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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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DOE OSTI2012

RECONCILING THE EMISSION MECHANISM DISCREPANCY IN MIRA'S TAIL AND ITS EVOLUTION IN AN INTERFACE WITH SHEAR

Galaxy Evolution Explorer observations of the Mira AB binary system revealed a surrounding structure that has been successfully hydrodynamically interpreted as a bow shock and tail of ram-pressure-stripped material. Even the narrow tail, initially difficult to model, has been understood to be the effect of the passage of Mira from a warm neutral medium into a hot, low-density medium, postulated to be the Local Bubble. However, no model to date has explained the observed kink and associated general curvature of the tail. We test the hypothesis that before entering the Local Bubble, Mira was traveling through a shear flow with approximately 1/3 Mira's own velocity at an angle of 30 Degree-Sign to Mira's proper motion. The hypothesis reproduces the kinked nature of Mira's tail and predicts recompression and reheating of the tail material to the same or greater levels of density and temperature predicted in the shock. This provides a heat source for the FUV emission, allowing for an extended lifetime of the FUV emission in line with other estimates of the age of the tail. The uniqueness of Mira's situation implies that the chances of observing other FUV tails behind asymptotic giant branch stars are highly unlikely.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2012

LOWER-LUMINOSITY GALAXIES COULD REIONIZE THE UNIVERSE: VERY STEEP FAINT-END SLOPES TO THE UV LUMINOSITY FUNCTIONS AT z {>=} 5-8 FROM THE HUDF09 WFC3/IR OBSERVATIONS

The HUDF09 data are the deepest near-IR observations ever, reaching to 29.5 mag. Luminosity functions (LFs) from these new HUDF09 data for 132 z {approx} 7 and z {approx} 8 galaxies are combined with new LFs for z {approx} 5-6 galaxies and the earlier z {approx} 4 LF to reach to very faint limits (<0.05 L*{sub z=3}). The faint-end slopes {alpha} are steep: -1.79 {+-} 0.12 (z {approx} 5), -1.73 {+-} 0.20 (z {approx} 6), -2.01 {+-} 0.21 (z {approx} 7), and -1.91 {+-} 0.32 (z {approx} 8). Slopes {alpha} {approx}< -2 lead to formally divergent UV fluxes, though galaxies are not expected to form below {approx} - 10 AB mag. These results have important implications for reionization. The weighted mean slope at z {approx} 6-8 is -1.87 {+-} 0.13. For such steep slopes, and a faint-end limit of -10 AB mag, galaxies provide a very large UV ionizing photon flux. While current results show that galaxies can reionize the universe by z {approx} 6, matching the Thomson optical depths is more challenging. Extrapolating the current LF evolution to z > 8, taking {alpha} to be -1.87 {+-} 0.13 (the mean value at z {approx} 6-8), and adopting typical parameters, we derive Thomson optical depths of 0.061{sup +0.009}{sub -0.006}. However, this result will change if the faint-end slope {alpha} is not constant with redshift. We test this hypothesis and find a weak, though uncertain, trend to steeper slopes at earlier times (d{alpha}/dz {approx} -0.05 {+-} 0.04) that would increase the Thomson optical depths to 0.079{sup +0.063}{sub -0.017}, consistent with recent WMAP estimates ({tau} = 0.088 {+-} 0.015). It may thus not be necessary to resort to extreme assumptions about the escape fraction or clumping factor. Nevertheless, the uncertainties remain large. Deeper WFC3/IR+ACS observations can further constrain the UV ionizing flux from faint galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2012

