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At least 847 records · Page 47

Solar Activity and its Impact on Earth's Climate

The Sun's activity is now approaching an expected 2006 minimum, following the dramatic maximum of Solar Cycle 23, that included events such as the 2001 "Bastille Day" Coronal Mass Ejection, and the record-setting Oct-Nov 2003 solar flares, with their associated sunspots and variations in Total Solar Irradiance, or TSI. On Nov 4,2003 the largest X-ray flare ever detected (X-28) was observed in detail. We discuss recent satellite measurements of TSI by ACRIM 2 and 3 and Virgo, and new precision observations of TSI and SSI (Solar Spectral Irradiance) from the SORCE mission, that launched on January 25,2003. TSI variations recorded during the June 8,2004 transit of Venus show the unprecedented precision of the SORCE Total Irradiance Monitor (TIM) instrument, the first of its kind to employ phase-sensitive detection. The SORCE spectral instruments, XPS, Solstice, and SIM, record the Sun's changes over a wide range of wavelengths, from 1 to more than 2000 nanometers, for the first time covering the peak of the solar spectrum, including spectral components that provide energy inputs to key components of the climate system - ultraviolet (UV) into the upper atmospheric ozone layer, infrared (IR) into the lower atmosphere and clouds, and Visible into the Oceans and biosphere. Succeeding satellite missions are planned to monitor both TSI and SSI through Cycle 24. We summarize current ideas about decadal and longer solar variability, and associated potential impacts on Earth's climate on time scales from decades to centuries, especially highlighting the role of feedbacks in the climate system.

Cahalan, Robert F.↗

Visioning a Global Carbon Monitoring System That Can Quantify and Attribute Ocean Carbon Dioxide Removal: Where Are We Now and How Might We Get There?

As atmospheric greenhouse gas concentrations and the resulting social costs rise, it is increasingly important to conduct research on technical approaches to remove carbon dioxide from the atmosphere at the gigaton scale. One highly uncertain but perhaps plausible solution is ocean carbon dioxide (CO2) removal (CDR), which encompasses a suite of proposed techniques for increasing the net flux of CO2 from the atmosphere to the ocean. As reflected in a 2022 National Academy of Sciences report, interest in ocean CDR is rapidly growing, and new stakeholders from a wide range of fields want access to air-sea CO2 flux and ocean carbon information to understand the potential risks and benefits of ocean CDR. Moreover, there is an urgent need for research to develop a scientific basis to support these societal needs. At the same time, there is a growing need to vision a global carbon monitoring system that can quantify natural and anthropogenic perturbations in the air-sea CO2 flux to quantify and attribute the impacts of ocean CDR efforts on the ocean carbon sink. Here, we propose to present nascent efforts at the Ames Research Center to leverage NASA's unique remote sensing and computational capabilities and expertise in basic and applied Earth science to meet the needs of the ocean CDR stakeholder community as represented by the OceanVisions network. Specifically we aim to develop a global ocean CDR scenario explorer with two objectives: 1) to make quantitative estimates of the air-sea CO2 flux and its uncertainty more accessible to a wide range of users, and 2) to enable these diverse users to interactively explore the uncertain impacts on air-sea CO2 fluxes in a wide range of ocean CDR scenarios. The information would be provided to users via a public graphical user interface, and the results would be based on a new synthesis of scientific observations and knowledge in a global ocean mixed layer inverse model run on supercomputers. The proposed tool aims to fill a unique and valuable niche for users and the scientific community by balancing tradeoffs between user interests, scientific knowledge, and technical capabilities. We will conclude by visioning the potential implications for a global carbon monitoring system in a world with gigaton scale ocean CDR.

