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At least 847 records · Page 47

Antiproliferative effect of elevated glucose in human microvascular endothelial cells

Diabetic microangiopathy has been implicated as a fundamental feature of the pathological complications of diabetes including retinopathy, neuropathy, and diabetic foot ulceration. However, previous studies devoted to examining the deleterious effects of elevated glucose on the endothelium have been performed largely in primary cultured cells of macrovessel origin. Difficulty in the harvesting and maintenance of microvascular endothelial cells in culture have hindered the study of this relevant population. Therefore, the objective of this study was to characterize the effect of elevated glucose on the proliferation and involved signaling pathways of an immortalized human dermal microvascular endothelial cell line (HMEC-1) that possess similar characteristics to their in vivo counterparts. Human dermal microvascular endothelial cells (HMEC-1) were grown in the presence of normal (5 mM) or high D-glucose (20 mM) for 14 days. The proliferative response of HMEC-1 was compared under these conditions as well as the cAMP and PKC pathways by in vitro assays. Elevated glucose significantly inhibited (P < 0.05) HMEC-1 proliferation after 7, 10, and 14 days. This effect was not mimicked by 20 mM mannitol. The antiproliferative effect was more pronounced with longer exposure (1-14 days) to elevated glucose and was irreversible 4 days after a 10-day exposure. The antiproliferative effect was partially reversed in the presence of a PKA inhibitor, Rp-cAMP (10-50 microM), and/or a PKC inhibitor, Calphostin C (10 nM). HMEC-1 exposed to elevated glucose (20 mM) for 14 days caused an increase in cyclic AMP accumulation, PKA, and PKC activity but was not associated with the activation of downstream events such as CRE and AP-1 binding activity. These data support the hypothesis that HMEC-1 is a suitable model to study the deleterious effects of elevated glucose on microvascular endothelial cells. Continued studies with HMEC-1 may prove advantageous in delineation of the molecular pathophysiology associated with diabetic microangiopathy.

NASA Discipline Cell Biology↗

Feasibility Activities Completed for the Direct Data Distribution (D(sup )3) Experiment

The Direct Data Distribution (D(sup 3)) project being designed at the NASA Glenn Research Center at Lewis Field will demonstrate a high-performance communications system that transmits information at up to 1.2 gigabits per second (Gbps) from an advanced technology payload carried by the space shuttles in low Earth orbit to small (0.9-m) autonomously tracking terminals on the Earth. The flight communications package features a solid-state, phased-array antenna operating in the commercial K-band frequency that electronically steers two independently controlled downlink beams toward low-cost tracking ground terminals. The array enables agile, vibration-free beam steering at reduced size and weight with increased reliability over traditional mechanically steered reflectors. The flight experiment will also demonstrate efficient digital modulation technology that allows transmission of substantially increased amounts of latency-tolerant data (up to 72 Gb of data per minute of contact time) with very high quality (10(exp -11) bit error rate). D(sup 3) enables transmission from low-Earth-orbit science spacecraft, the shuttles, or the International Space Station directly to NASA field centers and principle investigator sites, or directly into the commercial terrestrial telecommunications network for remote distribution and archive. The ground terminal features a cryocooled receiver for ultralow noise and a reduced antenna aperture as well as open-loop tracking for unattended operations. The D(sup 3) technology validation and service demonstration will help to facilitate NASA's transition from using Government-owned communications assets to using commercially provided services.

Wald, Lawrence W.↗

Relocation of Advanced Water Vapor Radiometer 1 to Deep Space Station 55

In June of 2004, the Advanced Water Vapor Radiometer (AWVR) unit no. 1 was relocated to the Deep Space Station (DSS) 55 site in Madrid, Spain, from DSS 25 in Goldstone, California. This article summarizes the relocation activity and the subsequent operation and data acquisition. This activity also relocated the associated Microwave Temperature Profiler (MTP) and Surface Meteorology (SurfMET) package that collectively comprise the Cassini Media Calibration System (MCS).