SATELLITES AROUND MASSIVE GALAXIES SINCE z {approx} 2: CONFRONTING THE MILLENNIUM SIMULATION WITH OBSERVATIONS

Minor merging has been postulated as the most likely evolutionary path to produce the increase in size and mass observed in the massive galaxies since z {approx} 2. In this Letter, we directly test this hypothesis, comparing the population of satellites around massive galaxies in cosmological simulations versus the observations. We use state-of-the-art, publically available, Millennium I and II simulations, and the associated semi-analytical galaxy catalogs to explore the time evolution of the fraction of massive galaxies that have satellites, the number of satellites per galaxy, the projected distance at which the satellites locate from the host galaxy, and the mass ratio between the host galaxies and their satellites. The three virtual galaxy catalogs considered here overproduce the fraction of galaxies with satellites by a factor ranging between 1.5 and 6 depending on the epoch, whereas the mean projected distance and ratio of the satellite mass over host mass are in closer agreement with data. The larger pull of satellites in the semi-analytical samples could suggest that the size evolution found in previous hydrodynamical simulations is an artifact due to the larger number of infalling satellites compared to the real universe. These results advise us to revise the physical ingredients implemented in the semi-analytical models in order to reconcile the observed and computed fraction of galaxies with satellites, and eventually, it would leave some room for other mechanisms explaining the galaxy size growth not related to the minor merging.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2013

STELLAR MASS VERSUS STELLAR VELOCITY DISPERSION: WHICH IS BETTER FOR LINKING GALAXIES TO THEIR DARK MATTER HALOS?

It was recently suggested that compared to its stellar mass (M{sub *}), the central stellar velocity dispersion ({sigma}{sub *}) of a galaxy might be a better indicator for its host dark matter halo mass. Here we test this hypothesis by estimating the dark matter halo mass for central galaxies in groups as a function of M{sub *} and {sigma}{sub *}. For this we have estimated the redshift-space cross-correlation function (CCF) between the central galaxies at given M{sub *} and {sigma}{sub *} and a reference galaxy sample, from which we determine both the projected CCF, w{sub p} (r{sub p} ), and the velocity dispersion profile. A halo mass is then obtained from the average velocity dispersion within the virial radius. At fixed M{sub *}, we find very weak or no correlation between halo mass and {sigma}{sub *}. In contrast, strong mass dependence is clearly seen even when {sigma}{sub *} is limited to a narrow range. Our results thus firmly demonstrate that the stellar mass of central galaxies is still a good (if not the best) indicator for dark matter halo mass, better than the stellar velocity dispersion. The dependence of galaxy clustering on {sigma}{sub *} at fixed M{sub *}, as recently discovered by Wake et al., may be attributed to satellite galaxies, for which the tidal stripping occurring within halos has stronger effect on stellar mass than on central stellar velocity dispersion.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2013

A SUBSTANTIAL MASS OF COOL, METAL-ENRICHED GAS SURROUNDING THE PROGENITORS OF MODERN-DAY ELLIPTICALS

The hosts of luminous z {approx} 2 quasars evolve into today's massive elliptical galaxies. Current theories predict that the circumgalactic medium (CGM) of these massive, dark matter halos (M{sub DM} {approx} 10{sup 12.5} M{sub Sun }) should be dominated by a T {approx} 10{sup 7} K virialized plasma. We test this hypothesis with observations of 74 close-projected quasar pairs, using spectra of the background QSO to characterize the CGM of the foreground one. Surprisingly, our measurements reveal a cool (T Almost-Equal-To 10{sup 4} K), massive (M{sub CGM} > 10{sup 10} M{sub Sun }), and metal-enriched (Z {approx}> 0.1 Z{sub Sun }) medium extending to at least the expected virial radius (r{sub vir} = 160 kpc). The average equivalent widths of H I Ly{alpha} (W-bar{sub Ly{alpha}}= 2.1{+-}0.15 A for impact parameters R < 200 kpc) and C II 1334 (W-bar{sub 1334}= 0.7{+-}0.1) exceed the corresponding CGM measurements of these transitions from all galaxy populations studied previously. Furthermore, we conservatively estimate that the quasar CGM has a 64{sup +6}{sub -7}% covering fraction of optically thick gas (N{sub HI} > 10{sup 17.2} cm{sup -2}) within r{sub vir}; this covering factor is twice that of the contemporaneous Lyman break galaxy population. This unexpected reservoir of cool gas is rarely detected 'down-the-barrel' to quasars, and hence it is likely that our background sight lines intercept gas that is shadowed from the quasar ionizing radiation by the same obscuring medium often invoked in models of active galactic nucleus unification. Because the high-z halos inhabited by quasars predate modern groups and clusters, these observations are also relevant to the formation and enrichment history of the intragroup/intracluster medium.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2013