Global↗

Contaminant Migration Modeling to Support the In Situ Decommissioning of Hardened Nuclear Facilities - 20215

The decommissioning of hardened nuclear facilities provides a unique challenge in balancing current worker dose with exposure to future receptors and media. In situ decommissioning (ISD) is a cost-effective and safe option for the closure of such facilities but requires a detailed understanding of the threat that radioactive and hazardous constituents remaining in the facilities pose to groundwater and surrounding surface water. The Savannah River Site (SRS) extensively employs contaminant migration models within the decommissioning process to develop site-specific removal, grouting, and monitoring strategies in support of safe and effective final end states of nuclear facilities. This work discusses the use of contaminant migration modeling in the ISD process at SRS, including specific examples from the closure of P-Reactor and R-Reactor, Building 235-F, F-Canyon and F/H Laboratory Complex. Migration models developed during the closure of P-Reactor and R-Reactor were divided into four source areas: the reactor vessel, the process area, the disassembly basin, and the purification wing. Each source area was assigned a specific inventory and migration pathway and was then subject to varying hypothetical removal, capping, and grouting schemes. Modeling indicated that groundwater Maximum Concentration Limits (MCLs) might be exceeded if no action was taken for eleven and ten constituents of concern (COCs) at P-Reactor and R-Reactor, respectively. These exceedances could occur in as few as 200 to 500 years. Alternatively, the migration modeling demonstrated that the selected ISD actions reduced contaminant mobility which allowed for significant radioactive decay and resulted in fewer predicted exceedances of groundwater MCLs (five COCs for P-Reactor; eight COCs for R-Reactor). In response to the modeling results for P-Reactor and R-Reactor, effectiveness monitoring programs were developed to target contaminants, specific to each reactor, that may migrate to groundwater. Contaminant migration modeling also revealed that roof collapse was a large factor in the release of COCs to the environment, giving rise to roof improvements, and an inspection and vegetation control program to ensure roof stability over time. Contaminant migration modeling is also aiding in the closure planning for hardened facilities in F Area. Building 235-F housed the Actinide Billet Line, which produced Np-237 billets for irradiation in SRS reactors, and the Plutonium Fuel Form (PuFF) facility that produced Pu-238 heat sources for the space program. As a result of these missions, areas within Building 235-F contain considerable residual amounts of both Pu-238 and Np-237. Contaminant migration modeling of Building 235-F was originally performed in 2012 to identify the feasibility of ISD and the amount of radioactive material removal required to prevent the exceedance of groundwater MCLs. The original model indicated that a 60% reduction in the PuFF facility Pu-238 inventory could keep groundwater concentrations below standards, while Np-237 did not pose a threat to groundwater. However, updates to the model with an emphasis on source impact pathways revealed that, due to the orientation of the source areas relative to groundwater flow, no amount of reasonable removal of Pu-238 would keep groundwater concentrations below MCLs and that Np-237 could be a large contributor to localized MCL exceedances. With this insight, the refined 2019 model is being used to assist in the development of grouting plans specific to each facility source area, where bentonite may be utilized to slow the migration of Pu-238 and its daughter products from the PuFF facility and a reducing grout may decrease Np-237 transport by ensuring the nuclide remains in the less mobile +IV oxidation state. The beginning phases of contaminant migration modeling are underway for F-Canyon and associated facilities using lessons learned from the Reactors and Building 235-F. A contaminant migration pathway, similar to the pathway used for the reactor vessels, is being developed for the hot and warm canyons. The F-Canyon inventories are being spatially refined to identify specific and localized source zones, comparable to Building 235-F, that may impact groundwater. Contaminant migration modeling provides vital input within the ISD process, from facility investigation to post closure effectiveness monitoring, making it a valuable tool in the development of safe and effective end states for hardened nuclear facilities at SRS. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Evaluation of adjoint-based observation impacts as a function of forecast length using an Observing System Simulation Experiment

Adjoints of numerical weather prediction models may be employed for Forecast Sensitivity to Observation (FSO) in order to monitor the contribution of ingested observation data on short-term forecast skill. However, the calculation of short-term forecast error is difficult due to the lack of a truly independent dataset for verification. In an Observing System Simulation Experiment framework, the Nature Run is able to provide a true and complete verification dataset and allows accurate evaluation of short term forecast errors. In this work, an OSSE developed at the National Aeronautics and Space Administration Global Modeling and Assimilation Office is used to explore the impact of observational data on forecasts in the 6 to 48 hour range. An adjoint of the Global Earth Observing System model is employed to compare the observation impacts estimated using both self-analysis verification and the true Nature Run verification. Self-analysis verification is found to inflate the estimated forecast error growth during the early forecast period, resulting in overestimations of observation impacts, particularly in the 6-12 hour forecast range. By 48 hours, the self-analysis verification estimates of forecast error and observation impacts more closely match the true values. The fraction of beneficial observations is also overinflated at short forecast times when self-analysis verification is used. The progression of impacts of an individual observation or data type depends on the character of the growth of the initial condition error that each observation affects.

