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At least 847 records · Page 47

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DOE OSTI2016

Quorum Quenching of Nitrobacter winogradskyi Suggests that Quorum Sensing Regulates Fluxes of Nitrogen Oxide(s) during Nitrification

ABSTRACT Quorum sensing (QS) is a widespread process in bacteria used to coordinate gene expression with cell density, diffusion dynamics, and spatial distribution through the production of diffusible chemical signals. To date, most studies on QS have focused on model bacteria that are amenable to genetic manipulation and capable of high growth rates, but many environmentally important bacteria have been overlooked. For example, representatives of proteobacteria that participate in nitrification, the aerobic oxidation of ammonia to nitrate via nitrite, produce QS signals called acyl-homoserine lactones (AHLs). Nitrification emits nitrogen oxide gases (NO, NO 2 , and N 2 O), which are potentially hazardous compounds that contribute to global warming. Despite considerable interest in nitrification, the purpose of QS in the physiology/ecology of nitrifying bacteria is poorly understood. Through a quorum quenching approach, we investigated the role of QS in a well-studied AHL-producing nitrite oxidizer, Nitrobacter winogradskyi . We added a recombinant AiiA lactonase to N. winogradskyi cultures to degrade AHLs to prevent their accumulation and to induce a QS-negative phenotype and then used mRNA sequencing (mRNA-Seq) to identify putative QS-controlled genes. Our transcriptome analysis showed that expression of nirK and nirK cluster genes ( ncgABC ) increased up to 19.9-fold under QS-proficient conditions (minus active lactonase). These data led to us to query if QS influenced nitrogen oxide gas fluxes in N. winogradskyi . Production and consumption of NO x increased and production of N 2 O decreased under QS-proficient conditions. Quorum quenching transcriptome approaches have broad potential to identify QS-controlled genes and phenotypes in organisms that are not genetically tractable. IMPORTANCE Bacterial cell-cell signaling, or quorum sensing (QS), is a method of bacterial communication and gene regulation that is well studied in bacteria. However, little is known about the purpose of QS in many environmentally important bacteria. Here, we demonstrate quorum quenching coupled with mRNA-Seq to identify QS-controlled genes and phenotypes in Nitrobacter winogradskyi , a nitrite-oxidizing bacterium. Nitrite oxidizers play an important role in the nitrogen cycle though their participation in nitrification, the aerobic oxidation of ammonia to nitrate via nitrite. Our quorum quenching approach revealed that QS influences production and consumption of environmentally important nitrogen oxide gases (NO, NO 2 , and N 2 O) in N. winogradskyi . This study demonstrated a novel technique for studying QS in difficult-to-work-with microorganisms and showed that nitrite oxidizers might also contribute to nitrification-dependent production of nitrogen oxide gases that contribute to global warming.

Mellbye, Brett L.↗

DOE OSTI2015

Energy of interaction between carbon impurities in paramagnetic γ-iron

The energies of interaction between carbon impurity atoms in paramagnetic fcc iron (austenite) are calculated using electron density functional theory. Point defects in the paramagnetic matrix are described using a statistical approach that takes into account local magnetic fluctuations and atomic relaxation in the environment of impurity atoms. It is shown that, in addition to local deformations, magnetism significantly contributes to the energies of dissolution and interaction of carbon atoms. The values of the carbon-carbon interaction energy are indicative of a significant repulsion between these atoms in the first and second coordination spheres. The results of calculations are consistent with estimates obtained from experimental data on the activity of carbon impurity atoms in iron.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

DOE OSTI2024

Maritime Battery Electrification Simulator (MariBES) v1

MariBES is a Python-based software designed for calculating emissions and energy consumption in maritime transportation. This software is capable of performing calculations for multiple vessels, facilitating emission analysis at regional, national, and international scales. It also allows for the examination of energy consumption under various resource such as heavy fuel oil, diesel, and battery-electric, enabling the assessment of different decarbonization strategies in the maritime sector. MariBES utilizes public data on ship activities combined with detailed vessel specifications, significantly enhancing the accuracy of its simulations. This approach marks a considerable advancement over previous models that were constrained by limited spatial and temporal resolution. It features a temporal resolution based on 5-minute intervals and a spatial resolution using precise coordinates.