Oswald, J.↗

The Development of New Solar Indices for use in Thermospheric Density Modeling

New solar indices have been developed to improve thermospheric density modeling for research and operational purposes. Out of 11 new and 4 legacy indices and proxies, we have selected three (F10.7, S10.7, and M10.7) for use in the new JB2006 empirical thermospheric density model. In this work, we report on the development of these solar irradiance indices. The rationale for their use, their definitions, and their characteristics, including the ISO 21348 spectral category and sub-category, wavelength range, solar source temperature region, solar source feature, altitude region of terrestrial atmosphere absorption at unit optical depth, and terrestrial atmosphere thermal processes in the region of maximum energy absorption, are described. We also summarize for each solar index, the facility and instrument(s) used to observe the solar emission, the time frame over which the data exist, the measurement cadence, the data latency, and the research as well as operational availability. The new solar indices are provided in forecast (http://SpaceWx.com) as well as real-time and historical (http://sol.spacenvironment.net/~jb2006/) time frames. We describe the forecast methodology, compare results with actual data for active and quiet solar conditions, and compare improvements in F10.7 forecasting with legacy High Accuracy Satellite Drag Model (HASDM) and NOAA SEC forecasts.

Tobiska, W. Kent↗

Rapid, Repeat-sample Monitoring of Crustal Deformations and Environmental Phenomena with the Uninhabited Aerial Vehicle Synthetic Aperture Radar

The Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) is a precision repeat-pass Interferometric Synthetic Aperture Radar (InSAR) mission being developed by the Jet Propulsion Laboratory and the Dryden Flight Research Center in support of NASA s Science Mission Directorate. UAVSAR's unique ability to fly a repeatable flight path, along with an electronically steerable array, allows interferometric data to be obtained with accuracies measured in millimeters. Deploying the radar on an airborne platform will also allow for radar images to be collected and compared with images from the same area taken hours or even years later - providing for long-term trending and near real-time notification of changes and deformations. UAVSAR s data processing algorithms will provide for near-real time data reduction providing disaster planning and response teams with highly accurate data to aid in the prediction of, and response to, natural phenomena. UAVSAR data can be applied to increasing our understanding of the processes behind solid earth, cryosphere, carbon cycle and other areas of interest in earth science. Technologies developed for UAVSAR may also be applicable to a future earth-orbiting InSAR mission and possibly for missions to the Moon or Mars. The UAVSAR is expected to fly on a Gulfstream III aircraft this winter, followed by a flight test program lasting until the second half of 2007. Following radar calibration and data reduction activities, the platform will be ready for science users in the summer of 2008.

Smith, Robert C.↗

Cascade Distillation Subsystem Development: Progress Toward a Distillation Comparison Test

Recovery of potable water from wastewater is essential for the success of long-duration manned missions to the Moon and Mars. Honeywell International and a team from NASA Johnson Space Center (JSC) are developing a wastewater processing subsystem that is based on centrifugal vacuum distillation. The wastewater processor, referred to as the Cascade Distillation Subsystem (CDS), utilizes an innovative and efficient multistage thermodynamic process to produce purified water. The rotary centrifugal design of the system also provides gas/liquid phase separation and liquid transport under microgravity conditions. A five-stage subsystem unit has been designed, built, delivered and integrated into the NASA JSC Advanced Water Recovery Systems Development Facility for performance testing. A major test objective of the project is to demonstrate the advancement of the CDS technology from the breadboard level to a subsystem level unit. An initial round of CDS performance testing was completed in fiscal year (FY) 2008. Based on FY08 testing, the system is now in development to support an Exploration Life Support (ELS) Project distillation comparison test expected to begin in early 2009. As part of the project objectives planned for FY09, the system will be reconfigured to support the ELS comparison test. The CDS will then be challenged with a series of human-gene-rated waste streams representative of those anticipated for a lunar outpost. This paper provides a description of the CDS technology, a status of the current project activities, and data on the system s performance to date.

