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At least 883 records · Page 49

Contact and pressure balance structures in two-fluid cosmic-ray hydrodynamics

The role of cosmic-ray-modified contact discontinuities and pressure balance structures in two-fluid cosmic-ray hydrodynamics in one Cartesian space dimension are investigated by means of analytic and numerical solution examples, as well as by weakly nonlinear asymptotics. The fundamental wave modes of the two-fluid cosmic-ray hydrodynamic equations in the long-wavelength limit consist of the backward and forward propagating cosmic-ray-modified sound waves, with sound speed dependent on both the cosmic-ray and thermal gas pressures; the contact discontinuity; and a pressure balance mode in which the sum ofthe cosmic ray and thermal gas pressure perturbations is zero. The pressure balance mode, like the contact discontinuity is advected with the background flow. The interaction of the pressure balance mode with the contact discontinuity is investigated by means of the method of multiple scales. The thermal gas and cosmic-ray pressure perturbations satisfy a linear diffusion equation, and entropy perturbations arising from nonisentropic initial conditions for the thermal gas are frozen into the fluid. The contact discontinuity and pressure balance eigenmodes both admit nonzero perturbations in the thermal gas, whereas the cosmic-ray-modified sound waves are isentropic. The total entropy perturbation is shared between the contact discontinuity and pressure balance eigenmodes, and examples are given in which there is a transfer of entropy between the two modes. In particular, N-wave type density disturbances are obtained which arise as a result of the entropy transfer between the two modes. A weakly nonlinear geometric optics perturbation expansion is used to study the long timescale evolution of the short-wavelength entropy wave and the thermal gas sound waves in a slowly varying, large-scale background flow. The weakly nonlinear geometric optics expansion is also used to generalize previous studies of squeezing instability for short-wavelength sound waves in the two fluid model, by including a weakly nonlinear wave steepening term that leads to shock formation, as well as the effect of long time and space dependence of the background flow. Implications of cosmic-ray-modified pressure balance structures and contact discontinuities in models of the interaction of traveling interplanetary shocks and compression and rarefraction waves with the solar wind termination shock are briefly discussed.

Webb, G. M.↗

Stability and convergence of nuclear detonations in white dwarf collisions

We investigate the numerical stability of thermonuclear detonations in 1D accelerated reactive shocks and 2D binary collisions of equal-mass magnetized and unmagnetized white dwarf stars. To achieve high resolution at initiation sites, we devised geometric gridding and mesh velocity strategies specially adapted to the unique requirements of head-on collisional geometries, scenarios in which one expects maximum production of iron-group products. We study the effects of grid resolution and the limiting of temperature, energy, and reactants for different stellar masses, separations, magnetic fields, inigenerationtial compositions, detonation mechanisms, and limiter parameters across a range of cell sizes from 1 to 100 km. Our results set bounds on the parameter space of limiter amplitudes for which both temperature- and energy-limiting procedures yield consistent and monotonically convergent solutions. Within these bounds, we find that grid resolutions of 5 km or better are necessary for uncertainties in total released energy and iron-group products to drop below 10%. Intermediate-mass products (e.g., calcium) exhibit similar convergence trends but with somewhat greater uncertainty. These conclusions apply equally to pure C/O white dwarfs, multispecies compositions (including helium shells), magnetized and unmagnetized cores, and either single or multiple detonation scenarios.

79 ASTRONOMY AND ASTROPHYSICS↗

Constraints on Mechanisms of Chondrule Formation from Chondrule Precursors and Chronology of Transient Heating Events in the Protoplanetary Disk

