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At least 883 records · Page 49

Estrous Cyclicity of Mice During Simulated Weightlessness

Hindlimb unloading (HU) is a rodent model system used to simulate weightlessness experienced in space. However, some effects of this approach on rodent physiology are under-studied, specifically the effects on ovarian estrogen production which drives the estrous cycle. To resolve this deficiency, we conducted a ground-based validation study using the HU model, while monitoring estrous cycles in 16-weeks-old female C57BL6 mice. Animals were exposed to HU for 12 days following a 3 day HU cage acclimation period, and estrous cycling was analyzed in HU animals (n22), normally loaded HU Cage Pair-Fed controls (CPF; n22), and Vivarium controls fed ad libitum (VIV; n10). Pair feeding was used to control for potential nutritional deficits on ovarian function. Vaginal cells were sampled daily in all mice via saline lavage. Cells were dried and stained with crystal violet, and the smears evaluated using established vaginal cytology techniques by two individuals blinded to the animal treatment group. Estrous cyclicity was disrupted in nearly all HU and CPF mice, while those maintained in VIV had an average normal cycle length of 4.8 0.5 days, with all stages in the cycle visibly observed. CPF and HU animals arrested in the diestrous phase, which precedes the pre-ovulatory estrogen surge. Additionally, infection-like symptoms characterized by vaginal discharge and swelling arose in several HU animals, which we suspect was due to an inability of these mice to properly groom themselves, andor due to the change in the gravity vector relative to the vaginal opening, which prevented drainage of the lavage solution. Pair-feeding resulted in similar weight gains of HU and CPF (1.5 vs 3.0, respectively). The current results indicate that pair-feeding controlled weight gain and that the HU cage alone influenced estrous cyclicity. Thus, longer acclimation needs to be tested to determine if and when normal estrous cycling resumes in non-loaded mice in HU cages prior to HU testing. Future studies might also examine whether modifications to the vaginal lavage procedure might prevent the onset of the infection-like symptoms, and allow estrous cyclicity to be measured in this model system.

fertilit↗

Sensor Monitors Force On Filler Wire During Welding

Sensor measures lateral contact force between filler wire and weldment. Output of sensor fed as input to motorized wire-position controller, which strives to maintain desired contact force. Sensor built into wire-feed assembly. Pivoting arm transmits lateral force on tip of wire to load cell. Setscrews prevent underload and overload on load cell.

Morris, Tim↗

Applications of Advanced Nondestructive Evaluation (NDE) and Structural Health Monitoring (SHM) Techniques to Address Safety of Flight Issues on NASA Spacecraft

Advanced NDE and SHM techniques are critical for ensuring the reliability and safety of NASA launch vehicles and spacecraft. NDE techniques such as infrared thermography, THz imaging, x-ray computed tomography and backscatter x-ray are used to detect indications of damage in spacecraft components and structures. Additionally, sensor and measurement systems are integrated into spacecraft to provide SHM to detect damaging events that occur during flight such as debris impacts during launch and assent or from micrometeoroid and orbital debris, or excessive loading due to anomalous flight conditions. A number of examples will be provided of how these NDE and SHM techniques have been applied to resolve safety critical inspection concerns for the Space Shuttle, International Space Station (ISS), and a variety of launch vehicles and unmanned spacecraft.

Advanced Nondestructive Evaluation (NDE)↗

Phase transition kinetics revealed by in situ x-ray diffraction in laser-heated dynamic diamond anvil cells

We report successful coupling of dynamic loading in a diamond anvil cell and stable laser heating, which enables compression rates up to 500 GPa/s along high-temperature isotherms. Dynamic loading in a diamond-anvil cell allows exploration of a wider range of pathways in the pressure-temperature space compared to conventional dynamic compression techniques. By x-ray diffraction, we are able to characterize and monitor the structural transitions with the appropriate time resolution i.e., millisecond timescales. Using this method, we investigate the γ − ε phase transition of iron under dynamic compression, reaching compression rates of hundreds of GPa/s and temperatures of 2000 K. Our results demonstrate a distinct response of the γ − ε and α − ε transitions to the high compression rates achieved, possibly due to the different transition mechanisms. These findings open up new avenues to study tailored dynamic compression pathways in the pressure-temperature space and highlight the potential of this platform to capture kinetic effects (over ms time scales) in a diamond anvil cell. Published by the American Physical Society 2024

