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At least 883 records · Page 49

Mammalian Chromosome Analysis and Sorting by Flow Cytometry

The analysis of chromosomes by flow cytometry is termed flow cytogenetics, and it involves the analysis and sorting of single mitotic chromosomes in suspension. The study of flow karyograms provides insight into chromosome number and structure to provide information on chromosomal DNA content and can enable the detection of deletions, translocations, or any forms of aneuploidy. Beyond its clinical applications, flow cytogenetics greatly contributed to the Human Genome Project through the ability to sort pure populations of chromosomes for gene mapping, cloning, and the construction of DNA libraries. Maximizing the potential of these important applications of flow cytogenetics relies on precise instrument setup and optimal sample processing, both of which impact the accuracy and quality of the data that are generated. This article is a compilation of the existing protocols that describe the stepwise methodology of accumulating, isolating, and staining metaphase chromosomes to prepare single-chromosome suspensions for flow cytometric analysis and sorting. Although the chromosome preparation protocols have remained largely unchanged, cytometer technology has advanced dramatically since these protocols were originally developed. Advances in cytometry technologies offer new and exciting approaches for understanding and monitoring chromosomal aberrations, but the hallmark of these protocols remains their simplicity in methodologies and reagent requirements and the accuracy of data resolvable to every chromosome of the cell.

59 BASIC BIOLOGICAL SCIENCES↗

Numerical prediction of aeroacoustic jet-flap flows

Turbulent boundary layer flows departing an aerodynamic surface are experimentally verified to be strong sources of noise. Analysis of the source tensor of the Lighthill equation identifies important gradients of mean and fluctuating velocity components. Transition distributions of mean flow and select fluctuating velocity correlations, within the region immediately downstream of a sharp edged flap terminus are established by finite element solution of a parabolized form of the time-averaged steady flow Navier-Stokes equations closed with a turbulence kinetic energy model. Numerical solutions are presented which quantize the localized large mean flow accelerations and resultant peaks in the distribution of turbulence kinetic energy in the near wake region. Results are verified by comparison to experiment.

Baker, A. J.↗

Bubble Generation in a Continuous Liquid Flow Under Reduced Gravity Conditions

The present work reports a study of bubble generation under reduced gravity conditions for both co-flow and cross-flow configurations. Experiments were performed aboard the DC-9 Reduced Gravity Aircraft at NASA Glenn Research Center, using an air-water system. Three different flow tube diameters were used: 1.27, 1.9, and 2.54 cm. Two different ratios of air injection nozzle to tube diameters were considered: 0.1 and 0.2. Gas and liquid volumetric flow rates were varied from 10 to 200 ml/s. It was experimentally observed that with increasing superficial liquid velocity, the bubbles generated decreased in size. The bubble diameter was shown to increase with increasing air injection nozzle diameters. As the tube diameter was increased, the size of the detached bubbles increased. Likewise, as the superficial liquid velocity was increased, the frequency of bubble formation increased and thus the time to detach forming bubbles decreased. Independent of the flow configuration (for either single nozzle or multiple nozzle gas injection), void fraction and hence flow regime transition can be controlled in a somewhat precise manner by solely varying the gas and liquid volumetric flow rates. On the other hand, it is observed that uniformity of bubble size can be controlled more accurately by using single nozzle gas injection than by using multiple port injection, since this latter system gives rise to unpredictable coalescence of adjacent bubbles. A theoretical model, based on an overall force balance, is employed to study single bubble generation in the dynamic and bubbly flow regime. Under conditions of reduced gravity, the gas momentum flux enhances bubble detachment; however, the surface tension forces at the nozzle tip inhibits bubble detachment. Liquid drag and inertia can act either as attaching or detaching force, depending on the relative velocity of the bubble with respect to the surrounding liquid. Predictions of the theoretical model compare well with performed experiments. However, at higher superficial,liquid velocities, the bubble neck length begins to significantly deviate from the value of the air injection nozzle diameter and thus the theory no longer predicts the experiment behavior. Effects of fluid properties, injection geometry and flow conditions on generated bubble size are investigated using the theoretical model. It is shown that bubble diameter is larger in a reduced gravity environment than in a normal gravity environment at similar flow condition and flow geometry.