The mineralogy, petrography, and oxygen-isotope compositions of porphyritic chondrules—dominant chondrule type in most chondrite groups—suggest formation by incomplete melting of isotopically diverse precursors during localized transient heating events in dust-rich regions of the protoplanetary disk characterized by 16O-poor compositions (D17Odust+gas~ –7‰ to +4‰) relative to the inferred Sun’s value (D17O ~ –28 ± 2‰). The chondrule precursors included Ca,Al-rich inclusions (CAIs), amoeboid olivine aggregates (AOAs), chondrules of earlier generations, fine-grained matrix-like material, and possibly fragments of pre-existing planetesimals. Like porphyritic chondrules, igneous CAIs formed by melting of isotopically diverse precursors during transient heating events, but in an isotopically distinct, solar-like reservoir of the protoplanetary disk (D17O dust + gas ~ –24‰), probably near the proto Sun. Based on a narrow range of the initial 26Al/27Alratios inferred from the internal Al-Mg isochrons in igneous CAIs, their melting started at the very beginning of Solar System formation (t0), defined by the CV CAIs with U-corrected Pb-Pb age of 4567.3 ± 0.16 Ma and the canonical 26Al/27Al ratio of (5.25 ± 0.02) ¥ 10–5, and lasted at least 0.3 Ma. The U-corrected Pb-Pb absolute and 26Al-26Mg relative ages of porphyritic chondrules from type 3 ordinary, CO, CV, and CR carbonaceous chondrites (assuming uniform distribution of 26Al in the disk at the canonical level) suggest chondrule formation started at t0 and lasted for about 4 Ma. These observations may preclude formation of the majority of porphyritic chondrules by splashing of differentiated planetesimals and by collisions between planetesimals; instead, they are consistent with melting of dust balls by bow shocks or magnetized turbulence in the disk. Some porphyritic chondrules in equilibrated (petrologic type 4–6) ordinary chondrites contain relict fragments of coarse-grained chromite, ilmenite, phosphates, and albitic plagioclase. The similar mineral assemblage is commonly observed in type 4–6 ordinary chondrites, but is absent in type 3 chondrites, suggesting these chondrules formed by incomplete melting of thermally metamorphosed ordinary chondrite material, possibly by impacts. The CB metal-rich carbonaceous chondrites contain exclusively magnesian non-porphyritic chondrules crystallized from complete melts. These chondrules formed in a gas-melt plume generated by a hypervelocity (≥20 km/s) collision between planetesimals ~4.8 Ma after t0 in a transition or a debris disk. One of the colliding bodies was probably differentiated. The CH metal-rich carbonaceous chondrites contain chondrules formed by different mechanisms. The magnesian non-porphyritic chondrules formed in the CB impact plume ~4.8 Ma after t0. The chemically diverse (magnesian, ferroan, and Al-rich) porphyritic chondrules formed by incomplete melting of isotopically diverse precursors in the protoplanetary disk, most likely prior the CB impact plume event. We conclude that there are multiple mechanisms of chondrule formation that operated over the entire life-time of the disk.

Chondrules↗

ALMA Observations of Vibrationally Excited HC{sub 3}N Lines Toward Orion KL

We present high spatial resolution ALMA observations of vibrational transitions of HC{sub 3}N toward Orion KL in the 214–247 GHz frequency band. 41 transitions of HC{sub 3}N in 7 vibrationally excited states, and 23 transitions of {sup 13}C isotopologues of HC{sub 3}N in 2 vibrational states are detected. The line images show that vibrationally excited HC{sub 3}N lines originate mainly from the hot core of Orion and IRc7. The images of HC{sub 3}N vibrationally excited lines show that the line emission peaks associated with the hot core move from south to northeast as E{sub u} increases. Based on multiple transitions of each vibrationally excited state, we performed local thermodynamic equilibrium calculations in the XCLASS suite toward the hot core and IRc7 positions. Generally, transitions in highly excited states have higher rotational temperatures and lower column densities. The rotational temperatures and column densities of the hot core range from 93 to 321 K, and from 1.0×10{sup 14} to 4.9×10{sup 16} cm{sup −2}, respectively. Lower rotational temperatures ranging from 88 to 186 K and column densities from 1.0×10{sup 14} to 3.2×10{sup 16} cm{sup −2} are obtained toward IRc7. The facts that the hot core emission peaks of vibrationally excited HC{sub 3}N lines move from south to northeast with increasing E{sub u}, and that higher-energy HC{sub 3}N lines have higher rotational temperatures and lower column densities, appear to support that the hot core is externally heated. The emission peaks are moving along the major axis of the SiO outflow, which may indicate that higher-energy HC{sub 3}N transitions are excited by interaction between pre-existing dense medium and shocks generated by SiO outflows.

79 ASTRONOMY AND ASTROPHYSICS↗

Experimental Impacts into Chondritic Targets: Disruption of an L6 Chondrite by Multiple Impacts - Part 1