OBSERVATION OF HEATING BY FLARE-ACCELERATED ELECTRONS IN A SOLAR CORONAL MASS EJECTION

We report a Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) observation of flare-accelerated electrons in the core of a coronal mass ejection (CME) and examine their role in heating the CME. Previous CME observations have revealed remarkably high thermal energies that can far surpass the CME's kinetic energy. A joint observation by RHESSI and the Atmospheric Imaging Assembly of a partly occulted flare on 2010 November 3 allows us to test the hypothesis that this excess energy is collisionally deposited by flare-accelerated electrons. Extreme ultraviolet (EUV) images show an ejection forming the CME core and sheath, with isothermal multifilter analysis revealing temperatures of ∼11 MK in the core. RHESSI images reveal a large (∼100 × 50 arcsec{sup 2}) hard X-ray (HXR) source matching the location, shape, and evolution of the EUV plasma, indicating that the emerging CME is filled with flare-accelerated electrons. The time derivative of the EUV emission matches the HXR light curve (similar to the Neupert effect observed in soft and HXR time profiles), directly linking the CME temperature increase with the nonthermal electron energy loss, while HXR spectroscopy demonstrates that the nonthermal electrons contain enough energy to heat the CME. This is the most direct observation to date of flare-accelerated electrons heating a CME, emphasizing the close relationship of the two in solar eruptive events.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2015

A FAST RADIO BURST IN THE DIRECTION OF THE CARINA DWARF SPHEROIDAL GALAXY

We report the real-time discovery of a fast radio burst (FRB 131104) with the Parkes radio telescope in a targeted observation of the Carina dwarf spheroidal galaxy. The dispersion measure of the burst is 779 cm{sup –3} pc, exceeding predictions for the maximum line-of-sight Galactic contribution by a factor of 11. The temporal structure of the burst is characterized by an exponential scattering tail with a timescale of 2.0{sub −0.5}{sup +0.8} ms at 1582 MHz that scales as frequency to the power –4.4{sub −1.8}{sup +1.6} (all uncertainties represent 95% confidence intervals). We bound the intrinsic pulse width to be <0.64 ms due to dispersion smearing across a single spectrometer channel. Searches in 78 hr of follow-up observations with the Parkes telescope reveal no additional sporadic emission and no evidence for associated periodic radio emission. We hypothesize that the burst is associated with the Carina dwarf galaxy. Follow-up observations at other wavelengths are necessary to test this hypothesis.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2017

Force-induced transcellular tunnel formation in endothelial cells

The endothelium serves as a protective semipermeable barrier in blood vessels and lymphatic vessels. Leukocytes and pathogens can pass directly through the endothelium by opening holes in endothelial cells, known as transcellular tunnels, which are formed by contact and self-fusion of the apical and basal plasma membranes. Here we test the hypothesis that the actin cytoskeleton is the primary barrier to transcellular tunnel formation using a combination of atomic force microscopy and fluorescence microscopy of live cells. We find that localized mechanical forces are sufficient to induce the formation of transcellular tunnels in human umbilical vein endothelial cells (HUVECs). When HUVECs are exposed to the bacterial toxin called epidermal cell differentiation inhibitor (EDIN), which can induce spontaneous transcellular tunnels, less mechanical work is required to form tunnels due to the reduced cytoskeletal stiffness and thickness of these cells, similarly to the effects of a Rho-associated protein kinase (ROCK) inhibitor. We also observe actin enrichment in response to mechanical indentation that is reduced in cells exposed to the bacterial toxin. Our study shows that the actin cytoskeleton of endothelial cells provides both passive and active resistance against transcellular tunnel formation, serving as a mechanical barrier that can be overcome by mechanical force as well as disruption of the cytoskeleton.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

The starch-deficient plastidic PHOSPHOGLUCOMUTASE mutant of the constitutive crassulacean acid metabolism (CAM) species Kalanchoë fedtschenkoi impacts diel regulation and timing of stomatal CO2 responsiveness

