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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 865 records · Page 48
All sourcesNASA NTRSDOE OSTI
NASA NTRS2015

Exposing Microorganisms in the Stratosphere for Planetary Protection Project

Earths stratosphere is similar to the surface of Mars: rarified air which is dry, cold, and irradiated. E-MIST is a balloon payload that has 4 independently rotating skewers that hold known quantities of spore-forming bacteria isolated from spacecraft assembly facilities at NASA. Knowing the survival profile of microbes in the stratosphere can uniquely contribute to NASA Planetary Protection for Mars.Objectives 1. Collect environmental data in the stratosphere to understand factors impacting microbial survival. 2. Determine of surviving microbes (compared to starting quantities). 3. Examine microbial DNA mutations induced by stratosphere exposure.

Technology Portfolio System↗
NASA NTRS2018

Microbial Monitoring and the Risk of Infectious Disease Aboard the International Space Station

The International Space Station (ISS) is a semi-closed habitat in low Earth orbit with environmental conditions provided by an advanced life support system that controls temperature and recycles air and most of the potable water. The crew's activities, such as eating, sleeping, hygiene, and laboratory research, are performed in relatively close proximity. Research in the laboratory includes a myriad of experiments, including those with rodents, plants, and pathogenic microorganisms. Despite these conditions, in-flight monitoring of ISS indicates that the microbial diversity is similar to homes on earth. Accordingly, the crew is generally very healthy, however infectious disease does occur and potential routes of infection by obligate and opportunistic pathogens cannot be completely prevented. Determining the extent of this risk is further complicated, as microorganisms can alter their characteristics in response to spaceflight culture, as exemplified by the increase in virulence of the enteric pathogen Salmonella enterica Typhimurium during spaceflight compared to otherwise identical cultures grown on Earth. Taken together, these factors suggest a need for continued microbiological monitoring and research to understand and mitigate the risk of infectious disease during long duration missions.

Ott, C. Mark↗
DOE OSTI2020

Measurement of free chlorine levels in water using potentiometric responses of biofilms and applications for monitoring and managing the quality of potable water

We report residual free chlorine is not monitored continuously at scale in drinking water distribution systems because existing real-time sensor technologies require frequent maintenance, cleaning, and calibration, which makes these products too costly to be used throughout a distribution system. As a result, current measurement approaches require manual sampling, which is not feasible for the consistent monitoring of free chlorine because chlorine concentrations vary significantly throughout pipeline distribution and over time and space. This research presents an alternative and cost-effective method of predicting free chlorine levels in drinking water using graphite electrodes coated with naturally grown microbial biofilms. This Microbial Potentiometric Sensor (MPS) array was installed in a Continuously Mixed Batch Reactor (CMBR), and drinking water containing variable free chlorine concentrations. The chlorine concentrations were introduced in a controlled manner, and the MPS signals were monitored over time. MPS signals were measured from the change in Open Circuit Potential (OCP) across the MPS array in real-time. An empirically derived relationship between the normalized change in OCP and free chlorine was established by fitting individual and average MPS data to a decaying exponential growth function in order to predict free chlorine levels. The results show that free chlorine can be predicted with reasonable accuracy, with model validation showing an average absolute error of ±0.09 ppm below 1.1 ppm and ±0.30 ppm between 1.1 and 2.7 ppm. However, the accuracy of predictions was reduced at higher free chlorine levels. The researchers conclude that MPS systems may benefit drinking water distribution systems by measuring free chlorine. These advantages of the MPS are especially pronounced in the developing world because this system is inexpensive and does not require routine maintenance or cleaning. The system relies on a naturally forming and regenerating biofilm and an inexpensive potentiometric meter to produce stable measurements.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2022

Edaphic controls on genome size and GC content of bacteria in soil microbial communities

