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At least 847 records · Page 47

Two techniques for eliminating luminol interference material and flow system configurations for luminol and firefly luciferase systems

Two methods for eliminating luminol interference materials are described. One method eliminates interference from organic material by pre-reacting a sample with dilute hydrogen peroxide. The reaction rate resolution method for eliminating inorganic forms of interference is also described. The combination of the two methods makes the luminol system more specific for bacteria. Flow system designs for both the firefly luciferase and luminol bacteria detection systems are described. The firefly luciferase flow system incorporating nitric acid extraction and optimal dilutions has a functional sensitivity of 3 x 100,000 E. coli/ml. The luminol flow system incorporates the hydrogen peroxide pretreatment and the reaction rate resolution techniques for eliminating interference. The functional sensitivity of the luminol flow system is 1 x 10,000 E. coli/ml.

Thomas, R. R.↗

Lab-on-a-Chip: From Astrobiology to the International Space Station

The continual and long-term habitation of enclosed environments, such as Antarctic stations, nuclear submarines and space stations, raises unique engineering, medical and operational challenges. There is no easy way out and no easy way to get supplies in. This situation elevates the importance of monitoring technology that can rapidly detect events within the habitat that affect crew safety such as fire, release of toxic chemicals and hazardous microorganisms. Traditional methods to monitor microorganisms on the International Space Station (ISS) have consisted of culturing samples for 3-5 days and eventual sample return to Earth. To augment these culture methods with new, rapid molecular techniques, we developed the Lab-on-a-Chip Application Development - Portable Test System (LOCAD-PTS). The system consists of a hand-held spectrophotometer, a series of interchangeable cartridges and a surface sampling/dilution kit that enables crew to collect samples and detect a range of biological molecules, all within 15 minutes. LOCAD-PTS was launched to the ISS aboard Space Shuttle Discovery in December 2006, where it was operated for the first time during March-May 2007. The surfaces of five separate sites in the US Lab and Node 1 of ISS were analyzed for endotoxin, using cartridges that employ the Limulus Amebocyte Lysate (LAL) assay; results of these tests will be presented. LOCAD-PTS will remain permanently onboard ISS with new cartridges scheduled for launch in February and October of 2008 for the detection of fungi (Beta-glucan) and Gram-positive bacteria (lipoteichoic acid), respectively.

Maule, Jake↗

Reluctant glass formers and their applications in optical lens design

Over ten years ago, it was shown that glasses with high index of refraction and some with low dispersion could be produced from a number of pure refractory oxides, including the lanthanides, by containerless processing. By containerless processing it is possible to minimize surface heterogeneous nucleation and produce larger glass samples of the materials than by other methods. The use of proposed high temperature containerless processing facilities in space permits the fabrication of benchmark samples of new unique glass compositions for optical property determination as well as for glass formation. It was shown that glasses with high refractive indices and large Abbe numbers can be formulated using this technology. These glasses lie in the classical forbidden region of the glass map. Preliminary study of the impact of having such unusual glasses available for the lens designer was made. Results indicate that significant improvements can be realized over the use of only conventional glasses. A cursory survey of a number of nationally recognized lens designers indicated a general agreement that such glasses would be highly desired and could be expected to lead to completely new designs as well as simplifying existing designs.

Ethridge, Edwin C.↗

NASA Li/CF(x) cell problem analysis: Anion exchange chromatography analysis

An analysis was made of wiper samples used to wipe down lithium/chlorine fluorine battery components and production equipment. These components and equipment were potentially exposed to thionyl chloride vapors. In the presence of moisture, thionyl chloride decomposes to sulfur dioxide and hydrogen chloride. The wiper samples were analyzed for soluble chlorides and fluorides by anion exchange chromatography. During the examination of the test chromatographs, fluoride contamination was discovered in wiper samples from the test equipment. An analytical method to determine fluoride was developed. The first 3 extracts from the potentially exposed and clean wiper samples were tested, and the total fluoride from both groups determined. A comparison of the results from both groups was made to determine the extent of fluoride contamination.

Bytella, Joseph↗

Shallow Soil Polychlorinated Biphenyl Site Assessment Report

This report presents a summary of the shallow soil polychlorinated biphenyl (PCB) site assessment activities that occurred from December 2020 through July 2022 at General Services Administration Reclamation Yard, Solid Waste Management Unit (SWMU) 010, located at the John F. Kennedy Space Center (KSC), Florida. The site is monitored under KSC’s Resource Conservation and Recovery Act Corrective Action Program, which also meets the requirements of Chapter 62-780, Florida Administrative Code. For the purposes of this report, three separate shallow soil plumes, known as the Northeast Shallow Soil PCB Plume, Southeast Shallow Soil PCB Plume, and West Shallow Soil PCB Plume, were identified for this site. The activities presented in this report include seven field events conducted between December 2020 and July 2022, which include shallow soil sample collection in the Northeast, Southeast, and West Shallow Soil PCB Plumes. AECOM Technical Services, Inc., personnel collected 330 soil samples from 118 boring locations. The samples were collected at 0.5-foot depth intervals to various depths based on analytical results and depth to groundwater. The soil samples were submitted to a fixed-based laboratory for analysis by United States Environmental Protection Agency Method 8082A for total PCBs. Several soil samples had PCB concentrations above the State of Florida Direct Exposure Residential and Industrial Soil Cleanup Target Levels (SCTLs), and two soil samples had PCB concentrations above the State of Florida Leachability for Groundwater. No soil borings were located beneath impervious surfaces, such as concrete or asphalt. The current Land Use Control Implementation Plan (LUCIP) covers PCBs in soils above the residential SCTL, which includes the paved areas.

