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At least 847 records · Page 47
All sourcesNASA NTRSDOE OSTI
DOE OSTI2019

Cross section for γ n → π 0 n measured at the Mainz A2 experiment

The γn → π 0 n differential cross section evaluated for 27 energy bins span the photon-energy range 290–813 MeV (W = 1.195 – 1.553 GeV) and the pion c.m. polar production angles, ranging from 18° to 162°, making use of model-dependent nuclear corrections to extract π 0 production data on the neutron from measurements on the deuteron target. Additionally, the total photoabsorption cross section was measured. The tagged photon beam produced by the 883-MeV electron beam of the Mainz Microtron MAMI was used for the π 0 -meson production. Our accumulation of 3.6×10 6 γn → π 0 n events enabled a detailed study of the reaction dynamics. Our data are in reasonable agreement with previous A2 measurements and extend them to lower energies. The data are compared to predictions of previous SAID, MAID, and BnGa partial-wave analyses and to the latest SAID fit MA19 that included our data. Selected photon decay amplitudes N* → γn at the resonance poles are observed for the first time.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2022

Voltage and Current Response Characteristics of PMU Device by PCI Simulation

Phasor Measurement Units (PMUs) are critical for the Wide Area Measurement System (WAMS), especially for real-time grid monitoring, disturbance location, and situation awareness. Once they are damaged, the stable operation of power system will be affected directly and greatly. As new electronic devices in power system, their immunity to electromagnetic pulse (EMP) events needs to be studied. In this paper, an effective impedance measurement scheme is proposed for port impedance measurement of PMU. A non-uniform transmission line model is established to eliminate the impact of fixture in the de-embedding process. Then circuit of pulse current generator is established to generate damping sinusoid and double exponential wave applied to the port. Finally, by using measured impedance as load of the generator, the voltage and current responses of different ports are calculated in the pulsed current injection (PCI) simulation. Results shows the characteristics of port impedance, voltage and current waveforms. The relation between port impedance and the waveforms is discussed.

Zhang, Liang↗
DOE OSTI2024

Catabolic pathway acquisition by rhizosphere bacteria readily enables growth with a root exudate component but does not affect root colonization

Horizontal gene transfer (HGT) is a fundamental evolutionary process that plays a key role in bacterial evolution. The likelihood of a successful transfer event is expected to depend on the precise balance of costs and benefits resulting from pathway acquisition. Most experimental analyses of HGT have focused on phenotypes that have large fitness benefits under appropriate selective conditions, such as antibiotic resistance. However, many examples of HGT involve phenotypes that are predicted to provide smaller benefits, such as the ability to catabolize additional carbon sources. We have experimentally simulated the consequences of one such HGT event in the laboratory, studying the effects of transferring a pathway for catabolism of the plant-derived aromatic compound salicyl alcohol between rhizosphere isolates from the Pseudomonas genus. We find that pathway acquisition enables rapid catabolism of salicyl alcohol with only minor disruptions to the existing metabolic and regulatory networks of the new host. However, this new catabolic potential does not confer a measurable fitness advantage during competitive growth in the rhizosphere. We conclude that the phenotype of salicyl alcohol catabolism is readily transferable but is selectively neutral under environmentally relevant conditions. We propose that this condition is common and that HGT of many pathways will be self-limiting because the selective benefits are small.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Revealing systematic changes in the transcriptome during the transition from exponential growth to stationary phase

