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At least 865 records · Page 48

Both necrosis and apoptosis contribute to HIV-1-induced killing of CD4 cells

BACKGROUND: Data currently available on HIV-1-induced cytopathology is unclear regarding the mechanism of cell killing. OBJECTIVE: To clarify the extent to which apoptosis or necrosis is involved in HIV-1-induced cell death in view of conflicting existing data. METHODS: T lymphoblastoid cells or peripheral blood mononuclear cells were infected by various strains of HIV-1 and the numbers of apoptotic or necrotic cells were quantified at various times after infection using video-image analysis techniques; the results were compared with the amount of fragmented DNA using a quantitative method. Measurement of mitochondrial transmembrane potential (deltapsi(m)) and intracellular calcium concentrations [Ca2+]i was performed with fluorescent probes and fluorescence concentration analysis (FCA). RESULTS: Although lymphoblastoid and monocytoid cells acutely infected by HIV-1 had increased levels of fragmented DNA, a marker of apoptotic cell death, few (<12%) had condensed chromatin and fragmented nuclei, the morphological features of apoptosis. The predominant alterations in acutely infected cells were distended endoplasmic reticulum and abnormal mitochondria; these ultrastructural changes are consistent with necrosis, although some infected cells simultaneously displayed features of both necrosis and apoptosis. Viability of cells persistently infected by HIV-1 was only minimally reduced from that of uninfected cells. This reduction was accounted for by an increased propensity of the persistently infected cells to die by apoptosis. Alterations in [Ca2+]i and deltapsi(m) occurred in both acutely and persistently infected cells. CONCLUSION: Both necrosis and apoptosis contribute to HIV-1-induced killing of CD4 cells.

Non-NASA Center↗

Compositional Diversity of the Vestan Regolith Derived from Howardite Compositions and Dawn VIR Spectra

Howardite, eucrite and diogenite meteorites likely come from asteroid 4 Vesta [1]. Howardites - physical mixtures of eucrites and diogenites - are of two subtypes: regolithic howardites were gardened in the true regolith; fragmental howardites are simple polymict breccias [2]. The Dawn spacecraft imaged the howarditic surface of Vesta with the visible and infrared mapping spectrometer (VIR) resulting in qualitative maps of the distributions of distinct diogenite-rich and eucrite-rich terranes [3, 4]. We are developing a robust basis for quantitative mapping of the distribution of lithologic types using spectra acquired on splits of well-characterized howardites [5, 6]. Spectra were measured on sample powders sieved to <75 μm in the laboratories of the Istituto di Astrofisica e Planetologia Spaziali and Brown University. Data reduction was done using the methods developed to process Dawn VIR spectra [4]. The band parameters for the ~1 and ~2 μm pyroxene absorption features (hereafter BI and BII) can be directly compared to Dawn VIR results. Regolithic howardites have shallower BI and BII absorptions compared to fragmental howardites with similar compositions. However, there are statistically significant correlations between Al or Ca contents and BI or BII center wavelengths regardless of howardite subtype. Diogenites are poor in Al and Ca while eucrites are rich in these elements. The laboratory spectra can thus be directly correlated with the percentage of eucrite material contained in the howardites. We are using these correlations to quantitatively map Al and Ca distributions, and thus the percentage of eucritic material, in the current regolith of Vesta.

Mittlefehldt, D. W.↗

Ionosphere Plasma State Determination in Low Earth Orbit from International Space Station Plasma Monitor