numerical weather prediction↗

Development of a Nuclear Fuel Dissolution Monitor Based on Raman Spectroscopy

The processing of spent nuclear fuel and other nuclear materials is a critical component of nuclear material management with implications for global security. The first step of fuel processing is dissolution, with several charges of fuel sequentially added to a batch of solvent. The incomplete dissolution of a charge precludes the addition of the next charge. As the dissolution takes place in a heated, highly corrosive and radiological vessel, direct monitoring of the process is not possible. We discuss the use of Raman spectroscopy to indirectly monitor dissolution through an analysis of the gaseous emissions from the dissolver. Challenges associated with the implementation of Raman spectroscopy include the composition and physical characteristics of the offgas stream and the impact of operating conditions. Nonetheless, we observed that NO2 concentrations serve as a reliable indicator of process activity and correlate to the amount of fuel material that remains undissolved. These results demonstrate the promise of the method for monitoring nuclear material dissolution.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Voluntary Consensus Organization Standards for Nondestructive Evaluation of Thin-Walled Metallic Liners and Composite Overwraps in Composite Overwrapped Pressure Vessels

NASA fracture control requirements outlined in NASA-STD-5009 and NASA-STD-5014 are predicated on the availability and use of sensitive nondestructive evaluation (NDE) methods that can detect and monitor defects, thereby providing data that can be used to predict failure or reduce the risk of failure in fracture critical components. However, in the case of composite materials and components, including composite overwrapped pressure vessels (COPVs), the effect of defects is poorly understood, the NDE methods used to evaluate locate and size defects are typically at lower technical readiness level than analogous NDE methods used for metals, and demonstration studies to verify the probability of detection (POD) are generally lacking or unavailable. These factors together make failure prediction of fracture critical composite materials and components based on size, quantity, or orientation of defects nearly impossible. Also, when inspecting metal liners in as-manufactured COPVs, sensitivity is lost and only the inner surface of the liner is accessible. Also, NDE of COPVs as applied during manufacturing varies significantly from manufacturer to manufacturer and has not yet been standardized. Although requirements exist to perform NDE immediately after manufacturing to establish initial integrity of the parts, procedural detail for NDE of composites is still nonexistent or under development. For example, in practice, only a visual inspection of COPVs is performed during manufacturing and service, leaving in question whether defects of concern, for example, bridging, overwrap winding anomalies, impact damage below visible threshold, out-of-family strain growth, and liner buckling have been adequately detected and monitored. To address these shortcomings, in 2005 the NASA Nondestructive Evaluation Working Group (NNWG) began funding work to develop and adopt standards for nondestructive evaluation of aerospace composites in collaboration with the American Society for Testing and Materials (ASTM) Committee E07 on Nondestructive Testing. Similarly, in 2006 the NASA Engineering and Safety Center (NESC) recommended that nondestructive evaluation methods that can predict composite failure in COPVs should be developed and verified, and integrated into the damage control plan for these vessels

Waller, Jess↗

Assessment of chemo-mechanical impacts of CO 2 sequestration on the caprock formation in Farnsworth oil field, Texas