Nutrient limitation has been shown to reduce bacterial genome size and influence nucleotide composition; however, much of this work has been conducted in marine systems and the factors which shape soil bacterial genomic traits remain largely unknown. Here, for this work, we determined average genome size, GC content, codon usage, and amino acid content from 398 soil metagenomes across a broad geographic range and used machine-learning to determine the environmental parameters that most strongly explain the distribution of these traits. We found that genomic trait averages were most related to pH, which we suggest is primarily due to the correlation of pH with several environmental parameters, particularly soil carbon content. Low pH soils had higher carbon to nitrogen ratios (C:N) and tended to have communities with lower GC content and larger genomes, potentially a response to increased physiological stress and a requirement for metabolic diversity. Conversely, communities in high pH and low soil C:N had smaller genomes and higher GC content—indicating potential resource driven selection against AT base pairs, which have a higher C:N than GC base pairs. Similarly, we found that nutrient conservation also applied to amino acid stoichiometry, where bacteria in soils with low C:N ratios tended to code for amino acids with lower C:N. Together, these relationships point towards fundamental mechanisms that underpin genome size, and nucleotide and amino acid selection in soil bacteria.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Drought increases microbial allocation to stress tolerance but with few tradeoffs among community-level traits

We leverage the Kellogg Biological Station Long Term Ecological Research Main Cropping System Experiment (KBS MCSE) field experiment to test trade-offs among high growth yield (Y), resource acquisition (A), and stress tolerance (S) traits using metagenomic data from plots with different land use, drought manipulation, and C addition.

Jones, Jennifer M. [WK Kellogg Biological Station,↗
NASA NTRS2002

Composition and physiological profiling of sprout-associated microbial communities

The native microfloras of various types of sprouts (alfalfa, clover, sunflower, mung bean, and broccoli sprouts) were examined to assess the relative effects of sprout type and inoculum factors (i.e., sprout-growing facility, seed lot, and inoculation with sprout-derived inocula) on the microbial community structure of sprouts. Sprouts were sonicated for 7 min or hand shaken with glass beads for 2 min to recover native microfloras from the surface, and the resulting suspensions were diluted and plated. The culturable fraction was characterized by the density (log CFU/g), richness (e.g., number of types of bacteria), and diversity (e.g., microbial richness and evenness) of colonies on tryptic soy agar plates incubated for 48 h at 30 degrees C. The relative similarity between sprout-associated microbial communities was assessed with the use of community-level physiological profiles (CLPPs) based on patterns of utilization of 95 separate carbon sources. Aerobic plate counts of 7.96 +/- 0.91 log CFU/g of sprout tissue (fresh weight) were observed, with no statistically significant differences in microbial cell density, richness, or diversity due to sprout type, sprout-growing facility, or seed lot. CLPP analyses revealed that the microbial communities associated with alfalfa and clover sprouts are more similar than those associated with the other sprout types tested. Variability among sprout types was more extensive than any differences between microbial communities associated with alfalfa and clover sprouts from different sprout-growing facilities and seed lots. These results indicate that the subsequent testing of biocontrol agents should focus on similar organisms for alfalfa and clover, but alternative types may be most suitable for the other sprout types tested. The inoculation of alfalfa sprouts with communities derived from various sprout types had a significant, source-independent effect on microbial community structure, indicating that the process of inoculation alters the dynamics of community development regardless of the types of organisms involved.

NASA Center KSC↗
NASA NTRS2009

Crew Exploration Vehicle (CEV) Potable Water System Verification Description

The Crew Exploration Vehicle (CEV), also known as Orion, will ferry a crew of up to six astronauts to the International Space Station (ISS), or a crew of up to four astronauts to the moon. The first launch of CEV is scheduled for approximately 2014. A stored water system on the CEV will supply the crew with potable water for various purposes: drinking and food rehydration, hygiene, medical needs, sublimation, and various contingency situations. The current baseline biocide for the stored water system is ionic silver, similar in composition to the biocide used to maintain quality of the water transferred from the Orbiter to the ISS and stored in Contingency Water Containers (CWCs). In the CEV water system, the ionic silver biocide is expected to be depleted from solution due to ionic silver plating onto the surfaces of the materials within the CEV water system, thus negating its effectiveness as a biocide. Since the biocide depletion is expected to occur within a short amount of time after loading the water into the CEV water tanks at the Kennedy Space Center (KSC), an additional microbial control is a 0.1 micron point of use filter that will be used at the outlet of the Potable Water Dispenser (PWD). Because this may be the first time NASA is considering a stored water system for longterm missions that does not maintain a residual biocide, a team of experts in materials compatibility, biofilms and point of use filters, surface treatment and coatings, and biocides has been created to pinpoint concerns and perform testing to help alleviate those concerns related to the CEV water system. Results from the test plans laid out in the paper presented to SAE last year (Crew Exploration Vehicle (CEV) Potable Water System Verification Coordination, 2008012083) will be detailed in this paper. Additionally, recommendations for the CEV verification will be described for risk mitigation in meeting the physicochemical and microbiological requirements on the CEV PWS.