Abstract Background and Aims Crassulacean acid metabolism (CAM) is a specialized type of photosynthesis characterized by a diel pattern of stomatal opening at night and closure during the day, which increases water-use efficiency. Starch degradation is a key regulator of CAM, providing phosphoenolpyruvate as a substrate in the mesophyll for nocturnal assimilation of CO2. Growing recognition of a key role for starch degradation in C3 photosynthesis guard cells for mediating daytime stomatal opening presents the possibility that starch degradation might also impact CAM by regulating the provision of energy and osmolytes to increase guard cell turgor and drive stomatal opening at night. In this study, we tested the hypothesis that the timing of diel starch turnover in CAM guard cells has been reprogrammed during evolution to enable nocturnal stomatal opening and daytime closure. Methods Biochemical and genetic characterization of wild-type and starch-deficient RNAi lines of Kalanchoë fedtschenkoi with reduced activity of plastidic phosphoglucomutase (PGM) constituted a preliminary approach for the understanding of starch metabolism and its implications for stomatal regulation in CAM plants. Key Results Starch deficiency reduced nocturnal net CO2 uptake but had negligible impact on nocturnal stomatal opening. In contrast, daytime stomatal closure was reduced in magnitude and duration in the starch-deficient rPGM RNAi lines, and their stomata were unable to remain closed in response to elevated concentrations of atmospheric CO2 administered during the day. Curtailed daytime stomatal closure was linked to higher soluble sugar contents in the epidermis and mesophyll. Conclusions Nocturnal stomatal opening is not reliant upon starch degradation, but starch biosynthesis is an important sink for carbohydrates, ensuring daytime stomatal closure in this CAM species.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

A mixture of grass–legume cover crop species may ameliorate water stress in a changing climate

Climate change models predict increasing precipitation variability in the mid-latitude regions of Earth, generating a need to reduce the negative impacts of these changes on crop production. Despite considerable research on how cover crops support agriculture in a changing climate, understanding is limited of how climate change influences the growth of cover crops. We investigated the early development of two common cover crop species—crimson clover (Trifolium incarnatum) and rye (Secale cereale)—and hypothesized that growing them in the mixture would ameliorate stress from drought or waterlogging. This hypothesis was tested in a 25-day greenhouse experiment, where the two factors (species number and water stress) were fully crossed in randomized blocks, and plant responses were quantified through survival, growth rate, biomass production and root morphology. Water stress negatively influenced the early growth of these two species in contrasting ways: crimson clover was susceptible to drought while rye performed poorly under waterlogging. Per-plant biomass in rye was always greater in mixture than in monoculture, while per-plant biomass of crimson clover was greater in mixture under drought. Both species grew longer roots in mixture than in monoculture under drought, and total biomass of mixtures did not differ significantly from the more-productive monoculture (rye) in any water condition. In the face of increasingly variable precipitation, growing crimson clover and rye together has potential to ameliorate water stress, a possibility that should be further investigated in field experiments.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2018

Dispersal limitation and thermodynamic constraints govern spatial structure of permafrost microbial communities

Understanding drivers of permafrost microbial community composition is critical for understanding permafrost microbiology and predicting ecosystem responses to thaw, however studies describing ecological controls on these communities are lacking. We hypothesize that permafrost communities are uniquely shaped by constraints imposed by prolonged freezing, and decoupled from factors that influence non-permafrost soil communities. To test this hypothesis, we characterized patterns of environmental variation and microbial community composition in permafrost across an Alaskan boreal forest landscape. We used null modeling to estimate the relative importance of selective and neutral assembly processes on community composition, and identified environmental factors influencing ecological selection through regression and structural equation modeling (SEM). Proportionally, the strongest process influencing community composition was dispersal limitation (0.36), exceeding the influence of homogenous selection (0.21), variable selection (0.16), and homogenizing dispersal (0.05). Fe(II) content was the most important factor explaining variable selection, and was significantly associated with total selection by univariate regression (R2=0.14, 41 p=0.003). SEM supported a model in which Fe(II) content mediated influences of the Gibbs free energy of the organic matter pool and organic acid concentration on total selection. These findings reveal that the processes shaping microbial communities in permafrost are distinct from those in non-permafrost soils, as the stability of the permafrost environment imposes dispersal and thermodynamic constraints on permafrost communities. Models of permafrost community composition will need to account for these unique drivers in order to predict community characteristics across permafrost landscapes, and in efforts to understand how pre-thaw conditions will influence post-thaw ecological and biogeochemical processes.

permafrost↗
DOE OSTI2021

Phosphorolytic degradation of leaf starch via plastidic α-glucan phosphorylase leads to optimized plant growth and water use efficiency over the diel phases of Crassulacean acid metabolism