This study evaluates the chemo-mechanical influence of injected CO 2 on the Morrow B sandstone reservoir and the upper Morrow shale caprock utilizing data from the inverted 5-spot pattern centered on Well 13-10A within the Farnsworth unit (FWU). This study also seeks to evaluate the integrity of the caprock and the long-term CO 2 storage capability of the FWU. The inverted 5-spot pattern was extracted from the field-scale model and tuned with the available field observed data before the modeling work. Two coupled numerical simulation models were utilized to continue the study. First, a coupled hydro-geochemical model was constructed to simulate the dissolution and precipitation of formation minerals by modeling three intra-aqueous and six mineral reactions. In addition, a coupled hydro-geomechanical model was constructed and employed to study the effects of stress changes on the caprock’s porosity, permeability, and ground displacement. The Mohr–Coulomb circle and failure envelope were used to determine caprock failure. In this work, the CO 2 -WAG injection is followed by the historical field-observed strategy. During the forecasting period, a Water Alternating Gas (WAG) injection ratio of 1:3 was utilized with a baseline bottom-hole pressure constraint of 5500 psi for 20 years. A post-injection period of 1000 years was simulated to monitor the CO 2 plume and its effects on the CO 2 storage reservoir and caprock integrity. The simulation results indicated that the impacts of the geochemical reactions on the porosity of the caprock were insignificant as it experienced a decrease of about 0.0003% at the end of the 1000-year post-injection monitoring. On the other hand, the maximum stress-induced porosity change was about a 1.4% increase, resulting in about 4% in permeability change. It was estimated that about 3.3% of the sequestered CO 2 in the formation interacted with the caprock. Despite these petrophysical property alterations and CO 2 interactions in the caprock, the caprock still maintained its elastic properties and was determined to be far from its failure.

04 OIL SHALES AND TAR SANDS↗

Long-Term Geospace Climate Monitoring

Climate change is characterized by global surface warming associated with the increase of greenhouse gas population since the start of the industrial era. Growing evidence shows that the upper atmosphere is experiencing appreciable cooling over the last several decades. The seminal modeling study by Roble and Dickinson (1989) suggested potential effects of increased greenhouse gases on the ionosphere and thermosphere cooling which appear consistent with some observations. However, several outstanding issues remain regarding the role of CO 2 , other important contributors, and impacts of the cooling trend in the ionosphere and thermosphere: for example, (1) what is the regional variability of the trends? (2) the very strong ionospheric cooling observed by multiple incoherent scatter radars that does not fit with the prevailing theory based on the argument of anthropogenic greenhouse gas increases, why? (3) what is the effect of secular changes in Earth’s main magnetic field? Is it visible now in the ionospheric data and can it explain some of the regional variability in the observed ionospheric trends? (4) what is the impact of long-term cooling in the thermosphere on operational systems? (5) what are the appropriate strategic plans to ensure the long-term monitoring of the critical space climate?

long-term trends↗

Predicting ground level impacts of solid rocket motor testing

Beginning in August of 1988 and continuing until the present, NASA at Stennis Space Center, Mississippi has conducted environmental monitoring of selected static test firings of the solid rocket motor used on the Space Shuttle. The purpose of the study was to assess the modeling protocol adapted for use in predicting plume behavior for the Advanced Solid Rocket Motor that is to be tested in Mississippi beginning in the mid-1990's. Both motors use an aluminum/ammonium perchlorate fuel that produces HCl and Al2O3 particulates as the major combustion products of concern. A combination of COMBUS.sr and PRISE.sr subroutines and the INPUFF model are used to predict the centerline stabilization height, the maximum concentration of HCl and Al2O3 at ground level, and distance to maximum concentration. Ground studies were conducted to evaluate the ability of the model to make these predictions. The modeling protocol was found to be conservative in the prediction of plume stabilization height and in the concentrations of the two emission products predicted.

Douglas, Willard L.↗

Additive manufacturing: a challenge to nuclear nonproliferation

Additive manufacturing (AM) is a rapidly developing technology that allows industries to automate and simplify the production of highly complicated items. Recently, there are concerns that AM techniques have the potential to be utilized in the fields of nuclear weapons and nuclear enrichment technology. Presently, there are very few international or domestic export controls that apply to AM's role in the nuclear industry, constituting an unmanaged proliferation pathway. Pre-existing export controls focus on general concepts and processes and do not consider specific nuances of various techniques that are categorized as additive manufacturing. To introduce legislation and controls that will be effective in monitoring proliferation pathways, one must investigate and characterize AM techniques and their nuclear applications. This paper categorizes and ranks 33 AM techniques based on their potential impact on the nuclear fuel cycle and the development of nuclear weapons. Through this method of characterization and categorization, export controls would address specific AM nuclear proliferation risks without disrupting the entire industry and fuel cycle. Additionally, legislation employing this method would identify loopholes in export controls using a holistic approach to managing and monitoring proliferation pathways.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗

Photonic methods and technologies for monitoring the ocean and atmosphere

In this paper, we review photonic methods and technologies that are promising for monitoring the ocean and atmosphere and have been implemented mainly in recent years at the Institute of Automation and Control Processes, Far Eastern Branch, Russian Academy of Sciences. We present results of lidar studies that have made it possible to understand key features of ocean – atmosphere interaction processes under continent – ocean transition conditions, which determine specific features of the atmospheric aerosol distribution, small gaseous components of the atmosphere and its optical characteristics. We consider methods and tools for combined optical and laser fluorescence monitoring of the ocean surface. Particular attention is paid to results of research on remote methods and tools for real-time laser-induced and laser fluorescence environmental monitoring of the ocean, including specialised fibre-optic probes and mobile underwater robotic systems. We present results of the development and investigation of highly sensitive, noise-proof fibre-optic hydro- and seismic/acoustic sensors for remote monitoring of the ocean and robotic systems for underwater laser protection of marine vessels, hydraulic structures and oceanographic instruments against the negative impact of biofouling. (invited paper)

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Optical Sensing using Fiber Bragg Gratings for Monitoring Structural Damage in Composite Over-Wrapped Vessels

Composite Over-Wrap Vessels are widely used in the aerospace community. They are made of thin-walled bottles that are over wrapped with high strength fibers embedded in a matrix material. There is a strong drive to reduce the weight of space borne vehicles and thus pushes designers to adopt COPVs that are over wrapped with graphite fibers embedded in its epoxy matrix. Unfortunately, this same fiber-matrix configuration is more susceptible to impact damage than others and to make matters worse; there is a regime where impacts that damage the over wrap leave no visible scar on the COPV surface. In this paper FBG sensors are presented as a means of monitoring and detecting these types of damage. The FBG sensors are surface mounted to the COPVs and optically interrogated to explore the structural properties of these composite pressure vessels. These gratings optically inscribed into the core of a single mode fiber are used as a tool to monitor the stress strain relation in the composite matrix. The response of these fiber-optic sensors is investigated by pressurizing the cylinder up to its burst pressure of around 4500 psi. A Fiber Optic Demodulation System built by Blue Road Research, is used for interrogation of the Bragg gratings.

Grant, Joseph↗

"Designing, simulating, and performing the 100-AV field test for the CIRCLES consortium: Methodology and Implementation of the Largest mobile traffic control experiment to date"

Previous controlled experiments on single-lane ring roads have shown that a single partially autonomous vehicle (AV) can effectively mitigate traffic waves. This naturally prompts the question of how these findings can be generalized to field operational, high-density traffic conditions. To address this question, the Congestion Impacts Reduction via CAV-in-the-loop Lagrangian Energy Smoothing (CIRCLES) Consortium conducted MegaVanderTest (MVT), a live traffic control experiment involving 100 vehicles near Nashville, TN, USA. This article is a tutorial for developing analytical and simulation-based tools essential for designing and executing a live traffic control experiment like the MVT. It presents an overview of the proposed roadmap and various procedures used in designing, monitoring, and conducting the MVT, which is the largest mobile traffic control experiment at the time. The design process is aimed at evaluating the impact of the CIRCLES AVs on surrounding traffic. The article discusses the agent-based traffic simulation framework created for this evaluation. A novel methodological framework is introduced to calibrate this microsimulation, aiming to accurately capture traffic dynamics and assess the impact of adding 100 vehicles to existing traffic. The calibration model's effectiveness is verified using data from a six-mile section of Nashville's I-24 highway. The results indicate that the proposed model establishes an effective feedback loop between the optimizer and the simulator, thereby calibrating flow and speed with different spatiotemporal characteristics to minimize the error between simulated and real-world data. Finally, We simulate AVs in multiple scenarios to assess their effect on traffic congestion. This evaluation validates the AV routes, thereby contributing to the execution of a safe and successful live traffic control experiment via AVs.

Ameli, Mostafa↗

Real-time cure monitoring of fiber-reinforced polymer composites using infrared thermography and recursive Bayesian filtering

Despite the desirable characteristics of fiber-reinforced polymer (FRP) composites, their utilization in high volume production industries is limited by a lack of efficient manufacturing techniques. Monitoring the curing process of these composites can help with improving the quality and efficiency of the manufacturing process. This article discusses a method that uses Kalman-filter-based fusion of the information obtained from infrared thermography (surface temperature measurements) and a heat conduction model to estimate degrees of cure and internal temperatures of curing FRP composite parts in real time. The effectiveness of the methodology is demonstrated by successfully monitoring the curing of an FRP composite part in a laboratory experiment. Finally, the proposed methodology is a crucial step towards identifying anomalies in the curing process that negatively impact the quality of FRP composite parts.