A plasma diagnostic package is deployed on the International Space Station (ISS). The system - a Floating Potential Measurement Unit (FPMU) - is used by NASA to monitor the electrical floating potential of the vehicle to assure astronaut safety during extravehicular activity. However, data from the unit also reflects the ionosphere state and seems to represent an unutilized scientific resource in the form of an archive of scientific plasma state data. The unit comprises a Floating Potential probe and two Langmuir probes. There is also an unused but active plasma impedance probe. The data, at one second cadence, are collected, typically for a two week period surrounding extravehicular activity events. Data is also collected any time a visiting vehicle docks with ISS and also when any large solar events occur. The telemetry system is unusual because the package is mounted on a television camera stanchion and its data is impressed on a video signal that is transmitted to the ground and streamed by internet to two off center laboratory locations. The data quality has in the past been challenged by weaknesses in the integrated ground station and distribution systems. These issues, since mid‐2010, have been largely resolved and the ground stations have been upgraded. Downstream data reduction has been developed using physics based modeling of the electron and ion collecting character in the plasma. Recursive algorithms determine plasma density and temperature from the raw Langmuir probe current voltage sweeps and this is made available in real time for situational awareness. The purpose of this paper is to describe and record the algorithm for data reduction and to show that the Floating probe and Langmuir probes are capable of providing long term plasma state measurement in the ionosphere. Geophysical features such as the Appleton anomaly and high latitude modulation at the edge of the Auroral zones are regularly observed in the nearly circular, 51 deg inclined, 400 km altitude ISS orbit. Evidence of waves in the ion collection current data is seen in geographic zones known to exhibit the spread‐F phenomenon. An anomaly in the current collection characteristic of the cylindrical probe appears also too be organized by the geomagnetic field.

Kramer, Leonard↗

Food Sanitation Device for Use in Microgravity

EDEN is a transportation/orbital habitat that carries large amounts of crew members, food, and supplies from Earth to Mars and back, but can also act as a permanent, simulated-1g-environment space station in Martian orbit. The focus of the project is to create a temporary, but long-term living environment that focuses on human health and food production. The project is composed of four major habitable parts (in addition to other major components). Moving from the center out, the first is a central core that acts as a hub that connects to the structural spokes, the main propulsion rockets and serves as a place for other spacecraft to dock onto, in addition to housing some of the basic station control, navigation and automated computer systems. The second set of parts are the spokes that connect the ring to the central core; these are the primary structural components with elevators in the middle carrying people from the ring to the core. The ‘lower’ portions, near the ring and it’s 1g environment, also act as storage centers. The third part is the 2-story outer ring This project served as an engineering senior design project at Temple University, and as part of NASA’s eXploration and Habitation (X-Hab) 2018 Academic Innovation Challenge. The goal of the project was to design, construct, and test a device which would be used in microgravity to sanitize produce. This is part of a NASA’s effort to grow and sanitize fresh produce on the International Space Station (ISS), in order to provide more nutritious meal options to the astronauts. Our team designed a 3-D printed spherical device with UV-C LEDs lining the interior. The designed device consists of an 8” spherical chamber, 10 UV-C LEDs, and a touchscreen user interface to control the device. Our design meets the project requirements by offering several features: a simple user interface, minimal crew time to operate, no waste produced, and the ability to sanitize multiple types of produce. The main reductions in Salmonella bacteria and Aspergillus Flavus mold were not verified. However, the aerobic plate count requirement was greatly exceeded and validated. Testing of the device resulted in visual results confirming significant decrease in E. coli activity with increased UV-C exposure, as well as quantitative results confirming significant reduction in CFU/g for the aerobic plate count. These reductions achieved from 1.464 x 10 7 to 2 x 10 4 CFU/g (2.86 log reduction) after 5 minutes, were significantly more than the requirements given by NASA ( of the craft, which holds all of the food production along with medical bays, a control center and a few parks. This ring also integrates the rigid keystone modules, which are the interface between the spokes, the expandable-construction portions of the ring and the habitation pods. The keystone modules also incorporate redundant, distributed ECLSS systems, medical storage, airlocks, and other integral station functions. The last parts are the habitation pods; these hold the sleeping quarters along with the bathrooms, showers, kitchen space and lounge areas. These expandable modules latch onto the keystone modules of the ring, which serves as a circulatory link between the different habitation pods.

Damien Gordon↗

Preliminary structural design conceptualization for composite rotor for verdant power water current turbine

Sandia National Laboratories (SNL) and Verdant Power Inc. (VPI) have partnered under a Cooperative Research and Development Agreement (CRADA) to develop a new kinetic hydropower rotor. The rotor features an improved hydrodynamic and structural design which features state-of-the-art technology developed for the wind industry. The new rotor will have higher energy capture, increased system reliability, and reduction of overall cost of energy. This project was divided into six tasks: (1) Composite Rotor Project Planning and Design Specification; (2) Baseline Fatigue Testing and Failure analysis; (3) Develop Blade/Rotor Performance Model; (4) Hydrofoil Survey and Selection; (5) FEM Structural Design; and (6) Develop Candidate Rotor Designs and Prepare Final Report.

