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At least 883 records · Page 49
All sourcesNASA NTRSDOE OSTI
NASA NTRS2000

BL-Lacs in X-Ray Outburst

We conducted a multifrequency campaign for the TeV blazar Markarian 421 in 1998 April. The campaign started from a pronounced high-amplitude flare recorded by BeppoSAX and Whipple; the Advanced Satellite for Cosmology and Astrophysics (ASCA) observation started three days later. In the X-ray data, we detected multiple flares, occurring on timescales of about one day. ASCA data clearly reveal spectral variability. The comparison of the data from ASCA, the Extreme Ultraviolet Explorer, and the Rossi X-Ray Timing Explorer indicates that the variability amplitudes in the low-energy synchrotron component are larger at higher photon energies. In TeV and gamma-rays, large intraday variations-which were correlated with the X-ray flux-were observed when results from three Cerenkov telescopes were combined. The rms variability of TeV and gamma-rays was similar to that observed in hard X-rays, above ten keV. The X-ray light curve reveals flares that are almost symmetric for most cases, implying that the dominant timescale is the light crossing time through the emitting region. The structure function analysis based on the continuous X-ray light curve of seven days indicates that the characteristic timescale is approx. 0.5 days. The analysis of ASCA light curves in various energy bands appears to show both soft (positive) and hard (negative) lags. These may not be real, as systematic effects could also produce these lags, which are all much smaller than an orbit. If the lags of both signs are real, these imply that the particle acceleration and X-ray cooling timescales are similar.

Remillard, Ronald A.↗
NASA NTRS2000

Comment on "Heterodyne Lidar Returns in the Turbulent Atmosphere: Performance Evaluation of Simulated Systems"

The explanation for the difference between simulation and the zero-order theory for heterodyne lidar returns in a turbulent atmosphere proposed by Belmonte and Rye is incorrect. The theoretical expansion is not developed under a square- law-structure function approximation (random wedge atmosphere). Agreement between the simulations and the zero-order term of the theoretical expansion is produced for the limit of statistically independent paths (bi-static operation with large transmitter-receiver separation) when the simulations correctly include the large-scale gradients of the turbulent atmosphere.

Frehlich, Rod↗
NASA NTRS2000

Reduced Gas Cycling in Microbial Mats: Implications for Early Earth

For more than half the history of life on Earth, biological productivity was dominated by photosynthetic microbial mats. During this time, mats served as the preeminent biological influence on earth's surface and atmospheric chemistry and also as the primary crucible for microbial evolution. We find that modern analogs of these ancient mat communities generate substantial quantities of hydrogen, carbon monoxide, and methane. Escape of these gases from the biosphere would contribute strongly to atmospheric evolution and potentially to the net oxidation of earth's surface; sequestration within the biosphere carries equally important implications for the structure, function, and evolution of anaerobic microbial communities within the context of mat biology.

Hoehler, Tori M.↗
NASA NTRS2002

Probing the Masses of the PSR JO621+1002 Binary System Through Relativistic Apsidal Motion

Orbital, spin and astrometric parameters of the millisecond pulsar PSR J0621+1002 have been determined through six years of timing observations at three radio telescopes. The chief result is a measurement of the rate of periastron advance, omega=0 deg.0116 +/-0 deg.0008/yr. Interpreted as a general relativistic effect, this implies the sum of the pulsar mass, m(1), and the companion mass, m(2), to be M=m(1)+m(2)= 2.81 +/-0.30 solar mass. The Keplerian parameters rule out certain combinations of m(1) and m(2), as does the non-detection of Shapiro delay in the pulse arrival times. These constraints, together with the assumption that the companion is a white dwarf, lead to the maximum likelihood values m(1)=1.69((sup +0.30)(sub -0.30)) solar mass and m(2)=0.98((sup +0.32)(sub -0.12) solar mass (68% confidence). The other major finding is that the pulsar experiences dramatic variability in its dispersion measure (DM), with gradients as steep as 0.013 pc/cu cm/yr. A structure function analysis of the DM variations uncovers spatial fluctuations in the interstellar electron density that cannot be fit to a single power law, unlike the Kolmogorov turbulent spectrum that has been seen in the direction of other pulsars. Other results from the timing analysis include the first measurements of the pulsar's proper motion, mu=3.5+/-0.3 mas/yr, and of its spin-down rate, dP/dt=4.7 x 10(exp -20), which, when corrected for kinematic biases and combined with the pulse period, P=28.8 ms, gives a characteristic age of 1.1 x 10(exp 10) yr and a surface magnetic field strength of 1.2 x 10 (exp 9) G.