36 MATERIALS SCIENCE↗

Geophysical Characterization and Monitoring of the 200 Area Treated Effluent Disposal Facility to Support Permit Renewal; TEDF Geophysical Monitoring

The Treated Effluent Disposal Facility (TEDF), located in the 200 East Area of the Hanford Site, is a site where non-hazardous and non-radioactive liquid wastes are disposed into two state-permitted infiltration basins. In 2016, the Washington State Department of Ecology denied a permit renewal request for TEDF due to the inability to adequately assess the impact of TEDF discharge water on the underlying groundwater quality. TEDF overlies the relatively impermeable Ringold Lower Mud (RLM) unit, whose upper contact lies in the vadose zone approximately 30 m below ground surface and approximately 10 m above the water table boundary. The RLM is assumed to isolate TEDF discharge water from the natural groundwater aquifer, which is monitored using wellbores screened below the RLM. Therefore, samples collected from monitoring wells near TEDF are not considered representative of TEDF discharge water. Rather, TEDF discharge water is assumed to mound on top of the RLM to form a perched aquifer. To support permitting of the TEDF, a new monitoring well is required that can be used to sample water from the presumed perched water aquifer above the RLM. Ideally, the screened section of the well would be located at the peak of the perched water mound(s), which presumably occurs at the point of maximum vertical flux from TEDF to the RLM, or equivalently where the dominant infiltration flow paths reach the RLM. This report describes how time-lapse 3D electrical resistivity tomography (ERT) was used in conjunction with nominal TEDF discharge operations to image the dominant flow paths from each pond to the RLM. Results are summarized in Figure ES.1. Figure ES.1A shows a satellite image of the TEDF overlain by an array of surface ERT electrodes. The solid and dashed black circles denote the zones of maximum vertical flux at the RLM within the south and north ponds, respectively, and presumably the regions where perched water peaks during discharge. Figure ES.1B shows time-lapse difference imaging results approximately 15 days after switching discharge from the south pond to the north pond. Blue iso-surfaces beneath the north pond denote regions of increasing bulk electrical conductivity caused by increasing saturation due to infiltrating water. Red iso-surfaces beneath the south pond denote regions that were previously saturated during the south pond discharge and are now de-saturating, causing a decrease in bulk electrical conductivity. In both cases, the zones of maximum change mark the dominant flow paths to the RLM. Figure ES.1C shows time-lapse imaging results approximately 15 days after switching discharge from the north pond to the south pond. In this case, blue iso-surfaces mark the dominant flow paths to the RLM from the south pond. The red iso-surfaces mark the dominant flow paths that existed during discharge to the north pond. The regions of maximum change in bulk conductivity (due to saturation or desaturation) that mark the primary flow paths are equivalent in both cases and denoted by the dashed solid and black circles. If a perched water zone forms on the RLM, it is likely to mound within or near the dashed circle during discharge to the north pond, and in the solid circle during discharge to the south pond. In other words, if perched water mounding occurs, the circles mark the optimum locations suggested by the ERT imaging for monitoring boreholes to be placed, enabling samples to be collected that are representative of TEDF discharge impacts on groundwater quality.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Prevalence of human papillomavirus genotypes in high-grade cervical precancer and invasive cervical cancer from cancer registries before and after vaccine introduction in the United States