A fragment of an L6 chondrite (ALH 85017,13) with an initial mass (M(sub 0)) of 464.1 g was the target in a series of experimental impacts in which the largest remaining fragment (M(sub R)) after each shot was impacted by a 3.18-mm ceramic sphere at a nominal speed of 2 km/s. This continued until the mass of the largest remaining piece was less than half the mass of the target presented to that shot (M(sub S)). Two chunks of Bushveldt gabbro with similar initial masses were also impacted under the same conditions until M(sub R) was less than half M(sub 0). The two gabbro targets required a total of 1.51x10(exp 7) and 1.75x10(exp 7) erg/g to attain 0.27 and 0.33 M(sub R)/M(sub 0), respectively; the chondrite, however, was considerably tougher, reaching 0.40 and 0.21 M(sub R)/M(sub 0) only after receiving 2.37x10(exp 7) and 3.10x10(exp 7) erg g-1, respectively. The combined ejecta and spallation products from the gabbro impacts were coarser than those from the chondrite and in sufficient quantities that the new surface areas exceeded those from the meteorite until the fifth shot in the chondrite series, which was the number of impacts required to disrupt each gabbro target (i.e., MR/M0 = 0.5). Unlike the behavior shown in previous regolith-evolution series, neither gabbro target produced an enhancement in the size fraction reflecting the mean size of the crystals composing the rock (about 3 mm), an effect possibly related to the width of the shock pulse. The original chondrite was so fine-grained and fractured, and the variance in its grain-size distribution so large, that effects related to grain-size were relegated to the <63- m fraction. Impacts into ALH 85017 produced abundant, fine-grained debris, but otherwise the slopes of its size distributions were comparable to those from other experiments involving natural and fabricated terrestrial targets. The characteristic slopes of the chondrite's size distributions, however, were notably more constant over the entire nine-impact series than those from any of the terrestrial targets, a testament to the control over comminution apparently exerted by pre-existing fractures and other, microscopic damage in the meteorite. The enhancement in the finer fraction of debris from ALH 85017 indicates that ordinary chondrites in solar orbit would be very efficient contributors to the cosmic-dust complex. At the same time, the greater resistance to disruption displayed by ordinary chondrites relative to that exhibited by igneous rocks indicates that a selection effect could be operative between the annealed, ordinary-chondritic breccias and relatively weaker, differentiated meteorites. Preferential survival from their time in the regoliths of their parent bodies through their transit to Earth and passage through the atmosphere suggests that meteorite collections could be biased in favor of the ordinary chondrites.

Cintala, Mark J.↗

HDX–MS finds that partial unfolding with sequential domain activation controls condensation of a cellular stress marker

Eukaryotic cells form condensates to sense and adapt to their environment [S. F. Banani, H. O. Lee, A. A. Hyman, M. K. Rosen,Nat. Rev. Mol. Cell Biol.18, 285–298 (2017), H. Yoo, C. Triandafillou, D. A. Drummond,J. Biol. Chem.294, 7151–7159 (2019)]. Poly(A)-binding protein (Pab1), a canonical stress granule marker, condenses upon heat shock or starvation, promoting adaptation [J. A. Ribacket al.,Cell168, 1028–1040.e19 (2017)]. The molecular basis of condensation has remained elusive due to a dearth of techniques to probe structure directly in condensates. We apply hydrogen–deuterium exchange/mass spectrometry to investigate the mechanism of Pab1’s condensation. Pab1’s four RNA recognition motifs (RRMs) undergo different levels of partial unfolding upon condensation, and the changes are similar for thermal and pH stresses. Although structural heterogeneity is observed, the ability of MS to describe populations allows us to identify which regions contribute to the condensate’s interaction network. Our data yield a picture of Pab1’s stress-triggered condensation, which we term sequential activation (Fig. 1A), wherein each RRM becomes activated at a temperature where it partially unfolds and associates with other likewise activated RRMs to form the condensate. Subsequent association is dictated more by the underlying free energy surface than specific interactions, an effect we refer to as thermodynamic specificity. Our study represents an advance for elucidating the interactions that drive condensation. Furthermore, our findings demonstrate how condensation can use thermodynamic specificity to perform an acute response to multiple stresses, a potentially general mechanism for stress-responsive proteins.

Science & Technology - Other Topics↗

INTERACTION BETWEEN TWO CORONAL MASS EJECTIONS IN THE 2013 MAY 22 LARGE SOLAR ENERGETIC PARTICLE EVENT

We investigate the eruption and interaction of two coronal mass ejections (CMEs) during the large 2013 May 22 solar energetic particle event using multiple spacecraft observations. Two CMEs, having similar propagation directions, were found to erupt from two nearby active regions (ARs), AR11748 and AR11745, at ∼08:48 UT and ∼13:25 UT, respectively. The second CME was faster than the first CME. Using the graduated cylindrical shell model, we reconstructed the propagation of these two CMEs and found that the leading edge of the second CME caught up with the trailing edge of the first CME at a height of ∼6 solar radii. After about two hours, the leading edges of the two CMEs merged at a height of ∼20 solar radii. Type II solar radio bursts showed strong enhancement during this two hour period. Using the velocity dispersion method, we obtained the solar particle release (SPR) time and the path length for energetic electrons. Further assuming that energetic protons propagated along the same interplanetary magnetic field, we also obtained the SPR time for energetic protons, which were close to that of electrons. These release times agreed with the time when the second CME caught up with the trailing edge of the first CME, indicating that the CME-CME interaction (and shock-CME interaction) plays an important role in the process of particle acceleration in this event.