Moon, HeeSeung↗

DOE OSTI2023

Solving multiphysics-based inverse problems with learned surrogates and constraints

Abstract Solving multiphysics-based inverse problems for geological carbon storage monitoring can be challenging when multimodal time-lapse data are expensive to collect and costly to simulate numerically. We overcome these challenges by combining computationally cheap learned surrogates with learned constraints. Not only does this combination lead to vastly improved inversions for the important fluid-flow property, permeability, it also provides a natural platform for inverting multimodal data including well measurements and active-source time-lapse seismic data. By adding a learned constraint, we arrive at a computationally feasible inversion approach that remains accurate. This is accomplished by including a trained deep neural network, known as a normalizing flow, which forces the model iterates to remain in-distribution, thereby safeguarding the accuracy of trained Fourier neural operators that act as surrogates for the computationally expensive multiphase flow simulations involving partial differential equation solves. By means of carefully selected experiments, centered around the problem of geological carbon storage, we demonstrate the efficacy of the proposed constrained optimization method on two different data modalities, namely time-lapse well and time-lapse seismic data. While permeability inversions from both these two modalities have their pluses and minuses, their joint inversion benefits from either, yielding valuable superior permeability inversions and CO 2 plume predictions near, and far away, from the monitoring wells.

Yin, Ziyi (ORCID:0000000250248771)↗

DOE OSTI2017

Measurements on the Diffusion Length of Thermal Neutrons in Water from 25 to 296°C

Here, the diffusion length of thermal neutrons has been measured in water over the range 25.9 to 295.3°C in the pressure vessel of the KAPL High Temperature Critical Assembly. The diffusion length was determined by fitting an exponential to the data found by activating manganese foils with neutrons from a small Sb-Be source. The temperature variation of the diffusion length could be fairly well represented by either of two simple approximations—either that the transport cross section of water has a 1/υ behavior, or that the transport cross section is determined from the Radkowsky prescription.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

DOE OSTI2017

Marine Corps Base Camp Lejeune Geospatial Marsh Model: Elevation Maps

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of encroachment pressures in surrounding areas, impairments due to other anthropogenic disturbances, and changes in climate and sea level. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and also optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the second 5 years of the program (DCERP2) are presented in the DCERP2 Final Report.There were four overarching objectives of DCERP2. The first objective was to understand the effects of climate change impacts, including warming temperatures, variability in the hydrological cycle, storm events, and sea level rise on the coastal ecosystems at MCBCL from observations and measurements made over the 10-year program. The second objective was to understand the carbon cycle of the coastal and terrestrial ecosystems at MCBCL through a highly integrated sampling program. The third objective was to develop models, tools, and indicators to evaluate current and projected future ecosystem state changes and translate scientific findings into actionable information for installation managers. The last objective was to recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains PNGs of elevation maps (w/ scale bars) for Freeman, Trapps, and French marsh modeling domains in the target years 2013 (initial), 2035, 2065, & 2100 after modeling marsh under the five sea level rise (SLR) scenarios provided by the base.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2010