Spaver, Eric M.↗
NASA NTRS2002

Peptides at Membrane Surfaces and their Role in the Origin of Life

All ancestors of contemporary cells (protocells) had to transport ions and organic matter across membranous walls, capture and utilize energy and transduce environmental signals. In modern organisms, all these functions are preformed by membrane proteins. We make the parsimonious assumption that in the protobiological milieu the same functions were carried out by their simple analogs - peptides. This, however, required that simple peptides could self-organize into ordered, functional structures. In a series of detailed, molecular-level computer simulations we demonstrated how this is possible. One example is the peptide (LSLLLSL)3 which forms a trameric bundle capable of transporting protons across membranes. Another example is the transmembrane pore of the influenza M2 protein. This aggregate of four identical alpha-helices, each built of 25 amino acids, forms an efficient and selective voltage-gated proton channel. Our simulations explain the gating mechanism in this channel. The channel can be re-engineered into a simple proton pump.

Pohorille, Andrew↗
NASA NTRS2003

The Neptune/Triton Explorer Mission: A Concept Feasibility Study

Technological advances over the next 10 to 15 years promise to enable a number of smaller, more capable science missions to the outer planets. With the inception of miniaturized spacecraft for a wide range of applications, both in large clusters around Earth, and for deep space missions, NASA is currently in the process of redefining the way science is being gathered. Technologies such as 3-Dimensional Multi-Chip Modules, Micro-machined Electromechanical Devices, Multi Functional Structures, miniaturized transponders, miniaturized propulsion systems, variable emissivity thermal coatings, and artificial intelligence systems are currently in research and development, and are scheduled to fly (or have flown) in a number of missions. This study will leverage on these and other technologies in the design of a lightweight Neptune orbiter unlike any other that has been proposed to date. The Neptune/Triton Explorer (NExTEP) spacecraft uses solar electric earth gravity assist and aero capture maneuvers to achieve its intended target orbit. Either a Taurus or Delta-class launch vehicle may be used to accomplish the mission.

Esper, Jaime↗
NASA NTRS2001

Characteristic Ultraviolet/Optical Timescales in Active Galactic Nuclei

The UV/optical light curves of active galactic nuclei (AGNs) may be roughly characterized by a periodic fractional flux variations of approx. 10% on timescales of 1 month. The physical mechanism(s) responsible remain ill defined. We present a structure function analysis, i.e., measure the power distribution over a range of timescales tau, of 13 AGNs to constrain the origin of UV/optical emission. On timescales tau approx. 5- 60 days, the mean UV and optical power density spectra (PDS) are equivalent. This may suggest that the underlying energy generating mechanism is identical. The combined W/optical PDS is P(f) proportional to f(sup -alpha)- with alpha = 2.13(sup +0.22, sub -0.06). For sources with measured X-ray PDS indices, we find they are indistinguishable from their UV/optical counterparts. This supports scenarios whereby X-rays are generated via Compton upscattering of UV photons, to later radiatively drive optical variations. At the same time, we present evidence for characteristic variability timescales tau(sub char) of approx. 5-100 days in 10 sources. These variability timescales combined with reverberation based masses M suggest a M - tau(sub char) relationship; higher mass systems have larger characteristic timescales. The UV tau(sub char) may possibly reflect dynamical or accretion disk thermal timescales. We find suggestive evidence for a dichotomy, at tau approx. 30 days and M approx. l0(exp 7) solar mass, between short- and long-time scale optical variations. These optical variations may be attributable to dynamical and accretion disk thermal or starburst activity timescales, respectively.