79 ASTRONOMY AND ASTROPHYSICS↗

Nonselective block by La3+ of Arabidopsis ion channels involved in signal transduction

Lanthanide ions such as La3+ are frequently used as blockers to test the involvement of calcium channels in plant and animal signal transduction pathways. For example, the large rise in cytoplasmic Ca2+ concentration triggered by cold shock in Arabidopsis seedlings is effectively blocked by 10 mM La3+ and we show here that the simultaneous large membrane depolarization is similarly blocked. However, a pharmacological tool is only as useful as it is selective and the specificity of La3+ for calcium channels was brought into question by our finding that it also blocked a blue light (BL)-induced depolarization that results from anion channel activation and believed not to involve calcium channels. This unexpected inhibitory effect of La3+ on the BL-induced depolarization is explained by our finding that 10 mM La3+ directly and completely blocked the BL-activated anion channel when applied to excised patches. We have investigated the ability of La3+ to block noncalcium channels in Arabidopsis. In addition to the BL-activated anion channel, 10 mM La3+ blocked a cation channel and a stretch-activated channel in patches of plasma membrane excised from hypocotyl cells. In root cells, 10 mM La3+ inhibited the activity of an outward-rectifying potassium channel at the whole cell and single-channel level by 47% and 58%, respectively. We conclude that La3+ is a nonspecific blocker of multiple ionic conductances in Arabidopsis and may disrupt signal transduction processes independently of any effect on Ca2+ channels.

Non-NASA Center↗

Probing the Wind Component of Radio Emission in Luminous High-redshift Quasars

We discuss a probe of the contribution of wind-related shocks to the radio emission in otherwise radio-quiet quasars. Given (1) the nonlinear correlation between UV and X-ray luminosity in quasars, (2) that such a correlation leads to higher likelihood of radiation-line-driven winds in more luminous quasars, and (3) that luminous quasars are more abundant at high redshift, deep radio observations of high-redshift quasars are needed to probe potential contributions from accretion disk winds. We target a sample of 50 z ≃ 1.65 color-selected quasars that span the range of expected accretion disk wind properties as traced by broad C iv emission. 3 GHz observations with the Very Large Array to an rms of ≈10 μJy beam -1 probe to star formation rates of ~400 M ⊙ yr -1 , leading to 22 detections. Supplementing these pointed observations are survey data of 388 sources from the LOFAR Two-meter Sky Survey Data Release 1 that reach comparable depth (for a typical radio spectral index), where 123 sources are detected. These combined observations reveal a radio detection fraction that is a nonlinear function of C iv emission-line properties and suggest that the data may require multiple origins of radio emission in radio-quiet quasars. We find evidence for radio emission from weak jets or coronae in radio-quiet quasars with low Eddington ratios, with either (or both) star formation and accretion disk winds playing an important role in optically luminous quasars and correlated with increasing Eddington ratio. Additional pointed radio observations are needed to fully establish the nature of radio emission in radio-quiet quasars.

79 ASTRONOMY AND ASTROPHYSICS↗

Artificial Neural Network Representation of Dynamic Criticality Excursion Experiment Data

This paper outlines time dependent considerations in applying the artificial neural network (ANN) paradigm to dynamic nuclear criticality excursion experiment data. The ANN amongst a variety of uses, can apply to function fitting of multiple input-multiple output data sets, pattern recognition, cluster analysis, and time series prediction. The ANN is a component of Machine Learning (ML) data analysis. The practical application of ML to criticality safety has been funded through the Y-12 Plant Directed Research and Demonstration (PDRD) program to support criticality safety and emergency planning efforts. The initial effort considered function fitting of criticality excursion data. Recent efforts successfully extended the ANN to criticality safety code validation. The initial ANN work applied to criticality excursions centered around multivariate function fitting of static (tabular) data taken from the French Consequences Radiologiques d'un Accident de Criticite (CRAC) and SILENE reactor campaigns. The former report (LeCorche) summarized important tabular data for the first 44 CRAC experiments. The initial ANN was trained to specific time-independent inputs, then applied to predict peak fission rate and total fissions for a wide variety of fissile solutions process conditions. This work extends that effort to apply the ANN paradigm to dynamic prediction of time-dependent criticality excursion experiment data. A dynamic criticality excursion in fissile solution represents a complex multiphysics phenomenon. Factors include among other things, coupled neutronic, multiphase fluid flow, temperature, pressure, acoustic shock, boiling heat transfer, reactivity feedback, and radiolytic gas considerations. There have been many efforts to model (over time) as coupled ordinary and (space) as partial differential equations (ODE, PDE). The ANN representation noted here does not rely on solving explicit ODE or PDEs. Rather the intent is to demonstrate that as a time series, the criticality excursion can be predicted by training specific ANN architectures to experimental input and output time series exclusively. The rationale for extending this work to the time-dependent regime is important in developing a 'criticality excursion emulator' solely on the basis of learning characteristics from experiment dynamic data. An emulator is different than a computer code simulator driven by the solution of ODEs and PDEs given select 'tuning parameters' that match outputs to experiments. A criticality excursion emulator, derived exclusively from an ANN or ML, could predict most likely excursion characteristics, trained from historic dynamic experiment data, presented with input values within the range of those experiments. The work noted shows that generic time-series ANN architectures can be satisfactorily applied to the prediction of dynamic, extremely chaotic criticality excursion power given an initial time dependent input series. In reality, an excursion emulator could be driven by user-supplied initial (t=0), time independent or constant values to generate time dependent outputs. Thus ongoing work includes developing a custom ANN such that initial dynamic conditions would be predicted. Those results then serve as inputs to a customized ANN architecture currently in development. (author)