Peterson, Laurie↗
NASA NTRS2009

Life in Ice: Implications to Astrobiology

During the 2008 Tawani International Expedition Schirmacher Oasis/Lake Untersee Antarctica Expedition, living and instantly motile bacteria were found in freshly thawed meltwater from ice of the Schirmacher Oasis Lakes, the Anuchin Glacier ice and samples of the that perennial ice sheet above Lake Untersee. This phenomenon of living bacteria encased in ice had previously been observed in the 32,000 year old ice of the Fox Tunnel. The bacteria found in this ice included the strain FTR1T which was isolated and published as valid new species (Carnobacterium pleistocenium) the first validly published living Pleistocene organism still alive today. Living bacteria were also extracted from ancient ice cores from Vostok, Antarctica. The discovery that many strains of bacteria are able to survive and remain alive while frozen in ice sheets for long periods of time may have direct relevance to Astrobiology. The abundance of viable bacteria in the ice sheets of Antarctica suggests that the presence of live bacteria in ice is common, rather than an isolated phenomenon. This paper will discuss the results of recent studies at NSSTC of bacteria cryopreserved in ice. This paper advances the hypothesis that cryopreserved cells, and perhaps even viable bacterial cells, may exist today--frozen in the water-ice of lunar craters, the Polar Caps or craters of Mars; or in the permafrost of Mars; ice and rocks of comets or water bearing asteroids; or in the frozen crusts of the icy moons of Jupiter and Saturn. The existence of bacterial life in ice suggests that it may not be necessary to drill through a thick ice crust to reach liquid water seas deep beneath the icy crusts of Europa, Ganymede and Enceladus. The presence of viable bacteria in the ice of the Earth s Polar Caps suggests that the possibility that cryo-panspermia (i.e., the trans-planetary transfer of microbial life by impact ejection/spallation of bacteria-rich polar ice masses) deserves serious consideration and study as a possible natural phenomenon of the solar system that may have played a profoundly important role in the Origin of Life on Earth and the Distribution of Life in the Cosmos. The paper concludes with a consideration of the protective properties of ice by absorption of UV-B, UV-C, h-rays, gamma-rays and the high energy proton environment of the Jupiter Radiation Belt. A proposed instrument that may provide additional data on the potential survivability of microbial extremophiles encased in ice and subjected to the simulated space environment will be briefly described.

Hoover, Richard B.↗
DOE OSTI2021

BETO 2021 Peer Review - Biological Upgrading of Sugars (BUS) 2.3.2.105

The Biological Upgrading of Sugars (BUS) project directly targets the anaerobic conversion of lignocellulosic feedstocks into intermediate molecules readily upgradeable to fuel precursors. Recent efforts on the BUS project have a particular emphasis on the biological production of butyric acid, an intermediate that can be readily upgraded to sustainable aviation fuel, diesel blend-stocks, and high value chemicals. The BUS project approaches this direction through a combination of strain engineering, fermentation process engineering, development of novel separations technologies, and the design and build of pilot scale systems. Our ultimate project goal is to develop an integrated cost-effective process at pilot scale to achieve DOE's MYPP targets of $2.50/GGE. The major thrust of the BUS project over the last project cycle was on the development of integrated processes surrounding the anaerobic production of carboxylic acids using diverse Clostridium species. We developed and expanded genetic tools for several Clostridium species and rewired microbial metabolism in an attempt to maximize substrate utilization and flux towards butyric acid. We designed and built novel bioreactors with an in situ product recovery system enabling the biological production and recovery of highly purified acids. We leveraged this system to generate 100s of grams of acid from corn stover hydrolysate. In this presentation we highlight data surrounding our proposed process and accompanying results from technoeconomic and life-cycle analyses of our integrated process. Finally, we detail plans of our pilot scale reactor system that is in process and discuss our future routes towards achieving economically viable and sustainable diesel and jet blendstocks.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
DOE OSTI2021

Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil

The microbial and molecular characterization of the ectorhizosphere is an important step towards developing a more complete understanding of how the cultivation of biofuel crops can be undertaken in nutrient poor environments. The ectorhizosphere of Setaria is of particular interest because the plant component of this plant-microbe system is an important agricultural grain crop and a model for biofuel grasses. Importantly, Setaria lends itself to high throughput molecular studies. As such, we have identified important intra- and interspecific microbial and molecular differences in the ectorhizospheres of three geographically distant Setaria italica accessions and their wild ancestor S . viridis . All were grown in a nutrient-poor soil with and without nutrient addition. To assess the contrasting impact of nutrient deficiency observed for two S . italica accessions, we quantitatively evaluated differences in soil organic matter, microbial community, and metabolite profiles. Together, these measurements suggest that rhizosphere priming differs with Setaria accession, which comes from alterations in microbial community abundances, specifically Actinobacteria and Proteobacteria populations. When globally comparing the metabolomic response of Setaria to nutrient addition, plants produced distinctly different metabolic profiles in the leaves and roots. With nutrient addition, increases of nitrogen containing metabolites were significantly higher in plant leaves and roots along with significant increases in tyrosine derived alkaloids, serotonin, and synephrine. Glycerol was also found to be significantly increased in the leaves as well as the ectorhizosphere. These differences provide insight into how C 4 grasses adapt to changing nutrient availability in soils or with contrasting fertilization schemas. Gained knowledge could then be utilized in plant enhancement and bioengineering efforts to produce plants with superior traits when grown in nutrient poor soils.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRSDate not supplied

Electron Microscopy of Microbial Biomass

As humans move to long term stays in space their microbes move with them. This poses problems as the microbes can colonize various life support systems, creating biofilms that can grow and spread, which in turn causes obstructions and ultimately degrades function of these systems. MSFC currently has no protocol in place for the Scanning Electron Microscope (SEM) imaging of microbes.

Samantha Marcella↗
DOE OSTI2020

The Role of Retardation, Attachment and Detachment Processes during Microbial Coal-Bed Methane Production after Organic Amendment

Microbially enhanced coal-bed methane could allow for a more sustainable method of harvesting methane from un-mineable coaldbeds. The model presented here is based on a previously validated batch model; however, this model system is based on upflow reactor columns compared to previous experiments and now includes flow, transport and reactions of amendment as well as intermediate products. The model implements filtration and retardation effects, biofilm decay, and attachment and detachment processes of microbial cells due to shear stress. The model provides additional insights into processes that cannot be easily observed in experiments. This study improves the understanding of complex and strongly interacting processes involved in microbially enhanced coal-bed methane production and provides a powerful tool able to model the entire process of enhancing methane production and transport during microbial stimulation.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Intensified Soil Moisture Extremes Decrease Soil Organic Carbon Decomposition: A Mechanistic Modeling Analysis

Earth system models have predicted that there will be more frequent and severe precipitation and drought events in terrestrial ecosystems. Microbially mediated decomposition of soil organic carbon (SOC) tends to increase as soils wet and decrease as soils dry. However, the long-term SOC change under intensified moisture extremes remains poorly known as it depends on the frequency and intensity of soil drying and wetting. In this study, we explored long-term SOC dynamics under scenarios of alternating drying-wetting cycles using the Microbial-ENzyme Decomposition model, a mechanistic microbial model. The model was parameterized with 11 years of observations from a temperate deciduous broadleaf forest site, showing satisfactory model performance in both model calibration (R 2 = 0.67) and validation (R 2 = 0.69) against heterotrophic respiration. We then used the model to simulate the long-term SOC dynamics under five scenarios of alternating drying-wetting cycles with different frequencies and severities over a period of 100 years. Results showed that the changes in active microbial biomass C and the corresponding turnover rates of SOC pools were more sensitive to soil drying than soil wetting. As a result, the cumulative soil carbon emission from microbial respiration decreased by 433.7 g C m -2 after the 100-year simulation in the highest frequency and intensity moisture scenario, but was not significantly affected by the lowest frequency and intensity scenario. This study emphasizes the nonlinear response of SOC decomposition to soil moisture changes, which causes decreased decomposition by microbes under drying that is, not compensated by increased decomposition under wetting conditions.