Pais, Salvatore Cezar↗

Low-dimensional description of the dynamics in separated flow past thick airfoils

Results are presented for the numerical simulation of unsteady viscous incompressible flow past thick airfoils. Specifically, flow past a NACA 4424 at an angle of attack of 2.5 deg and Reynolds numbers in the range of 1700-4000 has been simulated using the spectral element method. At these conditions the flow is separatedd and an unsteady wake is formed. Application of the method of empirical eigenfunction reveals the structure of the most energetic components of the flow. These are found to occur in pairs that, through phase exchange, are responsible for the vortex shedding. A set of ordinary differential equations is obtained for the amplitudes of these eigenfunctions by a Galerkin projection of the Navier-Stokes equations. The solutions of the model system are compared with the full simulation. The work is of relevance to the transition process and observed routes to chaos in airfoil wakes.

Deane, Anil E.↗

On the Kernel function of the integral equation relating the lift and downwash distributions of oscillating finite wings in subsonic flow

This report treats the Kernel function of an integral equation that relates a known prescribed downwash distribution to an unknown lift distribution for a harmonically oscillating finite wing in compressible subsonic flow. The Kernel function is reduced to a form that can be accurately evaluated by separating the Kernel function into two parts: a part in which the singularities are isolated and analytically expressed and a nonsingular part which may be tabulated. The form of the Kernel function for the sonic case (Mach number 1) is treated separately. In addition, results for the special cases of Mach number of 0 (incompressible case) and frequency of 0 (steady case) are given. The derivation of the integral equation which involves this Kernel function is reproduced as an appendix. Another appendix gives the reduction of the form of the Kernel function obtained herein for the three-dimensional case to a known result of Possio for two-dimensional flow. A third appendix contains some remarks on the evaluation of the Kernel function, and a fourth appendix presents an alternate form of expression for the Kernel function.

Watkins, Charles E↗

Separated flows near the nose of a body of revolution

The solution of the Navier-Stokes equations for the problem of cross-flow separataion about a deforming cylinder was achieved by iteration. It was shown that the separation starts at the rear stagnation point and the point of primary separation moves upstram along the cylinder surface. A general method of linear stability analysis for nonparallel external flows was constructed, which consists of representing the eigenfunctions with complete orthogonal sets and forms characteristic equations with the Galerkin method. The method was applied to the Kovasznay flow which is an exact solution of the Navier-Stokes equation. The results show that when the critical parameter is exceeded, there are only a few isolated unstable eigen-frequencies. Another exact solution is shown to be absolutely and monotonically stable with respect to infinitesimal disturbances of all frequencies. The flow is also globally, asymptotically, and monotonically stable in the mean with respect o three-dimensional disturbances. This result forms the sound foundation of rigorous stability analysis for nonparallel flows, and provides an invaluable test ground for future studies of nonparallel flows in which the basic states do not posses exact solutions. The application of this method to the study of the formation of spiral vorticies near the nose of a rotating body of revolution is underway. The same method will be applied to the stability analysis of reversed flow over a plate with suction.

Lin, S. P.↗

Pickup Ion Distributions from Three Dimensional Neutral Exospheres

Pickup ions formed from ionized neutral exospheres in flowing plasmas have phase space distributions that reflect their source's spatial distributions. Phase space distributions of the ions are derived from the Vlasov equation with a delta function source using three.dimensional neutral exospheres. The ExB drift produced by plasma motion picks up the ions while the effects of magnetic field draping, mass loading, wave particle scattering, and Coulomb collisions near a planetary body are ignored. Previously, one.dimensional exospheres were treated, resulting in closed form pickup ion distributions that explicitly depend on the ratio rg/H, where rg is the ion gyroradius and H is the neutral scale height at the exobase. In general, the pickup ion distributions, based on three.dimensional neutral exospheres, cannot be written in closed form, but can be computed numerically. They continue to reflect their source's spatial distributions in an implicit way. These ion distributions and their moments are applied to several bodies, including He(+) and Na(+) at the Moon, H(+2) and CH(+4) at Titan, and H+ at Venus. The best places to use these distributions are upstream of the Moon's surface, the ionopause of Titan, and the bow shock of Venus.