42 ENGINEERING↗

Anomalous and Ungrouped Carbonaceous Chondrites in the US Antarctic Meteorite Collection and their Potential Relevance to Ryugu and Bennu

With two different carbonaceous asteroid sample return missions in full swing, attention has focused on what connections can be made between the asteroid samples and the wide range of carbonaceous chondrite meteorites in worldwide collections. The US Antarctic meteorite collection contains nearly 1000 carbonaceous chondrites of various types including many in well-established groups as well as ungrouped, unusual or anomalous groups [1]. Some of the latter have been included in, or are possibly related to, recently proposed new carbonaceous chondrite classifications – CA and CY chondrites [2,3]. In addition to these, there are numerous ungrouped samples that have properties intermediate between established groups (like CM and CO; [4]), distinct from any other groups [5], or have anomalous properties that might be attributable to parent body processes such as heating, fluid interaction, or impacts [6]. Some CM anomalous or ungrouped chondrites share spectral features with Ryugu, which has a small hydration peak arguably due to hydrated minerals left after either impact heating or shock in carbonaceous chondrites [7]. PCA 91008, PCA 02012, GRO 95566, and LEW 85311 are all CMs that have experienced heating or metamorphism that may be due to impacts, solar radiation, or radiogenic decay [6]. These samples all have low H contents, C/H (bulk), and low 17O [6,8] and may hold clues to understanding the mineralogy of Ryugu, or interpretations of its spectral properties. WIS 91600, on the other hand, appears to be related to several other highly altered CM [6], and shares properties with the newly proposed CY chondrites [6]. An understanding of this grouplet will also aid in the interpretation of Bennu samples which have strong hydration features. In addition, ungrouped carbonaceous chondrites may provide valuable insights into the aqueous alteration potentially recorded in Bennu and Ryugu samples; such as the relatively moderate aqueous alteration recognized and dated at 4-5 myr after CAIs in MAC 88107 (C2-ungrouped) by [9] to the extensive aqueous alteration apparent in MIL 090292 (C1-ungrouped) [10]. Finally, LON 94101/94102 is a brecciated CM chondrite with numerous lithologies. Its appearance is similar to some of the brecciated lithologies visible at the surface of Bennu and Ryugu [11]. Although CM chondrites with multiple lithologies are common (e.g., [12]), the lithologies are often difficult to resolve at the hand specimen scale and only after some detailed e-beam characterization are the subtle lithologic differences evident (e.g., [13]). LON 94102 contains visibly distinct clasts at the hand specimen scale, relatively rare for CM breccias. The largest CM chondrites by mass from the U.S. Antarctic meteorite collection may provide insights into the extent of brecciation and heterogeneity within more typical CM chondrite-like source asteroids. For example, recent curation CT scans [14] show possible clasts within in a subsplit of ALH 83100 (CM1/2) which is the largest CM chondrite or CM chondrite pairing group in the U.S. Antarctic collection with an original mass of 3.019 kg. Similar work on additional subsplits or meteorites may aid our understanding of heterogeneity within CM chondrites from Antarctica. Initial classification of CM chondrites in U.S. Antarctic meteorite collection includes preliminary pairing when petrographically similar CM chondrites that have been previously found in the same field area. The two largest CM chondrite preliminary pairing groups by mass are the ALH 83102 (CM2) pairing group with an original mass of 2.554 kg and the EET 96005 (CM2) pairing group with an original mass of 1.125 kg. However, preliminary pairing groups assigned at classification—intrinsically— do not include stones that are petrographically distinct. Rigorous pairing group studies of CM chondrites from specific field sites are needed to investigate if there is unrecognized heterogeneity in CM chondrites from Antarctica that may represent common asteroid impactors (pre-atmospheric entry asteroids/meteoroids). Detailed pairing studies have the potential to recognize initial stones with multiple lithologies and investigate if there are stones that only sample one of those respective lithologies in the collection. These groups of heated CMs, extensively hydrated CMs, intermediate between CM and CO chondrites, and brecciated samples are all potentially relevant to Bennu and Ryugu samples, where heating, hydration, and brecciation have all come into play. These bodies might also be comprised of material intermediate to CM and CO chondrites, or at least distinct from the well-established CC groups. These small and unusual groups of carbonaceous chondrites may help to unlock new information about early solar system processes and aid in the understanding of the evolution of these carbonaceous asteroids.