Ricks, Matthew (ORCID:000000015315612X)↗

Smart Inverters for Seamless Reconnection of Isolated Residential Microgrids to Utility Grid

This paper proposes an approach to achieve seamless reconnection of isolated residential microgrids to utility grid. Any abnormal condition on the grid side results in isolating the residential microgrid from utility grid, and giving the full responsibility of supplying household loads to local distributed generation (DG) units. However, after resolving the abnormal condition on the grid side, the residential microgrid needs to seamlessly reconnect to the main grid. To this end, a seamless transition algorithm is presented which monitors the system condition in real time, and coordinates the operation of all inverter-based DG units in residential microgrid before reconnection to the main grid. A modified control scheme is proposed for single-phase inverters which turns them into smart inverters enable to interact with seamless transition algorithm. The proposed approach synchronizes each phase voltage with its respective grid-side voltage in order to seamlessly reconnect the residential microgrid to the main grid. Case study results are carried out in PSCAD/EMTDC environment to verify the validity of proposed method.

Pilehvar, Mohsen S.↗

Assessing Nutrient and Carbon Responses to Agricultural Conservation Practices in Two Midwest Watersheds

The United States is undertaking efforts to transition to a low-carbon economy in response to the heightened impacts of climate change, which are largely attributed to decades of carbon-intensive development. A concerted effort by the energy sector to achieve net-zero carbon emissions is aimed at strategically reducing the carbon footprint of the energy supply chain, particularly in the production of feedstocks for biofuels. The production of biofuels relies heavily on land use and management practices in biomass cultivation. Among other factors, soil organic carbon (SOC) plays a crucial role in the biofuel carbon cycle, impacting land productivity, greenhouse gas emissions, and water quality. The process of carbon drawdown during plant growth and storage helps to mitigate the release of carbon dioxide (CO 2 ) into the atmosphere. Another key parameter is the release of nitrous oxide (N 2 O) — a potent greenhouse gas with a global warming potential 273 times that of CO 2 — from soil. While prioritizing low-carbon production, sustainable bioenergy also necessitates improved water quality and ecosystem services. Agricultural conservation practices can contribute to enhanced soil health and reduce nutrient and sediment loss into streams. However, studies that explore the broader impact of these practices on soil carbon storage and N 2 O emissions at a watershed scale are limited. Specifically, our understanding of how low-carbon feedstock production and management affect nutrient cycle dynamics is incomplete. This study assesses watershed responses to land management practices in two agriculturally dominant watersheds: the Raccoon River watershed and the Southfork of Iowa River watershed. The study focuses on carbon and nutrient dynamics, characterizing spatial and temporal variations in SOC, N 2 O, and nutrient loadings to examine the relationship between nutrient and carbon responses and agricultural conservation practices. The study employs the newly developed Soil Water Analysis Tool for Carbon (SWAT-C) model, calibrated using 20 years’ of climate and water monitoring data, to simulate and evaluate two agricultural conservation practices: no-till and crop residue harvest with cover crop planting. We compared the calibrated SWAT-C model with a historical baseline model, which allowed us to analyze various aspects of the watershed, including stream flow, suspended sediments, nitrogen, phosphorus, organic carbon, SOC at different depths, and N 2 O emissions from topsoil. Our SWAT-C modeling results indicate that, compared with results from the historical baseline model, no-till practices are positively correlated with SOC accumulation, reduced N 2 O emissions, and decreased soil loss. We also observed no change or slightly increased nitrogen and phosphorus loss to water bodies in the watersheds compared with the baseline. Conversely, crop residue harvest with cover crop planting improved water quality by reducing nutrient and soil losses but also increased N 2 O emissions. This SWAT-C modeling study establishes the groundwork for further smaller-scale and/or sub-basin-level assessments of nitrogen, phosphorus, and carbon cycles. It provides valuable science-based insights to inform policy decisions — particularly in the context of transitioning to biofuel feedstock production in these watersheds — by addressing concerns about nutrient exports to local water bodies and, ultimately, the Mississippi River.

