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At least 883 records · Page 49

Glycoalkaloids in potato tubers grown under controlled environments

Tuber content of alpha-solanine, alpha-chaconine, and total glycoalkaloids (TGA) was determined for the potato cultivars, Norland, Russet Burbank, and Denali grown under different environmental conditions in growth chambers. The lowest TGA concentrations (0.30 to 0.35 mg g-1 dry tissue) were found in the cv. Norland with 400 micromoles m-2 s-1 photosynthetic photon flux (PPF), 12 h day length, 16 C temperature, and 350 micromoles mol-1 carbon dioxide. The ratio of alpha-chaconine to alpha-solanine was close to 60:40 under all growing conditions, except that it was 50:50 under the low temperature of 12 C. Cultivars responded similarly to environmental conditions although TGA was about 20% greater in cv. Russet Burbank and about 30% greater in Denali compared to Norland. The largest changes in TGA occurred with changes in temperature. In comparison to 16 C, TGA were 40% greater at 12 C, 80% greater at 20 C, and 125% greater at 24 C (0.70 mg g-1 dry weight). The TGA concentration increased from 10 to 25% with an increase in light from 400 to 800 micromoles m-2 s-1 PPF for all three cultivars. TGA increased 20% with extension of the day length from 12 to 24 hr and also increased 20% when carbon dioxide was increased from 350 to 1000 micromoles mol-1. TGA concentrations were not influenced by changes in relative humidity from 50 to 80%. TGA concentrations decreased only slightly in harvests made from 9 to 21 weeks after planting. Variations in TGA among the different growing conditions and cultivars were below 20 mg/100 g fresh weight (approximately 1.0 mg g-1 dry weight) recognized as the upper concentration for food safety. However the results suggest that TGA should be considered when potatoes are grown at temperatures above 20 C.

NASA Discipline Life Support Systems↗

Quality assurance procedures for environmental control and monitoring in plant growth facilities. Report of the North Central Regional 101 Committee on Growth Chamber Use

This report includes procedures for ensuring the quality of the environment provided for plant growth in controlled environment facilities. Biologists and engineers may use these procedures for ensuring quality control during experiments or for ensuring quality control in the design of plant growth facilities. Environmental monitoring prior to and during experiments is included in these procedures. Specific recommendations cover control, acquisition, and calibration for sensor types for the separate parameters of radiation (light), temperature, humidity, carbon dioxide, and air movement.

NASA Discipline Number 61-10↗

Water relations and photosynthesis in the cryptoendolithic microbial habitat of hot and cold deserts

Two cryptoendolithic microbial communities, lichens in the Ross Desert of Antarctica and cyanobacteria in the Negev Desert, inhabit porous sandstone rocks of similar physical structure. Both rock types adsorb water vapor by physical mechanisms unrelated to biological processes. Yet the two microbial communities respond differently to water stress: cryptoendolithic lichens begin to photosynthesize at a matric water potential of -46.4 megaPascals (MPa) [70% relative humidity (RH) at 8 degrees C], resembling thallose desert lichens. Cryptoendolithic cyanobacteria, like other prokaryotes, photosynthesize only at very high matric water potentials [> -6.9 MPa, 90% RH at 20 degrees C].

NASA Discipline Number 52-30↗

Intensity of hydrostimulation for the induction of root hydrotropism and its sensing by the root cap

Roots of Pisum sativum L. and Zea mays L. were exposed to different moisture gradients established by placing both wet cheesecloth (hydrostimulant) and saturated aqueous solutions of various salts in a closed chamber. Atmospheric conditions with different relative humidity (RH) in a range between 98 and 86% RH were obtained at root level, 2 to 3mm from the water-saturated hydrostimulant. Roots of Silver Queen corn placed vertically with the tips down curved sideways toward the hydrostimulant in response to approximately 94% RH but did not respond positively to RH higher than approximately 95%. The positive hydrotropic response increased linearly as RH was lowered from 95 to 90%. A maximum response was observed at RH between 90 and 86%. However, RH required for the induction of hydrotropism as well as the responsiveness differed among plant species used; gravitropically sensitive roots appeared to require a somewhat greater moisture gradient for the induction of hydrotropism. Decapped roots of corn failed to curve hydrotropically, suggesting the root cap as a major site of hydrosensing.