meteorites↗

The Prediction of Broadband Shock-Associated Noise Including Propagation Effects

An acoustic analogy is developed based on the Euler equations for broadband shock- associated noise (BBSAN) that directly incorporates the vector Green's function of the linearized Euler equations and a steady Reynolds-Averaged Navier-Stokes solution (SRANS) as the mean flow. The vector Green's function allows the BBSAN propagation through the jet shear layer to be determined. The large-scale coherent turbulence is modeled by two-point second order velocity cross-correlations. Turbulent length and time scales are related to the turbulent kinetic energy and dissipation. An adjoint vector Green's function solver is implemented to determine the vector Green's function based on a locally parallel mean flow at streamwise locations of the SRANS solution. However, the developed acoustic analogy could easily be based on any adjoint vector Green's function solver, such as one that makes no assumptions about the mean flow. The newly developed acoustic analogy can be simplified to one that uses the Green's function associated with the Helmholtz equation, which is consistent with the formulation of Morris and Miller (AIAAJ 2010). A large number of predictions are generated using three different nozzles over a wide range of fully expanded Mach numbers and jet stagnation temperatures. These predictions are compared with experimental data from multiple jet noise labs. In addition, two models for the so-called 'fine-scale' mixing noise are included in the comparisons. Improved BBSAN predictions are obtained relative to other models that do not include the propagation effects, especially in the upstream direction of the jet.

Miller, Steven↗

Transcriptomic Analyses Elucidate Adaptive Differences of Closely Related Strains of Pseudomonas aeruginosa in Fuel

Pseudomonas aeruginosa can utilize hydrocarbons, but different strains have various degrees of adaptation despite their highly conserved genome. P. aeruginosa ATCC 33988 is highly adapted to hydrocarbons, while P. aeruginosa strain PAO1, a human pathogen, is less adapted and degrades jet fuel at a lower rate than does ATCC 33988. We investigated fuel-specific transcriptomic differences between these strains in order to ascertain the underlying mechanisms utilized by the adapted strain to proliferate in fuel. During growth in fuel, the genes related to alkane degradation, heat shock response, membrane proteins, efflux pumps, and several novel genes were upregulated in ATCC 33988. Overexpression of alk genes in PAO1 provided some improvement in growth, but it was not as robust as that of ATCC 33988, suggesting the role of other genes in adaptation. Expression of the function unknown gene PA5359 from ATCC 33988 in PAO1 increased the growth in fuel. Bioinformatic analysis revealed that PA5359 is a predicted lipoprotein with a conserved Yx(FWY)xxD motif, which is shared among bacterial adhesins. Overexpression of the putative resistance-nodulation-division (RND) efflux pump PA3521 to PA3523 increased the growth of the ATCC 33988 strain, suggesting a possible role in fuel tolerance. Interestingly, the PAO1 strain cannot utilize n -C 8 and n -C 10 . The expression of green fluorescent protein (GFP) under the control of alkB promoters confirmed that alk gene promoter polymorphism affects the expression of alk genes. Promoter fusion assays further confirmed that the regulation of alk genes was different in the two strains. Protein sequence analysis showed low amino acid differences for many of the upregulated genes, further supporting transcriptional control as the main mechanism for enhanced adaptation. Lastly, these results support that specific signal transduction, gene regulation, and coordination of multiple biological responses are required to improve the survival, growth, and metabolism of fuel in adapted strains. This study provides new insight into the mechanistic differences between strains and helpful information that may be applied in the improvement of bacterial strains for resistance to biotic and abiotic factors encountered during bioremediation and industrial biotechnological processes.

54 ENVIRONMENTAL SCIENCES↗

Control and prevention measures for legionellosis in hospitals: A cross-sectional survey in Italy