54 ENVIRONMENTAL SCIENCES↗

Debris prevention analysis for DFI/OFI/OEI (STS-26 configuration only)

The first 3 shuttle flights to use the Redesigned Solid Rocket Motors (RSRMs) will utilize Development Flight Instrumentation (DFI), as well as Operational Flight Instrumentation (OFI), and Operational Environment Instrumentation (OEI). The OFI consists of high pressure transducers used on both RSRMs to monitor the igniter and motor chamber pressure. DFI consists of assorted strain gages, temperature sensors, accelerometers, girth gages, and low level pressure transducers. The latter are installed on the left hand booster to measure post-separation aerodynamic loading. OEI consistes of temperature sensors. After Flight 3, all DFI gages are to be deleted, and only OFI and OEI will be used for subsequent flights. This report deals specifically with debris prevention and hazards concernig the STS-26 flight DFI configuration only. Continued analysis is being done that will adequately address the debris hazards associated with the STS-27 and subsequent flight DFI configurations.

Richi, Glen A.↗

Microstructure-Sensitive Investigation of Fracture Using Acoustic Emission Coupled With Electron Microscopy

A novel technique using Scanning Electron Microscopy (SEM) in conjunction with Acoustic Emission (AE) monitoring is proposed to investigate microstructure-sensitive fatigue and fracture of metals. The coupling between quasi in situ microscopy with actual in situ nondestructive evaluation falls into the ICME framework and the idea of quantitative data-driven characterization of material behavior. To validate the use of AE monitoring inside the SEM chamber, Aluminum 2024-B sharp notch specimen were tested both inside and outside the microscope using a small scale mechanical testing device. Subsequently, the same type of specimen was tested inside the SEM chamber. Load data were correlated with both AE information and observations of microcracks around grain boundaries as well as secondary cracks, voids, and slip bands. The preliminary results are in excellent agreement with similar findings at the mesoscale. Extensions of the application of this novel technique are discussed.

Wisner, Brian↗

Estrous Cyclicity in Mice During Simulated Weightlessness

Hindlimb unloading (HU) is a rodent model system used to simulate weightlessness experienced in space. However, some effects of this approach on rodent physiology are under-studied, specifically the effects on ovarian estrogen production which drives the estrous cycle. To resolve this deficiency, we conducted a ground-based validation study using the HU model, while monitoring estrous cycles in 16-weeks-old female C57BL6 mice. Animals were exposed to HU for 12 days following a 3 day HU cage acclimation period, and estrous cycling was analyzed in HU animals (n=22), normally loaded HU Cage Pair-Fed controls (CPF; n=22), and Vivarium controls fed ad libitum (VIV; n=10). Pair feeding was used to control for potential nutritional deficits on ovarian function. Vaginal cells were sampled daily in all mice via saline lavage. Cells were dried and stained with crystal violet, and the smears evaluated using established vaginal cytology techniques by two individuals blinded to the animal treatment group. Estrous cyclicity was disrupted in nearly all HU and CPF mice, while those maintained in VIV had an average normal cycle length of 4.8+/- 0.5 days, with all stages in the cycle visibly observed. CPF and HU animals arrested in the diestrous phase, which precedes the pre-ovulatory estrogen surge. Additionally, infection-like symptoms characterized by vaginal discharge and swelling arose in several HU animals, which we suspect was due to an inability of these mice to properly groom themselves, and/or due to the change in the gravity vector relative to the vaginal opening, which prevented drainage of the lavage solution. Pair-feeding resulted in similar weight gains of HU and CPF (1.5% vs 3.0%, respectively). The current results indicate that pair-feeding controlled weight gain and that the HU cage alone influenced estrous cyclicity. Thus, longer acclimation needs to be tested to determine if and when normal estrous cycling resumes in non-loaded mice in HU cages prior to HU testing. Future studies might also examine whether modifications to the vaginal lavage procedure might prevent the onset of the infection-like symptoms, and allow estrous cyclicity to be measured in this model system. Research supported by NNX15AB48G to JST.

Hindlimb Unloading↗

Hydro-isostatic deflection and tectonic tilting in the central Andes: Initial results of a GPS survey of Lake Minchin shorelines