Marine Corps Base Camp Lejeune Greenness Gain from 1984 to 1998

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of development pressures in surrounding areas, impairments due to other anthropogenic disturbances, and increasing requirements for compliance with environmental regulations. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and to optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a minimum 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the first six years of the program (DCERP1) are presented here.The overarching objectives of DCERP are to: (1) understand the effects of military training activities, infrastructure development, and other anthropogenic stressors, as well as natural disturbances, on the coastal ecosystems at MCBCL and other coastal military installations; (2) develop models, tools, and indicators to evaluate ecosystem health; and (3) recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains Gain of Green Vegetation data between 1984 and 1998 from Landsat TM5 images. Results are based on the difference in the Tassle Cap greenness band recorded in 1984 to the greenness recorded in 1998. This Intensity of green gain was then developed into a four category green gain classification using the mean and standard deviation of the green gain difference data set standardized to the 1984-2007 change period: 0. No green gain, green loss, or background 1. No significant change; values > 0 to =< 0.02147 (mean) 2. Moderate green gain, thicker veg, etc; values > 0.02147 to =< 0.0525 (mean + SD) 3. Large green gain; values > 0.0525 to max Classes 2 and 3 are retained as significant green gain locations. Polygon patches are labeled with the intensity value in the attribute 'GRIDCODE'. Greenness gain in MCBCL is largely timber harvest areas that have regrown.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2017

Marine Corps Base Camp Lejeune Geospatial Marsh Model: Trap's Bay Output

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of encroachment pressures in surrounding areas, impairments due to other anthropogenic disturbances, and changes in climate and sea level. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and also optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the second 5 years of the program (DCERP2) are presented in the DCERP2 Final Report.There were four overarching objectives of DCERP2. The first objective was to understand the effects of climate change impacts, including warming temperatures, variability in the hydrological cycle, storm events, and sea level rise on the coastal ecosystems at MCBCL from observations and measurements made over the 10-year program. The second objective was to understand the carbon cycle of the coastal and terrestrial ecosystems at MCBCL through a highly integrated sampling program. The third objective was to develop models, tools, and indicators to evaluate current and projected future ecosystem state changes and translate scientific findings into actionable information for installation managers. The last objective was to recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains geospatial data of habitat and elevation as ascii and tabular output (.xlxs) for each site under each SLR scenario for the years 2013 (initial), 2035, 2065, & 2100.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2010

Marine Corps Base Camp Lejeune Daily Sightings Wilson Plover

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2011

Marine Corps Base Camp Lejeune Onslow Bay Seismic Sub-bottom

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of development pressures in surrounding areas, impairments due to other anthropogenic disturbances, and increasing requirements for compliance with environmental regulations. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and to optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a minimum 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the first six years of the program (DCERP1) are presented here.The overarching objectives of DCERP are to: (1) understand the effects of military training activities, infrastructure development, and other coastal military installations; (2) develop models, tools, and indicators to evaluate ecosystem health; and (3) recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package includes seismic sub-bottom profile data are from the shallow (-2 to -11.5m, NAVD88) nearshore region of Onslow Beach. Data were collected in 2007 using a high-resolution Edgetech Chirp 512i (average resolution of 10-20cm). The position of each data point was related to NAD83 using RTK-GPS. Data were collected at a frequency range of 0.5-8 kHz. Heave is apparent in the seismic profiles because no swell filter was applied during acquisition or post-processing. Post-processing was performed using Chesapeake Technology SonarWiz MAP5. Georeferenced .sd files (datums: UTM, Zone 18N, meters & NAVD88, meters) were generated using IVS Fledermaus Pro ver. 7.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2018

Marine Corps Base Camp Lejeune Red Cockaded Woodpecker habitat quality

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of encroachment pressures in surrounding areas, impairments due to other anthropogenic disturbances, and changes in climate and sea level. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and also optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the second 5 years of the program (DCERP2) are presented in the DCERP2 Final Report.There were four overarching objectives of DCERP2. The first objective was to understand the effects of climate change impacts, including warming temperatures, variability in the hydrological cycle, storm events, and sea level rise on the coastal ecosystems at MCBCL from observations and measurements made over the 10-year program. The second objective was to understand the carbon cycle of the coastal and terrestrial ecosystems at MCBCL through a highly integrated sampling program. The third objective was to develop models, tools, and indicators to evaluate current and projected future ecosystem state changes and translate scientific findings into actionable information for installation managers. The last objective was to recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains ranking of red cockaded woodpecker habitat quality by monitoring site. Years scored: 2009, 2010, 2011.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2017