13 HYDRO ENERGY↗

The impact of crystal symmetry on the electronic structure and functional properties of complex lanthanum chromium oxides

Complex oxides exhibit a wide range of crystal structures, chemical compositions and physical properties. The underlying drivers determining the complicated structure-composition-property phase diagrams are the relative positions and orbital overlaps between the metal cations and the oxygen anions. Here we report a combined experimental and theoretical investigation of the structure and bonding in a series of lanthanum chromium oxides prepared by reactive molecular beam epitaxy. Of particular interest is the charge state and local coordination of the Cr. We have stabilized LaCrO 3 , LaCrO 4 and La 2 CrO 6 films by controlling the three elemental fluxes during deposition, and have carried out x-ray diffraction, x-ray photoemission, and x-ray absorption spectroscopy, as well as first-principles calculations, to determine structure, charge state, chemical bonding, and electronic structure. Significant changes in bonding character and orbital interaction are revealed with decreasing ligand symmetry from octahedral to tetrahedral Cr coordination. Both LaCrO 4 and LaCrO 6 with tetrahedrally coordinated Cr show strong pre-edge features in the Cr K-edge near-edge structure whereas LaCrO 3 with octahedrally coordinated Cr exhibits very weak pre-edge features. The origin of these pre-edge features is discussed based on various selection rules and ligand symmetry. We also demonstrate an increase in cation-anion orbital hybridization and a decrease in long-range ligand coupling induced by this symmetry reduction. These in turn result in dramatic modifications of the macroscopic optical and magnetic properties.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

A generalized approximate control variate framework for multifidelity uncertainty quantification

We describe and analyze a variance reduction approach for Monte Carlo (MC) sampling that accelerates the estimation of statistics of computationally expensive simulation models using an ensemble of models with lower cost. These lower cost models — which are typically lower fidelity with unknown statistics — are used to reduce the variance in statistical estimators relative to a MC estimator with equivalent cost. We derive the conditions under which our proposed approximate control variate framework recovers existing multifidelity variance reduction schemes as special cases. We demonstrate that existing recursive/nested strategies are suboptimal because they use the additional low-fidelity models only to efficiently estimate the unknown mean of the first low-fidelity model. As a result, they cannot achieve variance reduction beyond that of a control variate estimator that uses a single low-fidelity model with known mean. However, there often exists about an order-of-magnitude gap between the maximum achievable variance reduction using all low-fidelity models and that achieved by a single low-fidelity model with known mean. We show that our proposed approach can exploit this gap to achieve greater variance reduction by using non-recursive sampling schemes. The proposed strategy reduces the total cost of accurately estimating statistics, especially in cases where only low-fidelity simulation models are accessible for additional evaluations. Several analytic examples and an example with a hyperbolic PDE describing elastic wave propagation in heterogeneous media are used to illustrate the main features of the methodology.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Low-Temperature Restructuring of CeO 2 -Supported Ru Nanoparticles Determines Selectivity in CO 2 Catalytic Reduction

CO 2 reduction to higher value products is a promising way to produce fuels and key chemical building blocks while reducing CO 2 emissions. The reaction at atmospheric pressure mainly yields CH 4 via methanation and CO via the reverse water-gas shift (RWGS) reaction. Describing catalyst features that control the selectivity of these two pathways is important to determine the formation of specific products. At the same time, identification of morphological changes occurring to catalysts under reaction conditions can be crucial to tune their catalytic performance. In this contribution we investigate the dependency of selectivity for CO 2 reduction on the size of Ru nanoparticles (NPs) and on support. We find that even at rather low temperatures (210 °C), oxidative pretreatment induces redispersion of Ru NPs supported on CeO 2 and leads to a complete switch in the performance of this material from a well-known selective methanation catalyst to an active and selective RWGS catalyst. By utilizing in situ X-ray absorption spectroscopy, we demonstrate that the low-temperature redispersion process occurs via decomposition of the metal oxide phase with size-dependent kinetics, producing stable single-site RuO x /CeO 2 species strongly bound to the CeO 2 support that are remarkably selective for CO production. Furthermore, these results show that reaction selectivity can be heavily dependent on catalyst structure and that structural changes of the catalyst can occur even at low temperatures and can go unseen in materials with less defined structures.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Reduction in anti-ferromagnetic interactions in ion-beam deposited Fe{sub 3}O{sub 4} thin films