Sufficiently large lake loads provide a means of probing rheological stratification of the crust and upper mantle. Lake Minchin was the largest of the late Pleistocene pluvial lakes in the central Andes. Prominent shorelines, which formed during temporary still-stands in the climatically driven lake level history, preserve records of lateral variations in subsequent net vertical motions. At its maximum extent the lake was 140 m deep and spanned 400 km N-S and 200 km E-R. The load of surficial water contained in Lake Minchin was sufficient to depress the crust and underlying mantle by 20-40 m, depending on the subjacent rheology. Any other differential vertical motions will also be recorded as departures from horizontality of the shorelines. We recently conducted a survey of shoreline elevations of Lake Minchin with the express intent of monitoring the hydro-isostatic deflection and tectonic tilting. Using real-time differential Global Positioning System (GPS), we measured topographic profiles across suites of shorelines at 15 widely separated locations throughout the basin. Horizontal and vertical accuracies attained are roughly 30 and 70 cm, respectively. Geomorphic evidence suggests that the highest shoreline was occupied only briefly (probably less than 200 years) and radiocarbon dates on gastropod shells found in association with the shore deposits constrain the age to roughly 17 kyr. The basin-side pattern of elevations of the highest shoreline is composed of two distinct signals: (27 +/- 1) m of hydro-isostatic deflection due to the lake load, and a planar tilt with east and north components of (6.8 +/- 0.4) 10(exp -5) and 9-5.3 +/- 0.3) 10(exp -5). This rate of tilting is too high to be plausibly attributed to steady tectonism, and presumably reflects some unresolved combination of tectonism plus the effects of oceanic and lacustrine loads on a laterally heterogeneous substrate. The history of lake level fluctuations is still inadequately known to allow detailed inferences of crust and mantle rheology. However, it is already clear that the effective elastic plate thickness is closer to 40 km than the 60-70 km crustal thickness in the central Andes and the effective viscosity is less than 5 10(exp 20) Pa s.

Bills, Bruce G.↗

Modular Composite Damage Detection System (MCDDS)

The 2020 CIF funded Modular Composite Damage Detection System (MCDDS) aims to advance the technology of damage detection in composites in the areas of thin, flexible damage detection panel construction, encapsulation techniques in composite matrixes, innovative integration and interfacing techniques with flexible embedded sensory panels, repair methods of composite materials, and improved packaging of the MCDDS. Custom thin, flexible polyimide printed circuit boards (PCBs) were designed and procured for fabrication of test specimens. A custom interface PCB was designed and fabricated to monitor the newly developed damage detection test specimens. Test specimens were fabricated using the novel flexible circuitry that were either fully or partially encapsulated. The fully encapsulated specimens were used to demonstrate embedded damage detection concepts and the partially encapsulated specimens were used to demonstrate surface-mounted (laminate) damage detection concepts. The new composite damage detection test specimens were tested and evaluated to determine if the novel damage detection sensory panels could withstand low-cycle threshold fatigue loading per a modified version of American Society for Testing and Materials (ASTM) D 7264 for up to 50 cycles without void formation. The data for multiple sensory panels indicates that the newly fabricated sensory panels are robust and do indeed withstand three-point bend flexural loading for more than 50 cycles. Additionally, testing was performed to determine if damage could be detected in damage detection test specimens subjected to failure using a 3-point bend test per a modified version of ASTM D 7264. Again, multiple sensory panels (both fully and partially encapsulated) showed the ability to detect damage to specimens subjected to failure per a modified version of ASTM D 7264. Additionally, a novel concept for tracking and monitoring of cracking (and potentially delamination) in composites was designed and developed that utilizes painted-on conductors.

Christopher Forney↗

Additive Manufacturing of Self‐Sensing Carbon Fiber Composites

Carbon fiber-reinforced polymer (CFRP) composites have gained substantial attention across various industries owing to their exceptional mechanical properties and lightweight nature. The emergence of additive manufacturing technologies brings new opportunities to the industry, offering advantages such as design freedom, rapid prototyping, and customization. However, the fabrication of CFRP composites through 3D printing techniques poses challenges pertaining to low resolution and limitations in complex geometry realization. This work introduces digital light processing printing as a versatile, high-resolution method ideal for CFRP composite fabrication. Furthermore, the development and characterization of CFRP are focused on and the manipulation of mechanical properties through variations in matrix resins and fiber loadings is investigated, showcasing the versatility of CFRP composites for tailored applications. Additionally, the integration of self-sensing capabilities in CFRP structures is explored, which opens up opportunities for applications in smart components for automotive and structural health monitoring.

carbon fiber composites↗

Using real-time data analysis to conduct next-generation synchrotron fatigue studies

Next-generation experimental techniques, like high energy X-ray diffraction microscopy (HEDM), usher in new opportunities to collect the grain-scale data necessary for understanding the evolving processes that drive fatigue failure. In this study, we present a framework for monitoring the evolution of a deforming polycrystal, in real-time, by applying principal component analysis (PCA) to raw X-ray diffraction image data. We applied this framework to inform in-situ HEDM measurements of a cyclically loaded Inconel-718 superalloy. Further, we discovered correlations between PCA of the diffraction data and the physical processes in the polycrystal. Lastly, we discuss extending this framework in future HEDM fatigue studies.