Collier, Stefan↗
NASA NTRS2001

The interaction of microgravity and ethylene on the ultrastructure cell and Ca2+ localization in soybean hook hypocotyl

Calcium ions are secondary messenger in numerous cellular processes of plant grown at 1 g. Ca2+ are connected with oxygen atoms, of pectin carboxy groups and/or with H(+)-groups of protein (Roux and Slocum, 1982; Hepler and Wayne, 1985). The influence of altered gravity on the calcium balance in some cells is established. The increased synthesis of ethylene in plant grown in microgravity caused the change of the structural-functional organization of cell (Hensel and Iversen, 1980; Hilaire et al., 1996). Available data put the new question: how do high ethylene level and microgravity influence on the redistribution of Ca2+ in cell of seedling in early stage of growth? Therefore, the goal of our data was the comparable study of the cell ulltrastructure and localization of Ca2+ in hook hypocotyl of soybean seedling under interaction of microgravity and ethylene.

STS-87 Shuttle Project↗
NASA NTRS2003

Hindlimb unloading alters ligament healing

We investigated the hypothesis that hindlimb unloading inhibits healing in fibrous connective tissue such as ligament. Male rats were assigned to 3- and 7-wk treatment groups with three subgroups each: sham control, ambulatory healing, and hindlimb-suspended healing. Ambulatory and suspended animals underwent surgical rupture of their medial collateral ligaments, whereas sham surgeries were performed on control animals. After 3 or 7 wk, mechanical and/or morphological properties were measured in ligament, muscle, and bone. During mechanical testing, most suspended ligaments failed in the scar region, indicating the greatest impairment was to ligament and not to bone-ligament insertion. Ligament testing revealed significant reductions in maximum force, ultimate stress, elastic modulus, and low-load properties in suspended animals. In addition, femoral mineral density, femoral strength, gastrocnemius mass, and tibialis anterior mass were significantly reduced. Microscopy revealed abnormal scar formation and cell distribution in suspended ligaments with extracellular matrix discontinuities and voids between misaligned, but well-formed, collagen fiber bundles. Hence, stress levels from ambulation appear unnecessary for formation of fiber bundles yet required for collagen to form structurally competent continuous fibers. Results support our hypothesis that hindlimb unloading impairs healing of fibrous connective tissue. In addition, this study provides compelling morphological evidence explaining the altered structure-function relationship in load-deprived healing connective tissue.

NASA Discipline Musculoskeletal↗
NASA NTRS2001

'The surface management system' (SuMS) database: a surface-based database to aid cortical surface reconstruction, visualization and analysis

Surface reconstructions of the cerebral cortex are increasingly widely used in the analysis and visualization of cortical structure, function and connectivity. From a neuroinformatics perspective, dealing with surface-related data poses a number of challenges. These include the multiplicity of configurations in which surfaces are routinely viewed (e.g. inflated maps, spheres and flat maps), plus the diversity of experimental data that can be represented on any given surface. To address these challenges, we have developed a surface management system (SuMS) that allows automated storage and retrieval of complex surface-related datasets. SuMS provides a systematic framework for the classification, storage and retrieval of many types of surface-related data and associated volume data. Within this classification framework, it serves as a version-control system capable of handling large numbers of surface and volume datasets. With built-in database management system support, SuMS provides rapid search and retrieval capabilities across all the datasets, while also incorporating multiple security levels to regulate access. SuMS is implemented in Java and can be accessed via a Web interface (WebSuMS) or using downloaded client software. Thus, SuMS is well positioned to act as a multiplatform, multi-user 'surface request broker' for the neuroscience community.