ABSTRACT The composition of bacterial transcriptomes is determined by the transcriptional regulatory network (TRN). The TRN regulates the transition from one physiological state to another. Here, we use independent component analysis to monitor the composition of the transcriptome during the transition from the exponential growth phase to the stationary phase. With Escherichia coli K-12 MG1655 as a model strain, we trigger the transition using carbon, nitrogen, and sulfur starvation. We find that (i) the transition to the stationary phase accompanies common transcriptome changes, including increased stringent responses and reduced production of cellular building blocks and energy regardless of the limiting element; (ii) condition-specific changes are strongly associated with transcriptional regulators ( e.g. , Crp, NtrC, CysB, Cbl) responsible for metabolizing the limiting element; and (iii) the shortage of each limiting element differentially affects the production of amino acids and extracellular polymers. This study demonstrates how the combination of genome-scale datasets and new data analytics reveals the fundamental characteristics of a key transition in the life cycle of bacteria. IMPORTANCE Nutrient limitations are critical environmental perturbations in bacterial physiology. Despite its importance, a detailed understanding of how bacterial transcriptomes are adjusted has been limited. By utilizing independent component analysis (ICA) to decompose transcriptome data, this study reveals key regulatory events that enable bacteria to adapt to nutrient limitations. The findings not only highlight common responses, such as the stringent response, but also condition-specific regulatory shifts associated with carbon, nitrogen, and sulfur starvation. The insights gained from this work advance our knowledge of bacterial physiology, gene regulation, and metabolic adaptation.

Lim, Hyun Gyu (ORCID:0000000204692388)↗
DOE OSTI2021

Modeling Growth Kinetics, Interspecies Cell Fusion, and Metabolism of a Clostridium acetobutylicum / Clostridium ljungdahlii Syntrophic Coculture

ABSTRACT Clostridium acetobutylicum and Clostridium ljungdahlii grown in a syntrophic culture were recently shown to fuse membranes and exchange cytosolic contents, yielding hybrid cells with significant shifts in gene expression and growth phenotypes. Here, we introduce a dynamic genome-scale metabolic modeling framework to explore how cell fusion alters the growth phenotype and panel of metabolites produced by this binary community. Computational results indicate C. ljungdahlii persists in the coculture through proteome exchange during fusing events, which endow C. ljungdahlii cells with expanded substrate utilization, and access to additional reducing equivalents from C. acetobutylicum -evolved H 2 and through acquisition of C. acetobutylicum -native cofactor-reducing enzymes. Simulations predict maximum theoretical ethanol and isopropanol yields that are increased by 0.64 mmol and 0.39 mmol per mmol hexose sugar consumed, respectively, during exponential growth when cell fusion is active. This modeling effort provides a mechanistic explanation for the metabolic outcome of cellular fusion and altered homeostasis achieved in this syntrophic clostridial community. IMPORTANCE Widespread cell fusion and protein exchange between microbial organisms as observed in synthetic C. acetobutylicum / C. ljungdahlii culture is a novel observation that has not been explored in silico . The mechanisms responsible for the observed cell fusion events in this culture are still unknown. In this work, we develop a modeling framework that captures the observed culture composition and metabolic phenotype, use it to offer a mechanistic explanation for how the culture achieves homeostasis, and identify C. ljungdahlii as primary beneficiary of fusion events. The implications for the events described in this study are far reaching, with potential to reshape our understanding of microbial community behavior synthetically and in nature.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Lake Huron Enhances Snowfall Downwind of Lake Erie: A Modeling Study of the 2010 New Year’s Lake Effect Snowfall Event

In the Northeast and Great Lakes regions of the United States, the influence of multiple lakes on the overlying air can greatly affect lake-effect snowfall over downwind communities. To assess the impact of Lake Huron on snowfall downwind of Lake Erie, we simulated a lake-effect snow event which occurred from 1 to 6 January 2010 using the Regional Atmospheric Modeling System (RAMS). We found that the presence of Lake Huron enhances snowfall downwind of Lake Erie by almost 20% and leads to much heavier local snowfall totals than when Lake Huron is not present. This increase in snowfall is due to a lake-to-lake (L2L) convective band, as secondary circulations associated with lake-effect convection form over Lake Huron and persist overland between the lakes before reintensifying over Lake Erie. As these secondary circulations move over Lake Erie, lifting within the upward branch of the circulations accelerates the development of convection within the L2L band. Surface fluxes and convection over Lake Huron deepen the boundary layer, promoting deeper vertical development of this L2L band over Lake Erie. However, although this boundary layer modification strengthens the L2L band, we found that it actually reduced lake-effect snowfall produced by wind-parallel bands (WPB) in other parts of the lake. This indicates that boundary layer modification from upstream lakes may impact L2L bands differently than WPB which are not connected to upstream secondary circulations.