36 MATERIALS SCIENCE↗

A Half-Bridge On-State Voltage Sensor for In-Situ Measurements

Current prognostics for life expectancy of power semiconductors utilize off-line measurements of the on-state resistance. Presented is a half-bridge (HB) on-state voltage (VON) sensor capable of being utilized for in-situ measurements enabling continuous and real-time monitoring of the on-state resistance. The design provides a single VON measurement output that contains independent VON information of both high and low side devices in a HB leg. The measurement output is referenced from the middle point of the HB and combines the two complementary VON sensing outputs. This system reduces the number of components, required number of analog signal processing circuits, and ADC channels for in-situ health monitoring of a power electronics system. These reductions will facilitate integration of the VON sensor with the gate driver board and achieve low system profile. The experimental results demonstrate the proposed VON measurements while switching with inductive and resistive loads.

Roy, Chondon↗

Fracture control plan for propellant and pressurant tanks

To assure the functional integrity of the GLL-RPM fuel and pressurized gas tank, all activities during entire lifetime of the tank, from manufacture to the end of the mission, must be monitored. Specifications are given for all procedures which influence the tank structure including design, manufacture, cleaning, test operations, and storage. Tests to be conducted to demonstrate that requirements of the fracture control plan are satisfied include static tests, cyclic load tests, proof tests, He-leak test, and acceptance tests.

Source record↗

Likelihood alarm displays

In a likelihood alarm display (LAD) information about event likelihood is computed by an automated monitoring system and encoded into an alerting signal for the human operator. Operator performance within a dual-task paradigm was evaluated with two LADs: a color-coded visual alarm and a linguistically coded synthetic speech alarm. The operator's primary task was one of tracking; the secondary task was to monitor a four-element numerical display and determine whether the data arose from a 'signal' or 'no-signal' condition. A simulated 'intelligent' monitoring system alerted the operator to the likelihood of a signal. The results indicated that (1) automated monitoring systems can improve performance on primary and secondary tasks; (2) LADs can improve the allocation of attention among tasks and provide information integrated into operator decisions; and (3) LADs do not necessarily add to the operator's attentional load.

Sorkin, Robert D.↗

Future space transportation system architecture avionics requirements

NASA began a multi-center study in January 1993 to examine options for providing the most cost effective space transportation system in the future. The key advanced avionics requirements for these vehicle concepts are envisioned to provide significantly improved operational efficiency and effectiveness. It is very desirable to have adaptive guidance, navigation, and control approaches that will allow launch and return in almost any weather condition. The vehicles must be able to accommodate atmospheric density variations and winds without software changes. The flight operations must become much more autonomous in all flight regimes like an aircraft, and preflight checkout should make use of the onboard systems. When the vehicle returns to the launch site, subsystem health must be known and maintenance tasks scheduled accordingly. Ground testing of most subsystems must be eliminated. Also, the health monitoring system must be designed to enhance the ability to abort the mission significantly and save the crew and the vehicle. The displays and controls must be much less complex than current systems and must significantly reduce pilot work load. It is important to have low power, light weight displays and controls. Rendezvous and docking and all flight phases must have autopilot capability to reduce pilot work load for routine operations and in abort situations. The vehicles must have the demonstrated ability to return to the launch site. Abort from all mission phases can put additional demands on the communications system.

Stone, Howard↗

Structural Analysis Methods for Structural Health Management of Future Aerospace Vehicles

Two finite element based computational methods, Smoothing Element Analysis (SEA) and the inverse Finite Element Method (iFEM), are reviewed, and examples of their use for structural health monitoring are discussed. Due to their versatility, robustness, and computational efficiency, the methods are well suited for real-time structural health monitoring of future space vehicles, large space structures, and habitats. The methods may be effectively employed to enable real-time processing of sensing information, specifically for identifying three-dimensional deformed structural shapes as well as the internal loads. In addition, they may be used in conjunction with evolutionary algorithms to design optimally distributed sensors. These computational tools have demonstrated substantial promise for utilization in future Structural Health Management (SHM) systems.

Tessler, Alexander↗