Phase pure Fe{sub 3}O{sub 4} thin films of thickness {approx}42 nm have been prepared on the Si(100) substrate by reactive ion beam sputtering in the growth temperature range of 150-250 deg. C. A high degree of phase purity in the 175 deg. C sample has been confirmed by the XRD, Raman shift, and R-T measurements. The polycrystalline films show a sharp Verway transition as supported by temperature dependent resistivity, AC susceptibility, and coercivity behavior. The significant feature of these films is the early saturation of their room temperature magnetization at {approx}400 mT, indicating the presence of low anti-ferromagnetic competitions in sharp contrast to most of the previous reports. The noticeable reduction of anti-phase boundaries and its dependence on growth temperature has been correlated with the energetic ion-beam deposition process, and explained in terms of the of ionic vacancy migration approach of Eerenstein et al.[Phys. Rev. B 68, 014428 (2003)]. The electronic conduction of these films is governed by near-neighbor hopping above 240 K and Shklovskii-Efros variable range hopping below this transition temperature.

36 MATERIALS SCIENCE↗

A shift between mineral and nonmineral sources of iron and sulfur causes proteome-wide changes in Methanosarcina barkeri

ABSTRACT Iron (Fe) and sulfur (S) are required elements for life, and changes in their availability can limit the ecological distribution and function of microorganisms. In anoxic environments, soluble Fe typically exists as ferrous iron [Fe(II)] and S as sulfide (HS − ). These species exhibit a strong affinity that ultimately drives the formation of sedimentary pyrite (FeS 2 ). Recently, paradigm-shifting studies indicate that Fe and S in FeS 2 can be made bioavailable by methanogens through a reductive dissolution process. However, the impact of the utilization of FeS 2 , as opposed to canonical Fe and S sources, on the phenotype of cells is not fully understood. Here, shotgun proteomics was utilized to measure changes in the phenotype of Methanosarcina barkeri MS grown with FeS 2 , Fe(II)/HS − , or Fe(II)/cysteine. Shotgun proteomics tracked 1,019 proteins overall, with 307 observed to change between growth conditions. Functional characterization and pathway analyses revealed these changes to be systemic and largely tangential to Fe/S metabolism. As a final step, the proteomics data were viewed with respect to previously collected transcriptomics data to deepen the analysis. Presented here is evidence that M. barkeri adopts distinct phenotypes to exploit specific sources of Fe and S in its environment. This is supported by observed protein abundance changes across broad categories of cellular biology. DNA adjacent metabolism, central carbon metabolism methanogenesis, metal trafficking, quorum sensing, and porphyrin biosynthesis pathways are all features in the phenotypic differentiation. Differences in trace metal availability attributed to complexation with HS − , either as a component of the growth medium [Fe(II)/HS − ] or generated through reduction of FeS 2 , were likely a major factor underpinning these phenotypic differences. IMPORTANCE The methanogenic archaeon Methanosarcina barkeri holds great potential for industrial bio-mining and energy generation technologies. Much of the biochemistry of this microbe is poorly understood, and its characterization will provide a glimpse into biological processes that evolved close to life’s origin. The discovery of its ability to extract iron and sulfur from bulk, solid-phase minerals shifted a longstanding paradigm that these elements were inaccessible to biological systems. The full elucidation of this process has the potential to help scientists and engineers extract valuable metals from low-grade ore and mine waste generating energy in the form of methane while doing so.

59 BASIC BIOLOGICAL SCIENCES↗

Optimizing tuning masses for helicopter rotor blade vibration reduction including computed airloads and comparison with test data

The development and validation of an optimization procedure to systematically place tuning masses along a rotor blade span to minimize vibratory loads are described. The masses and their corresponding locations are the design variables that are manipulated to reduce the harmonics of hub shear for a four-bladed rotor system without adding a large mass penalty. The procedure incorporates a comprehensive helicopter analysis to calculate the airloads. Predicting changes in airloads due to changes in design variables is an important feature of this research. The procedure was applied to a one-sixth, Mach-scaled rotor blade model to place three masses and then again to place six masses. In both cases the added mass was able to achieve significant reductions in the hub shear. In addition, the procedure was applied to place a single mass of fixed value on a blade model to reduce the hub shear for three flight conditions. The analytical results were compared to experimental data from a wind tunnel test performed in the Langley Transonic Dynamics Tunnel. The correlation of the mass location was good and the trend of the mass location with respect to flight speed was predicted fairly well. However, it was noted that the analysis was not entirely successful at predicting the absolute magnitudes of the fixed system loads.