Background. US population-based cancer registries can be used for surveillance of human papillomavirus (HPV) types found in HPV-associated cancers. Using this framework, HPV prevalence among high-grade cervical precancers and invasive cervical cancers were compared before and after HPV vaccine availability. Methods. Archived tissue from 2 studies of cervical precancers and invasive cervical cancers diagnosed from 1993-2005 (prevaccine) were identified from 7 central cancer registries in Florida; Hawaii; Iowa; Kentucky; Louisiana; Los Angeles County, California; and Michigan; from 2014 through 2015 (postvaccine) cases were identified from 3 registries in Iowa, Kentucky, and Louisiana. HPV testing was performed using L1 consensus polymerase chain reaction analysis. HPV-type–specific prevalence was examined grouped by hierarchical attribution to vaccine types: HPV 16, 18, HPV 31, 33, 45, 52, 58, other oncogenic HPV types, and other types/HPV negative. Generalized logit models were used to compare HPV prevalence in the prevaccine study to the postvaccine study by patient age, adjusting for sampling factors. Results. A total of 676 precancers (328 prevaccine and 348 postvaccine) and 1140 invasive cervical cancers (777 prevaccine and 363 postvaccine) were typed. No differences were observed in HPV-type prevalence by patient age between the 2 studies among precancers or invasive cancers. Conclusions. The lack of reduction in vaccine-type prevalence between the 2 studies is likely explained by the low number of cases and low HPV vaccination coverage among women in the postvaccine study. Monitoring HPV-type prevalence through population-based strategies will continue to be important in evaluating the impact of the HPV vaccine.

59 BASIC BIOLOGICAL SCIENCES↗

Multivariate environmental and trait-based controls of transpiration in the Central Amazon Rainforest

Tropical forest tree mortality is increasing due to more severe droughts, yet our understanding of how tree traits and life strategies are linked to drought stress has been limited by measurement scarcity. The BIONTE (BIOmass and NuTrient Experiment) near Manaus, Brazil hosts one of the world’s largest sap flow installations, with sensors in 90 canopy trees across a wood density gradient monitored since June 2022. The 2023 El Niño drought provided a unique opportunity to evaluate how water availability impacts tree transpiration. An interpretable machine learning framework was used to study the complex interactions between transpiration and multiple environmental variables such as soil water availability and vapor pressure deficit (VPD), and how these interactions vary with wood density and individual trees. We found varying responses of transpiration from different trees during the El Niño drought. Transpiration generally increased with temperature, with stronger effects in wetter areas and in trees with low to medium wood density. However, this response was modulated by stomatal sensitivity to VPD, which constrained transpiration under high atmospheric demand, particularly in intermediate-moisture area. The inflection in transpiration rate at high temperatures (>32°C) underscores the role of stomatal and hydraulic regulation in limiting water loss and protecting trees from excessive evaporative demand. Analysis of soil water contribution to transpiration revealed unimodal patterns in wetter area, with peak contributions near 0.45 cm 3 cm -3 of surface soil water and declining or flat responses beyond that threshold, suggesting a shift from water- to energy-limited transpiration. In contrast, drier areas exhibited limited transpiration sensitivity to soil water conditions and minimal trait-based variation in VPD responses, indicating supply-limited conditions. Despite higher wood density trees being generally more resilient, this study shows diverse tree drought resilience, prompting further investigation into the specific traits and dynamics between environmental variables in regulating transpiration and other physiological processes in trees.

Drought↗

Significance and complications of drilling fluid rheology in geothermal drilling: A review

The harsh downhole conditions of high pressure and high temperature (HPHT) encountered in geothermal wells make the drilling operation challenging. Drilling in such environments requires a special drilling mud formulation with high thermal stability and good rheological properties to fulfill the drilling fluid functions. Therefore, great efforts should be put into selecting the suitable drilling fluid, optimize and monitor the drilling fluid properties throughout drilling operations, and predicting its performance under downhole conditions. Rheological properties significantly impact many drilling parameters such as hole cleaning, fluid and wellbore stability, wellbore hydraulics, torque and drag, and other drilling issues. This paper discusses water-based drilling fluids' flow behavior under HPHT conditions and highlights the significance of fluid rheology in geothermal drilling. The common challenges and complications related to fluid rheology encountered in geothermal drilling are addressed in this paper, such as hole cleaning, wellbore hydraulics, and drilling fluid stability. This article also reviews the recent advances in drilling mud systems, rheology enhancement, and rheological properties measurements at surface and subsurface conditions. Furthermore, the rheology models of drilling fluid at elevated temperatures are reviewed to fully understand their flow behavior and establish a method for drilling engineers to optimize fluid formulations for geothermal drilling.

58 GEOSCIENCES↗