Hartle, R. E.↗

Dominant Controls on Preferential Flow and Their Implications for Future Soil Water Fluxes

Abstract Soil water flow, particularly preferential flow (PF), is a critical control on hydrological and biogeochemical processes, including groundwater recharge, contaminant transport, and carbon cycling. However, it remains challenging to predict PF occurrence across large environmental gradients. Here, we developed a deep learning (DL) model to estimate event‐scale soil water flow velocity and the probability of PF occurrence using high‐frequency soil moisture and precipitation data from 33 sites across the National Ecological Observatory Network. The model demonstrated high skill in predicting the binary occurrence of PF (91% F1‐score; 85% accuracy) but the performance was limited in predicting soil water velocity ( R 2 = 0.31). We found that precipitation characteristics (duration, volume, and intensity) were the most important predictors for soil water velocity. Among the non‐precipitation event variables, sand content showed relatively high predictive skill, though differences among non‐event climate variables were generally modest. Lower sand content was associated with increased predicted soil water velocity, a finding that highlights the role of soil structure in producing more non‐uniform flow, which contrasts with traditional uniform flow models. Projecting a reduced DL model under both moderate and high‐emissions future climate scenarios (2060–2099 Representative Concentration Pathways 4.5 and 8.5), we found ∼7.3% increase under RCP4.5 and ∼15% under RCP8.5 of soil water velocities compared to the historical simulation, while modeled likelihood of PF changed little. These findings suggest climate change is not making PF more frequent, but it is making existing PF pathways more efficient with important consequences for associated nutrient and contaminant transport under climate change. Plain Language Summary Water movement in soil is critical for water quality. While often modeled as a uniform flow process, in reality water moves rapidly through cracks and burrows in what is called “preferential flow” (PF), which limits natural filtration and can transport pollutants. We developed a deep learning model, trained on data from 33 U.S. sites, to predict when and how fast this PF occurs based on precipitation, soil, and climate data. The model showed that precipitation characteristics (duration, intensity, volume) were the most important predictors of PF. Lower soil sand content/higher clay content was associated with faster water flow, likely due to clay soils forming aggregates and cracks that water moves through rather than infiltrating uniformly. Further analyses based on climate projections suggest that the speed at which PF occurs will become more rapid under future climate scenarios compared to historical simulation. This highlights the need to represent PF in soil water models when assessing future water quality. Key Points The effect of precipitation peak intensity on soil water velocities declined with increasing precipitation intensity Antecedent soil moisture failed to predict preferential flow (PF), contrasting the high predictive power of sand content Climate predictions suggest that soil water velocities through PF paths will increase ∼15% by 2099

Li, Bonan↗

Semidirect computations for transonic flow

A semidirect method, driven by a Poisson solver, was developed for inviscid transonic flow computations. It is an extension of a recently introduced algorithm for solving subsonic rotational flows. Shocks are captured by implementing a form of artificial compressibility. Nonisentropic cases are computed using a shock tracking procedure coupled with the Rankine-Hugoniot relationships. Results are presented for both subsonic and transonic flows. For the test geometry, an unstaggered cascade of 20 percent thick circular arc airfoils at zero angle of attack, shocks are crisply resolved in supercritical situations and the algorithm converges rapidly. In addition, the convergence rate appears to be nearly independent of the entropy and vorticity production at the shock.

Swisshelm, J. M.↗

An Investigation of the Icing and Heated-air De-icing Characteristics of the R-2600-13 Induction System