NASA Discipline Neuroscience↗
NASA NTRS1999

Protein crystal growth and the International Space Station

Protein structural information plays a key role in understanding biological structure-function relationships and in the development of new pharmaceuticals for both chronic and infectious diseases. The Center for Macromolecular Crystallography (CMC) has devoted considerable effort studying the fundamental processes involved in macromolecular crystal growth both in a 1-g and microgravity environment. Results from experiments performed on more than 35 U.S. space shuttle flights have clearly indicated that microgravity can provide a beneficial environment for macromolecular crystal growth. This research has led to the development of a new generation of pharmaceuticals that are currently in preclinical or clinical trials for diseases such as cutaneous T-cell lymphoma, psoriasis, rheumatoid arthritis, AIDS, influenza, stroke and other cardiovascular complications. The International Space Station (ISS) provides an opportunity to have complete crystallographic capability on orbit, which was previously not possible with the space shuttle orbiter. As envisioned, the x-ray Crystallography Facility (XCF) will be a complete facility for growing protein crystals; selecting, harvesting, and mounting sample crystals for x-ray diffraction; cryo-freezing mounted crystals if necessary; performing x-ray diffraction studies; and downlinking the data for use by crystallographers on the ground. Other advantages of such a facility include crystal characterization so that iterations in the crystal growth conditions can be made, thereby optimizing the final crystals produced in a three month interval on the ISS.

STS Shuttle Project↗
NASA NTRS1999

Phosphoinositide kinases and the synthesis of polyphosphoinositides in higher plant cells

Phosphoinositides are a family of inositol-containing phospholipids which are present in all eukaryotic cells. Although in most cells these lipids, with the exception of phosphatidylinositol, constitute only a very minor proportion of total cellular lipids, they have received immense attention by researchers in the past 15-20 years. This is due to the discovery that these lipids, rather than just having structural functions, play key roles in a wide range of important cellular processes. Much less is known about the plant phosphoinositides than about their mammalian counterparts. However, it has been established that a functional phosphoinositide system exists in plant cells and it is becoming increasingly clear that inositol-containing lipids are likely to play many important roles throughout the life of a plant. It is not our intention to give an exhaustive overview of all aspects of the field, but rather we focus on the phosphoinositide kinases responsible for the synthesis of all phosphorylated forms of phosphatidylinositol. Also, we mention some of the aspects of current phosphoinositide research which, in our opinion, are most likely to provide a suitable starting point for further research into the role of phosphoinositides in plants.

Review↗
NASA NTRS1998

Calcium signals and calcium channels in osteoblastic cells

Calcium (Ca2+) channels are present in non-excitable as well as in excitable cells. In bone cells of the osteoblast lineage, Ca2+ channels play fundamental roles in cellular responses to external stimuli including both mechanical forces and hormonal signals. They are also proposed to modulate paracrine signaling between bone-forming osteoblasts and bone-resorbing osteoclasts at local sites of bone remodeling. Calcium signals are characterized by transient increases in intracellular Ca2+ levels that are associated with activation of intracellular signaling pathways that control cell behavior and phenotype, including patterns of gene expression. Development of Ca2+ signals is a tightly regulated cellular process that involves the concerted actions of plasma membrane and intracellular Ca2+ channels, along with Ca2+ pumps and exchangers. This review summarizes the current state of knowledge concerning the structure, function, and role of Ca2+ channels and Ca2+ signals in bone cells, focusing on the osteoblast.