Callahan, M. R.↗

Sensitivity of Short-Term Weather Forecasts to Assimilated AIRS Data: Implications for NPOESS Applications

The Atmospheric Infrared Sounder (AIRS) is acting as a heritage and risk reduction instrument for the Cross-track lnfrared Sounder (CrIS) to be flown aboard the NPP and NPOESS satellites. The hyperspectral nature of AIRS and CrIS provides high-quality soundings that, along with their asynoptic observation time over North America, make them attractive sources to fill the spatial and temporal data voids in upper air temperature and moisture measurements for use in data assimilation and numerical weather prediction. Observations from AlRS can be assimilated either as direct radiances or retrieved thermodynamic profiles, and the Short-Term Prediction Research and Transition (SPORT) Center at NASA's Marshall Space Flight Center has used both data types to improve short-term (0-48h), regional forecasts. The purpose of this paper is to share SPORT'S experiences using AlRS radiances and retrieved profiles in regional data assimilation activities by showing that proper handling of issues-including cloud contamination and land emissivity characterization-are necessary to produce optimal analyses and forecasts.

Zavodsky, Bradley↗

802.16e System Profile for NASA Extra-Vehicular Activities

This report identifies an 802.16e system profile that is applicable to a lunar surface wireless network, and specifically for meeting extra-vehicular activity (EVA) data flow requirements. EVA suit communication needs are addressed. Design-driving operational scenarios are considered. These scenarios are then used to identify a configuration of the 802.16e system (system profile) that meets EVA requirements, but also aim to make the radio realizable within EVA constraints. Limitations of this system configuration are highlighted. An overview and development status is presented by Toyon Research Corporation concerning the development of an 802.16e compatible modem under NASA s Small Business Innovative Research (SBIR) Program. This modem is based on the recommended system profile developed as part of this report. Last, a path forward is outlined that presents an evolvable solution for the EVA radio system and lunar surface radio networks. This solution is based on a custom link layer, and 802.16e compliant physical layer compliant to the identified system profile, and a later progression to a fully interoperable 802.16e system.

Foore, Lawrence R.↗

Wireless Avionics Packet to Support Fault Tolerance for Flight Applications

In this protocol and packet format, data traffic is monitored by all network interfaces to determine the health of transmitter and subsystems. When failures are detected, the network inter face applies its recover y policies to provide continued service despite the presence of faults. The protocol, packet format, and inter face are independent of the data link technology used. The current demonstration system supports both commercial off-the-shelf wireless connections and wired Ethernet connections. Other technologies such as 1553 or serial data links can be used for the network backbone. The Wireless Avionics packet is divided into three parts: a header, a data payload, and a checksum. The header has the following components: magic number, version, quality of service, time to live, sending transceiver, function code, payload length, source Application Data Interface (ADI) address, destination ADI address, sending node address, target node address, and a sequence number. The magic number is used to identify WAV packets, and allows the packet format to be updated in the future. The quality of service field allows routing decisions to be made based on this value and can be used to route critical management data over a dedicated channel. The time to live value is used to discard misrouted packets while the source transceiver is updated at each hop. This information is used to monitor the health of each transceiver in the network. To identify the packet type, the function code is used. Besides having a regular data packet, the system supports diagnostic packets for fault detection and isolation. The payload length specifies the number of data bytes in the payload, and this supports variable-length packets in the network. The source ADI is the address of the originating interface. This can be used by the destination application to identify the originating source of the packet where the address consists of a subnet, subsystem class within the subnet, a subsystem unit, and the local ADI number. The destination ADI is used to route the packet to its ultimate destination. At each hop, the sending interface uses the destination address to determine the next node for the data. The sending node is the node address of the interface that is broadcasting the packet. This field is used to determine the health of the subsystem that is sending the packet. In the case of a packet that traverses several intermediate nodes, it may be the node address of the intermediate node. The target node is the node address of the next hop for the packet. It may be an intermediate node, or the final destination for the packet. The sequence number is used to identify duplicate packets. Because each interface has multiple transceivers, the same packet will appear at both receivers. The sequence number allows the interface to correlate the reception and forward a single, unique packet for additional processing. The subnet field allows data traffic to be partitioned into segregated local networks to support large networks while keeping each subnet at a manageable size. This also keeps the routing table small enough so routing can be done by a simple table lookup in an FPGA device. The subsystem class identifies members of a set of redundant subsystems, and, in a hot standby configuration, all members of the subsystem class will receive the data packets. Only the active subsystem will generate data traffic. Specific units in a class of redundant units can be identified and, if the hot standby configuration is not used, packets will be directed to a specific subsystem unit.