Marine Corps Base Camp Lejeune Geospatial Marsh Model: French Output

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of encroachment pressures in surrounding areas, impairments due to other anthropogenic disturbances, and changes in climate and sea level. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and also optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the second 5 years of the program (DCERP2) are presented in the DCERP2 Final Report.There were four overarching objectives of DCERP2. The first objective was to understand the effects of climate change impacts, including warming temperatures, variability in the hydrological cycle, storm events, and sea level rise on the coastal ecosystems at MCBCL from observations and measurements made over the 10-year program. The second objective was to understand the carbon cycle of the coastal and terrestrial ecosystems at MCBCL through a highly integrated sampling program. The third objective was to develop models, tools, and indicators to evaluate current and projected future ecosystem state changes and translate scientific findings into actionable information for installation managers. The last objective was to recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains geospatial data of habitat and elevation as ascii and tabular output (.xlxs) for each site under each SLR scenario for the years 2013 (initial), 2035, 2065, & 2100.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2018

Marine Corps Base Camp Lejeune Red Cockaded Woodpecker Model

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of encroachment pressures in surrounding areas, impairments due to other anthropogenic disturbances, and changes in climate and sea level. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and also optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the second 5 years of the program (DCERP2) are presented in the DCERP2 Final Report.There were four overarching objectives of DCERP2. The first objective was to understand the effects of climate change impacts, including warming temperatures, variability in the hydrological cycle, storm events, and sea level rise on the coastal ecosystems at MCBCL from observations and measurements made over the 10-year program. The second objective was to understand the carbon cycle of the coastal and terrestrial ecosystems at MCBCL through a highly integrated sampling program. The third objective was to develop models, tools, and indicators to evaluate current and projected future ecosystem state changes and translate scientific findings into actionable information for installation managers. The last objective was to recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains the files needed to run MCBCL simulations using the state-and-transition simulation model (ST-Sim) software to simulate longleaf pine landscape.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2010

Marine Corps Base Camp Lejeune Shorebird Productivity Log 2008-2009

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2010

Marine Corps Base Camp Lejeune Greenness Loss from 1984 to 2009

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of development pressures in surrounding areas, impairments due to other anthropogenic disturbances, and increasing requirements for compliance with environmental regulations. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and to optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a minimum 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the first six years of the program (DCERP1) are presented here.The overarching objectives of DCERP are to: (1) understand the effects of military training activities, infrastructure development, and other coastal military installations; (2) develop models, tools, and indicators to evaluate ecosystem health; and (3) recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains loss of Green Vegetation data between 1984 and 2009 from Landsat TM5 images. Results are based on the difference in the Tassle Cap greenness band recorded in 1984 to the greenness recorded in 2009. This Intensity of green loss was then developed into a five category green loss classification using the mean and standard deviation of the green loss difference data set, standardized to the 1984-2007 time period: 0. No green loss, green gain, or background 1. No significant change; values > 0 to =< 0.0035 (mean - SD) 2. Light green loss, reduced forest, impacted veg, etc; values > 0.0035 to =< 0.04556 (mean) 3. Moderate green loss; values > 0.04556 to =< 0.0876 (mean + SD) 4. Heavy green loss; values > 0.0876 to max. Classes 2,3, and 4 are retained as significant green loss locations. Polygon patches are labeled with the intensity value in the attribute 'GRIDCODE'. Greenness loss in this area is a good indicator of human impact and development.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2010