In plants with Crassulacean acid metabolism (CAM), it has been proposed that the requirement for nocturnal provision of phosphoenolpyruvate as a substrate for CO 2 uptake has resulted in a re-routing of chloroplastic starch degradation from the amylolytic route to the phosphorolytic route. To test this hypothesis, we generated and characterized four independent RNAi lines of the obligate CAM species Kalanchoë fedtschenkoi with a >10-fold reduction in transcript abundance of plastidic α-glucan phosphorylase (PHS1). The rPHS1 lines showed diminished nocturnal starch degradation, reduced dark CO 2 uptake, a reduction in diel water use efficiency (WUE), and an overall reduction in growth. A re-routing of starch degradation via the hydrolytic/amylolytic pathway was indicated by hyperaccumulation of maltose in all rPHS1 lines. Further examination indicated that whilst operation of the core circadian clock was not compromised, plasticity in modulating net dark CO 2 uptake in response to changing photoperiods was curtailed. The data show that phosphorolytic starch degradation is critical for efficient operation of the CAM cycle and for optimizing WUE. This finding has clear relevance for ongoing efforts to engineer CAM into non-CAM species as a means of boosting crop WUE for a warmer, drier future.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Can improved canopy light transmission ameliorate loss of photosynthetic efficiency in the shade? An investigation of natural variation in Sorghum bicolor

Abstract Previous studies have found that maximum quantum yield of CO2 assimilation (Φ CO2,max,app) declines in lower canopies of maize and miscanthus, a maladaptive response to self-shading. These observations were limited to single genotypes, leaving it unclear whether the maladaptive shade response is a general property of this C4 grass tribe, the Andropogoneae. We explored the generality of this maladaptation by testing the hypothesis that erect leaf forms (erectophiles), which allow more light into the lower canopy, suffer less of a decline in photosynthetic efficiency than drooping leaf (planophile) forms. On average, Φ CO2,max,app declined 27% in lower canopy leaves across 35 accessions, but the decline was over twice as great in planophiles than in erectophiles. The loss of photosynthetic efficiency involved a decoupling between electron transport and assimilation. This was not associated with increased bundle sheath leakage, based on 13C measurements. In both planophiles and erectophiles, shaded leaves had greater leaf absorptivity and lower activities of key C4 enzymes than sun leaves. The erectophile form is considered more productive because it allows a more effective distribution of light through the canopy to support photosynthesis. We show that in sorghum, it provides a second benefit, maintenance of higher Φ CO2,max,app to support efficient use of that light resource.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Reducing stomatal density by expression of a synthetic epidermal patterning factor increases leaf intrinsic water use efficiency and reduces plant water use in a C4 crop

Abstract Enhancing crop water use efficiency (WUE) is a key target trait for climatic resilience and expanding cultivation on marginal lands. Engineering lower stomatal density to reduce stomatal conductance (gs) has improved WUE in multiple C3 crop species. However, reducing gs in C3 species often reduces photosynthetic carbon gain. A different response is expected in C4 plants because they possess specialized anatomy and biochemistry which concentrates CO2 at the site of fixation. This modifies the relationship of photosynthesis (AN) with intracellular CO2 concentration (ci), such that photosynthesis is CO2 saturated and reductions in gs are unlikely to limit AN. To test this hypothesis, genetic strategies were investigated to reduce stomatal density in the C4 crop sorghum. Constitutive expression of a synthetic epidermal patterning factor (EPF) transgenic allele in sorghum led to reduced stomatal densities, reduced gs, reduced plant water use, and avoidance of stress during a period of water deprivation. In addition, moderate reduction in stomatal density did not increase stomatal limitation to AN. However, these positive outcomes were associated with negative pleiotropic effects on reproductive development and photosynthetic capacity. Avoiding pleiotropy by targeting expression of the transgene to specific tissues could provide a pathway to improved agronomic outcomes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2014

Light quality modulates metabolic synchronization over the diel phases of crassulacean acid metabolism