Block, Gary L.↗

Visual Data Comm: A Tool for Visualizing Data Communication in the Multi Sector Planner Study

Data comm is a new technology proposed in future air transport system as a potential tool to provide comprehensive data connectivity. It is a key enabler to manage 4D trajectory digitally, potentially resulting in improved flight times and increased throughput. Future concepts with data comm integration have been tested in a number of human-in-the-loop studies but analyzing the results has proven to be particularly challenging because future traffic environment in which data comm is fully enabled has assumed high traffic density, resulting in data set with large amount of information. This paper describes the motivation, design, current and potential future application of Visual Data Comm (VDC), a tool for visualizing data developed in Java using Processing library which is a tool package designed for interactive visualization programming. This paper includes an example of an application of VDC on data pertaining to the most recent Multi Sector Planner study, conducted at NASA s Airspace Operations Laboratory in 2009, in which VDC was used to visualize and interpret data comm activities

Lee, Hwasoo Eric↗

NASA Tech Briefs, November 2004

Topics include: Multifunction Imaging and Spectroscopic Instrument; Position-Finding Instrument Built Around a Magnetometer; Improved Measurement of Dispersion in an Optical Fiber; Probe for Sampling of Interstitial Fluid From Bone; Neuropsychological Testing of Astronauts; Method of Calibration for a Large Cathetometer System; Four-Channel PC/104 MIL-STD-1553 Circuit Board; Improved Method of Locating Defects in Wiring Insulation; Strobe Traffic Lights Warn of Approaching Emergency Vehicles; Improved Timing Scheme for Spaceborne Precipitation Radar; Concept for Multiple-Access Free-Space Laser Communications; Variable Shadow Screens for Imaging Optical Devices; Verifying Diagnostic Software; Initial Processing of Infrared Spectral Data; Activity-Centric Approach to Distributed Programming; Controlling Distributed Planning; New Material for Surface-Enhanced Raman Spectroscopy; Treated Carbon Nanofibers for Storing Energy in Aqueous KOH; Advanced Infant Car Seat Would Increase Highway Safety; Development of Biomorphic Flyers; Second-Generation Six-Limbed Experimental Robot; Miniature Linear Actuator for Small Spacecraft; Process for Making Single-Domain Magnetite Crystals; A New Process for Fabricating Random Silicon Nanotips; Resin-Transfer-Molding of a Tool Face; Improved Phase-Mask Fabrication of Fiber Bragg Gratings; Tool for Insertion of a Fiber-Optic Terminus in a Connector; Nanofluidic Size-Exclusion Chromatograph; Lightweight, Low-CTE Tubes Made From Biaxially Oriented LCPs; Using Redundancy To Reduce Errors in Magnetometer Readings; Compact Instrument for Measuring Profile of a Light Beam; Multilayer Dielectric Transmissive Optical Phase Modulator; Second-Generation Multi-Angle Imaging Spectroradiometer; Real-Time Adaptive Color Segmentation by Neural Networks; Research and Development in Optical Communications; Tests of Multibeam Scintillation Mitigation on Laser Uplinks; and Spaceborne Infrared Atmospheric Sounder.