NASA Discipline Plant Biology↗

Hydrotropism in pea roots in a porous-tube water delivery system

Orientation of root growth on earth and under microgravity conditions can possibly be controlled by hydrotropism--growth toward a moisture source in the absence of or reduced gravitropism. A porous-tube water delivery system being used for plant growth studies is appropriate for testing this hypothesis since roots can be grown aeroponically in this system. When the roots of the agravitropic mutant pea ageotropum (Pisum sativum L.) were placed vertically in air of 91% relative humidity and 2 to 3 mm from the water-saturated porous tube placed horizontally, the roots responded hydrotropically and grew in a continuous arch along the circular surface of the tube. By contrast, normal gravitropic roots of Alaska' pea initially showed a slight transient curvature toward the tube and then resumed vertical downward growth due to gravitropism. Thus, in microgravity, normal gravitropic roots could respond to a moisture gradient as strongly as the agravitropic roots used in this study. Hydrotropism should be considered a significant factor responsible for orientation of root growth in microgravity.

NASA Program CELSS↗

Temperature sensing by primary roots of maize

Zea mays L. seedlings, grown on agar plates at 26 degrees C, reoriented the original vertical direction of their primary root when exposed to a thermal gradient applied perpendicular to the gravity vector. The magnitude and direction of curvature can not be explained simply by either a temperature or a humidity effect on root elongation. It is concluded that primary roots of maize sense temperature gradients in addition to sensing the gravitational force.

NASA Program Space Biology↗

Water relations, thallus structure and photosynthesis in Negev Desert lichens

The role of lichen thallus structure in water relations and photosynthesis was studied in Ramalina maciformis (Del.) Bory and Teloschistes lacunosus (Rupr.) Sav. Water-vapour adsorption and photosynthesis are dependent upon thallus integrity and are significantly lower in crushed thalli. Cultured phycobiont (Trebouxia sp.) cells are capable of photosynthesis over the same relative humidity range (> 80% RH) as are intact lichens. Thus, water-vapour adsorption by the thallus and physiological adaptation of the phycobiont contribute to the ability of these lichens to photosynthesize in an arid environment. Despite differences in their anatomical structure and water-uptake characteristics, their CO2 incorporation is similar. The two lichens use liquid water differently and they occupy different niches.

Non-NASA Center↗

Efficiency and temperature dependence of water removal by membrane dryers

The vapor pressure of water in equilibrium with sorption sites within a Nafion membrane is given by log P(WN) = -3580/T + 10.01, where P(WN) is expressed in Torr and T is the membrane temperature, in kelvin. The efficiency of dryers based on selective permeation of water through Nafion can thus be enhanced by cooling the membrane. Residual water in effluents exceeds equilibrium levels if insufficient time is allowed for water to diffuse to the membrane surface as gas passes through the dryer. For tubular configurations, this limitation can be avoided if L > or = Fc(10(3.8)/120 pi D), where L is the length of the tubular membrane, in centimeters, Fc is the gas flow rate, in mL/ min, and D is the diffusion coefficient for water in the carrier gas at the operating temperature of the dryer, in cm2/s. An efficient dryer that at room temperature dries gas to a dew point of -61 degrees C is described; the same dryer maintained at 0 degrees C yields a dew point of -80 degrees C and removes water as effectively as Mg(ClO4)2 or a dry ice/acetone slush. The use of Nafion membranes to construct devices capable of delivering gas streams with low but precisely controlled humidities is discussed.