A laboratory investigation was made on a Holley 1685-HB carburetor mounted on an R-2600-13 supercharger assembly to determine the icing characteristics and the heated-air de-icing requirements of this portion of the B-25D airplane induction system. Icing has been found to be most prevalent at relatively small throttle openings and, consequently, all runs were made at simulated 60-percent normal rated power condition. Icing characteristics were determined during a series of 15-minute runs over a range of inlet-air conditions. For the de-icing investigation severe impact ice was allowed to form in the induction system and the time required for the recovery of 95 percent of the maximum possible air flow at the original throttle setting was then determined for a range of wet-bulb temperatures. Results of these runs showed that ice on the walls of the carburetor adapter and on the rim of the impeller-shroud portion of the supercharger diffuser plate did not affect engine operation at 60-percent normal rated power. Ice that adversely affected the air flow and the fuel-air ratio was formed only on the central web of the carburetor and then only when the inlet air was saturated or contained free moisture in excess of saturation. No serious ice formations were observed at inlet-air temperatures above 66 0 F or with an inlet-air enthalpy greater than 34 Btu per pound. The maximum temperature at. which any trace of icing could be detected was 1110 F with a relative humidity of approximately 28 percent, The air-flow recovery time for emergency de-icing was 0.3 minute for.an enthalpy of 35 Btu per pound or wet-bulb temperature of 68 0 F. Further increase in enthalpy and wet-bulb temperature above these values resulted in very slight improvement in recovery time. The fuel-air ratio restored by a 5-Minute application of heated air was approximately 7 percent less than the initial value for cold-air conditions.

Chapman, Gilbert E.↗

Application of rotor mounted pressure transducers to analysis of inlet turbulence

Miniature pressure transducers installed near the leading edge of a fan blade were used to diagnose the non-uniform flow entering a subsonic tip speed turbofan on a static test stand. The pressure response of the blade to the inlet flow variations was plotted in a form which shows the space-time history of disturbances ingested by the rotor. Also, periodically sampled data values were auto- and cross-correlated as if they had been acquired from fixed hot wire anemometers at 150 equally spaced angles around the inlet. With a clean inlet and low wind, evidence of long, narrow turbulence eddies was easily found both in the boundary layer of the fan duct and outside the boundary layer. The role of the boundary layer was to follow and amplify disturbances in the outer flow. These eddies frequently moved around the inlet with a corkscrew motion as they passed through.

Hanson, D. B.↗

Investigation of Technologies to Improve Condenser Heat Transfer and Performance in a Relevant Coal-Fired Power Plant

Improvements in thermal power-generating plant performance is correlated directly to societal benefits including lower cost of reduced fuel consumption, resulting in lower cost of electricity for the consumer and reduced carbon emissions to the atmosphere. Warm steam exhausted from low-pressure steam turbines is condensed to liquid water on the exterior of thin-walled metal condenser tubes with cooling water passing through the tube interior. The condensation of steam creates a vacuum that supports turbine rotation and the concurrent generation of electricity. This vacuum is optimized when heat transfer across the wall of condenser tube is maximized. Common hindrances to heat transfer include foulants in cooling water that may form and adhere to the interior of condenser tubes, including mineral scale, microbiological films, and particulate deposition. Flowing cooling water may also include a laminar layer at the interior metal surfaces that travels more slowly than bulk water flow, serving to impede heat transfer. On the tube exterior, condensing steam forms an insulating layer of water that flows down the tube and reduces the effectiveness of cooling. Both the interior and exterior barriers to optimal heat transfer may be alleviated to some extent by surface treatments. On the tube interior, hydrophobic coatings may be applied that can reduced the adherence of foulants and of the laminar flow layer to the tube surface. On the tube exterior, hydrophobic coatings or mechanical treatments can be applied that may result in the termination of droplet growth and the departure of droplets from the surface rather than coalescence into a continuous layer of flowing water. Fourteen surface treatments were applied to condenser tubes in this study, including eight interior coatings and six exterior treatments, five of which were coatings and one a microstructural texture. Heat transfer measurement equipment simulating conditions in the condenser of an operating power plant was used to determine heat transfer coefficients by measuring sufficient flow, temperature, water chemistry and other data. Several of the tubes with interior surface treatments showed improvement in heat transfer coefficients compared with a plain (uncoated) tube, and several of the tubes with exterior surface treatments also showed enhanced heat transfer coefficients.