Highlights: • Legionellosis risk is high in Italian hospitals with many hospitalizations. • Legionella contamination in the water system was assessed in 97% of hospitals. • Risk assessment was performed in 73% of hospitals but only 53% had a dedicated team. • Measures to reduce legionellosis risk were adopted in 93.3% of hospitals. • Training courses on Legionnaires’ disease were planned in 36.5% of hospitals. Risk assessment, environmental monitoring, and the disinfection of water systems are the key elements in preventing legionellosis risk. The Italian Study Group of Hospital Hygiene of the Italian Society of Hygiene, Preventive Medicine, and Public Health and the Italian Multidisciplinary Society for the Prevention of Health Care-Associated Infections carried out a national cross-sectional survey to investigate the measures taken to prevent and control legionellosis in Italian hospitals. A multiple-choice questionnaire was developed, comprising 71 questions regarding hospital location, general characteristics, clinical and environmental surveillance, and control and preventive measures for legionellosis in 2015. Overall, 739 hospitals were enrolled from February to June 2017, and 178 anonymous questionnaires were correctly completed and evaluated (response rate: 24.1%). The survey was conducted using the SurveyMonkey® platform, and the data were analyzed using Stata 12 software. Of the participating hospitals, 63.2% reported at least one case of legionellosis, of which 28.2% were of proven nosocomial origin. The highest case numbers were reported in the Northern Italy, in hospitals with a pavilion structure or cooling towers, and in hospitals with higher numbers of beds, wards and operating theaters. Laboratory diagnosis was performed using urinary antigen testing alone (31.9%), both urinary antigen testing and single antibody titer (17.8%), or with seroconversion also added (21.5%). Culture-based or molecular investigations were performed in 28.8% and 22.1% of the clinical specimens, respectively. The water systems were routinely tested for Legionella in 97.4% of the hospitals, 62% of which detected a positive result (> 1000 cfu/L). Legionella pneumophila serogroup 2–15 was the most frequently isolated species (58.4%). The most common control measures were the disinfection of the water system (73.7%), mostly through thermal shock (37.4%) and chlorine dioxide (34.4%), and the replacement (69.7%) or cleaning (70.4%) of faucets and showerheads. A dedicated multidisciplinary team was present in 52.8% of the hospitals, and 73% of the hospitals performed risk assessment. Targeted training courses were organized in 36.5% of the hospitals, involving nurses (30.7%), physicians (28.8%), biologists (21.5%), technicians (26.4%), and cleaners (11%). Control and prevention measures for legionellosis are present in Italian hospitals, but some critical aspects should be improved. More appropriate risk assessment is necessary, especially in large facilities with a high number of hospitalizations. Moreover, more sensitive diagnostic tests should be used, and dedicated training courses should be implemented.

54 ENVIRONMENTAL SCIENCES↗

Hybrid-PIC modeling of laser-plasma interactions and hot electron generation in gold hohlraum walls

The walls of the hohlraum used in experiments at the national ignition facility are heated by laser beams with intensities ∼1015 W/cm2, a wavelength of ∼1/3 μm, and pulse lengths on the order of a ns, with collisional absorption believed to be the primary heating mechanism. X-rays generated by the hot ablated plasma at the gold walls are then used to implode a target in the hohlraum interior. In addition to the collisional absorption of laser energy at the walls, non-linear laser-plasma interactions (LPI), such as stimulated Raman scattering and two plasmon decay, are believed to generate a population of supra-thermal electrons which, if present in the hohlraum, can have a deleterious effect on target implosion. We describe results of hohlraum modeling using a hybrid particle-in-cell code. To enable this work, new particle-based algorithms for a multiple-ion magneto-hydrodynamic (MHD) treatment, and a particle-based ray-tracing model were developed. The use of such hybrid methods relaxes the requirement to resolve the laser wavelength, and allows for relatively large-scale hohlraum simulations with a reasonable number of cells. But the non-linear effects which are believed to be the cause of hot electron generation can only be captured by fully kinetic simulations with good resolution of the laser wavelength. For this reason, we employ a two-tiered approach to hohlraum modeling. Large-scale simulations of the collisional absorption process can be conducted using the fast quasi-neutral MHD algorithm with fluid particle species. From these simulations, we can observe the time evolution of the hohlraum walls and characterize the density and temperature profiles. From these results, we can transition to smaller-scale highly resolved simulations using traditional kinetic particle-in-cell methods, from which we can fully model all of the non-linear laser-plasma interactions, as well as assess the details of the electron distribution function. We find that vacuum hohlraums should be stable to both two plasmon decay and stimulated Raman scattering instabilities for intensities ≤1015 W/cm2. In gas-filled hohlraums, shocks may be induced in the blowoff gold plasma, which leads to more complex density and temperatures profiles. The resulting effect on LPI stability depends strongly on the details of the profile, and it is possible for the gas-filled hohlraum to become unstable to two plasmon decay at 1015 W/cm2 if the quarter-critical surface reaches temperatures exceeding 1 keV.