Non-NASA Center↗

[Evaluation of super dwarf wheat growth and development in greenhouse "Svet" during cultivation in inhabited pressurized chamber]

Goals of the 3-month experiment GREENHOUSE using the equipment of greenhouse SVET (ECO-PSY-95) were to feature growth and development of wheat through the entire cycle of ontogeny under the maximally mimicked MIR environment, and to try out the procedures and timeline of space experiment GREENHOUSE-2 as a part of the fundamental biology investigations within the MIR/NASA space science program. Irradiation intensity (PAR) was 65 W/m2 and 38 W/m2 in the experiment and laboratory control, respectively. Values of other environmental parameters were MIR average (18-25 degrees C, relative air humidity in the interval between 40% and 75%, total gas pressure of about 660 to 860 mm Hg, partial oxygen pressure within the range from 140 to 200 mm Hg, partial carbon dioxide pressure up to 7 mm Hg). Experimental results showed that wheat cultivation in inhabited chamber under a modified lighting unit providing greater irradiation of the crop area produced more plant mass although seed production dropped. Low grain content in ears could be the aftermath of the gaseous trace contaminants in the chamber atmosphere.

NASA Discipline Life Support Systems↗

Temperature and moisture conditions for life in the extreme arid region of the Atacama desert: four years of observations including the El Nino of 1997-1998

The Atacama along the Pacific Coast of Chile and Peru is one of the driest and possibly oldest deserts in the world. It represents an extreme habitat for life on Earth and is an analog for life in dry conditions on Mars. We report on four years (September 1994-October 1998) of climate and moisture data from the extreme arid region of the Atacama. Our data are focused on understanding moisture sources and their role in creating suitable environments for photosynthetic microorganisms in the desert surface. The average air temperature was 16.5 degrees C and 16.6 degrees C in 1995 and 1996, respectively. The maximum air temperature recorded was 37.9 degrees C, and the minimum was -5.7 degrees C. Annual average sunlight was 336 and 335 W m(-2) in 1995 and 1996, respectively. Winds averaged a few meters per second, with strong fohn winds coming from the west exceeding 12 m s(-1). During our 4 years of observation there was only one significant rain event of 2.3 mm, which occurred near midnight local time. We suggest that this event was a rainout of a heavy fog. It is of interest that the strong El Nino of 1997-1998 brought heavy rainfall to the deserts of Peru, but did not bring significant rain to the central Atacama in Chile. Dew occurred at our station frequently following high nighttime relative humidity, but is not a significant source of moisture in the soil or under stones. Groundwater also does not contribute to surface moisture. Only the one rain event of 2.3 mm resulted in liquid water in the soil and beneath stones for a total of only 65-85 h over 4 years. The paucity of liquid water under stones is consistent with the apparent absence of hypolithic (under-stone) cyanobacteria, the only known primary producers in such extreme deserts.

Biogenesis↗

Microbial Characterization of Free Floating Condensate Aboard the Mir Space Station

Three samples of humidity condensate that had accumulated behind panels aboard the Russian space station Mir were collected and returned to earth for analysis. As these floating masses of liquid come into contact with the astronauts and the engineering systems, they have the potential to affect both crew health and systems performance. Using a combination of culturing techniques, a wide variety of organisms were isolated included Escherichia coli, Serratia marcescens, and a presumed Legionella species. In addition, microscopic analysis indicated the presence of protozoa, dust mites, and spirochetes. These findings suggest the need for more comprehensive microbial analysis of the environment through the use of new methodologies to allow a more thorough risk assessment of spacecraft. Copyright 2004 Springer-Verlag.

Spacecraft↗

LaRC SmartLab Apps For Instrument Control and Data Processing: Laboratory Environment Monitor

The LaRC SmartLab applications are a series of software tools to greatly enhance researcher efficiency by streamlining and automating workflows. Python scripts and applications are increasingly being used in scientific workflows, including for instrument control and data processing. Interactive Python scripting environments such as JupyterLab provide powerful tools for using Python. In some use cases, the development of standalone applications with dedicated graphical user interfaces can enhance the utility of the code and open it up to more users, including non-programmers. Here, we describe a Python based application for communicating with, and displaying data from, iTHX Temperature, Humidity, and Dew Point probes. We discuss the set up and use of the application as well as its implementation. We also highlight the use of Simulated probes to enable users and developers to familiarize with or debug the application, even when they do not have access to the physical hardware in the laboratory.