PCBs↗

Flap noise prediction method for a powered lift system

A method is presented for estimating the noise generated by deflection of the engine exhaust for under-the-wing and over-the-wing versions of an externally blown flap configuration for powered lift. Correlation equations and curves are given for the overall sound pressure level and directivity and for spectra scaled to a high bypass 25,000-pound thrust size engine. Data are taken from TF34 engine tests and from large cold flow model tests. The correlations are empirical, and thus application of this prediction procedure is limited to geometrically similar configurations. Application of the method is illustrated by calculated sample footprints.

Clark, B.↗

Flap noise prediction method for a powered lift system

A method is presented for estimating the noise generated by deflection of the engine exhaust for under-the-wing and over-the-wing versions of an externally blown flap configuration for powered lift. Correlation equations and curves are given for the OASPL and directivity and for spectra scaled to a high bypass 25,000-pound thrust size engine. Data are taken from TF34 engine tests and from large cold flow model tests. The correlations are empirical, and thus application of this prediction procedure is limited to geometrically similar configurations. Application of the method is illustrated by calculated sample footprints.

Clark, B.↗

Whole-rock uranium analysis by fission track activation

We report a whole-rock uranium method in which the polished sample and track detector are separated in a vacuum chamber. Irradiation with thermal neutrons induces uranium fission in the sample, and the detector records the integrated fission track density. Detection efficiency and geometric factors are calculated and compared with calibration experiments.

Weiss, J. R.↗

Remote monitoring of environmental particulate pollution - A problem in inversion of first-kind integral equations

The determination of the microstructure, chemical nature, and dynamical evolution of scattering particulates in the atmosphere is considered. A description is given of indirect sampling techniques which can circumvent most of the difficulties associated with direct sampling techniques, taking into account methods based on scattering, extinction, and diffraction of an incident light beam. Approaches for reconstructing the particulate size distribution from the direct and the scattered radiation are discussed. A new method is proposed for determining the chemical composition of the particulates and attention is given to the relevance of methods of solution involving first kind Fredholm integral equations.

Fymat, A. L.↗

The earth radiation budget satellite system of the early 1980's

The overall program objective of the Earth Radiation Budget Satellite System is to gather the required radiation budget data and apply these data for a better understanding and prediction of climate. The paper describes the planned system, including the instruments and the associated sampling strategies and data analysis methods. Examination of mission implications reveals the need for a multisensor, multisatellite system consisting of high- and mid-inclination orbits. Each spacecraft will carry wide and medium field-of-view sensors, a sensor for measuring the solar constant, and a narrow field-of-view cross-track scanner.

Cooper, J. E.↗

The application of remote sensing technology to the solution of problems in the management of resources in Indiana

The author has identified the following significant results. Of the sampling techniques considered, a combination soil mapping and area sampling offered the most practical method for gathering soils data. Using the dot grid count, a relative percentage composition of soils can be calculated for each spectral class. From these percentages, a legend describing the dominant soils and inclusions can be developed. Interval drainage class seemed to be correlated with magnitude. For every parent material area, the more poorly drained soils had a lower magnitude of reflectance. Soil spectral classes seemed to be predominantly one internal drainage class.

Weismiller, R. A.↗

Silicon Solar Cell Fabrication Technology

Device fabrication and photoconductive lifetime decay measurements were used to characterize single and polycrystalline silicon substracts. The device characterization of the processed materials was done by spectral response measurements and absolute quantum efficiency at a single wavelength. The results were then reduced to yield the diffusion length of the various samples. The photoconductive lifetime decay method was implemented in order to determine the minority carrier lifetime in unprocessed wafers.

Stafsudd, O. M.↗

Precipitation measurements from space - Workshop summary 28 April-1 May 1981, Greenbelt, MD

The goals and methods for global precipitation measurements from space are examined. Records currently exist only for visible and IR scans of cloud properties, and are applied from GEO for detecting diurnal variations in precipitation. Microwave radiometry is noted to be a suitable method for supplementing the visible and IR data for measuring stratiform oceanic precipitation, and when used at up to 3 microns can detect areas, if not amounts, of precipitation from GEO. Applications of radar altimeters are proposed in terms of modifications to the Seasat-type 2.2 cm radar, the use of surface target attentuation radar, of frequency agile rain radar, or of adaptive pointing radar. Soil moisture sensing is available with passive microwave radiometry in the 20-50 cm bands, or active sensing in the 5-8 cm bands. The utilization of GARP ground truth data is explored, along with statistical methods for treating the data samples.

Atlas, D.↗