Thoma, C.↗

Tearing mode instability in a multiple current sheet system

The tearing mode and magnetic reconnection are studied for multiple current sheet systems by two-dimensional magnetohydrodynamic (MHD) simulations. Both the linear and nonlinear evolution of this process are anaylsed for laminar perturbations. The results illustrate the existence of a linear regime with a symmetric and antisymmetric mode and agree with previous analytic results (Otto and Birk, 1992). The nonlinear evolution shows a number of interesting new features and may explain some properties in corresponding studies of turbulent reconnection. For wavelengths larger than twice the current sheet separation the evolution of antisymmetric modes leads to an entire reconfiguration of the magnetic field and converts a major portion of the magnetic energy into kinetic energy. Antisymmetric modes with smaller wavelengths and symmetric modes are found to saturate. The influence of the value of the resistivity on the reconnection rate decreases in the nonlinear evolution, and the ratio of current sheet separation to wavelength seems to be of major importance. A comparion of the dynamics of periodic current sheets with the evolution of only two current sheets indicates that some of the results for the periodic system also apply to the evolution of only two interacting current sheets. The results are discussed with respect to observations of large-scale plasma and magnetic field reconfigurations in the magnetosheath and near the Earth's bow shock.

Yan, M.↗

Observation of kinetic mix enhancement in thin-shell OMEGA implosions

Recent separated reactant experiments for thin-shell (6 µ⁢m) shock-driven implosions on OMEGA have demonstrated significant mix from a buried deuterated layer of the shell into the hot spot. Time resolved D 3 He-p reaction history data demonstrate a (50 ± 20)⁢ ps shift earlier in peak nuclear emission for separated reactant experiments relative to control, in contrast to past experimental data for thicker, 20 µ⁢m shells with no laser burn through that show a 75 ps delay due to the time required for hydrodynamic instabilities to develop. This contrast suggests that the mix mechanism was not hydrodynamic. Ion kinetic simulations utilizing fall line analyses show much closer agreement with mix yield and temperature than diffusion models, predicting a D 3 He-p mix yield of 1.7 × 10 9 as compared to the experimental value of 9.3⁢ (±2.1) × 10 8 . This is three orders of magnitude closer than the fall line analysis from a hydrodynamic simulation with an inline diffusive mix model, which suggests minimal mix and D 3 He-p yields of 5×10 5 . This makes kinetic mechanisms the only feasible explanation for the mix seen, demonstrating impact of a non-standard mix mechanism. An analytical model of this kinetic mix mechanism suggests that it can remain significant in situations when the shell expands significantly to low densities, and diffusive models predict negligible mix. Finally, kinetic mix will impact multiple types of high energy density, laser-driven fusion experiments including high-adiabat direct drive cryoexperiments, nuclear cross section experiments, and thin-shell polar direct drive experiments used to tune heat conduction models.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Wind Tunnel Investigation of the Supersonic Stage Separation Aerodynamics of a Generic 0.0175-Scale Bimese Two-Stage-to-Orbit Reusable Launch Vehicle Configuration

A wind tunnel investigation was conducted of the supersonic stage separation aerodynamics of a generic two-stage-to-orbit bimese wingbody configuration in the NASA Langley Research Center Unitary Plan Wind Tunnel. Proximity and isolated model testing was conducted at Mach numbers of 2.3, 3.0, and 4.5 and a unit Reynolds number of 2.0 million per foot using 0.0175-scale models of the Langley Glide-Back Booster concept designated as the orbiter and booster in belly-to-belly and back-to-belly configurations. Longitudinal forces and moments were obtained on both models and surface static pressure measurements were obtained on the orbiter model at 328 relative proximity locations and at relative angles of attack of 0 degrees and 5 degrees. The test results supported a larger effort to develop and validate experimental and computational tools applicable to the design and simulation of stage separation and abort procedures for reusable launch vehicles composed of multiple bodies, including winged bodies. An initial proof-of-concept experiment featuring low-cost uninstrumented models was conducted to verify an emerging automated model control system and new support system hardware, and to identify potential model and support system blockage and unsteady aerodynamics/model dynamics prior to committing to higher-fidelity instrumented models. This investigation led to upgrades in the facility stage separation hardware, calibration and testing techniques and capabilities, and data analysis and documentation methodologies that have been extended to the more recent NASA Constellation and Space Launch System crew and cargo launch vehicle programs. A virtual diagnostics interface methodology was used to facilitate the design of the stage separation support hardware, to position the models in the test section, and to define the experimental test space. Advances in the facility automated model positioning system established a foundation for the development of a continuous-sweep data acquisition technique that is responsible for significant productivity improvements to the current NASA Space Launch System test program. The automated model positioning capability was leveraged to conduct a companion statistically-designed stage separation experiment requiring randomization of the relative proximity positions of the orbiter and booster models. The respective zones of influence and interference effects of the orbiter and booster were identified from three-dimensional scatter plots, contour and influence maps, and two-dimensional plotting methods. The highly-nonlinear, shock-dominated aerodynamic characteristics of the orbiter and booster in the Unitary Plan Wind Tunnel exhibited good agreement with independent test data obtained in a NASA Marshall Space Flight Center wind tunnel and with computational fluid dynamics predictions using a compressible, three-dimensional flow solver and an inviscid, unstructured Cartesian method.

Erickson, Gary E.↗