Marine Corps Base Camp Lejeune Greenness Gain from 1984 to 2009

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of development pressures in surrounding areas, impairments due to other anthropogenic disturbances, and increasing requirements for compliance with environmental regulations. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and to optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a minimum 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the first six years of the program (DCERP1) are presented here.The overarching objectives of DCERP are to: (1) understand the effects of military training activities, infrastructure development, and other coastal military installations; (2) develop models, tools, and indicators to evaluate ecosystem health; and (3) recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains Gain of Green Vegetation information between 1984 and 2009 from Landsat TM5 images. Results are based on the difference in the Tassle Cap greenness band recorded in 1984 to the greenness recorded in 2009. This Intensity of green gain was then developed into a four category green gain classification using the mean and standard deviation of the green gain difference data set standardized to the 1984-2007 change period: 0. No green gain, green loss, or background 1. No significant change; values > 0 to =< 0.02147 (mean) 2. Moderate green gain, thicker veg, etc; values > 0.02147 to =< 0.0525 (mean + SD) 3. Large green gain; values > 0.0525 to max Classes 2 and 3 are retained as significant green gain locations. Polygon patches are labeled with the intensity value in the attribute 'GRIDCODE'. Greenness gain in MCBCL is largely timber harvest areas that have regrown.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2010

Marine Corps Base Camp Lejeune Greenness Loss from 1984 to 1990

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of development pressures in surrounding areas, impairments due to other anthropogenic disturbances, and increasing requirements for compliance with environmental regulations. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and to optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a minimum 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the first six years of the program (DCERP1) are presented here.The overarching objectives of DCERP are to: (1) understand the effects of military training activities, infrastructure development, and other coastal military installations; (2) develop models, tools, and indicators to evaluate ecosystem health; and (3) recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package contains Loss of Green Vegetation data between 1984 and 1990 from Landsat TM5 images. Results are based on the difference in the Tassle Cap greenness band recorded in 1984 and the greenness recorded in 1990. This Intensity of green loss was then developed into a five category green loss classification using the mean and standard deviation of the green loss difference data set, standardized to the 1984-2007 time period: 0. No green loss, green gain, or background 1. No significant change; values > 0 to =< 0.0035 (mean - SD) 2. Light green loss, reduced forest, impacted veg, etc; values > 0.0035 to =< 0.04556 (mean) 3. Moderate green loss; values > 0.04556 to =< 0.0876 (mean + SD) 4. Heavy green loss; values > 0.0876 to max. Classes 2, 3, and 4 are retained as significant green loss locations. Polygon patches are labeled with the intensity value in the attribute 'GRIDCODE'. Greenness loss in this area is a good indicator of human impact and development.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2010

Marine Corps Base Camp Lejeune Greenness Loss from 1984 to 2005

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of development pressures in surrounding areas, impairments due to other anthropogenic disturbances, and increasing requirements for compliance with environmental regulations. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and to optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a minimum 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the first six years of the program (DCERP1) are presented here.The overarching objectives of DCERP are to: (1) understand the effects of military training activities, infrastructure development, and other coastal military installations; (2) develop models, tools, and indicators to evaluate ecosystem health; and (3) recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.This data package measures Loss of Green Vegetation between 1984 and 2005 from Landsat TM5 images. Results are based on the difference in the Tassle Cap greenness band recorded in 1984 and the greenness recorded in 2005. This Intensity of green loss was then developed into a five category green loss classification using the mean and standard deviation of the green loss difference data set, standardized to the 1984-2007 time period: 0. No green loss, green gain, or background 1. No significant change; values > 0 to =< 0.0035 (mean - SD) 2. Light green loss, reduced forest, impacted veg, etc; values > 0.0035 to =< 0.04556 (mean) 3. Moderate green loss; values > 0.04556 to =< 0.0876 (mean + SD) 4. Heavy green loss; values > 0.0876 to max. Classes 2, 3, and 4 are retained as significant green loss locations. Polygon patches are labeled with the intensity value in the attribute 'GRIDCODE'. Greenness loss in this area is a good indicator of human impact and development.

54 ENVIRONMENTAL SCIENCES↗