01 COAL, LIGNITE, AND PEAT↗

Nonlinear computation of wing-body-vertical tail-wake flows at low supersonic speeds

A numerical method based on the conservation form of the full potential equation has been applied to the problem of three-dimensional supersonic flows with embedded subsonic regions. The governing equation is cast in a nonorthogonal coordinate system, and the theory of characteristics is used to accurately monitor the type-dependent flow field. A conservative switching scheme is employed to transition from the supersonic marching procedure to a subsonic relaxation algorithm and vice versa. The newly developed computer program can handle arbitrary geometries with fuselage, wing, vertical tail and wake components at combined angle of attack and sideslip. Results are presented for a low supersonic Mach numbers flow over the Shuttle orbiter (including the OMS pods and vertical tail), and for flows over a realistic fighter type configuration. Comparisons with experimental data are shown to be in good agreement for various cases.

Szema, K.-Y.↗

Applicability of linearized-theory attached-flow methods to design and analysis of flap systems at low speeds for thin swept wings with sharp leading edges

Low-speed experimental force and data on a series of thin swept wings with sharp leading edges and leading and trailing-edge flaps are compared with predictions made using a linearized-theory method which includes estimates of vortex forces. These comparisons were made to assess the effectiveness of linearized-theory methods for use in the design and analysis of flap systems in subsonic flow. Results demonstrate that linearized-theory, attached-flow methods (with approximate representation of vortex forces) can form the basis of a rational system for flap design and analysis. Even attached-flow methods that do not take vortex forces into account can be used for the selection of optimized flap-system geometry, but design-point performance levels tend to be underestimated unless vortex forces are included. Illustrative examples of the use of these methods in the design of efficient low-speed flap systems are included.

Carlson, Harry W.↗

Model equation for simulating flows in multistage turbomachinery

A steady, three-dimensional average-passage equation system is derived for use in simulating multistage turbomachinery flows. These equations describe a steady, viscous flow that is periodic from blade passage to blade passage. From this system of equations, various reduced forms can be derived for use in simulating the three-dimensional flow field within multistage machinery. It is suggested that a properly scaled form of the average-passage equation system would provide an improved mathematical model for simulating the flow in multistage machines at design and, in particular, at off-design conditions.

Adamczyk, J. J.↗

Model equation for simulating flows in multistage turbomachinery

A steady, three-dimensional average-passage equation system is derived for use in simulating multistage turbomachinery flows. These equations describe a steady, viscous flow that is periodic from blade passage to blade passage. From this system of equations, various reduced forms can be derived for use in simulating the three-dimensional flow field within multistage machinery. It is suggested that a properly scaled form of the averaged-passage equation system would provide an improved mathematical model for simulating the flow in multistage machines at design and, in particular, at off-design conditions.

Adamczyk, J. J.↗

Probing Supraglacial Debris on Mars 2: Crater Morphology

Lobate debris aprons (LDA), lineated valley fill (LVF), and concentric crater fill (CCF) on Mars, interpreted to bedebris-covered glaciers, possess craters with a suite of distinct interior landforms (called "ring-mold craters")that have been attributed to the presence of glacial ice at depth or surface modification processes. We testedcurrent hypotheses for the formation of ring-mold craters by conducting a comprehensive analysis of the size andmorphology of 16,457 impact craters 125m in diameter formed within glacial deposits in DeuteronilusMensae. Two major groups, bowl-shaped craters and ring-mold craters, are found, with at least nine distinctcrater types. While there is statistical difference in median diameters between these crater types, this differenceis relatively small and is within the estimated uncertainty in diameter measurements and may be related to moreenhanced erosion of the rims of ring-mold craters. Clear degradation sequences are observed, supporting a rolefor post-impact modification in producing at least some of the diversity in crater landforms. The spatial densityof ring-mold craters is also directly correlated with the development of LDA, LVF, and CCF surface textures. Flowlineations cross-cut two ring-mold crater types but they maintain their circular planforms in some cases, suggestingthat the craters initially formed completely within mantling layers deposited after glacial flow hadceased. We also find analogous craters in non-glacial units; glacial ice is therefore not required to form theobserved morphologic diversity. Our observations are most consistent with formation of crater landforms byemplacement and modification of at least two depositional episodes of icy dust (i.e., "mantle"). This mantle wasinitially tens of meters in thickness to support crater formation, and has experienced much downwasting anderosion since emplacement. Derived crater retention ages of 460 Ma for LDA, LVF, and CCF features in the regiontherefore reflect deposition of mantle units and only give a very minimum age for the formation of LDA, LVF, andCCF

Geological↗