LaRC SmartLab↗

The Historical Greenland Climate Network (GC-Net) Curated and Augmented Level-1 Dataset

The Greenland Climate Network (GC-Net) consists of 31 automatic weather stations (AWSs) at 30 sites across the Greenland Ice Sheet. The first site was initiated in 1990, and the project has operated almost continuously since 1995 under the leadership of the late Konrad Steffen. The GC-Net AWS measured air temperature, relative humidity, wind speed, atmospheric pressure, downward and reflected shortwave irradiance, net radiation, and ice and firn temperatures. The majority of the GC-Net sites were located in the ice sheet accumulation area (17 AWSs), while 11 AWSs were located in the ablation area, and two sites (three AWSs) were located close to the equilibrium line altitude. Additionally, three AWSs of similar design to the GC-Net AWS were installed by Konrad Steffen's team on the Larsen C ice shelf, Antarctica. After more than 3 decades of operation, the GC-Net AWSs are being decommissioned and replaced by new AWSs operated by the Geological Survey of Denmark and Greenland (GEUS). Therefore, making a reassessment of the historical GC-Net AWS data is necessary. We present a full reprocessing of the historical GC-Net AWS dataset with increased attention to the filtering of erroneous measurements, data correction and derivation of additional variables: continuous surface height, instrument heights, surface albedo, turbulent heat fluxes, and 10 m ice and firn temperatures. This new augmented GC-Net level-1 (L1) AWS dataset is now available at https://doi.org/10.22008/FK2/VVXGUT (Steffen et al., 2023) and will continue to be refined. The processing scripts, latest data and a data user forum are available at https://github.com/GEUS-Glaciology-and-Climate/GC-Net-level-1-data-processing (last access: 30 November 2023). In addition to the AWS data, a comprehensive compilation of valuable metadata is provided: maintenance reports, yearly pictures of the stations and the station positions through time. This unique dataset provides more than 320 station years of high-quality atmospheric data and is available following FAIR (findable, accessible, interoperable, reusable) data and code practices.

Greenland Climate Network↗

The Global Seasonal Relationship Between Satellite-Observed Cold Pools and Rainfall

Convective cold pools are important modulators of the onset and evolution of deep convection in the tropics. This work leverages a dataset derived from the Advanced Scatterometer (ASCAT) satellite instrument by Garg et al. (2020) to quantify seasonal variations in cold pool activity and their relationship to deep convection across tropical ocean basins. The dataset identifies gradient features (GFs) in the surface wind field, which have been shown to serve as reliable proxies for the boundaries of atmospheric cold pools. We examine the relationship between GFs and climatologies of precipitation, column relative humidity (CRH), and bulk vertical wind shear. We also collocate GFs with precipitation and CRH. High GF frequency, precipitation, and CRH coincide in many regions of the tropics, consistent with our understanding of the physical connections between precipitation and cold pool generation. On the other hand, climatological bulk wind shear is often low in convective regions, and there is a weak inverse correlation between GF frequency and bulk wind shear, while our prior expectation might have been that shear promotes cold pool formation. Compared to GF frequency, GF size shows a weaker relationship with the convective environment, with some of the largest GF sizes occurring at lower CRH values for a given rainfall rate. In a few exceptional regions and seasons, such as the Indian Ocean in northern hemisphere summer, the region of greatest precipitation does not coincide with the region of greatest GF frequency. These cases also have very high seasonal mean CRH, suggesting that in these regions cold pool formation is suppressed by reduced evaporation of precipitation.

Convective cold pools↗

SPACECRAFT LIFE SUPPORT SYSTEMS

Requirements of life support for the crew members considered in relation to man's metabolic requirements Appropriate methods and equipment are related to these characteristics of various manned space missions. Among the provisions required for maintaining physical well-being are systems for (1) the removal of carbon dioxide, (2) the reclamation and supplying of oxygen, (3) the control of temperature, humidity, and toxic gases, (4) the reclamation of water, (5) the management of wastes, and (6) the supplying of food. Some of the research being performed at the Langley Research Center with life support systems is discussed and the research objectives are stressed. Descriptions are given of the various types of equipment used in this research, together with criteria for system evaluations.

MANNED SPACECRAFT↗

NIMBUS TESTING

Humidity, vibration, acceleration and vacuum thermal environmental testing of the nimbus meteorological satellite

ENVIRONMENTAL TESTING↗