58 GEOSCIENCES↗
DOE OSTI2022

MIDAS2: Metagenomic Intra-species Diversity Analysis System

The Metagenomic Intra-Species Diversity Analysis System (MIDAS) is a scalable metagenomic pipeline that identifies single nucleotide variants (SNVs) and gene copy number variants in microbial populations. Here, we present MIDAS2, which addresses the computational challenges presented by increasingly large reference genome databases, while adding functionality for building custom databases and leveraging paired-end reads to improve SNV accuracy. This fast and scalable reengineering of the MIDAS pipeline enables thousands of metagenomic samples to be efficiently genotyped.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Metabolic multistability and hysteresis in a model aerobe-anaerobe microbiome community

Major changes in the microbiome are associated with health and disease. Some microbiome states persist despite seemingly unfavorable conditions, such as the proliferation of aerobe-anaerobe communities in oxygen-exposed environments in wound infections or small intestinal bacterial overgrowth. Mechanisms underlying transitions into and persistence of these states remain unclear. Using two microbial taxa relevant to the human microbiome, we combine genome-scale mathematical modeling, bioreactor experiments, transcriptomics, and dynamical systems theory to show that multistability and hysteresis (MSH) is a mechanism describing the shift from an aerobe-dominated state to a resilient, paradoxically persistent aerobe-anaerobe state. We examine the impact of changing oxygen and nutrient regimes and identify changes in metabolism and gene expression that lead to MSH and associated multi-stable states. In such systems, conceptual causation-correlation connections break and MSH must be used for analysis. Using MSH to analyze microbiome dynamics will improve our conceptual understanding of stability of microbiome states and transitions between states.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1971

Reusable anaerobic system for microbiological studies - A concept

Simple, low cost system consists of semirigid incubation chamber with clear Teflon window, airtight zipper and internal compartments for petri dishes or microbial plates. Device interests schools, medical laboratories, and manufacturers of biological and pharmaceutical supplies.

Murawczyk, C.↗
NASA NTRS2010

Bacteria Provide Cleanup of Oil Spills, Wastewater

Through Small Business Innovation Research (SBIR) contracts with Marshall Space Flight Center, Micro-Bac International Inc., of Round Rock, Texas, developed a phototrophic cell for water purification in space. Inside the cell: millions of photosynthetic bacteria. Micro-Bac proceeded to commercialize the bacterial formulation it developed for the SBIR project. The formulation is now used for the remediation of wastewater systems and waste from livestock farms and food manufacturers. Strains of the SBIR-derived bacteria also feature in microbial solutions that treat environmentally damaging oil spills, such as that resulting from the catastrophic 2010 Deepwater Horizon oil rig explosion in the Gulf of Mexico.

Source record↗
NASA NTRS2014

Use Of The Operational Air Quality Monitor (AQM) For In-Flight Water Testing Project

A primary requirement for manned spaceflight is Environmental Health which ensures air and water contaminants, acoustic profiles, microbial flora, and radiation exposures within the cabin are maintained to levels needed for crew health and for vehicle system functionality. The reliance on ground analyses of returned samples is a limitation in the current environmental monitoring strategy that will prevent future Exploration missions beyond low-Earth orbit. This proposal attempts to address this shortcoming by advancing in-flight analyses of water and air. Ground analysis of in-flight, air and water samples typically employ vapor-phase analysis by gas chromatography-mass spectrometry (GC-MS) to identify and quantify organic compounds present in the samples. We envision the use of newly-developed direct ionization approaches as the most viable avenue leading towards an integrated analytical platform for the monitoring of water, air, and, potentially bio-samples in the cabin environment. Development of an in-flight instrument capable of analyzing air and water samples would be the logical next step to meeting the environmental monitoring needs of Exploration missions. Currently, the Air Quality Monitor (AQM) on-board ISS provides this specific information for a number of target compounds in the air. However, there is a significant subset of common target compounds between air and water. Naturally, the following question arises, "Can the AQM be used for both air and water quality monitoring?" Previous directorate-level IR&D funding led to the development of a water sample introduction method for mass spectrometry using electrothermal vaporization (ETV). This project will focus on the integration of the ETV with a ground-based AQM. The capabilities of this integrated platform will be evaluated using a subset of toxicologically important compounds.

Macatangay, Ariel↗