Lake effects↗
DOE OSTI2022

Financial Analysis of Experimental Releases Conducted at Glen Canyon Dam during Water Year 2021

This report was prepared by Argonne National Laboratory (Argonne) in support of a financial analysis of two Glen Canyon Dam (GCD) flow experiments that were conducted in Water Year 2021. The first experiment, called “spring disturbance flow”, was conducted from March 15 to 26, 2021. This disturbance flow experiment is designed to simulate a spring-timed runoff event so scientists can study its effects on the Colorado River ecosystem. The second experiment was a reduced and steady discharge from May 29 to June 4, 2021. This reduced steady flow experiment was conducted for the United States Geological Survey (USGS) to collect high-resolution aerial imagery over Grand Canyon National Park. This analysis was funded by the Colorado River Storage Project (CRSP) Office of the U.S. Department of Energy’s Western Area Power Administration (WAPA). CRSP markets electricity produced by hydroelectric facilities collectively known as the Salt Lake City Area Integrated Projects including dams equipped for power generation on the Colorado, Green, Gunnison, and Rio Grande Rivers and on Plateau Creek in the states of Arizona, Colorado, New Mexico, Utah, and Wyoming. Staff members in Argonne’s Energy Systems Division prepared this technical memorandum with assistance from WAPA’s CRSP and Energy Marketing and Management Offices (EMMO).

58 GEOSCIENCES↗
DOE OSTI2026

Genomic-based biosurveillance for avian influenza: whole genome sequencing from wild mallards sampled during autumn migration in 2022–2023 reveals a high co-infection rate on migration stopover site in Georgia

The Caucasus region, including Georgia, is an important intersection for migratory waterbirds, offering potential for avian influenza virus (AIV) transmission between populations from different geographic areas. In 2022 and 2023, wild ducks were sampled during autumn migration events in Georgia to study the genetic relationships and molecular characteristics of influenza strains. Sequencing and phylogenetic analysis were used to compare the sampled strains to reference sequences from Africa, Asia, and Europe, allowing assessment of genetic relationships and virus transmission between migratory birds. Protein language modeling identified potential co-infections. Of 225 duck samples, 128 tested positive for the influenza M gene. 55 influenza-positive samples underwent whole-genome sequencing, revealing significant diversity. Analysis of the hemagglutinin (HA) segment showed notable differences among subtypes. Most samples were H6N1 and H6N6, but co-infections with combinations like H6H3, N8N1, N6H9, N2N6, and H9H6/N1N2 were also identified. These findings demonstrate the high variability of influenza viruses in migratory waterbirds in Georgia, including a notable rate of co-infections. Some samples exhibited uncommon genetic characteristics compared to other strains from the same year, suggesting Georgia’s role as a mixing vessel for influenza viruses. This facilitates reassortment during co-infections and contributes to the genetic diversity observed across flyways.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

A Machine Learning–Based Approach to Time-series Wave Identification in the Solar Wind

The Wind spacecraft has yielded several decades of high-resolution magnetic field data, a large fraction of which displays small-scale structures. In particular, the solar wind is full of wavelike fluctuations that appear in both the field magnitude and its components. The nature of these fluctuations can be tied to the properties of other structures in the solar wind, such as shocks, that have implications for the time evolution of the solar wind. As such, having a large collection of wave events would facilitate further study of the effects that these fluctuations have on solar wind evolution. Given the large volume of magnetic field data available, machine learning is the most practical approach to classifying the myriad small-scale structures observed. To this end, a subset of Wind data is labeled and used as a training set for a multibranch 1D convolutional neural network aimed at classifying circularly polarized wave modes. Using this algorithm, a preliminary statistical study of 1 yr of data is performed, yielding about 300,000 wave intervals out of about 5,000,000 solar wind intervals. The wave intervals come about more often in the fast solar wind and at higher temperatures, and the number of waves per day is highly periodic. This machine learning–based approach to wave detection has the potential to be a powerful, inexpensive way to catalog waves throughout decades of spacecraft data.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS1973