Source record↗

SpaceCube Mini

This version of the SpaceCube will be a full-fledged, onboard space processing system capable of 2500+ MIPS, and featuring a number of plug-andplay gigabit and standard interfaces, all in a condensed 3x3x3 form factor [less than 10 watts and less than 3 lb (approximately equal to 1.4 kg)]. The main processing engine is the Xilinx SIRF radiation- hardened-by-design Virtex-5 FX-130T field-programmable gate array (FPGA). Even as the SpaceCube 2.0 version (currently under test) is being targeted as the platform of choice for a number of the upcoming Earth Science Decadal Survey missions, GSFC has been contacted by customers who wish to see a system that incorporates key features of the version 2.0 architecture in an even smaller form factor. In order to fulfill that need, the SpaceCube Mini is being designed, and will be a very compact and low-power system. A similar flight system with this combination of small size, low power, low cost, adaptability, and extremely high processing power does not otherwise exist, and the SpaceCube Mini will be of tremendous benefit to GSFC and its partners. The SpaceCube Mini will utilize space-grade components. The primary processing engine of the Mini is the Xilinx Virtex-5 SIRF FX-130T radiation-hardened-by-design FPGA for critical flight applications in high-radiation environments. The Mini can also be equipped with a commercial Xilinx Virtex-5 FPGA with integrated PowerPCs for a low-cost, high-power computing platform for use in the relatively radiation- benign LEOs (low-Earth orbits). In either case, this version of the Space-Cube will weigh less than 3 pounds (.1.4 kg), conform to the CubeSat form-factor (10x10x10 cm), and will be low power (less than 10 watts for typical applications). The SpaceCube Mini will have a radiation-hardened Aeroflex FPGA for configuring and scrubbing the Xilinx FPGA by utilizing the onboard FLASH memory to store the configuration files. The FLASH memory will also be used for storing algorithm and application code for the PowerPCs and the Xilinx FPGA. In addition, it will feature highspeed DDR SDRAM (double data rate synchronous dynamic random-access memory) to store the instructions and data of active applications. This version will also feature SATA-II and Gigabit Ethernet interfaces. Furthermore, there will also be general-purpose, multi-gigabit interfaces. In addition, the system will have dozens of transceivers that can support LVDS (low-voltage differential signaling), RS-422, or SpaceWire. The SpaceCube Mini includes an I/O card that can be customized to meet the needs of each mission. This version of the SpaceCube will be designed so that multiple Minis can be networked together using SpaceWire, Ethernet, or even a custom protocol. Scalability can be provided by networking multiple SpaceCube Minis together. Rigid-Flex technology is being targeted for the construction of the SpaceCube Mini, which will make the extremely compact and low-weight design feasible. The SpaceCube Mini is designed to fit in the compact CubeSat form factor, thus allowing deployment in a new class of missions that the previous SpaceCube versions were not suited for. At the time of this reporting, engineering units should be available in the summer 2012.

Michael Lin↗

Matera CGS VLBI Analysis Center

This paper reports the VLBI data analysis activities at the Space Geodesy Center (CGS), Matera, from January 2012 through December 2012, and the contributions that the CGS intends to provide for the future as an IVS Analysis Center.

Lanotte, Roberto↗

Sea Surface Salinity and Wind Retrieval Algorithm Using Combined Passive-Active L-Band Microwave Data

Aquarius is a combined passive/active L-band microwave instrument developed to map the salinity field at the surface of the ocean from space. The data will support studies of the coupling between ocean circulation, the global water cycle, and climate. The primary science objective of this mission is to monitor the seasonal and interannual variation of the large scale features of the surface salinity field in the open ocean with a spatial resolution of 150 kilometers and a retrieval accuracy of 0.2 practical salinity units globally on a monthly basis. The measurement principle is based on the response of the L-band (1.413 gigahertz) sea surface brightness temperatures (T (sub B)) to sea surface salinity. To achieve the required 0.2 practical salinity units accuracy, the impact of sea surface roughness (e.g. wind-generated ripples and waves) along with several factors on the observed brightness temperature has to be corrected to better than a few tenths of a degree Kelvin. To the end, Aquarius includes a scatterometer to help correct for this surface roughness effect.

ocean salinity↗

The Continuing Exploration of Saturn by Cassini

The Cassini/Huygens mission to explore the Saturn system is a joint international endeavor among NASA, the European Space Agency (ESA), and the Italian Space Agency. The original mission as agreed to and funded by the partner agencies was for four years of active science data collection from in orbit about Saturn, which began on July 1, 2004. When this part of the mission was completed and the spacecraft was still in an excellent state of health with much valuable science still remaining to be accomplished, a mission extension of 27 months was approved. This first extension is now more than half completed and a proposal is being developed for a further extension with one of the primary science goals being the observation and study of seasonal change at Saturn as it moves into summer in its northern hemisphere for the first time since Cassini arrived. This paper describes highlights of the mission to date, summarizes the current spacecraft health and status of its consumables, and gives an overview of what is planned for further exploration of the Saturn system by the Cassini Project.

Mitchell, Robert T.↗