Pritchard, Jocelyn I.↗

On the Microstructure and Solidification Behavior of N-Bearing Ti-46Al-8Ta (at.%) Intermetallic Alloys

Ti-46Al-8Ta (at.%) intermetallic alloys exhibit acceptable strength at low and high temperatures with good oxidation resistance. Different alloying elements are usually added to TiAl-based alloys to develop required properties. In this work, effects of N addition up to 2 at.% on microstructural features and solidification behavior of the Ti-46Al-8Ta alloy is investigated. The achieved results showed that alloying with N causes dendrite refinement and reduction of the retained metastable B2 phase in the as-cast microstructure. The dendrite morphology is also changed from fourfold to fivefold and then sixfold symmetry by increasing N content. The effect of N on interlamellar spacing, amount of constituent phases, and lattice parameters was not uniform. Increasing N content resulted in an increase in both small punch maximum load and hardness up to 1.05 at.%, whereas further N alloying, despite increasing of hardness, led to a drop in the maximum load.

36 MATERIALS SCIENCE↗

Closely spaced nanomagnets by dual e-beam exposure for low-energy nanomagnet logic

The effect of nanomagnet spacing on required clock field has been studied by micromagnetic simulation for supermalloy (Ni{sub 79}Fe{sub 16}Mo{sub 5}) dots with dimensions 90 Multiplication-Sign 60 Multiplication-Sign 20 nm{sup 3} and 120 Multiplication-Sign 60 Multiplication-Sign 20 nm{sup 3}. Reduction of the inter-magnet spacing for both dimensions has resulted in reduction of the required clock field in the simulation. A dual e-beam exposure technique has been developed to allow fabrication of ultra dense features using conventional poly(methylmethacrylate) e-beam resist. Nanomagnet logic (NML) datalines of supermalloy dots with {approx}10 nm and {approx}15 nm spacing have been fabricated using dual e-beam exposure with a 3{sigma} overlay accuracy of {approx}4 nm. Fabricated NML datalines have been characterized using magnetic force microscopy for various clock fields. Datalines of both spacing have shown proper NML functionality with a clock field as low as 60 mT.

36 MATERIALS SCIENCE↗

Model fusion with physics-guided machine learning: Projection-based reduced-order modeling

The unprecedented amount of data generated from experiments, field observations, and large-scale numerical simulations at a wide range of spatiotemporal scales has enabled the rapid advancement of data-driven and especially deep learning models in the field of fluid mechanics. Although these methods are proven successful for many applications, there is a grand challenge of improving their generalizability. This is particularly essential when data-driven models are employed within outer-loop applications like optimization. In this work, we put forth a physics-guided machine learning (PGML) framework that leverages the interpretable physics-based model with a deep learning model. Leveraging a concatenated neural network design from multi-modal data sources, the PGML framework is capable of enhancing the generalizability of data-driven models and effectively protects against or inform about the inaccurate predictions resulting from extrapolation. We apply the PGML framework as a novel model fusion approach combining the physics-based Galerkin projection model and long- to short-term memory (LSTM) network for parametric model order reduction of fluid flows. We demonstrate the improved generalizability of the PGML framework against a purely data-driven approach through the injection of physics features into intermediate LSTM layers. Our quantitative analysis shows that the overall model uncertainty can be reduced through the PGML approach, especially for test data coming from a distribution different than the training data. Moreover, we demonstrate that our approach can be used as an inverse diagnostic tool providing a confidence score associated with models and observations. The proposed framework also allows for multi-fidelity computing by making use of low-fidelity models in the online deployment of quantified data-driven models.