Non-NASA Center↗
NASA NTRS2006

Water as a matrix for life

"Follow the water" is the canonical strategy in searching for life in the universe. Conventionally, discussion of this topic is focused on how solvent supports organic chemistry sufficiently rich to seed life. Perhaps more importantly, solvent must promote self-organization of organic matter into functional structures capable of responding to environmental changes. This process is based on non-covalent interactions. They are constantly formed and broken in response to internal and external stimuli. This requires that their strength must be properly tuned. If they were too weak, the system would exhibit undesired, uncontrolled response to natural fluctuations of physical and chemical parameters. If they were too strong kinetics of biological processes would be slow and energetics costly. Non-covalent interactions are strongly mediated by the solvent. Specifically, high dielectric solvents for life are needed for solubility of polar species and flexibility of biological structures stabilized by electrostatic interactions. Water exhibits a remarkable trait that it promotes solvophobic interactions between non-polar species, which are responsible for self-organization phenomena such as the formation of cellular boundary structures, and protein folding and aggregation. Unusual temperature dependence of hydrophobic interactions - they often become stronger as temperature increases - is a consequence of the temperature insensitivity of properties of the liquid water. This contributes to the existence of robust life over a wide temperature range. Water is not the only liquid with favorable properties for supporting life. Other pure liquids or their mixtures that have high dielectric constants and simultaneously support some level of self-organization will be discussed.

Pohorille, Andrew↗
NASA NTRS1994

(abstract) A VLBI Test of Tropospheric Delay Calibration with WVRs

Dual frequency (S/X band) very long baseline interferometry (VLBI) observations were used to test troposphere calibration by water vapor radiometers (WVRs). Comparison of the VLBI and WVR measurements show a statistical agreement (specifically, their structure functions agree) on time scales less than 700 seconds. On longer time scales, VLBI instrumental errors become important. The improvement in VLBI residual delays from WVR calibration was consistent with the measured level of tropospheric fluctuations.

VLBI water vapor radiometers troposphere interfero↗
NASA NTRS2007

Quantifiable Assessment of SWNT Dispersion in Polymer Composites

NASA LaRC has established a new protocol for visualizing the nanomaterials in structural polymer matrix resins. Using this new technique and reconstructing the 3D distribution of the nanomaterials allows us to compare this distribution against a theoretically perfect distribution. Additional tertiary structural information can now be obtained and quantified with the electron tomography studies. These tools will be necessary to establish the structural-functional relationships between the nano and the bulk. This will also help define the critical length scales needed for functional properties. Field ready tool development and calibration can begin by using these same samples and comparing the response. i.e. gold standards of good and bad dispersion.

Park, Cheol↗
NASA NTRS2005

Micro-Inspector Spacecraft for Space Exploration Missions

NASA is seeking to embark on a new set of human and robotic exploration missions back to the Moon, to Mars, and destinations beyond. Key strategic technical challenges will need to be addressed to realize this new vision for space exploration, including improvements in safety and reliability to improve robustness of space operations. Under sponsorship by NASA's Exploration Systems Mission, the Jet Propulsion Laboratory (JPL), together with its partners in government (NASA Johnson Space Center) and industry (Boeing, Vacco Industries, Ashwin-Ushas Inc.) is developing an ultra-low mass (<3.0 kg) free-flying micro-inspector spacecraft in an effort to enhance safety and reduce risk in future human and exploration missions. The micro-inspector will provide remote vehicle inspections to ensure safety and reliability, or to provide monitoring of in-space assembly. The micro-inspector spacecraft represents an inherently modular system addition that can improve safety and support multiple host vehicles in multiple applications. On human missions, it may help extend the reach of human explorers, decreasing human EVA time to reduce mission cost and risk. The micro-inspector development is the continuation of an effort begun under NASA's Office of Aerospace Technology Enabling Concepts and Technology (ECT) program. The micro-inspector uses miniaturized celestial sensors; relies on a combination of solar power and batteries (allowing for unlimited operation in the sun and up to 4 hours in the shade); utilizes a low-pressure, low-leakage liquid butane propellant system for added safety; and includes multi-functional structure for high system-level integration and miniaturization. Versions of this system to be designed and developed under the H&RT program will include additional capabilities for on-board, vision-based navigation, spacecraft inspection, and collision avoidance, and will be demonstrated in a ground-based, space-related environment. These features make the micro-inspector design unique in its ability to serve crewed as well as robotic spacecraft, well beyond Earth-orbit and into arenas such as robotic missions, where human teleoperation capability is not locally available.

microspacecraft↗