A high resolution study in time, position, intensity, and frequency of a radio event on 14 January 1971

High resolution position and spectrum measurements were made of a complex solar radio activity on January 14, 1971, from 1120 to 1130 UT. Positions were measured with 169 MHz Nancay E-W Radioheliograph, while dynamic spectra and polarization were observed with the 60-channel radiospectrograph of Nera. The radio event consisted of two closely similar groups of type 3 bursts and type 2-like details, and a stationary type 4 continuum, including some very clear pulsating structures. The general aspect of this activity is presented.

Caroubalos, C.↗
NASA NTRS1973

A study of the break-up characteristics of Chena River Basin using ERTS imagery

The author has identified the following significant results. The Chena River Basin was selected because of the availability of ground truth data for comparison. Very good agreement for snow distribution and rates of ablation was found between the ERTS-1 imagery, the snowmelt model, and field measurements. Monitoring snowmelt rates for relatively small basins appears to be practical. The main limitation of the ERTS-1 imagery is the interval of coverage. More frequent overflights providing coverage are needed for the study of transient hydrologic events. ERTS-1 data is most useful when used in conjunction with snowmelt prediction models and existing snow course data. These results should prove very useful in preliminary assessment of hydrologic conditions in ungaged watersheds and will provide a tool for month-to-month volume forecasting.

Carlson, R. F.↗
NASA NTRS1974

Feedback precision and postfeedback interval duration

Precision of feedback gain was manipulated in a simple positioning task. An optimum was found; an increase in precision past that optimum produced deleterious effects upon rate of acquisition. In a second study, increasing postfeedback interval removed that optimum. The feedback precision effects were then replicated in a timing task. The combined results of the 3 studies were interpreted as supportive of an information-processing approach to the study of postfeedback interval events for simple motor skills. The findings additionally supported specific predictions by Bilodeau and deductions from Adams' 1971 theory of motor learning.

Rogers, C. A., Jr.↗
NASA NTRS1975

Micropulsations in the morning sector. II - Satellite observations of 10to 45-s waves at synchronous orbit, ATS 1

A study of 13 wave events having 10- to 45-s periods and observed by ATS 1 in the morning sector reveals that these events, which have been called band-limited pulsations and are thought to be related to Pi 1, appear actually to be Pc 3. Digital power-spectral and coherency analysis was used to determine the characteristics of the wave activity. The average peak frequency of the events (about 0.04 Hz) and their generally narrow-band nature correspond closely with the characteristics of Pc 3. The local-time distribution of the events (peaking at 0900 LT) and their less than complete association with substorms also correspond more closely with Pc 3 characteristics. The orientation of the major axis of the polarization ellipse for these events was predominantly azimuthal, which agrees more closely with the east-west polarization of Pi 1 observed on the ground. However, some examples of radial orientation were observed in the satellite data.

Arthur, C. W.↗
NASA NTRS1979

Topoclimatological and snowhydrological survey of Switzerland

The author has identified the following significant results. The chosen time of overflight of HCMM seems to be ideal for the study of basic climatological events. Nocturnal temperature inversion zones are easily detectable and their dependency on the relief is clearly seen. Especially the alpine valleys show a very differentiated pattern of cold lakes, separated by warmer zones as a consequence of rock and forrest barriers or changes in the valley profile. Wet areas are usually colder than dry parts under comparable topographic conditions. Even very small topographic obstacles are able to block up the flow of cold air masses (or ground water flow). Urban areas are clearly detectable. Differences to the surface temperatures of surrounding rural land are much more significant during day-time (mainly during the summer months). Fog layers are clearly defined in the visible channel (day-time), but much more difficult to identify in the IR (mainly during the night). There is not a fundamental difference to NOAA-analysis of cloud systems. The most important advantage is the better detectability of convective cloud systems (small cumulus clouds).