42 ENGINEERING↗

The interplay between laser focusing conditions, expansion dynamics, ablation mechanisms, and emission intensity in ultrafast laser-produced plasmas

The interplay between ultrafast laser focusing conditions, emission intensity, expansion dynamics, and ablation mechanisms is critical to the detection of light isotopes relevant to nuclear energy, forensics, and geochemistry applications. Here, we study deuterium (2Hα) emission in plasmas generated from femtosecond laser ablation of a Zircaloy-4 target with a deuterium concentration of ≈37 at. %. Changes in emission intensity, plume morphology, crater dimensions, and surface modifications were investigated for varying focusing lens positions, where the laser was focused behind, at, and in front of the target. Spatially resolved optical emission spectroscopy and spectrally integrated plasma imaging were performed to investigate emission spectral features and plume morphology. Laser ablation crater dimensions and morphology were analyzed via optical profilometry and scanning electron microscopy. The 2Hα emission intensity showed significant reduction at the geometrical focal point or when the focal point is in front of the target. For all laser spot sizes, a two-component plume was observed but with different temporal histories. At the best focal point, the plume was spherical. When the laser was focused behind the target, the plume was elongated and propagated to farther distances than for the best focal position. In contrast, when the laser was focused in front of the target, filaments were generated in the beam path, and filament-plasma coupling occurred. By focusing the laser behind the target, the amount of material removal in the laser ablation process can be significantly reduced while still generating a plasma with a sufficient 2Hα emission signal for analysis.

Kautz, Elizabeth J. (ORCID:0000000263389223)↗

DeconTools

DeconTools is a software package used for detecting features in MS data by using the isotopic signatures of expected components. The tool uses different algorithms for different parts of the deconvolution process: noise reduction, peak detection, prediction of theoretical isotopic envelope and scoring functions that quantitate the quality of signature observed in the data.

Crowell, Kevin↗

Report on the FY18 Uniaxial Material Model Testing and Key Feature Test Articles Testing of Grade 91

This report summarizes the results from experiments performed in FY18 on Gr. 91 steel (heat 30176). The testing of Gr. 91 has technical significance because, currently, it is the only approved material for Class A construction in ASME Section III Division 5 that is strongly cyclic softening. The focus of this investigation is the effect of pre-cyclic loading history on the responses of Gr. 91 under key feature testing with Simplified Model Test (SMT) creep fatigue and two-bar thermal ratchet loading. For the pre-softened SMT, the SMT life reduction ratio was found to be approximately the same as the pre-softening fatigue damage ratio. The pre-softening also affected the ratchet behavior for the SMT specimens. Under the same two-bar thermal ratchet test conditions, the pre-softened specimens showed faster ratchet ting rates or larger accumulated strains than those without pre-softening history. In addition, results from previous uniaxial mechanical tests in support of the viscoplastic material model development for Gr. 91 are summarized, and the new results from thermomechanical testing at high temperature range are reported.

36 MATERIALS SCIENCE↗

Towards Reconstruction of Complex Flow Fields Using Unit Flows

Many complex turbulent flows in nature and engineering can be qualitatively regarded as being constituted of multiple simpler unit flows. The objective of this work is to characterize the coherent structures in such complex flows as a combination of constituent unitary flow structures for the purpose of reduced-order representation. While turbulence is clearly a non-linear phenomenon, we aim to establish the degree to which the optimally weighted superposition of unitary flow structures can represent the complex flow structures. The rationale for investigating such superposition stems from the fact that the large-scale coherent structures are generated by underlying flow instabilities that may be reasonably described using linear analysis. Clearly, the degree of validity of superposition will depend on the flow under consideration. In this work, we take the first step toward establishing a procedure for investigating superposition. Experimental data of single and triple tandem jets in crossflow are used to demonstrate the procedure. A composite triple tandem jet flow field is generated from optimal superposition of single jet data and compared against ‘true’ triple jet data. Direct comparisons between the true and composite fields are made for spatial, temporal, and kinetic energy content. The large-scale features (obtained from proper orthogonal decomposition or POD) of true and composite tandem jet wakes exhibit nearly 70% agreement in terms of modal eigenvector correlation. Corresponding eigenvalues reveal that the kinetic energy of the flow is also emulated with only a slight overprediction. Temporal frequency features are also examined in an effort to completely characterize POD modes. The proposed method serves as a foundation for more rigorous and robust dimensional reduction in complex flows based on unit flow modes.

Kristo, Paul J.↗