Prairie Pothole region but an overestimate for Great Lakes coastal wetlands. Wetland extent and emission temperature dependence have the largest influence on prediction accuracy; better representation of coupled soil temperature–hydrology effects is therefore needed. Our optimized regional livestock emissions agree well with the Gridded EPA estimates during spring (to within 7 %) but are ∼ 25 % higher during summer and winter. Spatial analysis further shows good top-down and bottom-up agreement for beef facilities (with mainly enteric emissions) but larger (∼ 30 %) seasonal discrepancies for dairies and hog farms (with > 40 % manure emissions). Findings thus support bottom-up enteric emission estimates but suggest errors for manure; we propose that the latter reflects inadequate treatment of management factors including field application. Overall, our results confirm the importance of intensive animal agriculture for regional methane emissions, implying substantial mitigation opportunities through improved management.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Detection of a lysosomal carboxypeptidase and a lysosomal dipeptidase in highly-purified dipeptidyl aminopeptidase I /cathepsin C/ and the elimination of their activities from preparations used to sequence peptides.

Description of the properties of a carboxypeptidase, termed 'catheptic carboxypeptidase C,' and a dipeptidase, termed 'Ser-Met dipeptidase.' Both were found in highly purified DAP I from either beef spleen or rat liver. Methods are described for the removal or selective inactivation of these contaminating enzymes.

Mcdonald, J. K.↗

Study of blood flow sensing with microwave radiometry

A study and experimental investigation has been performed to determine the feasibility of measuring regional blood flow and volume in man by means of microwave radiometry. An indication was expected of regional blood flow from measurement of surface and subsurface temperatures with a sensitive radiometer. Following theoretical modeling of biological tissue, to determine the optimum operating frequency for adequate sensing depth, a sensitive microwave radiometer was designed for operation at 793 MHz. A temperature sensitivity of of 0.06 K rms was realized in this equipment. Measurements performed on phantom tissue models, consisting of beef fat and lean beefsteak showed that the radiometer was capable of sensing temperatures from a depth between 3.8 and 5.1 cm. Radiometric and thermodynamic temperature measurements were also performed on the hind thighs of large dogs. These showed that the radiometer could sense subsurface temperatures from a depth of, at least, 1.3 cm. Delays caused by externally-generated RF interference, coupled with the lack of reliable blood flow measurement equipment, prevented correlation of radiometer readings with reginal blood flow. For the same reasons, it was not possible to extend the radiometric observations to human subjects.

Porter, R. A.↗

Monitoring the vernal advancement and retrogradation (green wave effect) of natural vegetation

The author has identified the following significant results. Emphasis has been given to an inventory of land resource types and land use at the ten Great Plains Corridor test sites. A resource and land use classification system was developed which uses available soil survey information and interpretations from NASA obtained high flight aerial photography to locate discrete areas of similar rangeland vegetation. Existing classification systems, even those developed for use with remote sensor data, were found to be inadequate for this project. This system is expected to be of general use for remote sensing related to land use and management. It has specific applicability to any effort aimed at regional use of ERTS-1 MSS digital data products. A preliminary assessment of the relative importance of rangelands in the Great Plains Corridor states indicates that the value of the livestock industry supported by this resource exceeds 23 billion dollars. The development of a Rangeland Feed Conditions index for this region could be used by more than 400,000 farm and ranch operators involved in the production of more than 40% of the nation's beef and much of the country's grain.

Rouse, J. W., Jr.↗

Land use mapping in Erie County, Pennsylvania: A pilot study

The author has identified the following significant results. A pilot study was conducted to determine the feasibility of mapping land use in the Great Lakes Basin area utilizing ERTS-1 data. Small streams were clearly defined by the presence of trees along their length in predominantly agricultural country. Field patterns were easily differentiated from forested areas; dairy and beef farms were differentiated from other farmlands, but no attempt was made to identify crops. Large railroad lines and major highway systems were identified. The city of Erie and several smaller towns were identified, as well as residential areas between these towns, and docks along the shoreline in Erie. Marshes, forests, and beaches within Presque Isle State Park were correctly identified, using the DCLUS program. Bay water was differentiated from lake water, with a small amount of misclassification.

Mcmurtry, G. J.↗