Winiger, M.↗
NASA NTRS1979

Plasma wave turbulence associated with an interplanetary shock

The present paper deals with interplanetary shocks, detected and analyzed to date, from the Helios 1 and 2 spacecraft in eccentric solar orbits. The plasma wave turbulence associated with the shock observed on March 30, 1976 is studied in detail. This event is of particular interest because it represents a clearly defined burst of turbulence against a quiet solar wind background both upstream and downstream of the shock. The shock itself is an oblique shock with upstream parameters characterized by a low Mach number, a low beta, and an abnormally large electron to ion temperature ratio. The types of plasma wave detected are discussed.

Gurnett, D. A.↗
NASA NTRS1982

Environmentally induced discharges on satellites

The problem of assessing hazards to geosynchronous satellite systems from geomagnetic substorm encounters is investigated. The available space flight data, coupled with analytical modeling studies, show that only relatively low differential charging is possible from environmental encounters. Using an analytical study of a discharge event on SCATHA, a discharge process is postulated where a small amount of charge is lost to space. These characteristics could then be used as inputs to a coupling model to determine the hazard to a spacecraft. The procedure is applied to a three axis stabilized satellite design.

Stevens, N. J.↗
NASA NTRS1984

Numerical Prediction of the President's Day Cyclone

Numerical-diagnostic studies of major anomalous weather events are being conducted in order to investigate the relevant physical processes associated with the events and the role of satellite observing systems in the analysis and prediction of these phenomena. One component of this study is concerned with the numerical prediction of intense coastal and oceanic cyclogenesis. The specific objectives of this research are: (1) to assess the accuracy of Goddard Laboratory for Atmospheric Sciences model predictions of cyclogenesis, (2) to determine the importance of large scale dynamical processes and diabatic heating to the prediction, and (3) to evaluate the sensitivity of the model predictions to the initial conditions. It was shown that diabatic heating resulting from oceanic fluxes significantly contributed to the generation of low level cyclonic vorticity and the intensification and slow rate movement of an upper level ridge over the western Atlantic. As an upper level short-wave trough approached this ridge, diabatic heating associated with the release of latent heat intensified, and the gradient of vorticity, vorticity advection, upper level divergence, and upward vertical motion in advance of the trough were greatly increased, providing strong large-scale forcing for the surface cyclogenesis.

Atlas, R.↗
NASA NTRS1986

Accelerated plasma flows at the near-tail magnetopause

ISEE-1 and -2 fast plasma data of ion and electron distributions at 16 energies and 16 velocities are used to study accelerated particle flow events at the near-tail dusk magnetopause. The flows separated the plasma sheet from the magnetosheath, a situation which normally arises when the local magnetosheath and plasma sheet magnetic fields are close to being antiparallel. The flows were directed tailward at speeds up to twice those in the adjoining magnetosheath and had densities similar to those in the magnetosheath. The attendant ion and electron temperatures were between those in the magnetosheath and in the plasma sheet. The flow region closest to the magnetosheath had the highest velocity and the lowest density, and a lowered-density field line region appeared on the earthward side of the accelerated flow region. The flows had the velocity changes of a tangential stress balance and are taken as the location of field line merging near (and perhaps tailward) of the dawn-dusk terminator. Significantly fewer accelerated flows are seen in the dawn tail magnetopause, an asymmetry attributed to a seasonally dependent warping of the midtail neutral sheet or a superimposition of interplanetary magnetic field lines over the dusk magnetopause.

Gosling, J. T.↗