Temporal compartmentation of carboxylation processes is a defining feature of crassulacean acid metabolism and involves circadian control of key metabolic and transport steps that regulate the supply and demand for carbon over a 24h cycle. Recent insights on the molecular workings of the circadian clock and its connection with environmental inputs raise new questions on the importance of light quality and, by analogy, certain photoreceptors for synchronizing the metabolic components of CAM. The present work tested the hypothesis that optimal coupling of stomatal conductance, net CO2 uptake, and the reciprocal turnover of carbohydrates and organic acids over the diel CAM cycle requires both blue and red light input signals. Contrasting monochromatic wavelengths of blue, green, and red light (i.e. 475, 530, 630nm) with low fluence rates (10 μmol m–2 s–1) were administered for 16 hours each diel cycle for a total treatment time of 48 hours to the obligate CAM bromeliad, Aechmea ‘Maya’. Of the light treatments imposed, low-fluence blue light was a key determinant in regulating stomatal responses, organic acid mobilization from the vacuole, and daytime decarboxylation. However, the reciprocal relationship between starch and organic acid turnover that is typical for CAM was uncoupled under low-fluence blue light. Under low-fluence red or green light, the diel turnover of storage carbohydrates was orchestrated in line with the requirements of CAM, but a consistent delay in acid consumption at dawn compared with plants under white or low-fluence blue light was noted. Consistent with the acknowledged influences of both red and blue light as input signals for the circadian clock, the data stress the importance of both red and blue-light signalling pathways for synchronizing the metabolic and physiological components of CAM over the day/night cycle.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2022

The merger fraction of post-starburst galaxies in UNIONS

ABSTRACT Post-starburst galaxies (PSBs) are defined as having experienced a recent burst of star formation, followed by a prompt truncation in further activity. Identifying the mechanism(s) causing a galaxy to experience a post-starburst phase therefore provides integral insight into the causes of rapid quenching. Galaxy mergers have long been proposed as a possible post-starburst trigger. Effectively testing this hypothesis requires a large spectroscopic galaxy survey to identify the rare PSBs as well as high-quality imaging and robust morphology metrics to identify mergers. We bring together these critical elements by selecting PSBs from the overlap of the Sloan Digital Sky Survey and the Canada–France Imaging Survey and applying a suite of classification methods: non-parametric morphology metrics such as asymmetry and Gini-M20, a convolutional neural network trained to identify post-merger galaxies, and visual classification. This work is therefore the largest and most comprehensive assessment of the merger fraction of PSBs to date. We find that the merger fraction of PSBs ranges from 19 per cent to 42 per cent depending on the merger identification method and details of the PSB sample selection. These merger fractions represent an excess of 3–46× relative to non-PSB control samples. Our results demonstrate that mergers play a significant role in generating PSBs, but that other mechanisms are also required. However, applying our merger identification metrics to known post-mergers in the IllustrisTNG simulation shows that 70 per cent of recent post-mergers (≲200 Myr) would not be detected. Thus, we cannot exclude the possibility that nearly all PSBs have undergone a merger in their recent past.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2015

DNA damage in cells exhibiting radiation-induced genomic instability

Cells exhibiting radiation induced genomic instability exhibit varied spectra of genetic and chromosomal aberrations. Even so, oxidative stress remains a common theme in the initiation and/or perpetuation of this phenomenon. Isolated oxidatively modified bases, abasic sites, DNA single strand breaks and clustered DNA damage are induced in normal mammalian cultured cells and tissues due to endogenous reactive oxygen species generated during normal cellular metabolism in an aerobic environment. While sparse DNA damage may be easily repaired, clustered DNA damage may lead to persistent cytotoxic or mutagenic events that can lead to genomic instability. In this study, we tested the hypothesis that DNA damage signatures characterised by altered levels of endogenous, potentially mutagenic, types of DNA damage and chromosomal breakage are related to radiation-induced genomic instability and persistent oxidative stress phenotypes observed in the chromosomally unstable progeny of irradiated cells. The measurement of oxypurine, oxypyrimidine and abasic site endogenous DNA damage showed differences in non-double-strand breaks (DSB) clusters among the three of the four unstable clones evaluated as compared to genomically stable clones and the parental cell line. These three unstable clones also had increased levels of DSB clusters. The results of this study demonstrate that each unstable cell line has a unique spectrum of persistent damage and lead us to speculate that alterations in DNA damage signaling and repair may be related to the perpetuation of genomic instability.

59 BASIC BIOLOGICAL SCIENCES↗